From af7d5b77020e46ce63b4efd8872da9c2f9a07aa4 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Thu, 10 Mar 2022 12:15:14 -0500 Subject: [PATCH 001/220] add filename to filter for computed files --- api/data_refinery_api/views/computed_file.py | 1 + 1 file changed, 1 insertion(+) diff --git a/api/data_refinery_api/views/computed_file.py b/api/data_refinery_api/views/computed_file.py index 6e0c2c4d8..0af9fa926 100644 --- a/api/data_refinery_api/views/computed_file.py +++ b/api/data_refinery_api/views/computed_file.py @@ -139,6 +139,7 @@ class ComputedFileListView(generics.ListAPIView): "created_at", "last_modified", "result__id", + "filename", ) ordering_fields = ( "id", From 57071f9a584187b7b3a3bed4626939c5031032d2 Mon Sep 17 00:00:00 2001 From: Deepa Prasad Date: Mon, 11 Apr 2022 13:38:13 -0400 Subject: [PATCH 002/220] changed donate link --- .github/FUNDING.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/FUNDING.yml b/.github/FUNDING.yml index 5194db9a2..66f9b7754 100644 --- a/.github/FUNDING.yml +++ b/.github/FUNDING.yml @@ -9,4 +9,4 @@ community_bridge: # Replace with a single Community Bridge project-name e.g., cl liberapay: # Replace with a single Liberapay username issuehunt: # Replace with a single IssueHunt username otechie: # Replace with a single Otechie username -custom: https://www.ccdatalab.org/donate +custom: https://www.ccdatalab.org/donate-link From f777db3f61f723ab9b46dd1ff5102cc93964a223 Mon Sep 17 00:00:00 2001 From: ark Date: Thu, 5 May 2022 12:03:20 -0700 Subject: [PATCH 003/220] Get rid of wildcard imports. --- .../import_external_sample_attributes.py | 2 +- .../import_external_sample_keywords.py | 6 ++++-- .../management/commands/clear_database.py | 21 ++++++++++++++++++- .../commands/correct_affy_samples.py | 2 +- 4 files changed, 26 insertions(+), 5 deletions(-) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_attributes.py b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_attributes.py index c22a3f354..def44fbdd 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_attributes.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_attributes.py @@ -23,7 +23,7 @@ import botocore from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models import * +from data_refinery_common.models import Contribution, OntologyTerm, Sample, SampleAttribute from data_refinery_common.utils import parse_s3_url logger = get_and_configure_logger(__name__) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py index 434b12ceb..c3b84375a 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py @@ -25,7 +25,7 @@ import botocore from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models import * +from data_refinery_common.models import Contribution, OntologyTerm, Sample, SampleKeyword from data_refinery_common.utils import parse_s3_url logger = get_and_configure_logger(__name__) @@ -56,7 +56,9 @@ def import_keywords(keywords: Dict, source: Contribution): for keyword in keywords: SampleKeyword.objects.get_or_create( - sample=sample, source=source, name=OntologyTerm.get_or_create_from_api(keyword), + sample=sample, + source=source, + name=OntologyTerm.get_or_create_from_api(keyword), ) for experiment in dirty_experiments: diff --git a/foreman/data_refinery_foreman/surveyor/management/commands/clear_database.py b/foreman/data_refinery_foreman/surveyor/management/commands/clear_database.py index 81ea94c7e..a1518c6ae 100644 --- a/foreman/data_refinery_foreman/surveyor/management/commands/clear_database.py +++ b/foreman/data_refinery_foreman/surveyor/management/commands/clear_database.py @@ -4,7 +4,26 @@ from django.core.management.base import BaseCommand -from data_refinery_common.models import * +from data_refinery_common.models import ( + ComputationalResult, + ComputationalResultAnnotation, + ComputedFile, + DownloaderJob, + DownloaderJobOriginalFileAssociation, + Experiment, + ExperimentAnnotation, + ExperimentSampleAssociation, + Organism, + OrganismIndex, + OriginalFile, + ProcessorJob, + ProcessorJobOriginalFileAssociation, + Sample, + SampleAnnotation, + SampleResultAssociation, + SurveyJob, + SurveyJobKeyValue, +) class Command(BaseCommand): diff --git a/workers/data_refinery_workers/processors/management/commands/correct_affy_samples.py b/workers/data_refinery_workers/processors/management/commands/correct_affy_samples.py index 8f5567b66..7de449438 100644 --- a/workers/data_refinery_workers/processors/management/commands/correct_affy_samples.py +++ b/workers/data_refinery_workers/processors/management/commands/correct_affy_samples.py @@ -22,7 +22,7 @@ from rpy2.rinterface_lib.embedded import RRuntimeError from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models import * +from data_refinery_common.models import Sample from data_refinery_common.utils import get_env_variable, get_readable_affymetrix_names logger = get_and_configure_logger(__name__) From 6b606f2013982e78cee31a1bec8f2d6672cbf629 Mon Sep 17 00:00:00 2001 From: ark Date: Thu, 5 May 2022 20:30:59 -0700 Subject: [PATCH 004/220] Fix test_common job. --- workers/install_affy_only.R | 1 + 1 file changed, 1 insertion(+) diff --git a/workers/install_affy_only.R b/workers/install_affy_only.R index 354e0e592..b488a0287 100644 --- a/workers/install_affy_only.R +++ b/workers/install_affy_only.R @@ -8,6 +8,7 @@ options(Ncpus=parallel::detectCores()) devtools::install_version('dplyr', version='1.0.0') devtools::install_version('tidyr', version='1.1.0') devtools::install_version('ff', version='2.2-14') +devtools::install_version('locfit', version='1.5-9.4') # Helper function that installs a list of packages using the input URLs install_with_url <- function(urls) { From 980a8316b3b8dfa1e0f98d937e8e99822ebd4292 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 24 Jun 2022 10:06:50 -0700 Subject: [PATCH 005/220] Add psycopg2 package dependencies. --- common/dockerfiles/Dockerfile.migrations | 5 +++++ foreman/dockerfiles/Dockerfile.foreman | 2 ++ 2 files changed, 7 insertions(+) diff --git a/common/dockerfiles/Dockerfile.migrations b/common/dockerfiles/Dockerfile.migrations index 7c042f255..8688f12f1 100644 --- a/common/dockerfiles/Dockerfile.migrations +++ b/common/dockerfiles/Dockerfile.migrations @@ -3,6 +3,11 @@ FROM python:3.8.5-slim-buster RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user +RUN apt-get update -qq && \ + apt-get install -y \ + gcc \ + libpq-dev + # Needed for migration 0006 COPY config/ config diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 8c09c6888..929ef2476 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -8,6 +8,8 @@ RUN apt-get -y install apt-fast RUN apt-fast update -qq && \ apt-fast install -y \ + gcc \ + libpq-dev \ python3 \ python3-pip From 0836c327d843c3aca5d980b9bc60a93b02f988e0 Mon Sep 17 00:00:00 2001 From: "dependabot[bot]" <49699333+dependabot[bot]@users.noreply.github.com> Date: Thu, 11 Aug 2022 14:51:52 +0000 Subject: [PATCH 006/220] Bump django from 3.2.7 to 3.2.15 in /api Bumps [django](https://github.com/django/django) from 3.2.7 to 3.2.15. - [Release notes](https://github.com/django/django/releases) - [Commits](https://github.com/django/django/compare/3.2.7...3.2.15) --- updated-dependencies: - dependency-name: django dependency-type: direct:production ... Signed-off-by: dependabot[bot] --- api/requirements.txt | 182 ++++++++++++++++++++++++++++++++----------- 1 file changed, 137 insertions(+), 45 deletions(-) diff --git a/api/requirements.txt b/api/requirements.txt index 2bbe81c1e..d3adfa3fa 100644 --- a/api/requirements.txt +++ b/api/requirements.txt @@ -4,51 +4,143 @@ # # pip-compile requirements.in # -apscheduler==3.7.0 # via -r requirements.in -asgiref==3.3.4 # via django -boto3==1.17.93 # via -r requirements.in -botocore==1.20.93 # via boto3, s3transfer -certifi==2021.5.30 # via requests -chardet==4.0.0 # via requests -coreapi==2.3.3 # via -r requirements.in, drf-yasg -coreschema==0.0.4 # via coreapi, drf-yasg -coverage==5.5 # via -r requirements.in -django-computedfields==0.1.5 # via -r requirements.in -django-cors-headers==3.7.0 # via -r requirements.in -django-elasticsearch-dsl-drf==0.22.1 # via -r requirements.in -django-elasticsearch-dsl==6.5.0 # via -r requirements.in, django-elasticsearch-dsl-drf -django-filter==2.4.0 # via -r requirements.in -django-hstore==1.4.2 # via djangorestframework-hstore -django-nine==0.2.4 # via django-elasticsearch-dsl-drf -django==3.2.7 # via -r requirements.in, django-cors-headers, django-filter, django-nine, djangorestframework, djangorestframework-hstore, drf-yasg -djangorestframework-hstore==1.3 # via -r requirements.in -djangorestframework==3.12.4 # via -r requirements.in, django-elasticsearch-dsl-drf, djangorestframework-hstore, drf-yasg -drf-yasg==1.20.0 # via -r requirements.in -elasticsearch-dsl==6.4.0 # via -r requirements.in, django-elasticsearch-dsl, django-elasticsearch-dsl-drf -elasticsearch==6.8.2 # via django-elasticsearch-dsl-drf, elasticsearch-dsl -idna==2.10 # via requests -inflection==0.5.1 # via drf-yasg -itypes==1.2.0 # via coreapi -jinja2==3.0.1 # via -r requirements.in, coreschema -jmespath==0.10.0 # via boto3, botocore -markupsafe==2.0.1 # via -r requirements.in, jinja2 -packaging==20.9 # via drf-yasg -psycopg2-binary==2.8.6 # via -r requirements.in -psycopg2==2.8.6 # via djangorestframework-hstore -pyparsing==2.4.7 # via packaging -python-dateutil==2.8.1 # via botocore, elasticsearch-dsl -pytz==2021.1 # via apscheduler, django, tzlocal -pyyaml==5.4.1 # via -r requirements.in -requests==2.25.1 # via -r requirements.in, coreapi -ruamel.yaml.clib==0.2.2 # via ruamel.yaml -ruamel.yaml==0.17.9 # via drf-yasg -s3transfer==0.4.2 # via boto3 -six==1.16.0 # via -r requirements.in, apscheduler, django-elasticsearch-dsl, django-elasticsearch-dsl-drf, elasticsearch-dsl, python-dateutil -sqlparse==0.4.2 # via -r requirements.in, django -typing-extensions==3.10.0.0 # via asgiref -tzlocal==2.1 # via apscheduler -uritemplate==3.0.1 # via coreapi, drf-yasg -urllib3==1.26.5 # via -r requirements.in, botocore, elasticsearch, requests +apscheduler==3.7.0 + # via -r requirements.in +asgiref==3.3.4 + # via django +boto3==1.17.93 + # via -r requirements.in +botocore==1.20.93 + # via + # boto3 + # s3transfer +certifi==2021.5.30 + # via requests +chardet==4.0.0 + # via requests +coreapi==2.3.3 + # via + # -r requirements.in + # drf-yasg +coreschema==0.0.4 + # via + # coreapi + # drf-yasg +coverage==5.5 + # via -r requirements.in +django==3.2.15 + # via + # -r requirements.in + # django-cors-headers + # django-filter + # django-nine + # djangorestframework + # djangorestframework-hstore + # drf-yasg +django-computedfields==0.1.5 + # via -r requirements.in +django-cors-headers==3.7.0 + # via -r requirements.in +django-elasticsearch-dsl==6.5.0 + # via + # -r requirements.in + # django-elasticsearch-dsl-drf +django-elasticsearch-dsl-drf==0.22.1 + # via -r requirements.in +django-filter==2.4.0 + # via -r requirements.in +django-hstore==1.4.2 + # via djangorestframework-hstore +django-nine==0.2.4 + # via django-elasticsearch-dsl-drf +djangorestframework==3.12.4 + # via + # -r requirements.in + # django-elasticsearch-dsl-drf + # djangorestframework-hstore + # drf-yasg +djangorestframework-hstore==1.3 + # via -r requirements.in +drf-yasg==1.20.0 + # via -r requirements.in +elasticsearch==6.8.2 + # via + # django-elasticsearch-dsl-drf + # elasticsearch-dsl +elasticsearch-dsl==6.4.0 + # via + # -r requirements.in + # django-elasticsearch-dsl + # django-elasticsearch-dsl-drf +idna==2.10 + # via requests +inflection==0.5.1 + # via drf-yasg +itypes==1.2.0 + # via coreapi +jinja2==3.0.1 + # via + # -r requirements.in + # coreschema +jmespath==0.10.0 + # via + # boto3 + # botocore +markupsafe==2.0.1 + # via + # -r requirements.in + # jinja2 +packaging==20.9 + # via drf-yasg +psycopg2==2.8.6 + # via djangorestframework-hstore +psycopg2-binary==2.8.6 + # via -r requirements.in +pyparsing==2.4.7 + # via packaging +python-dateutil==2.8.1 + # via + # botocore + # elasticsearch-dsl +pytz==2021.1 + # via + # apscheduler + # django + # tzlocal +pyyaml==5.4.1 + # via -r requirements.in +requests==2.25.1 + # via + # -r requirements.in + # coreapi +ruamel-yaml==0.17.9 + # via drf-yasg +s3transfer==0.4.2 + # via boto3 +six==1.16.0 + # via + # -r requirements.in + # apscheduler + # django-elasticsearch-dsl + # django-elasticsearch-dsl-drf + # elasticsearch-dsl + # python-dateutil +sqlparse==0.4.2 + # via + # -r requirements.in + # django +tzlocal==2.1 + # via apscheduler +uritemplate==3.0.1 + # via + # coreapi + # drf-yasg +urllib3==1.26.5 + # via + # -r requirements.in + # botocore + # elasticsearch + # requests # The following packages are considered to be unsafe in a requirements file: # setuptools From d654b01c98cc4bc0acf3740b41bf965db0b13ea1 Mon Sep 17 00:00:00 2001 From: "dependabot[bot]" <49699333+dependabot[bot]@users.noreply.github.com> Date: Thu, 11 Aug 2022 14:51:54 +0000 Subject: [PATCH 007/220] Bump django in /workers/data_refinery_workers/downloaders Bumps [django](https://github.com/django/django) from 3.2.7 to 3.2.15. - [Release notes](https://github.com/django/django/releases) - [Commits](https://github.com/django/django/compare/3.2.7...3.2.15) --- updated-dependencies: - dependency-name: django dependency-type: direct:production ... Signed-off-by: dependabot[bot] --- .../downloaders/requirements.txt | 79 +++++++++++++------ 1 file changed, 56 insertions(+), 23 deletions(-) diff --git a/workers/data_refinery_workers/downloaders/requirements.txt b/workers/data_refinery_workers/downloaders/requirements.txt index 8a5a95c18..fe064620f 100644 --- a/workers/data_refinery_workers/downloaders/requirements.txt +++ b/workers/data_refinery_workers/downloaders/requirements.txt @@ -4,26 +4,59 @@ # # pip-compile requirements.in # -asgiref==3.3.4 # via django -certifi==2021.5.30 # via requests -chardet==4.0.0 # via requests -coverage==5.5 # via coveralls -coveralls==3.1.0 # via -r requirements.in -django-computedfields==0.1.5 # via -r requirements.in -django==3.2.7 # via -r requirements.in -docopt==0.6.2 # via coveralls -idna==2.10 # via requests, yarl -multidict==5.1.0 # via yarl -psutil==5.8.0 # via -r requirements.in -psycopg2-binary==2.8.6 # via -r requirements.in -pytz==2021.1 # via django -pyyaml==5.4.1 # via -r requirements.in, vcrpy -requests==2.25.1 # via -r requirements.in, coveralls -retrying==1.3.3 # via -r requirements.in -six==1.16.0 # via retrying, vcrpy -sqlparse==0.4.2 # via -r requirements.in, django -typing-extensions==3.10.0.0 # via asgiref, yarl -urllib3==1.26.5 # via -r requirements.in, requests -vcrpy==4.1.1 # via -r requirements.in -wrapt==1.12.1 # via vcrpy -yarl==1.6.3 # via vcrpy +asgiref==3.3.4 + # via django +certifi==2021.5.30 + # via requests +chardet==4.0.0 + # via requests +coverage==5.5 + # via coveralls +coveralls==3.1.0 + # via -r requirements.in +django==3.2.15 + # via -r requirements.in +django-computedfields==0.1.5 + # via -r requirements.in +docopt==0.6.2 + # via coveralls +idna==2.10 + # via + # requests + # yarl +multidict==5.1.0 + # via yarl +psutil==5.8.0 + # via -r requirements.in +psycopg2-binary==2.8.6 + # via -r requirements.in +pytz==2021.1 + # via django +pyyaml==5.4.1 + # via + # -r requirements.in + # vcrpy +requests==2.25.1 + # via + # -r requirements.in + # coveralls +retrying==1.3.3 + # via -r requirements.in +six==1.16.0 + # via + # retrying + # vcrpy +sqlparse==0.4.2 + # via + # -r requirements.in + # django +urllib3==1.26.5 + # via + # -r requirements.in + # requests +vcrpy==4.1.1 + # via -r requirements.in +wrapt==1.12.1 + # via vcrpy +yarl==1.6.3 + # via vcrpy From 3986cdc24685c7b87c24ebefda1c4c045e973474 Mon Sep 17 00:00:00 2001 From: "dependabot[bot]" <49699333+dependabot[bot]@users.noreply.github.com> Date: Thu, 11 Aug 2022 14:53:37 +0000 Subject: [PATCH 008/220] Bump django from 3.2.7 to 3.2.15 in /common Bumps [django](https://github.com/django/django) from 3.2.7 to 3.2.15. - [Release notes](https://github.com/django/django/releases) - [Commits](https://github.com/django/django/compare/3.2.7...3.2.15) --- updated-dependencies: - dependency-name: django dependency-type: direct:production ... Signed-off-by: dependabot[bot] --- common/requirements.txt | 134 ++++++++++++++++++++++++++++++---------- 1 file changed, 100 insertions(+), 34 deletions(-) diff --git a/common/requirements.txt b/common/requirements.txt index 088feeb37..b8efe2dea 100644 --- a/common/requirements.txt +++ b/common/requirements.txt @@ -4,37 +4,103 @@ # # pip-compile requirements.in # -asgiref==3.3.4 # via django -boto3==1.17.93 # via -r requirements.in -botocore==1.20.93 # via boto3, s3transfer -certifi==2021.5.30 # via requests -chardet==4.0.0 # via requests -coverage==5.5 # via -r requirements.in -daiquiri==3.0.0 # via -r requirements.in -django-computedfields==0.1.5 # via -r requirements.in -django-elasticsearch-dsl-drf==0.22.1 # via -r requirements.in -django-elasticsearch-dsl==6.5.0 # via -r requirements.in, django-elasticsearch-dsl-drf -django-nine==0.2.4 # via django-elasticsearch-dsl-drf -django==3.2.7 # via -r requirements.in, django-nine, djangorestframework -djangorestframework==3.12.4 # via -r requirements.in, django-elasticsearch-dsl-drf -elasticsearch-dsl==6.4.0 # via -r requirements.in, django-elasticsearch-dsl, django-elasticsearch-dsl-drf -elasticsearch==6.8.2 # via django-elasticsearch-dsl-drf, elasticsearch-dsl -idna==2.10 # via requests, yarl -jmespath==0.10.0 # via boto3, botocore -multidict==5.1.0 # via yarl -psycopg2-binary==2.8.6 # via -r requirements.in -python-dateutil==2.8.1 # via botocore, elasticsearch-dsl -python-json-logger==2.0.1 # via daiquiri -pytz==2021.1 # via django -pyyaml==5.4.1 # via -r requirements.in, vcrpy -raven==6.10.0 # via -r requirements.in -requests==2.25.1 # via -r requirements.in -retrying==1.3.3 # via -r requirements.in -s3transfer==0.4.2 # via boto3 -six==1.16.0 # via django-elasticsearch-dsl, django-elasticsearch-dsl-drf, elasticsearch-dsl, python-dateutil, retrying, vcrpy -sqlparse==0.4.2 # via -r requirements.in, django -typing-extensions==3.10.0.0 # via asgiref, yarl -urllib3==1.26.5 # via -r requirements.in, botocore, elasticsearch, requests -vcrpy==4.1.1 # via -r requirements.in -wrapt==1.12.1 # via vcrpy -yarl==1.6.3 # via vcrpy +asgiref==3.3.4 + # via django +boto3==1.17.93 + # via -r requirements.in +botocore==1.20.93 + # via + # boto3 + # s3transfer +certifi==2021.5.30 + # via requests +chardet==4.0.0 + # via requests +coverage==5.5 + # via -r requirements.in +daiquiri==3.0.0 + # via -r requirements.in +django==3.2.15 + # via + # -r requirements.in + # django-nine + # djangorestframework +django-computedfields==0.1.5 + # via -r requirements.in +django-elasticsearch-dsl==6.5.0 + # via + # -r requirements.in + # django-elasticsearch-dsl-drf +django-elasticsearch-dsl-drf==0.22.1 + # via -r requirements.in +django-nine==0.2.4 + # via django-elasticsearch-dsl-drf +djangorestframework==3.12.4 + # via + # -r requirements.in + # django-elasticsearch-dsl-drf +elasticsearch==6.8.2 + # via + # django-elasticsearch-dsl-drf + # elasticsearch-dsl +elasticsearch-dsl==6.4.0 + # via + # -r requirements.in + # django-elasticsearch-dsl + # django-elasticsearch-dsl-drf +idna==2.10 + # via + # requests + # yarl +jmespath==0.10.0 + # via + # boto3 + # botocore +multidict==5.1.0 + # via yarl +psycopg2-binary==2.8.6 + # via -r requirements.in +python-dateutil==2.8.1 + # via + # botocore + # elasticsearch-dsl +python-json-logger==2.0.1 + # via daiquiri +pytz==2021.1 + # via django +pyyaml==5.4.1 + # via + # -r requirements.in + # vcrpy +raven==6.10.0 + # via -r requirements.in +requests==2.25.1 + # via -r requirements.in +retrying==1.3.3 + # via -r requirements.in +s3transfer==0.4.2 + # via boto3 +six==1.16.0 + # via + # django-elasticsearch-dsl + # django-elasticsearch-dsl-drf + # elasticsearch-dsl + # python-dateutil + # retrying + # vcrpy +sqlparse==0.4.2 + # via + # -r requirements.in + # django +urllib3==1.26.5 + # via + # -r requirements.in + # botocore + # elasticsearch + # requests +vcrpy==4.1.1 + # via -r requirements.in +wrapt==1.12.1 + # via vcrpy +yarl==1.6.3 + # via vcrpy From c4e19e75bd7fad17c6611cd438cf87e38b48bbe0 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 1 Sep 2022 10:50:59 -0700 Subject: [PATCH 009/220] Update pre-commit config. Bump black version. --- .pre-commit-config.yaml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index d024704da..b651ce24a 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -22,7 +22,7 @@ repos: - id: isort - repo: https://github.com/psf/black - rev: 19.10b0 + rev: 22.3.0 hooks: - id: black args: [--line-length=100] From b87cbd21675c51dbaa8a956a3c87a1444ccade14 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 1 Sep 2022 11:03:18 -0700 Subject: [PATCH 010/220] Add `Accession` model. Update pre-commit config. --- .pre-commit-config.yaml | 2 +- .../migrations/0071_auto_20220901_1653.py | 44 +++++++++++++++++++ .../data_refinery_common/models/__init__.py | 1 + .../data_refinery_common/models/accession.py | 22 ++++++++++ 4 files changed, 68 insertions(+), 1 deletion(-) create mode 100644 common/data_refinery_common/migrations/0071_auto_20220901_1653.py create mode 100644 common/data_refinery_common/models/accession.py diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index d024704da..b651ce24a 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -22,7 +22,7 @@ repos: - id: isort - repo: https://github.com/psf/black - rev: 19.10b0 + rev: 22.3.0 hooks: - id: black args: [--line-length=100] diff --git a/common/data_refinery_common/migrations/0071_auto_20220901_1653.py b/common/data_refinery_common/migrations/0071_auto_20220901_1653.py new file mode 100644 index 000000000..c7d3b0b63 --- /dev/null +++ b/common/data_refinery_common/migrations/0071_auto_20220901_1653.py @@ -0,0 +1,44 @@ +# Generated by Django 3.2.7 on 2022-09-01 16:53 + +from django.db import migrations, models + + +class Migration(migrations.Migration): + + dependencies = [ + ("data_refinery_common", "0070_auto_20211208_2118"), + ] + + operations = [ + migrations.CreateModel( + name="Accession", + fields=[ + ( + "id", + models.AutoField( + auto_created=True, + primary_key=True, + serialize=False, + verbose_name="ID", + ), + ), + ("code", models.TextField()), + ("created_at", models.DateTimeField(auto_now_add=True)), + ("last_modified_at", models.DateTimeField(auto_now=True)), + ("organism", models.TextField()), + ("published_date", models.DateTimeField()), + ("sample_count", models.PositiveIntegerField(default=0)), + ("source", models.TextField()), + ("technology", models.TextField()), + ], + options={ + "db_table": "accessions", + }, + ), + migrations.AddConstraint( + model_name="accession", + constraint=models.UniqueConstraint( + fields=("code", "source", "technology"), name="unique_accession" + ), + ), + ] diff --git a/common/data_refinery_common/models/__init__.py b/common/data_refinery_common/models/__init__.py index 39abe7ee3..8e9564153 100644 --- a/common/data_refinery_common/models/__init__.py +++ b/common/data_refinery_common/models/__init__.py @@ -1,3 +1,4 @@ +from data_refinery_common.models.accession import Accession # noqa from data_refinery_common.models.api_token import APIToken # noqa from data_refinery_common.models.associations.compendium_result_organism_association import ( # noqa CompendiumResultOrganismAssociation, diff --git a/common/data_refinery_common/models/accession.py b/common/data_refinery_common/models/accession.py new file mode 100644 index 000000000..dc93cfd88 --- /dev/null +++ b/common/data_refinery_common/models/accession.py @@ -0,0 +1,22 @@ +from django.db import models + + +class Accession(models.Model): + """Accession model.""" + + class Meta: + constraints = ( + models.UniqueConstraint( + fields=("code", "source", "technology"), name="unique_accession" + ), + ) + db_table = "accessions" + + code = models.TextField() + created_at = models.DateTimeField(auto_now_add=True) + last_modified_at = models.DateTimeField(auto_now=True) + organism = models.TextField() + published_date = models.DateTimeField() + sample_count = models.PositiveIntegerField(default=0) + source = models.TextField() + technology = models.TextField() From 8f7f51693d53f75a8336f66c340383d58adaee78 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 1 Sep 2022 11:37:53 -0700 Subject: [PATCH 011/220] Run black. --- api/data_refinery_api/middleware.py | 10 +- api/data_refinery_api/settings.py | 16 ++- .../test/test_api_general.py | 4 +- api/data_refinery_api/test/test_compendia.py | 3 +- api/data_refinery_api/test/test_dataset.py | 19 ++- .../test/test_dataset_stats.py | 2 +- api/data_refinery_api/test/test_search.py | 6 +- api/data_refinery_api/urls.py | 14 ++- api/data_refinery_api/views/api_token.py | 4 +- api/data_refinery_api/views/dataset.py | 60 +++++++--- api/data_refinery_api/views/experiment.py | 6 +- .../views/experiment_document.py | 5 +- .../views/jobs/downloader_job.py | 2 +- .../views/jobs/processor_job.py | 2 +- .../views/jobs/survey_job.py | 2 +- api/data_refinery_api/views/stats.py | 4 +- common/data_refinery_common/job_management.py | 6 +- common/data_refinery_common/message_queue.py | 6 +- .../migrations/0001_initial.py | 112 +++++++++++++----- .../migrations/0005_auto_20181030_1955.py | 8 +- .../migrations/0006_auto_20181102_1526.py | 4 +- .../migrations/0008_auto_20190104_2135.py | 7 +- .../migrations/0009_es_cache.py | 4 +- .../migrations/0015_dataset_email_ccdl_ok.py | 4 +- .../0018_downloaderjob_ram_amount.py | 4 +- .../migrations/0019_sample_is_blacklisted.py | 4 +- .../migrations/0027_organism_qn_target.py | 2 +- .../0030_update_sample_manufacturer.py | 2 +- .../migrations/0032_surveyedaccession.py | 4 +- .../migrations/0034_cdfcorrectedaccession.py | 4 +- .../0038_paginate_default_svd_algorithm.py | 8 +- .../migrations/0039_auto_20190925_1338.py | 5 +- .../0042_remove_experiment_organism_names.py | 5 +- .../migrations/0043_auto_20191108_2144.py | 9 +- .../migrations/0044_compendium_results.py | 2 +- .../migrations/0045_svd_algorithm.py | 2 +- .../0046_compendium_version_number.py | 2 +- .../migrations/0047_clean_compendia_files.py | 2 +- .../migrations/0048_processorjob_abort.py | 6 +- .../0050_remove_organismindex_s3_url.py | 5 +- .../0051_remove_dataset_email_sent.py | 5 +- ...52_remove_invalid_tximport_associations.py | 4 +- .../migrations/0054_datasetannotation.py | 5 +- .../migrations/0056_auto_20200529_1230.py | 8 +- .../migrations/0059_auto_20200819_1632.py | 12 +- .../migrations/0060_auto_20200828_1650.py | 4 +- .../0061_transcriptome_database_name.py | 2 +- .../migrations/0064_auto_20210225_1622.py | 5 +- .../migrations/0065_auto_20210324_1948.py | 36 ++++-- .../migrations/0066_auto_20210611_1900.py | 16 ++- .../migrations/0067_dataset_notify_me.py | 4 +- .../migrations/0068_auto_20210729_1610.py | 4 +- ...version_computedfile_compendium_version.py | 4 +- .../data_refinery_common/models/api_token.py | 4 +- .../models/base_models.py | 2 +- .../models/command_progress.py | 4 +- .../models/compendium_result.py | 6 +- .../models/computational_result.py | 13 +- .../models/computational_result_annotation.py | 4 +- .../models/computed_file.py | 36 +++--- .../models/contributions.py | 2 +- .../models/dataset_annotation.py | 6 +- .../data_refinery_common/models/documents.py | 6 +- .../models/experiment_annotation.py | 4 +- .../models/jobs/downloader_job.py | 2 +- .../models/jobs/processor_job.py | 6 +- .../models/jobs/survey_job.py | 6 +- .../data_refinery_common/models/organism.py | 4 +- .../models/organism_index.py | 6 +- .../models/original_file.py | 31 ++--- .../models/sample_annotation.py | 4 +- .../models/test_ontology_term.py | 6 +- .../models/test_organisms.py | 3 +- .../runtests/settings.py | 7 +- common/data_refinery_common/settings.py | 16 ++- common/data_refinery_common/signals.py | 3 +- common/data_refinery_common/test_utils.py | 2 +- common/data_refinery_common/utils.py | 22 ++-- .../foreman/job_control.py | 2 +- .../foreman/job_requeuing.py | 4 +- .../commands/check_computed_files.py | 2 +- .../management/commands/correct_affy_cdfs.py | 3 +- .../management/commands/create_compendia.py | 10 +- .../create_missing_downloader_jobs.py | 2 +- .../management/commands/create_quantpendia.py | 5 +- .../management/commands/fix_compendia.py | 5 +- .../commands/generate_dataset_report.py | 4 +- .../import_external_sample_keywords.py | 4 +- .../management/commands/import_ontology.py | 4 +- .../commands/mark_samples_unprocessable.py | 22 ++-- .../management/commands/organism_shepherd.py | 3 +- .../management/commands/retry_samples.py | 4 +- .../management/commands/run_tximport.py | 6 +- .../foreman/management/commands/stop_jobs.py | 4 +- .../commands/test_rerun_salmon_old_samples.py | 2 +- .../management/commands/test_retry_samples.py | 5 +- .../commands/update_experiment_metadata.py | 7 +- .../commands/update_sample_metadata.py | 3 +- foreman/data_refinery_foreman/settings.py | 16 ++- .../surveyor/external_source.py | 3 +- .../data_refinery_foreman/surveyor/harmony.py | 6 +- foreman/data_refinery_foreman/surveyor/sra.py | 2 +- .../surveyor/test_array_express.py | 3 +- .../surveyor/test_geo.py | 9 +- .../surveyor/test_harmony.py | 4 +- .../surveyor/test_sra.py | 12 +- .../surveyor/test_transcriptome_index.py | 8 +- .../surveyor/transcriptome_index.py | 7 +- .../data_refinery_foreman/surveyor/utils.py | 2 +- foreman/data_refinery_foreman/test_runner.py | 6 +- scripts/kill_all_jobs.py | 4 +- scripts/run_full_pipeline.py | 3 +- .../downloaders/array_express.py | 7 +- .../data_refinery_workers/downloaders/geo.py | 25 ++-- .../data_refinery_workers/downloaders/sra.py | 5 +- .../downloaders/test_geo.py | 7 +- .../downloaders/transcriptome_index.py | 2 +- .../processors/create_quantpendia.py | 4 +- .../processors/illumina.py | 13 +- .../processors/janitor.py | 2 +- .../management/commands/fetch_compendia.py | 3 +- .../management/commands/qn_dispatcher.py | 2 +- .../remove_invalid_computational_results.py | 2 +- .../management/commands/run_tximport.py | 3 +- .../data_refinery_workers/processors/no_op.py | 19 +-- .../processors/smasher.py | 28 ++++- .../processors/test_illumina.py | 25 +++- .../processors/test_janitor.py | 2 +- .../processors/test_salmon.py | 6 +- .../processors/test_transcriptome_index.py | 5 +- .../processors/tximport.py | 5 +- 131 files changed, 670 insertions(+), 371 deletions(-) diff --git a/api/data_refinery_api/middleware.py b/api/data_refinery_api/middleware.py index a9bd5c503..988dfae59 100644 --- a/api/data_refinery_api/middleware.py +++ b/api/data_refinery_api/middleware.py @@ -25,7 +25,10 @@ def process_response(self, request, response): # create data representation data = client.get_data_from_request(request) data.update( - {"level": logging.WARN, "logger": "BadRequestMiddleware",} + { + "level": logging.WARN, + "logger": "BadRequestMiddleware", + } ) client.captureMessage(message=message, data=data) @@ -40,7 +43,10 @@ def process_exception(self, request, exception): data = client.get_data_from_request(request) data.update( - {"level": logging.WARN, "logger": "BadRequestMiddleware",} + { + "level": logging.WARN, + "logger": "BadRequestMiddleware", + } ) client.captureMessage(message=str(exception), data=data) diff --git a/api/data_refinery_api/settings.py b/api/data_refinery_api/settings.py index 7d2ea00f5..e185a699b 100644 --- a/api/data_refinery_api/settings.py +++ b/api/data_refinery_api/settings.py @@ -121,10 +121,18 @@ # https://docs.djangoproject.com/en/1.10/ref/settings/#auth-password-validators AUTH_PASSWORD_VALIDATORS = [ - {"NAME": ("django.contrib.auth.password_validation" + ".UserAttributeSimilarityValidator"),}, - {"NAME": ("django.contrib.auth.password_validation." + "MinimumLengthValidator"),}, - {"NAME": ("django.contrib.auth.password_validation" + ".CommonPasswordValidator"),}, - {"NAME": ("django.contrib.auth.password_validation" + ".NumericPasswordValidator"),}, + { + "NAME": ("django.contrib.auth.password_validation" + ".UserAttributeSimilarityValidator"), + }, + { + "NAME": ("django.contrib.auth.password_validation." + "MinimumLengthValidator"), + }, + { + "NAME": ("django.contrib.auth.password_validation" + ".CommonPasswordValidator"), + }, + { + "NAME": ("django.contrib.auth.password_validation" + ".NumericPasswordValidator"), + }, ] diff --git a/api/data_refinery_api/test/test_api_general.py b/api/data_refinery_api/test/test_api_general.py index 7b48cc803..49f7e7331 100644 --- a/api/data_refinery_api/test/test_api_general.py +++ b/api/data_refinery_api/test/test_api_general.py @@ -205,7 +205,7 @@ def setUp(self): return def tearDown(self): - """ Good bye """ + """Good bye""" cache.clear() Experiment.objects.all().delete() ExperimentAnnotation.objects.all().delete() @@ -483,7 +483,7 @@ def test_fetching_organism_index(self): self.assertEqual(response.status_code, 404) def test_processed_samples_only(self): - """ Don't return unprocessed samples """ + """Don't return unprocessed samples""" experiment = Experiment() experiment.accession_code = "GSX12345" experiment.is_public = True diff --git a/api/data_refinery_api/test/test_compendia.py b/api/data_refinery_api/test/test_compendia.py index 0cfb2c19c..ddd9677e9 100644 --- a/api/data_refinery_api/test/test_compendia.py +++ b/api/data_refinery_api/test/test_compendia.py @@ -124,7 +124,8 @@ def test_organism_has_compendia(self): self.assertEqual(2, len(response_json)) response = self.client.get( - reverse("organisms", kwargs={"version": API_VERSION}), {"has_compendia": True}, + reverse("organisms", kwargs={"version": API_VERSION}), + {"has_compendia": True}, ) response_json = response.json()["results"] self.assertEqual(1, len(response_json)) diff --git a/api/data_refinery_api/test/test_dataset.py b/api/data_refinery_api/test/test_dataset.py index e360c8407..c0293db96 100644 --- a/api/data_refinery_api/test/test_dataset.py +++ b/api/data_refinery_api/test/test_dataset.py @@ -205,7 +205,12 @@ def test_create_dataset_fails_without_email(self): # Good, except for empty email. jdata = json.dumps( - {"start": True, "data": {"GSE123": ["789"]}, "token_id": token_id, "email_address": "",} + { + "start": True, + "data": {"GSE123": ["789"]}, + "token_id": token_id, + "email_address": "", + } ) response = self.client.post( reverse("create_dataset", kwargs={"version": API_VERSION}), @@ -328,7 +333,8 @@ def test_dataset_adding_non_downloadable_samples_fails(self): ) self.assertEqual(response.status_code, 400) self.assertIn( - "Non-downloadable sample(s) in dataset", response.json()["message"], + "Non-downloadable sample(s) in dataset", + response.json()["message"], ) self.assertEqual(response.json()["details"], ["456"]) cache.clear() @@ -341,7 +347,8 @@ def test_dataset_adding_non_downloadable_samples_fails(self): content_type="application/json", ) self.assertIn( - "Sample(s) in dataset do not exist on refine", response.json()["message"], + "Sample(s) in dataset do not exist on refine", + response.json()["message"], ) self.assertEqual(response.status_code, 400) cache.clear() @@ -374,7 +381,8 @@ def test_dataset_adding_non_downloadable_samples_fails(self): ) self.assertEqual(response.status_code, 400) self.assertIn( - "Sample(s) in dataset are missing quant.sf files", response.json()["message"], + "Sample(s) in dataset are missing quant.sf files", + response.json()["message"], ) self.assertEqual(response.json()["details"], ["456"]) cache.clear() @@ -394,7 +402,8 @@ def test_dataset_adding_non_downloadable_samples_fails(self): ) self.assertEqual(response.status_code, 400) self.assertIn( - "Experiment(s) in dataset have zero downloadable samples", response.json()["message"], + "Experiment(s) in dataset have zero downloadable samples", + response.json()["message"], ) self.assertEqual(response.json()["details"], ["GSE123"]) cache.clear() diff --git a/api/data_refinery_api/test/test_dataset_stats.py b/api/data_refinery_api/test/test_dataset_stats.py index 6c14181d8..f29971b68 100644 --- a/api/data_refinery_api/test/test_dataset_stats.py +++ b/api/data_refinery_api/test/test_dataset_stats.py @@ -20,7 +20,7 @@ def setUp(self): self.homo_sapiens.save() def test_dataset_stats(self): - """ Test the dataset stats endpoint """ + """Test the dataset stats endpoint""" gallus_gallus = Organism(name="GALLUS_GALLUS", taxonomy_id=9031, is_scientific_name=True) gallus_gallus.save() diff --git a/api/data_refinery_api/test/test_search.py b/api/data_refinery_api/test/test_search.py index a7a5c05b6..d09917b48 100644 --- a/api/data_refinery_api/test/test_search.py +++ b/api/data_refinery_api/test/test_search.py @@ -66,7 +66,11 @@ def setUpClass(cls): sample.accession_code = "123" sample.save() - organism = Organism(name=ECOLI_STRAIN_NAME, taxonomy_id=879462, is_scientific_name=True,) + organism = Organism( + name=ECOLI_STRAIN_NAME, + taxonomy_id=879462, + is_scientific_name=True, + ) organism.save() sample = Sample() diff --git a/api/data_refinery_api/urls.py b/api/data_refinery_api/urls.py index ff30ee2c6..751e85929 100644 --- a/api/data_refinery_api/urls.py +++ b/api/data_refinery_api/urls.py @@ -59,12 +59,22 @@ def handle404error(request, exception): if not match(r"^/v[1-9]/.*", request.path): message = "refine.bio API resources are only available through versioned requests." - return JsonResponse({"message": message, "docs": url, "status_code": 404,}, status=404) + return JsonResponse( + { + "message": message, + "docs": url, + "status_code": 404, + }, + status=404, + ) def handle500error(request): return JsonResponse( - {"message": "A server error occured. This has been reported.", "status_code": 500,}, + { + "message": "A server error occured. This has been reported.", + "status_code": 500, + }, status=500, ) diff --git a/api/data_refinery_api/views/api_token.py b/api/data_refinery_api/views/api_token.py index 34e73aef9..71da7aabc 100644 --- a/api/data_refinery_api/views/api_token.py +++ b/api/data_refinery_api/views/api_token.py @@ -49,7 +49,9 @@ class Meta: ) @method_decorator( name="retrieve", - decorator=swagger_auto_schema(operation_description="Return details about a specific token.",), + decorator=swagger_auto_schema( + operation_description="Return details about a specific token.", + ), ) @method_decorator( name="update", diff --git a/api/data_refinery_api/views/dataset.py b/api/data_refinery_api/views/dataset.py index cf65489f4..c3699101c 100644 --- a/api/data_refinery_api/views/dataset.py +++ b/api/data_refinery_api/views/dataset.py @@ -221,24 +221,54 @@ class Meta: "worker_version", ) extra_kwargs = { - "data": {"required": True,}, - "id": {"read_only": True,}, - "is_processing": {"read_only": True,}, - "is_processed": {"read_only": True,}, - "is_available": {"read_only": True,}, + "data": { + "required": True, + }, + "id": { + "read_only": True, + }, + "is_processing": { + "read_only": True, + }, + "is_processed": { + "read_only": True, + }, + "is_available": { + "read_only": True, + }, "email_address": {"required": False, "write_only": True}, "email_ccdl_ok": {"required": False, "write_only": True}, "notify_me": {"required": False, "write_only": True}, - "expires_on": {"read_only": True,}, - "s3_bucket": {"read_only": True,}, - "s3_key": {"read_only": True,}, - "success": {"read_only": True,}, - "failure_reason": {"read_only": True,}, - "created_at": {"read_only": True,}, - "last_modified": {"read_only": True,}, - "size_in_bytes": {"read_only": True,}, - "sha1": {"read_only": True,}, - "download_url": {"read_only": True,}, + "expires_on": { + "read_only": True, + }, + "s3_bucket": { + "read_only": True, + }, + "s3_key": { + "read_only": True, + }, + "success": { + "read_only": True, + }, + "failure_reason": { + "read_only": True, + }, + "created_at": { + "read_only": True, + }, + "last_modified": { + "read_only": True, + }, + "size_in_bytes": { + "read_only": True, + }, + "sha1": { + "read_only": True, + }, + "download_url": { + "read_only": True, + }, "worker_version": { "read_only": True, "help_text": "Returns the latest version of refine.bio that was used to build this dataset.", diff --git a/api/data_refinery_api/views/experiment.py b/api/data_refinery_api/views/experiment.py index d7148d509..6ed81f2e8 100644 --- a/api/data_refinery_api/views/experiment.py +++ b/api/data_refinery_api/views/experiment.py @@ -66,7 +66,7 @@ class Meta: @staticmethod def setup_eager_loading(queryset): - """ Perform necessary eager loading of data. """ + """Perform necessary eager loading of data.""" queryset = queryset.prefetch_related("samples").prefetch_related("organisms") # Multiple count annotations @@ -117,7 +117,7 @@ class Meta: class ExperimentListView(generics.ListAPIView): - """ Paginated list of all experiments. Advanced filtering can be done with the `/search` endpoint. """ + """Paginated list of all experiments. Advanced filtering can be done with the `/search` endpoint.""" model = Experiment queryset = Experiment.public_objects.all() @@ -152,7 +152,7 @@ def get_queryset(self): class ExperimentDetailView(generics.RetrieveAPIView): - """ Retrieve details for an experiment given it's accession code """ + """Retrieve details for an experiment given it's accession code""" lookup_field = "accession_code" queryset = Experiment.public_objects.all() diff --git a/api/data_refinery_api/views/experiment_document.py b/api/data_refinery_api/views/experiment_document.py index e1780c791..b1e177a56 100644 --- a/api/data_refinery_api/views/experiment_document.py +++ b/api/data_refinery_api/views/experiment_document.py @@ -276,7 +276,10 @@ class ExperimentDocumentView(DocumentViewSet): # Define filtering fields filter_fields = { - "id": {"field": "_id", "lookups": [LOOKUP_FILTER_RANGE, LOOKUP_QUERY_IN],}, + "id": { + "field": "_id", + "lookups": [LOOKUP_FILTER_RANGE, LOOKUP_QUERY_IN], + }, "technology": "technology", "has_publication": "has_publication", "accession_code": "accession_code", diff --git a/api/data_refinery_api/views/jobs/downloader_job.py b/api/data_refinery_api/views/jobs/downloader_job.py index 730de72e5..33419b54d 100644 --- a/api/data_refinery_api/views/jobs/downloader_job.py +++ b/api/data_refinery_api/views/jobs/downloader_job.py @@ -120,7 +120,7 @@ def get_queryset(self): class DownloaderJobDetailView(generics.RetrieveAPIView): - """ Retrieves a DownloaderJob by ID """ + """Retrieves a DownloaderJob by ID""" lookup_field = "id" model = DownloaderJob diff --git a/api/data_refinery_api/views/jobs/processor_job.py b/api/data_refinery_api/views/jobs/processor_job.py index a7e590e5a..1d86f9cf0 100644 --- a/api/data_refinery_api/views/jobs/processor_job.py +++ b/api/data_refinery_api/views/jobs/processor_job.py @@ -121,7 +121,7 @@ def get_queryset(self): class ProcessorJobDetailView(generics.RetrieveAPIView): - """ Retrieves a ProcessorJob by ID """ + """Retrieves a ProcessorJob by ID""" lookup_field = "id" model = ProcessorJob diff --git a/api/data_refinery_api/views/jobs/survey_job.py b/api/data_refinery_api/views/jobs/survey_job.py index 80d04afdd..b3f492932 100644 --- a/api/data_refinery_api/views/jobs/survey_job.py +++ b/api/data_refinery_api/views/jobs/survey_job.py @@ -90,7 +90,7 @@ def get_queryset(self): class SurveyJobDetailView(generics.RetrieveAPIView): - """ Retrieves a SurveyJob by ID """ + """Retrieves a SurveyJob by ID""" lookup_field = "id" model = SurveyJob diff --git a/api/data_refinery_api/views/stats.py b/api/data_refinery_api/views/stats.py index 19ac6360a..25c7c3fdf 100644 --- a/api/data_refinery_api/views/stats.py +++ b/api/data_refinery_api/views/stats.py @@ -116,7 +116,9 @@ def get_jobs_in_queue(batch_job_queue: str) -> list: while "nextToken" in list_jobs_dict and list_jobs_dict["nextToken"]: list_jobs_dict = batch.list_jobs( - jobQueue=batch_job_queue, jobStatus=status, nextToken=list_jobs_dict["nextToken"], + jobQueue=batch_job_queue, + jobStatus=status, + nextToken=list_jobs_dict["nextToken"], ) jobs.extend(list_jobs_dict["jobSummaryList"]) diff --git a/common/data_refinery_common/job_management.py b/common/data_refinery_common/job_management.py index 4076696fd..789c0e7f5 100644 --- a/common/data_refinery_common/job_management.py +++ b/common/data_refinery_common/job_management.py @@ -168,7 +168,8 @@ def create_downloader_job( def create_processor_jobs_for_original_files( - original_files: List[OriginalFile], downloader_job: DownloaderJob = None, + original_files: List[OriginalFile], + downloader_job: DownloaderJob = None, ): """ Creates one processor job for each original file given. @@ -178,7 +179,8 @@ def create_processor_jobs_for_original_files( def create_processor_job_for_original_files( - original_files: List[OriginalFile], downloader_job: DownloaderJob = None, + original_files: List[OriginalFile], + downloader_job: DownloaderJob = None, ): """ Create a processor job and queue a processor task for sample related to an experiment. diff --git a/common/data_refinery_common/message_queue.py b/common/data_refinery_common/message_queue.py index 45d6fcabb..d92f8ec05 100644 --- a/common/data_refinery_common/message_queue.py +++ b/common/data_refinery_common/message_queue.py @@ -111,8 +111,7 @@ def get_downloader_job_queue_depth(job_queue_name): def get_capacity_for_jobs() -> bool: - """Returns how many jobs the system has remaining capacity for. - """ + """Returns how many jobs the system has remaining capacity for.""" num_job_queues = len(settings.AWS_BATCH_QUEUE_WORKERS_NAMES) MAX_JOBS_PER_NODE = int(get_env_variable("MAX_JOBS_PER_NODE")) # Maximum number of total jobs running at a time. @@ -163,8 +162,7 @@ def increment_downloader_job_queue_depth(job_queue_name): def get_batch_queue_for_downloader_job(): - """Logic for distributing downloader jobs across queues. - """ + """Logic for distributing downloader jobs across queues.""" job_queue_depths = get_job_queue_depths()["downloader_jobs"] for job_queue in settings.AWS_BATCH_QUEUE_WORKERS_NAMES: if job_queue_depths[job_queue] <= settings.MAX_DOWNLOADER_JOBS_PER_NODE: diff --git a/common/data_refinery_common/migrations/0001_initial.py b/common/data_refinery_common/migrations/0001_initial.py index ffcad7927..0a591f807 100644 --- a/common/data_refinery_common/migrations/0001_initial.py +++ b/common/data_refinery_common/migrations/0001_initial.py @@ -59,7 +59,10 @@ class Migration(migrations.Migration): ), ("last_modified", models.DateTimeField(default=django.utils.timezone.now)), ], - options={"db_table": "computational_results", "base_manager_name": "public_objects",}, + options={ + "db_table": "computational_results", + "base_manager_name": "public_objects", + }, managers=[ ("objects", django.db.models.manager.Manager()), ("public_objects", django.db.models.manager.Manager()), @@ -131,7 +134,10 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "computed_files", "get_latest_by": "created_at",}, + options={ + "db_table": "computed_files", + "get_latest_by": "created_at", + }, ), migrations.CreateModel( name="Dataset", @@ -213,7 +219,9 @@ class Migration(migrations.Migration): ), ("last_modified", models.DateTimeField(default=django.utils.timezone.now)), ], - options={"db_table": "downloader_jobs",}, + options={ + "db_table": "downloader_jobs", + }, ), migrations.CreateModel( name="DownloaderJobOriginalFileAssociation", @@ -232,7 +240,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "downloaderjob_originalfile_associations",}, + options={ + "db_table": "downloaderjob_originalfile_associations", + }, ), migrations.CreateModel( name="Experiment", @@ -273,7 +283,10 @@ class Migration(migrations.Migration): ), ("last_modified", models.DateTimeField(default=django.utils.timezone.now)), ], - options={"db_table": "experiments", "base_manager_name": "public_objects",}, + options={ + "db_table": "experiments", + "base_manager_name": "public_objects", + }, managers=[ ("objects", django.db.models.manager.Manager()), ("public_objects", django.db.models.manager.Manager()), @@ -304,7 +317,10 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "experiment_annotations", "base_manager_name": "public_objects",}, + options={ + "db_table": "experiment_annotations", + "base_manager_name": "public_objects", + }, managers=[ ("objects", django.db.models.manager.Manager()), ("public_objects", django.db.models.manager.Manager()), @@ -327,7 +343,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "experiment_organism_associations",}, + options={ + "db_table": "experiment_organism_associations", + }, ), migrations.CreateModel( name="ExperimentSampleAssociation", @@ -346,7 +364,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "experiment_sample_associations",}, + options={ + "db_table": "experiment_sample_associations", + }, ), migrations.CreateModel( name="Organism", @@ -366,7 +386,9 @@ class Migration(migrations.Migration): ), ("last_modified", models.DateTimeField(default=django.utils.timezone.now)), ], - options={"db_table": "organisms",}, + options={ + "db_table": "organisms", + }, ), migrations.CreateModel( name="OrganismIndex", @@ -407,7 +429,10 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "organism_index", "base_manager_name": "public_objects",}, + options={ + "db_table": "organism_index", + "base_manager_name": "public_objects", + }, managers=[ ("objects", django.db.models.manager.Manager()), ("public_objects", django.db.models.manager.Manager()), @@ -440,7 +465,9 @@ class Migration(migrations.Migration): ), ("last_modified", models.DateTimeField(default=django.utils.timezone.now)), ], - options={"db_table": "original_files",}, + options={ + "db_table": "original_files", + }, ), migrations.CreateModel( name="OriginalFileSampleAssociation", @@ -459,7 +486,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "original_file_sample_associations",}, + options={ + "db_table": "original_file_sample_associations", + }, ), migrations.CreateModel( name="Pipeline", @@ -478,7 +507,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "pipelines",}, + options={ + "db_table": "pipelines", + }, ), migrations.CreateModel( name="Processor", @@ -494,7 +525,9 @@ class Migration(migrations.Migration): ("docker_image", models.CharField(max_length=255)), ("environment", django.contrib.postgres.fields.jsonb.JSONField(default={})), ], - options={"db_table": "processors",}, + options={ + "db_table": "processors", + }, ), migrations.CreateModel( name="ProcessorJob", @@ -524,7 +557,9 @@ class Migration(migrations.Migration): ), ("last_modified", models.DateTimeField(default=django.utils.timezone.now)), ], - options={"db_table": "processor_jobs",}, + options={ + "db_table": "processor_jobs", + }, ), migrations.CreateModel( name="ProcessorJobDatasetAssociation", @@ -550,7 +585,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "processorjob_dataset_associations",}, + options={ + "db_table": "processorjob_dataset_associations", + }, ), migrations.CreateModel( name="ProcessorJobOriginalFileAssociation", @@ -576,7 +613,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "processorjob_originalfile_associations",}, + options={ + "db_table": "processorjob_originalfile_associations", + }, ), migrations.CreateModel( name="Sample", @@ -659,7 +698,10 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "sample_annotations", "base_manager_name": "public_objects",}, + options={ + "db_table": "sample_annotations", + "base_manager_name": "public_objects", + }, managers=[ ("objects", django.db.models.manager.Manager()), ("public_objects", django.db.models.manager.Manager()), @@ -689,7 +731,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "sample_computed_file_associations",}, + options={ + "db_table": "sample_computed_file_associations", + }, ), migrations.CreateModel( name="SampleResultAssociation", @@ -715,7 +759,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "sample_result_associations",}, + options={ + "db_table": "sample_result_associations", + }, ), migrations.CreateModel( name="SurveyJob", @@ -738,7 +784,9 @@ class Migration(migrations.Migration): ), ("last_modified", models.DateTimeField(default=django.utils.timezone.now)), ], - options={"db_table": "survey_jobs",}, + options={ + "db_table": "survey_jobs", + }, ), migrations.CreateModel( name="SurveyJobKeyValue", @@ -759,7 +807,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "survey_job_key_values",}, + options={ + "db_table": "survey_job_key_values", + }, ), migrations.AddField( model_name="sample", @@ -821,7 +871,8 @@ class Migration(migrations.Migration): ), ), migrations.AlterUniqueTogether( - name="processor", unique_together={("name", "version", "docker_image", "environment")}, + name="processor", + unique_together={("name", "version", "docker_image", "environment")}, ), migrations.AddField( model_name="originalfilesampleassociation", @@ -921,23 +972,28 @@ class Migration(migrations.Migration): ), ), migrations.AlterUniqueTogether( - name="sampleresultassociation", unique_together={("result", "sample")}, + name="sampleresultassociation", + unique_together={("result", "sample")}, ), migrations.AlterUniqueTogether( - name="samplecomputedfileassociation", unique_together={("sample", "computed_file")}, + name="samplecomputedfileassociation", + unique_together={("sample", "computed_file")}, ), migrations.AlterUniqueTogether( name="processorjoboriginalfileassociation", unique_together={("processor_job", "original_file")}, ), migrations.AlterUniqueTogether( - name="originalfilesampleassociation", unique_together={("original_file", "sample")}, + name="originalfilesampleassociation", + unique_together={("original_file", "sample")}, ), migrations.AlterUniqueTogether( - name="experimentsampleassociation", unique_together={("experiment", "sample")}, + name="experimentsampleassociation", + unique_together={("experiment", "sample")}, ), migrations.AlterUniqueTogether( - name="experimentorganismassociation", unique_together={("experiment", "organism")}, + name="experimentorganismassociation", + unique_together={("experiment", "organism")}, ), migrations.AlterUniqueTogether( name="downloaderjoboriginalfileassociation", diff --git a/common/data_refinery_common/migrations/0005_auto_20181030_1955.py b/common/data_refinery_common/migrations/0005_auto_20181030_1955.py index 9f20949e5..2682b7ad6 100644 --- a/common/data_refinery_common/migrations/0005_auto_20181030_1955.py +++ b/common/data_refinery_common/migrations/0005_auto_20181030_1955.py @@ -11,9 +11,13 @@ class Migration(migrations.Migration): operations = [ migrations.AddField( - model_name="surveyjob", name="num_retries", field=models.IntegerField(default=0), + model_name="surveyjob", + name="num_retries", + field=models.IntegerField(default=0), ), migrations.AddField( - model_name="surveyjob", name="retried", field=models.BooleanField(default=False), + model_name="surveyjob", + name="retried", + field=models.BooleanField(default=False), ), ] diff --git a/common/data_refinery_common/migrations/0006_auto_20181102_1526.py b/common/data_refinery_common/migrations/0006_auto_20181102_1526.py index e3ba4ffda..0192cc83b 100644 --- a/common/data_refinery_common/migrations/0006_auto_20181102_1526.py +++ b/common/data_refinery_common/migrations/0006_auto_20181102_1526.py @@ -16,6 +16,8 @@ class Migration(migrations.Migration): field=models.CharField(max_length=256, null=True), ), migrations.AddField( - model_name="surveyjob", name="ram_amount", field=models.IntegerField(default=256), + model_name="surveyjob", + name="ram_amount", + field=models.IntegerField(default=256), ), ] diff --git a/common/data_refinery_common/migrations/0008_auto_20190104_2135.py b/common/data_refinery_common/migrations/0008_auto_20190104_2135.py index c4f2daa9f..901a30443 100644 --- a/common/data_refinery_common/migrations/0008_auto_20190104_2135.py +++ b/common/data_refinery_common/migrations/0008_auto_20190104_2135.py @@ -28,7 +28,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "experiment_result_associations",}, + options={ + "db_table": "experiment_result_associations", + }, ), migrations.AddField( model_name="computationalresult", @@ -47,6 +49,7 @@ class Migration(migrations.Migration): ), ), migrations.AlterUniqueTogether( - name="experimentresultassociation", unique_together={("result", "experiment")}, + name="experimentresultassociation", + unique_together={("result", "experiment")}, ), ] diff --git a/common/data_refinery_common/migrations/0009_es_cache.py b/common/data_refinery_common/migrations/0009_es_cache.py index 9fbbc25bc..2dc7b1869 100644 --- a/common/data_refinery_common/migrations/0009_es_cache.py +++ b/common/data_refinery_common/migrations/0009_es_cache.py @@ -67,7 +67,9 @@ class Migration(migrations.Migration): field=models.IntegerField(default=0), ), migrations.AddField( - model_name="experiment", name="num_total_samples", field=models.IntegerField(default=0), + model_name="experiment", + name="num_total_samples", + field=models.IntegerField(default=0), ), migrations.AddField( model_name="experiment", diff --git a/common/data_refinery_common/migrations/0015_dataset_email_ccdl_ok.py b/common/data_refinery_common/migrations/0015_dataset_email_ccdl_ok.py index a7e1fd461..723ceaf51 100644 --- a/common/data_refinery_common/migrations/0015_dataset_email_ccdl_ok.py +++ b/common/data_refinery_common/migrations/0015_dataset_email_ccdl_ok.py @@ -11,6 +11,8 @@ class Migration(migrations.Migration): operations = [ migrations.AddField( - model_name="dataset", name="email_ccdl_ok", field=models.BooleanField(default=False), + model_name="dataset", + name="email_ccdl_ok", + field=models.BooleanField(default=False), ), ] diff --git a/common/data_refinery_common/migrations/0018_downloaderjob_ram_amount.py b/common/data_refinery_common/migrations/0018_downloaderjob_ram_amount.py index