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GFFUTR.pm
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package GFFUTR;
use strict 'vars';
use strict 'refs';
require GFFTranscript;
require GFFExon;
# use overload '==' => \&compare;
# type = [five_prime_utr,three_prime_utr]
sub new {
my ( $exon_name, $start_coord, $end_coord, $type, $parent ) = @_;
my $self = { id => $exon_name,
start => $start_coord,
end => $end_coord,
type => $type,
parent => $parent };
bless $self, GFFUTR;
return $self;
}
sub copy {
my ( $ref, $parent ) = @_;
my $self = {
id => $ref->{id},
start => $ref->{start},
end => $ref->{end},
type => $ref->{type},
parent => $parent
};
bless $self, GFFUTR;
# Copying other atrributes
$self->cpy_attrib($ref);
return $self;
}
sub cpy_attrib{
my $self = shift;
my ( $ref ) = @_;
foreach my $ckey ( keys %{$ref->{attrib}} ){
$self->{attrib}{$ckey} = $ref->{attrib}{$ckey}
}
}
# The concept of UTR in the context of GFF is different
# then the biological concept. The UTR is associated to an exon,
# Example:
# | = coding
# : = UTR
# - = intron
#
# ||||||||||::::----------::::::::::::
# ^^^^ this UTR is considered internal and cannot be removed
sub is_internal {
my $self = shift;
my $transcript = $self->{parent}->get_parent();
return 0 if ( $self->get_start() == $transcript->get_start() || $self->get_end() == $transcript->get_end() );
return 1;
}
# type = [five_prime_utr,three_prime_utr]
sub get_type {
my $self = shift;
return $self->{type};
}
sub get_type_GTF {
my $self = shift;
return '5UTR' if $self->{type} eq 'five_prime_utr';
return '3UTR' if $self->{type} eq 'three_prime_utr';
}
sub get_parent {
my $self = shift;
return $self->{parent};
}
sub set_id {
my $self = shift;
my ($new_id) = @_;
$self->{id} = $new_id;
}
sub get_id {
my $self = shift;
return $self->{id};
}
sub get_start {
my $self = shift;
return $self->{start};
}
sub get_end {
my $self = shift;
return $self->{end};
}
sub get_length {
my $self = shift;
return $self->{end} - $self->{start} + 1;
}
sub set_start {
my $self = shift;
my ( $coord_start ) = @_;
return $self->{start} = $coord_start;
}
sub set_end {
my $self = shift;
my ( $coord_end ) = @_;
return $self->{end} = $coord_end;
}
sub set_parent_exon{
my $self = shift;
my ( $ref_exon ) = @_;
$self->{exon} = $ref_exon;
}
sub get_parent_exon{
my $self = shift;
return $self->{exon};
}
sub toGFF {
my $self = shift;
my ($chrom, $parent, $strand ) = @_;
my $str = $chrom . "\t.\t" . $self->{type} . "\t" .
$self->{start} . "\t" .
$self->{end} . "\t.\t" .
$strand . "\t.\t" .
"ID=" . $self->{id} . ";" .
"Parent=$parent;\n";
return $str;
}
sub toGTF {
my $self = shift;
#print STDERR "UTR\n";
#getc();
my ($chrom, $parent, $strand ) = @_;
my $str = $chrom . "\t.\t" . $self->get_type_GTF() . "\t" .
$self->{start} . "\t" .
$self->{end} . "\t.\t" .
$strand . "\t.\n";
return $str;
}
sub set_attribute {
my $self = shift;
my ( $key, $value ) = @_;
return $self->{attrib}{$key} = $value;
}
sub get_attribute {
my $self = shift;
my ($key) = @_;
return $self->{attrib}{$key};
}
return 1;