ad4829039..9c235e0e0 100644 --- a/common/data_refinery_common/migrations/0018_downloaderjob_ram_amount.py +++ b/common/data_refinery_common/migrations/0018_downloaderjob_ram_amount.py @@ -11,6 +11,8 @@ class Migration(migrations.Migration): operations = [ migrations.AddField( - model_name="downloaderjob", name="ram_amount", field=models.IntegerField(default=1024), + model_name="downloaderjob", + name="ram_amount", + field=models.IntegerField(default=1024), ), ] diff --git a/common/data_refinery_common/migrations/0019_sample_is_blacklisted.py b/common/data_refinery_common/migrations/0019_sample_is_blacklisted.py index 3193e5da4..8decc97d2 100644 --- a/common/data_refinery_common/migrations/0019_sample_is_blacklisted.py +++ b/common/data_refinery_common/migrations/0019_sample_is_blacklisted.py @@ -33,7 +33,9 @@ class Migration(migrations.Migration): operations = [ migrations.AddField( - model_name="sample", name="is_blacklisted", field=models.BooleanField(default=False), + model_name="sample", + name="is_blacklisted", + field=models.BooleanField(default=False), ), migrations.RunPython(update_blacklisted_samples), ] diff --git a/common/data_refinery_common/migrations/0027_organism_qn_target.py b/common/data_refinery_common/migrations/0027_organism_qn_target.py index 58b4a9644..d4804d815 100644 --- a/common/data_refinery_common/migrations/0027_organism_qn_target.py +++ b/common/data_refinery_common/migrations/0027_organism_qn_target.py @@ -5,7 +5,7 @@ def update_organisms_qn_targets(apps, schema_editor): - """ Set the latest qn_target to each organism. """ + """Set the latest qn_target to each organism.""" Organism = apps.get_model("data_refinery_common", "Organism") ComputationalResultAnnotation = apps.get_model( "data_refinery_common", "ComputationalResultAnnotation" diff --git a/common/data_refinery_common/migrations/0030_update_sample_manufacturer.py b/common/data_refinery_common/migrations/0030_update_sample_manufacturer.py index a2d565c1b..a722fd145 100644 --- a/common/data_refinery_common/migrations/0030_update_sample_manufacturer.py +++ b/common/data_refinery_common/migrations/0030_update_sample_manufacturer.py @@ -4,7 +4,7 @@ def fix_typo_in_sample_manufacturer(apps, schema_editor): - """ Fixes affymetrix samples that have their manufacturer set to "AFFYMETRTIX" or "NEXTSEQ" """ + """Fixes affymetrix samples that have their manufacturer set to "AFFYMETRTIX" or "NEXTSEQ" """ Sample = apps.get_model("data_refinery_common", "Sample") Sample.objects.all().filter(manufacturer="AFFYMETRTIX").update(manufacturer="AFFYMETRIX") Sample.objects.all().filter(manufacturer="NEXTSEQ").update(manufacturer="ILLUMINA") diff --git a/common/data_refinery_common/migrations/0032_surveyedaccession.py b/common/data_refinery_common/migrations/0032_surveyedaccession.py index 01433e3cf..4c0534b83 100644 --- a/common/data_refinery_common/migrations/0032_surveyedaccession.py +++ b/common/data_refinery_common/migrations/0032_surveyedaccession.py @@ -26,6 +26,8 @@ class Migration(migrations.Migration): models.DateTimeField(default=django.utils.timezone.now, editable=False), ), ], - options={"db_table": "surveyed_accessions",}, + options={ + "db_table": "surveyed_accessions", + }, ), ] diff --git a/common/data_refinery_common/migrations/0034_cdfcorrectedaccession.py b/common/data_refinery_common/migrations/0034_cdfcorrectedaccession.py index b82cc3b17..fafbfcfa7 100644 --- a/common/data_refinery_common/migrations/0034_cdfcorrectedaccession.py +++ b/common/data_refinery_common/migrations/0034_cdfcorrectedaccession.py @@ -26,6 +26,8 @@ class Migration(migrations.Migration): models.DateTimeField(default=django.utils.timezone.now, editable=False), ), ], - options={"db_table": "cdf_corrected_accessions",}, + options={ + "db_table": "cdf_corrected_accessions", + }, ), ] diff --git a/common/data_refinery_common/migrations/0038_paginate_default_svd_algorithm.py b/common/data_refinery_common/migrations/0038_paginate_default_svd_algorithm.py index 32f711509..12d8cc22a 100644 --- a/common/data_refinery_common/migrations/0038_paginate_default_svd_algorithm.py +++ b/common/data_refinery_common/migrations/0038_paginate_default_svd_algorithm.py @@ -2,7 +2,7 @@ def batch_update(queryset, batch=1000, **changes): - """ Update per batch """ + """Update per batch""" is_done = False current = 0 @@ -18,21 +18,21 @@ def batch_update(queryset, batch=1000, **changes): def set_computed_file_svd_algorithm_arpack(apps, schema_editor): - """ Set svd_algorithm for computed files - ARPACK when is_compendia""" + """Set svd_algorithm for computed files - ARPACK when is_compendia""" ComputedFile = apps.get_model("data_refinery_common", "ComputedFile") arpack_queryset = ComputedFile.objects.filter(is_compendia=True).order_by("id") batch_update(arpack_queryset, svd_algorithm="ARPACK") def set_computed_file_svd_algorithm_none(apps, schema_editor): - """ Set svd_algorithm for computed files - None when not is_compendia""" + """Set svd_algorithm for computed files - None when not is_compendia""" ComputedFile = apps.get_model("data_refinery_common", "ComputedFile") none_queryset = ComputedFile.objects.filter(is_compendia=False).order_by("id") batch_update(none_queryset, svd_algorithm="NONE") def set_dataset_svd_algorithm_none(apps, schema_editor): - """ Apply default svd_algorithm via pagination to dataset """ + """Apply default svd_algorithm via pagination to dataset""" Dataset = apps.get_model("data_refinery_common", "Dataset") queryset = Dataset.objects.all().order_by("id") batch_update(queryset, svd_algorithm="NONE") diff --git a/common/data_refinery_common/migrations/0039_auto_20190925_1338.py b/common/data_refinery_common/migrations/0039_auto_20190925_1338.py index 0cc8875cc..5cf958235 100644 --- a/common/data_refinery_common/migrations/0039_auto_20190925_1338.py +++ b/common/data_refinery_common/migrations/0039_auto_20190925_1338.py @@ -10,5 +10,8 @@ class Migration(migrations.Migration): ] operations = [ - migrations.RemoveIndex(model_name="computedfile", name="computed_fi_filenam_64958d_idx",), + migrations.RemoveIndex( + model_name="computedfile", + name="computed_fi_filenam_64958d_idx", + ), ] diff --git a/common/data_refinery_common/migrations/0042_remove_experiment_organism_names.py b/common/data_refinery_common/migrations/0042_remove_experiment_organism_names.py index b6baaa467..3bc63cd19 100644 --- a/common/data_refinery_common/migrations/0042_remove_experiment_organism_names.py +++ b/common/data_refinery_common/migrations/0042_remove_experiment_organism_names.py @@ -10,5 +10,8 @@ class Migration(migrations.Migration): ] operations = [ - migrations.RemoveField(model_name="experiment", name="organism_names",), + migrations.RemoveField( + model_name="experiment", + name="organism_names", + ), ] diff --git a/common/data_refinery_common/migrations/0043_auto_20191108_2144.py b/common/data_refinery_common/migrations/0043_auto_20191108_2144.py index a7bd8afe2..50fe0e998 100644 --- a/common/data_refinery_common/migrations/0043_auto_20191108_2144.py +++ b/common/data_refinery_common/migrations/0043_auto_20191108_2144.py @@ -38,7 +38,10 @@ class Migration(migrations.Migration): ), ("is_public", models.BooleanField(default=True)), ], - options={"db_table": "compendium_results", "base_manager_name": "public_objects",}, + options={ + "db_table": "compendium_results", + "base_manager_name": "public_objects", + }, managers=[ ("objects", django.db.models.manager.Manager()), ("public_objects", django.db.models.manager.Manager()), @@ -68,7 +71,9 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "compendium_result_organism_associations",}, + options={ + "db_table": "compendium_result_organism_associations", + }, ), migrations.AddField( model_name="compendiumresult", diff --git a/common/data_refinery_common/migrations/0044_compendium_results.py b/common/data_refinery_common/migrations/0044_compendium_results.py index a2d2f57c0..886b6f6e9 100644 --- a/common/data_refinery_common/migrations/0044_compendium_results.py +++ b/common/data_refinery_common/migrations/0044_compendium_results.py @@ -2,7 +2,7 @@ def populate_compendium_results(apps, schema_editor): - """ Fetch existing compendia and create compendium results""" + """Fetch existing compendia and create compendium results""" CompendiumResult = apps.get_model("data_refinery_common", "CompendiumResult") CompendiumResultOrganismAssociation = apps.get_model( "data_refinery_common", "CompendiumResultOrganismAssociation" diff --git a/common/data_refinery_common/migrations/0045_svd_algorithm.py b/common/data_refinery_common/migrations/0045_svd_algorithm.py index 17511a7e4..30121683b 100644 --- a/common/data_refinery_common/migrations/0045_svd_algorithm.py +++ b/common/data_refinery_common/migrations/0045_svd_algorithm.py @@ -2,7 +2,7 @@ def svd_algorithm_fix(apps, schema_editor): - """ Fetch existing compendia and create compendium results""" + """Fetch existing compendia and create compendium results""" ComputedFile = apps.get_model("data_refinery_common", "ComputedFile") compendium_computed_files = ComputedFile.objects.filter( diff --git a/common/data_refinery_common/migrations/0046_compendium_version_number.py b/common/data_refinery_common/migrations/0046_compendium_version_number.py index a518ab21d..32ea9c903 100644 --- a/common/data_refinery_common/migrations/0046_compendium_version_number.py +++ b/common/data_refinery_common/migrations/0046_compendium_version_number.py @@ -4,7 +4,7 @@ def compendium_version_number_fix(apps, schema_editor): - """ Fetch existing compendia and fix their version_number.""" + """Fetch existing compendia and fix their version_number.""" CompendiumResult = apps.get_model("data_refinery_common", "CompendiumResult") # normalized compendia diff --git a/common/data_refinery_common/migrations/0047_clean_compendia_files.py b/common/data_refinery_common/migrations/0047_clean_compendia_files.py index 67e86e717..6fe20d338 100644 --- a/common/data_refinery_common/migrations/0047_clean_compendia_files.py +++ b/common/data_refinery_common/migrations/0047_clean_compendia_files.py @@ -3,7 +3,7 @@ def clean_compendium_files(apps, schema_editor): - """ We used to have more than one computed file for a compendium + """We used to have more than one computed file for a compendium Now we just want to keep the zipped file that lives on s3.""" CompendiumResult = apps.get_model("data_refinery_common", "CompendiumResult") ComputedFile = apps.get_model("data_refinery_common", "ComputedFile") diff --git a/common/data_refinery_common/migrations/0048_processorjob_abort.py b/common/data_refinery_common/migrations/0048_processorjob_abort.py index ce2e23f7d..240737294 100644 --- a/common/data_refinery_common/migrations/0048_processorjob_abort.py +++ b/common/data_refinery_common/migrations/0048_processorjob_abort.py @@ -2,7 +2,7 @@ def batch_update(queryset, batch=1000, **changes): - """ Update per batch """ + """Update per batch""" is_done = False current = 0 @@ -36,7 +36,9 @@ class Migration(migrations.Migration): operations = [ # 1. add fields with None as default value migrations.AddField( - model_name="processorjob", name="abort", field=models.BooleanField(null=True), + model_name="processorjob", + name="abort", + field=models.BooleanField(null=True), ), # 2. set default value in batches migrations.RunPython(set_default_abort_value), diff --git a/common/data_refinery_common/migrations/0050_remove_organismindex_s3_url.py b/common/data_refinery_common/migrations/0050_remove_organismindex_s3_url.py index d3e0d8dca..87d10a903 100644 --- a/common/data_refinery_common/migrations/0050_remove_organismindex_s3_url.py +++ b/common/data_refinery_common/migrations/0050_remove_organismindex_s3_url.py @@ -10,5 +10,8 @@ class Migration(migrations.Migration): ] operations = [ - migrations.RemoveField(model_name="organismindex", name="s3_url",), + migrations.RemoveField( + model_name="organismindex", + name="s3_url", + ), ] diff --git a/common/data_refinery_common/migrations/0051_remove_dataset_email_sent.py b/common/data_refinery_common/migrations/0051_remove_dataset_email_sent.py index 23bc26e5b..2f3fbcc52 100644 --- a/common/data_refinery_common/migrations/0051_remove_dataset_email_sent.py +++ b/common/data_refinery_common/migrations/0051_remove_dataset_email_sent.py @@ -10,5 +10,8 @@ class Migration(migrations.Migration): ] operations = [ - migrations.RemoveField(model_name="dataset", name="email_sent",), + migrations.RemoveField( + model_name="dataset", + name="email_sent", + ), ] diff --git a/common/data_refinery_common/migrations/0052_remove_invalid_tximport_associations.py b/common/data_refinery_common/migrations/0052_remove_invalid_tximport_associations.py index d2dea32e6..49de916bf 100644 --- a/common/data_refinery_common/migrations/0052_remove_invalid_tximport_associations.py +++ b/common/data_refinery_common/migrations/0052_remove_invalid_tximport_associations.py @@ -2,9 +2,9 @@ def remove_tximport_invalid_associations(apps, schema_editor): - """ We were associating tximport with all samples in an experiment + """We were associating tximport with all samples in an experiment even though some of them were unprocessed. - Ref https://github.com/AlexsLemonade/refinebio/issues/2054 """ + Ref https://github.com/AlexsLemonade/refinebio/issues/2054""" SampleResultAssociation = apps.get_model("data_refinery_common", "SampleResultAssociation") SampleResultAssociation.objects.filter( diff --git a/common/data_refinery_common/migrations/0054_datasetannotation.py b/common/data_refinery_common/migrations/0054_datasetannotation.py index 94820439d..139ba15ff 100644 --- a/common/data_refinery_common/migrations/0054_datasetannotation.py +++ b/common/data_refinery_common/migrations/0054_datasetannotation.py @@ -38,7 +38,10 @@ class Migration(migrations.Migration): ), ), ], - options={"db_table": "dataset_annotations", "base_manager_name": "public_objects",}, + options={ + "db_table": "dataset_annotations", + "base_manager_name": "public_objects", + }, managers=[ ("objects", django.db.models.manager.Manager()), ("public_objects", django.db.models.manager.Manager()), diff --git a/common/data_refinery_common/migrations/0056_auto_20200529_1230.py b/common/data_refinery_common/migrations/0056_auto_20200529_1230.py index 95b7ba293..7eae34282 100644 --- a/common/data_refinery_common/migrations/0056_auto_20200529_1230.py +++ b/common/data_refinery_common/migrations/0056_auto_20200529_1230.py @@ -11,9 +11,13 @@ class Migration(migrations.Migration): operations = [ migrations.AlterModelOptions( - name="datasetannotation", options={"base_manager_name": "objects"}, + name="datasetannotation", + options={"base_manager_name": "objects"}, + ), + migrations.AlterModelManagers( + name="datasetannotation", + managers=[], ), - migrations.AlterModelManagers(name="datasetannotation", managers=[],), migrations.AlterField( model_name="datasetannotation", name="is_public", diff --git a/common/data_refinery_common/migrations/0059_auto_20200819_1632.py b/common/data_refinery_common/migrations/0059_auto_20200819_1632.py index e1fa29bc4..286072fcd 100644 --- a/common/data_refinery_common/migrations/0059_auto_20200819_1632.py +++ b/common/data_refinery_common/migrations/0059_auto_20200819_1632.py @@ -42,7 +42,9 @@ class Migration(migrations.Migration): ("ontology_term", models.TextField(unique=True)), ("human_readable_name", models.TextField()), ], - options={"db_table": "ontology_terms",}, + options={ + "db_table": "ontology_terms", + }, ), migrations.AddField( model_name="experiment", @@ -150,7 +152,9 @@ class Migration(migrations.Migration): ), ), ], - options={"abstract": False,}, + options={ + "abstract": False, + }, ), migrations.CreateModel( name="ExperimentAttribute", @@ -207,6 +211,8 @@ class Migration(migrations.Migration): ), ), ], - options={"abstract": False,}, + options={ + "abstract": False, + }, ), ] diff --git a/common/data_refinery_common/migrations/0060_auto_20200828_1650.py b/common/data_refinery_common/migrations/0060_auto_20200828_1650.py index 257101985..608029107 100644 --- a/common/data_refinery_common/migrations/0060_auto_20200828_1650.py +++ b/common/data_refinery_common/migrations/0060_auto_20200828_1650.py @@ -11,7 +11,9 @@ class Migration(migrations.Migration): operations = [ migrations.RenameField( - model_name="organismindex", old_name="source_version", new_name="release_version", + model_name="organismindex", + old_name="source_version", + new_name="release_version", ), migrations.AddField( model_name="organismindex", diff --git a/common/data_refinery_common/migrations/0061_transcriptome_database_name.py b/common/data_refinery_common/migrations/0061_transcriptome_database_name.py index fd1183262..243df922e 100644 --- a/common/data_refinery_common/migrations/0061_transcriptome_database_name.py +++ b/common/data_refinery_common/migrations/0061_transcriptome_database_name.py @@ -27,7 +27,7 @@ def find_division_by_species(species: str, division_jsons: dict) -> str: def add_transcriptome_database_names(apps, schema_editor): - """ Updates `database_name` values for transcriptome indices """ + """Updates `database_name` values for transcriptome indices""" OrganismIndex = apps.get_model("data_refinery_common", "OrganismIndex") transcriptome_indices = OrganismIndex.objects.all() diff --git a/common/data_refinery_common/migrations/0064_auto_20210225_1622.py b/common/data_refinery_common/migrations/0064_auto_20210225_1622.py index a31efda44..33b169ff7 100644 --- a/common/data_refinery_common/migrations/0064_auto_20210225_1622.py +++ b/common/data_refinery_common/migrations/0064_auto_20210225_1622.py @@ -10,5 +10,8 @@ class Migration(migrations.Migration): ] operations = [ - migrations.AlterModelOptions(name="dataset", options={"base_manager_name": "objects"},), + migrations.AlterModelOptions( + name="dataset", + options={"base_manager_name": "objects"}, + ), ] diff --git a/common/data_refinery_common/migrations/0065_auto_20210324_1948.py b/common/data_refinery_common/migrations/0065_auto_20210324_1948.py index 170eed9cf..a1e8bac8f 100644 --- a/common/data_refinery_common/migrations/0065_auto_20210324_1948.py +++ b/common/data_refinery_common/migrations/0065_auto_20210324_1948.py @@ -24,13 +24,19 @@ class Migration(migrations.Migration): ), ), migrations.AlterField( - model_name="dataset", name="success", field=models.BooleanField(null=True), + model_name="dataset", + name="success", + field=models.BooleanField(null=True), ), migrations.AlterField( - model_name="datasetannotation", name="data", field=models.JSONField(default=dict), + model_name="datasetannotation", + name="data", + field=models.JSONField(default=dict), ), migrations.AlterField( - model_name="downloaderjob", name="success", field=models.BooleanField(null=True), + model_name="downloaderjob", + name="success", + field=models.BooleanField(null=True), ), migrations.AlterField( model_name="experiment", @@ -38,21 +44,33 @@ class Migration(migrations.Migration): field=models.JSONField(default=dict), ), migrations.AlterField( - model_name="experimentannotation", name="data", field=models.JSONField(default=dict), + model_name="experimentannotation", + name="data", + field=models.JSONField(default=dict), ), migrations.AlterField( - model_name="processor", name="environment", field=models.JSONField(default=dict), + model_name="processor", + name="environment", + field=models.JSONField(default=dict), ), migrations.AlterField( - model_name="processorjob", name="success", field=models.BooleanField(null=True), + model_name="processorjob", + name="success", + field=models.BooleanField(null=True), ), migrations.AlterField( - model_name="sample", name="protocol_info", field=models.JSONField(default=dict), + model_name="sample", + name="protocol_info", + field=models.JSONField(default=dict), ), migrations.AlterField( - model_name="sampleannotation", name="data", field=models.JSONField(default=dict), + model_name="sampleannotation", + name="data", + field=models.JSONField(default=dict), ), migrations.AlterField( - model_name="surveyjob", name="success", field=models.BooleanField(null=True), + model_name="surveyjob", + name="success", + field=models.BooleanField(null=True), ), ] diff --git a/common/data_refinery_common/migrations/0066_auto_20210611_1900.py b/common/data_refinery_common/migrations/0066_auto_20210611_1900.py index d035d93a9..ddaa01b5f 100644 --- a/common/data_refinery_common/migrations/0066_auto_20210611_1900.py +++ b/common/data_refinery_common/migrations/0066_auto_20210611_1900.py @@ -12,13 +12,19 @@ class Migration(migrations.Migration): operations = [ migrations.RenameField( - model_name="downloaderjob", old_name="nomad_job_id", new_name="batch_job_id", + model_name="downloaderjob", + old_name="nomad_job_id", + new_name="batch_job_id", ), migrations.RenameField( - model_name="processorjob", old_name="nomad_job_id", new_name="batch_job_id", + model_name="processorjob", + old_name="nomad_job_id", + new_name="batch_job_id", ), migrations.RenameField( - model_name="surveyjob", old_name="nomad_job_id", new_name="batch_job_id", + model_name="surveyjob", + old_name="nomad_job_id", + new_name="batch_job_id", ), migrations.AddField( model_name="downloaderjob", @@ -54,6 +60,8 @@ class Migration(migrations.Migration): ), ), migrations.AlterField( - model_name="surveyjob", name="ram_amount", field=models.IntegerField(default=1024), + model_name="surveyjob", + name="ram_amount", + field=models.IntegerField(default=1024), ), ] diff --git a/common/data_refinery_common/migrations/0067_dataset_notify_me.py b/common/data_refinery_common/migrations/0067_dataset_notify_me.py index f7894bfd8..78c2bf42f 100644 --- a/common/data_refinery_common/migrations/0067_dataset_notify_me.py +++ b/common/data_refinery_common/migrations/0067_dataset_notify_me.py @@ -11,6 +11,8 @@ class Migration(migrations.Migration): operations = [ migrations.AddField( - model_name="dataset", name="notify_me", field=models.BooleanField(default=True), + model_name="dataset", + name="notify_me", + field=models.BooleanField(default=True), ), ] diff --git a/common/data_refinery_common/migrations/0068_auto_20210729_1610.py b/common/data_refinery_common/migrations/0068_auto_20210729_1610.py index 463bdfed4..c4faa0ef8 100644 --- a/common/data_refinery_common/migrations/0068_auto_20210729_1610.py +++ b/common/data_refinery_common/migrations/0068_auto_20210729_1610.py @@ -21,6 +21,8 @@ class Migration(migrations.Migration): field=models.BigIntegerField(blank=True, null=True), ), migrations.AddField( - model_name="originalfile", name="md5", field=models.CharField(max_length=32, null=True), + model_name="originalfile", + name="md5", + field=models.CharField(max_length=32, null=True), ), ] diff --git a/common/data_refinery_common/migrations/0069_rename_compendia_version_computedfile_compendium_version.py b/common/data_refinery_common/migrations/0069_rename_compendia_version_computedfile_compendium_version.py index d0ec65ee6..99a389ec3 100644 --- a/common/data_refinery_common/migrations/0069_rename_compendia_version_computedfile_compendium_version.py +++ b/common/data_refinery_common/migrations/0069_rename_compendia_version_computedfile_compendium_version.py @@ -11,6 +11,8 @@ class Migration(migrations.Migration): operations = [ migrations.RenameField( - model_name="computedfile", old_name="compendia_version", new_name="compendium_version", + model_name="computedfile", + old_name="compendia_version", + new_name="compendium_version", ), ] diff --git a/common/data_refinery_common/models/api_token.py b/common/data_refinery_common/models/api_token.py index 628462367..4a0d1183d 100644 --- a/common/data_refinery_common/models/api_token.py +++ b/common/data_refinery_common/models/api_token.py @@ -6,7 +6,7 @@ class APIToken(models.Model): - """ Required for starting a smash job """ + """Required for starting a smash job""" # ID id = models.UUIDField(primary_key=True, default=uuid.uuid4, editable=False) @@ -19,7 +19,7 @@ class APIToken(models.Model): last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time diff --git a/common/data_refinery_common/models/base_models.py b/common/data_refinery_common/models/base_models.py index cf9df7afc..d5b7fb9a5 100644 --- a/common/data_refinery_common/models/base_models.py +++ b/common/data_refinery_common/models/base_models.py @@ -9,7 +9,7 @@ class TimeTrackedModel(models.Model): updated_at = models.DateTimeField() def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time diff --git a/common/data_refinery_common/models/command_progress.py b/common/data_refinery_common/models/command_progress.py index e103e4708..ed46032cd 100644 --- a/common/data_refinery_common/models/command_progress.py +++ b/common/data_refinery_common/models/command_progress.py @@ -8,7 +8,7 @@ class SurveyedAccession(models.Model): created_at = models.DateTimeField(editable=False, default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time @@ -26,7 +26,7 @@ class CdfCorrectedAccession(models.Model): created_at = models.DateTimeField(editable=False, default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time diff --git a/common/data_refinery_common/models/compendium_result.py b/common/data_refinery_common/models/compendium_result.py index 031136648..73e472b96 100644 --- a/common/data_refinery_common/models/compendium_result.py +++ b/common/data_refinery_common/models/compendium_result.py @@ -7,7 +7,7 @@ # Compendium Computational Result class CompendiumResult(models.Model): - """ Computational Result For A Compendium """ + """Computational Result For A Compendium""" class Meta: db_table = "compendium_results" @@ -61,7 +61,7 @@ def __str__(self): last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time @@ -70,5 +70,5 @@ def save(self, *args, **kwargs): # helper def get_computed_file(self): - """ Short hand method for getting the computed file for this compendium""" + """Short hand method for getting the computed file for this compendium""" return ComputedFile.objects.filter(result=self.result).first() diff --git a/common/data_refinery_common/models/computational_result.py b/common/data_refinery_common/models/computational_result.py index fcf0cbc9d..5a06b6b93 100644 --- a/common/data_refinery_common/models/computational_result.py +++ b/common/data_refinery_common/models/computational_result.py @@ -10,7 +10,7 @@ class ComputationalResult(models.Model): - """ Meta-information about the output of a computer process. (Ex Salmon) """ + """Meta-information about the output of a computer process. (Ex Salmon)""" class Meta: db_table = "computational_results" @@ -49,7 +49,7 @@ def __str__(self): last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time @@ -58,7 +58,7 @@ def save(self, *args, **kwargs): # Currently unused because of pre_delete signal for computed files def remove_computed_files_from_s3(self): - """ Removes all associated computed files from S3. + """Removes all associated computed files from S3. Use this before deleting a computational result. """ @@ -66,7 +66,7 @@ def remove_computed_files_from_s3(self): computed_file.delete_s3_file() def get_index_length(self): - """ Pull the index_length from one of the result annotations """ + """Pull the index_length from one of the result annotations""" annotations = ComputationalResultAnnotation.objects.filter(result=self) for annotation_json in annotations: @@ -78,7 +78,10 @@ def get_index_length(self): def get_quant_sf_file(self): return ( ComputedFile.objects.filter( - result=self, filename="quant.sf", s3_key__isnull=False, s3_bucket__isnull=False, + result=self, + filename="quant.sf", + s3_key__isnull=False, + s3_bucket__isnull=False, ) .order_by("-id") .first() diff --git a/common/data_refinery_common/models/computational_result_annotation.py b/common/data_refinery_common/models/computational_result_annotation.py index a30bfa9d2..44a29cd8f 100644 --- a/common/data_refinery_common/models/computational_result_annotation.py +++ b/common/data_refinery_common/models/computational_result_annotation.py @@ -5,7 +5,7 @@ class ComputationalResultAnnotation(models.Model): - """ Non-standard information associated with an ComputationalResult """ + """Non-standard information associated with an ComputationalResult""" class Meta: db_table = "computational_result_annotations" @@ -30,7 +30,7 @@ class Meta: last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time diff --git a/common/data_refinery_common/models/computed_file.py b/common/data_refinery_common/models/computed_file.py index f1ee79d62..6999c3625 100644 --- a/common/data_refinery_common/models/computed_file.py +++ b/common/data_refinery_common/models/computed_file.py @@ -20,7 +20,7 @@ class ComputedFile(models.Model): - """ A representation of a file created by a data-refinery process """ + """A representation of a file created by a data-refinery process""" class Meta: db_table = "computed_files" @@ -88,7 +88,7 @@ def __str__(self): last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time @@ -96,8 +96,7 @@ def save(self, *args, **kwargs): return super(ComputedFile, self).save(*args, **kwargs) def sync_to_s3(self, s3_bucket, s3_key, public=False) -> bool: - """ Syncs a file to AWS S3. - """ + """Syncs a file to AWS S3.""" if not settings.RUNNING_IN_CLOUD: return True @@ -108,7 +107,10 @@ def sync_to_s3(self, s3_bucket, s3_key, public=False) -> bool: extra_args["ACL"] = "public-read" S3.upload_file( - self.absolute_file_path, s3_bucket, s3_key, ExtraArgs=extra_args, + self.absolute_file_path, + s3_bucket, + s3_key, + ExtraArgs=extra_args, ) except Exception: logger.exception( @@ -126,7 +128,7 @@ def sync_to_s3(self, s3_bucket, s3_key, public=False) -> bool: return True def sync_from_s3(self, force=False, path=None): - """ Downloads a file from S3 to the local file system. + """Downloads a file from S3 to the local file system. Returns the absolute file path. """ path = path if path is not None else self.absolute_file_path @@ -219,19 +221,17 @@ def change_s3_location(self, new_bucket: str, new_key: str) -> bool: return True def calculate_sha1(self) -> None: - """ Calculate the SHA1 value of a given file. - """ + """Calculate the SHA1 value of a given file.""" self.sha1 = calculate_sha1(self.absolute_file_path) return self.sha1 def calculate_size(self) -> None: - """ Calculate the number of bytes in a given file. - """ + """Calculate the number of bytes in a given file.""" self.size_in_bytes = calculate_file_size(self.absolute_file_path) return self.size_in_bytes def delete_local_file(self, force=False): - """ Deletes a file from the path and actually removes it from the file system.""" + """Deletes a file from the path and actually removes it from the file system.""" if not settings.RUNNING_IN_CLOUD and not force: return @@ -271,8 +271,8 @@ def delete_s3_file(self, force=False): return True def get_synced_file_path(self, force=False, path=None): - """ Fetches the absolute file path to this ComputedFile, fetching from S3 if it - isn't already available locally. """ + """Fetches the absolute file path to this ComputedFile, fetching from S3 if it + isn't already available locally.""" if path: if os.path.exists(path): return path @@ -286,11 +286,11 @@ def get_synced_file_path(self, force=False, path=None): @property def s3_url(self): - """ Render the resulting HTTPS URL for the S3 object.""" + """Render the resulting HTTPS URL for the S3 object.""" return self.get_s3_url() def get_s3_url(self): - """ Render the resulting HTTPS URL for the S3 object.""" + """Render the resulting HTTPS URL for the S3 object.""" if (self.s3_key) and (self.s3_bucket): return "https://s3.amazonaws.com/" + self.s3_bucket + "/" + self.s3_key else: @@ -298,11 +298,11 @@ def get_s3_url(self): @property def download_url(self): - """ A temporary URL from which the file can be downloaded. """ + """A temporary URL from which the file can be downloaded.""" return self.create_download_url() def create_download_url(self): - """ Create a temporary URL from which the file can be downloaded.""" + """Create a temporary URL from which the file can be downloaded.""" if settings.RUNNING_IN_CLOUD and self.s3_bucket and self.s3_key: return S3.generate_presigned_url( ClientMethod="get_object", @@ -313,5 +313,5 @@ def create_download_url(self): return None def has_been_log2scaled(self): - """ Return true if this is a smashable file that has been log2 scaled """ + """Return true if this is a smashable file that has been log2 scaled""" return self.is_smashable and self.filename.endswith("lengthScaledTPM.tsv") diff --git a/common/data_refinery_common/models/contributions.py b/common/data_refinery_common/models/contributions.py index a9dc542aa..5a0a971d4 100644 --- a/common/data_refinery_common/models/contributions.py +++ b/common/data_refinery_common/models/contributions.py @@ -14,7 +14,7 @@ class Contribution(models.Model): created_at = models.DateTimeField(editable=False, default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" if not self.id: self.created_at = timezone.now() return super(Contribution, self).save(*args, **kwargs) diff --git a/common/data_refinery_common/models/dataset_annotation.py b/common/data_refinery_common/models/dataset_annotation.py index f282eca65..6aa59925e 100644 --- a/common/data_refinery_common/models/dataset_annotation.py +++ b/common/data_refinery_common/models/dataset_annotation.py @@ -5,8 +5,8 @@ class DatasetAnnotation(models.Model): - """ Semi-standard information associated with a Dataset. - IMPORTANT: This data shouldn't not be exposed through an API. """ + """Semi-standard information associated with a Dataset. + IMPORTANT: This data shouldn't not be exposed through an API.""" class Meta: db_table = "dataset_annotations" @@ -28,7 +28,7 @@ class Meta: last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.pk: self.created_at = current_time diff --git a/common/data_refinery_common/models/documents.py b/common/data_refinery_common/models/documents.py index 789886911..d767a66da 100644 --- a/common/data_refinery_common/models/documents.py +++ b/common/data_refinery_common/models/documents.py @@ -30,7 +30,11 @@ filter=["standard", "lowercase"], char_filter=["html_strip"], ) -standard_keyword = analyzer("standard_keyword", tokenizer="keyword", filter=[],) +standard_keyword = analyzer( + "standard_keyword", + tokenizer="keyword", + filter=[], +) @experiment_index.doc_type diff --git a/common/data_refinery_common/models/experiment_annotation.py b/common/data_refinery_common/models/experiment_annotation.py index dde785ecc..40d7d2e0c 100644 --- a/common/data_refinery_common/models/experiment_annotation.py +++ b/common/data_refinery_common/models/experiment_annotation.py @@ -5,7 +5,7 @@ class ExperimentAnnotation(models.Model): - """ Semi-standard information associated with an Experiment """ + """Semi-standard information associated with an Experiment""" class Meta: db_table = "experiment_annotations" @@ -28,7 +28,7 @@ class Meta: last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time diff --git a/common/data_refinery_common/models/jobs/downloader_job.py b/common/data_refinery_common/models/jobs/downloader_job.py index f06308180..c5d38ed5e 100644 --- a/common/data_refinery_common/models/jobs/downloader_job.py +++ b/common/data_refinery_common/models/jobs/downloader_job.py @@ -104,7 +104,7 @@ def get_samples(self) -> Set[Sample]: return samples def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time diff --git a/common/data_refinery_common/models/jobs/processor_job.py b/common/data_refinery_common/models/jobs/processor_job.py index abc1c1912..6c2ddde0b 100644 --- a/common/data_refinery_common/models/jobs/processor_job.py +++ b/common/data_refinery_common/models/jobs/processor_job.py @@ -88,7 +88,9 @@ class Meta: # If the job had data downloaded for it, this is the DownloaderJob that did so. downloader_job = models.ForeignKey( - "data_refinery_common.DownloaderJob", on_delete=models.SET_NULL, null=True, + "data_refinery_common.DownloaderJob", + on_delete=models.SET_NULL, + null=True, ) # If the job is retried, this is the id of the new job @@ -106,7 +108,7 @@ def get_samples(self) -> Set[Sample]: return samples def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time diff --git a/common/data_refinery_common/models/jobs/survey_job.py b/common/data_refinery_common/models/jobs/survey_job.py index 4dc65d7e0..fde501f80 100644 --- a/common/data_refinery_common/models/jobs/survey_job.py +++ b/common/data_refinery_common/models/jobs/survey_job.py @@ -59,7 +59,7 @@ class Meta: last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time @@ -67,11 +67,11 @@ def save(self, *args, **kwargs): return super(SurveyJob, self).save(*args, **kwargs) def get_properties(self) -> Dict: - """ Return all associated SurveyJobKeyValues as a dict""" + """Return all associated SurveyJobKeyValues as a dict""" return {pair.key: pair.value for pair in self.surveyjobkeyvalue_set.all()} def get_accession_code(self): - """ Return `experiment_accession_code`, the most important code.""" + """Return `experiment_accession_code`, the most important code.""" try: kvp = self.surveyjobkeyvalue_set.get(key="experiment_accession_code") return kvp.value diff --git a/common/data_refinery_common/models/organism.py b/common/data_refinery_common/models/organism.py index a119c3ddb..f1e2ec261 100644 --- a/common/data_refinery_common/models/organism.py +++ b/common/data_refinery_common/models/organism.py @@ -150,7 +150,7 @@ def __str__(self): return str(self.name) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time @@ -223,5 +223,5 @@ def get_object_for_name(cls, name: str, taxonomy_id=None): @classmethod def get_objects_with_qn_targets(cls): - """ Return a list of Organisms who already have valid QN targets associated with them. """ + """Return a list of Organisms who already have valid QN targets associated with them.""" return Organism.objects.all().filter(qn_target__isnull=False) diff --git a/common/data_refinery_common/models/organism_index.py b/common/data_refinery_common/models/organism_index.py index b53ef2204..475cc3e12 100644 --- a/common/data_refinery_common/models/organism_index.py +++ b/common/data_refinery_common/models/organism_index.py @@ -6,7 +6,7 @@ class OrganismIndex(models.Model): - """ A special type of process result, necessary for processing other SRA samples """ + """A special type of process result, necessary for processing other SRA samples""" class Meta: db_table = "organism_index" @@ -62,11 +62,11 @@ def __str__(self): last_modified = models.DateTimeField(default=timezone.now) def get_computed_file(self): - """ Short hand method for getting the computed file for this organism index""" + """Short hand method for getting the computed file for this organism index""" return self.result.computedfile_set.first() def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time diff --git a/common/data_refinery_common/models/original_file.py b/common/data_refinery_common/models/original_file.py index 5fb1e582f..eba916929 100644 --- a/common/data_refinery_common/models/original_file.py +++ b/common/data_refinery_common/models/original_file.py @@ -20,7 +20,7 @@ class OriginalFile(models.Model): - """ A representation of a file from an external source """ + """A representation of a file from an external source""" class Meta: db_table = "original_files" @@ -76,7 +76,7 @@ def __str__(self): last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time @@ -84,8 +84,8 @@ def save(self, *args, **kwargs): return super(OriginalFile, self).save(*args, **kwargs) def set_downloaded(self, absolute_file_path, filename=None): - """ Marks the file as downloaded, if `filename` is not provided it will - be parsed from the `absolute_file_path` """ + """Marks the file as downloaded, if `filename` is not provided it will + be parsed from the `absolute_file_path`""" self.is_downloaded = True self.is_archive = FileUtils.is_archive(absolute_file_path) self.absolute_file_path = absolute_file_path @@ -95,46 +95,42 @@ def set_downloaded(self, absolute_file_path, filename=None): self.save() def calculate_sha1(self) -> None: - """ Calculate the SHA1 value of a given file. - """ + """Calculate the SHA1 value of a given file.""" self.sha1 = calculate_sha1(self.absolute_file_path) return self.sha1 def calculate_md5(self) -> None: - """ Calculate the MD5 value of a given file. - """ + """Calculate the MD5 value of a given file.""" self.md5 = calculate_md5(self.absolute_file_path) return self.md5 def calculate_sha1_and_md5(self) -> None: - """ Calculate the MD5 and SHA1 value of a given file at the same time. - """ + """Calculate the MD5 and SHA1 value of a given file at the same time.""" self.sha1, self.md5 = calculate_sha1_and_md5(self.absolute_file_path) return self.sha1, self.md5 def calculate_size(self) -> None: - """ Calculate the number of bytes in a given file. - """ + """Calculate the number of bytes in a given file.""" self.size_in_bytes = calculate_file_size(self.absolute_file_path) return self.size_in_bytes def get_display_name(self): - """ For dev convenience """ + """For dev convenience""" if not self.filename: return self.source_filename else: return self.filename def get_extension(self): - """ Returns the lowercased extension of the filename - Thanks to https://stackoverflow.com/a/541408/763705 """ + """Returns the lowercased extension of the filename + Thanks to https://stackoverflow.com/a/541408/763705""" return FileUtils.get_extension(self.filename) def is_blacklisted(self): return self.get_extension() in [".xml", ".chp", ".exp"] def delete_local_file(self): - """ Deletes this file from the local file system.""" + """Deletes this file from the local file system.""" try: os.remove(self.absolute_file_path) except OSError: @@ -276,8 +272,7 @@ def needs_downloading(self, own_processor_id=None) -> bool: return self.needs_processing(own_processor_id) def is_affy_data(self) -> bool: - """Return true if original_file is a CEL file or a gzipped CEL file. - """ + """Return true if original_file is a CEL file or a gzipped CEL file.""" upper_name = self.source_filename.upper() return (len(upper_name) > 4 and upper_name[-4:] == ".CEL") or ( len(upper_name) > 7 and upper_name[-7:] == ".CEL.GZ" diff --git a/common/data_refinery_common/models/sample_annotation.py b/common/data_refinery_common/models/sample_annotation.py index 318797950..092f83a94 100644 --- a/common/data_refinery_common/models/sample_annotation.py +++ b/common/data_refinery_common/models/sample_annotation.py @@ -5,7 +5,7 @@ class SampleAnnotation(models.Model): - """ Semi-standard information associated with a Sample """ + """Semi-standard information associated with a Sample""" class Meta: db_table = "sample_annotations" @@ -28,7 +28,7 @@ class Meta: last_modified = models.DateTimeField(default=timezone.now) def save(self, *args, **kwargs): - """ On save, update timestamps """ + """On save, update timestamps""" current_time = timezone.now() if not self.id: self.created_at = current_time diff --git a/common/data_refinery_common/models/test_ontology_term.py b/common/data_refinery_common/models/test_ontology_term.py index 65340349d..4b2200059 100644 --- a/common/data_refinery_common/models/test_ontology_term.py +++ b/common/data_refinery_common/models/test_ontology_term.py @@ -108,12 +108,14 @@ def test_import_cellosaurus(self, mock_api_call): created_terms = OntologyTerm.import_entire_ontology("cvcl") self.assertEqual( - OntologyTerm.objects.all().count(), 34, # This is the number I counted in the file + OntologyTerm.objects.all().count(), + 34, # This is the number I counted in the file ) self.assertEqual(OntologyTerm.objects.all().count(), created_terms) self.assertEqual( - "#W7079", OntologyTerm.get_or_create_from_api("CVCL:E549").human_readable_name, + "#W7079", + OntologyTerm.get_or_create_from_api("CVCL:E549").human_readable_name, ) mock_api_call.assert_not_called() diff --git a/common/data_refinery_common/models/test_organisms.py b/common/data_refinery_common/models/test_organisms.py index b84dbaa65..4fc14540b 100644 --- a/common/data_refinery_common/models/test_organisms.py +++ b/common/data_refinery_common/models/test_organisms.py @@ -231,8 +231,7 @@ def test_invalid_ids_cause_exceptions(self, mock_get): @patch("data_refinery_common.models.organism.requests.get") def test_unfound_names_raise(self, mock_get): - """If we can't find the taxonomy id, it's likely a bad organism name. - """ + """If we can't find the taxonomy id, it's likely a bad organism name.""" mock_get.return_value = Mock(ok=True) mock_get.return_value.text = ESEARCH_NOT_FOUND_XML diff --git a/common/data_refinery_common/performant_pagination/runtests/settings.py b/common/data_refinery_common/performant_pagination/runtests/settings.py index 5d04dfc1e..85c892c6f 100644 --- a/common/data_refinery_common/performant_pagination/runtests/settings.py +++ b/common/data_refinery_common/performant_pagination/runtests/settings.py @@ -11,7 +11,12 @@ # https://dev.to/rubyflewtoo/upgrading-to-django-3-2-and-fixing-defaultautofield-warnings-518n DEFAULT_AUTO_FIELD = "django.db.models.AutoField" -DATABASES = {"default": {"ENGINE": "django.db.backends.sqlite3", "NAME": "sqlite.db",}} +DATABASES = { + "default": { + "ENGINE": "django.db.backends.sqlite3", + "NAME": "sqlite.db", + } +} SECRET_KEY = "p!_%jd*snxvam@gs2(+w*mzg+x231s37rz#v*=f&kz07nn81j5" diff --git a/common/data_refinery_common/settings.py b/common/data_refinery_common/settings.py index 8989db263..1b8107751 100644 --- a/common/data_refinery_common/settings.py +++ b/common/data_refinery_common/settings.py @@ -99,10 +99,18 @@ # https://docs.djangoproject.com/en/1.10/ref/settings/#auth-password-validators AUTH_PASSWORD_VALIDATORS = [ - {"NAME": ("django.contrib.auth.password_validation" + ".UserAttributeSimilarityValidator"),}, - {"NAME": ("django.contrib.auth.password_validation." + "MinimumLengthValidator"),}, - {"NAME": ("django.contrib.auth.password_validation" + ".CommonPasswordValidator"),}, - {"NAME": ("django.contrib.auth.password_validation" + ".NumericPasswordValidator"),}, + { + "NAME": ("django.contrib.auth.password_validation" + ".UserAttributeSimilarityValidator"), + }, + { + "NAME": ("django.contrib.auth.password_validation." + "MinimumLengthValidator"), + }, + { + "NAME": ("django.contrib.auth.password_validation" + ".CommonPasswordValidator"), + }, + { + "NAME": ("django.contrib.auth.password_validation" + ".NumericPasswordValidator"), + }, ] diff --git a/common/data_refinery_common/signals.py b/common/data_refinery_common/signals.py index a8cc5809e..bb1c90d62 100644 --- a/common/data_refinery_common/signals.py +++ b/common/data_refinery_common/signals.py @@ -9,6 +9,5 @@ @receiver(pre_delete, sender=ComputedFile) def remove_file_from_s3(sender, instance, **kwargs): - """ When the local model is about to be deleted, try to delete the s3 file - """ + """When the local model is about to be deleted, try to delete the s3 file""" instance.delete_s3_file() diff --git a/common/data_refinery_common/test_utils.py b/common/data_refinery_common/test_utils.py index 71c8772ee..bcf9ee01a 100644 --- a/common/data_refinery_common/test_utils.py +++ b/common/data_refinery_common/test_utils.py @@ -89,7 +89,7 @@ def test_readable_affymetrix_names(self): self.assertTrue(readable_platform_names["xenopuslaevis"] == expected_readable_name) def test_get_normalized_platform(self): - """ Test a particular normaization we need to perform """ + """Test a particular normaization we need to perform""" self.assertEqual(utils.get_normalized_platform("hugene10stv1"), "hugene10st") self.assertEqual(utils.get_normalized_platform("hugene10stv2"), "hugene10st") diff --git a/common/data_refinery_common/utils.py b/common/data_refinery_common/utils.py index b573d3cdb..318ad6ae9 100644 --- a/common/data_refinery_common/utils.py +++ b/common/data_refinery_common/utils.py @@ -28,7 +28,7 @@ def get_env_variable(var_name: str, default: str = None) -> str: - """ Get an environment variable or return a default value """ + """Get an environment variable or return a default value""" try: return os.environ[var_name] except KeyError: @@ -167,7 +167,9 @@ def get_readable_affymetrix_names( READABLE_PLATFORM_NAMES = {} with open(mapping_csv, encoding="utf-8") as mapping_file: - reader = csv.reader(mapping_file,) + reader = csv.reader( + mapping_file, + ) for line in reader: # Skip the header row # Lines are 1 indexed, #BecauseCSV @@ -194,7 +196,9 @@ def get_affymetrix_annotation_package_name_overrides( ANNOTATION_PACKAGE_OVERRIDES = {} with open(overrides_csv, encoding="utf-8") as overrides_file: - reader = csv.reader(overrides_file,) + reader = csv.reader( + overrides_file, + ) for line in reader: # Skip the header row # Lines are 1 indexed, #BecauseCSV @@ -344,11 +348,13 @@ def get_https_sra_download(run_accession: str): def load_blacklist(blacklist_csv: str = "config/RNASeqRunBlackList.csv"): - """ Loads the SRA run blacklist """ + """Loads the SRA run blacklist""" blacklisted_samples = [] with open(blacklist_csv, encoding="utf-8") as blacklist_file: - reader = csv.reader(blacklist_file,) + reader = csv.reader( + blacklist_file, + ) for line in reader: # Skip the header row # Lines are 1 indexed, #BecauseCSV @@ -361,7 +367,7 @@ def load_blacklist(blacklist_csv: str = "config/RNASeqRunBlackList.csv"): def queryset_page_iterator(queryset, page_size=2000): - """ use the performant paginator to iterate over each page in a queryset """ + """use the performant paginator to iterate over each page in a queryset""" paginator = PerformantPaginator(queryset, page_size) page = paginator.page() while True: @@ -374,7 +380,7 @@ def queryset_page_iterator(queryset, page_size=2000): def queryset_iterator(queryset, page_size=2000): - """ use the performant paginator to iterate over a queryset """ + """use the performant paginator to iterate over a queryset""" for page in queryset_page_iterator(queryset, page_size): for item in page: yield item @@ -407,7 +413,7 @@ def download_file( if retry < max_retries: # After the retry-th failed attempt, retry downloading after # k*backoff_factor, where k is a random integer between 0 and 2^retry − 1. - k = random.randint(0, 2 ** retry - 1) + k = random.randint(0, 2**retry - 1) sleep_timeout = min(k * backoff_factor, max_sleep_timeout) time.sleep(sleep_timeout) diff --git a/foreman/data_refinery_foreman/foreman/job_control.py b/foreman/data_refinery_foreman/foreman/job_control.py index e4a46de59..1c0697fe9 100644 --- a/foreman/data_refinery_foreman/foreman/job_control.py +++ b/foreman/data_refinery_foreman/foreman/job_control.py @@ -59,7 +59,7 @@ def send_janitor_jobs(): def clean_database(): - """ Removes duplicated objects that may have appeared through race, OOM, bugs, etc. + """Removes duplicated objects that may have appeared through race, OOM, bugs, etc. See: https://github.com/AlexsLemonade/refinebio/issues/1183 """ diff --git a/foreman/data_refinery_foreman/foreman/job_requeuing.py b/foreman/data_refinery_foreman/foreman/job_requeuing.py index 40e9ea7de..e7df92f0a 100644 --- a/foreman/data_refinery_foreman/foreman/job_requeuing.py +++ b/foreman/data_refinery_foreman/foreman/job_requeuing.py @@ -245,7 +245,9 @@ def requeue_survey_job(last_job: SurveyJob) -> None: for keyvalue in keyvalues: SurveyJobKeyValue.objects.get_or_create( - survey_job=new_job, key=keyvalue.key, value=keyvalue.value, + survey_job=new_job, + key=keyvalue.key, + value=keyvalue.value, ) logger.debug( diff --git a/foreman/data_refinery_foreman/foreman/management/commands/check_computed_files.py b/foreman/data_refinery_foreman/foreman/management/commands/check_computed_files.py index 18cf65aac..9019571ba 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/check_computed_files.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/check_computed_files.py @@ -39,7 +39,7 @@ def check_item(computed_file): class Command(BaseCommand): def handle(self, *args, **options): - """ We found some computed files that seem to be in S3 but they don't exist for some reason. + """We found some computed files that seem to be in S3 but they don't exist for some reason. This command will check all of them and ensure they exists in S3. For those that don't exist it will clear their `s3_key`/`s3_bucket` fields. ref https://github.com/AlexsLemonade/refinebio/issues/1696 diff --git a/foreman/data_refinery_foreman/foreman/management/commands/correct_affy_cdfs.py b/foreman/data_refinery_foreman/foreman/management/commands/correct_affy_cdfs.py index 3f6063538..a719247ae 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/correct_affy_cdfs.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/correct_affy_cdfs.py @@ -42,8 +42,7 @@ def add_arguments(self, parser): ) def handle(self, *args, **options): - """Re-surveys GEO experiments containing samples with incorrect platform information. - """ + """Re-surveys GEO experiments containing samples with incorrect platform information.""" # Check against CDF corrected accessions table to prevent recorrection of the same samples. corrected_experiments = CdfCorrectedAccession.objects.all().values("accession_code") diff --git a/foreman/data_refinery_foreman/foreman/management/commands/create_compendia.py b/foreman/data_refinery_foreman/foreman/management/commands/create_compendia.py index 613c7d36b..258d596a0 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/create_compendia.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/create_compendia.py @@ -83,8 +83,8 @@ def get_target_organisms(organisms): def group_organisms_by_biggest_platform(target_organisms): - """ Create groups of organisms that share the same platform - ref https://github.com/AlexsLemonade/refinebio/issues/1736 """ + """Create groups of organisms that share the same platform + ref https://github.com/AlexsLemonade/refinebio/issues/1736""" result = [] # process the organisms with the most platforms first @@ -122,8 +122,8 @@ def group_organisms_by_biggest_platform(target_organisms): def get_organism_microarray_platforms(organism): - """ Returns the accession codes of the Affymetrix microarray platforms associated with an - organism. Ordered by the number of samples for each platform in descending order """ + """Returns the accession codes of the Affymetrix microarray platforms associated with an + organism. Ordered by the number of samples for each platform in descending order""" return ( organism.sample_set.filter(has_raw=True, technology="MICROARRAY", is_processed=True) .values("platform_accession_code") @@ -165,7 +165,7 @@ def create_job_for_organism(organisms: List[Organism], svd_algorithm="ARPACK"): def get_dataset(organisms: List[Organism]): - """ Builds a dataset with the samples associated with the given organisms """ + """Builds a dataset with the samples associated with the given organisms""" dataset = {} filter_query = Q() diff --git a/foreman/data_refinery_foreman/foreman/management/commands/create_missing_downloader_jobs.py b/foreman/data_refinery_foreman/foreman/management/commands/create_missing_downloader_jobs.py index 174c42da3..5f0851d85 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/create_missing_downloader_jobs.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/create_missing_downloader_jobs.py @@ -32,7 +32,7 @@ def add_arguments(self, parser): ) def handle(self, *args, **options): - """ Requeues downloader jobs for samples that haven't been processed and their original files + """Requeues downloader jobs for samples that haven't been processed and their original files have no no downloader jobs associated with them """ supported_microarray_platforms = [ diff --git a/foreman/data_refinery_foreman/foreman/management/commands/create_quantpendia.py b/foreman/data_refinery_foreman/foreman/management/commands/create_quantpendia.py index d598ac3e9..3c0465c0a 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/create_quantpendia.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/create_quantpendia.py @@ -49,7 +49,10 @@ def create_job_for_organism(organism: Organism): def build_dataset(organism: Organism): data = {} - experiments = Experiment.objects.filter(organisms=organism, technology="RNA-SEQ",).distinct() + experiments = Experiment.objects.filter( + organisms=organism, + technology="RNA-SEQ", + ).distinct() for experiment_page in queryset_page_iterator(experiments): for experiment in experiment_page: diff --git a/foreman/data_refinery_foreman/foreman/management/commands/fix_compendia.py b/foreman/data_refinery_foreman/foreman/management/commands/fix_compendia.py index ee8b06b69..ba42d87ad 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/fix_compendia.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/fix_compendia.py @@ -31,7 +31,10 @@ def transfer_computed_file(computed_file): copy_source = {"Bucket": old_s3_bucket, "Key": s3_key} # keep the same key, just different S3.copy( - copy_source, S3_COMPENDIA_BUCKET_NAME, s3_key, ExtraArgs={"StorageClass": "STANDARD_IA"}, + copy_source, + S3_COMPENDIA_BUCKET_NAME, + s3_key, + ExtraArgs={"StorageClass": "STANDARD_IA"}, ) # 2. update computed file diff --git a/foreman/data_refinery_foreman/foreman/management/commands/generate_dataset_report.py b/foreman/data_refinery_foreman/foreman/management/commands/generate_dataset_report.py index 818bb7ad9..b14910e2a 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/generate_dataset_report.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/generate_dataset_report.py @@ -10,7 +10,9 @@ qs = ( Dataset.processed_filtered_objects.filter(created_at__gt=START_DATE) - .annotate(week=ExtractWeek("created_at"),) + .annotate( + week=ExtractWeek("created_at"), + ) .values("week") .annotate(n=Count("pk")) .order_by("week") diff --git a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py index 434b12ceb..8b1d0c93a 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py @@ -56,7 +56,9 @@ def import_keywords(keywords: Dict, source: Contribution): for keyword in keywords: SampleKeyword.objects.get_or_create( - sample=sample, source=source, name=OntologyTerm.get_or_create_from_api(keyword), + sample=sample, + source=source, + name=OntologyTerm.get_or_create_from_api(keyword), ) for experiment in dirty_experiments: diff --git a/foreman/data_refinery_foreman/foreman/management/commands/import_ontology.py b/foreman/data_refinery_foreman/foreman/management/commands/import_ontology.py index 37ae04b20..b206a02d8 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/import_ontology.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/import_ontology.py @@ -17,7 +17,9 @@ class Command(BaseCommand): def add_arguments(self, parser): parser.add_argument( - "--ontology", type=str, help=("The prefix of the ontology to import (e.g. UO)."), + "--ontology", + type=str, + help=("The prefix of the ontology to import (e.g. UO)."), ) def handle(self, *args, **options): diff --git a/foreman/data_refinery_foreman/foreman/management/commands/mark_samples_unprocessable.py b/foreman/data_refinery_foreman/foreman/management/commands/mark_samples_unprocessable.py index be2c9015a..35e960251 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/mark_samples_unprocessable.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/mark_samples_unprocessable.py @@ -16,17 +16,17 @@ def mark_unprocessable_samples(): """This function performs the function explained at the head of this file. - It does so by following this general strategy: - 1. Find samples that have is_processed=True - 2. Find the most recent processor job for that sample. - 3. Check that processor job's failure reason and mark - the sample accordingly. - - Along the way it will also populate the following fields: - * last_processor_job - * last_downloader_job - * most_recent_smashable_file - * most_recent_quant_file + It does so by following this general strategy: + 1. Find samples that have is_processed=True + 2. Find the most recent processor job for that sample. + 3. Check that processor job's failure reason and mark + the sample accordingly. + + Along the way it will also populate the following fields: + * last_processor_job + * last_downloader_job + * most_recent_smashable_file + * most_recent_quant_file """ unprocessed_samples = Sample.objects.filter(is_processed=False).prefetch_related( "original_files__processor_jobs" diff --git a/foreman/data_refinery_foreman/foreman/management/commands/organism_shepherd.py b/foreman/data_refinery_foreman/foreman/management/commands/organism_shepherd.py index 1366078c7..22bf6a841 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/organism_shepherd.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/organism_shepherd.py @@ -222,8 +222,7 @@ def add_arguments(self, parser): ) def handle(self, *args, **options): - """Requeues all unprocessed RNA-Seq samples for an organism. - """ + """Requeues all unprocessed RNA-Seq samples for an organism.""" if options["organism_name"] is None: logger.error("You must specify an organism-name.") sys.exit(1) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/retry_samples.py b/foreman/data_refinery_foreman/foreman/management/commands/retry_samples.py index bce0a6221..4924c44ee 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/retry_samples.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/retry_samples.py @@ -66,7 +66,7 @@ def retry_by_accession_codes(accession_codes_param): def retry_by_regex(pattern, last_modified=None): - """ Finds the samples where the failure reason of the latest processor job that was applied to them + """Finds the samples where the failure reason of the latest processor job that was applied to them matches the given regex https://docs.djangoproject.com/en/dev/ref/models/querysets/#regex """ @@ -158,7 +158,7 @@ def add_arguments(self, parser): ) def handle(self, *args, **options): - """ Re-queues all unprocessed RNA-Seq samples for an organism. """ + """Re-queues all unprocessed RNA-Seq samples for an organism.""" if options["source_database"]: retry_by_source_database(options["source_database"]) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/run_tximport.py b/foreman/data_refinery_foreman/foreman/management/commands/run_tximport.py index 6b93b4324..aa0a2bd58 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/run_tximport.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/run_tximport.py @@ -65,8 +65,7 @@ def run_tximport_if_eligible(experiment: Experiment, dispatch_jobs=True) -> bool def run_tximport_for_all_eligible_experiments(dispatch_jobs=True): - """Creates a tximport job for all eligible experiments. - """ + """Creates a tximport job for all eligible experiments.""" eligible_experiments = ( Experiment.objects.annotate(num_organisms=Count("organisms")) .filter(num_organisms=1, technology="RNA-SEQ", num_processed_samples=0) @@ -101,8 +100,7 @@ def run_tximport_for_all_eligible_experiments(dispatch_jobs=True): def run_tximport_for_list(accession_codes: List[str], dispatch_jobs=True): - """Creates a tximport job for all experiments in the list of accession_codes that are eligble. - """ + """Creates a tximport job for all experiments in the list of accession_codes that are eligble.""" accession_codes = accession_codes.split(",") creation_count = 0 print(accession_codes) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/stop_jobs.py b/foreman/data_refinery_foreman/foreman/management/commands/stop_jobs.py index b147a8368..dea026781 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/stop_jobs.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/stop_jobs.py @@ -41,7 +41,9 @@ def stop_jobs(job_ids, job_type, reason): class Command(BaseCommand): def add_arguments(self, parser): parser.add_argument( - "job-ids", type=str, help=("Comma separated job IDs that need to be stopped."), + "job-ids", + type=str, + help=("Comma separated job IDs that need to be stopped."), ) parser.add_argument( diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_rerun_salmon_old_samples.py b/foreman/data_refinery_foreman/foreman/management/commands/test_rerun_salmon_old_samples.py index c964e25eb..4f1b3a2bc 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/test_rerun_salmon_old_samples.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/test_rerun_salmon_old_samples.py @@ -27,7 +27,7 @@ def setup_experiment(new_version_accessions: List[str], old_version_accessions: List[str]) -> Dict: - """ Create an experiment where some samples were processed with the newest version of salmon and + """Create an experiment where some samples were processed with the newest version of salmon and other with an older one. """ # Create the experiment diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_retry_samples.py b/foreman/data_refinery_foreman/foreman/management/commands/test_retry_samples.py index ade3112f7..ef5023a34 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/test_retry_samples.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/test_retry_samples.py @@ -27,7 +27,7 @@ def setup_experiment() -> Dict: - """ Create an experiment with two samples where one of them has a processor job that failed + """Create an experiment with two samples where one of them has a processor job that failed because Batch restarted it.""" # Create the experiment @@ -92,8 +92,7 @@ def setup_experiment() -> Dict: class RetrySamples(TestCase): - """ - """ + """ """ def test(self): setup_experiment() diff --git a/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py b/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py index b365e2c73..00d8a637d 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py @@ -30,8 +30,7 @@ def add_arguments(self, parser): ) def handle(self, *args, **options): - """Refreshes the metadata for all experiments, or experiments from a specific database - """ + """Refreshes the metadata for all experiments, or experiments from a specific database""" possible_source_databases = ["ARRAY_EXPRESS", "GEO", "SRA"] if options.get("source_database", None) is None: @@ -63,7 +62,9 @@ def handle(self, *args, **options): elif experiment.source_database == "GEO": gse = GEOparse.get_GEO( - experiment.accession_code, destdir="/tmp/management", silent=True, + experiment.accession_code, + destdir="/tmp/management", + silent=True, ) GeoSurveyor._apply_metadata_to_experiment(experiment, gse) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/update_sample_metadata.py b/foreman/data_refinery_foreman/foreman/management/commands/update_sample_metadata.py index a676829c6..db313e801 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/update_sample_metadata.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/update_sample_metadata.py @@ -30,8 +30,7 @@ def add_arguments(self, parser): ) def handle(self, *args, **options): - """Refreshes the metadata for all samples, or samples from a specific database - """ + """Refreshes the metadata for all samples, or samples from a specific database""" possible_source_databases = ["ARRAY_EXPRESS", "GEO", "SRA"] if options.get("source_database", None) is None: diff --git a/foreman/data_refinery_foreman/settings.py b/foreman/data_refinery_foreman/settings.py index 7a489facc..c6d903bf2 100644 --- a/foreman/data_refinery_foreman/settings.py +++ b/foreman/data_refinery_foreman/settings.py @@ -108,10 +108,18 @@ # https://docs.djangoproject.com/en/1.10/ref/settings/#auth-password-validators AUTH_PASSWORD_VALIDATORS = [ - {"NAME": "django.contrib.auth.password_validation.UserAttributeSimilarityValidator",}, - {"NAME": "django.contrib.auth.password_validation.MinimumLengthValidator",}, - {"NAME": "django.contrib.auth.password_validation.CommonPasswordValidator",}, - {"NAME": "django.contrib.auth.password_validation.NumericPasswordValidator",}, + { + "NAME": "django.contrib.auth.password_validation.UserAttributeSimilarityValidator", + }, + { + "NAME": "django.contrib.auth.password_validation.MinimumLengthValidator", + }, + { + "NAME": "django.contrib.auth.password_validation.CommonPasswordValidator", + }, + { + "NAME": "django.contrib.auth.password_validation.NumericPasswordValidator", + }, ] diff --git a/foreman/data_refinery_foreman/surveyor/external_source.py b/foreman/data_refinery_foreman/surveyor/external_source.py index 3d9e47a6d..eab426e26 100644 --- a/foreman/data_refinery_foreman/surveyor/external_source.py +++ b/foreman/data_refinery_foreman/surveyor/external_source.py @@ -106,8 +106,7 @@ def queue_downloader_job_for_original_files( experiment_accession_code: str = None, is_transcriptome: bool = False, ): - """Creates a single DownloaderJob with multiple files to download. - """ + """Creates a single DownloaderJob with multiple files to download.""" # Transcriptome is a special case because there's no sample_object. # It's alright to re-process transcriptome indices. if is_transcriptome: diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 5949f0cb9..433206de9 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -265,7 +265,7 @@ def extract_title(sample: Dict, priority_field: str = None) -> str: - """ Given a flat sample dictionary, find the title """ + """Given a flat sample dictionary, find the title""" if priority_field: title_fields = [priority_field] + [tf for tf in TITLE_FIELDS if tf != priority_field] else: @@ -288,7 +288,7 @@ def extract_title(sample: Dict, priority_field: str = None) -> str: def create_variants(fields_list: List): - """ Given a list of strings, create variations likely to give metadata hits. + """Given a list of strings, create variations likely to give metadata hits. Ex, given 'cell line', add the ability to hit on 'characteristic [cell_line]' as well. """ @@ -324,7 +324,7 @@ def create_variants(fields_list: List): def parse_sdrf(sdrf_url: str) -> List: - """ Given a URL to an SDRF file, download parses it into JSON. """ + """Given a URL to an SDRF file, download parses it into JSON.""" try: sdrf_response = requests_retry_session().get(sdrf_url, timeout=60) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index 11fbb0586..777a199ce 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -190,7 +190,7 @@ def gather_file_report(run_accession: str) -> List[Dict]: This endpoint returns a weird format, so some custom parsing is required: run_accession fastq_ftp fastq_bytes fastq_md5 submitted_ftp submitted_bytes submitted_md5 sra_ftp sra_bytes sra_md5 - SRR7353755 ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_2.fastq.gz 25176;2856704;3140575 7ef1ba010dcb679217112efa380798b2;6bc5651b7103306d4d65018180ab8d0d;3856c14164612d9879d576a046a9879f """ + SRR7353755 ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_2.fastq.gz 25176;2856704;3140575 7ef1ba010dcb679217112efa380798b2;6bc5651b7103306d4d65018180ab8d0d;3856c14164612d9879d576a046a9879f""" response = utils.requests_retry_session().get( ENA_FILE_REPORT_URL_TEMPLATE.format(accession=run_accession) ) diff --git a/foreman/data_refinery_foreman/surveyor/test_array_express.py b/foreman/data_refinery_foreman/surveyor/test_array_express.py index c1d41d3b9..47a2d971b 100644 --- a/foreman/data_refinery_foreman/surveyor/test_array_express.py +++ b/foreman/data_refinery_foreman/surveyor/test_array_express.py @@ -109,8 +109,7 @@ def test_survey_with_protocol_list(self): "/home/user/data_store/cassettes/surveyor.array_express.determine_accession.yaml" ) def test_determine_accession(self): - """Test of the `determine_sample_accession` function - """ + """Test of the `determine_sample_accession` function""" survey_job = self.create_job_for_accession("E-MTAB-3050") ae_surveyor = ArrayExpressSurveyor(survey_job) diff --git a/foreman/data_refinery_foreman/surveyor/test_geo.py b/foreman/data_refinery_foreman/surveyor/test_geo.py index 96037ea96..cd93b282f 100644 --- a/foreman/data_refinery_foreman/surveyor/test_geo.py +++ b/foreman/data_refinery_foreman/surveyor/test_geo.py @@ -34,7 +34,7 @@ def tearDown(self): @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.geo.geo_survey_microarray.yaml") @patch("data_refinery_foreman.surveyor.external_source.send_job") def test_geo_survey_microarray(self, mock_send_task): - """ Run the GEO surveyor and make sure we get some files to DL! + """Run the GEO surveyor and make sure we get some files to DL! For an Illumina Microarray platform. """ @@ -87,8 +87,7 @@ def test_geo_survey_GSE6532(self, mock_send_task): @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.geo.geo_survey_not_agilent.yaml") @patch("data_refinery_foreman.surveyor.external_source.send_job") def test_geo_survey_not_agilent(self, mock_send_task): - """ Test to make sure we're setting MFG correctly - """ + """Test to make sure we're setting MFG correctly""" self.prep_test("GSE34198") geo_surveyor = GeoSurveyor(self.survey_job) @@ -100,7 +99,7 @@ def test_geo_survey_not_agilent(self, mock_send_task): @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.geo.geo_survey_agilent.yaml") @patch("data_refinery_foreman.surveyor.external_source.send_job") def test_geo_survey_agilent(self, mock_send_task): - """ Run the GEO surveyor and make sure we get some files to DL! + """Run the GEO surveyor and make sure we get some files to DL! For an Agilent Microarray platform. """ @@ -183,7 +182,7 @@ def test_geo_survey_superseries(self, mock_send_task): @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.geo.get_pubmet_id_title.yaml") def test_get_pubmed_id_title(self): - """ We scrape PMIDs now. """ + """We scrape PMIDs now.""" resp = get_title_and_authors_for_pubmed_id("22367537") self.assertEqual( resp[0], diff --git a/foreman/data_refinery_foreman/surveyor/test_harmony.py b/foreman/data_refinery_foreman/surveyor/test_harmony.py index 8e69068c4..7334cbfa5 100644 --- a/foreman/data_refinery_foreman/surveyor/test_harmony.py +++ b/foreman/data_refinery_foreman/surveyor/test_harmony.py @@ -36,7 +36,7 @@ def setUp(self): @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.harmony.sdrf_harmony.yaml") def test_sdrf_harmony(self): - """ Harmonize SDRF test""" + """Harmonize SDRF test""" metadata = parse_sdrf( "https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3050/E-MTAB-3050.sdrf.txt" @@ -56,7 +56,7 @@ def test_sdrf_harmony(self): @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.harmony.sdrf_big.yaml") @tag("slow") def test_sdrf_big(self): - """ Tests lots of different cases for harmonization""" + """Tests lots of different cases for harmonization""" lots = [ "E-GEOD-59071", diff --git a/foreman/data_refinery_foreman/surveyor/test_sra.py b/foreman/data_refinery_foreman/surveyor/test_sra.py index 3b4f9beda..0ec12f2b2 100644 --- a/foreman/data_refinery_foreman/surveyor/test_sra.py +++ b/foreman/data_refinery_foreman/surveyor/test_sra.py @@ -51,8 +51,7 @@ def tearDown(self): SurveyJob.objects.all().delete() def test_survey(self): - """A Simple test of the SRA surveyor. - """ + """A Simple test of the SRA surveyor.""" sra_surveyor = SraSurveyor(self.survey_job) sra_surveyor.discover_experiment_and_samples() @@ -69,8 +68,7 @@ def test_survey(self): @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.sra.srp_survey.yaml") @patch("data_refinery_foreman.surveyor.external_source.send_job") def test_srp_survey(self, mock_send_job): - """A slightly harder test of the SRA surveyor. - """ + """A slightly harder test of the SRA surveyor.""" survey_job = SurveyJob(source_type="SRA") survey_job.save() key_value_pair = SurveyJobKeyValue( @@ -148,8 +146,7 @@ def test_survey_unmated_reads(self, mock_send_job): @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.sra.survey_nonexistant.yaml") def test_nonexistant_srp_survey(self): - """Try surveying an accession that does not exist - """ + """Try surveying an accession that does not exist""" survey_job = SurveyJob(source_type="SRA") survey_job.save() key_value_pair = SurveyJobKeyValue( @@ -167,8 +164,7 @@ def test_nonexistant_srp_survey(self): "/home/user/data_store/cassettes/surveyor.sra.arrayexpress_alternate_accession.yaml" ) def test_arrayexpress_alternate_accession(self): - """ Make sure that ENA experiments correctly detect their ArrayExpress alternate accession - """ + """Make sure that ENA experiments correctly detect their ArrayExpress alternate accession""" survey_job = SurveyJob(source_type="SRA") survey_job.save() diff --git a/foreman/data_refinery_foreman/surveyor/test_transcriptome_index.py b/foreman/data_refinery_foreman/surveyor/test_transcriptome_index.py index c559419b9..4a90ff2a1 100644 --- a/foreman/data_refinery_foreman/surveyor/test_transcriptome_index.py +++ b/foreman/data_refinery_foreman/surveyor/test_transcriptome_index.py @@ -37,7 +37,7 @@ def test_survey(self, mock_send_job): "/home/user/data_store/cassettes/surveyor.transcriptome.correct_index_location.yaml" ) def test_correct_index_location(self): - """ Tests that the files returned actually exist. + """Tests that the files returned actually exist. Uses an organism in the main division. """ @@ -68,7 +68,7 @@ def test_correct_index_location(self): "/home/user/data_store/cassettes/surveyor.transcriptome.correct_location_metazoa.yaml" ) def test_correct_index_location_metazoa(self): - """ Tests that the files returned actually exist. + """Tests that the files returned actually exist. Tests the Metazoa division instead of the main division. """ @@ -97,7 +97,7 @@ def test_correct_index_location_metazoa(self): @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.transcriptome.single_plant.yaml") def test_single_plant(self): - """ Tests that the files returned actually exist. + """Tests that the files returned actually exist. Tests the Metazoa division instead of the main division. """ @@ -128,7 +128,7 @@ def test_single_plant(self): "/home/user/data_store/cassettes/surveyor.transcriptome.correct_location_protist.yaml" ) def test_correct_index_location_protist(self): - """ Tests that the files returned actually exist. + """Tests that the files returned actually exist. Tests the Metazoa division instead of the main division. """ diff --git a/foreman/data_refinery_foreman/surveyor/transcriptome_index.py b/foreman/data_refinery_foreman/surveyor/transcriptome_index.py index cc84b37b9..d2b5df053 100644 --- a/foreman/data_refinery_foreman/surveyor/transcriptome_index.py +++ b/foreman/data_refinery_foreman/surveyor/transcriptome_index.py @@ -57,8 +57,7 @@ def get_strain_mapping_for_organism( species_name: str, config_file="config/organism_strain_mapping.csv" ) -> List[Dict]: - """Returns the row of the strain/organism mapping for the species_name - """ + """Returns the row of the strain/organism mapping for the species_name""" upper_name = species_name.upper() with open(config_file) as csvfile: reader = csv.DictReader(csvfile) @@ -369,7 +368,9 @@ def discover_species(self): ).value logger.info( - "Surveying %s division of ensembl.", ensembl_division, survey_job=self.survey_job.id, + "Surveying %s division of ensembl.", + ensembl_division, + survey_job=self.survey_job.id, ) try: diff --git a/foreman/data_refinery_foreman/surveyor/utils.py b/foreman/data_refinery_foreman/surveyor/utils.py index b8e44eb0f..d14c7d7df 100644 --- a/foreman/data_refinery_foreman/surveyor/utils.py +++ b/foreman/data_refinery_foreman/surveyor/utils.py @@ -44,7 +44,7 @@ def flatten(d, parent_key="", sep="_"): def get_title_and_authors_for_pubmed_id(pmid): - """ Given a PMID, return that PMID's (title, [authors]). """ + """Given a PMID, return that PMID's (title, [authors]).""" try: j_url = ( diff --git a/foreman/data_refinery_foreman/test_runner.py b/foreman/data_refinery_foreman/test_runner.py index 213d8639e..a6719a790 100644 --- a/foreman/data_refinery_foreman/test_runner.py +++ b/foreman/data_refinery_foreman/test_runner.py @@ -2,12 +2,12 @@ class NoDbTestRunner(DiscoverRunner): - """ A test runner to test without database creation """ + """A test runner to test without database creation""" def setup_databases(self, **kwargs): - """ Override the database creation defined in parent class """ + """Override the database creation defined in parent class""" pass def teardown_databases(self, old_config, **kwargs): - """ Override the database teardown defined in parent class """ + """Override the database teardown defined in parent class""" pass diff --git a/scripts/kill_all_jobs.py b/scripts/kill_all_jobs.py index 19f7db9ff..7e1853c2f 100644 --- a/scripts/kill_all_jobs.py +++ b/scripts/kill_all_jobs.py @@ -18,7 +18,9 @@ while "nextToken" in list_jobs_dict and list_jobs_dict["nextToken"]: list_jobs_dict = batch.list_jobs( - jobQueue=batch_queue_name, jobStatus=status, nextToken=list_jobs_dict["nextToken"], + jobQueue=batch_queue_name, + jobStatus=status, + nextToken=list_jobs_dict["nextToken"], ) for job in list_jobs_dict["jobSummaryList"]: diff --git a/scripts/run_full_pipeline.py b/scripts/run_full_pipeline.py index 9f49d618a..ba1b258d4 100755 --- a/scripts/run_full_pipeline.py +++ b/scripts/run_full_pipeline.py @@ -24,7 +24,8 @@ def parse_args(): def get_job_to_run(): completed_command = subprocess.run( - ["./foreman/run_management_command.sh", "get_job_to_be_run"], stdout=subprocess.PIPE, + ["./foreman/run_management_command.sh", "get_job_to_be_run"], + stdout=subprocess.PIPE, ) # The JSON output is on the last line of the output, but it has a diff --git a/workers/data_refinery_workers/downloaders/array_express.py b/workers/data_refinery_workers/downloaders/array_express.py index 6a69a921a..5b2a1fb4f 100644 --- a/workers/data_refinery_workers/downloaders/array_express.py +++ b/workers/data_refinery_workers/downloaders/array_express.py @@ -27,8 +27,8 @@ def _download_file(download_url: str, file_path: str, job: DownloaderJob) -> None: - """ Download a file from ArrayExpress via FTP. There is no Aspera endpoint - which I can find. """ + """Download a file from ArrayExpress via FTP. There is no Aspera endpoint + which I can find.""" try: logger.debug( "Downloading file from %s to %s.", download_url, file_path, downloader_job=job.id @@ -45,8 +45,7 @@ def _download_file(download_url: str, file_path: str, job: DownloaderJob) -> Non def _extract_files(file_path: str, accession_code: str, job: DownloaderJob) -> List[str]: - """Extract zip and return a list of the raw files. - """ + """Extract zip and return a list of the raw files.""" logger.debug("Extracting %s!", file_path, file_path=file_path, downloader_job=job.id) abs_with_code_raw = LOCAL_ROOT_DIR + "/" + accession_code + "/raw/" diff --git a/workers/data_refinery_workers/downloaders/geo.py b/workers/data_refinery_workers/downloaders/geo.py index 29f37f6ab..d395c5f2c 100644 --- a/workers/data_refinery_workers/downloaders/geo.py +++ b/workers/data_refinery_workers/downloaders/geo.py @@ -27,8 +27,7 @@ def _download_file(download_url: str, file_path: str, job: DownloaderJob, force_ftp=False) -> None: - """ Download a file from GEO via Aspera unless `force_ftp` is True - """ + """Download a file from GEO via Aspera unless `force_ftp` is True""" # Ensure directory exists os.makedirs(file_path.rsplit("/", 1)[0], exist_ok=True) @@ -64,7 +63,7 @@ def _download_file(download_url: str, file_path: str, job: DownloaderJob, force_ def _download_file_aspera( download_url: str, downloader_job: DownloaderJob, target_file_path: str, attempt=0 ) -> bool: - """ Download a file to a location using Aspera by shelling out to the `ascp` client. """ + """Download a file to a location using Aspera by shelling out to the `ascp` client.""" try: logger.debug( @@ -154,7 +153,7 @@ def __init__(self, file_path, original_error): class ArchivedFile: - """ Contains utility functions to enumerate the files inside an archive that was downloaded from GEO. """ + """Contains utility functions to enumerate the files inside an archive that was downloaded from GEO.""" def __init__(self, downloaded_file_path, parent_archive=None): self.file_path = downloaded_file_path @@ -165,31 +164,31 @@ def __init__(self, downloaded_file_path, parent_archive=None): self.extension = os.path.splitext(self.file_path)[1] def parent_is_archive(self): - """ Returns true if this file came from an archive """ + """Returns true if this file came from an archive""" return self.parent_archive and self.parent_archive.is_archive() def experiment_accession_code(self): - """ Tries to get an experiment accession code from the file name. - GEO experiment accession codes start with GSE and have between 5 and 9 characters """ + """Tries to get an experiment accession code from the file name. + GEO experiment accession codes start with GSE and have between 5 and 9 characters""" match = re.match(r"(GSE\d{2,6})", self.filename) if match: return match.group(0) return None def sample_accession_code(self): - """ Tries to get a sample accession code from the file name. - GEO sample accession codes start with GSM and have between 6 and 10 characters """ + """Tries to get a sample accession code from the file name. + GEO sample accession codes start with GSM and have between 6 and 10 characters""" match = re.match(r"(GSM\d{4,7})", self.filename) if match: return match.group(0) return None def get_sample(self): - """ Tries to find the sample associated with this file, and returns None if unable. """ + """Tries to find the sample associated with this file, and returns None if unable.""" return Sample.objects.filter(accession_code=self.sample_accession_code()).first() def is_processable(self): - """ There're some known file patterns that are found in GEO that we know we can ignore. """ + """There're some known file patterns that are found in GEO that we know we can ignore.""" if re.match(r"(GSE\d{2,6})_family.xml", self.filename): return False @@ -234,7 +233,7 @@ def _extract_files(self): raise FileExtractionError(self.file_path, "Unknown archive file format.") def _get_absolute_path(self): - """ This returns the path where this file would be extracted if it's an archive """ + """This returns the path where this file would be extracted if it's an archive""" if self.experiment_accession_code(): return LOCAL_ROOT_DIR + "/" + self.experiment_accession_code() + "/raw/" elif self.sample_accession_code(): @@ -243,7 +242,7 @@ def _get_absolute_path(self): return LOCAL_ROOT_DIR + "/" + self.filename + "/raw/" def _extract_tar(self) -> List[str]: - """ Extract tar and return a list of the raw files. """ + """Extract tar and return a list of the raw files.""" # This is technically an unsafe operation. # However, we're trusting GEO as a data source. abs_with_code_raw = self._get_absolute_path() diff --git a/workers/data_refinery_workers/downloaders/sra.py b/workers/data_refinery_workers/downloaders/sra.py index 181135027..c4a6a367b 100644 --- a/workers/data_refinery_workers/downloaders/sra.py +++ b/workers/data_refinery_workers/downloaders/sra.py @@ -29,8 +29,7 @@ def _download_file( original_file: OriginalFile, downloader_job: DownloaderJob, target_file_path: str ) -> bool: - """ Download file dispatcher. Dispatches to the HTTP or Aspera downloader - """ + """Download file dispatcher. Dispatches to the HTTP or Aspera downloader""" download_url = original_file.source_url # SRA files have Apsera downloads. if "ftp.sra.ebi.ac.uk" in download_url: @@ -92,7 +91,7 @@ def _download_file_aspera( original_file=None, source="NCBI", ) -> bool: - """ Download a file to a location using Aspera by shelling out to the `ascp` client. """ + """Download a file to a location using Aspera by shelling out to the `ascp` client.""" try: logger.debug( diff --git a/workers/data_refinery_workers/downloaders/test_geo.py b/workers/data_refinery_workers/downloaders/test_geo.py index 9d951a33b..8f6ebc2de 100644 --- a/workers/data_refinery_workers/downloaders/test_geo.py +++ b/workers/data_refinery_workers/downloaders/test_geo.py @@ -90,7 +90,7 @@ def test_download_and_extract_file(self): @tag("downloaders") def test_download_aspera_and_ftp(self): - """ Tests the main 'download_geo' function. """ + """Tests the main 'download_geo' function.""" dlj = DownloaderJob() dlj.accession_code = "GSE22427" @@ -158,7 +158,7 @@ def test_download_aspera_and_ftp(self): @tag("downloaders") def test_download_geo(self): - """ Tests the main 'download_geo' function. """ + """Tests the main 'download_geo' function.""" dlj = DownloaderJob() dlj.accession_code = "GSE22427" @@ -217,8 +217,7 @@ def test_download_geo(self): @tag("downloaders") def test_no_rnaseq(self): - """Makes sure that no RNA-Seq data gets downloaded even if there's a job for it. - """ + """Makes sure that no RNA-Seq data gets downloaded even if there's a job for it.""" dlj = DownloaderJob() dlj.accession_code = "GSE103217" dlj.save() diff --git a/workers/data_refinery_workers/downloaders/transcriptome_index.py b/workers/data_refinery_workers/downloaders/transcriptome_index.py index a09999166..1ffb005f6 100644 --- a/workers/data_refinery_workers/downloaders/transcriptome_index.py +++ b/workers/data_refinery_workers/downloaders/transcriptome_index.py @@ -129,7 +129,7 @@ def download_transcriptome(job_id: int) -> None: def create_long_and_short_processor_jobs( downloader_job, long_files_to_process, short_files_to_process ): - """ Creates two processor jobs for the files needed for this transcriptome""" + """Creates two processor jobs for the files needed for this transcriptome""" processor_job_long = ProcessorJob() processor_job_long.downloader_job = downloader_job diff --git a/workers/data_refinery_workers/processors/create_quantpendia.py b/workers/data_refinery_workers/processors/create_quantpendia.py index 7dbdd8654..3336409e4 100644 --- a/workers/data_refinery_workers/processors/create_quantpendia.py +++ b/workers/data_refinery_workers/processors/create_quantpendia.py @@ -196,8 +196,8 @@ def _create_result_objects(job_context: Dict) -> Dict: def _remove_job_dir(job_context: Dict): - """ remove the directory when the job is successful. At this point - the quantpendia was already zipped and uploaded. """ + """remove the directory when the job is successful. At this point + the quantpendia was already zipped and uploaded.""" # don't remove the files when running locally or for tests if settings.RUNNING_IN_CLOUD: shutil.rmtree(job_context["job_dir"], ignore_errors=True) diff --git a/workers/data_refinery_workers/processors/illumina.py b/workers/data_refinery_workers/processors/illumina.py index e7d1cc368..c216ef584 100644 --- a/workers/data_refinery_workers/processors/illumina.py +++ b/workers/data_refinery_workers/processors/illumina.py @@ -73,7 +73,12 @@ def _detect_encoding(job_context: Dict) -> Dict: try: encoding = subprocess.check_output( - ["file", "--brief", "--mime-encoding", job_context["input_file_path"],], + [ + "file", + "--brief", + "--mime-encoding", + job_context["input_file_path"], + ], encoding="utf-8", ).strip() except subprocess.CalledProcessError as e: @@ -365,7 +370,11 @@ def _detect_platform(job_context: Dict) -> Dict: "illuminaHumanv3", "illuminaHumanv4", ], - "MUS_MUSCULUS": ["illuminaMousev1", "illuminaMousev1p1", "illuminaMousev2",], + "MUS_MUSCULUS": [ + "illuminaMousev1", + "illuminaMousev1p1", + "illuminaMousev2", + ], "RATTUS_NORVEGICUS": ["illuminaRatv1"], } diff --git a/workers/data_refinery_workers/processors/janitor.py b/workers/data_refinery_workers/processors/janitor.py index 1d5fa12b6..83b368901 100644 --- a/workers/data_refinery_workers/processors/janitor.py +++ b/workers/data_refinery_workers/processors/janitor.py @@ -23,7 +23,7 @@ def _find_and_remove_expired_jobs(job_context): - """ Finds expired jobs and removes their working directories """ + """Finds expired jobs and removes their working directories""" job_context["deleted_items"] = [] diff --git a/workers/data_refinery_workers/processors/management/commands/fetch_compendia.py b/workers/data_refinery_workers/processors/management/commands/fetch_compendia.py index c2fa3f6e3..2af837764 100644 --- a/workers/data_refinery_workers/processors/management/commands/fetch_compendia.py +++ b/workers/data_refinery_workers/processors/management/commands/fetch_compendia.py @@ -15,8 +15,7 @@ def add_arguments(self, parser): parser.add_argument("--dataset-ids", type=str, help=("Comma-separated Dataset IDs.")) def handle(self, *args, **options): - """ Given a dataset ID, fetch the resulting smashed object. - """ + """Given a dataset ID, fetch the resulting smashed object.""" if options["dataset_ids"] is None: logger.error("You must specify dataset IDs.") diff --git a/workers/data_refinery_workers/processors/management/commands/qn_dispatcher.py b/workers/data_refinery_workers/processors/management/commands/qn_dispatcher.py index ff5b03b71..829088c54 100644 --- a/workers/data_refinery_workers/processors/management/commands/qn_dispatcher.py +++ b/workers/data_refinery_workers/processors/management/commands/qn_dispatcher.py @@ -19,7 +19,7 @@ class Command(BaseCommand): def handle(self, *args, **options): - """ Dispatch QN_REFERENCE creation jobs for all Organisms with a platform with enough processed samples. """ + """Dispatch QN_REFERENCE creation jobs for all Organisms with a platform with enough processed samples.""" organisms = Organism.objects.all() diff --git a/workers/data_refinery_workers/processors/management/commands/remove_invalid_computational_results.py b/workers/data_refinery_workers/processors/management/commands/remove_invalid_computational_results.py index 614985f73..0d13b7cbf 100644 --- a/workers/data_refinery_workers/processors/management/commands/remove_invalid_computational_results.py +++ b/workers/data_refinery_workers/processors/management/commands/remove_invalid_computational_results.py @@ -35,7 +35,7 @@ def add_arguments(self, parser): ) def handle(self, *args, **options): - """ Takes care of removing invalid computational results. """ + """Takes care of removing invalid computational results.""" computational_result_ids = [] if options["compendias"]: diff --git a/workers/data_refinery_workers/processors/management/commands/run_tximport.py b/workers/data_refinery_workers/processors/management/commands/run_tximport.py index b491667e7..38b736f77 100644 --- a/workers/data_refinery_workers/processors/management/commands/run_tximport.py +++ b/workers/data_refinery_workers/processors/management/commands/run_tximport.py @@ -22,8 +22,7 @@ def add_arguments(self, parser): ) def handle(self, *args, **options): - """Run tximport on an experiment for the samples that have been processed by Salmon. - """ + """Run tximport on an experiment for the samples that have been processed by Salmon.""" if options["experiment_accession"] is None: logger.error("The --experiment-accession argument must be provided") sys.exit(1) diff --git a/workers/data_refinery_workers/processors/no_op.py b/workers/data_refinery_workers/processors/no_op.py index 636d99be8..bc37935a3 100644 --- a/workers/data_refinery_workers/processors/no_op.py +++ b/workers/data_refinery_workers/processors/no_op.py @@ -22,8 +22,7 @@ def _prepare_files(job_context: Dict) -> Dict: - """A processor which takes externally-processed sample data and makes it smashable. - """ + """A processor which takes externally-processed sample data and makes it smashable.""" try: original_file = job_context["original_files"][0] sample0 = job_context["samples"][0] @@ -167,7 +166,7 @@ def _prepare_files(job_context: Dict) -> Dict: def _convert_genes(job_context: Dict) -> Dict: - """ Dispatches to the appropriate gene converter""" + """Dispatches to the appropriate gene converter""" sample0 = job_context["samples"][0] if sample0.manufacturer == "ILLUMINA": @@ -177,7 +176,7 @@ def _convert_genes(job_context: Dict) -> Dict: def _convert_affy_genes(job_context: Dict) -> Dict: - """ Convert to Ensembl genes if we can""" + """Convert to Ensembl genes if we can""" if "internal_accession" not in job_context or not job_context["internal_accession"]: error_msg = ( @@ -258,7 +257,7 @@ def _convert_affy_genes(job_context: Dict) -> Dict: def _convert_illumina_genes(job_context: Dict) -> Dict: - """ Convert to Ensembl genes if we can""" + """Convert to Ensembl genes if we can""" all_databases = { "HOMO_SAPIENS": [ @@ -267,7 +266,11 @@ def _convert_illumina_genes(job_context: Dict) -> Dict: "illuminaHumanv3", "illuminaHumanv4", ], - "MUS_MUSCULUS": ["illuminaMousev1", "illuminaMousev1p1", "illuminaMousev2",], + "MUS_MUSCULUS": [ + "illuminaMousev1", + "illuminaMousev1p1", + "illuminaMousev2", + ], "RATTUS_NORVEGICUS": ["illuminaRatv1"], } @@ -362,7 +365,7 @@ def _convert_illumina_genes(job_context: Dict) -> Dict: def _create_result(job_context: Dict) -> Dict: - """ Create the actual Result object""" + """Create the actual Result object""" # This is a NO-OP, but we make a ComputationalResult regardless. result = ComputationalResult() @@ -408,7 +411,7 @@ def _create_result(job_context: Dict) -> Dict: def check_output_quality(output_file_path: str): - """ Verify our output file meets spec """ + """Verify our output file meets spec""" try: with open(output_file_path, "r") as tsv_in: tsv_in = csv.reader(tsv_in, delimiter="\t") diff --git a/workers/data_refinery_workers/processors/smasher.py b/workers/data_refinery_workers/processors/smasher.py index da9c5c485..fa832925f 100644 --- a/workers/data_refinery_workers/processors/smasher.py +++ b/workers/data_refinery_workers/processors/smasher.py @@ -327,7 +327,9 @@ def _upload(job_context: Dict) -> Dict: # Note that file expiry is handled by the S3 object lifecycle, # managed by terraform. s3_client.upload_file( - job_context["output_file"], RESULTS_BUCKET, output_filename, + job_context["output_file"], + RESULTS_BUCKET, + output_filename, ) except Exception: raise utils.ProcessorJobError( @@ -484,13 +486,29 @@ def _notify_send_email(job_context): # Provide the contents of the email. client.send_email( - Destination={"ToAddresses": [RECIPIENT,], "BccAddresses": [SLACK_CCDL_EMAIL,],}, + Destination={ + "ToAddresses": [ + RECIPIENT, + ], + "BccAddresses": [ + SLACK_CCDL_EMAIL, + ], + }, Message={ "Body": { - "Html": {"Charset": CHARSET, "Data": FORMATTED_HTML,}, - "Text": {"Charset": CHARSET, "Data": BODY_TEXT,}, + "Html": { + "Charset": CHARSET, + "Data": FORMATTED_HTML, + }, + "Text": { + "Charset": CHARSET, + "Data": BODY_TEXT, + }, + }, + "Subject": { + "Charset": CHARSET, + "Data": SUBJECT, }, - "Subject": {"Charset": CHARSET, "Data": SUBJECT,}, }, Source=SENDER, ) diff --git a/workers/data_refinery_workers/processors/test_illumina.py b/workers/data_refinery_workers/processors/test_illumina.py index de2ff9d0e..7bccadecd 100644 --- a/workers/data_refinery_workers/processors/test_illumina.py +++ b/workers/data_refinery_workers/processors/test_illumina.py @@ -318,7 +318,10 @@ def test_illumina_quoted_row_names(self): "filename": "GSE33814_trimmed_non-normalized.txt", "absolute_file_path": "/home/user/data_store/raw/TEST/ILLUMINA/GSE33814_trimmed_non-normalized.txt", "organism": organism, - "samples": [("GSM836222", "IMGUS_32"), ("GSM836223", "IMGUS_33"),], + "samples": [ + ("GSM836222", "IMGUS_32"), + ("GSM836223", "IMGUS_33"), + ], } ) @@ -357,12 +360,16 @@ def test_illumina_rows_starting_with_whitespace(self): ( "GSM3071991", "MCF-7 KLHDC7B siRNA knockdown control", - {"description": ["SAMPLE 1"],}, + { + "description": ["SAMPLE 1"], + }, ), ( "GSM3071992", "MCF-7 KLHDC7B siRNA knockdown", - {"description": ["SAMPLE 2"],}, + { + "description": ["SAMPLE 2"], + }, ), ], } @@ -420,7 +427,9 @@ def test_illumina_no_pvalue(self): "filename": "GSE41355_non-normalized.txt", "absolute_file_path": "/home/user/data_store/raw/TEST/ILLUMINA/GSE41355_non-normalized.txt", "organism": organism, - "samples": [("GSM1015436", "IRF3/7 DKO 2"),], + "samples": [ + ("GSM1015436", "IRF3/7 DKO 2"), + ], } ) @@ -445,7 +454,13 @@ def test_illumina_id_ref_column_with_whitespace(self): "absolute_file_path": "/home/user/data_store/raw/TEST/ILLUMINA/GSE100301_non-normalized.txt", "organism": organism, "samples": [ - ("GSM2677583", "22Rv1-tetO-Gal4, replicate 1", {"description": ["SAMPLE 1"],},), + ( + "GSM2677583", + "22Rv1-tetO-Gal4, replicate 1", + { + "description": ["SAMPLE 1"], + }, + ), ], } ) diff --git a/workers/data_refinery_workers/processors/test_janitor.py b/workers/data_refinery_workers/processors/test_janitor.py index a3ba2aa3a..fb755ca8e 100644 --- a/workers/data_refinery_workers/processors/test_janitor.py +++ b/workers/data_refinery_workers/processors/test_janitor.py @@ -79,7 +79,7 @@ class JanitorTestCase(TestCase): @tag("janitor") @patch("data_refinery_workers.processors.janitor.batch.describe_jobs") def test_janitor(self, mock_describe_jobs): - """ Main tester. """ + """Main tester.""" job = prepare_job() mock_describe_jobs.return_value = {"jobs": [{"jobId": "running_job", "status": "RUNNING"}]} diff --git a/workers/data_refinery_workers/processors/test_salmon.py b/workers/data_refinery_workers/processors/test_salmon.py index afe00ca03..ff390915c 100644 --- a/workers/data_refinery_workers/processors/test_salmon.py +++ b/workers/data_refinery_workers/processors/test_salmon.py @@ -496,8 +496,7 @@ def test_salmon_quant_two_samples_single_read(self): @tag("salmon") def test_set_tximport_inputs(self): - """"Tests that tximport only considers RNA-Seq samples from GEO. - """ + """ "Tests that tximport only considers RNA-Seq samples from GEO.""" # Create one experiment and two related samples, based on: # https://www.ncbi.nlm.nih.gov/sra/?term=SRP040623 # (We don't need any original files because @@ -1070,8 +1069,7 @@ def test_early_tximport(self): @tag("salmon") def test_version_filter(self): - """Tests that we don't run tximport on old salmon versions. - """ + """Tests that we don't run tximport on old salmon versions.""" # Accessions SRR5125616-SRR5125620 don't exist in SRA, but we # don't actually want to process them so it's okay. incomplete_accessions = [ diff --git a/workers/data_refinery_workers/processors/test_transcriptome_index.py b/workers/data_refinery_workers/processors/test_transcriptome_index.py index fb1187b73..c549afa5f 100644 --- a/workers/data_refinery_workers/processors/test_transcriptome_index.py +++ b/workers/data_refinery_workers/processors/test_transcriptome_index.py @@ -121,7 +121,10 @@ def test_process_gtf(self): job_context = transcriptome_index._process_gtf(job_context) transcript_to_gene_ids = pd.read_csv( - job_context["genes_to_transcripts_path"], sep="\t", index_col=1, names=["Gene"], + job_context["genes_to_transcripts_path"], + sep="\t", + index_col=1, + names=["Gene"], )["Gene"] self.assertEqual(transcript_to_gene_ids.get("ENST00000480647", None), "ENSG00000041988") diff --git a/workers/data_refinery_workers/processors/tximport.py b/workers/data_refinery_workers/processors/tximport.py index 7395815c2..9df86c0f0 100644 --- a/workers/data_refinery_workers/processors/tximport.py +++ b/workers/data_refinery_workers/processors/tximport.py @@ -20,14 +20,13 @@ def _set_job_prefix(job_context: Dict) -> Dict: - """ Sets the `job_dir_prefix` value in the job context object.""" + """Sets the `job_dir_prefix` value in the job context object.""" job_context["job_dir_prefix"] = JOB_DIR_PREFIX + str(job_context["job_id"]) return job_context def _prepare_files(job_context: Dict) -> Dict: - """Moves the file(s) from the raw directory to the temp directory. - """ + """Moves the file(s) from the raw directory to the temp directory.""" logger.debug("Preparing files..") # Create a directory specific to this processor job. From f1b1c06ee75d91a97fa40c2276c3d7b937431a72 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 8 Sep 2022 18:12:39 -0700 Subject: [PATCH 012/220] Port Python script to Django command. - Introduce AccessionBacklogEntry model. - Clean up command flags. - Get previous accessions from the DB. - --- .../migrations/0071_accessionbacklogentry.py | 38 + .../data_refinery_common/models/accession.py | 82 +- .../gatherer/__init__.py | 0 .../gatherer/management/__init__.py | 0 .../gatherer/management/commands/__init__.py | 0 .../management/commands/gather_accessions.py | 731 ++++++++++++++++++ foreman/data_refinery_foreman/settings.py | 17 +- foreman/dockerfiles/Dockerfile.foreman | 2 + 8 files changed, 856 insertions(+), 14 deletions(-) create mode 100644 common/data_refinery_common/migrations/0071_accessionbacklogentry.py create mode 100644 foreman/data_refinery_foreman/gatherer/__init__.py create mode 100644 foreman/data_refinery_foreman/gatherer/management/__init__.py create mode 100644 foreman/data_refinery_foreman/gatherer/management/commands/__init__.py create mode 100644 foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py diff --git a/common/data_refinery_common/migrations/0071_accessionbacklogentry.py b/common/data_refinery_common/migrations/0071_accessionbacklogentry.py new file mode 100644 index 000000000..86c04daed --- /dev/null +++ b/common/data_refinery_common/migrations/0071_accessionbacklogentry.py @@ -0,0 +1,38 @@ +# Generated by Django 3.2.7 on 2022-09-07 19:31 + +from django.db import migrations, models + + +class Migration(migrations.Migration): + + dependencies = [ + ("data_refinery_common", "0070_auto_20211208_2118"), + ] + + operations = [ + migrations.CreateModel( + name="AccessionBacklogEntry", + fields=[ + ( + "id", + models.AutoField( + auto_created=True, + primary_key=True, + serialize=False, + verbose_name="ID", + ), + ), + ("code", models.TextField(unique=True)), + ("created_at", models.DateTimeField(auto_now_add=True)), + ("last_modified_at", models.DateTimeField(auto_now=True)), + ("organism", models.TextField()), + ("published_date", models.DateTimeField()), + ("sample_count", models.PositiveIntegerField(default=0)), + ("source", models.TextField()), + ("technology", models.TextField()), + ], + options={ + "db_table": "accession_backlog", + }, + ), + ] diff --git a/common/data_refinery_common/models/accession.py b/common/data_refinery_common/models/accession.py index dc93cfd88..6ac62da9f 100644 --- a/common/data_refinery_common/models/accession.py +++ b/common/data_refinery_common/models/accession.py @@ -1,18 +1,16 @@ +from datetime import datetime + from django.db import models +from django.utils import timezone -class Accession(models.Model): - """Accession model.""" +class AccessionBacklogEntry(models.Model): + """Accession backlog entry model.""" class Meta: - constraints = ( - models.UniqueConstraint( - fields=("code", "source", "technology"), name="unique_accession" - ), - ) - db_table = "accessions" - - code = models.TextField() + db_table = "accession_backlog" + + code = models.TextField(unique=True) created_at = models.DateTimeField(auto_now_add=True) last_modified_at = models.DateTimeField(auto_now=True) organism = models.TextField() @@ -20,3 +18,67 @@ class Meta: sample_count = models.PositiveIntegerField(default=0) source = models.TextField() technology = models.TextField() + + def __eq__(self, other: object) -> bool: + """Returns True if two objects are equal. Otherwise returns False.""" + return isinstance(other, AccessionBacklogEntry) and self.code == other.code + + def __hash__(self) -> int: + """Returns accession object unique hash value.""" + return hash(self.code) + + def __str__(self) -> str: + """Returns accession default string representation.""" + return ", ".join((self.code, self.technology, self.source, str(self.published_date.date()))) + + @staticmethod + def create_from_ma_ae_entry(entry): + """Creates accession object from MicroArray ArrayExpress entry.""" + accession = AccessionBacklogEntry() + accession.code = entry["accession"] + accession.source = "ebi_biostudies" + accession.technology = "microarray" + + if "organism" in entry: + accession.organism = entry["organism"] + if "release_date" in entry: + accession.published_date = timezone.make_aware( + datetime.strptime(entry["release_date"], "%Y-%m-%d") + ) + + return accession + + @staticmethod + def create_from_ma_geo_entry(entry): + """Creates accession object from MicroArray GEO meta DB entry.""" + accession = AccessionBacklogEntry() + accession.code = entry["gse"] + accession.source = "geo_meta_db" + accession.technology = "microarray" + + if "organism" in entry: + accession.organism = entry["organism"].lower() + if "submission_date" in entry: + + accession.published_date = timezone.make_aware( + datetime.strptime(entry["submission_date"], "%Y-%m-%d") + ) + + return accession + + @staticmethod + def create_from_rnaseq_entry(entry): + """Creates accession object from RNA-Seq entry.""" + accession = AccessionBacklogEntry() + accession.code = entry["secondary_study_accession"] + accession.source = "ebi_ena_portal" + accession.technology = "rna-seq" + + if "scientific_name" in entry: + accession.organism = entry["scientific_name"].lower() + if "first_public" in entry: + accession.published_date = timezone.make_aware( + datetime.strptime(entry["first_public"], "%Y-%m-%d") + ) + + return accession diff --git a/foreman/data_refinery_foreman/gatherer/__init__.py b/foreman/data_refinery_foreman/gatherer/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/gatherer/management/__init__.py b/foreman/data_refinery_foreman/gatherer/management/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/__init__.py b/foreman/data_refinery_foreman/gatherer/management/commands/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py new file mode 100644 index 000000000..c4808a191 --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -0,0 +1,731 @@ +"""MicroArray (ArrayExpress, GEO) and RNA-Seq accession gathering automation. +Data sources: + - https://www.ebi.ac.uk/biostudies/help (MicroArray ArrayExpress). + - local SQLite meta DB from https://www.bioconductor.org/packages/release/bioc/html/GEOmetadb.html + (MicroArray GEO). + - https://www.ebi.ac.uk/ena/portal/api/ (RNA-Seq). +""" + +import argparse +import logging +import os +import re +import sqlite3 +from datetime import datetime +from http.client import RemoteDisconnected +from json.decoder import JSONDecodeError +from typing import List, Set +from urllib.parse import quote + +from django.core.management.base import BaseCommand + +import requests +from requests.exceptions import ConnectionError, ConnectTimeout +from retrying import retry +from urllib3.exceptions import ProtocolError + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.accession import AccessionBacklogEntry +from data_refinery_common.models.experiment import Experiment + +log = get_and_configure_logger(__name__) + + +class Command(BaseCommand): + """Creates agents and runs actual accession gathering.""" + + RE_ACCESSION = re.compile(r"(\D+)(\d+)") + RE_DATE = re.compile(r"\d{4}-\d{2}-\d{2}") + + # TODO(ark): remove after upgrade to python3.8 where parser argument + # "extend" action is directly available. + # https://docs.python.org/3.8/library/argparse.html#action + class ExtendAction(argparse.Action): + def __call__(self, parser, namespace, values, option_string=None): + items = getattr(namespace, self.dest) or [] + items.extend(values) + setattr(namespace, self.dest, items) + + def add_arguments(self, parser) -> None: + parser.register("action", "extend", Command.ExtendAction) + + parser.add_argument( + "--ae-id", + action="extend", + nargs="+", + type=str, + help="ArrayExpress ID(s) to use for filtering.", + ) + parser.add_argument( + "--ae-ids-file", + type=str, + help="Path to a file containing ArrayExpress ID(s) to use for filtering.", + ) + parser.add_argument("-c", "--count", type=int, help="Number of accessions to collect.") + parser.add_argument( + "-d", + "--dry-run", + action="store_true", + default=False, + help="Do not write the result to the database.", + ) + parser.add_argument( + "-e", + "--exclude-previous", + action="store_true", + default=True, + help="Exclude previously gathered or surveyed accessions.", + ) + parser.add_argument( + "-ne", + "--no-exclude-previous", + action="store_false", + default=False, + dest="exclude_previous", + help="Do not exclude previously gathered or surveyed accessions.", + ) + parser.add_argument( + "--gpl-id", + action="extend", + nargs="+", + type=str, + help="GEO platform ID(s) to use for filtering.", + ) + parser.add_argument( + "--gpl-ids-file", + type=str, + help="Path to a file containing GEO platform ID(s) to use for filtering.", + ) + parser.add_argument( + "-k", + "--keyword", + type=str, + help="Keyword to use for filtering.", + ) + parser.add_argument( + "-m", + "--microarray", + action="store_true", + default=False, + help="Collect MicroArray accessions.", + ) + parser.add_argument( + "-o", "--organism", type=str, help="Organism name to use for filtering." + ) + parser.add_argument( + "-r", + "--rna-seq", + action="store_true", + default=False, + help="Collect RNA-Seq accessions.", + ) + parser.add_argument( + "-s", + "--since", + type=str, + required=True, + help="Collect accessions made public on or after this date.", + ) + parser.add_argument( + "--taxon-id", + action="extend", + nargs="+", + type=int, + help="Taxon ID(s) to use for filtering.", + ) + parser.add_argument( + "--taxon-ids-file", + type=str, + help="Path to a file containing taxon ID(s) to use for filtering.", + ) + parser.add_argument( + "-u", + "--until", + type=str, + help="Collect accessions made public before or on this date.", + ) + parser.add_argument( + "-lv", + "--log-verbose", + action="store_true", + default=False, + help="Enable verbose log output.", + ) + + def set_verbosity_level(self, options) -> None: + """Configures log verbosity level.""" + if options["log_verbose"]: + log.addHandler(logging.StreamHandler()) + log.setLevel(logging.DEBUG) + else: + log.setLevel(logging.ERROR) + + def validate_args(self, options) -> None: + """Validates arguments.""" + if not options["microarray"] and not options["rna_seq"]: + exit("Either --microarray or --rna-seq must be specified.") + + errors = list() + since = options["since"] + until = options["until"] + if not self.RE_DATE.match(since): + errors.append('The -s, --since value must match "YYYY-MM-DD" format.') + if until and not self.RE_DATE.match(until): + errors.append('The -u, --until value must match "YYYY-MM-DD" format.') + if since and until and since > until: + errors.append("The -s, --since date must be earlier than -u, --until date.") + + keyword = options["keyword"] + organism = options["organism"] + if options["microarray"]: + ae_id = options["ae_id"] or options["ae_ids_file"] + gpl_id = options["gpl_id"] or options["gpl_ids_file"] + ids = ae_id or gpl_id + invalid_options_message = ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " + "ArrayExpress ID(s) [--ae-id, --ae-ids-file] / GEO platform ID(s) " + "[--gpl-id, --gpl-ids-file] must be specified." + ) + elif options["rna_seq"]: + taxon_id = options["taxon_id"] or options["taxon_ids_file"] + ids = taxon_id + invalid_options_message = ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] " + "or taxon ID(s) [--taxon-id, --taxon-ids-file] must be specified." + ) + + if len([option for option in (ids, keyword, organism) if option]) != 1: + errors.append(invalid_options_message) + + if errors: + exit("\n".join(errors)) + + def handle(self, *args, **options): + """Runs the accession gathering process.""" + self.validate_args(options) + self.set_verbosity_level(options) + + agents = list() + if options["rna_seq"]: + agents.append(RNASeqAccessionAgent(options)) + elif options["microarray"]: + if ( + options["ae_id"] + or options["ae_ids_file"] + or options["keyword"] + or options["organism"] + ): + agents.append(MicroArrayExpressAccessionAgent(options)) + if ( + options["gpl_id"] + or options["gpl_ids_file"] + or options["keyword"] + or options["organism"] + ): + agents.append(MicroArrayGEOAccessionAgent(options)) + + entries = set() + for agent in agents: + entries.update(agent.collect_data()) + + entries = sorted( # Sort the resulting list. + (entry for entry in entries if self.RE_ACCESSION.match(entry.code)), + key=lambda entry: ( + self.RE_ACCESSION.match(entry.code).group(1), + int(self.RE_ACCESSION.match(entry.code).group(2)), + ), + ) + # Limit the number of output entries. + entries = entries[: options["count"]] if options["count"] else entries + + if options["dry_run"]: + if entries: + output = "\n".join((str(entry) for entry in entries)) + else: + output = "No accessions found." + print(output) + else: + AccessionBacklogEntry.objects.bulk_create(entries) + + +class AccessionAgentBase: + "Accession agent base class." + + previous_accessions = set() + retry_params = { + "retry_on_exception": lambda e: isinstance( + e, (ConnectionError, ConnectTimeout, ProtocolError, RemoteDisconnected) + ), + "stop_max_attempt_number": 5, + "wait_exponential_multiplier": 1000, # Seconds. + "wait_exponential_max": 16000, # Seconds. + } + + def __init__(self, options) -> None: + """Populates args and values for major variables.""" + self.options = options + self.count = options["count"] + self.keyword = options["keyword"] + self.organism = options["organism"] + self.since = options["since"] + self.until = options["until"] or datetime.now().strftime("%Y-%m-%d") + + self.populate_previous_accessions() + + def build_query(self): + """Returns query/query dict depending on the accession data source.""" + raise NotImplementedError + + def collect_data(self): + """Generates resulting entry collection.""" + raise NotImplementedError + + def fetch_data(self): + """Fetches data from an external or local data source.""" + raise NotImplementedError + + def get_ids(self): + """Gets IDs for query filtering depending on the accession technology.""" + raise NotImplementedError + + def populate_previous_accessions(self) -> None: + """Populates previous accession set from a provided excluded ids file.""" + if not self.options["exclude_previous"] or self.previous_accessions: + return + + # Gathered accessions. + self.previous_accessions.update( + (entry["code"] for entry in AccessionBacklogEntry.objects.values("code")) + ) + + # Surveyed accessions. + experiments = Experiment.objects.values("accession_code", "alternate_accession_code") + self.previous_accessions.update( + (experiment["accession_code"] for experiment in experiments) + ) + self.previous_accessions.update( + (experiment["alternate_accession_code"] for experiment in experiments) + ) + + +class MicroArrayExpressAccessionAgent(AccessionAgentBase): + """ + MicroArray ArrayExpress accession gathering agent. The data is fetched from + the BioStudies database. See https://www.ebi.ac.uk/biostudies/help and + https://www.ebi.ac.uk/biostudies/arrayexpress/help#programmatic for more + information about the API endpoints. + """ + + def __init__(self, options) -> None: + super().__init__(options) + + self.data_chunk_size = 100 + self.data_url = "https://www.ebi.ac.uk/biostudies/api/v1/search" + self.ids = self.get_ids() + + def build_query(self) -> dict: + """Returns a query dict for getting array/organism specific accessions.""" + query_dict = { + "directsub": "true", + "page": 1, + "pageSize": self.data_chunk_size, + "release_date": f"[{self.since} TO {self.until}]", + "type": "study", + } + + if self.ids: + # TODO(ark): figure out better way of array filtering. + # Also make sure it's equivalent to the array filtering in this query + # https://github.com/AlexsLemonade/accession_retrieval/blob/master/experiment_accession_retrieval.R#L208 + query_dict.update({"content": ", ".join(self.ids)}) + elif self.keyword: + query_dict.update({"content": self.keyword}) + elif self.organism: + query_dict.update({"organism": f'"{self.organism}"'}) + + return query_dict + + def collect_data(self) -> Set[str]: + """Gets new accessions from EBI Biostudies API.""" + accessions = set() + + if self.ids: + message = ( + "Getting MicroArray ArrayExpress entries by " + f"ArrayExpress ID(s): {', '.join(self.ids)} for [{self.since} - {self.until}] " + "range." + ) + elif self.keyword: + message = ( + "Getting MicroArray ArrayExpress entries by " + f'"{self.keyword}" keyword for [{self.since} - {self.until}] range.' + ) + elif self.organism: + message = ( + "Getting MicroArray ArrayExpress entries by " + f'"{self.organism}" organism for [{self.since} - {self.until}] range.' + ) + else: + return accessions + + log.debug(message) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self) -> Set[str]: + """Retrieves accessions from API search endpoint.""" + + @retry(**self.retry_params) + def get_response(url, **kwargs): + """Gets response from an API endpoint.""" + return requests.get(url, **kwargs) + + accessions = set() + + is_done = False + params = self.build_query() + while not is_done: + range_start = (params["page"] - 1) * params["pageSize"] + 1 + range_end = (params["page"] - 1) * params["pageSize"] + self.data_chunk_size + log.debug(f"Processing entries {range_start} - {range_end}") + + response = get_response(self.data_url, params=params) + entries = response.json().get("hits") + if entries: + entries = ( + AccessionBacklogEntry.create_from_ma_ae_entry(entry) for entry in entries + ) + params["page"] += 1 + else: + is_done = True + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + # Quit after getting a sufficient amount of accessions. + if self.count and len(accessions) >= self.count: + is_done = True + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed ArrayExpress IDs.""" + ids = set() + + if self.options["ae_id"]: + ids.update(self.options["ae_id"]) + + if self.options["ae_ids_file"]: + with open(self.options["ae_ids_file"]) as ae_ids_file: + ids.update((ae_id.strip() for ae_id in ae_ids_file.readlines())) + + return sorted(ids) + + +class MicroArrayGEOAccessionAgent(AccessionAgentBase): + """ + MicroArray GEO accession gathering agent. The data is fetched from a local + SQLite GEO meta database. + """ + + def __init__(self, options) -> None: + super().__init__(options) + + self.db_path = "data/microarray/GEOmetadb.sqlite" + self.ids = self.get_ids() + + def build_query(self) -> str: + """Returns a query for getting GEO accessions from the local SQLite meta DB.""" + tables = [ + f"SELECT *", + "FROM gse_gpl", + "JOIN gpl ON gse_gpl.gpl=gpl.gpl", + "JOIN gse ON gse.gse=gse_gpl.gse", + "GROUP BY gse_gpl.gse", + ] + + conditions = [ + f"HAVING gse.submission_date >= '{self.since}'", + f"gse.submission_date <= '{self.until}'", + ] + + if self.ids: + gpl_ids = (f"'{gpl_id}'" for gpl_id in self.ids) + conditions.append(f"gse_gpl.gpl IN ({', '.join(gpl_ids)})") + elif self.organism: + conditions.append(f"lower(organism)='{self.organism.lower()}'") + + return f"{' '.join(tables)} {' AND '.join(conditions)}" + + def collect_data(self) -> Set[str]: + """Gets new accessions from GEO database.""" + accessions = set() + + if self.ids: + message = ( + "Getting MicroArray GEO entries by GEO platform ID(s): " + f"{', '.join(self.ids)} for [{self.since} - {self.until}] range." + ) + elif self.keyword: + message = ( + f'Getting MicroArray GEO entries by "{self.keyword}" keyword ' + f"for [{self.since} - {self.until}] range." + ) + elif self.organism: + message = ( + f'Getting MicroArray GEO entries by "{self.organism}" organism ' + f"for [{self.since} - {self.until}] range." + ) + else: + return accessions + + log.debug(message) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self) -> Set[str]: + """Retrieves accessions from the GEO meta DB.""" + + def match_keyword(row): + """ + Returns True if `row` matches `self.keyword` based regex. + Otherwise returns False. + """ + return re_keyword.match(" ".join((str(c) for c in row if c))) + + accessions = set() + + if not os.path.exists(self.db_path): + log.error("GEO meta database doesn't exist.") + return accessions + + connection = sqlite3.connect(self.db_path) + connection.row_factory = sqlite3.Row + connection.text_factory = lambda b: b.decode(errors="ignore") + entries = connection.execute(self.build_query()).fetchall() + connection.close() + + if self.keyword: + re_keyword = re.compile(f".*{self.keyword}.*", re.IGNORECASE) # Keyword regex. + entries = filter(match_keyword, entries) + + entries = ({key.lower(): entry[key] for key in entry.keys()} for entry in entries) + entries = set((AccessionBacklogEntry.create_from_ma_geo_entry(entry) for entry in entries)) + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed GEO platform IDs.""" + ids = set() + + if self.options["gpl_id"]: + ids.update(self.options["gpl_id"]) + + if self.options["gpl_ids_file"]: + with open(self.options["gpl_ids_file"]) as gpl_ids_file: + ids.update((gpl_id.strip() for gpl_id in gpl_ids_file.readlines())) + + return sorted(ids) + + +class RNASeqAccessionAgent(AccessionAgentBase): + """ + RNA-Seq accession gathering agent. The data is fetched from + The European Nucleotide Archive (ENA) Portal. + See https://www.ebi.ac.uk/ena/portal/api/ for more information about the API + endpoints. + """ + + def __init__(self, options) -> None: + super().__init__(options) + + self.data_chunk_size = 10000 + self.data_url = "https://www.ebi.ac.uk/ena/portal/api/search" + self.ids = self.get_ids() + + def build_query(self, taxon_id: str = None) -> str: + """ + Returns a query to use for getting specific taxon ID accessions. + Some special characters must remain unquoted. + """ + + AND = " AND " + OR = " OR " + instrument_models = ( + "HiSeq X Five", + "HiSeq X Ten", + "Illumina Genome Analyzer II", + "Illumina Genome Analyzer IIx", + "Illumina Genome Analyzer", + "Illumina HiScanSQ", + "Illumina HiSeq 1000", + "Illumina HiSeq 1500", + "Illumina HiSeq 2000", + "Illumina HiSeq 2500", + "Illumina HiSeq 3000", + "Illumina HiSeq 4000", + "Illumina MiSeq", + "Illumina NovaSeq 6000", + "Ion Torrent Proton", + "Ion Torrent S5 XL", + "Ion Torrent S5", + "NextSeq 500", + "NextSeq 550", + ) + + instrument_models = OR.join((f'instrument_model="{im}"' for im in instrument_models)) + conditions = [ + # Relevant date fields: collection_date, collection_date_submitted, + # first_public, last_updated. + f"first_public >= {self.since}", + f"first_public <= {self.until}", + f"({instrument_models})", + 'library_source="TRANSCRIPTOMIC"', + 'library_strategy="RNA-Seq"', + ] + + if taxon_id: + conditions.append(f"tax_eq({taxon_id})") + elif self.keyword: + search_fields = ( + "assembly_software", + "bio_material", + "center_name", + "collected_by", + "experiment_title", + "host_body_site", + "instrument_model", + "instrument_platform", + "library_name", + "project_name", + "sample_title", + "sequencing_method", + "study_title", + ) + search_fields = OR.join( + (f'{sf}="*{self.keyword}*"' for sf in search_fields) + ) # Keyword regex. + conditions.append(f"({search_fields})") + elif self.organism: + # `host`: Natural (as opposed to laboratory) host to the organism from which sample + # was obtained. + # `host_scientific_name`: Scientific name of the natural (as opposed to laboratory) + # host to the organism from which sample was obtained. + # `scientific_name` Scientific name of the organism from which the sample was derived. + # Neither `host_scientific_name` nor `scientific_name` available for search. + # https://www.ebi.ac.uk/ena/portal/api/searchFields?dataPortal=ena&format=json&result=read_study + conditions.append(f'host="{self.organism}"') + + return quote(AND.join(conditions), safe='*()-="<>/ ') # Must remain unquoted. + + def collect_data(self) -> Set[str]: + """Gets new accessions from EBI ENA API.""" + accessions = set() + + if self.ids: + log.debug( + f"Getting RNA-Seq entries by taxon ID(s): " + f"{', '.join((str(idx) for idx in self.ids))} for [{self.since} - {self.until}] range." + ) + total = len(self.ids) + for idx, taxon_id in enumerate(self.ids): + if self.count and len(accessions) >= self.count: + break + + if total > 1: + log.debug(f"Getting entries for taxon ID {taxon_id}, {idx + 1} of {total}.") + accessions.update(self.fetch_data(taxon_id=taxon_id)) + elif self.keyword: + log.debug( + f'Getting RNA-Seq entries by "{self.keyword}" keyword ' + f"for [{self.since} - {self.until}] range." + ) + accessions.update(self.fetch_data()) + elif self.organism: + log.debug( + f'Getting entries by "{self.organism}" organism ' + f"for [{self.since} - {self.until}] range." + ) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self, taxon_id=None) -> Set[str]: + """ + Retrieves accessions from API search endpoint. + The API allows to set limit to 0 (get all in one request) but we do + it in a paginated fashion with `self.data_chunk_size` as a page size. + """ + + @retry(**self.retry_params) + def get_response(url, **kwargs): + """Gets response from an API endpoint.""" + return requests.post(url, **kwargs) + + accessions = set() + + fields = [ + "first_public", + "scientific_name", + "secondary_study_accession", + ] # For DRP/ERP/SRP-prefixed accessions. + data = { + "dataPortal": "ena", + # TODO(ark): add excludeAccessions/excludeAccessionType support. + "fields": ",".join(fields), # Use "all" to get all fields. + "format": "json", + "limit": self.data_chunk_size, + "offset": 0, + "query": self.build_query(taxon_id=taxon_id), + "result": "read_study", + "sortFields": fields, + } + + is_done = False + while not is_done: + log.debug( + f"Processing entries {data['offset'] + 1} - {data['offset'] + self.data_chunk_size}" + ) + entries = () + try: + response = get_response(self.data_url, data=data) + entries = response.json() + # TODO(ark): add `organism` when -o, --organism flag is used. + entries = ( + AccessionBacklogEntry.create_from_rnaseq_entry(entry) for entry in entries + ) + except JSONDecodeError: + is_done = True + except TypeError: + log.error(f"Couldn't get data from {self.data_url}. Response: {entries}") + data["offset"] += self.data_chunk_size + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + # Quit after getting a sufficient amount of accessions. + if self.count and len(accessions) >= self.count: + is_done = True + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed taxon IDs.""" + ids = set() + + if self.options["taxon_id"]: + ids.update(self.options["taxon_id"]) + + if self.options["taxon_ids_file"]: + with open(self.options["taxon_ids_file"]) as taxon_id_file: + ids.update((taxon_id.strip() for taxon_id in taxon_id_file.readlines())) + + return sorted(ids) diff --git a/foreman/data_refinery_foreman/settings.py b/foreman/data_refinery_foreman/settings.py index 7a489facc..5fea76d71 100644 --- a/foreman/data_refinery_foreman/settings.py +++ b/foreman/data_refinery_foreman/settings.py @@ -47,6 +47,7 @@ "data_refinery_common", "data_refinery_foreman.surveyor", "data_refinery_foreman.foreman", + "data_refinery_foreman.gatherer", "raven.contrib.django.raven_compat", "computedfields", ] @@ -108,10 +109,18 @@ # https://docs.djangoproject.com/en/1.10/ref/settings/#auth-password-validators AUTH_PASSWORD_VALIDATORS = [ - {"NAME": "django.contrib.auth.password_validation.UserAttributeSimilarityValidator",}, - {"NAME": "django.contrib.auth.password_validation.MinimumLengthValidator",}, - {"NAME": "django.contrib.auth.password_validation.CommonPasswordValidator",}, - {"NAME": "django.contrib.auth.password_validation.NumericPasswordValidator",}, + { + "NAME": "django.contrib.auth.password_validation.UserAttributeSimilarityValidator", + }, + { + "NAME": "django.contrib.auth.password_validation.MinimumLengthValidator", + }, + { + "NAME": "django.contrib.auth.password_validation.CommonPasswordValidator", + }, + { + "NAME": "django.contrib.auth.password_validation.NumericPasswordValidator", + }, ] diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 8c09c6888..929ef2476 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -8,6 +8,8 @@ RUN apt-get -y install apt-fast RUN apt-fast update -qq && \ apt-fast install -y \ + gcc \ + libpq-dev \ python3 \ python3-pip From 47d13d431917c78ef2d42e548b00bbca54b4a2b6 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 9 Sep 2022 12:12:16 -0700 Subject: [PATCH 013/220] Add accession gathering cron job. --- config/rna_seq_taxon_ids.txt | 247 ++++++++++++++++++ .../commands/gather_weekly_accessions.py | 30 +++ .../foreman-server-instance-user-data.tpl.sh | 1 + 3 files changed, 278 insertions(+) create mode 100644 config/rna_seq_taxon_ids.txt create mode 100644 foreman/data_refinery_foreman/gatherer/management/commands/gather_weekly_accessions.py diff --git a/config/rna_seq_taxon_ids.txt b/config/rna_seq_taxon_ids.txt new file mode 100644 index 000000000..6d284d3cc --- /dev/null +++ b/config/rna_seq_taxon_ids.txt @@ -0,0 +1,247 @@ +2769 +3055 +3218 +3625 +3641 +3659 +3694 +3702 +3708 +3711 +3712 +3760 +3847 +3871 +3880 +3885 +3914 +3983 +4039 +4113 +4232 +4528 +4529 +4530 +4533 +4536 +4537 +4538 +4555 +4558 +4572 +4577 +4641 +4932 +6183 +6238 +6239 +6279 +6282 +6334 +6412 +6669 +6945 +7029 +7070 +7091 +7159 +7165 +7176 +7209 +7217 +7220 +7222 +7227 +7230 +7234 +7237 +7238 +7240 +7244 +7245 +7260 +7375 +7425 +7460 +7574 +7668 +7719 +7757 +7764 +7897 +7918 +7955 +7994 +8010 +8049 +8078 +8083 +8090 +8128 +8154 +8364 +8502 +8508 +8663 +8790 +8823 +8824 +8839 +8996 +9031 +9103 +9157 +9305 +9315 +9358 +9361 +9365 +9371 +9483 +9516 +9531 +9545 +9565 +9601 +9606 +9615 +9627 +9646 +9669 +9685 +9739 +9785 +9796 +9813 +9823 +9913 +9925 +9940 +9978 +9986 +10089 +10090 +10093 +10096 +10103 +10116 +10141 +10228 +12957 +13146 +13249 +13333 +13616 +13686 +13735 +15368 +27923 +28377 +28743 +29159 +29730 +29760 +30195 +30464 +30538 +30611 +30732 +31033 +31234 +32264 +32473 +32507 +33528 +34506 +34740 +34839 +35525 +36166 +37347 +37548 +37653 +37682 +38626 +39758 +40148 +40149 +40217 +42254 +43151 +43179 +44394 +45157 +45351 +48698 +48701 +48709 +49451 +51511 +52283 +52904 +54126 +55577 +57577 +59463 +59689 +59729 +59894 +60711 +61819 +61853 +64144 +65489 +69293 +72004 +72036 +74533 +77166 +77586 +79684 +80972 +81970 +83772 +88036 +93934 +94237 +96440 +99883 +106582 +106734 +113334 +113540 +121225 +126957 +130081 +132113 +132585 +132908 +135651 +136037 +139649 +144197 +157791 +158441 +161767 +161934 +179676 +198806 +205130 +210143 +217634 +225164 +230844 +242159 +281687 +283909 +299123 +303518 +315563 +321398 +328815 +336983 +400682 +407821 +409849 +452646 +669202 diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_weekly_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_weekly_accessions.py new file mode 100644 index 000000000..35b27a5ab --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_weekly_accessions.py @@ -0,0 +1,30 @@ +"""A wrapper for `gather_accessions` command which can be executed as a cron job.""" + +from datetime import datetime + +from django.core.management import call_command +from django.core.management.base import BaseCommand + +from dateutil.relativedelta import SU, relativedelta + + +class Command(BaseCommand): + """Gathers accessions published since previous Sunday.""" + + def handle(self, *args, **options): + """Runs accession gathering.""" + last_sunday = (datetime.now() - relativedelta(weekday=SU(-1))).strftime("%Y-%m-%d") + + # TODO(ark): make the command process all sources within a single run. + call_command( + "gather_accessions", + "--microarray", + since=last_sunday, + organism="homo sapiens", + ) + call_command( + "gather_accessions", + "--rna-seq", + since=last_sunday, + taxon_ids_file="config/rna_seq_taxon_ids.txt", + ) diff --git a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh index e77541c75..f14e9bcc1 100644 --- a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh +++ b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh @@ -114,6 +114,7 @@ crontab -l > tempcron cat <> tempcron 0 12 * * MON /bin/bash /home/ubuntu/run_cron_job_test.sh affymetrix check_brainarray_gene_agreement >> /var/log/cron_job_tests.log 2>&1 0 12 * * MON /bin/bash /home/ubuntu/run_cron_job_test.sh affymetrix check_tx_index_transcript_agreement >> /var/log/cron_job_tests.log 2>&1 +0 12 * * SUN /bin/bash /home/ubuntu/run_management_command.sh gather_weekly_accessions >> /var/log/gather_weekly_accessions.log 2>&1 EOF # install new cron file crontab tempcron From 9e520a0e0834368830fa73e409cd23c29de01587 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 13 Sep 2022 17:12:41 -0700 Subject: [PATCH 014/220] Address review comments. --- .../migrations/0071_auto_20220901_1653.py | 44 -- ...klogentry.py => 0071_gatheredaccession.py} | 6 +- .../data_refinery_common/models/__init__.py | 2 +- .../{accession.py => gathered_accession.py} | 20 +- .../gatherer/agents/__init__.py | 0 .../gatherer/agents/base.py | 79 +++ .../gatherer/agents/microarray_ae.py | 126 ++++ .../gatherer/agents/microarray_geo.py | 123 ++++ .../gatherer/agents/rna_seq.py | 204 ++++++ .../management/commands/gather_accessions.py | 643 +++--------------- 10 files changed, 626 insertions(+), 621 deletions(-) delete mode 100644 common/data_refinery_common/migrations/0071_auto_20220901_1653.py rename common/data_refinery_common/migrations/{0071_accessionbacklogentry.py => 0071_gatheredaccession.py} (88%) rename common/data_refinery_common/models/{accession.py => gathered_accession.py} (84%) create mode 100644 foreman/data_refinery_foreman/gatherer/agents/__init__.py create mode 100644 foreman/data_refinery_foreman/gatherer/agents/base.py create mode 100644 foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py create mode 100644 foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py create mode 100644 foreman/data_refinery_foreman/gatherer/agents/rna_seq.py diff --git a/common/data_refinery_common/migrations/0071_auto_20220901_1653.py b/common/data_refinery_common/migrations/0071_auto_20220901_1653.py deleted file mode 100644 index c7d3b0b63..000000000 --- a/common/data_refinery_common/migrations/0071_auto_20220901_1653.py +++ /dev/null @@ -1,44 +0,0 @@ -# Generated by Django 3.2.7 on 2022-09-01 16:53 - -from django.db import migrations, models - - -class Migration(migrations.Migration): - - dependencies = [ - ("data_refinery_common", "0070_auto_20211208_2118"), - ] - - operations = [ - migrations.CreateModel( - name="Accession", - fields=[ - ( - "id", - models.AutoField( - auto_created=True, - primary_key=True, - serialize=False, - verbose_name="ID", - ), - ), - ("code", models.TextField()), - ("created_at", models.DateTimeField(auto_now_add=True)), - ("last_modified_at", models.DateTimeField(auto_now=True)), - ("organism", models.TextField()), - ("published_date", models.DateTimeField()), - ("sample_count", models.PositiveIntegerField(default=0)), - ("source", models.TextField()), - ("technology", models.TextField()), - ], - options={ - "db_table": "accessions", - }, - ), - migrations.AddConstraint( - model_name="accession", - constraint=models.UniqueConstraint( - fields=("code", "source", "technology"), name="unique_accession" - ), - ), - ] diff --git a/common/data_refinery_common/migrations/0071_accessionbacklogentry.py b/common/data_refinery_common/migrations/0071_gatheredaccession.py similarity index 88% rename from common/data_refinery_common/migrations/0071_accessionbacklogentry.py rename to common/data_refinery_common/migrations/0071_gatheredaccession.py index 86c04daed..a1740d96e 100644 --- a/common/data_refinery_common/migrations/0071_accessionbacklogentry.py +++ b/common/data_refinery_common/migrations/0071_gatheredaccession.py @@ -1,4 +1,4 @@ -# Generated by Django 3.2.7 on 2022-09-07 19:31 +# Generated by Django 3.2.7 on 2022-09-13 18:14 from django.db import migrations, models @@ -11,7 +11,7 @@ class Migration(migrations.Migration): operations = [ migrations.CreateModel( - name="AccessionBacklogEntry", + name="GatheredAccession", fields=[ ( "id", @@ -32,7 +32,7 @@ class Migration(migrations.Migration): ("technology", models.TextField()), ], options={ - "db_table": "accession_backlog", + "db_table": "gathered_accessions", }, ), ] diff --git a/common/data_refinery_common/models/__init__.py b/common/data_refinery_common/models/__init__.py index 8e9564153..2b544765d 100644 --- a/common/data_refinery_common/models/__init__.py +++ b/common/data_refinery_common/models/__init__.py @@ -1,4 +1,3 @@ -from data_refinery_common.models.accession import Accession # noqa from data_refinery_common.models.api_token import APIToken # noqa from data_refinery_common.models.associations.compendium_result_organism_association import ( # noqa CompendiumResultOrganismAssociation, @@ -46,6 +45,7 @@ from data_refinery_common.models.dataset_annotation import DatasetAnnotation # noqa from data_refinery_common.models.experiment import Experiment # noqa from data_refinery_common.models.experiment_annotation import ExperimentAnnotation # noqa +from data_refinery_common.models.gathered_accession import GatheredAccession # noqa from data_refinery_common.models.jobs.downloader_job import DownloaderJob # noqa from data_refinery_common.models.jobs.processor_job import ProcessorJob # noqa from data_refinery_common.models.jobs.survey_job import SurveyJob # noqa diff --git a/common/data_refinery_common/models/accession.py b/common/data_refinery_common/models/gathered_accession.py similarity index 84% rename from common/data_refinery_common/models/accession.py rename to common/data_refinery_common/models/gathered_accession.py index 6ac62da9f..04b084533 100644 --- a/common/data_refinery_common/models/accession.py +++ b/common/data_refinery_common/models/gathered_accession.py @@ -4,11 +4,11 @@ from django.utils import timezone -class AccessionBacklogEntry(models.Model): - """Accession backlog entry model.""" +class GatheredAccession(models.Model): + """Gathered accession model.""" class Meta: - db_table = "accession_backlog" + db_table = "gathered_accessions" code = models.TextField(unique=True) created_at = models.DateTimeField(auto_now_add=True) @@ -21,7 +21,7 @@ class Meta: def __eq__(self, other: object) -> bool: """Returns True if two objects are equal. Otherwise returns False.""" - return isinstance(other, AccessionBacklogEntry) and self.code == other.code + return isinstance(other, GatheredAccession) and self.code == other.code def __hash__(self) -> int: """Returns accession object unique hash value.""" @@ -32,15 +32,15 @@ def __str__(self) -> str: return ", ".join((self.code, self.technology, self.source, str(self.published_date.date()))) @staticmethod - def create_from_ma_ae_entry(entry): + def create_from_ma_ae_entry(entry, organism=None): """Creates accession object from MicroArray ArrayExpress entry.""" - accession = AccessionBacklogEntry() + accession = GatheredAccession() accession.code = entry["accession"] accession.source = "ebi_biostudies" accession.technology = "microarray" - if "organism" in entry: - accession.organism = entry["organism"] + if organism: + accession.organism = organism if "release_date" in entry: accession.published_date = timezone.make_aware( datetime.strptime(entry["release_date"], "%Y-%m-%d") @@ -51,7 +51,7 @@ def create_from_ma_ae_entry(entry): @staticmethod def create_from_ma_geo_entry(entry): """Creates accession object from MicroArray GEO meta DB entry.""" - accession = AccessionBacklogEntry() + accession = GatheredAccession() accession.code = entry["gse"] accession.source = "geo_meta_db" accession.technology = "microarray" @@ -69,7 +69,7 @@ def create_from_ma_geo_entry(entry): @staticmethod def create_from_rnaseq_entry(entry): """Creates accession object from RNA-Seq entry.""" - accession = AccessionBacklogEntry() + accession = GatheredAccession() accession.code = entry["secondary_study_accession"] accession.source = "ebi_ena_portal" accession.technology = "rna-seq" diff --git a/foreman/data_refinery_foreman/gatherer/agents/__init__.py b/foreman/data_refinery_foreman/gatherer/agents/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/gatherer/agents/base.py b/foreman/data_refinery_foreman/gatherer/agents/base.py new file mode 100644 index 000000000..3754a4068 --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/agents/base.py @@ -0,0 +1,79 @@ +"""Abstract base class for accession gathering automation agents.""" + +from abc import ABC, abstractmethod +from datetime import datetime +from http.client import RemoteDisconnected + +from requests.exceptions import ConnectionError, ConnectTimeout +from urllib3.exceptions import ProtocolError + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.experiment import Experiment +from data_refinery_common.models.gathered_accession import GatheredAccession + +logger = get_and_configure_logger(__name__) + + +class AccessionAgentBase(ABC): + "Accession agent abstract base class." + + previous_accessions = set() + retry_params = { + "retry_on_exception": lambda e: isinstance( + e, (ConnectionError, ConnectTimeout, ProtocolError, RemoteDisconnected) + ), + "stop_max_attempt_number": 5, + "wait_exponential_multiplier": 1000, # Seconds. + "wait_exponential_max": 16000, # Seconds. + } + + def __init__(self, options) -> None: + """Populates args and values for major variables.""" + self.options = options + self.count = options["count"] + self.keyword = options["keyword"] + self.organism = options["organism"] + self.since = options["since"] + self.until = options["until"] or datetime.now().strftime("%Y-%m-%d") + + self.ids = self.get_ids() + self.populate_previous_accessions() + + @abstractmethod + def build_query(self): + """Returns query/query dict depending on the accession data source.""" + pass + + @abstractmethod + def collect_data(self): + """Generates resulting entry collection.""" + pass + + @abstractmethod + def fetch_data(self): + """Fetches data from an external or local data source.""" + pass + + @abstractmethod + def get_ids(self): + """Gets IDs for query filtering depending on the accession technology.""" + pass + + def populate_previous_accessions(self) -> None: + """Populates previous accession set from a provided excluded ids file.""" + if not self.options["exclude_previous"] or self.previous_accessions: + return + + # Gathered accessions. + self.previous_accessions.update( + (entry["code"] for entry in GatheredAccession.objects.values("code")) + ) + + # Surveyed accessions. + experiments = Experiment.objects.values("accession_code", "alternate_accession_code") + self.previous_accessions.update( + (experiment["accession_code"] for experiment in experiments) + ) + self.previous_accessions.update( + (experiment["alternate_accession_code"] for experiment in experiments) + ) diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py new file mode 100644 index 000000000..b5314302b --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py @@ -0,0 +1,126 @@ +"""MicroArray ArrayExpress accession gathering automation. +Data source: https://www.ebi.ac.uk/biostudies/help""" + +from typing import List, Set + +import requests +from retrying import retry + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.gathered_accession import GatheredAccession +from data_refinery_foreman.gatherer.agents.base import AccessionAgentBase + +logger = get_and_configure_logger(__name__) + + +class MicroArrayExpressAccessionAgent(AccessionAgentBase): + """ + MicroArray ArrayExpress accession gathering agent. The data is fetched from + the BioStudies database. See https://www.ebi.ac.uk/biostudies/help and + https://www.ebi.ac.uk/biostudies/arrayexpress/help#programmatic for more + information about the API endpoints. + """ + + DATA_CHUNK_SIZE = 100 + DATA_URL = "https://www.ebi.ac.uk/biostudies/api/v1/search" + + def build_query(self) -> dict: + """Returns a query dict for getting array/organism specific accessions.""" + query_dict = { + "directsub": "true", + "page": 1, + "pageSize": self.DATA_CHUNK_SIZE, + "release_date": f"[{self.since} TO {self.until}]", + "type": "study", + } + + if self.ids: + # TODO(ark): figure out better way of array filtering. + # Also make sure it's equivalent to the array filtering in this query + # https://github.com/AlexsLemonade/accession_retrieval/blob/master/experiment_accession_retrieval.R#L208 + query_dict.update({"content": ", ".join(self.ids)}) + elif self.keyword: + query_dict.update({"content": self.keyword}) + elif self.organism: + query_dict.update({"organism": f'"{self.organism}"'}) + + return query_dict + + def collect_data(self) -> Set[str]: + """Gets new accessions from EBI Biostudies API.""" + accessions = set() + + if self.ids: + message = ( + "Getting MicroArray ArrayExpress entries by " + f"ArrayExpress ID(s): {', '.join(self.ids)} for [{self.since} - {self.until}] " + "range." + ) + elif self.keyword: + message = ( + "Getting MicroArray ArrayExpress entries by " + f'"{self.keyword}" keyword for [{self.since} - {self.until}] range.' + ) + elif self.organism: + message = ( + "Getting MicroArray ArrayExpress entries by " + f'"{self.organism}" organism for [{self.since} - {self.until}] range.' + ) + else: + return accessions + + logger.debug(message) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self) -> Set[str]: + """Retrieves accessions from API search endpoint.""" + + @retry(**self.retry_params) + def get_response(url, **kwargs): + """Gets response from an API endpoint.""" + return requests.get(url, **kwargs) + + accessions = set() + + is_done = False + params = self.build_query() + while not is_done: + range_start = (params["page"] - 1) * params["pageSize"] + 1 + range_end = (params["page"] - 1) * params["pageSize"] + self.DATA_CHUNK_SIZE + logger.debug(f"Processing entries {range_start} - {range_end}") + + response = get_response(self.DATA_URL, params=params) + entries = response.json().get("hits") + if entries: + entries = ( + GatheredAccession.create_from_ma_ae_entry(entry, organism=self.organism) + for entry in entries + ) + params["page"] += 1 + else: + is_done = True + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + # Quit after getting a sufficient amount of accessions. + if self.count and len(accessions) >= self.count: + is_done = True + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed ArrayExpress IDs.""" + ids = set() + + if self.options["ae_id"]: + ids.update(self.options["ae_id"]) + + if self.options["ae_ids_file"]: + with open(self.options["ae_ids_file"]) as ae_ids_file: + ids.update((ae_id.strip() for ae_id in ae_ids_file.readlines())) + + return sorted(ids) diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py new file mode 100644 index 000000000..975c715b3 --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py @@ -0,0 +1,123 @@ +"""MicroArray GEO accession gathering automation. +Data source: local SQLite meta DB from https://www.bioconductor.org/packages/release/bioc/html/GEOmetadb.html""" + +import os +import re +import sqlite3 +from typing import List, Set + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.gathered_accession import GatheredAccession +from data_refinery_foreman.gatherer.agents.base import AccessionAgentBase + +logger = get_and_configure_logger(__name__) + + +class MicroArrayGEOAccessionAgent(AccessionAgentBase): + """ + MicroArray GEO accession gathering agent. The data is fetched from a local + SQLite GEO meta database. + """ + + # TODO(ark): move the DB file from Docker image to S3. + # Implement syncing procedure. + # Update URL once the original file is available again. + DB_PATH = "data/microarray/GEOmetadb.sqlite" + + def build_query(self) -> str: + """Returns a query for getting GEO accessions from the local SQLite meta DB.""" + tables = [ + "SELECT *", + "FROM gse_gpl", + "JOIN gpl ON gse_gpl.gpl=gpl.gpl", + "JOIN gse ON gse.gse=gse_gpl.gse", + "GROUP BY gse_gpl.gse", + ] + + conditions = [ + f"HAVING gse.submission_date >= '{self.since}'", + f"gse.submission_date <= '{self.until}'", + ] + + if self.ids: + gpl_ids = (f"'{gpl_id}'" for gpl_id in self.ids) + conditions.append(f"gse_gpl.gpl IN ({', '.join(gpl_ids)})") + elif self.organism: + conditions.append(f"lower(organism)='{self.organism.lower()}'") + + return f"{' '.join(tables)} {' AND '.join(conditions)}" + + def collect_data(self) -> Set[str]: + """Gets new accessions from GEO database.""" + accessions = set() + + if self.ids: + message = ( + "Getting MicroArray GEO entries by GEO platform ID(s): " + f"{', '.join(self.ids)} for [{self.since} - {self.until}] range." + ) + elif self.keyword: + message = ( + f'Getting MicroArray GEO entries by "{self.keyword}" keyword ' + f"for [{self.since} - {self.until}] range." + ) + elif self.organism: + message = ( + f'Getting MicroArray GEO entries by "{self.organism}" organism ' + f"for [{self.since} - {self.until}] range." + ) + else: + return accessions + + logger.debug(message) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self) -> Set[str]: + """Retrieves accessions from the GEO meta DB.""" + + def match_keyword(row): + """ + Returns True if `row` matches `self.keyword` based regex. + Otherwise returns False. + """ + return re_keyword.match(" ".join((str(c) for c in row if c))) + + accessions = set() + + if not os.path.exists(self.DB_PATH): + logger.error("GEO meta database doesn't exist.") + return accessions + + connection = sqlite3.connect(self.DB_PATH) + connection.row_factory = sqlite3.Row + connection.text_factory = lambda b: b.decode(errors="ignore") + entries = connection.execute(self.build_query()).fetchall() + connection.close() + + if self.keyword: + re_keyword = re.compile(f".*{self.keyword}.*", re.IGNORECASE) # Keyword regex. + entries = filter(match_keyword, entries) + + entries = ({key.lower(): entry[key] for key in entry.keys()} for entry in entries) + entries = set((GatheredAccession.create_from_ma_geo_entry(entry) for entry in entries)) + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed GEO platform IDs.""" + ids = set() + + if self.options["gpl_id"]: + ids.update(self.options["gpl_id"]) + + if self.options["gpl_ids_file"]: + with open(self.options["gpl_ids_file"]) as gpl_ids_file: + ids.update((gpl_id.strip() for gpl_id in gpl_ids_file.readlines())) + + return sorted(ids) diff --git a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py new file mode 100644 index 000000000..f9497f3ba --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py @@ -0,0 +1,204 @@ +"""RNA-Seq accession gathering automation. +Data source: https://www.ebi.ac.uk/ena/portal/api/""" + +from json.decoder import JSONDecodeError +from typing import List, Set +from urllib.parse import quote + +import requests +from retrying import retry + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.gathered_accession import GatheredAccession +from data_refinery_foreman.gatherer.agents.base import AccessionAgentBase + +logger = get_and_configure_logger(__name__) + + +class RNASeqAccessionAgent(AccessionAgentBase): + """ + RNA-Seq accession gathering agent. The data is fetched from + The European Nucleotide Archive (ENA) Portal. + See https://www.ebi.ac.uk/ena/portal/api/ for more information about the API + endpoints. + """ + + DATA_CHUNK_SIZE = 10000 + DATA_URL = "https://www.ebi.ac.uk/ena/portal/api/search" + + def build_query(self, taxon_id: str = None) -> str: + """ + Returns a query to use for getting specific taxon ID accessions. + Some special characters must remain unquoted. + """ + + AND = " AND " + OR = " OR " + instrument_models = ( + "HiSeq X Five", + "HiSeq X Ten", + "Illumina Genome Analyzer II", + "Illumina Genome Analyzer IIx", + "Illumina Genome Analyzer", + "Illumina HiScanSQ", + "Illumina HiSeq 1000", + "Illumina HiSeq 1500", + "Illumina HiSeq 2000", + "Illumina HiSeq 2500", + "Illumina HiSeq 3000", + "Illumina HiSeq 4000", + "Illumina MiSeq", + "Illumina NovaSeq 6000", + "Ion Torrent Proton", + "Ion Torrent S5 XL", + "Ion Torrent S5", + "NextSeq 500", + "NextSeq 550", + ) + + instrument_models = OR.join((f'instrument_model="{im}"' for im in instrument_models)) + conditions = [ + # Relevant date fields: collection_date, collection_date_submitted, + # first_public, last_updated. + f"first_public >= {self.since}", + f"first_public <= {self.until}", + f"({instrument_models})", + 'library_source="TRANSCRIPTOMIC"', + 'library_strategy="RNA-Seq"', + ] + + if taxon_id: + conditions.append(f"tax_eq({taxon_id})") + elif self.keyword: + search_fields = ( + "assembly_software", + "bio_material", + "center_name", + "collected_by", + "experiment_title", + "host_body_site", + "instrument_model", + "instrument_platform", + "library_name", + "project_name", + "sample_title", + "sequencing_method", + "study_title", + ) + search_fields = OR.join( + (f'{sf}="*{self.keyword}*"' for sf in search_fields) + ) # Keyword regex. + conditions.append(f"({search_fields})") + elif self.organism: + # `host`: Natural (as opposed to laboratory) host to the organism from which sample + # was obtained. + # `host_scientific_name`: Scientific name of the natural (as opposed to laboratory) + # host to the organism from which sample was obtained. + # `scientific_name` Scientific name of the organism from which the sample was derived. + # Neither `host_scientific_name` nor `scientific_name` available for search. + # https://www.ebi.ac.uk/ena/portal/api/searchFields?dataPortal=ena&format=json&result=read_study + conditions.append(f'host="{self.organism}"') + + return quote(AND.join(conditions), safe='*()-="<>/ ') # Must remain unquoted. + + def collect_data(self) -> Set[str]: + """Gets new accessions from EBI ENA API.""" + accessions = set() + + if self.ids: + logger.debug( + f"Getting RNA-Seq entries by taxon ID(s): " + f"{', '.join((str(i) for i in self.ids))} for [{self.since} - {self.until}] range." + ) + total = len(self.ids) + for idx, taxon_id in enumerate(self.ids): + if self.count and len(accessions) >= self.count: + break + + if total > 1: + logger.debug(f"Getting entries for taxon ID {taxon_id}, {idx + 1} of {total}.") + accessions.update(self.fetch_data(taxon_id=taxon_id)) + elif self.keyword: + logger.debug( + f'Getting RNA-Seq entries by "{self.keyword}" keyword ' + f"for [{self.since} - {self.until}] range." + ) + accessions.update(self.fetch_data()) + elif self.organism: + logger.debug( + f'Getting entries by "{self.organism}" organism ' + f"for [{self.since} - {self.until}] range." + ) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self, taxon_id=None) -> Set[str]: + """ + Retrieves accessions from API search endpoint. + The API allows to set limit to 0 (get all in one request) but we do + it in a paginated fashion with `self.DATA_CHUNK_SIZE` as a page size. + """ + + @retry(**self.retry_params) + def get_response(url, **kwargs): + """Gets response from an API endpoint.""" + return requests.post(url, **kwargs) + + accessions = set() + + fields = [ + "first_public", + "scientific_name", + "secondary_study_accession", + ] # For DRP/ERP/SRP-prefixed accessions. + data = { + "dataPortal": "ena", + # TODO(ark): add excludeAccessions/excludeAccessionType support. + "fields": ",".join(fields), # Use "all" to get all fields. + "format": "json", + "limit": self.DATA_CHUNK_SIZE, + "offset": 0, + "query": self.build_query(taxon_id=taxon_id), + "result": "read_study", + "sortFields": fields, + } + + is_done = False + while not is_done: + logger.debug( + f"Processing entries {data['offset'] + 1} - {data['offset'] + self.DATA_CHUNK_SIZE}" + ) + entries = () + try: + response = get_response(self.DATA_URL, data=data) + entries = response.json() + entries = (GatheredAccession.create_from_rnaseq_entry(entry) for entry in entries) + except JSONDecodeError: + is_done = True + except TypeError: + logger.error(f"Couldn't get data from {self.data_url}. Response: {entries}") + data["offset"] += self.DATA_CHUNK_SIZE + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + # Quit after getting a sufficient amount of accessions. + if self.count and len(accessions) >= self.count: + is_done = True + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed taxon IDs.""" + ids = set() + + if self.options["taxon_id"]: + ids.update(self.options["taxon_id"]) + + if self.options["taxon_ids_file"]: + with open(self.options["taxon_ids_file"]) as taxon_id_file: + ids.update((taxon_id.strip() for taxon_id in taxon_id_file.readlines())) + + return sorted(ids) diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index c4808a191..445245d3a 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -8,32 +8,27 @@ import argparse import logging -import os import re -import sqlite3 -from datetime import datetime -from http.client import RemoteDisconnected -from json.decoder import JSONDecodeError -from typing import List, Set -from urllib.parse import quote from django.core.management.base import BaseCommand -import requests -from requests.exceptions import ConnectionError, ConnectTimeout -from retrying import retry -from urllib3.exceptions import ProtocolError - from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models.accession import AccessionBacklogEntry -from data_refinery_common.models.experiment import Experiment +from data_refinery_common.models.gathered_accession import GatheredAccession +from data_refinery_foreman.gatherer.agents.microarray_ae import MicroArrayExpressAccessionAgent +from data_refinery_foreman.gatherer.agents.microarray_geo import MicroArrayGEOAccessionAgent +from data_refinery_foreman.gatherer.agents.rna_seq import RNASeqAccessionAgent -log = get_and_configure_logger(__name__) +logger = get_and_configure_logger(__name__) class Command(BaseCommand): """Creates agents and runs actual accession gathering.""" + DATA_SOURCE_MA_AE = "microarray-ae" + DATA_SOURCE_MA_GEO = "microarray-geo" + DATA_SOURCE_RNA_SEQ = "rna-seq" + DATA_SOURCES = (DATA_SOURCE_MA_AE, DATA_SOURCE_MA_GEO, DATA_SOURCE_RNA_SEQ) + RE_ACCESSION = re.compile(r"(\D+)(\d+)") RE_DATE = re.compile(r"\d{4}-\d{2}-\d{2}") @@ -76,14 +71,6 @@ def add_arguments(self, parser) -> None: default=True, help="Exclude previously gathered or surveyed accessions.", ) - parser.add_argument( - "-ne", - "--no-exclude-previous", - action="store_false", - default=False, - dest="exclude_previous", - help="Do not exclude previously gathered or surveyed accessions.", - ) parser.add_argument( "--gpl-id", action="extend", @@ -103,21 +90,22 @@ def add_arguments(self, parser) -> None: help="Keyword to use for filtering.", ) parser.add_argument( - "-m", - "--microarray", + "-lv", + "--log-verbose", action="store_true", default=False, - help="Collect MicroArray accessions.", + help="Enable verbose log output.", ) parser.add_argument( - "-o", "--organism", type=str, help="Organism name to use for filtering." + "-ne", + "--no-exclude-previous", + action="store_false", + default=False, + dest="exclude_previous", + help="Do not exclude previously gathered or surveyed accessions.", ) parser.add_argument( - "-r", - "--rna-seq", - action="store_true", - default=False, - help="Collect RNA-Seq accessions.", + "-o", "--organism", type=str, help="Organism name to use for filtering." ) parser.add_argument( "-s", @@ -126,6 +114,14 @@ def add_arguments(self, parser) -> None: required=True, help="Collect accessions made public on or after this date.", ) + parser.add_argument( + "-src", + "--source", + type=str, + action="extend", + nargs="+", + help="Gather accessions from selected sources.", + ) parser.add_argument( "--taxon-id", action="extend", @@ -144,28 +140,19 @@ def add_arguments(self, parser) -> None: type=str, help="Collect accessions made public before or on this date.", ) - parser.add_argument( - "-lv", - "--log-verbose", - action="store_true", - default=False, - help="Enable verbose log output.", - ) def set_verbosity_level(self, options) -> None: """Configures log verbosity level.""" if options["log_verbose"]: - log.addHandler(logging.StreamHandler()) - log.setLevel(logging.DEBUG) + logger.addHandler(logging.StreamHandler()) + logger.setLevel(logging.DEBUG) else: - log.setLevel(logging.ERROR) + logger.setLevel(logging.ERROR) def validate_args(self, options) -> None: """Validates arguments.""" - if not options["microarray"] and not options["rna_seq"]: - exit("Either --microarray or --rna-seq must be specified.") - errors = list() + since = options["since"] until = options["until"] if not self.RE_DATE.match(since): @@ -177,52 +164,65 @@ def validate_args(self, options) -> None: keyword = options["keyword"] organism = options["organism"] - if options["microarray"]: - ae_id = options["ae_id"] or options["ae_ids_file"] - gpl_id = options["gpl_id"] or options["gpl_ids_file"] - ids = ae_id or gpl_id - invalid_options_message = ( - "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " - "ArrayExpress ID(s) [--ae-id, --ae-ids-file] / GEO platform ID(s) " - "[--gpl-id, --gpl-ids-file] must be specified." - ) - elif options["rna_seq"]: - taxon_id = options["taxon_id"] or options["taxon_ids_file"] - ids = taxon_id - invalid_options_message = ( - "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] " - "or taxon ID(s) [--taxon-id, --taxon-ids-file] must be specified." + sources = options["source"] or self.DATA_SOURCES + + for source in sources: + if source in self.DATA_SOURCES: + continue + errors.append( + f"Unknown source: {source}. Supported sources: {', '.join(self.DATA_SOURCES)}" ) - if len([option for option in (ids, keyword, organism) if option]) != 1: - errors.append(invalid_options_message) + if self.DATA_SOURCE_MA_AE in sources: + ids = options["ae_id"] or options["ae_ids_file"] + if not (ids or keyword or organism): + errors.append( + ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " + "ArrayExpress ID(s) [--ae-id, --ae-ids-file] must be specified for " + f"'{self.DATA_SOURCE_MA_AE}' source." + ) + ) + if self.DATA_SOURCE_MA_GEO in sources: + ids = options["gpl_id"] or options["gpl_ids_file"] + if not (ids or keyword or organism): + errors.append( + ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " + "GEO platform ID(s) [--gpl-id, --gpl-ids-file] must be specified for " + f"'{self.DATA_SOURCE_MA_GEO}' source." + ) + ) + if self.DATA_SOURCE_RNA_SEQ in sources: + ids = options["taxon_id"] or options["taxon_ids_file"] + if not (ids or keyword or organism): + errors.append( + ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] " + "or taxon ID(s) [--taxon-id, --taxon-ids-file] must be specified for " + f"'{self.DATA_SOURCE_RNA_SEQ}' source." + ) + ) if errors: exit("\n".join(errors)) def handle(self, *args, **options): - """Runs the accession gathering process.""" + """Creates agents and runs the accession gathering process.""" self.validate_args(options) self.set_verbosity_level(options) agents = list() - if options["rna_seq"]: + sources = options["source"] or self.DATA_SOURCES + + if self.DATA_SOURCE_RNA_SEQ in sources: agents.append(RNASeqAccessionAgent(options)) - elif options["microarray"]: - if ( - options["ae_id"] - or options["ae_ids_file"] - or options["keyword"] - or options["organism"] - ): - agents.append(MicroArrayExpressAccessionAgent(options)) - if ( - options["gpl_id"] - or options["gpl_ids_file"] - or options["keyword"] - or options["organism"] - ): - agents.append(MicroArrayGEOAccessionAgent(options)) + + if self.DATA_SOURCE_MA_AE in sources: + agents.append(MicroArrayExpressAccessionAgent(options)) + + if self.DATA_SOURCE_MA_GEO in sources: + agents.append(MicroArrayGEOAccessionAgent(options)) entries = set() for agent in agents: @@ -245,487 +245,4 @@ def handle(self, *args, **options): output = "No accessions found." print(output) else: - AccessionBacklogEntry.objects.bulk_create(entries) - - -class AccessionAgentBase: - "Accession agent base class." - - previous_accessions = set() - retry_params = { - "retry_on_exception": lambda e: isinstance( - e, (ConnectionError, ConnectTimeout, ProtocolError, RemoteDisconnected) - ), - "stop_max_attempt_number": 5, - "wait_exponential_multiplier": 1000, # Seconds. - "wait_exponential_max": 16000, # Seconds. - } - - def __init__(self, options) -> None: - """Populates args and values for major variables.""" - self.options = options - self.count = options["count"] - self.keyword = options["keyword"] - self.organism = options["organism"] - self.since = options["since"] - self.until = options["until"] or datetime.now().strftime("%Y-%m-%d") - - self.populate_previous_accessions() - - def build_query(self): - """Returns query/query dict depending on the accession data source.""" - raise NotImplementedError - - def collect_data(self): - """Generates resulting entry collection.""" - raise NotImplementedError - - def fetch_data(self): - """Fetches data from an external or local data source.""" - raise NotImplementedError - - def get_ids(self): - """Gets IDs for query filtering depending on the accession technology.""" - raise NotImplementedError - - def populate_previous_accessions(self) -> None: - """Populates previous accession set from a provided excluded ids file.""" - if not self.options["exclude_previous"] or self.previous_accessions: - return - - # Gathered accessions. - self.previous_accessions.update( - (entry["code"] for entry in AccessionBacklogEntry.objects.values("code")) - ) - - # Surveyed accessions. - experiments = Experiment.objects.values("accession_code", "alternate_accession_code") - self.previous_accessions.update( - (experiment["accession_code"] for experiment in experiments) - ) - self.previous_accessions.update( - (experiment["alternate_accession_code"] for experiment in experiments) - ) - - -class MicroArrayExpressAccessionAgent(AccessionAgentBase): - """ - MicroArray ArrayExpress accession gathering agent. The data is fetched from - the BioStudies database. See https://www.ebi.ac.uk/biostudies/help and - https://www.ebi.ac.uk/biostudies/arrayexpress/help#programmatic for more - information about the API endpoints. - """ - - def __init__(self, options) -> None: - super().__init__(options) - - self.data_chunk_size = 100 - self.data_url = "https://www.ebi.ac.uk/biostudies/api/v1/search" - self.ids = self.get_ids() - - def build_query(self) -> dict: - """Returns a query dict for getting array/organism specific accessions.""" - query_dict = { - "directsub": "true", - "page": 1, - "pageSize": self.data_chunk_size, - "release_date": f"[{self.since} TO {self.until}]", - "type": "study", - } - - if self.ids: - # TODO(ark): figure out better way of array filtering. - # Also make sure it's equivalent to the array filtering in this query - # https://github.com/AlexsLemonade/accession_retrieval/blob/master/experiment_accession_retrieval.R#L208 - query_dict.update({"content": ", ".join(self.ids)}) - elif self.keyword: - query_dict.update({"content": self.keyword}) - elif self.organism: - query_dict.update({"organism": f'"{self.organism}"'}) - - return query_dict - - def collect_data(self) -> Set[str]: - """Gets new accessions from EBI Biostudies API.""" - accessions = set() - - if self.ids: - message = ( - "Getting MicroArray ArrayExpress entries by " - f"ArrayExpress ID(s): {', '.join(self.ids)} for [{self.since} - {self.until}] " - "range." - ) - elif self.keyword: - message = ( - "Getting MicroArray ArrayExpress entries by " - f'"{self.keyword}" keyword for [{self.since} - {self.until}] range.' - ) - elif self.organism: - message = ( - "Getting MicroArray ArrayExpress entries by " - f'"{self.organism}" organism for [{self.since} - {self.until}] range.' - ) - else: - return accessions - - log.debug(message) - accessions.update(self.fetch_data()) - - return accessions - - def fetch_data(self) -> Set[str]: - """Retrieves accessions from API search endpoint.""" - - @retry(**self.retry_params) - def get_response(url, **kwargs): - """Gets response from an API endpoint.""" - return requests.get(url, **kwargs) - - accessions = set() - - is_done = False - params = self.build_query() - while not is_done: - range_start = (params["page"] - 1) * params["pageSize"] + 1 - range_end = (params["page"] - 1) * params["pageSize"] + self.data_chunk_size - log.debug(f"Processing entries {range_start} - {range_end}") - - response = get_response(self.data_url, params=params) - entries = response.json().get("hits") - if entries: - entries = ( - AccessionBacklogEntry.create_from_ma_ae_entry(entry) for entry in entries - ) - params["page"] += 1 - else: - is_done = True - - if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) - accessions.update(entries) - - # Quit after getting a sufficient amount of accessions. - if self.count and len(accessions) >= self.count: - is_done = True - - return accessions - - def get_ids(self) -> List[str]: - """Returns a combined list of passed ArrayExpress IDs.""" - ids = set() - - if self.options["ae_id"]: - ids.update(self.options["ae_id"]) - - if self.options["ae_ids_file"]: - with open(self.options["ae_ids_file"]) as ae_ids_file: - ids.update((ae_id.strip() for ae_id in ae_ids_file.readlines())) - - return sorted(ids) - - -class MicroArrayGEOAccessionAgent(AccessionAgentBase): - """ - MicroArray GEO accession gathering agent. The data is fetched from a local - SQLite GEO meta database. - """ - - def __init__(self, options) -> None: - super().__init__(options) - - self.db_path = "data/microarray/GEOmetadb.sqlite" - self.ids = self.get_ids() - - def build_query(self) -> str: - """Returns a query for getting GEO accessions from the local SQLite meta DB.""" - tables = [ - f"SELECT *", - "FROM gse_gpl", - "JOIN gpl ON gse_gpl.gpl=gpl.gpl", - "JOIN gse ON gse.gse=gse_gpl.gse", - "GROUP BY gse_gpl.gse", - ] - - conditions = [ - f"HAVING gse.submission_date >= '{self.since}'", - f"gse.submission_date <= '{self.until}'", - ] - - if self.ids: - gpl_ids = (f"'{gpl_id}'" for gpl_id in self.ids) - conditions.append(f"gse_gpl.gpl IN ({', '.join(gpl_ids)})") - elif self.organism: - conditions.append(f"lower(organism)='{self.organism.lower()}'") - - return f"{' '.join(tables)} {' AND '.join(conditions)}" - - def collect_data(self) -> Set[str]: - """Gets new accessions from GEO database.""" - accessions = set() - - if self.ids: - message = ( - "Getting MicroArray GEO entries by GEO platform ID(s): " - f"{', '.join(self.ids)} for [{self.since} - {self.until}] range." - ) - elif self.keyword: - message = ( - f'Getting MicroArray GEO entries by "{self.keyword}" keyword ' - f"for [{self.since} - {self.until}] range." - ) - elif self.organism: - message = ( - f'Getting MicroArray GEO entries by "{self.organism}" organism ' - f"for [{self.since} - {self.until}] range." - ) - else: - return accessions - - log.debug(message) - accessions.update(self.fetch_data()) - - return accessions - - def fetch_data(self) -> Set[str]: - """Retrieves accessions from the GEO meta DB.""" - - def match_keyword(row): - """ - Returns True if `row` matches `self.keyword` based regex. - Otherwise returns False. - """ - return re_keyword.match(" ".join((str(c) for c in row if c))) - - accessions = set() - - if not os.path.exists(self.db_path): - log.error("GEO meta database doesn't exist.") - return accessions - - connection = sqlite3.connect(self.db_path) - connection.row_factory = sqlite3.Row - connection.text_factory = lambda b: b.decode(errors="ignore") - entries = connection.execute(self.build_query()).fetchall() - connection.close() - - if self.keyword: - re_keyword = re.compile(f".*{self.keyword}.*", re.IGNORECASE) # Keyword regex. - entries = filter(match_keyword, entries) - - entries = ({key.lower(): entry[key] for key in entry.keys()} for entry in entries) - entries = set((AccessionBacklogEntry.create_from_ma_geo_entry(entry) for entry in entries)) - - if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) - accessions.update(entries) - - return accessions - - def get_ids(self) -> List[str]: - """Returns a combined list of passed GEO platform IDs.""" - ids = set() - - if self.options["gpl_id"]: - ids.update(self.options["gpl_id"]) - - if self.options["gpl_ids_file"]: - with open(self.options["gpl_ids_file"]) as gpl_ids_file: - ids.update((gpl_id.strip() for gpl_id in gpl_ids_file.readlines())) - - return sorted(ids) - - -class RNASeqAccessionAgent(AccessionAgentBase): - """ - RNA-Seq accession gathering agent. The data is fetched from - The European Nucleotide Archive (ENA) Portal. - See https://www.ebi.ac.uk/ena/portal/api/ for more information about the API - endpoints. - """ - - def __init__(self, options) -> None: - super().__init__(options) - - self.data_chunk_size = 10000 - self.data_url = "https://www.ebi.ac.uk/ena/portal/api/search" - self.ids = self.get_ids() - - def build_query(self, taxon_id: str = None) -> str: - """ - Returns a query to use for getting specific taxon ID accessions. - Some special characters must remain unquoted. - """ - - AND = " AND " - OR = " OR " - instrument_models = ( - "HiSeq X Five", - "HiSeq X Ten", - "Illumina Genome Analyzer II", - "Illumina Genome Analyzer IIx", - "Illumina Genome Analyzer", - "Illumina HiScanSQ", - "Illumina HiSeq 1000", - "Illumina HiSeq 1500", - "Illumina HiSeq 2000", - "Illumina HiSeq 2500", - "Illumina HiSeq 3000", - "Illumina HiSeq 4000", - "Illumina MiSeq", - "Illumina NovaSeq 6000", - "Ion Torrent Proton", - "Ion Torrent S5 XL", - "Ion Torrent S5", - "NextSeq 500", - "NextSeq 550", - ) - - instrument_models = OR.join((f'instrument_model="{im}"' for im in instrument_models)) - conditions = [ - # Relevant date fields: collection_date, collection_date_submitted, - # first_public, last_updated. - f"first_public >= {self.since}", - f"first_public <= {self.until}", - f"({instrument_models})", - 'library_source="TRANSCRIPTOMIC"', - 'library_strategy="RNA-Seq"', - ] - - if taxon_id: - conditions.append(f"tax_eq({taxon_id})") - elif self.keyword: - search_fields = ( - "assembly_software", - "bio_material", - "center_name", - "collected_by", - "experiment_title", - "host_body_site", - "instrument_model", - "instrument_platform", - "library_name", - "project_name", - "sample_title", - "sequencing_method", - "study_title", - ) - search_fields = OR.join( - (f'{sf}="*{self.keyword}*"' for sf in search_fields) - ) # Keyword regex. - conditions.append(f"({search_fields})") - elif self.organism: - # `host`: Natural (as opposed to laboratory) host to the organism from which sample - # was obtained. - # `host_scientific_name`: Scientific name of the natural (as opposed to laboratory) - # host to the organism from which sample was obtained. - # `scientific_name` Scientific name of the organism from which the sample was derived. - # Neither `host_scientific_name` nor `scientific_name` available for search. - # https://www.ebi.ac.uk/ena/portal/api/searchFields?dataPortal=ena&format=json&result=read_study - conditions.append(f'host="{self.organism}"') - - return quote(AND.join(conditions), safe='*()-="<>/ ') # Must remain unquoted. - - def collect_data(self) -> Set[str]: - """Gets new accessions from EBI ENA API.""" - accessions = set() - - if self.ids: - log.debug( - f"Getting RNA-Seq entries by taxon ID(s): " - f"{', '.join((str(idx) for idx in self.ids))} for [{self.since} - {self.until}] range." - ) - total = len(self.ids) - for idx, taxon_id in enumerate(self.ids): - if self.count and len(accessions) >= self.count: - break - - if total > 1: - log.debug(f"Getting entries for taxon ID {taxon_id}, {idx + 1} of {total}.") - accessions.update(self.fetch_data(taxon_id=taxon_id)) - elif self.keyword: - log.debug( - f'Getting RNA-Seq entries by "{self.keyword}" keyword ' - f"for [{self.since} - {self.until}] range." - ) - accessions.update(self.fetch_data()) - elif self.organism: - log.debug( - f'Getting entries by "{self.organism}" organism ' - f"for [{self.since} - {self.until}] range." - ) - accessions.update(self.fetch_data()) - - return accessions - - def fetch_data(self, taxon_id=None) -> Set[str]: - """ - Retrieves accessions from API search endpoint. - The API allows to set limit to 0 (get all in one request) but we do - it in a paginated fashion with `self.data_chunk_size` as a page size. - """ - - @retry(**self.retry_params) - def get_response(url, **kwargs): - """Gets response from an API endpoint.""" - return requests.post(url, **kwargs) - - accessions = set() - - fields = [ - "first_public", - "scientific_name", - "secondary_study_accession", - ] # For DRP/ERP/SRP-prefixed accessions. - data = { - "dataPortal": "ena", - # TODO(ark): add excludeAccessions/excludeAccessionType support. - "fields": ",".join(fields), # Use "all" to get all fields. - "format": "json", - "limit": self.data_chunk_size, - "offset": 0, - "query": self.build_query(taxon_id=taxon_id), - "result": "read_study", - "sortFields": fields, - } - - is_done = False - while not is_done: - log.debug( - f"Processing entries {data['offset'] + 1} - {data['offset'] + self.data_chunk_size}" - ) - entries = () - try: - response = get_response(self.data_url, data=data) - entries = response.json() - # TODO(ark): add `organism` when -o, --organism flag is used. - entries = ( - AccessionBacklogEntry.create_from_rnaseq_entry(entry) for entry in entries - ) - except JSONDecodeError: - is_done = True - except TypeError: - log.error(f"Couldn't get data from {self.data_url}. Response: {entries}") - data["offset"] += self.data_chunk_size - - if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) - accessions.update(entries) - - # Quit after getting a sufficient amount of accessions. - if self.count and len(accessions) >= self.count: - is_done = True - - return accessions - - def get_ids(self) -> List[str]: - """Returns a combined list of passed taxon IDs.""" - ids = set() - - if self.options["taxon_id"]: - ids.update(self.options["taxon_id"]) - - if self.options["taxon_ids_file"]: - with open(self.options["taxon_ids_file"]) as taxon_id_file: - ids.update((taxon_id.strip() for taxon_id in taxon_id_file.readlines())) - - return sorted(ids) + GatheredAccession.objects.bulk_create(entries) From 30434e042320e418774257cf494f49860be7f57e Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 13 Sep 2022 17:17:19 -0700 Subject: [PATCH 015/220] Add a TODO. --- foreman/data_refinery_foreman/gatherer/agents/rna_seq.py | 1 + 1 file changed, 1 insertion(+) diff --git a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py index f9497f3ba..f54ba570a 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py +++ b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py @@ -34,6 +34,7 @@ def build_query(self, taxon_id: str = None) -> str: AND = " AND " OR = " OR " + # TODO(ark): extract instrument models to a config file. instrument_models = ( "HiSeq X Five", "HiSeq X Ten", From 11cf3ea78695746fb5e636f7ab9466524ac5482a Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 13 Sep 2022 18:24:33 -0700 Subject: [PATCH 016/220] Sync recent changes. --- .../management/commands/gather_weekly_accessions.py | 7 ------- 1 file changed, 7 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_weekly_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_weekly_accessions.py index 35b27a5ab..648a71cce 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_weekly_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_weekly_accessions.py @@ -15,16 +15,9 @@ def handle(self, *args, **options): """Runs accession gathering.""" last_sunday = (datetime.now() - relativedelta(weekday=SU(-1))).strftime("%Y-%m-%d") - # TODO(ark): make the command process all sources within a single run. call_command( "gather_accessions", - "--microarray", - since=last_sunday, organism="homo sapiens", - ) - call_command( - "gather_accessions", - "--rna-seq", since=last_sunday, taxon_ids_file="config/rna_seq_taxon_ids.txt", ) From 811b77ff78d303316d1c10beaff18584122b8e4b Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 13 Sep 2022 18:37:09 -0700 Subject: [PATCH 017/220] Fix empty response issue. --- foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py index b5314302b..541bd86d2 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py @@ -92,7 +92,7 @@ def get_response(url, **kwargs): logger.debug(f"Processing entries {range_start} - {range_end}") response = get_response(self.DATA_URL, params=params) - entries = response.json().get("hits") + entries = response.json().get("hits", ()) if entries: entries = ( GatheredAccession.create_from_ma_ae_entry(entry, organism=self.organism) From 4ba5314bf1d7296b819227a9abbc05e7861bf6d6 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 14 Sep 2022 11:27:59 -0700 Subject: [PATCH 018/220] Add accession gathering logging. --- .../gatherer/agents/microarray_ae.py | 3 + .../gatherer/agents/microarray_geo.py | 3 + .../gatherer/agents/rna_seq.py | 3 + .../management/commands/gather_accessions.py | 67 +++++++++++-------- 4 files changed, 47 insertions(+), 29 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py index 541bd86d2..89fbe09f7 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py @@ -24,6 +24,9 @@ class MicroArrayExpressAccessionAgent(AccessionAgentBase): DATA_CHUNK_SIZE = 100 DATA_URL = "https://www.ebi.ac.uk/biostudies/api/v1/search" + def __str__(self): + return "MicroArray ArrayExpress accession agent" + def build_query(self) -> dict: """Returns a query dict for getting array/organism specific accessions.""" query_dict = { diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py index 975c715b3..afb243854 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py @@ -24,6 +24,9 @@ class MicroArrayGEOAccessionAgent(AccessionAgentBase): # Update URL once the original file is available again. DB_PATH = "data/microarray/GEOmetadb.sqlite" + def __str__(self): + return "MicroArray GEO accession agent" + def build_query(self) -> str: """Returns a query for getting GEO accessions from the local SQLite meta DB.""" tables = [ diff --git a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py index f54ba570a..cbb81c828 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py +++ b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py @@ -26,6 +26,9 @@ class RNASeqAccessionAgent(AccessionAgentBase): DATA_CHUNK_SIZE = 10000 DATA_URL = "https://www.ebi.ac.uk/ena/portal/api/search" + def __str__(self): + return "RNA-Seq accession agent" + def build_query(self, taxon_id: str = None) -> str: """ Returns a query to use for getting specific taxon ID accessions. diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 445245d3a..b770aaeb4 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -7,12 +7,13 @@ """ import argparse -import logging import re from django.core.management.base import BaseCommand +from django.template.defaultfilters import pluralize from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models import gathered_accession from data_refinery_common.models.gathered_accession import GatheredAccession from data_refinery_foreman.gatherer.agents.microarray_ae import MicroArrayExpressAccessionAgent from data_refinery_foreman.gatherer.agents.microarray_geo import MicroArrayGEOAccessionAgent @@ -89,13 +90,6 @@ def add_arguments(self, parser) -> None: type=str, help="Keyword to use for filtering.", ) - parser.add_argument( - "-lv", - "--log-verbose", - action="store_true", - default=False, - help="Enable verbose log output.", - ) parser.add_argument( "-ne", "--no-exclude-previous", @@ -141,14 +135,6 @@ def add_arguments(self, parser) -> None: help="Collect accessions made public before or on this date.", ) - def set_verbosity_level(self, options) -> None: - """Configures log verbosity level.""" - if options["log_verbose"]: - logger.addHandler(logging.StreamHandler()) - logger.setLevel(logging.DEBUG) - else: - logger.setLevel(logging.ERROR) - def validate_args(self, options) -> None: """Validates arguments.""" errors = list() @@ -210,7 +196,6 @@ def validate_args(self, options) -> None: def handle(self, *args, **options): """Creates agents and runs the accession gathering process.""" self.validate_args(options) - self.set_verbosity_level(options) agents = list() sources = options["source"] or self.DATA_SOURCES @@ -224,25 +209,49 @@ def handle(self, *args, **options): if self.DATA_SOURCE_MA_GEO in sources: agents.append(MicroArrayGEOAccessionAgent(options)) - entries = set() + gathered_accessions = set() + gathered_accessions_count = 0 for agent in agents: - entries.update(agent.collect_data()) + agent_accessions = agent.collect_data() + agent_accessions_count = len(agent_accessions) + + gathered_accessions.update(agent_accessions) + gathered_accessions_count += agent_accessions_count - entries = sorted( # Sort the resulting list. - (entry for entry in entries if self.RE_ACCESSION.match(entry.code)), - key=lambda entry: ( - self.RE_ACCESSION.match(entry.code).group(1), - int(self.RE_ACCESSION.match(entry.code).group(2)), + logger.info( + f"{agent} gathered {agent_accessions_count} " + f"new accession{pluralize(gathered_accessions_count)} since {options['since']}." + ) + + gathered_accessions = sorted( # Sort the resulting list. + (ga for ga in gathered_accessions if self.RE_ACCESSION.match(ga.code)), + key=lambda ga: ( + self.RE_ACCESSION.match(ga.code).group(1), + int(self.RE_ACCESSION.match(ga.code).group(2)), ), ) # Limit the number of output entries. - entries = entries[: options["count"]] if options["count"] else entries + gathered_accessions = ( + gathered_accessions[: options["count"]] if options["count"] else gathered_accessions + ) + + agents_count = len(agents) + logger.info( + f"{gathered_accessions_count} new accession{pluralize(gathered_accessions_count)} " + f"w{pluralize(gathered_accessions_count, 'as,ere')} gathered by {agents_count} " + f"accession agent{pluralize(agents_count)} since {options['since']}." + ) if options["dry_run"]: - if entries: - output = "\n".join((str(entry) for entry in entries)) + if gathered_accessions: + output = "\n".join((str(ga) for ga in gathered_accessions)) else: - output = "No accessions found." + output = "No new accessions gathered." print(output) else: - GatheredAccession.objects.bulk_create(entries) + GatheredAccession.objects.bulk_create(gathered_accessions) + added_accessions_count = len(gathered_accessions) + logger.info( + f"{added_accessions_count} new accession{pluralize(added_accessions_count)} " + f"w{pluralize(added_accessions_count, 'as,ere')} added to the database. " + ) From df14fe3d1e63991f681371e3ecf325e7a0d16edc Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 14 Sep 2022 18:52:57 -0700 Subject: [PATCH 019/220] Address review comments. --- .../migrations/0071_gatheredaccession.py | 2 +- .../models/gathered_accession.py | 72 +++++++------------ .../gatherer/agents/base.py | 5 +- .../gatherer/agents/microarray_ae.py | 21 ++++-- .../gatherer/agents/microarray_geo.py | 23 ++++-- .../gatherer/agents/rna_seq.py | 24 +++++-- .../management/commands/gather_accessions.py | 60 ++++++++-------- 7 files changed, 112 insertions(+), 95 deletions(-) diff --git a/common/data_refinery_common/migrations/0071_gatheredaccession.py b/common/data_refinery_common/migrations/0071_gatheredaccession.py index a1740d96e..65d192b59 100644 --- a/common/data_refinery_common/migrations/0071_gatheredaccession.py +++ b/common/data_refinery_common/migrations/0071_gatheredaccession.py @@ -22,7 +22,7 @@ class Migration(migrations.Migration): verbose_name="ID", ), ), - ("code", models.TextField(unique=True)), + ("accession_code", models.TextField(unique=True)), ("created_at", models.DateTimeField(auto_now_add=True)), ("last_modified_at", models.DateTimeField(auto_now=True)), ("organism", models.TextField()), diff --git a/common/data_refinery_common/models/gathered_accession.py b/common/data_refinery_common/models/gathered_accession.py index 04b084533..e56ed615c 100644 --- a/common/data_refinery_common/models/gathered_accession.py +++ b/common/data_refinery_common/models/gathered_accession.py @@ -10,7 +10,7 @@ class GatheredAccession(models.Model): class Meta: db_table = "gathered_accessions" - code = models.TextField(unique=True) + accession_code = models.TextField(unique=True) created_at = models.DateTimeField(auto_now_add=True) last_modified_at = models.DateTimeField(auto_now=True) organism = models.TextField() @@ -21,64 +21,44 @@ class Meta: def __eq__(self, other: object) -> bool: """Returns True if two objects are equal. Otherwise returns False.""" - return isinstance(other, GatheredAccession) and self.code == other.code + return isinstance(other, GatheredAccession) and self.accession_code == other.accession_code def __hash__(self) -> int: """Returns accession object unique hash value.""" - return hash(self.code) + return hash(self.accession_code) def __str__(self) -> str: """Returns accession default string representation.""" - return ", ".join((self.code, self.technology, self.source, str(self.published_date.date()))) - - @staticmethod - def create_from_ma_ae_entry(entry, organism=None): - """Creates accession object from MicroArray ArrayExpress entry.""" - accession = GatheredAccession() - accession.code = entry["accession"] - accession.source = "ebi_biostudies" - accession.technology = "microarray" - - if organism: - accession.organism = organism - if "release_date" in entry: - accession.published_date = timezone.make_aware( - datetime.strptime(entry["release_date"], "%Y-%m-%d") + return ", ".join( + ( + self.accession_code, + self.technology, + self.source, + str(self.published_date.date()), ) - - return accession + ) @staticmethod - def create_from_ma_geo_entry(entry): - """Creates accession object from MicroArray GEO meta DB entry.""" + def create_from_external_entry(data, source, technology, organism=None): + """Creates accession object from MicroArray ArrayExpress entry.""" accession = GatheredAccession() - accession.code = entry["gse"] - accession.source = "geo_meta_db" - accession.technology = "microarray" - - if "organism" in entry: - accession.organism = entry["organism"].lower() - if "submission_date" in entry: - accession.published_date = timezone.make_aware( - datetime.strptime(entry["submission_date"], "%Y-%m-%d") - ) + accession.accession_code = ( + data.get("accession") or data.get("gse") or data.get("secondary_study_accession") + ) - return accession + organism = data.get("organism") or data.get("scientific_name") or organism + if organism: + accession.organism = organism.lower() - @staticmethod - def create_from_rnaseq_entry(entry): - """Creates accession object from RNA-Seq entry.""" - accession = GatheredAccession() - accession.code = entry["secondary_study_accession"] - accession.source = "ebi_ena_portal" - accession.technology = "rna-seq" + published_date = ( + data.get("first_public") or data.get("release_date") or data.get("submission_date") + ) + accession.published_date = timezone.make_aware( + datetime.strptime(published_date, "%Y-%m-%d") + ) - if "scientific_name" in entry: - accession.organism = entry["scientific_name"].lower() - if "first_public" in entry: - accession.published_date = timezone.make_aware( - datetime.strptime(entry["first_public"], "%Y-%m-%d") - ) + accession.source = source + accession.technology = technology return accession diff --git a/foreman/data_refinery_foreman/gatherer/agents/base.py b/foreman/data_refinery_foreman/gatherer/agents/base.py index 3754a4068..818bbf72c 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/base.py +++ b/foreman/data_refinery_foreman/gatherer/agents/base.py @@ -66,7 +66,10 @@ def populate_previous_accessions(self) -> None: # Gathered accessions. self.previous_accessions.update( - (entry["code"] for entry in GatheredAccession.objects.values("code")) + ( + entry["accession_code"] + for entry in GatheredAccession.objects.values("accession_code") + ) ) # Surveyed accessions. diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py index 541bd86d2..3bfcf08fe 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py @@ -1,5 +1,7 @@ -"""MicroArray ArrayExpress accession gathering automation. -Data source: https://www.ebi.ac.uk/biostudies/help""" +""" +MicroArray ArrayExpress accession gathering automation. +Data source: https://www.ebi.ac.uk/biostudies/help +""" from typing import List, Set @@ -13,7 +15,7 @@ logger = get_and_configure_logger(__name__) -class MicroArrayExpressAccessionAgent(AccessionAgentBase): +class AEAgent(AccessionAgentBase): """ MicroArray ArrayExpress accession gathering agent. The data is fetched from the BioStudies database. See https://www.ebi.ac.uk/biostudies/help and @@ -23,6 +25,9 @@ class MicroArrayExpressAccessionAgent(AccessionAgentBase): DATA_CHUNK_SIZE = 100 DATA_URL = "https://www.ebi.ac.uk/biostudies/api/v1/search" + SOURCE = "ebi-biostudies" + SOURCE_NAME = "microarray-ae" + TECHNOLOGY = "microarray" def build_query(self) -> dict: """Returns a query dict for getting array/organism specific accessions.""" @@ -95,7 +100,9 @@ def get_response(url, **kwargs): entries = response.json().get("hits", ()) if entries: entries = ( - GatheredAccession.create_from_ma_ae_entry(entry, organism=self.organism) + GatheredAccession.create_from_external_entry( + entry, self.SOURCE, self.TECHNOLOGY, organism=self.organism + ) for entry in entries ) params["page"] += 1 @@ -103,7 +110,11 @@ def get_response(url, **kwargs): is_done = True if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) + entries = ( + entry + for entry in entries + if entry.accession_code not in self.previous_accessions + ) accessions.update(entries) # Quit after getting a sufficient amount of accessions. diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py index 975c715b3..2500bcec5 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py @@ -1,5 +1,8 @@ -"""MicroArray GEO accession gathering automation. -Data source: local SQLite meta DB from https://www.bioconductor.org/packages/release/bioc/html/GEOmetadb.html""" +""" +MicroArray GEO accession gathering automation. +Data source: local SQLite meta DB from +https://www.bioconductor.org/packages/release/bioc/html/GEOmetadb.html +""" import os import re @@ -13,7 +16,7 @@ logger = get_and_configure_logger(__name__) -class MicroArrayGEOAccessionAgent(AccessionAgentBase): +class GEOAgent(AccessionAgentBase): """ MicroArray GEO accession gathering agent. The data is fetched from a local SQLite GEO meta database. @@ -23,6 +26,9 @@ class MicroArrayGEOAccessionAgent(AccessionAgentBase): # Implement syncing procedure. # Update URL once the original file is available again. DB_PATH = "data/microarray/GEOmetadb.sqlite" + SOURCE = "geo-meta-db" + SOURCE_NAME = "microarray-geo" + TECHNOLOGY = "microarray" def build_query(self) -> str: """Returns a query for getting GEO accessions from the local SQLite meta DB.""" @@ -101,10 +107,17 @@ def match_keyword(row): entries = filter(match_keyword, entries) entries = ({key.lower(): entry[key] for key in entry.keys()} for entry in entries) - entries = set((GatheredAccession.create_from_ma_geo_entry(entry) for entry in entries)) + entries = set( + ( + GatheredAccession.create_from_external_entry(entry, self.SOURCE, self.TECHNOLOGY) + for entry in entries + ) + ) if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) + entries = ( + entry for entry in entries if entry.accession_code not in self.previous_accessions + ) accessions.update(entries) return accessions diff --git a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py index f54ba570a..577f815b8 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py +++ b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py @@ -1,5 +1,7 @@ -"""RNA-Seq accession gathering automation. -Data source: https://www.ebi.ac.uk/ena/portal/api/""" +""" +RNA-Seq accession gathering automation. +Data source: https://www.ebi.ac.uk/ena/portal/api/ +""" from json.decoder import JSONDecodeError from typing import List, Set @@ -15,7 +17,7 @@ logger = get_and_configure_logger(__name__) -class RNASeqAccessionAgent(AccessionAgentBase): +class RNASeqAgent(AccessionAgentBase): """ RNA-Seq accession gathering agent. The data is fetched from The European Nucleotide Archive (ENA) Portal. @@ -25,6 +27,9 @@ class RNASeqAccessionAgent(AccessionAgentBase): DATA_CHUNK_SIZE = 10000 DATA_URL = "https://www.ebi.ac.uk/ena/portal/api/search" + SOURCE = "ebi-ena-portal" + SOURCE_NAME = "rna-seq" + TECHNOLOGY = "rna-seq" def build_query(self, taxon_id: str = None) -> str: """ @@ -174,7 +179,12 @@ def get_response(url, **kwargs): try: response = get_response(self.DATA_URL, data=data) entries = response.json() - entries = (GatheredAccession.create_from_rnaseq_entry(entry) for entry in entries) + entries = ( + GatheredAccession.create_from_external_entry( + entry, self.SOURCE, self.TECHNOLOGY + ) + for entry in entries + ) except JSONDecodeError: is_done = True except TypeError: @@ -182,7 +192,11 @@ def get_response(url, **kwargs): data["offset"] += self.DATA_CHUNK_SIZE if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) + entries = ( + entry + for entry in entries + if entry.accession_code not in self.previous_accessions + ) accessions.update(entries) # Quit after getting a sufficient amount of accessions. diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 445245d3a..2b073ef45 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -11,12 +11,13 @@ import re from django.core.management.base import BaseCommand +from django.db.utils import IntegrityError from data_refinery_common.logging import get_and_configure_logger from data_refinery_common.models.gathered_accession import GatheredAccession -from data_refinery_foreman.gatherer.agents.microarray_ae import MicroArrayExpressAccessionAgent -from data_refinery_foreman.gatherer.agents.microarray_geo import MicroArrayGEOAccessionAgent -from data_refinery_foreman.gatherer.agents.rna_seq import RNASeqAccessionAgent +from data_refinery_foreman.gatherer.agents.microarray_ae import AEAgent +from data_refinery_foreman.gatherer.agents.microarray_geo import GEOAgent +from data_refinery_foreman.gatherer.agents.rna_seq import RNASeqAgent logger = get_and_configure_logger(__name__) @@ -24,11 +25,8 @@ class Command(BaseCommand): """Creates agents and runs actual accession gathering.""" - DATA_SOURCE_MA_AE = "microarray-ae" - DATA_SOURCE_MA_GEO = "microarray-geo" - DATA_SOURCE_RNA_SEQ = "rna-seq" - DATA_SOURCES = (DATA_SOURCE_MA_AE, DATA_SOURCE_MA_GEO, DATA_SOURCE_RNA_SEQ) - + DATA_AGENTS = (AEAgent, GEOAgent, RNASeqAgent) + DATA_SOURCE_NAMES = [agent.SOURCE_NAME for agent in DATA_AGENTS] RE_ACCESSION = re.compile(r"(\D+)(\d+)") RE_DATE = re.compile(r"\d{4}-\d{2}-\d{2}") @@ -164,43 +162,43 @@ def validate_args(self, options) -> None: keyword = options["keyword"] organism = options["organism"] - sources = options["source"] or self.DATA_SOURCES + source_names = options["source"] or self.DATA_SOURCE_NAMES - for source in sources: - if source in self.DATA_SOURCES: + for source_name in source_names: + if source_name in self.DATA_SOURCE_NAMES: continue errors.append( - f"Unknown source: {source}. Supported sources: {', '.join(self.DATA_SOURCES)}" + f"Unknown source: {source_name}. Supported sources: {', '.join(self.DATA_SOURCE_NAMES)}" ) - if self.DATA_SOURCE_MA_AE in sources: + if AEAgent.SOURCE_NAME in source_names: ids = options["ae_id"] or options["ae_ids_file"] if not (ids or keyword or organism): errors.append( ( "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " "ArrayExpress ID(s) [--ae-id, --ae-ids-file] must be specified for " - f"'{self.DATA_SOURCE_MA_AE}' source." + f"'{AEAgent.SOURCE_NAME}' source." ) ) - if self.DATA_SOURCE_MA_GEO in sources: + if GEOAgent.SOURCE_NAME in source_names: ids = options["gpl_id"] or options["gpl_ids_file"] if not (ids or keyword or organism): errors.append( ( "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " "GEO platform ID(s) [--gpl-id, --gpl-ids-file] must be specified for " - f"'{self.DATA_SOURCE_MA_GEO}' source." + f"'{GEOAgent.SOURCE_NAME}' source." ) ) - if self.DATA_SOURCE_RNA_SEQ in sources: + if RNASeqAgent.SOURCE_NAME in source_names: ids = options["taxon_id"] or options["taxon_ids_file"] if not (ids or keyword or organism): errors.append( ( "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] " "or taxon ID(s) [--taxon-id, --taxon-ids-file] must be specified for " - f"'{self.DATA_SOURCE_RNA_SEQ}' source." + f"'{RNASeqAgent.SOURCE_NAME}' source." ) ) @@ -213,26 +211,21 @@ def handle(self, *args, **options): self.set_verbosity_level(options) agents = list() - sources = options["source"] or self.DATA_SOURCES - - if self.DATA_SOURCE_RNA_SEQ in sources: - agents.append(RNASeqAccessionAgent(options)) - - if self.DATA_SOURCE_MA_AE in sources: - agents.append(MicroArrayExpressAccessionAgent(options)) - - if self.DATA_SOURCE_MA_GEO in sources: - agents.append(MicroArrayGEOAccessionAgent(options)) + sources_names = options["source"] or self.DATA_SOURCE_NAMES + for cls in self.DATA_AGENTS: + if cls.SOURCE_NAME not in sources_names: + continue + agents.append(cls(options)) entries = set() for agent in agents: entries.update(agent.collect_data()) entries = sorted( # Sort the resulting list. - (entry for entry in entries if self.RE_ACCESSION.match(entry.code)), + (entry for entry in entries if self.RE_ACCESSION.match(entry.accession_code)), key=lambda entry: ( - self.RE_ACCESSION.match(entry.code).group(1), - int(self.RE_ACCESSION.match(entry.code).group(2)), + self.RE_ACCESSION.match(entry.accession_code).group(1), + int(self.RE_ACCESSION.match(entry.accession_code).group(2)), ), ) # Limit the number of output entries. @@ -245,4 +238,7 @@ def handle(self, *args, **options): output = "No accessions found." print(output) else: - GatheredAccession.objects.bulk_create(entries) + try: + GatheredAccession.objects.bulk_create(entries) + except IntegrityError as e: + logger.exception(f"Could not save new accessions to the database: {e}") From c4b43eecc479255a40902707bd026eeacff95788 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 21 Sep 2022 11:05:24 -0700 Subject: [PATCH 020/220] Rename agent files. --- .../gatherer/agents/{microarray_ae.py => ae_agent.py} | 0 .../gatherer/agents/{microarray_geo.py => geo_agent.py} | 0 .../gatherer/agents/{rna_seq.py => rnaseq_agent.py} | 0 .../gatherer/management/commands/gather_accessions.py | 6 +++--- 4 files changed, 3 insertions(+), 3 deletions(-) rename foreman/data_refinery_foreman/gatherer/agents/{microarray_ae.py => ae_agent.py} (100%) rename foreman/data_refinery_foreman/gatherer/agents/{microarray_geo.py => geo_agent.py} (100%) rename foreman/data_refinery_foreman/gatherer/agents/{rna_seq.py => rnaseq_agent.py} (100%) diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py b/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py similarity index 100% rename from foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py rename to foreman/data_refinery_foreman/gatherer/agents/ae_agent.py diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py b/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py similarity index 100% rename from foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py rename to foreman/data_refinery_foreman/gatherer/agents/geo_agent.py diff --git a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py similarity index 100% rename from foreman/data_refinery_foreman/gatherer/agents/rna_seq.py rename to foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 2b073ef45..554b74350 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -15,9 +15,9 @@ from data_refinery_common.logging import get_and_configure_logger from data_refinery_common.models.gathered_accession import GatheredAccession -from data_refinery_foreman.gatherer.agents.microarray_ae import AEAgent -from data_refinery_foreman.gatherer.agents.microarray_geo import GEOAgent -from data_refinery_foreman.gatherer.agents.rna_seq import RNASeqAgent +from data_refinery_foreman.gatherer.agents.ae_agent import AEAgent +from data_refinery_foreman.gatherer.agents.geo_agent import GEOAgent +from data_refinery_foreman.gatherer.agents.rnaseq_agent import RNASeqAgent logger = get_and_configure_logger(__name__) From fc813da8e93864174f7ddd2da962c6a9208002a5 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 21 Sep 2022 12:15:48 -0700 Subject: [PATCH 021/220] Add accession gathering logging. --- .../gatherer/agents/ae_agent.py | 3 + .../gatherer/agents/geo_agent.py | 3 + .../gatherer/agents/rnaseq_agent.py | 3 + .../management/commands/gather_accessions.py | 67 +++++++++++-------- 4 files changed, 47 insertions(+), 29 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py b/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py index 3bfcf08fe..4328f0713 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py +++ b/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py @@ -29,6 +29,9 @@ class AEAgent(AccessionAgentBase): SOURCE_NAME = "microarray-ae" TECHNOLOGY = "microarray" + def __str__(self): + return "MicroArray ArrayExpress accession agent" + def build_query(self) -> dict: """Returns a query dict for getting array/organism specific accessions.""" query_dict = { diff --git a/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py b/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py index 2500bcec5..22a3bd0bd 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py +++ b/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py @@ -30,6 +30,9 @@ class GEOAgent(AccessionAgentBase): SOURCE_NAME = "microarray-geo" TECHNOLOGY = "microarray" + def __str__(self): + return "MicroArray GEO accession agent" + def build_query(self) -> str: """Returns a query for getting GEO accessions from the local SQLite meta DB.""" tables = [ diff --git a/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py index 577f815b8..3fcb87fbb 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py +++ b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py @@ -31,6 +31,9 @@ class RNASeqAgent(AccessionAgentBase): SOURCE_NAME = "rna-seq" TECHNOLOGY = "rna-seq" + def __str__(self): + return "RNA-Seq accession agent" + def build_query(self, taxon_id: str = None) -> str: """ Returns a query to use for getting specific taxon ID accessions. diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 554b74350..f3f644a91 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -7,13 +7,14 @@ """ import argparse -import logging import re from django.core.management.base import BaseCommand from django.db.utils import IntegrityError +from django.template.defaultfilters import pluralize from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models import gathered_accession from data_refinery_common.models.gathered_accession import GatheredAccession from data_refinery_foreman.gatherer.agents.ae_agent import AEAgent from data_refinery_foreman.gatherer.agents.geo_agent import GEOAgent @@ -87,13 +88,6 @@ def add_arguments(self, parser) -> None: type=str, help="Keyword to use for filtering.", ) - parser.add_argument( - "-lv", - "--log-verbose", - action="store_true", - default=False, - help="Enable verbose log output.", - ) parser.add_argument( "-ne", "--no-exclude-previous", @@ -139,14 +133,6 @@ def add_arguments(self, parser) -> None: help="Collect accessions made public before or on this date.", ) - def set_verbosity_level(self, options) -> None: - """Configures log verbosity level.""" - if options["log_verbose"]: - logger.addHandler(logging.StreamHandler()) - logger.setLevel(logging.DEBUG) - else: - logger.setLevel(logging.ERROR) - def validate_args(self, options) -> None: """Validates arguments.""" errors = list() @@ -208,7 +194,6 @@ def validate_args(self, options) -> None: def handle(self, *args, **options): """Creates agents and runs the accession gathering process.""" self.validate_args(options) - self.set_verbosity_level(options) agents = list() sources_names = options["source"] or self.DATA_SOURCE_NAMES @@ -217,28 +202,52 @@ def handle(self, *args, **options): continue agents.append(cls(options)) - entries = set() + gathered_accessions = set() + gathered_accessions_count = 0 for agent in agents: - entries.update(agent.collect_data()) + agent_accessions = agent.collect_data() + agent_accessions_count = len(agent_accessions) + + gathered_accessions.update(agent_accessions) + gathered_accessions_count += agent_accessions_count - entries = sorted( # Sort the resulting list. - (entry for entry in entries if self.RE_ACCESSION.match(entry.accession_code)), - key=lambda entry: ( - self.RE_ACCESSION.match(entry.accession_code).group(1), - int(self.RE_ACCESSION.match(entry.accession_code).group(2)), + logger.info( + f"{agent} gathered {agent_accessions_count} " + f"new accession{pluralize(gathered_accessions_count)} since {options['since']}." + ) + + gathered_accessions = sorted( # Sort the resulting list. + (ga for ga in gathered_accessions if self.RE_ACCESSION.match(ga.code)), + key=lambda ga: ( + self.RE_ACCESSION.match(ga.code).group(1), + int(self.RE_ACCESSION.match(ga.code).group(2)), ), ) # Limit the number of output entries. - entries = entries[: options["count"]] if options["count"] else entries + gathered_accessions = ( + gathered_accessions[: options["count"]] if options["count"] else gathered_accessions + ) + + agents_count = len(agents) + logger.info( + f"{gathered_accessions_count} new accession{pluralize(gathered_accessions_count)} " + f"w{pluralize(gathered_accessions_count, 'as,ere')} gathered by {agents_count} " + f"accession agent{pluralize(agents_count)} since {options['since']}." + ) if options["dry_run"]: - if entries: - output = "\n".join((str(entry) for entry in entries)) + if gathered_accessions: + output = "\n".join((str(ga) for ga in gathered_accessions)) else: - output = "No accessions found." + output = "No new accessions gathered." print(output) else: try: - GatheredAccession.objects.bulk_create(entries) + GatheredAccession.objects.bulk_create(gathered_accessions) + added_accessions_count = len(gathered_accessions) + logger.info( + f"{added_accessions_count} new accession{pluralize(added_accessions_count)} " + f"w{pluralize(added_accessions_count, 'as,ere')} added to the database. " + ) except IntegrityError as e: logger.exception(f"Could not save new accessions to the database: {e}") From a3bb0f2348b1d96d10d2bf2a5b3e4a4b47d96663 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 22 Sep 2022 16:59:59 -0700 Subject: [PATCH 022/220] Add automatic accession command. Exclude `SurveyJob` accessions instead of `Experiment` accessions. --- .../management/commands/automatic_survey.py | 72 +++++++++++++++++++ .../gatherer/agents/base.py | 11 +-- 2 files changed, 75 insertions(+), 8 deletions(-) create mode 100644 foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py diff --git a/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py b/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py new file mode 100644 index 000000000..02063e11b --- /dev/null +++ b/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py @@ -0,0 +1,72 @@ +""" +Automatic survey command. +""" + +from django.core.management.base import BaseCommand +from django.template.defaultfilters import pluralize +from django.utils import timezone + +from dateutil.relativedelta import relativedelta + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models import GatheredAccession, SurveyedAccession, SurveyJob +from data_refinery_foreman.surveyor.management.commands.surveyor_dispatcher import ( + queue_surveyor_for_accession, +) + +logger = get_and_configure_logger(__name__) + + +class Command(BaseCommand): + def add_arguments(self, parser): + parser.add_argument("-d", "--days", help="The time window in days.", type=int, default=7) + parser.add_argument( + "-q", + "--quota", + help="The number of accessions to survey per the time window.", + type=int, + default=10, + ) + + def handle(self, *args, **options): + create_survey_jobs(options["days"], options["quota"]) + + +def create_survey_jobs(days, quota): + """Automatically creates survey jobs based on the passed time window requirements.""" + surveyed_accessions = ( + sa["accession_code"] for sa in SurveyedAccession.objects.values("accession_code") + ) + gathered_accessions = [ + ga["accession_code"] + for ga in GatheredAccession.objects.exclude(accession_code__in=surveyed_accessions) + .order_by("created_at") + .values("accession_code") + ] + + time_window_survey_jobs = SurveyJob.objects.filter( + created_at__gt=timezone.now() - relativedelta(days=days) + ) + + remaining_quota = quota - time_window_survey_jobs.count() + if remaining_quota > 0: + queued_accessions = list() + for accession_code in gathered_accessions[:remaining_quota]: + try: + queue_surveyor_for_accession(accession_code) + queued_accessions.append(accession_code) + except Exception as e: + logger.error(f"Couldn't queue accession {accession_code} due to: {e}") + + queued_accession_count = len(queued_accessions) + logger.info( + f"Queued {queued_accession_count} accession{pluralize(queued_accession_count)} " + f"based on {quota} accession{pluralize(quota)} per {days} " + f"day{pluralize(days)} quota." + ) + SurveyedAccession.objects.bulk_create( + ( + SurveyedAccession(accession_code=accession_code) + for accession_code in queued_accessions + ) + ) diff --git a/foreman/data_refinery_foreman/gatherer/agents/base.py b/foreman/data_refinery_foreman/gatherer/agents/base.py index 818bbf72c..bf730fb86 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/base.py +++ b/foreman/data_refinery_foreman/gatherer/agents/base.py @@ -8,8 +8,7 @@ from urllib3.exceptions import ProtocolError from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models.experiment import Experiment -from data_refinery_common.models.gathered_accession import GatheredAccession +from data_refinery_common.models import GatheredAccession, SurveyedAccession logger = get_and_configure_logger(__name__) @@ -60,7 +59,7 @@ def get_ids(self): pass def populate_previous_accessions(self) -> None: - """Populates previous accession set from a provided excluded ids file.""" + """Populates previous accession set from gathered/surveyed accessions.""" if not self.options["exclude_previous"] or self.previous_accessions: return @@ -73,10 +72,6 @@ def populate_previous_accessions(self) -> None: ) # Surveyed accessions. - experiments = Experiment.objects.values("accession_code", "alternate_accession_code") self.previous_accessions.update( - (experiment["accession_code"] for experiment in experiments) - ) - self.previous_accessions.update( - (experiment["alternate_accession_code"] for experiment in experiments) + (sa["accession_code"] for sa in SurveyedAccession.objects.values("accession_code")) ) From d27cc5e1b3fd447af59902e274cb12e63a56306a Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 27 Sep 2022 12:15:22 -0700 Subject: [PATCH 023/220] Address PR comments. --- .../management/commands/automatic_survey.py | 76 ++++++++++++------- 1 file changed, 49 insertions(+), 27 deletions(-) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py b/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py index 02063e11b..6dc1273ac 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py @@ -19,7 +19,7 @@ class Command(BaseCommand): def add_arguments(self, parser): - parser.add_argument("-d", "--days", help="The time window in days.", type=int, default=7) + parser.add_argument("-d", "--days", help="The time window in days.", type=int, default=1) parser.add_argument( "-q", "--quota", @@ -34,39 +34,61 @@ def handle(self, *args, **options): def create_survey_jobs(days, quota): """Automatically creates survey jobs based on the passed time window requirements.""" - surveyed_accessions = ( - sa["accession_code"] for sa in SurveyedAccession.objects.values("accession_code") - ) + + remaining_quota = get_remaining_quota(days, quota) + if not remaining_quota: + return + gathered_accessions = [ ga["accession_code"] - for ga in GatheredAccession.objects.exclude(accession_code__in=surveyed_accessions) - .order_by("created_at") - .values("accession_code") + for ga in GatheredAccession.objects.order_by("created_at").values("accession_code") ] - time_window_survey_jobs = SurveyJob.objects.filter( - created_at__gt=timezone.now() - relativedelta(days=days) - ) + batch_start = 0 + batch_size = 1000 + queued_accessions = list() + while True: + batch_accessions = gathered_accessions[batch_start : batch_start + batch_size] + if not batch_accessions: + break - remaining_quota = quota - time_window_survey_jobs.count() - if remaining_quota > 0: - queued_accessions = list() - for accession_code in gathered_accessions[:remaining_quota]: + surveyed_accessions = ( + sa["accession_code"] + for sa in SurveyedAccession.objects.filter(accession_code__in=batch_accessions).values( + "accession_code" + ) + ) + + for accession_code in set(batch_accessions) - set(surveyed_accessions): try: queue_surveyor_for_accession(accession_code) - queued_accessions.append(accession_code) except Exception as e: logger.error(f"Couldn't queue accession {accession_code} due to: {e}") + else: + queued_accessions.append(accession_code) + if len(queued_accessions) == remaining_quota: + break - queued_accession_count = len(queued_accessions) - logger.info( - f"Queued {queued_accession_count} accession{pluralize(queued_accession_count)} " - f"based on {quota} accession{pluralize(quota)} per {days} " - f"day{pluralize(days)} quota." - ) - SurveyedAccession.objects.bulk_create( - ( - SurveyedAccession(accession_code=accession_code) - for accession_code in queued_accessions - ) - ) + if len(queued_accessions) == remaining_quota: + break + + batch_start += batch_size + + queued_accession_count = len(queued_accessions) + logger.info( + f"Queued {queued_accession_count} accession{pluralize(queued_accession_count)} " + f"based on {quota} accession{pluralize(quota)} per {days} " + f"day{pluralize(days)} quota." + ) + SurveyedAccession.objects.bulk_create( + (SurveyedAccession(accession_code=accession_code) for accession_code in queued_accessions) + ) + + +def get_remaining_quota(days, quota): + """Returns the remaining quota or 0 if nothing left.""" + time_window_survey_jobs = SurveyJob.objects.filter( + created_at__gt=timezone.now() - relativedelta(days=days) + ) + + return max(0, quota - time_window_survey_jobs.count()) From 140470b8d5ccf2f64ee6769aabbf0fd5a4463b1a Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 27 Sep 2022 17:18:38 -0700 Subject: [PATCH 024/220] Add indexes to `GatheredAccession` model. --- .../migrations/0072_auto_20220927_2235.py | 21 +++++++++++++++++++ .../models/gathered_accession.py | 4 ++++ 2 files changed, 25 insertions(+) create mode 100644 common/data_refinery_common/migrations/0072_auto_20220927_2235.py diff --git a/common/data_refinery_common/migrations/0072_auto_20220927_2235.py b/common/data_refinery_common/migrations/0072_auto_20220927_2235.py new file mode 100644 index 000000000..06e9451a8 --- /dev/null +++ b/common/data_refinery_common/migrations/0072_auto_20220927_2235.py @@ -0,0 +1,21 @@ +# Generated by Django 3.2.7 on 2022-09-27 22:35 + +from django.db import migrations, models + + +class Migration(migrations.Migration): + + dependencies = [ + ("data_refinery_common", "0071_gatheredaccession"), + ] + + operations = [ + migrations.AddIndex( + model_name="gatheredaccession", + index=models.Index(fields=["accession_code"], name="gathered_ac_accessi_5dde9a_idx"), + ), + migrations.AddIndex( + model_name="gatheredaccession", + index=models.Index(fields=["created_at"], name="gathered_ac_created_2efd0f_idx"), + ), + ] diff --git a/common/data_refinery_common/models/gathered_accession.py b/common/data_refinery_common/models/gathered_accession.py index e56ed615c..1d71de204 100644 --- a/common/data_refinery_common/models/gathered_accession.py +++ b/common/data_refinery_common/models/gathered_accession.py @@ -9,6 +9,10 @@ class GatheredAccession(models.Model): class Meta: db_table = "gathered_accessions" + indexes = ( + models.Index(fields=("accession_code",)), + models.Index(fields=("created_at",)), + ) accession_code = models.TextField(unique=True) created_at = models.DateTimeField(auto_now_add=True) From 16b4cd044cdf8e476a16755f5c098bdf3133d25f Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 29 Sep 2022 08:42:39 -0700 Subject: [PATCH 025/220] Fix shell check errors. Add quotes around $OPTARG. --- foreman/test_survey.sh | 4 ++-- scripts/format_batch_with_env.sh | 8 ++++---- scripts/update_my_docker_images.sh | 4 ++-- 3 files changed, 8 insertions(+), 8 deletions(-) diff --git a/foreman/test_survey.sh b/foreman/test_survey.sh index c77ba6a6d..55e662174 100755 --- a/foreman/test_survey.sh +++ b/foreman/test_survey.sh @@ -12,10 +12,10 @@ EOF while getopts ":s:a:h" opt; do case $opt in s) - export SURVEYOR=$OPTARG + export SURVEYOR="$OPTARG" ;; a) - export ACCESSION=$OPTARG + export ACCESSION="$OPTARG" ;; h) print_description diff --git a/scripts/format_batch_with_env.sh b/scripts/format_batch_with_env.sh index 1977b0426..5ab5bd386 100755 --- a/scripts/format_batch_with_env.sh +++ b/scripts/format_batch_with_env.sh @@ -25,16 +25,16 @@ print_options() { while getopts ":p:e:o:v:h" opt; do case $opt in p) - export project=$OPTARG + export project="$OPTARG" ;; e) - export env=$OPTARG + export env="$OPTARG" ;; o) - export output_dir=$OPTARG + export output_dir="$OPTARG" ;; v) - export system_version=$OPTARG + export system_version="$OPTARG" ;; h) print_description diff --git a/scripts/update_my_docker_images.sh b/scripts/update_my_docker_images.sh index 32950fda0..025e9ad1f 100755 --- a/scripts/update_my_docker_images.sh +++ b/scripts/update_my_docker_images.sh @@ -38,10 +38,10 @@ EOF while getopts ":d:v:ah" opt; do case $opt in d) - export DOCKERHUB_REPO=$OPTARG + export DOCKERHUB_REPO="$OPTARG" ;; v) - export SYSTEM_VERSION=$OPTARG + export SYSTEM_VERSION="$OPTARG" ;; a) AFFYMETRIX=true From fef0e6fd4b07eccbdfc25a6d5860000b41203281 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 3 Oct 2022 16:15:57 -0700 Subject: [PATCH 026/220] Update terraform config in order to make it runnable on arm64. - Migrate `template_file` to `templatefile` - Inline user_data templates. - Fix s3 backend configuration. - Set postgres version to 11.16. - Change DB `name` to `db_name`. --- ami/instance.tf | 12 ++- ami/user-data-script.tf | 16 ---- infrastructure/backend.tf | 10 +-- infrastructure/batch.tf | 14 +++- infrastructure/database.tf | 34 ++++---- infrastructure/instances.tf | 162 ++++++++++++++---------------------- infrastructure/variables.tf | 2 +- 7 files changed, 103 insertions(+), 147 deletions(-) diff --git a/ami/instance.tf b/ami/instance.tf index 620b13803..80680f351 100644 --- a/ami/instance.tf +++ b/ami/instance.tf @@ -26,7 +26,11 @@ resource "aws_instance" "ubuntu-ami-template-instance" { # Our instance-user-data.sh script is built by Terraform at # apply-time so that it can put additional files onto the # instance. For more information see the definition of this resource. - user_data = data.template_file.ubuntu_instance_user_data.rendered + user_data = templatefile("ubuntu-instance-user-data.tpl.sh", + { + docker_apt_key = data.local_file.docker_apt_key.content + } + ) subnet_id = data.aws_subnet.ccdl_dev_subnet.id associate_public_ip_address = true @@ -46,7 +50,11 @@ resource "aws_instance" "ecs-ami-template-instance" { # Our instance-user-data.sh script is built by Terraform at # apply-time so that it can put additional files onto the # instance. For more information see the definition of this resource. - user_data = data.template_file.ecs_instance_user_data.rendered + user_data = templatefile("ecs-instance-user-data.tpl.sh", + { + docker_apt_key = data.local_file.docker_apt_key.content + } + ) subnet_id = data.aws_subnet.ccdl_dev_subnet.id associate_public_ip_address = true diff --git a/ami/user-data-script.tf b/ami/user-data-script.tf index d5d0718e3..585beacbe 100644 --- a/ami/user-data-script.tf +++ b/ami/user-data-script.tf @@ -2,19 +2,3 @@ data "local_file" "docker_apt_key" { filename = "docker-apt-key.gpg" } - -data "template_file" "ubuntu_instance_user_data" { - template = file("ubuntu-instance-user-data.tpl.sh") - - vars = { - docker_apt_key = data.local_file.docker_apt_key.content - } -} - -data "template_file" "ecs_instance_user_data" { - template = file("ecs-instance-user-data.tpl.sh") - - vars = { - docker_apt_key = data.local_file.docker_apt_key.content - } -} diff --git a/infrastructure/backend.tf b/infrastructure/backend.tf index 02e25fc39..294f50ef7 100644 --- a/infrastructure/backend.tf +++ b/infrastructure/backend.tf @@ -1,16 +1,8 @@ terraform { backend "s3" { - # Terraform will prompt the user for the other keys. - region = "us-east-1" - } -} - -data "terraform_remote_state" "network" { - backend = "s3" - config = { bucket = "refinebio-tfstate-deploy-${var.stage}" + encrypt = true key = "terraform-${var.user}.tfstate" region = "us-east-1" - encrypt = true } } diff --git a/infrastructure/batch.tf b/infrastructure/batch.tf index 8dbbebd94..ae1ab8216 100644 --- a/infrastructure/batch.tf +++ b/infrastructure/batch.tf @@ -16,7 +16,19 @@ module "batch" { data_refinery_keypair = aws_key_pair.data_refinery data_refinery_worker_security_group = aws_security_group.data_refinery_worker - data_refinery_worker_user_data = data.template_file.worker_script_smusher.rendered + data_refinery_worker_user_data = templatefile( + "workers-configuration/workers-instance-user-data.tpl.sh", + { + database_host = aws_instance.pg_bouncer.private_ip + database_name = aws_db_instance.postgres_db.name + database_password = var.database_password + database_port = var.database_port + database_user = var.database_user + region = var.region + stage = var.stage + user = var.user + } + ) data_refinery_worker_ami = var.worker_ami user = var.user diff --git a/infrastructure/database.tf b/infrastructure/database.tf index 8f4f8520e..503fcf75b 100644 --- a/infrastructure/database.tf +++ b/infrastructure/database.tf @@ -137,11 +137,11 @@ resource "aws_db_instance" "postgres_db" { allocated_storage = 100 storage_type = "gp2" engine = "postgres" - engine_version = "11.1" + engine_version = "11.16" allow_major_version_upgrade = true auto_minor_version_upgrade = false instance_class = "db.${var.database_instance_type}" - name = "data_refinery" + db_name = "data_refinery" port = var.database_hidden_port username = var.database_user password = var.database_password @@ -182,7 +182,19 @@ resource "aws_instance" "pg_bouncer" { # Our instance-user-data.sh script is built by Terraform at # apply-time so that it can put additional files onto the # instance. For more information see the definition of this resource. - user_data = data.template_file.pg_bouncer_script_smusher.rendered + user_data = templatefile("workers-configuration/pg-bouncer-instance-user-data.tpl.sh", + { + database_host = aws_db_instance.postgres_db.address + database_name = aws_db_instance.postgres_db.db_name + database_password = var.database_password + database_port = var.database_hidden_port + database_user = var.database_user + listen_port = var.database_port + region = var.region + stage = var.stage + user = var.user + } + ) tags = merge( var.default_tags, @@ -198,19 +210,3 @@ resource "aws_instance" "pg_bouncer" { tags = var.default_tags } } - -data "template_file" "pg_bouncer_script_smusher" { - template = file("workers-configuration/pg-bouncer-instance-user-data.tpl.sh") - - vars = { - database_host = aws_db_instance.postgres_db.address - database_user = var.database_user - database_port = var.database_hidden_port - database_password = var.database_password - database_name = aws_db_instance.postgres_db.name - listen_port = var.database_port - user = var.user - stage = var.stage - region = var.region - } -} diff --git a/infrastructure/instances.tf b/infrastructure/instances.tf index 379971e8d..bed2234dd 100644 --- a/infrastructure/instances.tf +++ b/infrastructure/instances.tf @@ -7,34 +7,11 @@ data "aws_ami" "ubuntu" { owners = ["589864003899"] filter { - name = "name" + name = "name" values = ["ccdl-ubuntu-18.04-*"] } } -# This script smusher exists in order to be able to circumvent a -# limitation of AWS which is that you get one script and one script -# only to set up the instance when it boots up. Because there is only -# one script you cannot place additional files your script may need -# onto the instance. Therefore this script smusher templates the files -# the instance-user-data.sh script needs into it, so that once it -# makes its way onto the instance it can spit them back out onto the -# disk. -data "template_file" "worker_script_smusher" { - template = file("workers-configuration/workers-instance-user-data.tpl.sh") - - vars = { - user = var.user - stage = var.stage - region = var.region - database_host = aws_instance.pg_bouncer.private_ip - database_port = var.database_port - database_user = var.database_user - database_password = var.database_password - database_name = aws_db_instance.postgres_db.name - } -} - ## # ElasticSearch ## @@ -53,7 +30,7 @@ data "aws_caller_identity" "current" { } resource "aws_elasticsearch_domain" "es" { - domain_name = "es-${var.user}-${var.stage}" + domain_name = "es-${var.user}-${var.stage}" elasticsearch_version = "6.3" advanced_options = { @@ -109,7 +86,7 @@ CONFIG var.default_tags, { Domain = "es-${var.user}-${var.stage}" - Name = "es-${var.user}-${var.stage}" + Name = "es-${var.user}-${var.stage}" } ) } @@ -130,56 +107,43 @@ data "local_file" "api_environment" { filename = "api-configuration/environment" } -# This script smusher serves a similar purpose to -# ${data.template_file.worker_script_smusher} but for the Nginx/API. -data "template_file" "api_server_script_smusher" { - template = file("api-configuration/api-server-instance-user-data.tpl.sh") - - vars = { - nginx_config = data.local_file.api_nginx_config.content - api_environment = data.local_file.api_environment.content - dockerhub_repo = var.dockerhub_repo - api_docker_image = var.api_docker_image - data_refinery_cert_bucket = aws_s3_bucket.data_refinery_cert_bucket.id - user = var.user - stage = var.stage - region = var.region - database_host = aws_instance.pg_bouncer.private_ip - database_user = var.database_user - database_password = var.database_password - database_name = aws_db_instance.postgres_db.name - elasticsearch_host = aws_elasticsearch_domain.es.endpoint - elasticsearch_port = "80" # AWS doesn't support the data transfer protocol on 9200 >:[ - log_group = aws_cloudwatch_log_group.data_refinery_log_group.name - log_stream = aws_cloudwatch_log_stream.log_stream_api.name - } - - depends_on = [ - aws_db_instance.postgres_db, - aws_elasticsearch_domain.es, - aws_instance.pg_bouncer, - aws_security_group_rule.data_refinery_api_http, - aws_security_group_rule.data_refinery_api_outbound, - aws_s3_bucket.data_refinery_cert_bucket, - ] -} - resource "aws_instance" "api_server_1" { - ami = data.aws_ami.ubuntu.id - instance_type = var.api_instance_type - availability_zone = "${var.region}a" + ami = data.aws_ami.ubuntu.id + instance_type = var.api_instance_type + availability_zone = "${var.region}a" vpc_security_group_ids = [aws_security_group.data_refinery_api.id] - iam_instance_profile = aws_iam_instance_profile.data_refinery_api.name - subnet_id = aws_subnet.data_refinery_1a.id + iam_instance_profile = aws_iam_instance_profile.data_refinery_api.name + subnet_id = aws_subnet.data_refinery_1a.id depends_on = [ aws_db_instance.postgres_db, aws_elasticsearch_domain.es, aws_instance.pg_bouncer, + aws_s3_bucket.data_refinery_cert_bucket, aws_security_group_rule.data_refinery_api_http, aws_security_group_rule.data_refinery_api_outbound, ] - user_data = data.template_file.api_server_script_smusher.rendered - key_name = aws_key_pair.data_refinery.key_name + + user_data = templatefile("api-configuration/api-server-instance-user-data.tpl.sh", + { + api_docker_image = var.api_docker_image + api_environment = data.local_file.api_environment.content + data_refinery_cert_bucket = aws_s3_bucket.data_refinery_cert_bucket.id + database_host = aws_instance.pg_bouncer.private_ip + database_name = aws_db_instance.postgres_db.db_name + database_password = var.database_password + database_user = var.database_user + dockerhub_repo = var.dockerhub_repo + elasticsearch_host = aws_elasticsearch_domain.es.endpoint + elasticsearch_port = "80" # AWS doesn't support the data transfer protocol on 9200 >:[ + log_group = aws_cloudwatch_log_group.data_refinery_log_group.name + log_stream = aws_cloudwatch_log_stream.log_stream_api.name + nginx_config = data.local_file.api_nginx_config.content + region = var.region + stage = var.stage + user = var.user + } + ) + key_name = aws_key_pair.data_refinery.key_name tags = merge( var.default_tags, @@ -210,44 +174,44 @@ data "local_file" "foreman_environment" { filename = "foreman-configuration/environment" } -# This script smusher serves a similar purpose to -# ${data.template_file.worker_script_smusher} but for the Foreman. -data "template_file" "foreman_server_script_smusher" { - template = file( - "foreman-configuration/foreman-server-instance-user-data.tpl.sh", - ) - - vars = { - foreman_environment = data.local_file.foreman_environment.content - dockerhub_repo = var.dockerhub_repo - foreman_docker_image = var.foreman_docker_image - user = var.user - stage = var.stage - region = var.region - database_host = aws_instance.pg_bouncer.private_ip - database_user = var.database_user - database_password = var.database_password - database_name = aws_db_instance.postgres_db.name - elasticsearch_host = aws_elasticsearch_domain.es.endpoint - elasticsearch_port = var.elasticsearch_port - log_group = aws_cloudwatch_log_group.data_refinery_log_group.name - } -} - resource "aws_instance" "foreman_server_1" { - ami = data.aws_ami.ubuntu.id - instance_type = var.foreman_instance_type - availability_zone = "${var.region}a" + ami = data.aws_ami.ubuntu.id + instance_type = var.foreman_instance_type + availability_zone = "${var.region}a" vpc_security_group_ids = [aws_security_group.data_refinery_foreman.id] - iam_instance_profile = aws_iam_instance_profile.data_refinery_foreman.name - subnet_id = aws_subnet.data_refinery_1a.id + iam_instance_profile = aws_iam_instance_profile.data_refinery_foreman.name + subnet_id = aws_subnet.data_refinery_1a.id + depends_on = [ aws_db_instance.postgres_db, - aws_instance.pg_bouncer, aws_elasticsearch_domain.es, + aws_instance.pg_bouncer, + aws_s3_bucket.data_refinery_cert_bucket, + aws_security_group_rule.data_refinery_api_http, + aws_security_group_rule.data_refinery_api_outbound, ] - user_data = data.template_file.foreman_server_script_smusher.rendered - key_name = aws_key_pair.data_refinery.key_name + + user_data = templatefile("api-configuration/api-server-instance-user-data.tpl.sh", + { + api_docker_image = var.api_docker_image + api_environment = data.local_file.api_environment.content + data_refinery_cert_bucket = aws_s3_bucket.data_refinery_cert_bucket.id + database_host = aws_instance.pg_bouncer.private_ip + database_name = aws_db_instance.postgres_db.db_name + database_password = var.database_password + database_user = var.database_user + dockerhub_repo = var.dockerhub_repo + elasticsearch_host = aws_elasticsearch_domain.es.endpoint + elasticsearch_port = "80" # AWS doesn't support the data transfer protocol on 9200 >:[ + log_group = aws_cloudwatch_log_group.data_refinery_log_group.name + log_stream = aws_cloudwatch_log_stream.log_stream_api.name + nginx_config = data.local_file.api_nginx_config.content + region = var.region + stage = var.stage + user = var.user + } + ) + key_name = aws_key_pair.data_refinery.key_name tags = merge( var.default_tags, diff --git a/infrastructure/variables.tf b/infrastructure/variables.tf index 3d56947a0..ab2589f14 100644 --- a/infrastructure/variables.tf +++ b/infrastructure/variables.tf @@ -256,7 +256,7 @@ output "environment_variables" { }, { name = "DATABASE_NAME" - value = aws_db_instance.postgres_db.name + value = aws_db_instance.postgres_db.db_name }, { name = "DATABASE_HOST" From 96867782b7ce3d38336eb3d78af18357d726741f Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 6 Oct 2022 09:19:40 -0700 Subject: [PATCH 027/220] Fix foreman misconfiguration. --- .../foreman-server-instance-user-data.tpl.sh | 9 +++--- infrastructure/instances.tf | 32 +++++++++---------- infrastructure/variables.tf | 8 +++++ 3 files changed, 28 insertions(+), 21 deletions(-) diff --git a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh index e77541c75..3598c2a45 100644 --- a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh +++ b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh @@ -77,8 +77,8 @@ docker run \\ -e DATABASE_PASSWORD=${database_password} \\ -v /tmp:/tmp \\ -it ${dockerhub_repo}/dr_\"\$1\" python3 manage.py \"\$2\" -" >> /home/ubuntu/run_cron_job_test.sh -chmod +x /home/ubuntu/run_cron_job_test.sh +" >> /home/ubuntu/run_cron_job.sh +chmod +x /home/ubuntu/run_cron_job.sh # Use Monit to ensure the Foreman is always running apt-get -y update @@ -112,8 +112,9 @@ service monit restart # Install the cron job tests crontab -l > tempcron cat <> tempcron -0 12 * * MON /bin/bash /home/ubuntu/run_cron_job_test.sh affymetrix check_brainarray_gene_agreement >> /var/log/cron_job_tests.log 2>&1 -0 12 * * MON /bin/bash /home/ubuntu/run_cron_job_test.sh affymetrix check_tx_index_transcript_agreement >> /var/log/cron_job_tests.log 2>&1 +0 12 * * MON /bin/bash /home/ubuntu/run_cron_job.sh affymetrix check_brainarray_gene_agreement >> /var/log/cron_job_tests.log 2>&1 +0 12 * * MON /bin/bash /home/ubuntu/run_cron_job.sh affymetrix check_tx_index_transcript_agreement >> /var/log/cron_job_tests.log 2>&1 +0 12 * * ${accession_gathering_job_run_day} /bin/bash /home/ubuntu/run_cron_job.sh foreman gather_weekly_accessions >> /var/log/gather_weekly_accessions.log 2>&1 EOF # install new cron file crontab tempcron diff --git a/infrastructure/instances.tf b/infrastructure/instances.tf index bed2234dd..0628c31d3 100644 --- a/infrastructure/instances.tf +++ b/infrastructure/instances.tf @@ -191,24 +191,22 @@ resource "aws_instance" "foreman_server_1" { aws_security_group_rule.data_refinery_api_outbound, ] - user_data = templatefile("api-configuration/api-server-instance-user-data.tpl.sh", + user_data = templatefile("foreman-configuration/foreman-server-instance-user-data.tpl.sh", { - api_docker_image = var.api_docker_image - api_environment = data.local_file.api_environment.content - data_refinery_cert_bucket = aws_s3_bucket.data_refinery_cert_bucket.id - database_host = aws_instance.pg_bouncer.private_ip - database_name = aws_db_instance.postgres_db.db_name - database_password = var.database_password - database_user = var.database_user - dockerhub_repo = var.dockerhub_repo - elasticsearch_host = aws_elasticsearch_domain.es.endpoint - elasticsearch_port = "80" # AWS doesn't support the data transfer protocol on 9200 >:[ - log_group = aws_cloudwatch_log_group.data_refinery_log_group.name - log_stream = aws_cloudwatch_log_stream.log_stream_api.name - nginx_config = data.local_file.api_nginx_config.content - region = var.region - stage = var.stage - user = var.user + accession_gathering_job_run_day = var.accession_gathering_job_run_day + database_host = aws_instance.pg_bouncer.private_ip + database_name = aws_db_instance.postgres_db.name + database_password = var.database_password + database_user = var.database_user + dockerhub_repo = var.dockerhub_repo + elasticsearch_host = aws_elasticsearch_domain.es.endpoint + elasticsearch_port = var.elasticsearch_port + foreman_docker_image = var.foreman_docker_image + foreman_environment = data.local_file.foreman_environment.content + log_group = aws_cloudwatch_log_group.data_refinery_log_group.name + region = var.region + stage = var.stage + user = var.user } ) key_name = aws_key_pair.data_refinery.key_name diff --git a/infrastructure/variables.tf b/infrastructure/variables.tf index ab2589f14..8bf167971 100644 --- a/infrastructure/variables.tf +++ b/infrastructure/variables.tf @@ -223,6 +223,14 @@ variable "processing_compendia" { default = true } +variable "accession_gathering_job_run_day" { + default = "SAT" +} + +variable "max_accessions_gathered_per_run" { + default = 0 +} + # Output our production environment variables. output "environment_variables" { value = [ From 8b79baecaf8b489add70d04c48fbc967fed626e5 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 6 Oct 2022 14:27:20 -0700 Subject: [PATCH 028/220] Update AMI terraform config. --- ami/instance.tf | 16 +++++++++++----- ami/user-data-script.tf | 16 ---------------- 2 files changed, 11 insertions(+), 21 deletions(-) diff --git a/ami/instance.tf b/ami/instance.tf index 620b13803..41c01ae48 100644 --- a/ami/instance.tf +++ b/ami/instance.tf @@ -1,5 +1,4 @@ provider "aws" { - version = "2.70.0" region = "us-east-1" } @@ -26,10 +25,14 @@ resource "aws_instance" "ubuntu-ami-template-instance" { # Our instance-user-data.sh script is built by Terraform at # apply-time so that it can put additional files onto the # instance. For more information see the definition of this resource. - user_data = data.template_file.ubuntu_instance_user_data.rendered + user_data = templatefile("ubuntu-instance-user-data.tpl.sh", { + docker_apt_key = data.local_file.docker_apt_key.content + } + ) - subnet_id = data.aws_subnet.ccdl_dev_subnet.id associate_public_ip_address = true + key_name = "data-refinery-key-circleci-prod" + subnet_id = data.aws_subnet.ccdl_dev_subnet.id vpc_security_group_ids = [aws_security_group.ami_template_instance.id] tags = { @@ -46,11 +49,14 @@ resource "aws_instance" "ecs-ami-template-instance" { # Our instance-user-data.sh script is built by Terraform at # apply-time so that it can put additional files onto the # instance. For more information see the definition of this resource. - user_data = data.template_file.ecs_instance_user_data.rendered + user_data = templatefile("ecs-instance-user-data.tpl.sh", { + docker_apt_key = data.local_file.docker_apt_key.content + } + ) - subnet_id = data.aws_subnet.ccdl_dev_subnet.id associate_public_ip_address = true key_name = "data-refinery-key-circleci-prod" + subnet_id = data.aws_subnet.ccdl_dev_subnet.id vpc_security_group_ids = [aws_security_group.ami_template_instance.id] tags = { diff --git a/ami/user-data-script.tf b/ami/user-data-script.tf index d5d0718e3..585beacbe 100644 --- a/ami/user-data-script.tf +++ b/ami/user-data-script.tf @@ -2,19 +2,3 @@ data "local_file" "docker_apt_key" { filename = "docker-apt-key.gpg" } - -data "template_file" "ubuntu_instance_user_data" { - template = file("ubuntu-instance-user-data.tpl.sh") - - vars = { - docker_apt_key = data.local_file.docker_apt_key.content - } -} - -data "template_file" "ecs_instance_user_data" { - template = file("ecs-instance-user-data.tpl.sh") - - vars = { - docker_apt_key = data.local_file.docker_apt_key.content - } -} From 7215e1244ed485cbb32c15ee452388d2df175c87 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 6 Oct 2022 17:50:56 -0700 Subject: [PATCH 029/220] Update .gitignore: - exclude terraform files - exclude VSCode project files - exclude `errors.txt` file --- .gitignore | 11 ++++++++--- 1 file changed, 8 insertions(+), 3 deletions(-) diff --git a/.gitignore b/.gitignore index 55b0a1f8e..1a543b4b8 100644 --- a/.gitignore +++ b/.gitignore @@ -17,11 +17,12 @@ foreman/batch-job-specs/* !foreman/batch-job-specs/*.tpl # Terraform -infrastructure/.terraform/ -ami/.terraform/ -.circleci/s3_tfstate/.terraform +.terraform +.terraform.lock.hcl infrastructure/api-configuration/environment infrastructure/foreman-configuration/environment +terraform.tfstate +terraform.tfstate.backup # Files that are created during deploys infrastructure/batch-job-templates/ @@ -141,6 +142,7 @@ dr_env/ #VS Code .vscode +*.code-workspace # OSX .DS_Store @@ -154,3 +156,6 @@ dr_env/ # vim swp files *.*.swp + +# Other +errors.txt From 16fa51e0a9177c9240e910c3cd0ce1582d6f783c Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 19 Oct 2022 11:23:40 -0700 Subject: [PATCH 030/220] Refactor `script_directory` setting code. --- api/run_tests.sh | 4 +--- api/serve.sh | 4 +--- api/serve_production.sh | 4 +--- common/run_tests.sh | 4 +--- config/externally_supplied_metadata/metasra/translate.sh | 4 +--- foreman/run_end_to_end_tests.sh | 4 +--- foreman/run_management_command.sh | 4 +--- foreman/run_tests.sh | 4 +--- foreman/test_survey.sh | 4 +--- infrastructure/deploy.sh | 4 +--- infrastructure/destroy_terraform.sh | 4 +--- scripts/create_virtualenv.sh | 4 +--- scripts/format_batch_with_env.sh | 4 +--- scripts/install_all.sh | 4 +--- scripts/kill_all_jobs.sh | 4 +--- scripts/make_migrations.sh | 4 +--- scripts/prepare_image.sh | 4 +--- scripts/rebuild_es_index.sh | 4 +--- scripts/reinit_database.sh | 4 +--- scripts/run_all_tests.sh | 4 +--- scripts/run_manage.sh | 4 +--- scripts/run_postgres.sh | 4 +--- scripts/run_shell.sh | 4 +--- scripts/update_models.sh | 4 +--- scripts/update_my_docker_images.sh | 4 +--- workers/run_command.sh | 4 +--- workers/run_janitor.sh | 4 +--- workers/run_job.sh | 4 +--- workers/run_tests.sh | 4 +--- 29 files changed, 29 insertions(+), 87 deletions(-) diff --git a/api/run_tests.sh b/api/run_tests.sh index 3bf778973..aea19826d 100755 --- a/api/run_tests.sh +++ b/api/run_tests.sh @@ -4,9 +4,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to diff --git a/api/serve.sh b/api/serve.sh index f1246e7a9..4bae59fe9 100755 --- a/api/serve.sh +++ b/api/serve.sh @@ -4,9 +4,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to diff --git a/api/serve_production.sh b/api/serve_production.sh index 9962b337d..530b237ca 100755 --- a/api/serve_production.sh +++ b/api/serve_production.sh @@ -4,9 +4,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to diff --git a/common/run_tests.sh b/common/run_tests.sh index fa5fd1bd1..06de9ac44 100755 --- a/common/run_tests.sh +++ b/common/run_tests.sh @@ -4,9 +4,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to diff --git a/config/externally_supplied_metadata/metasra/translate.sh b/config/externally_supplied_metadata/metasra/translate.sh index 9091d9d6b..48d0f4305 100755 --- a/config/externally_supplied_metadata/metasra/translate.sh +++ b/config/externally_supplied_metadata/metasra/translate.sh @@ -11,9 +11,7 @@ PROCESSED_ACCESSIONS_FILE="$(basename -s .tab "$ACCESSIONS_FILE").processed.tab" # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit if ! [ -e "$JSON_FILE" ]; then diff --git a/foreman/run_end_to_end_tests.sh b/foreman/run_end_to_end_tests.sh index 1199b9a19..03e3fd4b8 100755 --- a/foreman/run_end_to_end_tests.sh +++ b/foreman/run_end_to_end_tests.sh @@ -4,9 +4,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Set up the data volume directory if it does not already exist. diff --git a/foreman/run_management_command.sh b/foreman/run_management_command.sh index 9ad3bd0f0..08231665a 100755 --- a/foreman/run_management_command.sh +++ b/foreman/run_management_command.sh @@ -4,9 +4,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to diff --git a/foreman/run_tests.sh b/foreman/run_tests.sh index f4c594763..a78a128ab 100755 --- a/foreman/run_tests.sh +++ b/foreman/run_tests.sh @@ -4,9 +4,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Set up the data volume directory if it does not already exist. diff --git a/foreman/test_survey.sh b/foreman/test_survey.sh index 55e662174..7a4016573 100755 --- a/foreman/test_survey.sh +++ b/foreman/test_survey.sh @@ -48,9 +48,7 @@ fi # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Set up the data volume directory if it does not already exist. diff --git a/infrastructure/deploy.sh b/infrastructure/deploy.sh index 47dcab5c6..d8175b423 100755 --- a/infrastructure/deploy.sh +++ b/infrastructure/deploy.sh @@ -2,9 +2,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit print_description() { diff --git a/infrastructure/destroy_terraform.sh b/infrastructure/destroy_terraform.sh index 6a784963b..cf91053e4 100755 --- a/infrastructure/destroy_terraform.sh +++ b/infrastructure/destroy_terraform.sh @@ -2,9 +2,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit print_description() { diff --git a/scripts/create_virtualenv.sh b/scripts/create_virtualenv.sh index 7b1cf4f9a..5857f3f40 100755 --- a/scripts/create_virtualenv.sh +++ b/scripts/create_virtualenv.sh @@ -2,9 +2,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give virtualenv access to all the code we have to diff --git a/scripts/format_batch_with_env.sh b/scripts/format_batch_with_env.sh index 5ab5bd386..a86ee36b5 100755 --- a/scripts/format_batch_with_env.sh +++ b/scripts/format_batch_with_env.sh @@ -105,9 +105,7 @@ fi # This script should always run from the context of the directory of # the project it is building. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" project_directory="$script_directory/.." diff --git a/scripts/install_all.sh b/scripts/install_all.sh index 22a44cc89..0d389712e 100755 --- a/scripts/install_all.sh +++ b/scripts/install_all.sh @@ -8,9 +8,7 @@ TERRAFORM_VERSION="0.13.5" # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit print_description() { diff --git a/scripts/kill_all_jobs.sh b/scripts/kill_all_jobs.sh index e57b3e510..6ef70be94 100755 --- a/scripts/kill_all_jobs.sh +++ b/scripts/kill_all_jobs.sh @@ -2,9 +2,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit while read -r row; do diff --git a/scripts/make_migrations.sh b/scripts/make_migrations.sh index 689799e43..25ef9e6c8 100755 --- a/scripts/make_migrations.sh +++ b/scripts/make_migrations.sh @@ -8,9 +8,7 @@ set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit ./prepare_image.sh -i migrations -s common diff --git a/scripts/prepare_image.sh b/scripts/prepare_image.sh index c1d809367..e791ce589 100755 --- a/scripts/prepare_image.sh +++ b/scripts/prepare_image.sh @@ -2,9 +2,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Import the functions in common.sh diff --git a/scripts/rebuild_es_index.sh b/scripts/rebuild_es_index.sh index 17ebe8462..30fded027 100755 --- a/scripts/rebuild_es_index.sh +++ b/scripts/rebuild_es_index.sh @@ -2,9 +2,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit ./run_manage.sh -i api_local -s api search_index --rebuild -f diff --git a/scripts/reinit_database.sh b/scripts/reinit_database.sh index 1f4b01385..18107000d 100755 --- a/scripts/reinit_database.sh +++ b/scripts/reinit_database.sh @@ -5,9 +5,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Clear it out. diff --git a/scripts/run_all_tests.sh b/scripts/run_all_tests.sh index 0d2f68985..9372e43f2 100755 --- a/scripts/run_all_tests.sh +++ b/scripts/run_all_tests.sh @@ -7,9 +7,7 @@ set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Get access to all of the refinebio project diff --git a/scripts/run_manage.sh b/scripts/run_manage.sh index 1a9417d59..9ccb4761d 100755 --- a/scripts/run_manage.sh +++ b/scripts/run_manage.sh @@ -6,9 +6,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Import the functions in common.sh diff --git a/scripts/run_postgres.sh b/scripts/run_postgres.sh index 15d43a5dd..2798dc04b 100755 --- a/scripts/run_postgres.sh +++ b/scripts/run_postgres.sh @@ -2,9 +2,7 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Get access to all of refinebio diff --git a/scripts/run_shell.sh b/scripts/run_shell.sh index bb6a2b350..d5be913ad 100755 --- a/scripts/run_shell.sh +++ b/scripts/run_shell.sh @@ -12,9 +12,7 @@ set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Import functions in common.sh diff --git a/scripts/update_models.sh b/scripts/update_models.sh index c5ffe72cc..d6648df8f 100755 --- a/scripts/update_models.sh +++ b/scripts/update_models.sh @@ -6,9 +6,7 @@ set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Get access to all of refinebio diff --git a/scripts/update_my_docker_images.sh b/scripts/update_my_docker_images.sh index 025e9ad1f..e49d1d542 100755 --- a/scripts/update_my_docker_images.sh +++ b/scripts/update_my_docker_images.sh @@ -8,9 +8,7 @@ set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # Get access to all of refinebio diff --git a/workers/run_command.sh b/workers/run_command.sh index 7ada45b5e..0edfd614f 100755 --- a/workers/run_command.sh +++ b/workers/run_command.sh @@ -26,9 +26,7 @@ fi # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to diff --git a/workers/run_janitor.sh b/workers/run_janitor.sh index e8fbcd9bf..27c6ed6f5 100755 --- a/workers/run_janitor.sh +++ b/workers/run_janitor.sh @@ -1,9 +1,7 @@ #!/bin/sh # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to diff --git a/workers/run_job.sh b/workers/run_job.sh index 779fa5844..081b94646 100755 --- a/workers/run_job.sh +++ b/workers/run_job.sh @@ -61,9 +61,7 @@ fi # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to diff --git a/workers/run_tests.sh b/workers/run_tests.sh index 32f8c7e08..a750b502a 100755 --- a/workers/run_tests.sh +++ b/workers/run_tests.sh @@ -44,9 +44,7 @@ done # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(perl -e 'use File::Basename; - use Cwd "abs_path"; - print dirname(abs_path(@ARGV[0]));' -- "$0")" +script_directory="$(cd "$(dirname "$0")" || exit; pwd)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to From 042fa9e2ce3deade5ec3d6ea49424d5bd247e294 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 21 Oct 2022 18:11:07 -0700 Subject: [PATCH 031/220] Move all tests to `tests` directory. Extract testing env settings into a separate file (tests/settings.py). Add setup.cfg to the Docker container for coverate.report settings. Update `test_all_endpoints` test to use current job IDs instead of hardcoded '1'. --- .pre-commit-config.yaml | 2 +- api/data_refinery_api/middleware.py | 10 +++- api/data_refinery_api/settings.py | 29 +++++---- api/data_refinery_api/test_imports.py | 16 ----- api/data_refinery_api/urls.py | 14 ++++- api/data_refinery_api/views/api_token.py | 4 +- api/data_refinery_api/views/dataset.py | 60 ++++++++++++++----- api/data_refinery_api/views/experiment.py | 6 +- .../views/experiment_document.py | 5 +- .../views/jobs/downloader_job.py | 2 +- .../views/jobs/processor_job.py | 2 +- .../views/jobs/survey_job.py | 2 +- api/data_refinery_api/views/stats.py | 4 +- api/dockerfiles/Dockerfile.api_local | 1 + .../test => tests}/__init__.py | 0 api/tests/management/__init__.py | 0 api/tests/management/commands/__init__.py | 0 .../commands/test_post_downloads_summary.py | 0 api/tests/settings.py | 10 ++++ api/tests/test_imports.py | 16 +++++ .../test => tests}/test_sentry_middleware.py | 5 +- api/tests/views/__init__.py | 0 .../test => tests/views}/test_api_general.py | 59 ++++++++++-------- .../test => tests/views}/test_compendia.py | 9 ++- .../test => tests/views}/test_dataset.py | 22 +++++-- .../views}/test_dataset_stats.py | 6 +- .../test => tests/views}/test_processor.py | 3 +- .../test => tests/views}/test_qn_target.py | 3 +- .../test => tests/views}/test_search.py | 9 ++- .../test => tests/views}/test_stats.py | 3 +- scripts/common.sh | 2 +- setup.cfg | 28 +++++---- 32 files changed, 219 insertions(+), 113 deletions(-) delete mode 100644 api/data_refinery_api/test_imports.py rename api/{data_refinery_api/test => tests}/__init__.py (100%) create mode 100644 api/tests/management/__init__.py create mode 100644 api/tests/management/commands/__init__.py rename api/{data_refinery_api => tests}/management/commands/test_post_downloads_summary.py (100%) create mode 100644 api/tests/settings.py create mode 100644 api/tests/test_imports.py rename api/{data_refinery_api/test => tests}/test_sentry_middleware.py (96%) create mode 100644 api/tests/views/__init__.py rename api/{data_refinery_api/test => tests/views}/test_api_general.py (93%) rename api/{data_refinery_api/test => tests/views}/test_compendia.py (97%) rename api/{data_refinery_api/test => tests/views}/test_dataset.py (97%) rename api/{data_refinery_api/test => tests/views}/test_dataset_stats.py (96%) rename api/{data_refinery_api/test => tests/views}/test_processor.py (98%) rename api/{data_refinery_api/test => tests/views}/test_qn_target.py (96%) rename api/{data_refinery_api/test => tests/views}/test_search.py (97%) rename api/{data_refinery_api/test => tests/views}/test_stats.py (98%) diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index d024704da..b651ce24a 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -22,7 +22,7 @@ repos: - id: isort - repo: https://github.com/psf/black - rev: 19.10b0 + rev: 22.3.0 hooks: - id: black args: [--line-length=100] diff --git a/api/data_refinery_api/middleware.py b/api/data_refinery_api/middleware.py index a9bd5c503..988dfae59 100644 --- a/api/data_refinery_api/middleware.py +++ b/api/data_refinery_api/middleware.py @@ -25,7 +25,10 @@ def process_response(self, request, response): # create data representation data = client.get_data_from_request(request) data.update( - {"level": logging.WARN, "logger": "BadRequestMiddleware",} + { + "level": logging.WARN, + "logger": "BadRequestMiddleware", + } ) client.captureMessage(message=message, data=data) @@ -40,7 +43,10 @@ def process_exception(self, request, exception): data = client.get_data_from_request(request) data.update( - {"level": logging.WARN, "logger": "BadRequestMiddleware",} + { + "level": logging.WARN, + "logger": "BadRequestMiddleware", + } ) client.captureMessage(message=str(exception), data=data) diff --git a/api/data_refinery_api/settings.py b/api/data_refinery_api/settings.py index 7d2ea00f5..fa3cd2228 100644 --- a/api/data_refinery_api/settings.py +++ b/api/data_refinery_api/settings.py @@ -121,10 +121,18 @@ # https://docs.djangoproject.com/en/1.10/ref/settings/#auth-password-validators AUTH_PASSWORD_VALIDATORS = [ - {"NAME": ("django.contrib.auth.password_validation" + ".UserAttributeSimilarityValidator"),}, - {"NAME": ("django.contrib.auth.password_validation." + "MinimumLengthValidator"),}, - {"NAME": ("django.contrib.auth.password_validation" + ".CommonPasswordValidator"),}, - {"NAME": ("django.contrib.auth.password_validation" + ".NumericPasswordValidator"),}, + { + "NAME": ("django.contrib.auth.password_validation.UserAttributeSimilarityValidator"), + }, + { + "NAME": ("django.contrib.auth.password_validation.MinimumLengthValidator"), + }, + { + "NAME": ("django.contrib.auth.password_validation.CommonPasswordValidator"), + }, + { + "NAME": ("django.contrib.auth.password_validation.NumericPasswordValidator"), + }, ] @@ -228,15 +236,10 @@ } } -if "test" in sys.argv: - ELASTICSEARCH_INDEX_NAMES = { - "data_refinery_common.models.documents": "experiments_test", - } -else: - ELASTICSEARCH_INDEX_NAMES = { - "data_refinery_common.models.documents": "experiments", - } - ELASTICSEARCH_DSL_AUTOSYNC = False +ELASTICSEARCH_INDEX_NAMES = { + "data_refinery_common.models.documents": "experiments", +} +ELASTICSEARCH_DSL_AUTOSYNC = False # ToS TERMS_AND_CONDITIONS = """Welcome to the Alex’s Lemonade Childhood Cancer Data Lab, which is supported by Alex’s Lemonade Stand Foundation ("we," "our," or "us"). These Terms of Use (these "Terms") are a binding legal agreement between you and us regarding your access to and use of the websites located at https://www.ccdatalab.org, https://cognoma.org, http://www.refine.bio or any subdomains thereof and any embedded or associated software, applications, data or other content, provided or managed by us in connection with such websites (collectively, as may be updated, modified or replaced from time to time, the "CCDL"). diff --git a/api/data_refinery_api/test_imports.py b/api/data_refinery_api/test_imports.py deleted file mode 100644 index 2d4d8b2dd..000000000 --- a/api/data_refinery_api/test_imports.py +++ /dev/null @@ -1,16 +0,0 @@ -from django.test import TestCase, tag - - -class ImportTestCase(TestCase): - def test_imports(self): - # Make sure we can import the api tests - import data_refinery_api.management.commands.test_post_downloads_summary - import data_refinery_api.test.test_api_general - import data_refinery_api.test.test_compendia - import data_refinery_api.test.test_dataset - import data_refinery_api.test.test_dataset_stats - import data_refinery_api.test.test_processor - import data_refinery_api.test.test_qn_target - import data_refinery_api.test.test_search - import data_refinery_api.test.test_sentry_middleware - import data_refinery_api.test.test_stats diff --git a/api/data_refinery_api/urls.py b/api/data_refinery_api/urls.py index ff30ee2c6..751e85929 100644 --- a/api/data_refinery_api/urls.py +++ b/api/data_refinery_api/urls.py @@ -59,12 +59,22 @@ def handle404error(request, exception): if not match(r"^/v[1-9]/.*", request.path): message = "refine.bio API resources are only available through versioned requests." - return JsonResponse({"message": message, "docs": url, "status_code": 404,}, status=404) + return JsonResponse( + { + "message": message, + "docs": url, + "status_code": 404, + }, + status=404, + ) def handle500error(request): return JsonResponse( - {"message": "A server error occured. This has been reported.", "status_code": 500,}, + { + "message": "A server error occured. This has been reported.", + "status_code": 500, + }, status=500, ) diff --git a/api/data_refinery_api/views/api_token.py b/api/data_refinery_api/views/api_token.py index 34e73aef9..71da7aabc 100644 --- a/api/data_refinery_api/views/api_token.py +++ b/api/data_refinery_api/views/api_token.py @@ -49,7 +49,9 @@ class Meta: ) @method_decorator( name="retrieve", - decorator=swagger_auto_schema(operation_description="Return details about a specific token.",), + decorator=swagger_auto_schema( + operation_description="Return details about a specific token.", + ), ) @method_decorator( name="update", diff --git a/api/data_refinery_api/views/dataset.py b/api/data_refinery_api/views/dataset.py index cf65489f4..c3699101c 100644 --- a/api/data_refinery_api/views/dataset.py +++ b/api/data_refinery_api/views/dataset.py @@ -221,24 +221,54 @@ class Meta: "worker_version", ) extra_kwargs = { - "data": {"required": True,}, - "id": {"read_only": True,}, - "is_processing": {"read_only": True,}, - "is_processed": {"read_only": True,}, - "is_available": {"read_only": True,}, + "data": { + "required": True, + }, + "id": { + "read_only": True, + }, + "is_processing": { + "read_only": True, + }, + "is_processed": { + "read_only": True, + }, + "is_available": { + "read_only": True, + }, "email_address": {"required": False, "write_only": True}, "email_ccdl_ok": {"required": False, "write_only": True}, "notify_me": {"required": False, "write_only": True}, - "expires_on": {"read_only": True,}, - "s3_bucket": {"read_only": True,}, - "s3_key": {"read_only": True,}, - "success": {"read_only": True,}, - "failure_reason": {"read_only": True,}, - "created_at": {"read_only": True,}, - "last_modified": {"read_only": True,}, - "size_in_bytes": {"read_only": True,}, - "sha1": {"read_only": True,}, - "download_url": {"read_only": True,}, + "expires_on": { + "read_only": True, + }, + "s3_bucket": { + "read_only": True, + }, + "s3_key": { + "read_only": True, + }, + "success": { + "read_only": True, + }, + "failure_reason": { + "read_only": True, + }, + "created_at": { + "read_only": True, + }, + "last_modified": { + "read_only": True, + }, + "size_in_bytes": { + "read_only": True, + }, + "sha1": { + "read_only": True, + }, + "download_url": { + "read_only": True, + }, "worker_version": { "read_only": True, "help_text": "Returns the latest version of refine.bio that was used to build this dataset.", diff --git a/api/data_refinery_api/views/experiment.py b/api/data_refinery_api/views/experiment.py index d7148d509..6ed81f2e8 100644 --- a/api/data_refinery_api/views/experiment.py +++ b/api/data_refinery_api/views/experiment.py @@ -66,7 +66,7 @@ class Meta: @staticmethod def setup_eager_loading(queryset): - """ Perform necessary eager loading of data. """ + """Perform necessary eager loading of data.""" queryset = queryset.prefetch_related("samples").prefetch_related("organisms") # Multiple count annotations @@ -117,7 +117,7 @@ class Meta: class ExperimentListView(generics.ListAPIView): - """ Paginated list of all experiments. Advanced filtering can be done with the `/search` endpoint. """ + """Paginated list of all experiments. Advanced filtering can be done with the `/search` endpoint.""" model = Experiment queryset = Experiment.public_objects.all() @@ -152,7 +152,7 @@ def get_queryset(self): class ExperimentDetailView(generics.RetrieveAPIView): - """ Retrieve details for an experiment given it's accession code """ + """Retrieve details for an experiment given it's accession code""" lookup_field = "accession_code" queryset = Experiment.public_objects.all() diff --git a/api/data_refinery_api/views/experiment_document.py b/api/data_refinery_api/views/experiment_document.py index e1780c791..b1e177a56 100644 --- a/api/data_refinery_api/views/experiment_document.py +++ b/api/data_refinery_api/views/experiment_document.py @@ -276,7 +276,10 @@ class ExperimentDocumentView(DocumentViewSet): # Define filtering fields filter_fields = { - "id": {"field": "_id", "lookups": [LOOKUP_FILTER_RANGE, LOOKUP_QUERY_IN],}, + "id": { + "field": "_id", + "lookups": [LOOKUP_FILTER_RANGE, LOOKUP_QUERY_IN], + }, "technology": "technology", "has_publication": "has_publication", "accession_code": "accession_code", diff --git a/api/data_refinery_api/views/jobs/downloader_job.py b/api/data_refinery_api/views/jobs/downloader_job.py index 730de72e5..33419b54d 100644 --- a/api/data_refinery_api/views/jobs/downloader_job.py +++ b/api/data_refinery_api/views/jobs/downloader_job.py @@ -120,7 +120,7 @@ def get_queryset(self): class DownloaderJobDetailView(generics.RetrieveAPIView): - """ Retrieves a DownloaderJob by ID """ + """Retrieves a DownloaderJob by ID""" lookup_field = "id" model = DownloaderJob diff --git a/api/data_refinery_api/views/jobs/processor_job.py b/api/data_refinery_api/views/jobs/processor_job.py index a7e590e5a..1d86f9cf0 100644 --- a/api/data_refinery_api/views/jobs/processor_job.py +++ b/api/data_refinery_api/views/jobs/processor_job.py @@ -121,7 +121,7 @@ def get_queryset(self): class ProcessorJobDetailView(generics.RetrieveAPIView): - """ Retrieves a ProcessorJob by ID """ + """Retrieves a ProcessorJob by ID""" lookup_field = "id" model = ProcessorJob diff --git a/api/data_refinery_api/views/jobs/survey_job.py b/api/data_refinery_api/views/jobs/survey_job.py index 80d04afdd..b3f492932 100644 --- a/api/data_refinery_api/views/jobs/survey_job.py +++ b/api/data_refinery_api/views/jobs/survey_job.py @@ -90,7 +90,7 @@ def get_queryset(self): class SurveyJobDetailView(generics.RetrieveAPIView): - """ Retrieves a SurveyJob by ID """ + """Retrieves a SurveyJob by ID""" lookup_field = "id" model = SurveyJob diff --git a/api/data_refinery_api/views/stats.py b/api/data_refinery_api/views/stats.py index 19ac6360a..25c7c3fdf 100644 --- a/api/data_refinery_api/views/stats.py +++ b/api/data_refinery_api/views/stats.py @@ -116,7 +116,9 @@ def get_jobs_in_queue(batch_job_queue: str) -> list: while "nextToken" in list_jobs_dict and list_jobs_dict["nextToken"]: list_jobs_dict = batch.list_jobs( - jobQueue=batch_job_queue, jobStatus=status, nextToken=list_jobs_dict["nextToken"], + jobQueue=batch_job_queue, + jobStatus=status, + nextToken=list_jobs_dict["nextToken"], ) jobs.extend(list_jobs_dict["jobSummaryList"]) diff --git a/api/dockerfiles/Dockerfile.api_local b/api/dockerfiles/Dockerfile.api_local index f52c29d4f..5ad7c771f 100644 --- a/api/dockerfiles/Dockerfile.api_local +++ b/api/dockerfiles/Dockerfile.api_local @@ -22,6 +22,7 @@ RUN pip install \ common/$(ls common -1 | sort --version-sort | tail -1) COPY api/ . +COPY setup.cfg . ARG SYSTEM_VERSION diff --git a/api/data_refinery_api/test/__init__.py b/api/tests/__init__.py similarity index 100% rename from api/data_refinery_api/test/__init__.py rename to api/tests/__init__.py diff --git a/api/tests/management/__init__.py b/api/tests/management/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/api/tests/management/commands/__init__.py b/api/tests/management/commands/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/api/data_refinery_api/management/commands/test_post_downloads_summary.py b/api/tests/management/commands/test_post_downloads_summary.py similarity index 100% rename from api/data_refinery_api/management/commands/test_post_downloads_summary.py rename to api/tests/management/commands/test_post_downloads_summary.py diff --git a/api/tests/settings.py b/api/tests/settings.py new file mode 100644 index 000000000..66f43b2c7 --- /dev/null +++ b/api/tests/settings.py @@ -0,0 +1,10 @@ +""" +Django settings for data_refinery_api project tests. +""" + +from data_refinery_api.settings import * + +ELASTICSEARCH_INDEX_NAMES = { + "data_refinery_common.models.documents": "experiments_test", +} +ELASTICSEARCH_DSL_AUTOSYNC = True diff --git a/api/tests/test_imports.py b/api/tests/test_imports.py new file mode 100644 index 000000000..c7048c3b3 --- /dev/null +++ b/api/tests/test_imports.py @@ -0,0 +1,16 @@ +from django.test import TestCase + + +class ImportTestCase(TestCase): + def test_imports(self): + # Make sure we can import the api tests + import tests.management.commands.test_post_downloads_summary + import tests.views.test_api_general + import tests.views.test_compendia + import tests.views.test_dataset + import tests.views.test_dataset_stats + import tests.views.test_processor + import tests.views.test_qn_target + import tests.views.test_search + import tests.test_sentry_middleware + import tests.views.test_stats diff --git a/api/data_refinery_api/test/test_sentry_middleware.py b/api/tests/test_sentry_middleware.py similarity index 96% rename from api/data_refinery_api/test/test_sentry_middleware.py rename to api/tests/test_sentry_middleware.py index edd158e7e..840bea545 100644 --- a/api/data_refinery_api/test/test_sentry_middleware.py +++ b/api/tests/test_sentry_middleware.py @@ -5,8 +5,9 @@ from rest_framework import status from rest_framework.test import APITestCase -from data_refinery_api.test.test_api_general import API_VERSION -from data_refinery_api.views import DatasetView, ExperimentListView +from tests.views.test_api_general import API_VERSION + +from data_refinery_api.views import ExperimentListView class APITestCases(APITestCase): diff --git a/api/tests/views/__init__.py b/api/tests/views/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/api/data_refinery_api/test/test_api_general.py b/api/tests/views/test_api_general.py similarity index 93% rename from api/data_refinery_api/test/test_api_general.py rename to api/tests/views/test_api_general.py index 7b48cc803..8d0b76358 100644 --- a/api/data_refinery_api/test/test_api_general.py +++ b/api/tests/views/test_api_general.py @@ -205,7 +205,7 @@ def setUp(self): return def tearDown(self): - """ Good bye """ + """Good bye""" cache.clear() Experiment.objects.all().delete() ExperimentAnnotation.objects.all().delete() @@ -256,11 +256,12 @@ def test_all_endpoints(self): response = self.client.get(reverse("survey_jobs", kwargs={"version": API_VERSION})) self.assertEqual(response.status_code, status.HTTP_200_OK) - self.assertFalse(response.data["results"][0]["is_queued"]) + job = response.data["results"][0] + self.assertFalse(job["is_queued"]) cache.clear() response = self.client.get( - reverse("survey_jobs", kwargs={"version": API_VERSION}) + "1/" # change back + reverse("survey_jobs_detail", kwargs={"id": job["id"], "version": API_VERSION}) ) self.assertEqual(response.status_code, status.HTTP_200_OK) self.assertFalse(response.data["is_queued"]) @@ -268,16 +269,15 @@ def test_all_endpoints(self): response = self.client.get(reverse("downloader_jobs", kwargs={"version": API_VERSION})) self.assertEqual(response.status_code, status.HTTP_200_OK) - self.assertFalse(response.data["results"][0]["is_queued"]) + job = response.data["results"][0] + self.assertFalse(job["is_queued"]) cache.clear() - # Don't know the best way to deal with this, but since the - # other tests in different files create objects which are then - # deleted, the new objects from these tests will have - # different IDs. In this case, since this file is ran first, - # the IDs are 1, but this may be a problem in the future. response = self.client.get( - reverse("downloader_jobs", kwargs={"version": API_VERSION}) + "1/" # change back + reverse( + "downloader_jobs_detail", + kwargs={"id": job["id"], "version": API_VERSION}, + ) ) self.assertEqual(response.status_code, status.HTTP_200_OK) self.assertFalse(response.data["is_queued"]) @@ -285,11 +285,15 @@ def test_all_endpoints(self): response = self.client.get(reverse("processor_jobs", kwargs={"version": API_VERSION})) self.assertEqual(response.status_code, status.HTTP_200_OK) - self.assertFalse(response.data["results"][0]["is_queued"]) + job = response.data["results"][0] + self.assertFalse(job["is_queued"]) cache.clear() response = self.client.get( - reverse("processor_jobs", kwargs={"version": API_VERSION}) + "1/" + reverse( + "processor_jobs_detail", + kwargs={"id": job["id"], "version": API_VERSION}, + ) ) self.assertEqual(response.status_code, status.HTTP_200_OK) self.assertFalse(response.data["is_queued"]) @@ -301,9 +305,12 @@ def test_all_endpoints(self): response = self.client.get(reverse("results", kwargs={"version": API_VERSION})) self.assertEqual(response.status_code, status.HTTP_200_OK) + result = response.data["results"][0] cache.clear() - response = self.client.get(reverse("results", kwargs={"version": API_VERSION}) + "1/") + response = self.client.get( + reverse("results_detail", kwargs={"id": result["id"], "version": API_VERSION}) + ) self.assertEqual(response.status_code, status.HTTP_200_OK) cache.clear() @@ -311,16 +318,14 @@ def test_all_endpoints(self): self.assertEqual(response.status_code, status.HTTP_200_OK) cache.clear() - response = self.client.get( - reverse("transcriptome_indices", kwargs={"version": API_VERSION}) - + "?organism__name=DANIO_RERIO" + transcriptome_indices_url = reverse( + "transcriptome_indices", kwargs={"version": API_VERSION} ) + response = self.client.get(f"{transcriptome_indices_url}?organism__name=DANIO_RERIO") self.assertEqual(response.status_code, status.HTTP_200_OK) cache.clear() - response = self.client.get( - reverse("transcriptome_indices", kwargs={"version": API_VERSION}) + "?result_id=1" - ) + response = self.client.get(f"{transcriptome_indices_url}?result_id={result['id']}") self.assertEqual(response.status_code, status.HTTP_200_OK) cache.clear() @@ -415,14 +420,16 @@ def test_sample_pagination(self): cache.clear() response = self.client.get( - reverse("samples", kwargs={"version": API_VERSION}), {"limit": 1, "ordering": "-title"} + reverse("samples", kwargs={"version": API_VERSION}), + {"limit": 1, "ordering": "-title"}, ) self.assertEqual(response.status_code, status.HTTP_200_OK) self.assertEqual(response.json()["results"][0]["title"], "789") cache.clear() response = self.client.get( - reverse("samples", kwargs={"version": API_VERSION}), {"limit": 1, "ordering": "title"} + reverse("samples", kwargs={"version": API_VERSION}), + {"limit": 1, "ordering": "title"}, ) self.assertEqual(response.status_code, status.HTTP_200_OK) self.assertEqual(response.json()["results"][0]["title"], "123") @@ -460,7 +467,10 @@ def test_fetching_experiment_samples(self): def test_sample_detail_experiment_accessions(self): response = self.client.get( - reverse("samples_detail", kwargs={"version": API_VERSION, "accession_code": "789"}) + reverse( + "samples_detail", + kwargs={"version": API_VERSION, "accession_code": "789"}, + ) ) self.assertEqual(response.status_code, status.HTTP_200_OK) self.assertEqual(response.json()["experiment_accession_codes"], ["GSE123"]) @@ -483,7 +493,7 @@ def test_fetching_organism_index(self): self.assertEqual(response.status_code, 404) def test_processed_samples_only(self): - """ Don't return unprocessed samples """ + """Don't return unprocessed samples""" experiment = Experiment() experiment.accession_code = "GSX12345" experiment.is_public = True @@ -552,7 +562,8 @@ def test_create_token(self): # Now activate using a second request response = self.client.post( - reverse("token", kwargs={"version": API_VERSION}), content_type="application/json" + reverse("token", kwargs={"version": API_VERSION}), + content_type="application/json", ) self.assertEqual(response.status_code, 201) token = response.json() diff --git a/api/data_refinery_api/test/test_compendia.py b/api/tests/views/test_compendia.py similarity index 97% rename from api/data_refinery_api/test/test_compendia.py rename to api/tests/views/test_compendia.py index 0cfb2c19c..e837677f0 100644 --- a/api/data_refinery_api/test/test_compendia.py +++ b/api/tests/views/test_compendia.py @@ -3,7 +3,8 @@ from django.urls import reverse from rest_framework.test import APITestCase -from data_refinery_api.test.test_api_general import API_VERSION +from tests.views.test_api_general import API_VERSION + from data_refinery_common.models import ( CompendiumResult, ComputationalResult, @@ -76,7 +77,8 @@ def test_compendia(self): drc1.save() response = self.client.get( - reverse("computed_files", kwargs={"version": API_VERSION}), {"is_compendia": True} + reverse("computed_files", kwargs={"version": API_VERSION}), + {"is_compendia": True}, ) response_json = response.json()["results"] self.assertEqual(3, len(response_json)) @@ -124,7 +126,8 @@ def test_organism_has_compendia(self): self.assertEqual(2, len(response_json)) response = self.client.get( - reverse("organisms", kwargs={"version": API_VERSION}), {"has_compendia": True}, + reverse("organisms", kwargs={"version": API_VERSION}), + {"has_compendia": True}, ) response_json = response.json()["results"] self.assertEqual(1, len(response_json)) diff --git a/api/data_refinery_api/test/test_dataset.py b/api/tests/views/test_dataset.py similarity index 97% rename from api/data_refinery_api/test/test_dataset.py rename to api/tests/views/test_dataset.py index e360c8407..bf6e7a4ea 100644 --- a/api/data_refinery_api/test/test_dataset.py +++ b/api/tests/views/test_dataset.py @@ -6,7 +6,8 @@ from rest_framework import status from rest_framework.test import APITestCase -from data_refinery_api.test.test_api_general import API_VERSION +from tests.views.test_api_general import API_VERSION + from data_refinery_common.models import ( ComputationalResult, ComputationalResultAnnotation, @@ -205,7 +206,12 @@ def test_create_dataset_fails_without_email(self): # Good, except for empty email. jdata = json.dumps( - {"start": True, "data": {"GSE123": ["789"]}, "token_id": token_id, "email_address": "",} + { + "start": True, + "data": {"GSE123": ["789"]}, + "token_id": token_id, + "email_address": "", + } ) response = self.client.post( reverse("create_dataset", kwargs={"version": API_VERSION}), @@ -328,7 +334,8 @@ def test_dataset_adding_non_downloadable_samples_fails(self): ) self.assertEqual(response.status_code, 400) self.assertIn( - "Non-downloadable sample(s) in dataset", response.json()["message"], + "Non-downloadable sample(s) in dataset", + response.json()["message"], ) self.assertEqual(response.json()["details"], ["456"]) cache.clear() @@ -341,7 +348,8 @@ def test_dataset_adding_non_downloadable_samples_fails(self): content_type="application/json", ) self.assertIn( - "Sample(s) in dataset do not exist on refine", response.json()["message"], + "Sample(s) in dataset do not exist on refine", + response.json()["message"], ) self.assertEqual(response.status_code, 400) cache.clear() @@ -374,7 +382,8 @@ def test_dataset_adding_non_downloadable_samples_fails(self): ) self.assertEqual(response.status_code, 400) self.assertIn( - "Sample(s) in dataset are missing quant.sf files", response.json()["message"], + "Sample(s) in dataset are missing quant.sf files", + response.json()["message"], ) self.assertEqual(response.json()["details"], ["456"]) cache.clear() @@ -394,7 +403,8 @@ def test_dataset_adding_non_downloadable_samples_fails(self): ) self.assertEqual(response.status_code, 400) self.assertIn( - "Experiment(s) in dataset have zero downloadable samples", response.json()["message"], + "Experiment(s) in dataset have zero downloadable samples", + response.json()["message"], ) self.assertEqual(response.json()["details"], ["GSE123"]) cache.clear() diff --git a/api/data_refinery_api/test/test_dataset_stats.py b/api/tests/views/test_dataset_stats.py similarity index 96% rename from api/data_refinery_api/test/test_dataset_stats.py rename to api/tests/views/test_dataset_stats.py index 6c14181d8..7a0633e69 100644 --- a/api/data_refinery_api/test/test_dataset_stats.py +++ b/api/tests/views/test_dataset_stats.py @@ -1,10 +1,10 @@ import json from django.urls import reverse -from rest_framework import status from rest_framework.test import APITestCase -from data_refinery_api.test.test_api_general import API_VERSION +from tests.views.test_api_general import API_VERSION + from data_refinery_common.models import ( Experiment, ExperimentOrganismAssociation, @@ -20,7 +20,7 @@ def setUp(self): self.homo_sapiens.save() def test_dataset_stats(self): - """ Test the dataset stats endpoint """ + """Test the dataset stats endpoint""" gallus_gallus = Organism(name="GALLUS_GALLUS", taxonomy_id=9031, is_scientific_name=True) gallus_gallus.save() diff --git a/api/data_refinery_api/test/test_processor.py b/api/tests/views/test_processor.py similarity index 98% rename from api/data_refinery_api/test/test_processor.py rename to api/tests/views/test_processor.py index f5f49d8db..88932e313 100644 --- a/api/data_refinery_api/test/test_processor.py +++ b/api/tests/views/test_processor.py @@ -2,7 +2,8 @@ from rest_framework import status from rest_framework.test import APITestCase -from data_refinery_api.test.test_api_general import API_VERSION +from tests.views.test_api_general import API_VERSION + from data_refinery_common.models import ( ComputationalResult, Organism, diff --git a/api/data_refinery_api/test/test_qn_target.py b/api/tests/views/test_qn_target.py similarity index 96% rename from api/data_refinery_api/test/test_qn_target.py rename to api/tests/views/test_qn_target.py index 7443c731b..b03af1e3b 100644 --- a/api/data_refinery_api/test/test_qn_target.py +++ b/api/tests/views/test_qn_target.py @@ -1,7 +1,8 @@ from django.urls import reverse from rest_framework.test import APITestCase -from data_refinery_api.test.test_api_general import API_VERSION +from tests.views.test_api_general import API_VERSION + from data_refinery_common.models import ComputationalResult, ComputationalResultAnnotation, Organism diff --git a/api/data_refinery_api/test/test_search.py b/api/tests/views/test_search.py similarity index 97% rename from api/data_refinery_api/test/test_search.py rename to api/tests/views/test_search.py index a7a5c05b6..f28c8ae9b 100644 --- a/api/data_refinery_api/test/test_search.py +++ b/api/tests/views/test_search.py @@ -6,7 +6,8 @@ from django.urls import reverse from rest_framework.test import APITestCase -from data_refinery_api.test.test_api_general import API_VERSION +from tests.views.test_api_general import API_VERSION + from data_refinery_common.models import ( ComputationalResult, ComputationalResultAnnotation, @@ -66,7 +67,11 @@ def setUpClass(cls): sample.accession_code = "123" sample.save() - organism = Organism(name=ECOLI_STRAIN_NAME, taxonomy_id=879462, is_scientific_name=True,) + organism = Organism( + name=ECOLI_STRAIN_NAME, + taxonomy_id=879462, + is_scientific_name=True, + ) organism.save() sample = Sample() diff --git a/api/data_refinery_api/test/test_stats.py b/api/tests/views/test_stats.py similarity index 98% rename from api/data_refinery_api/test/test_stats.py rename to api/tests/views/test_stats.py index a724ef5c9..28426182f 100644 --- a/api/data_refinery_api/test/test_stats.py +++ b/api/tests/views/test_stats.py @@ -4,7 +4,8 @@ from django.urls import reverse from rest_framework.test import APITestCase -from data_refinery_api.test.test_api_general import API_VERSION +from tests.views.test_api_general import API_VERSION + from data_refinery_api.views.stats import get_batch_jobs_breakdown QUEUE_NAMES = [ diff --git a/scripts/common.sh b/scripts/common.sh index db91ab9a1..612de1a7b 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -20,7 +20,7 @@ get_docker_es_ip_address () { # then exit with the appropriate code. # This is done a function so arguments to the tests can be passed through. run_tests_with_coverage () { - COVERAGE="coverage run --source=\".\" manage.py test --no-input $*; exit_code=\$?;" + COVERAGE="coverage run --source=\".\" manage.py test --settings=tests.settings --no-input $*; exit_code=\$?;" SAVE_REPORT="coverage xml -o data_store/coverage.xml;" PRINT_REPORT="coverage report -m;" RETURN="exit \$exit_code" diff --git a/setup.cfg b/setup.cfg index 677a0f31c..8b9a8a747 100644 --- a/setup.cfg +++ b/setup.cfg @@ -1,20 +1,26 @@ +[coverage:report] +include = + data_refinery_api/* +omit = + data_refinery_api/wsgi.py + [pep8] -max-line-length: 100 +max-line-length = 100 [flake8] -max-line-length: 100 ignore = E203 +max-line-length = 100 [isort] # Settings for compatibility with black. -line_length = 100 -use_parentheses = True -multi_line_output = 3 -include_trailing_comma = True -combine_as_imports = True -known_django=django, rest_framework -known_first_party=data_refinery_api,data_refinery_common,data_refinery_foreman,data_refinery_workers -default_section=THIRDPARTY -sections=FUTURE,STDLIB,DJANGO,THIRDPARTY,FIRSTPARTY,LOCALFOLDER +combine_as_imports = true combine_star = true +default_section = THIRDPARTY +include_trailing_comma = true +known_django = django,rest_framework +known_first_party = data_refinery_api,data_refinery_common,data_refinery_foreman,data_refinery_workers length_sort = false +line_length = 100 +multi_line_output = 3 +sections = FUTURE,STDLIB,DJANGO,THIRDPARTY,FIRSTPARTY,LOCALFOLDER +use_parentheses = true From a3a21bf261e8744d10e1b099bde66e1854a7b784 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 24 Oct 2022 17:48:37 -0700 Subject: [PATCH 032/220] Update `validate_dataset` to allow for experiments with duplicate samples. Change some checks to more readable versions. Use sets instead of lists for entity IDs tracking (accessions, experiments). Get rid of str + str. --- api/data_refinery_api/views/dataset.py | 34 +++++++------- api/tests/views/test_dataset.py | 62 +++++++++++++++++++++++++- 2 files changed, 78 insertions(+), 18 deletions(-) diff --git a/api/data_refinery_api/views/dataset.py b/api/data_refinery_api/views/dataset.py index c3699101c..87d4c69ed 100644 --- a/api/data_refinery_api/views/dataset.py +++ b/api/data_refinery_api/views/dataset.py @@ -75,60 +75,60 @@ def validate_dataset(data): if data.get("data") is None or type(data["data"]) != dict: raise InvalidData("`data` must be a dict of lists.") - if data.get("start") and len(data["data"]) == 0: + if data.get("start") and not data["data"]: raise InvalidData("`data` must contain at least one experiment.") - accessions = [] - non_downloadable_experiments = [] + accessions = set() + non_downloadable_experiments = set() + for key, value in data["data"].items(): if type(value) != list: - raise InvalidData("`data` must be a dict of lists. Problem with `" + str(key) + "`") + raise InvalidData(f"`data` must be a dict of lists. Problem with `{key}`") - if len(value) < 1: + if not value: raise InvalidData( - "`data` must be a dict of lists, each with one or more elements. Problem with `" - + str(key) - + "`" + "`data` must be a dict of lists, each with one or more elements. " + f"Problem with `{key}`" ) if len(value) != len(set(value)): - raise InvalidData("Duplicate values detected in " + str(value)) + raise InvalidData(f"Duplicate values detected in {value}") # If they want "ALL", just make sure that the experiment has at least one downloadable sample if value == ["ALL"]: if not experiment_has_downloadable_samples( key, quant_sf_only=data.get("quant_sf_only", False) ): - non_downloadable_experiments.append(key) + non_downloadable_experiments.add(key) # Otherwise, we will check that all the samples they requested are downloadable else: - accessions.extend(value) + accessions.update(value) - if len(non_downloadable_experiments) != 0: + if non_downloadable_experiments: raise InvalidData( message="Experiment(s) in dataset have zero downloadable samples. See `details` for a full list", details=non_downloadable_experiments, ) - if len(accessions) == 0: + if not accessions: return samples = Sample.public_objects.filter(accession_code__in=accessions) if samples.count() != len(accessions): raise InvalidData( message="Sample(s) in dataset do not exist on refine.bio. See `details` for a full list", - details=list(set(accessions) - set(s.accession_code for s in samples)), + details=list(accessions - set(s.accession_code for s in samples)), ) - if data.get("quant_sf_only", False): + if data.get("quant_sf_only"): samples_without_quant_sf = samples.exclude( # Exclude samples that have at least one uploaded quant.sf file associated with them results__computedfile__filename="quant.sf", results__computedfile__s3_key__isnull=False, results__computedfile__s3_bucket__isnull=False, ) - if samples_without_quant_sf.count() > 0: + if samples_without_quant_sf.count(): raise InvalidData( message="Sample(s) in dataset are missing quant.sf files. See `details` for a full list", details=[s.accession_code for s in samples_without_quant_sf], @@ -136,7 +136,7 @@ def validate_dataset(data): else: unprocessed_samples = samples.exclude(is_processed=True) - if unprocessed_samples.count() > 0: + if unprocessed_samples.count(): raise InvalidData( message="Non-downloadable sample(s) in dataset. See `details` for a full list", details=[s.accession_code for s in unprocessed_samples], diff --git a/api/tests/views/test_dataset.py b/api/tests/views/test_dataset.py index bf6e7a4ea..f2a65bb55 100644 --- a/api/tests/views/test_dataset.py +++ b/api/tests/views/test_dataset.py @@ -348,7 +348,7 @@ def test_dataset_adding_non_downloadable_samples_fails(self): content_type="application/json", ) self.assertIn( - "Sample(s) in dataset do not exist on refine", + "Sample(s) in dataset do not exist on refine.bio", response.json()["message"], ) self.assertEqual(response.status_code, 400) @@ -707,3 +707,63 @@ def test_create_dataset_notify_me(self): ds.refresh_from_db() self.assertFalse(ds.notify_me) + + def test_add_dataset_multiple_experiment_sample(self): + response = self.client.post( + reverse("token", kwargs={"version": API_VERSION}), + json.dumps({"is_activated": True}), + content_type="application/json", + ) + token_id = response.json()["id"] + + response = self.client.post( + reverse("create_dataset", kwargs={"version": API_VERSION}), + json.dumps( + { + "data": { + "GSE000": ["789"], + }, + "token_id": token_id, + } + ), + content_type="application/json", + ) + self.assertEqual(response.status_code, 201) + + data_set_id = response.json()["id"] + data = { + "GSE000": ["789"], + "GSE123": ["789"], + } + response = self.client.put( + reverse("dataset", kwargs={"id": data_set_id, "version": API_VERSION}), + json.dumps( + { + "data": data, + "token_id": token_id, + } + ), + content_type="application/json", + ) + self.assertEqual(response.status_code, 200) + + data_set = Dataset.objects.get(id=data_set_id) + self.assertEqual(data_set.data, data) + + data = { + "GSE000": ["789"], + } + response = self.client.put( + reverse("dataset", kwargs={"id": data_set_id, "version": API_VERSION}), + json.dumps( + { + "data": data, + "token_id": token_id, + } + ), + content_type="application/json", + ) + self.assertEqual(response.status_code, 200) + + data_set.refresh_from_db() + self.assertEqual(data_set.data, data) From d2d997c37ea3888775b1f69ef0b3d986e63f1de5 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 31 Oct 2022 16:48:41 -0700 Subject: [PATCH 033/220] Optimize rna-seq accessions gathering. --- .../gatherer/agents/rnaseq_agent.py | 33 +++++++------------ 1 file changed, 12 insertions(+), 21 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py index 9f2f54e3a..574a91109 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py +++ b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py @@ -34,10 +34,7 @@ class RNASeqAgent(AccessionAgentBase): def __str__(self): return "RNA-Seq accession agent" - def __str__(self): - return "RNA-Seq accession agent" - - def build_query(self, taxon_id: str = None) -> str: + def build_query(self, taxon_ids: tuple = None) -> str: """ Returns a query to use for getting specific taxon ID accessions. Some special characters must remain unquoted. @@ -69,6 +66,7 @@ def build_query(self, taxon_id: str = None) -> str: ) instrument_models = OR.join((f'instrument_model="{im}"' for im in instrument_models)) + taxon_ids = OR.join((f"tax_eq({taxon_id})" for taxon_id in taxon_ids)) conditions = [ # Relevant date fields: collection_date, collection_date_submitted, # first_public, last_updated. @@ -79,8 +77,8 @@ def build_query(self, taxon_id: str = None) -> str: 'library_strategy="RNA-Seq"', ] - if taxon_id: - conditions.append(f"tax_eq({taxon_id})") + if taxon_ids: + conditions.append(f"({taxon_ids})") elif self.keyword: search_fields = ( "assembly_software", @@ -118,26 +116,19 @@ def collect_data(self) -> Set[str]: accessions = set() if self.ids: - logger.debug( - f"Getting RNA-Seq entries by taxon ID(s): " + logger.info( + f"Getting {self.count or 'all'} RNA-Seq entries by taxon ID(s): " f"{', '.join((str(i) for i in self.ids))} for [{self.since} - {self.until}] range." ) - total = len(self.ids) - for idx, taxon_id in enumerate(self.ids): - if self.count and len(accessions) >= self.count: - break - - if total > 1: - logger.debug(f"Getting entries for taxon ID {taxon_id}, {idx + 1} of {total}.") - accessions.update(self.fetch_data(taxon_id=taxon_id)) + accessions.update(self.fetch_data(taxon_ids=self.ids)) elif self.keyword: - logger.debug( + logger.info( f'Getting RNA-Seq entries by "{self.keyword}" keyword ' f"for [{self.since} - {self.until}] range." ) accessions.update(self.fetch_data()) elif self.organism: - logger.debug( + logger.info( f'Getting entries by "{self.organism}" organism ' f"for [{self.since} - {self.until}] range." ) @@ -145,7 +136,7 @@ def collect_data(self) -> Set[str]: return accessions - def fetch_data(self, taxon_id=None) -> Set[str]: + def fetch_data(self, taxon_ids=None) -> Set[str]: """ Retrieves accessions from API search endpoint. The API allows to set limit to 0 (get all in one request) but we do @@ -171,14 +162,14 @@ def get_response(url, **kwargs): "format": "json", "limit": self.DATA_CHUNK_SIZE, "offset": 0, - "query": self.build_query(taxon_id=taxon_id), + "query": self.build_query(taxon_ids=taxon_ids), "result": "read_study", "sortFields": fields, } is_done = False while not is_done: - logger.debug( + logger.info( f"Processing entries {data['offset'] + 1} - {data['offset'] + self.DATA_CHUNK_SIZE}" ) entries = () From 8b5808a8c782060f05565f31c8009f43295ec059 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 31 Oct 2022 16:52:09 -0700 Subject: [PATCH 034/220] Revert "Optimize rna-seq accessions gathering." This reverts commit d2d997c37ea3888775b1f69ef0b3d986e63f1de5. --- .../gatherer/agents/rnaseq_agent.py | 33 ++++++++++++------- 1 file changed, 21 insertions(+), 12 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py index 574a91109..9f2f54e3a 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py +++ b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py @@ -34,7 +34,10 @@ class RNASeqAgent(AccessionAgentBase): def __str__(self): return "RNA-Seq accession agent" - def build_query(self, taxon_ids: tuple = None) -> str: + def __str__(self): + return "RNA-Seq accession agent" + + def build_query(self, taxon_id: str = None) -> str: """ Returns a query to use for getting specific taxon ID accessions. Some special characters must remain unquoted. @@ -66,7 +69,6 @@ def build_query(self, taxon_ids: tuple = None) -> str: ) instrument_models = OR.join((f'instrument_model="{im}"' for im in instrument_models)) - taxon_ids = OR.join((f"tax_eq({taxon_id})" for taxon_id in taxon_ids)) conditions = [ # Relevant date fields: collection_date, collection_date_submitted, # first_public, last_updated. @@ -77,8 +79,8 @@ def build_query(self, taxon_ids: tuple = None) -> str: 'library_strategy="RNA-Seq"', ] - if taxon_ids: - conditions.append(f"({taxon_ids})") + if taxon_id: + conditions.append(f"tax_eq({taxon_id})") elif self.keyword: search_fields = ( "assembly_software", @@ -116,19 +118,26 @@ def collect_data(self) -> Set[str]: accessions = set() if self.ids: - logger.info( - f"Getting {self.count or 'all'} RNA-Seq entries by taxon ID(s): " + logger.debug( + f"Getting RNA-Seq entries by taxon ID(s): " f"{', '.join((str(i) for i in self.ids))} for [{self.since} - {self.until}] range." ) - accessions.update(self.fetch_data(taxon_ids=self.ids)) + total = len(self.ids) + for idx, taxon_id in enumerate(self.ids): + if self.count and len(accessions) >= self.count: + break + + if total > 1: + logger.debug(f"Getting entries for taxon ID {taxon_id}, {idx + 1} of {total}.") + accessions.update(self.fetch_data(taxon_id=taxon_id)) elif self.keyword: - logger.info( + logger.debug( f'Getting RNA-Seq entries by "{self.keyword}" keyword ' f"for [{self.since} - {self.until}] range." ) accessions.update(self.fetch_data()) elif self.organism: - logger.info( + logger.debug( f'Getting entries by "{self.organism}" organism ' f"for [{self.since} - {self.until}] range." ) @@ -136,7 +145,7 @@ def collect_data(self) -> Set[str]: return accessions - def fetch_data(self, taxon_ids=None) -> Set[str]: + def fetch_data(self, taxon_id=None) -> Set[str]: """ Retrieves accessions from API search endpoint. The API allows to set limit to 0 (get all in one request) but we do @@ -162,14 +171,14 @@ def get_response(url, **kwargs): "format": "json", "limit": self.DATA_CHUNK_SIZE, "offset": 0, - "query": self.build_query(taxon_ids=taxon_ids), + "query": self.build_query(taxon_id=taxon_id), "result": "read_study", "sortFields": fields, } is_done = False while not is_done: - logger.info( + logger.debug( f"Processing entries {data['offset'] + 1} - {data['offset'] + self.DATA_CHUNK_SIZE}" ) entries = () From 6ee639400058ba94d7db6e0754f065d8b48c4164 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 31 Oct 2022 16:59:06 -0700 Subject: [PATCH 035/220] Update logging. --- .../management/commands/gather_accessions.py | 23 +++++++++---------- 1 file changed, 11 insertions(+), 12 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index f3f644a91..3e50dac28 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -14,7 +14,6 @@ from django.template.defaultfilters import pluralize from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models import gathered_accession from data_refinery_common.models.gathered_accession import GatheredAccession from data_refinery_foreman.gatherer.agents.ae_agent import AEAgent from data_refinery_foreman.gatherer.agents.geo_agent import GEOAgent @@ -203,24 +202,22 @@ def handle(self, *args, **options): agents.append(cls(options)) gathered_accessions = set() - gathered_accessions_count = 0 for agent in agents: agent_accessions = agent.collect_data() agent_accessions_count = len(agent_accessions) gathered_accessions.update(agent_accessions) - gathered_accessions_count += agent_accessions_count logger.info( - f"{agent} gathered {agent_accessions_count} " - f"new accession{pluralize(gathered_accessions_count)} since {options['since']}." + f"Since {options['since']} {agent} has gathered {agent_accessions_count} " + f"new accession{pluralize(agent_accessions_count)}." ) gathered_accessions = sorted( # Sort the resulting list. - (ga for ga in gathered_accessions if self.RE_ACCESSION.match(ga.code)), + (ga for ga in gathered_accessions if self.RE_ACCESSION.match(ga.accession_code)), key=lambda ga: ( - self.RE_ACCESSION.match(ga.code).group(1), - int(self.RE_ACCESSION.match(ga.code).group(2)), + self.RE_ACCESSION.match(ga.accession_code).group(1), + int(self.RE_ACCESSION.match(ga.accession_code).group(2)), ), ) # Limit the number of output entries. @@ -229,10 +226,12 @@ def handle(self, *args, **options): ) agents_count = len(agents) + gathered_accessions_count = len(gathered_accessions) logger.info( - f"{gathered_accessions_count} new accession{pluralize(gathered_accessions_count)} " - f"w{pluralize(gathered_accessions_count, 'as,ere')} gathered by {agents_count} " - f"accession agent{pluralize(agents_count)} since {options['since']}." + f"Since {options['since']} {gathered_accessions_count} " + f"new accession{pluralize(gathered_accessions_count)} " + f"h{pluralize(gathered_accessions_count, 'as,ave')} been gathered by {agents_count} " + f"accession agent{pluralize(agents_count)}." ) if options["dry_run"]: @@ -247,7 +246,7 @@ def handle(self, *args, **options): added_accessions_count = len(gathered_accessions) logger.info( f"{added_accessions_count} new accession{pluralize(added_accessions_count)} " - f"w{pluralize(added_accessions_count, 'as,ere')} added to the database. " + f"h{pluralize(added_accessions_count, 'as,ave')} been added to the database. " ) except IntegrityError as e: logger.exception(f"Could not save new accessions to the database: {e}") From 03e43e706e658ce502c24b7e935d460a8638d7da Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 1 Nov 2022 18:39:37 -0700 Subject: [PATCH 036/220] Move all tests to tests directory. Add separate settings file (tests/settings.py). Refactor import according to the new structure. --- .pre-commit-config.yaml | 2 +- workers/tests/__init__.py | 0 workers/tests/downloaders/__init__.py | 0 .../downloaders/test_array_express.py | 0 .../downloaders/test_geo.py | 7 ++--- .../downloaders/test_imports.py | 0 .../downloaders/test_sra.py | 0 .../downloaders/test_transcriptome_index.py | 0 workers/tests/processors/__init__.py | 0 .../processors/test_agilent.py | 0 .../processors/test_array_express.py | 2 +- .../processors/test_compendia.py | 2 +- .../processors/test_create_quantpendia.py | 0 .../processors/test_illumina.py | 29 +++++++++++++++---- .../processors/test_imports.py | 22 +++++++------- .../processors/test_janitor.py | 2 +- .../processors/test_no_op.py | 0 .../processors/test_qn_reference.py | 0 .../processors/test_salmon.py | 7 ++--- .../processors/test_smasher.py | 5 +--- .../processors/test_transcriptome_index.py | 5 +++- .../processors/test_tximport.R | 0 .../processors/test_utils.py | 0 workers/tests/settings.py | 1 + .../testing_utils.py => tests/utils.py} | 0 25 files changed, 49 insertions(+), 35 deletions(-) create mode 100644 workers/tests/__init__.py create mode 100644 workers/tests/downloaders/__init__.py rename workers/{data_refinery_workers => tests}/downloaders/test_array_express.py (100%) rename workers/{data_refinery_workers => tests}/downloaders/test_geo.py (98%) rename workers/{data_refinery_workers => tests}/downloaders/test_imports.py (100%) rename workers/{data_refinery_workers => tests}/downloaders/test_sra.py (100%) rename workers/{data_refinery_workers => tests}/downloaders/test_transcriptome_index.py (100%) create mode 100644 workers/tests/processors/__init__.py rename workers/{data_refinery_workers => tests}/processors/test_agilent.py (100%) rename workers/{data_refinery_workers => tests}/processors/test_array_express.py (98%) rename workers/{data_refinery_workers => tests}/processors/test_compendia.py (99%) rename workers/{data_refinery_workers => tests}/processors/test_create_quantpendia.py (100%) rename workers/{data_refinery_workers => tests}/processors/test_illumina.py (96%) rename workers/{data_refinery_workers => tests}/processors/test_imports.py (62%) rename workers/{data_refinery_workers => tests}/processors/test_janitor.py (99%) rename workers/{data_refinery_workers => tests}/processors/test_no_op.py (100%) rename workers/{data_refinery_workers => tests}/processors/test_qn_reference.py (100%) rename workers/{data_refinery_workers => tests}/processors/test_salmon.py (99%) rename workers/{data_refinery_workers => tests}/processors/test_smasher.py (99%) rename workers/{data_refinery_workers => tests}/processors/test_transcriptome_index.py (98%) rename workers/{data_refinery_workers => tests}/processors/test_tximport.R (100%) rename workers/{data_refinery_workers => tests}/processors/test_utils.py (100%) create mode 100644 workers/tests/settings.py rename workers/{data_refinery_workers/processors/testing_utils.py => tests/utils.py} (100%) diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index d024704da..b651ce24a 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -22,7 +22,7 @@ repos: - id: isort - repo: https://github.com/psf/black - rev: 19.10b0 + rev: 22.3.0 hooks: - id: black args: [--line-length=100] diff --git a/workers/tests/__init__.py b/workers/tests/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/workers/tests/downloaders/__init__.py b/workers/tests/downloaders/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/workers/data_refinery_workers/downloaders/test_array_express.py b/workers/tests/downloaders/test_array_express.py similarity index 100% rename from workers/data_refinery_workers/downloaders/test_array_express.py rename to workers/tests/downloaders/test_array_express.py diff --git a/workers/data_refinery_workers/downloaders/test_geo.py b/workers/tests/downloaders/test_geo.py similarity index 98% rename from workers/data_refinery_workers/downloaders/test_geo.py rename to workers/tests/downloaders/test_geo.py index 9d951a33b..8f6ebc2de 100644 --- a/workers/data_refinery_workers/downloaders/test_geo.py +++ b/workers/tests/downloaders/test_geo.py @@ -90,7 +90,7 @@ def test_download_and_extract_file(self): @tag("downloaders") def test_download_aspera_and_ftp(self): - """ Tests the main 'download_geo' function. """ + """Tests the main 'download_geo' function.""" dlj = DownloaderJob() dlj.accession_code = "GSE22427" @@ -158,7 +158,7 @@ def test_download_aspera_and_ftp(self): @tag("downloaders") def test_download_geo(self): - """ Tests the main 'download_geo' function. """ + """Tests the main 'download_geo' function.""" dlj = DownloaderJob() dlj.accession_code = "GSE22427" @@ -217,8 +217,7 @@ def test_download_geo(self): @tag("downloaders") def test_no_rnaseq(self): - """Makes sure that no RNA-Seq data gets downloaded even if there's a job for it. - """ + """Makes sure that no RNA-Seq data gets downloaded even if there's a job for it.""" dlj = DownloaderJob() dlj.accession_code = "GSE103217" dlj.save() diff --git a/workers/data_refinery_workers/downloaders/test_imports.py b/workers/tests/downloaders/test_imports.py similarity index 100% rename from workers/data_refinery_workers/downloaders/test_imports.py rename to workers/tests/downloaders/test_imports.py diff --git a/workers/data_refinery_workers/downloaders/test_sra.py b/workers/tests/downloaders/test_sra.py similarity index 100% rename from workers/data_refinery_workers/downloaders/test_sra.py rename to workers/tests/downloaders/test_sra.py diff --git a/workers/data_refinery_workers/downloaders/test_transcriptome_index.py b/workers/tests/downloaders/test_transcriptome_index.py similarity index 100% rename from workers/data_refinery_workers/downloaders/test_transcriptome_index.py rename to workers/tests/downloaders/test_transcriptome_index.py diff --git a/workers/tests/processors/__init__.py b/workers/tests/processors/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/workers/data_refinery_workers/processors/test_agilent.py b/workers/tests/processors/test_agilent.py similarity index 100% rename from workers/data_refinery_workers/processors/test_agilent.py rename to workers/tests/processors/test_agilent.py diff --git a/workers/data_refinery_workers/processors/test_array_express.py b/workers/tests/processors/test_array_express.py similarity index 98% rename from workers/data_refinery_workers/processors/test_array_express.py rename to workers/tests/processors/test_array_express.py index 70247b4a2..ee447e0dd 100644 --- a/workers/data_refinery_workers/processors/test_array_express.py +++ b/workers/tests/processors/test_array_express.py @@ -5,6 +5,7 @@ import pandas as pd import scipy.stats +from tests.utils import assertMostlyAgrees from data_refinery_common.models import ( ComputationalResult, @@ -14,7 +15,6 @@ ProcessorJobOriginalFileAssociation, ) from data_refinery_workers.processors import array_express -from data_refinery_workers.processors.testing_utils import assertMostlyAgrees def prepare_ba_job(): diff --git a/workers/data_refinery_workers/processors/test_compendia.py b/workers/tests/processors/test_compendia.py similarity index 99% rename from workers/data_refinery_workers/processors/test_compendia.py rename to workers/tests/processors/test_compendia.py index 1c4234181..85373a6a7 100644 --- a/workers/data_refinery_workers/processors/test_compendia.py +++ b/workers/tests/processors/test_compendia.py @@ -11,6 +11,7 @@ import numpy as np import pandas as pd +from tests.utils import ProcessorJobTestCaseMixin from data_refinery_common.enums import PipelineEnum, ProcessorPipeline from data_refinery_common.models import ( @@ -29,7 +30,6 @@ SampleResultAssociation, ) from data_refinery_workers.processors import create_compendia, utils -from data_refinery_workers.processors.testing_utils import ProcessorJobTestCaseMixin def create_sample_for_experiment(sample_info: Dict, experiment: Experiment) -> Sample: diff --git a/workers/data_refinery_workers/processors/test_create_quantpendia.py b/workers/tests/processors/test_create_quantpendia.py similarity index 100% rename from workers/data_refinery_workers/processors/test_create_quantpendia.py rename to workers/tests/processors/test_create_quantpendia.py diff --git a/workers/data_refinery_workers/processors/test_illumina.py b/workers/tests/processors/test_illumina.py similarity index 96% rename from workers/data_refinery_workers/processors/test_illumina.py rename to workers/tests/processors/test_illumina.py index de2ff9d0e..44e568daa 100644 --- a/workers/data_refinery_workers/processors/test_illumina.py +++ b/workers/tests/processors/test_illumina.py @@ -7,6 +7,8 @@ from django.test import TestCase, tag +from tests import utils + from data_refinery_common.enums import PipelineEnum from data_refinery_common.models import ( Organism, @@ -18,7 +20,7 @@ Sample, SampleAnnotation, ) -from data_refinery_workers.processors import illumina, testing_utils, utils +from data_refinery_workers.processors import illumina, utils def prepare_illumina_job(job_info: Dict) -> ProcessorJob: @@ -318,7 +320,10 @@ def test_illumina_quoted_row_names(self): "filename": "GSE33814_trimmed_non-normalized.txt", "absolute_file_path": "/home/user/data_store/raw/TEST/ILLUMINA/GSE33814_trimmed_non-normalized.txt", "organism": organism, - "samples": [("GSM836222", "IMGUS_32"), ("GSM836223", "IMGUS_33"),], + "samples": [ + ("GSM836222", "IMGUS_32"), + ("GSM836223", "IMGUS_33"), + ], } ) @@ -357,12 +362,16 @@ def test_illumina_rows_starting_with_whitespace(self): ( "GSM3071991", "MCF-7 KLHDC7B siRNA knockdown control", - {"description": ["SAMPLE 1"],}, + { + "description": ["SAMPLE 1"], + }, ), ( "GSM3071992", "MCF-7 KLHDC7B siRNA knockdown", - {"description": ["SAMPLE 2"],}, + { + "description": ["SAMPLE 2"], + }, ), ], } @@ -420,7 +429,9 @@ def test_illumina_no_pvalue(self): "filename": "GSE41355_non-normalized.txt", "absolute_file_path": "/home/user/data_store/raw/TEST/ILLUMINA/GSE41355_non-normalized.txt", "organism": organism, - "samples": [("GSM1015436", "IRF3/7 DKO 2"),], + "samples": [ + ("GSM1015436", "IRF3/7 DKO 2"), + ], } ) @@ -445,7 +456,13 @@ def test_illumina_id_ref_column_with_whitespace(self): "absolute_file_path": "/home/user/data_store/raw/TEST/ILLUMINA/GSE100301_non-normalized.txt", "organism": organism, "samples": [ - ("GSM2677583", "22Rv1-tetO-Gal4, replicate 1", {"description": ["SAMPLE 1"],},), + ( + "GSM2677583", + "22Rv1-tetO-Gal4, replicate 1", + { + "description": ["SAMPLE 1"], + }, + ), ], } ) diff --git a/workers/data_refinery_workers/processors/test_imports.py b/workers/tests/processors/test_imports.py similarity index 62% rename from workers/data_refinery_workers/processors/test_imports.py rename to workers/tests/processors/test_imports.py index 5e85071c1..f344a0488 100644 --- a/workers/data_refinery_workers/processors/test_imports.py +++ b/workers/tests/processors/test_imports.py @@ -5,50 +5,50 @@ class ImportTestCase(TestCase): @tag("salmon") def test_salmon_imports(self): # Make sure we can import the salmon tests - import data_refinery_workers.processors.test_salmon + import tests.processors.test_salmon @tag("transcriptome") def test_transcriptome_imports(self): # Make sure we can import the transcriptome tests - import data_refinery_workers.processors.test_transcriptome_index + import tests.processors.test_transcriptome_index @tag("no_op") def test_no_op_imports(self): # Make sure we can import the no_op tests - import data_refinery_workers.processors.test_no_op + import tests.processors.test_no_op @tag("smasher") def test_smasher_imports(self): # Make sure we can import the smasher tests - import data_refinery_workers.processors.test_smasher + import tests.processors.test_smasher @tag("illumina") def test_illumina_imports(self): # Make sure we can import the illumina tests - import data_refinery_workers.processors.test_illumina + import tests.processors.test_illumina @tag("agilent") def test_agilent_imports(self): # Make sure we can import the agilent tests - import data_refinery_workers.processors.test_agilent + import tests.processors.test_agilent @tag("affymetrix") def test_affymetrix_imports(self): # Make sure we can import the affy tests - import data_refinery_workers.processors.test_array_express + import tests.processors.test_array_express @tag("qn") def test_qn_imports(self): # Make sure we can import the qn tests - import data_refinery_workers.processors.test_qn_reference + import tests.processors.test_qn_reference @tag("janitor") def test_janitor_imports(self): # Make sure we can import the janitor tests - import data_refinery_workers.processors.test_janitor + import tests.processors.test_janitor @tag("compendia") def test_compendia_imports(self): # Make sure we can import the compendia tests - import data_refinery_workers.processors.test_compendia - import data_refinery_workers.processors.test_create_quantpendia + import tests.processors.test_compendia + import tests.processors.test_create_quantpendia diff --git a/workers/data_refinery_workers/processors/test_janitor.py b/workers/tests/processors/test_janitor.py similarity index 99% rename from workers/data_refinery_workers/processors/test_janitor.py rename to workers/tests/processors/test_janitor.py index a3ba2aa3a..fb755ca8e 100644 --- a/workers/data_refinery_workers/processors/test_janitor.py +++ b/workers/tests/processors/test_janitor.py @@ -79,7 +79,7 @@ class JanitorTestCase(TestCase): @tag("janitor") @patch("data_refinery_workers.processors.janitor.batch.describe_jobs") def test_janitor(self, mock_describe_jobs): - """ Main tester. """ + """Main tester.""" job = prepare_job() mock_describe_jobs.return_value = {"jobs": [{"jobId": "running_job", "status": "RUNNING"}]} diff --git a/workers/data_refinery_workers/processors/test_no_op.py b/workers/tests/processors/test_no_op.py similarity index 100% rename from workers/data_refinery_workers/processors/test_no_op.py rename to workers/tests/processors/test_no_op.py diff --git a/workers/data_refinery_workers/processors/test_qn_reference.py b/workers/tests/processors/test_qn_reference.py similarity index 100% rename from workers/data_refinery_workers/processors/test_qn_reference.py rename to workers/tests/processors/test_qn_reference.py diff --git a/workers/data_refinery_workers/processors/test_salmon.py b/workers/tests/processors/test_salmon.py similarity index 99% rename from workers/data_refinery_workers/processors/test_salmon.py rename to workers/tests/processors/test_salmon.py index afe00ca03..e2744668b 100644 --- a/workers/data_refinery_workers/processors/test_salmon.py +++ b/workers/tests/processors/test_salmon.py @@ -28,7 +28,6 @@ Sample, SampleComputedFileAssociation, SampleResultAssociation, - SurveyJob, ) from data_refinery_common.utils import get_env_variable from data_refinery_workers.processors import salmon, tximport, utils @@ -496,8 +495,7 @@ def test_salmon_quant_two_samples_single_read(self): @tag("salmon") def test_set_tximport_inputs(self): - """"Tests that tximport only considers RNA-Seq samples from GEO. - """ + """ "Tests that tximport only considers RNA-Seq samples from GEO.""" # Create one experiment and two related samples, based on: # https://www.ncbi.nlm.nih.gov/sra/?term=SRP040623 # (We don't need any original files because @@ -1070,8 +1068,7 @@ def test_early_tximport(self): @tag("salmon") def test_version_filter(self): - """Tests that we don't run tximport on old salmon versions. - """ + """Tests that we don't run tximport on old salmon versions.""" # Accessions SRR5125616-SRR5125620 don't exist in SRA, but we # don't actually want to process them so it's okay. incomplete_accessions = [ diff --git a/workers/data_refinery_workers/processors/test_smasher.py b/workers/tests/processors/test_smasher.py similarity index 99% rename from workers/data_refinery_workers/processors/test_smasher.py rename to workers/tests/processors/test_smasher.py index 379caa144..38e7760df 100644 --- a/workers/data_refinery_workers/processors/test_smasher.py +++ b/workers/tests/processors/test_smasher.py @@ -13,6 +13,7 @@ import pandas as pd import vcr +from tests.utils import ProcessorJobTestCaseMixin from data_refinery_common.models import ( ComputationalResult, @@ -24,19 +25,15 @@ ExperimentSampleAssociation, OntologyTerm, Organism, - OriginalFile, ProcessorJob, ProcessorJobDatasetAssociation, - ProcessorJobOriginalFileAssociation, Sample, SampleAnnotation, SampleAttribute, SampleComputedFileAssociation, SampleResultAssociation, - SurveyJob, ) from data_refinery_workers.processors import smasher, smashing_utils -from data_refinery_workers.processors.testing_utils import ProcessorJobTestCaseMixin def prepare_job(): diff --git a/workers/data_refinery_workers/processors/test_transcriptome_index.py b/workers/tests/processors/test_transcriptome_index.py similarity index 98% rename from workers/data_refinery_workers/processors/test_transcriptome_index.py rename to workers/tests/processors/test_transcriptome_index.py index fb1187b73..c549afa5f 100644 --- a/workers/data_refinery_workers/processors/test_transcriptome_index.py +++ b/workers/tests/processors/test_transcriptome_index.py @@ -121,7 +121,10 @@ def test_process_gtf(self): job_context = transcriptome_index._process_gtf(job_context) transcript_to_gene_ids = pd.read_csv( - job_context["genes_to_transcripts_path"], sep="\t", index_col=1, names=["Gene"], + job_context["genes_to_transcripts_path"], + sep="\t", + index_col=1, + names=["Gene"], )["Gene"] self.assertEqual(transcript_to_gene_ids.get("ENST00000480647", None), "ENSG00000041988") diff --git a/workers/data_refinery_workers/processors/test_tximport.R b/workers/tests/processors/test_tximport.R similarity index 100% rename from workers/data_refinery_workers/processors/test_tximport.R rename to workers/tests/processors/test_tximport.R diff --git a/workers/data_refinery_workers/processors/test_utils.py b/workers/tests/processors/test_utils.py similarity index 100% rename from workers/data_refinery_workers/processors/test_utils.py rename to workers/tests/processors/test_utils.py diff --git a/workers/tests/settings.py b/workers/tests/settings.py new file mode 100644 index 000000000..cb2bd4c16 --- /dev/null +++ b/workers/tests/settings.py @@ -0,0 +1 @@ +from data_refinery_workers.settings import * diff --git a/workers/data_refinery_workers/processors/testing_utils.py b/workers/tests/utils.py similarity index 100% rename from workers/data_refinery_workers/processors/testing_utils.py rename to workers/tests/utils.py From dccc246703eb8d25fbca521ebd625de9fd257298 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 2 Nov 2022 09:11:57 -0700 Subject: [PATCH 037/220] Update workers Docker images: - Optimize and reorder building instructions. - Update Python to v3.8. - Resolve OS level package dependency conflicts. - Add and set up missing OS packages. - Add apt cache clean up instructions. - Use `--ignore-installed` pip flag for better deps management. - --- workers/dockerfiles/Dockerfile.affymetrix | 87 +++++++------- .../dockerfiles/Dockerfile.affymetrix_local | 3 +- workers/dockerfiles/Dockerfile.compendia | 106 +++++++++--------- workers/dockerfiles/Dockerfile.downloaders | 88 ++++++++------- workers/dockerfiles/Dockerfile.illumina | 87 +++++++------- workers/dockerfiles/Dockerfile.no_op | 81 ++++++------- workers/dockerfiles/Dockerfile.salmon | 100 +++++++++-------- workers/dockerfiles/Dockerfile.smasher | 86 +++++++------- workers/dockerfiles/Dockerfile.transcriptome | 61 +++++----- 9 files changed, 362 insertions(+), 337 deletions(-) diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index 33c6e2518..151473ecf 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -1,45 +1,51 @@ FROM ubuntu:20.04 +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG C.UTF-8 + +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + RUN apt-get update -qq RUN apt-get install -y software-properties-common RUN add-apt-repository ppa:apt-fast/stable -RUN apt-get update -qq -RUN apt-get -y install apt-fast -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive +RUN apt-get update -qq +RUN apt-get install -y apt-fast apt-transport-https # The packages related to R are somewhat weird, see the README for more details. - COPY workers/CRAN.gpg . -RUN \ - apt-fast update -qq && \ - apt-get install -y apt-transport-https && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq && \ - apt-fast install -y \ +RUN apt-key add CRAN.gpg +RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list + +RUN apt-fast update -qq && apt-fast install -y \ + build-essential \ + cmake \ + curl \ + cython3 \ ed \ git \ - mercurial \ libcairo-dev \ + libcurl4-openssl-dev \ libedit-dev \ + libpq-dev \ + libssl-dev \ + libxml2-dev \ + llvm-10-dev \ lsb-release \ - python3 \ + mercurial \ + pkg-config \ python3-pip \ + python3 \ python3-dev \ r-base-core \ - r-base-dev \ - libpq-dev \ - libxml2-dev \ - libssl-dev \ - libcurl4-openssl-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* + wget + RUN rm CRAN.gpg +RUN apt-get clean; rm -rf /var/lib/apt/lists/* +RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user @@ -47,41 +53,29 @@ WORKDIR /home/user ENV R_LIBS "/usr/local/lib/R/site-library" COPY common/install_devtools.R . - RUN Rscript install_devtools.R COPY workers/R_dependencies/affymetrix/dependencies.R . RUN Rscript dependencies.R COPY workers/affymetrix_dependencies.R . -COPY workers/install_ensg_pkgs.R . - RUN Rscript affymetrix_dependencies.R -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - RUN pip3 install pip --upgrade +RUN pip3 install setuptools --upgrade -RUN pip3 install setuptools --upgrade && \ - rm -rf /root/.cache - -COPY config/ config/ -COPY .boto .boto - -COPY common/dist/data-refinery-common-* common/ +# Install this one here instead of via requirements.txt because not +# all processors need it. +RUN pip3 install rpy2==3.4.5 +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install -r requirements.txt # Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) -COPY workers/data_refinery_workers/processors/requirements.txt . - -RUN pip3 install -r requirements.txt - -# Install this one here instead of via requirements.txt because not -# all processors need it. -RUN pip3 install rpy2==3.4.5 +# Clear out the pip3 cache. +RUN rm -rf /root/.cache ARG SYSTEM_VERSION @@ -89,6 +83,9 @@ ENV SYSTEM_VERSION $SYSTEM_VERSION USER user +COPY .boto .boto +COPY config/ config/ COPY workers/ . +COPY workers/install_ensg_pkgs.R . ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.affymetrix_local b/workers/dockerfiles/Dockerfile.affymetrix_local index 84309b4e9..9a37692e6 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix_local +++ b/workers/dockerfiles/Dockerfile.affymetrix_local @@ -6,9 +6,8 @@ USER root RUN rm -r common/ RUN pip3 uninstall -y data_refinery_common -# Reinstall common. -COPY common/dist/data-refinery-common-* common/ # Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) ARG SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 3a6df3f5f..2c6a38784 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -1,55 +1,67 @@ -FROM nvidia/cuda:11.1-runtime-ubuntu18.04 +FROM nvidia/cuda:11.8.0-runtime-ubuntu18.04 # This is very similar to the `smasher` image, but comes with OpenBLAS and some # of the other libraries required for fancyimpute. -RUN apt-get update -qq -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN apt-get update -qq +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG C.UTF-8 + +# RUN apt-key adv --fetch-keys https://developer.download.nvidia.com/compute/cuda/repos/ubuntu1804/x86_64/3bf863cc.pub # via https://github.com/ilikenwf/apt-fast/issues/85#issuecomment-261640099 RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections RUN echo debconf apt-fast/dlflag boolean true | debconf-set-selections RUN echo debconf apt-fast/aptmanager string apt-get | debconf-set-selections -RUN _APTMGR=apt-get apt-get install -y apt-fast - -RUN export DEBIAN_FRONTEND=noninteractive; \ - export DEBCONF_NONINTERACTIVE_SEEN=true; \ - echo 'tzdata tzdata/Areas select Etc' | debconf-set-selections; \ - echo 'tzdata tzdata/Zones/Etc select UTC' | debconf-set-selections; \ - apt-get update -qqy \ - && apt-get install -qqy --no-install-recommends \ - tzdata \ - && apt-get clean \ - && rm -rf /var/lib/apt/lists/* +RUN echo 'tzdata tzdata/Areas select Etc' | debconf-set-selections +RUN echo 'tzdata tzdata/Zones/Etc select UTC' | debconf-set-selections + +RUN apt-get update -qq +RUN apt-get install -y software-properties-common +RUN add-apt-repository ppa:apt-fast/stable +RUN add-apt-repository ppa:deadsnakes/ppa +RUN add-apt-repository ppa:savoury1/llvm-defaults-10 + +RUN apt-get update -qq +RUN apt-get install -y apt-fast apt-transport-https tzdata COPY workers/CRAN.gpg . -RUN \ - apt-fast update -qq && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq && \ - apt-fast install -y \ +RUN apt-key add CRAN.gpg +RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list + +RUN apt-fast update -qq && apt-fast install -y \ + build-essential \ + cmake \ + curl \ + cython3 \ ed \ + gfortran \ git \ - liblapack-dev \ - libopenblas-dev \ - python3 \ - python3-pip \ + libcairo-dev \ libcurl4-openssl-dev \ + libedit-dev \ + libblas-dev \ + liblapack-dev \ libpq-dev \ - r-base-core \ - r-base-dev \ - libpq-dev \ - libxml2-dev \ libssl-dev \ - libcurl4-openssl-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* + libxml2-dev \ + llvm-10-dev \ + lsb-release \ + mercurial \ + pkg-config \ + python3-pip \ + python3.8 \ + python3.8-dev \ + r-base-core \ + wget + RUN rm CRAN.gpg +RUN apt-get clean; rm -rf /var/lib/apt/lists/* +RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config +RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user @@ -62,7 +74,6 @@ RUN ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs /usr/local ENV R_LIBS "/usr/local/lib/R/site-library" COPY common/install_devtools.R . - RUN Rscript install_devtools.R COPY workers/R_dependencies/qn/dependencies.R . @@ -72,30 +83,21 @@ COPY workers/qn_dependencies.R . RUN Rscript qn_dependencies.R # End QN-specific -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - RUN pip3 install --upgrade pip - # Smasher-specific requirements -RUN pip3 install numpy scipy matplotlib pandas==0.25.3 scikit-learn sympy nose rpy2===3.4.5 tzlocal fancySVD +RUN pip3 install --ignore-installed numpy scipy matplotlib pandas==0.25.3 scikit-learn sympy nose rpy2===3.4.5 tzlocal fancySVD # End smasher-specific -COPY config/ config/ -COPY .boto .boto - COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed -r requirements.txt -RUN pip3 --no-cache-dir install -r requirements.txt -RUN pip3 install numpy==1.16.0 # Fix a downgrade - -COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed numpy==1.16.0 # Fix a downgrade # Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) -# Clear our the pip3 cache +# Clear out the pip3 cache. RUN rm -rf /root/.cache ARG SYSTEM_VERSION @@ -104,6 +106,8 @@ ENV SYSTEM_VERSION $SYSTEM_VERSION USER user +COPY .boto .boto +COPY config/ config/ COPY workers/ . ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index f2fc9e78a..1b3337325 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -1,59 +1,64 @@ FROM ubuntu:18.04 -RUN apt-get update -qq +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG C.UTF-8 + +RUN apt-get update RUN apt-get install -y software-properties-common RUN add-apt-repository ppa:apt-fast/stable -RUN apt-get update -qq -RUN apt-get -y install apt-fast +RUN add-apt-repository ppa:deadsnakes/ppa +RUN add-apt-repository ppa:savoury1/llvm-defaults-10 -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive +RUN apt-get update -qq +RUN apt-get install -y apt-fast apt-transport-https # The packages related to R are somewhat weird, see the README for more details. - COPY workers/CRAN.gpg . -RUN \ - apt-fast update -qq && \ - apt-get install -y apt-transport-https && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq && \ - apt-fast install -y \ +RUN apt-key add CRAN.gpg +RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list + +RUN apt-fast update -qq && apt-fast install -y \ + build-essential \ + cmake \ + curl \ + cython3 \ ed \ git \ - python3 \ - python3-pip \ - r-base-core \ - r-base-dev \ - libpq-dev \ - libxml2-dev \ - libssl-dev \ + libcairo-dev \ libcurl4-openssl-dev \ + libedit-dev \ libpq-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* + libssl-dev \ + libxml2-dev \ + llvm-10-dev \ + lsb-release \ + mercurial \ + pkg-config \ + python3-pip \ + python3.8 \ + python3.8-dev \ + r-base-core \ + wget + RUN rm CRAN.gpg +RUN apt-get clean; rm -rf /var/lib/apt/lists/* +RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config +RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -RUN pip3 install --upgrade pip - ENV R_LIBS "/usr/local/lib/R/site-library" COPY common/install_devtools.R . - RUN Rscript install_devtools.R COPY workers/install_downloader_R_only.R . - RUN Rscript install_downloader_R_only.R # Aspera will only install as the current user. @@ -71,22 +76,19 @@ RUN rm aspera-cli-3.9.1-0.tar.bz2 # Now that we're done installing Aspera go back to being root for a bit. USER root -COPY config config -COPY .boto .boto - -COPY workers/data_refinery_workers/downloaders/requirements.txt . - -RUN pip3 install -r requirements.txt - +RUN pip3 install --upgrade pip # Install this rpy2 here instead of via requirements.txt because # pip-compile throws an error for it. RUN pip3 install rpy2==3.4.5 -COPY common/dist/data-refinery-common-* common/ + +COPY workers/data_refinery_workers/downloaders/requirements.txt . +RUN pip3 install --ignore-installed -r requirements.txt # Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) -# Clear our the pip3 cache +# Clear out the pip3 cache. RUN rm -rf /root/.cache ARG SYSTEM_VERSION @@ -95,6 +97,8 @@ ENV SYSTEM_VERSION $SYSTEM_VERSION USER user +COPY .boto .boto +COPY config config COPY workers/ . ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index bc2294425..e4cc70268 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -1,46 +1,57 @@ FROM ubuntu:18.04 -RUN apt-get update -qq +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG C.UTF-8 + +RUN apt-get update RUN apt-get install -y software-properties-common RUN add-apt-repository ppa:apt-fast/stable +RUN add-apt-repository ppa:deadsnakes/ppa +RUN add-apt-repository ppa:savoury1/llvm-defaults-10 + RUN apt-get update -qq -RUN apt-get -y install apt-fast +RUN apt-get install -y apt-fast apt-transport-https # The packages related to R are somewhat weird, see the README for more details. - COPY workers/CRAN.gpg . -RUN \ - apt-fast update -qq && \ - apt-get install -y apt-transport-https && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq +RUN apt-key add CRAN.gpg +RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - -RUN apt-fast install -y \ +RUN apt-fast update -qq && apt-fast install -y \ + build-essential \ + cmake \ + curl \ + cython3 \ ed \ + gfortran \ git \ - mercurial \ libcairo-dev \ + libcurl4-openssl-dev \ libedit-dev \ + libblas-dev \ + liblapack-dev \ + libpq-dev \ + libssl-dev \ + libxml2-dev \ + llvm-10-dev \ lsb-release \ - python3 \ + mercurial \ + pkg-config \ python3-pip \ + python3.8 \ + python3.8-dev \ r-base-core \ - r-base-dev \ - libpq-dev \ - libxml2-dev \ - libssl-dev \ - libcurl4-openssl-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* + wget RUN rm CRAN.gpg +RUN apt-get clean; rm -rf /var/lib/apt/lists/* +RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config +RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user @@ -48,37 +59,24 @@ WORKDIR /home/user ENV R_LIBS "/usr/local/lib/R/site-library" COPY common/install_devtools.R . - RUN Rscript install_devtools.R COPY workers/R_dependencies/illumina/dependencies.R . RUN Rscript dependencies.R -# These are for Illumina +# These are for Illumina. COPY workers/illumina_dependencies.R . RUN Rscript illumina_dependencies.R -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - RUN pip3 install --upgrade pip - -COPY config/ config/ -COPY .boto .boto - -COPY workers/illumina_probe_maps/ probe_maps/ - COPY workers/data_refinery_workers/processors/requirements.txt . - -RUN pip3 install -r requirements.txt - -COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed -r requirements.txt # Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) -# Clear our the pip3 cache +# Clear out the pip3 cache. RUN rm -rf /root/.cache ARG SYSTEM_VERSION @@ -87,7 +85,10 @@ ENV SYSTEM_VERSION $SYSTEM_VERSION USER user -COPY workers/data_refinery_workers/processors/detect_database.R . +COPY .boto .boto +COPY config/ config/ COPY workers/ . +COPY workers/data_refinery_workers/processors/detect_database.R . +COPY workers/illumina_probe_maps/ probe_maps/ ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index ee59a63a5..98f35d772 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -1,51 +1,56 @@ FROM ubuntu:18.04 +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG C.UTF-8 + RUN apt-get update -qq RUN apt-get install -y software-properties-common + RUN add-apt-repository ppa:apt-fast/stable # deadsnakes packages new python versions for older Ubuntu releases RUN add-apt-repository ppa:deadsnakes/ppa + RUN apt-get update -qq RUN apt-get -y install apt-fast -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - # The packages related to R are somewhat weird, see the README for more details. - COPY workers/CRAN.gpg . -RUN \ - apt-fast update -qq && \ - apt-get install -y apt-transport-https && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq && \ - apt-fast install -y \ +RUN apt-key add CRAN.gpg +RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list + +RUN apt-fast update -qq && apt-fast install -y \ + build-essential \ + cmake \ + curl \ + cython3 \ ed \ git \ - mercurial \ libcairo-dev \ + libcurl4-openssl-dev \ libedit-dev \ + libfreetype6-dev \ + libpq-dev \ + libssl-dev \ + libxml2-dev \ + llvm-10-dev \ lsb-release \ - python3.6 \ - python3.6-dev \ + mercurial \ + pkg-config \ python3-pip \ + python3.8 \ + python3.8-dev \ r-base-core \ - r-base-dev \ - libpq-dev \ - libxml2-dev \ - libssl-dev \ - libcurl4-openssl-dev \ - curl \ - unzip \ - wget && \ - rm -rf /var/lib/apt/lists/* -RUN rm CRAN.gpg + wget -# Set the system python version to python3.6 from deadsnakes -RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.6 1 +RUN rm CRAN.gpg +RUN apt-get clean; rm -rf /var/lib/apt/lists/* +RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config +RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user @@ -54,7 +59,6 @@ WORKDIR /home/user ENV R_LIBS "/usr/local/lib/R/site-library" COPY common/install_devtools.R . - RUN Rscript install_devtools.R COPY workers/R_dependencies/no_op/dependencies.R . @@ -62,6 +66,7 @@ RUN Rscript dependencies.R COPY workers/install_gene_convert.R . RUN Rscript install_gene_convert.R + RUN mkdir -p gene_indexes WORKDIR /home/user/gene_indexes ENV ID_REFINERY_URL https://zenodo.org/record/1410647/files/all_1536267482.zip @@ -72,23 +77,17 @@ RUN rm *.zip WORKDIR /home/user # End Noop-specific -COPY workers/data_refinery_workers/processors/requirements.txt . - -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - RUN pip3 install --upgrade pip -RUN pip3 install -r requirements.txt +RUN pip3 install numpy -COPY config/ config/ -COPY .boto .boto -COPY common/dist/data-refinery-common-* common/ +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed -r requirements.txt # Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) -# Clear our the pip3 cache +# Clear out the pip3 cache. RUN rm -rf /root/.cache ARG SYSTEM_VERSION @@ -97,6 +96,8 @@ ENV SYSTEM_VERSION $SYSTEM_VERSION USER user +COPY .boto .boto +COPY config/ config/ COPY workers/ . ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index 31922880e..8bfbbe9a7 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -1,44 +1,54 @@ FROM ubuntu:18.04 -RUN apt-get update -qq +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG C.UTF-8 + +RUN apt-get update RUN apt-get install -y software-properties-common RUN add-apt-repository ppa:apt-fast/stable -RUN apt-get update -qq -RUN apt-get -y install apt-fast +RUN add-apt-repository ppa:deadsnakes/ppa +RUN add-apt-repository ppa:savoury1/llvm-defaults-10 -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive +RUN apt-get update -qq +RUN apt-get install -y apt-fast apt-transport-https # The packages related to R are somewhat weird, see the README for more details. - COPY workers/CRAN.gpg . +RUN apt-key add CRAN.gpg +RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list -RUN \ - apt-fast update -qq && \ - apt-get install -y apt-transport-https && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq && \ - apt-fast install -y \ +RUN apt-fast update -qq && apt-fast install -y \ + build-essential \ + cmake \ + curl \ + cython3 \ ed \ git \ - mercurial \ libcairo-dev \ + libcurl4-openssl-dev \ libedit-dev \ + libpq-dev \ + libssl-dev \ + libxml2-dev \ + llvm-10-dev \ lsb-release \ - python3 \ + mercurial \ + pkg-config \ python3-pip \ - libxml2-dev \ - cmake \ + python3.8 \ + python3.8-dev \ r-base-core \ - libssl-dev \ - libcurl4-openssl-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* + wget + RUN rm CRAN.gpg +RUN apt-get upgrade; apt-get clean; rm -rf /var/lib/apt/lists/* +RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config +RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user @@ -60,44 +70,40 @@ RUN ln -sf `pwd`/Salmon-${SALMON_VERSION}_linux_x86_64/bin/salmon /usr/local/bin RUN rm -f Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz # End Salmon installation. -# Install R dependencies +# Install R dependencies. COPY common/install_devtools.R . RUN Rscript install_devtools.R + COPY workers/R_dependencies/tximport/dependencies.R tximport_dependencies.R RUN Rscript tximport_dependencies.R -# Install tximport +# Install tximport. COPY workers/install_tximport.R . RUN Rscript install_tximport.R -# Install SalmonTools +RUN pip3 install --upgrade pip +RUN pip3 install numpy + +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed -r requirements.txt + +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) + +# Install SalmonTools. RUN git clone https://github.com/COMBINE-lab/SalmonTools.git && cd SalmonTools && git checkout 3e6654c2c10a5225498b623056993947fa688afc RUN cd SalmonTools && cmake . -DCMAKE_INSTALL_PREFIX=/usr/local && make install RUN rm -rf SalmonTools -# Install sra-tools +# Install sra-tools. ENV SRA_VERSION 2.9.1 -RUN wget "http://ftp-trace.ncbi.nlm.nih.gov/sra/sdk/${SRA_VERSION}/sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ +RUN wget "https://ftp.ncbi.nlm.nih.gov/sra/sdk/${SRA_VERSION}/sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ tar zxfv sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz && \ cp -r sratoolkit.${SRA_VERSION}-ubuntu64/bin/* /usr/bin -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -RUN pip3 install --upgrade pip - -COPY config/ config/ -COPY .boto .boto - -COPY workers/data_refinery_workers/processors/requirements.txt . - -RUN pip3 install -r requirements.txt - -COPY common/dist/data-refinery-common-* common/ - -# Get the latest version from the dist directory. -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +# Clear out the pip3 cache. +RUN rm -rf /root/.cache ARG SYSTEM_VERSION @@ -105,6 +111,8 @@ ENV SYSTEM_VERSION $SYSTEM_VERSION USER user +COPY .boto .boto +COPY config/ config/ COPY workers/ . ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index 7b95644db..313ba2150 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -1,42 +1,57 @@ FROM ubuntu:18.04 -RUN apt-get update -qq +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG C.UTF-8 + +RUN apt-get update RUN apt-get install -y software-properties-common RUN add-apt-repository ppa:apt-fast/stable -RUN apt-get update -qq -RUN apt-get -y install apt-fast +RUN add-apt-repository ppa:deadsnakes/ppa +RUN add-apt-repository ppa:savoury1/llvm-defaults-10 -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive +RUN apt-get update -qq +RUN apt-get install -y apt-fast apt-transport-https # The packages related to R are somewhat weird, see the README for more details. - COPY workers/CRAN.gpg . -RUN \ - apt-fast update -qq && \ - apt-get install -y apt-transport-https && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq && \ - apt-fast install -y \ +RUN apt-key add CRAN.gpg +RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list + +RUN apt-fast update -qq && apt-fast install -y \ + build-essential \ + cmake \ + curl \ + cython3 \ ed \ + gfortran \ git \ - python3 \ - python3-pip \ + libcairo-dev \ libcurl4-openssl-dev \ + libedit-dev \ + libblas-dev \ + liblapack-dev \ libpq-dev \ - r-base-core \ - r-base-dev \ - libpq-dev \ - libxml2-dev \ libssl-dev \ - libcurl4-openssl-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* + libxml2-dev \ + llvm-10-dev \ + lsb-release \ + mercurial \ + pkg-config \ + python3-pip \ + python3.8 \ + python3.8-dev \ + r-base-core \ + wget + RUN rm CRAN.gpg +RUN apt-get clean; rm -rf /var/lib/apt/lists/* +RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config +RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user @@ -45,7 +60,6 @@ WORKDIR /home/user ENV R_LIBS "/usr/local/lib/R/site-library" COPY common/install_devtools.R . - RUN Rscript install_devtools.R COPY workers/R_dependencies/qn/dependencies.R . @@ -55,29 +69,19 @@ COPY workers/qn_dependencies.R . RUN Rscript qn_dependencies.R # End QN-specific -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - RUN pip3 install --upgrade pip - # Smasher-specific requirements -RUN pip3 install numpy scipy matplotlib pandas==0.25.3 scikit-learn sympy nose rpy2==3.4.5 tzlocal +RUN pip3 install --ignore-installed nose numpy rpy2==3.4.5 # End smasher-specific -COPY config/ config/ -COPY .boto .boto - COPY workers/data_refinery_workers/processors/requirements.txt . - -RUN pip3 install -r requirements.txt - -COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed -r requirements.txt # Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) -# Clear our the pip3 cache +# Clear out the pip3 cache. RUN rm -rf /root/.cache ARG SYSTEM_VERSION @@ -86,6 +90,8 @@ ENV SYSTEM_VERSION $SYSTEM_VERSION USER user +COPY .boto .boto +COPY config/ config/ COPY workers/ . ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index 5cb65a6b2..d1ac0ea63 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -1,36 +1,49 @@ FROM ubuntu:18.04 +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG C.UTF-8 + RUN apt-get update -qq RUN apt-get install -y software-properties-common RUN add-apt-repository ppa:apt-fast/stable +RUN add-apt-repository ppa:deadsnakes/ppa +RUN add-apt-repository ppa:savoury1/llvm-defaults-10 + RUN apt-get update -qq RUN apt-get -y install apt-fast -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - -RUN \ - apt-fast update -qq && \ - apt-fast install -y \ +RUN apt-fast update -qq && apt-fast install -y \ + build-essential \ + curl \ + cython3 \ ed \ git \ - python3 \ - python3-pip \ libcurl4-openssl-dev \ + libfreetype6-dev \ libpq-dev \ - zlib1g-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* + llvm-10-dev \ + pkg-config \ + python3-pip \ + python3.8 \ + python3.8-dev \ + wget \ + zlib1g-dev + +RUN rm CRAN.gpg +RUN apt-get clean; rm -rf /var/lib/apt/lists/* +RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config +RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user # It's annoying that this can only be installed via git. RUN git clone https://github.com/deweylab/RSEM.git - RUN cd RSEM && make install - RUN rm -rf RSEM # Install Salmon @@ -48,36 +61,26 @@ ENV SALMON_VERSION 0.13.1 # ENV SALMON_VERSION 0.10.2 RUN wget https://github.com/COMBINE-lab/salmon/releases/download/v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz - RUN tar -xzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz # Salmon can extract to a different directory than the name of the tar file. RUN cp `tar -tzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz | head -1 | cut -f1 -d"/"`/bin/salmon /usr/local/bin - RUN cp `tar -tzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz | head -1 | cut -f1 -d"/"`/lib/* /usr/local/lib RUN rm -r Salmon* # End Salmon installation. -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - RUN pip3 install --upgrade pip - -COPY config/ config/ -COPY .boto .boto +RUN pip3 install numpy COPY workers/data_refinery_workers/processors/requirements.txt . - -RUN pip3 install -r requirements.txt - -COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed -r requirements.txt # Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) -# Clear our the pip3 cache +# Clear out the pip3 cache. RUN rm -rf /root/.cache ARG SYSTEM_VERSION @@ -86,6 +89,8 @@ ENV SYSTEM_VERSION $SYSTEM_VERSION USER user +COPY .boto .boto +COPY config/ config/ COPY workers/ . ENTRYPOINT [] From 46dfdae0bc77c8a6d5e66eba4844ab192c3bad45 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 1 Sep 2022 11:03:18 -0700 Subject: [PATCH 038/220] Add `Accession` model. Update pre-commit config. --- .pre-commit-config.yaml | 2 +- .../migrations/0071_auto_20220901_1653.py | 44 +++++++++++++++++++ .../data_refinery_common/models/__init__.py | 1 + .../data_refinery_common/models/accession.py | 22 ++++++++++ 4 files changed, 68 insertions(+), 1 deletion(-) create mode 100644 common/data_refinery_common/migrations/0071_auto_20220901_1653.py create mode 100644 common/data_refinery_common/models/accession.py diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index d024704da..b651ce24a 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -22,7 +22,7 @@ repos: - id: isort - repo: https://github.com/psf/black - rev: 19.10b0 + rev: 22.3.0 hooks: - id: black args: [--line-length=100] diff --git a/common/data_refinery_common/migrations/0071_auto_20220901_1653.py b/common/data_refinery_common/migrations/0071_auto_20220901_1653.py new file mode 100644 index 000000000..c7d3b0b63 --- /dev/null +++ b/common/data_refinery_common/migrations/0071_auto_20220901_1653.py @@ -0,0 +1,44 @@ +# Generated by Django 3.2.7 on 2022-09-01 16:53 + +from django.db import migrations, models + + +class Migration(migrations.Migration): + + dependencies = [ + ("data_refinery_common", "0070_auto_20211208_2118"), + ] + + operations = [ + migrations.CreateModel( + name="Accession", + fields=[ + ( + "id", + models.AutoField( + auto_created=True, + primary_key=True, + serialize=False, + verbose_name="ID", + ), + ), + ("code", models.TextField()), + ("created_at", models.DateTimeField(auto_now_add=True)), + ("last_modified_at", models.DateTimeField(auto_now=True)), + ("organism", models.TextField()), + ("published_date", models.DateTimeField()), + ("sample_count", models.PositiveIntegerField(default=0)), + ("source", models.TextField()), + ("technology", models.TextField()), + ], + options={ + "db_table": "accessions", + }, + ), + migrations.AddConstraint( + model_name="accession", + constraint=models.UniqueConstraint( + fields=("code", "source", "technology"), name="unique_accession" + ), + ), + ] diff --git a/common/data_refinery_common/models/__init__.py b/common/data_refinery_common/models/__init__.py index 39abe7ee3..8e9564153 100644 --- a/common/data_refinery_common/models/__init__.py +++ b/common/data_refinery_common/models/__init__.py @@ -1,3 +1,4 @@ +from data_refinery_common.models.accession import Accession # noqa from data_refinery_common.models.api_token import APIToken # noqa from data_refinery_common.models.associations.compendium_result_organism_association import ( # noqa CompendiumResultOrganismAssociation, diff --git a/common/data_refinery_common/models/accession.py b/common/data_refinery_common/models/accession.py new file mode 100644 index 000000000..dc93cfd88 --- /dev/null +++ b/common/data_refinery_common/models/accession.py @@ -0,0 +1,22 @@ +from django.db import models + + +class Accession(models.Model): + """Accession model.""" + + class Meta: + constraints = ( + models.UniqueConstraint( + fields=("code", "source", "technology"), name="unique_accession" + ), + ) + db_table = "accessions" + + code = models.TextField() + created_at = models.DateTimeField(auto_now_add=True) + last_modified_at = models.DateTimeField(auto_now=True) + organism = models.TextField() + published_date = models.DateTimeField() + sample_count = models.PositiveIntegerField(default=0) + source = models.TextField() + technology = models.TextField() From cb9249981fb8081d4bc5cf041598b17f77d83a55 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 8 Sep 2022 18:12:39 -0700 Subject: [PATCH 039/220] Port Python script to Django command. - Introduce AccessionBacklogEntry model. - Clean up command flags. - Get previous accessions from the DB. - --- .../migrations/0071_accessionbacklogentry.py | 38 + .../data_refinery_common/models/accession.py | 82 +- .../gatherer/__init__.py | 0 .../gatherer/management/__init__.py | 0 .../gatherer/management/commands/__init__.py | 0 .../management/commands/gather_accessions.py | 731 ++++++++++++++++++ foreman/data_refinery_foreman/settings.py | 17 +- foreman/dockerfiles/Dockerfile.foreman | 2 + 8 files changed, 856 insertions(+), 14 deletions(-) create mode 100644 common/data_refinery_common/migrations/0071_accessionbacklogentry.py create mode 100644 foreman/data_refinery_foreman/gatherer/__init__.py create mode 100644 foreman/data_refinery_foreman/gatherer/management/__init__.py create mode 100644 foreman/data_refinery_foreman/gatherer/management/commands/__init__.py create mode 100644 foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py diff --git a/common/data_refinery_common/migrations/0071_accessionbacklogentry.py b/common/data_refinery_common/migrations/0071_accessionbacklogentry.py new file mode 100644 index 000000000..86c04daed --- /dev/null +++ b/common/data_refinery_common/migrations/0071_accessionbacklogentry.py @@ -0,0 +1,38 @@ +# Generated by Django 3.2.7 on 2022-09-07 19:31 + +from django.db import migrations, models + + +class Migration(migrations.Migration): + + dependencies = [ + ("data_refinery_common", "0070_auto_20211208_2118"), + ] + + operations = [ + migrations.CreateModel( + name="AccessionBacklogEntry", + fields=[ + ( + "id", + models.AutoField( + auto_created=True, + primary_key=True, + serialize=False, + verbose_name="ID", + ), + ), + ("code", models.TextField(unique=True)), + ("created_at", models.DateTimeField(auto_now_add=True)), + ("last_modified_at", models.DateTimeField(auto_now=True)), + ("organism", models.TextField()), + ("published_date", models.DateTimeField()), + ("sample_count", models.PositiveIntegerField(default=0)), + ("source", models.TextField()), + ("technology", models.TextField()), + ], + options={ + "db_table": "accession_backlog", + }, + ), + ] diff --git a/common/data_refinery_common/models/accession.py b/common/data_refinery_common/models/accession.py index dc93cfd88..6ac62da9f 100644 --- a/common/data_refinery_common/models/accession.py +++ b/common/data_refinery_common/models/accession.py @@ -1,18 +1,16 @@ +from datetime import datetime + from django.db import models +from django.utils import timezone -class Accession(models.Model): - """Accession model.""" +class AccessionBacklogEntry(models.Model): + """Accession backlog entry model.""" class Meta: - constraints = ( - models.UniqueConstraint( - fields=("code", "source", "technology"), name="unique_accession" - ), - ) - db_table = "accessions" - - code = models.TextField() + db_table = "accession_backlog" + + code = models.TextField(unique=True) created_at = models.DateTimeField(auto_now_add=True) last_modified_at = models.DateTimeField(auto_now=True) organism = models.TextField() @@ -20,3 +18,67 @@ class Meta: sample_count = models.PositiveIntegerField(default=0) source = models.TextField() technology = models.TextField() + + def __eq__(self, other: object) -> bool: + """Returns True if two objects are equal. Otherwise returns False.""" + return isinstance(other, AccessionBacklogEntry) and self.code == other.code + + def __hash__(self) -> int: + """Returns accession object unique hash value.""" + return hash(self.code) + + def __str__(self) -> str: + """Returns accession default string representation.""" + return ", ".join((self.code, self.technology, self.source, str(self.published_date.date()))) + + @staticmethod + def create_from_ma_ae_entry(entry): + """Creates accession object from MicroArray ArrayExpress entry.""" + accession = AccessionBacklogEntry() + accession.code = entry["accession"] + accession.source = "ebi_biostudies" + accession.technology = "microarray" + + if "organism" in entry: + accession.organism = entry["organism"] + if "release_date" in entry: + accession.published_date = timezone.make_aware( + datetime.strptime(entry["release_date"], "%Y-%m-%d") + ) + + return accession + + @staticmethod + def create_from_ma_geo_entry(entry): + """Creates accession object from MicroArray GEO meta DB entry.""" + accession = AccessionBacklogEntry() + accession.code = entry["gse"] + accession.source = "geo_meta_db" + accession.technology = "microarray" + + if "organism" in entry: + accession.organism = entry["organism"].lower() + if "submission_date" in entry: + + accession.published_date = timezone.make_aware( + datetime.strptime(entry["submission_date"], "%Y-%m-%d") + ) + + return accession + + @staticmethod + def create_from_rnaseq_entry(entry): + """Creates accession object from RNA-Seq entry.""" + accession = AccessionBacklogEntry() + accession.code = entry["secondary_study_accession"] + accession.source = "ebi_ena_portal" + accession.technology = "rna-seq" + + if "scientific_name" in entry: + accession.organism = entry["scientific_name"].lower() + if "first_public" in entry: + accession.published_date = timezone.make_aware( + datetime.strptime(entry["first_public"], "%Y-%m-%d") + ) + + return accession diff --git a/foreman/data_refinery_foreman/gatherer/__init__.py b/foreman/data_refinery_foreman/gatherer/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/gatherer/management/__init__.py b/foreman/data_refinery_foreman/gatherer/management/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/__init__.py b/foreman/data_refinery_foreman/gatherer/management/commands/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py new file mode 100644 index 000000000..c4808a191 --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -0,0 +1,731 @@ +"""MicroArray (ArrayExpress, GEO) and RNA-Seq accession gathering automation. +Data sources: + - https://www.ebi.ac.uk/biostudies/help (MicroArray ArrayExpress). + - local SQLite meta DB from https://www.bioconductor.org/packages/release/bioc/html/GEOmetadb.html + (MicroArray GEO). + - https://www.ebi.ac.uk/ena/portal/api/ (RNA-Seq). +""" + +import argparse +import logging +import os +import re +import sqlite3 +from datetime import datetime +from http.client import RemoteDisconnected +from json.decoder import JSONDecodeError +from typing import List, Set +from urllib.parse import quote + +from django.core.management.base import BaseCommand + +import requests +from requests.exceptions import ConnectionError, ConnectTimeout +from retrying import retry +from urllib3.exceptions import ProtocolError + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.accession import AccessionBacklogEntry +from data_refinery_common.models.experiment import Experiment + +log = get_and_configure_logger(__name__) + + +class Command(BaseCommand): + """Creates agents and runs actual accession gathering.""" + + RE_ACCESSION = re.compile(r"(\D+)(\d+)") + RE_DATE = re.compile(r"\d{4}-\d{2}-\d{2}") + + # TODO(ark): remove after upgrade to python3.8 where parser argument + # "extend" action is directly available. + # https://docs.python.org/3.8/library/argparse.html#action + class ExtendAction(argparse.Action): + def __call__(self, parser, namespace, values, option_string=None): + items = getattr(namespace, self.dest) or [] + items.extend(values) + setattr(namespace, self.dest, items) + + def add_arguments(self, parser) -> None: + parser.register("action", "extend", Command.ExtendAction) + + parser.add_argument( + "--ae-id", + action="extend", + nargs="+", + type=str, + help="ArrayExpress ID(s) to use for filtering.", + ) + parser.add_argument( + "--ae-ids-file", + type=str, + help="Path to a file containing ArrayExpress ID(s) to use for filtering.", + ) + parser.add_argument("-c", "--count", type=int, help="Number of accessions to collect.") + parser.add_argument( + "-d", + "--dry-run", + action="store_true", + default=False, + help="Do not write the result to the database.", + ) + parser.add_argument( + "-e", + "--exclude-previous", + action="store_true", + default=True, + help="Exclude previously gathered or surveyed accessions.", + ) + parser.add_argument( + "-ne", + "--no-exclude-previous", + action="store_false", + default=False, + dest="exclude_previous", + help="Do not exclude previously gathered or surveyed accessions.", + ) + parser.add_argument( + "--gpl-id", + action="extend", + nargs="+", + type=str, + help="GEO platform ID(s) to use for filtering.", + ) + parser.add_argument( + "--gpl-ids-file", + type=str, + help="Path to a file containing GEO platform ID(s) to use for filtering.", + ) + parser.add_argument( + "-k", + "--keyword", + type=str, + help="Keyword to use for filtering.", + ) + parser.add_argument( + "-m", + "--microarray", + action="store_true", + default=False, + help="Collect MicroArray accessions.", + ) + parser.add_argument( + "-o", "--organism", type=str, help="Organism name to use for filtering." + ) + parser.add_argument( + "-r", + "--rna-seq", + action="store_true", + default=False, + help="Collect RNA-Seq accessions.", + ) + parser.add_argument( + "-s", + "--since", + type=str, + required=True, + help="Collect accessions made public on or after this date.", + ) + parser.add_argument( + "--taxon-id", + action="extend", + nargs="+", + type=int, + help="Taxon ID(s) to use for filtering.", + ) + parser.add_argument( + "--taxon-ids-file", + type=str, + help="Path to a file containing taxon ID(s) to use for filtering.", + ) + parser.add_argument( + "-u", + "--until", + type=str, + help="Collect accessions made public before or on this date.", + ) + parser.add_argument( + "-lv", + "--log-verbose", + action="store_true", + default=False, + help="Enable verbose log output.", + ) + + def set_verbosity_level(self, options) -> None: + """Configures log verbosity level.""" + if options["log_verbose"]: + log.addHandler(logging.StreamHandler()) + log.setLevel(logging.DEBUG) + else: + log.setLevel(logging.ERROR) + + def validate_args(self, options) -> None: + """Validates arguments.""" + if not options["microarray"] and not options["rna_seq"]: + exit("Either --microarray or --rna-seq must be specified.") + + errors = list() + since = options["since"] + until = options["until"] + if not self.RE_DATE.match(since): + errors.append('The -s, --since value must match "YYYY-MM-DD" format.') + if until and not self.RE_DATE.match(until): + errors.append('The -u, --until value must match "YYYY-MM-DD" format.') + if since and until and since > until: + errors.append("The -s, --since date must be earlier than -u, --until date.") + + keyword = options["keyword"] + organism = options["organism"] + if options["microarray"]: + ae_id = options["ae_id"] or options["ae_ids_file"] + gpl_id = options["gpl_id"] or options["gpl_ids_file"] + ids = ae_id or gpl_id + invalid_options_message = ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " + "ArrayExpress ID(s) [--ae-id, --ae-ids-file] / GEO platform ID(s) " + "[--gpl-id, --gpl-ids-file] must be specified." + ) + elif options["rna_seq"]: + taxon_id = options["taxon_id"] or options["taxon_ids_file"] + ids = taxon_id + invalid_options_message = ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] " + "or taxon ID(s) [--taxon-id, --taxon-ids-file] must be specified." + ) + + if len([option for option in (ids, keyword, organism) if option]) != 1: + errors.append(invalid_options_message) + + if errors: + exit("\n".join(errors)) + + def handle(self, *args, **options): + """Runs the accession gathering process.""" + self.validate_args(options) + self.set_verbosity_level(options) + + agents = list() + if options["rna_seq"]: + agents.append(RNASeqAccessionAgent(options)) + elif options["microarray"]: + if ( + options["ae_id"] + or options["ae_ids_file"] + or options["keyword"] + or options["organism"] + ): + agents.append(MicroArrayExpressAccessionAgent(options)) + if ( + options["gpl_id"] + or options["gpl_ids_file"] + or options["keyword"] + or options["organism"] + ): + agents.append(MicroArrayGEOAccessionAgent(options)) + + entries = set() + for agent in agents: + entries.update(agent.collect_data()) + + entries = sorted( # Sort the resulting list. + (entry for entry in entries if self.RE_ACCESSION.match(entry.code)), + key=lambda entry: ( + self.RE_ACCESSION.match(entry.code).group(1), + int(self.RE_ACCESSION.match(entry.code).group(2)), + ), + ) + # Limit the number of output entries. + entries = entries[: options["count"]] if options["count"] else entries + + if options["dry_run"]: + if entries: + output = "\n".join((str(entry) for entry in entries)) + else: + output = "No accessions found." + print(output) + else: + AccessionBacklogEntry.objects.bulk_create(entries) + + +class AccessionAgentBase: + "Accession agent base class." + + previous_accessions = set() + retry_params = { + "retry_on_exception": lambda e: isinstance( + e, (ConnectionError, ConnectTimeout, ProtocolError, RemoteDisconnected) + ), + "stop_max_attempt_number": 5, + "wait_exponential_multiplier": 1000, # Seconds. + "wait_exponential_max": 16000, # Seconds. + } + + def __init__(self, options) -> None: + """Populates args and values for major variables.""" + self.options = options + self.count = options["count"] + self.keyword = options["keyword"] + self.organism = options["organism"] + self.since = options["since"] + self.until = options["until"] or datetime.now().strftime("%Y-%m-%d") + + self.populate_previous_accessions() + + def build_query(self): + """Returns query/query dict depending on the accession data source.""" + raise NotImplementedError + + def collect_data(self): + """Generates resulting entry collection.""" + raise NotImplementedError + + def fetch_data(self): + """Fetches data from an external or local data source.""" + raise NotImplementedError + + def get_ids(self): + """Gets IDs for query filtering depending on the accession technology.""" + raise NotImplementedError + + def populate_previous_accessions(self) -> None: + """Populates previous accession set from a provided excluded ids file.""" + if not self.options["exclude_previous"] or self.previous_accessions: + return + + # Gathered accessions. + self.previous_accessions.update( + (entry["code"] for entry in AccessionBacklogEntry.objects.values("code")) + ) + + # Surveyed accessions. + experiments = Experiment.objects.values("accession_code", "alternate_accession_code") + self.previous_accessions.update( + (experiment["accession_code"] for experiment in experiments) + ) + self.previous_accessions.update( + (experiment["alternate_accession_code"] for experiment in experiments) + ) + + +class MicroArrayExpressAccessionAgent(AccessionAgentBase): + """ + MicroArray ArrayExpress accession gathering agent. The data is fetched from + the BioStudies database. See https://www.ebi.ac.uk/biostudies/help and + https://www.ebi.ac.uk/biostudies/arrayexpress/help#programmatic for more + information about the API endpoints. + """ + + def __init__(self, options) -> None: + super().__init__(options) + + self.data_chunk_size = 100 + self.data_url = "https://www.ebi.ac.uk/biostudies/api/v1/search" + self.ids = self.get_ids() + + def build_query(self) -> dict: + """Returns a query dict for getting array/organism specific accessions.""" + query_dict = { + "directsub": "true", + "page": 1, + "pageSize": self.data_chunk_size, + "release_date": f"[{self.since} TO {self.until}]", + "type": "study", + } + + if self.ids: + # TODO(ark): figure out better way of array filtering. + # Also make sure it's equivalent to the array filtering in this query + # https://github.com/AlexsLemonade/accession_retrieval/blob/master/experiment_accession_retrieval.R#L208 + query_dict.update({"content": ", ".join(self.ids)}) + elif self.keyword: + query_dict.update({"content": self.keyword}) + elif self.organism: + query_dict.update({"organism": f'"{self.organism}"'}) + + return query_dict + + def collect_data(self) -> Set[str]: + """Gets new accessions from EBI Biostudies API.""" + accessions = set() + + if self.ids: + message = ( + "Getting MicroArray ArrayExpress entries by " + f"ArrayExpress ID(s): {', '.join(self.ids)} for [{self.since} - {self.until}] " + "range." + ) + elif self.keyword: + message = ( + "Getting MicroArray ArrayExpress entries by " + f'"{self.keyword}" keyword for [{self.since} - {self.until}] range.' + ) + elif self.organism: + message = ( + "Getting MicroArray ArrayExpress entries by " + f'"{self.organism}" organism for [{self.since} - {self.until}] range.' + ) + else: + return accessions + + log.debug(message) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self) -> Set[str]: + """Retrieves accessions from API search endpoint.""" + + @retry(**self.retry_params) + def get_response(url, **kwargs): + """Gets response from an API endpoint.""" + return requests.get(url, **kwargs) + + accessions = set() + + is_done = False + params = self.build_query() + while not is_done: + range_start = (params["page"] - 1) * params["pageSize"] + 1 + range_end = (params["page"] - 1) * params["pageSize"] + self.data_chunk_size + log.debug(f"Processing entries {range_start} - {range_end}") + + response = get_response(self.data_url, params=params) + entries = response.json().get("hits") + if entries: + entries = ( + AccessionBacklogEntry.create_from_ma_ae_entry(entry) for entry in entries + ) + params["page"] += 1 + else: + is_done = True + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + # Quit after getting a sufficient amount of accessions. + if self.count and len(accessions) >= self.count: + is_done = True + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed ArrayExpress IDs.""" + ids = set() + + if self.options["ae_id"]: + ids.update(self.options["ae_id"]) + + if self.options["ae_ids_file"]: + with open(self.options["ae_ids_file"]) as ae_ids_file: + ids.update((ae_id.strip() for ae_id in ae_ids_file.readlines())) + + return sorted(ids) + + +class MicroArrayGEOAccessionAgent(AccessionAgentBase): + """ + MicroArray GEO accession gathering agent. The data is fetched from a local + SQLite GEO meta database. + """ + + def __init__(self, options) -> None: + super().__init__(options) + + self.db_path = "data/microarray/GEOmetadb.sqlite" + self.ids = self.get_ids() + + def build_query(self) -> str: + """Returns a query for getting GEO accessions from the local SQLite meta DB.""" + tables = [ + f"SELECT *", + "FROM gse_gpl", + "JOIN gpl ON gse_gpl.gpl=gpl.gpl", + "JOIN gse ON gse.gse=gse_gpl.gse", + "GROUP BY gse_gpl.gse", + ] + + conditions = [ + f"HAVING gse.submission_date >= '{self.since}'", + f"gse.submission_date <= '{self.until}'", + ] + + if self.ids: + gpl_ids = (f"'{gpl_id}'" for gpl_id in self.ids) + conditions.append(f"gse_gpl.gpl IN ({', '.join(gpl_ids)})") + elif self.organism: + conditions.append(f"lower(organism)='{self.organism.lower()}'") + + return f"{' '.join(tables)} {' AND '.join(conditions)}" + + def collect_data(self) -> Set[str]: + """Gets new accessions from GEO database.""" + accessions = set() + + if self.ids: + message = ( + "Getting MicroArray GEO entries by GEO platform ID(s): " + f"{', '.join(self.ids)} for [{self.since} - {self.until}] range." + ) + elif self.keyword: + message = ( + f'Getting MicroArray GEO entries by "{self.keyword}" keyword ' + f"for [{self.since} - {self.until}] range." + ) + elif self.organism: + message = ( + f'Getting MicroArray GEO entries by "{self.organism}" organism ' + f"for [{self.since} - {self.until}] range." + ) + else: + return accessions + + log.debug(message) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self) -> Set[str]: + """Retrieves accessions from the GEO meta DB.""" + + def match_keyword(row): + """ + Returns True if `row` matches `self.keyword` based regex. + Otherwise returns False. + """ + return re_keyword.match(" ".join((str(c) for c in row if c))) + + accessions = set() + + if not os.path.exists(self.db_path): + log.error("GEO meta database doesn't exist.") + return accessions + + connection = sqlite3.connect(self.db_path) + connection.row_factory = sqlite3.Row + connection.text_factory = lambda b: b.decode(errors="ignore") + entries = connection.execute(self.build_query()).fetchall() + connection.close() + + if self.keyword: + re_keyword = re.compile(f".*{self.keyword}.*", re.IGNORECASE) # Keyword regex. + entries = filter(match_keyword, entries) + + entries = ({key.lower(): entry[key] for key in entry.keys()} for entry in entries) + entries = set((AccessionBacklogEntry.create_from_ma_geo_entry(entry) for entry in entries)) + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed GEO platform IDs.""" + ids = set() + + if self.options["gpl_id"]: + ids.update(self.options["gpl_id"]) + + if self.options["gpl_ids_file"]: + with open(self.options["gpl_ids_file"]) as gpl_ids_file: + ids.update((gpl_id.strip() for gpl_id in gpl_ids_file.readlines())) + + return sorted(ids) + + +class RNASeqAccessionAgent(AccessionAgentBase): + """ + RNA-Seq accession gathering agent. The data is fetched from + The European Nucleotide Archive (ENA) Portal. + See https://www.ebi.ac.uk/ena/portal/api/ for more information about the API + endpoints. + """ + + def __init__(self, options) -> None: + super().__init__(options) + + self.data_chunk_size = 10000 + self.data_url = "https://www.ebi.ac.uk/ena/portal/api/search" + self.ids = self.get_ids() + + def build_query(self, taxon_id: str = None) -> str: + """ + Returns a query to use for getting specific taxon ID accessions. + Some special characters must remain unquoted. + """ + + AND = " AND " + OR = " OR " + instrument_models = ( + "HiSeq X Five", + "HiSeq X Ten", + "Illumina Genome Analyzer II", + "Illumina Genome Analyzer IIx", + "Illumina Genome Analyzer", + "Illumina HiScanSQ", + "Illumina HiSeq 1000", + "Illumina HiSeq 1500", + "Illumina HiSeq 2000", + "Illumina HiSeq 2500", + "Illumina HiSeq 3000", + "Illumina HiSeq 4000", + "Illumina MiSeq", + "Illumina NovaSeq 6000", + "Ion Torrent Proton", + "Ion Torrent S5 XL", + "Ion Torrent S5", + "NextSeq 500", + "NextSeq 550", + ) + + instrument_models = OR.join((f'instrument_model="{im}"' for im in instrument_models)) + conditions = [ + # Relevant date fields: collection_date, collection_date_submitted, + # first_public, last_updated. + f"first_public >= {self.since}", + f"first_public <= {self.until}", + f"({instrument_models})", + 'library_source="TRANSCRIPTOMIC"', + 'library_strategy="RNA-Seq"', + ] + + if taxon_id: + conditions.append(f"tax_eq({taxon_id})") + elif self.keyword: + search_fields = ( + "assembly_software", + "bio_material", + "center_name", + "collected_by", + "experiment_title", + "host_body_site", + "instrument_model", + "instrument_platform", + "library_name", + "project_name", + "sample_title", + "sequencing_method", + "study_title", + ) + search_fields = OR.join( + (f'{sf}="*{self.keyword}*"' for sf in search_fields) + ) # Keyword regex. + conditions.append(f"({search_fields})") + elif self.organism: + # `host`: Natural (as opposed to laboratory) host to the organism from which sample + # was obtained. + # `host_scientific_name`: Scientific name of the natural (as opposed to laboratory) + # host to the organism from which sample was obtained. + # `scientific_name` Scientific name of the organism from which the sample was derived. + # Neither `host_scientific_name` nor `scientific_name` available for search. + # https://www.ebi.ac.uk/ena/portal/api/searchFields?dataPortal=ena&format=json&result=read_study + conditions.append(f'host="{self.organism}"') + + return quote(AND.join(conditions), safe='*()-="<>/ ') # Must remain unquoted. + + def collect_data(self) -> Set[str]: + """Gets new accessions from EBI ENA API.""" + accessions = set() + + if self.ids: + log.debug( + f"Getting RNA-Seq entries by taxon ID(s): " + f"{', '.join((str(idx) for idx in self.ids))} for [{self.since} - {self.until}] range." + ) + total = len(self.ids) + for idx, taxon_id in enumerate(self.ids): + if self.count and len(accessions) >= self.count: + break + + if total > 1: + log.debug(f"Getting entries for taxon ID {taxon_id}, {idx + 1} of {total}.") + accessions.update(self.fetch_data(taxon_id=taxon_id)) + elif self.keyword: + log.debug( + f'Getting RNA-Seq entries by "{self.keyword}" keyword ' + f"for [{self.since} - {self.until}] range." + ) + accessions.update(self.fetch_data()) + elif self.organism: + log.debug( + f'Getting entries by "{self.organism}" organism ' + f"for [{self.since} - {self.until}] range." + ) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self, taxon_id=None) -> Set[str]: + """ + Retrieves accessions from API search endpoint. + The API allows to set limit to 0 (get all in one request) but we do + it in a paginated fashion with `self.data_chunk_size` as a page size. + """ + + @retry(**self.retry_params) + def get_response(url, **kwargs): + """Gets response from an API endpoint.""" + return requests.post(url, **kwargs) + + accessions = set() + + fields = [ + "first_public", + "scientific_name", + "secondary_study_accession", + ] # For DRP/ERP/SRP-prefixed accessions. + data = { + "dataPortal": "ena", + # TODO(ark): add excludeAccessions/excludeAccessionType support. + "fields": ",".join(fields), # Use "all" to get all fields. + "format": "json", + "limit": self.data_chunk_size, + "offset": 0, + "query": self.build_query(taxon_id=taxon_id), + "result": "read_study", + "sortFields": fields, + } + + is_done = False + while not is_done: + log.debug( + f"Processing entries {data['offset'] + 1} - {data['offset'] + self.data_chunk_size}" + ) + entries = () + try: + response = get_response(self.data_url, data=data) + entries = response.json() + # TODO(ark): add `organism` when -o, --organism flag is used. + entries = ( + AccessionBacklogEntry.create_from_rnaseq_entry(entry) for entry in entries + ) + except JSONDecodeError: + is_done = True + except TypeError: + log.error(f"Couldn't get data from {self.data_url}. Response: {entries}") + data["offset"] += self.data_chunk_size + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + # Quit after getting a sufficient amount of accessions. + if self.count and len(accessions) >= self.count: + is_done = True + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed taxon IDs.""" + ids = set() + + if self.options["taxon_id"]: + ids.update(self.options["taxon_id"]) + + if self.options["taxon_ids_file"]: + with open(self.options["taxon_ids_file"]) as taxon_id_file: + ids.update((taxon_id.strip() for taxon_id in taxon_id_file.readlines())) + + return sorted(ids) diff --git a/foreman/data_refinery_foreman/settings.py b/foreman/data_refinery_foreman/settings.py index 7a489facc..5fea76d71 100644 --- a/foreman/data_refinery_foreman/settings.py +++ b/foreman/data_refinery_foreman/settings.py @@ -47,6 +47,7 @@ "data_refinery_common", "data_refinery_foreman.surveyor", "data_refinery_foreman.foreman", + "data_refinery_foreman.gatherer", "raven.contrib.django.raven_compat", "computedfields", ] @@ -108,10 +109,18 @@ # https://docs.djangoproject.com/en/1.10/ref/settings/#auth-password-validators AUTH_PASSWORD_VALIDATORS = [ - {"NAME": "django.contrib.auth.password_validation.UserAttributeSimilarityValidator",}, - {"NAME": "django.contrib.auth.password_validation.MinimumLengthValidator",}, - {"NAME": "django.contrib.auth.password_validation.CommonPasswordValidator",}, - {"NAME": "django.contrib.auth.password_validation.NumericPasswordValidator",}, + { + "NAME": "django.contrib.auth.password_validation.UserAttributeSimilarityValidator", + }, + { + "NAME": "django.contrib.auth.password_validation.MinimumLengthValidator", + }, + { + "NAME": "django.contrib.auth.password_validation.CommonPasswordValidator", + }, + { + "NAME": "django.contrib.auth.password_validation.NumericPasswordValidator", + }, ] diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 8c09c6888..929ef2476 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -8,6 +8,8 @@ RUN apt-get -y install apt-fast RUN apt-fast update -qq && \ apt-fast install -y \ + gcc \ + libpq-dev \ python3 \ python3-pip From 4c7f8049506257c22d07bb96c606dd2221c1a8f7 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 13 Sep 2022 17:12:41 -0700 Subject: [PATCH 040/220] Address review comments. --- .../migrations/0071_auto_20220901_1653.py | 44 -- ...klogentry.py => 0071_gatheredaccession.py} | 6 +- .../data_refinery_common/models/__init__.py | 2 +- .../{accession.py => gathered_accession.py} | 20 +- .../gatherer/agents/__init__.py | 0 .../gatherer/agents/base.py | 79 +++ .../gatherer/agents/microarray_ae.py | 126 ++++ .../gatherer/agents/microarray_geo.py | 123 ++++ .../gatherer/agents/rna_seq.py | 204 ++++++ .../management/commands/gather_accessions.py | 643 +++--------------- 10 files changed, 626 insertions(+), 621 deletions(-) delete mode 100644 common/data_refinery_common/migrations/0071_auto_20220901_1653.py rename common/data_refinery_common/migrations/{0071_accessionbacklogentry.py => 0071_gatheredaccession.py} (88%) rename common/data_refinery_common/models/{accession.py => gathered_accession.py} (84%) create mode 100644 foreman/data_refinery_foreman/gatherer/agents/__init__.py create mode 100644 foreman/data_refinery_foreman/gatherer/agents/base.py create mode 100644 foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py create mode 100644 foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py create mode 100644 foreman/data_refinery_foreman/gatherer/agents/rna_seq.py diff --git a/common/data_refinery_common/migrations/0071_auto_20220901_1653.py b/common/data_refinery_common/migrations/0071_auto_20220901_1653.py deleted file mode 100644 index c7d3b0b63..000000000 --- a/common/data_refinery_common/migrations/0071_auto_20220901_1653.py +++ /dev/null @@ -1,44 +0,0 @@ -# Generated by Django 3.2.7 on 2022-09-01 16:53 - -from django.db import migrations, models - - -class Migration(migrations.Migration): - - dependencies = [ - ("data_refinery_common", "0070_auto_20211208_2118"), - ] - - operations = [ - migrations.CreateModel( - name="Accession", - fields=[ - ( - "id", - models.AutoField( - auto_created=True, - primary_key=True, - serialize=False, - verbose_name="ID", - ), - ), - ("code", models.TextField()), - ("created_at", models.DateTimeField(auto_now_add=True)), - ("last_modified_at", models.DateTimeField(auto_now=True)), - ("organism", models.TextField()), - ("published_date", models.DateTimeField()), - ("sample_count", models.PositiveIntegerField(default=0)), - ("source", models.TextField()), - ("technology", models.TextField()), - ], - options={ - "db_table": "accessions", - }, - ), - migrations.AddConstraint( - model_name="accession", - constraint=models.UniqueConstraint( - fields=("code", "source", "technology"), name="unique_accession" - ), - ), - ] diff --git a/common/data_refinery_common/migrations/0071_accessionbacklogentry.py b/common/data_refinery_common/migrations/0071_gatheredaccession.py similarity index 88% rename from common/data_refinery_common/migrations/0071_accessionbacklogentry.py rename to common/data_refinery_common/migrations/0071_gatheredaccession.py index 86c04daed..a1740d96e 100644 --- a/common/data_refinery_common/migrations/0071_accessionbacklogentry.py +++ b/common/data_refinery_common/migrations/0071_gatheredaccession.py @@ -1,4 +1,4 @@ -# Generated by Django 3.2.7 on 2022-09-07 19:31 +# Generated by Django 3.2.7 on 2022-09-13 18:14 from django.db import migrations, models @@ -11,7 +11,7 @@ class Migration(migrations.Migration): operations = [ migrations.CreateModel( - name="AccessionBacklogEntry", + name="GatheredAccession", fields=[ ( "id", @@ -32,7 +32,7 @@ class Migration(migrations.Migration): ("technology", models.TextField()), ], options={ - "db_table": "accession_backlog", + "db_table": "gathered_accessions", }, ), ] diff --git a/common/data_refinery_common/models/__init__.py b/common/data_refinery_common/models/__init__.py index 8e9564153..2b544765d 100644 --- a/common/data_refinery_common/models/__init__.py +++ b/common/data_refinery_common/models/__init__.py @@ -1,4 +1,3 @@ -from data_refinery_common.models.accession import Accession # noqa from data_refinery_common.models.api_token import APIToken # noqa from data_refinery_common.models.associations.compendium_result_organism_association import ( # noqa CompendiumResultOrganismAssociation, @@ -46,6 +45,7 @@ from data_refinery_common.models.dataset_annotation import DatasetAnnotation # noqa from data_refinery_common.models.experiment import Experiment # noqa from data_refinery_common.models.experiment_annotation import ExperimentAnnotation # noqa +from data_refinery_common.models.gathered_accession import GatheredAccession # noqa from data_refinery_common.models.jobs.downloader_job import DownloaderJob # noqa from data_refinery_common.models.jobs.processor_job import ProcessorJob # noqa from data_refinery_common.models.jobs.survey_job import SurveyJob # noqa diff --git a/common/data_refinery_common/models/accession.py b/common/data_refinery_common/models/gathered_accession.py similarity index 84% rename from common/data_refinery_common/models/accession.py rename to common/data_refinery_common/models/gathered_accession.py index 6ac62da9f..04b084533 100644 --- a/common/data_refinery_common/models/accession.py +++ b/common/data_refinery_common/models/gathered_accession.py @@ -4,11 +4,11 @@ from django.utils import timezone -class AccessionBacklogEntry(models.Model): - """Accession backlog entry model.""" +class GatheredAccession(models.Model): + """Gathered accession model.""" class Meta: - db_table = "accession_backlog" + db_table = "gathered_accessions" code = models.TextField(unique=True) created_at = models.DateTimeField(auto_now_add=True) @@ -21,7 +21,7 @@ class Meta: def __eq__(self, other: object) -> bool: """Returns True if two objects are equal. Otherwise returns False.""" - return isinstance(other, AccessionBacklogEntry) and self.code == other.code + return isinstance(other, GatheredAccession) and self.code == other.code def __hash__(self) -> int: """Returns accession object unique hash value.""" @@ -32,15 +32,15 @@ def __str__(self) -> str: return ", ".join((self.code, self.technology, self.source, str(self.published_date.date()))) @staticmethod - def create_from_ma_ae_entry(entry): + def create_from_ma_ae_entry(entry, organism=None): """Creates accession object from MicroArray ArrayExpress entry.""" - accession = AccessionBacklogEntry() + accession = GatheredAccession() accession.code = entry["accession"] accession.source = "ebi_biostudies" accession.technology = "microarray" - if "organism" in entry: - accession.organism = entry["organism"] + if organism: + accession.organism = organism if "release_date" in entry: accession.published_date = timezone.make_aware( datetime.strptime(entry["release_date"], "%Y-%m-%d") @@ -51,7 +51,7 @@ def create_from_ma_ae_entry(entry): @staticmethod def create_from_ma_geo_entry(entry): """Creates accession object from MicroArray GEO meta DB entry.""" - accession = AccessionBacklogEntry() + accession = GatheredAccession() accession.code = entry["gse"] accession.source = "geo_meta_db" accession.technology = "microarray" @@ -69,7 +69,7 @@ def create_from_ma_geo_entry(entry): @staticmethod def create_from_rnaseq_entry(entry): """Creates accession object from RNA-Seq entry.""" - accession = AccessionBacklogEntry() + accession = GatheredAccession() accession.code = entry["secondary_study_accession"] accession.source = "ebi_ena_portal" accession.technology = "rna-seq" diff --git a/foreman/data_refinery_foreman/gatherer/agents/__init__.py b/foreman/data_refinery_foreman/gatherer/agents/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/gatherer/agents/base.py b/foreman/data_refinery_foreman/gatherer/agents/base.py new file mode 100644 index 000000000..3754a4068 --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/agents/base.py @@ -0,0 +1,79 @@ +"""Abstract base class for accession gathering automation agents.""" + +from abc import ABC, abstractmethod +from datetime import datetime +from http.client import RemoteDisconnected + +from requests.exceptions import ConnectionError, ConnectTimeout +from urllib3.exceptions import ProtocolError + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.experiment import Experiment +from data_refinery_common.models.gathered_accession import GatheredAccession + +logger = get_and_configure_logger(__name__) + + +class AccessionAgentBase(ABC): + "Accession agent abstract base class." + + previous_accessions = set() + retry_params = { + "retry_on_exception": lambda e: isinstance( + e, (ConnectionError, ConnectTimeout, ProtocolError, RemoteDisconnected) + ), + "stop_max_attempt_number": 5, + "wait_exponential_multiplier": 1000, # Seconds. + "wait_exponential_max": 16000, # Seconds. + } + + def __init__(self, options) -> None: + """Populates args and values for major variables.""" + self.options = options + self.count = options["count"] + self.keyword = options["keyword"] + self.organism = options["organism"] + self.since = options["since"] + self.until = options["until"] or datetime.now().strftime("%Y-%m-%d") + + self.ids = self.get_ids() + self.populate_previous_accessions() + + @abstractmethod + def build_query(self): + """Returns query/query dict depending on the accession data source.""" + pass + + @abstractmethod + def collect_data(self): + """Generates resulting entry collection.""" + pass + + @abstractmethod + def fetch_data(self): + """Fetches data from an external or local data source.""" + pass + + @abstractmethod + def get_ids(self): + """Gets IDs for query filtering depending on the accession technology.""" + pass + + def populate_previous_accessions(self) -> None: + """Populates previous accession set from a provided excluded ids file.""" + if not self.options["exclude_previous"] or self.previous_accessions: + return + + # Gathered accessions. + self.previous_accessions.update( + (entry["code"] for entry in GatheredAccession.objects.values("code")) + ) + + # Surveyed accessions. + experiments = Experiment.objects.values("accession_code", "alternate_accession_code") + self.previous_accessions.update( + (experiment["accession_code"] for experiment in experiments) + ) + self.previous_accessions.update( + (experiment["alternate_accession_code"] for experiment in experiments) + ) diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py new file mode 100644 index 000000000..b5314302b --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py @@ -0,0 +1,126 @@ +"""MicroArray ArrayExpress accession gathering automation. +Data source: https://www.ebi.ac.uk/biostudies/help""" + +from typing import List, Set + +import requests +from retrying import retry + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.gathered_accession import GatheredAccession +from data_refinery_foreman.gatherer.agents.base import AccessionAgentBase + +logger = get_and_configure_logger(__name__) + + +class MicroArrayExpressAccessionAgent(AccessionAgentBase): + """ + MicroArray ArrayExpress accession gathering agent. The data is fetched from + the BioStudies database. See https://www.ebi.ac.uk/biostudies/help and + https://www.ebi.ac.uk/biostudies/arrayexpress/help#programmatic for more + information about the API endpoints. + """ + + DATA_CHUNK_SIZE = 100 + DATA_URL = "https://www.ebi.ac.uk/biostudies/api/v1/search" + + def build_query(self) -> dict: + """Returns a query dict for getting array/organism specific accessions.""" + query_dict = { + "directsub": "true", + "page": 1, + "pageSize": self.DATA_CHUNK_SIZE, + "release_date": f"[{self.since} TO {self.until}]", + "type": "study", + } + + if self.ids: + # TODO(ark): figure out better way of array filtering. + # Also make sure it's equivalent to the array filtering in this query + # https://github.com/AlexsLemonade/accession_retrieval/blob/master/experiment_accession_retrieval.R#L208 + query_dict.update({"content": ", ".join(self.ids)}) + elif self.keyword: + query_dict.update({"content": self.keyword}) + elif self.organism: + query_dict.update({"organism": f'"{self.organism}"'}) + + return query_dict + + def collect_data(self) -> Set[str]: + """Gets new accessions from EBI Biostudies API.""" + accessions = set() + + if self.ids: + message = ( + "Getting MicroArray ArrayExpress entries by " + f"ArrayExpress ID(s): {', '.join(self.ids)} for [{self.since} - {self.until}] " + "range." + ) + elif self.keyword: + message = ( + "Getting MicroArray ArrayExpress entries by " + f'"{self.keyword}" keyword for [{self.since} - {self.until}] range.' + ) + elif self.organism: + message = ( + "Getting MicroArray ArrayExpress entries by " + f'"{self.organism}" organism for [{self.since} - {self.until}] range.' + ) + else: + return accessions + + logger.debug(message) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self) -> Set[str]: + """Retrieves accessions from API search endpoint.""" + + @retry(**self.retry_params) + def get_response(url, **kwargs): + """Gets response from an API endpoint.""" + return requests.get(url, **kwargs) + + accessions = set() + + is_done = False + params = self.build_query() + while not is_done: + range_start = (params["page"] - 1) * params["pageSize"] + 1 + range_end = (params["page"] - 1) * params["pageSize"] + self.DATA_CHUNK_SIZE + logger.debug(f"Processing entries {range_start} - {range_end}") + + response = get_response(self.DATA_URL, params=params) + entries = response.json().get("hits") + if entries: + entries = ( + GatheredAccession.create_from_ma_ae_entry(entry, organism=self.organism) + for entry in entries + ) + params["page"] += 1 + else: + is_done = True + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + # Quit after getting a sufficient amount of accessions. + if self.count and len(accessions) >= self.count: + is_done = True + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed ArrayExpress IDs.""" + ids = set() + + if self.options["ae_id"]: + ids.update(self.options["ae_id"]) + + if self.options["ae_ids_file"]: + with open(self.options["ae_ids_file"]) as ae_ids_file: + ids.update((ae_id.strip() for ae_id in ae_ids_file.readlines())) + + return sorted(ids) diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py new file mode 100644 index 000000000..975c715b3 --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py @@ -0,0 +1,123 @@ +"""MicroArray GEO accession gathering automation. +Data source: local SQLite meta DB from https://www.bioconductor.org/packages/release/bioc/html/GEOmetadb.html""" + +import os +import re +import sqlite3 +from typing import List, Set + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.gathered_accession import GatheredAccession +from data_refinery_foreman.gatherer.agents.base import AccessionAgentBase + +logger = get_and_configure_logger(__name__) + + +class MicroArrayGEOAccessionAgent(AccessionAgentBase): + """ + MicroArray GEO accession gathering agent. The data is fetched from a local + SQLite GEO meta database. + """ + + # TODO(ark): move the DB file from Docker image to S3. + # Implement syncing procedure. + # Update URL once the original file is available again. + DB_PATH = "data/microarray/GEOmetadb.sqlite" + + def build_query(self) -> str: + """Returns a query for getting GEO accessions from the local SQLite meta DB.""" + tables = [ + "SELECT *", + "FROM gse_gpl", + "JOIN gpl ON gse_gpl.gpl=gpl.gpl", + "JOIN gse ON gse.gse=gse_gpl.gse", + "GROUP BY gse_gpl.gse", + ] + + conditions = [ + f"HAVING gse.submission_date >= '{self.since}'", + f"gse.submission_date <= '{self.until}'", + ] + + if self.ids: + gpl_ids = (f"'{gpl_id}'" for gpl_id in self.ids) + conditions.append(f"gse_gpl.gpl IN ({', '.join(gpl_ids)})") + elif self.organism: + conditions.append(f"lower(organism)='{self.organism.lower()}'") + + return f"{' '.join(tables)} {' AND '.join(conditions)}" + + def collect_data(self) -> Set[str]: + """Gets new accessions from GEO database.""" + accessions = set() + + if self.ids: + message = ( + "Getting MicroArray GEO entries by GEO platform ID(s): " + f"{', '.join(self.ids)} for [{self.since} - {self.until}] range." + ) + elif self.keyword: + message = ( + f'Getting MicroArray GEO entries by "{self.keyword}" keyword ' + f"for [{self.since} - {self.until}] range." + ) + elif self.organism: + message = ( + f'Getting MicroArray GEO entries by "{self.organism}" organism ' + f"for [{self.since} - {self.until}] range." + ) + else: + return accessions + + logger.debug(message) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self) -> Set[str]: + """Retrieves accessions from the GEO meta DB.""" + + def match_keyword(row): + """ + Returns True if `row` matches `self.keyword` based regex. + Otherwise returns False. + """ + return re_keyword.match(" ".join((str(c) for c in row if c))) + + accessions = set() + + if not os.path.exists(self.DB_PATH): + logger.error("GEO meta database doesn't exist.") + return accessions + + connection = sqlite3.connect(self.DB_PATH) + connection.row_factory = sqlite3.Row + connection.text_factory = lambda b: b.decode(errors="ignore") + entries = connection.execute(self.build_query()).fetchall() + connection.close() + + if self.keyword: + re_keyword = re.compile(f".*{self.keyword}.*", re.IGNORECASE) # Keyword regex. + entries = filter(match_keyword, entries) + + entries = ({key.lower(): entry[key] for key in entry.keys()} for entry in entries) + entries = set((GatheredAccession.create_from_ma_geo_entry(entry) for entry in entries)) + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed GEO platform IDs.""" + ids = set() + + if self.options["gpl_id"]: + ids.update(self.options["gpl_id"]) + + if self.options["gpl_ids_file"]: + with open(self.options["gpl_ids_file"]) as gpl_ids_file: + ids.update((gpl_id.strip() for gpl_id in gpl_ids_file.readlines())) + + return sorted(ids) diff --git a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py new file mode 100644 index 000000000..f9497f3ba --- /dev/null +++ b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py @@ -0,0 +1,204 @@ +"""RNA-Seq accession gathering automation. +Data source: https://www.ebi.ac.uk/ena/portal/api/""" + +from json.decoder import JSONDecodeError +from typing import List, Set +from urllib.parse import quote + +import requests +from retrying import retry + +from data_refinery_common.logging import get_and_configure_logger +from data_refinery_common.models.gathered_accession import GatheredAccession +from data_refinery_foreman.gatherer.agents.base import AccessionAgentBase + +logger = get_and_configure_logger(__name__) + + +class RNASeqAccessionAgent(AccessionAgentBase): + """ + RNA-Seq accession gathering agent. The data is fetched from + The European Nucleotide Archive (ENA) Portal. + See https://www.ebi.ac.uk/ena/portal/api/ for more information about the API + endpoints. + """ + + DATA_CHUNK_SIZE = 10000 + DATA_URL = "https://www.ebi.ac.uk/ena/portal/api/search" + + def build_query(self, taxon_id: str = None) -> str: + """ + Returns a query to use for getting specific taxon ID accessions. + Some special characters must remain unquoted. + """ + + AND = " AND " + OR = " OR " + instrument_models = ( + "HiSeq X Five", + "HiSeq X Ten", + "Illumina Genome Analyzer II", + "Illumina Genome Analyzer IIx", + "Illumina Genome Analyzer", + "Illumina HiScanSQ", + "Illumina HiSeq 1000", + "Illumina HiSeq 1500", + "Illumina HiSeq 2000", + "Illumina HiSeq 2500", + "Illumina HiSeq 3000", + "Illumina HiSeq 4000", + "Illumina MiSeq", + "Illumina NovaSeq 6000", + "Ion Torrent Proton", + "Ion Torrent S5 XL", + "Ion Torrent S5", + "NextSeq 500", + "NextSeq 550", + ) + + instrument_models = OR.join((f'instrument_model="{im}"' for im in instrument_models)) + conditions = [ + # Relevant date fields: collection_date, collection_date_submitted, + # first_public, last_updated. + f"first_public >= {self.since}", + f"first_public <= {self.until}", + f"({instrument_models})", + 'library_source="TRANSCRIPTOMIC"', + 'library_strategy="RNA-Seq"', + ] + + if taxon_id: + conditions.append(f"tax_eq({taxon_id})") + elif self.keyword: + search_fields = ( + "assembly_software", + "bio_material", + "center_name", + "collected_by", + "experiment_title", + "host_body_site", + "instrument_model", + "instrument_platform", + "library_name", + "project_name", + "sample_title", + "sequencing_method", + "study_title", + ) + search_fields = OR.join( + (f'{sf}="*{self.keyword}*"' for sf in search_fields) + ) # Keyword regex. + conditions.append(f"({search_fields})") + elif self.organism: + # `host`: Natural (as opposed to laboratory) host to the organism from which sample + # was obtained. + # `host_scientific_name`: Scientific name of the natural (as opposed to laboratory) + # host to the organism from which sample was obtained. + # `scientific_name` Scientific name of the organism from which the sample was derived. + # Neither `host_scientific_name` nor `scientific_name` available for search. + # https://www.ebi.ac.uk/ena/portal/api/searchFields?dataPortal=ena&format=json&result=read_study + conditions.append(f'host="{self.organism}"') + + return quote(AND.join(conditions), safe='*()-="<>/ ') # Must remain unquoted. + + def collect_data(self) -> Set[str]: + """Gets new accessions from EBI ENA API.""" + accessions = set() + + if self.ids: + logger.debug( + f"Getting RNA-Seq entries by taxon ID(s): " + f"{', '.join((str(i) for i in self.ids))} for [{self.since} - {self.until}] range." + ) + total = len(self.ids) + for idx, taxon_id in enumerate(self.ids): + if self.count and len(accessions) >= self.count: + break + + if total > 1: + logger.debug(f"Getting entries for taxon ID {taxon_id}, {idx + 1} of {total}.") + accessions.update(self.fetch_data(taxon_id=taxon_id)) + elif self.keyword: + logger.debug( + f'Getting RNA-Seq entries by "{self.keyword}" keyword ' + f"for [{self.since} - {self.until}] range." + ) + accessions.update(self.fetch_data()) + elif self.organism: + logger.debug( + f'Getting entries by "{self.organism}" organism ' + f"for [{self.since} - {self.until}] range." + ) + accessions.update(self.fetch_data()) + + return accessions + + def fetch_data(self, taxon_id=None) -> Set[str]: + """ + Retrieves accessions from API search endpoint. + The API allows to set limit to 0 (get all in one request) but we do + it in a paginated fashion with `self.DATA_CHUNK_SIZE` as a page size. + """ + + @retry(**self.retry_params) + def get_response(url, **kwargs): + """Gets response from an API endpoint.""" + return requests.post(url, **kwargs) + + accessions = set() + + fields = [ + "first_public", + "scientific_name", + "secondary_study_accession", + ] # For DRP/ERP/SRP-prefixed accessions. + data = { + "dataPortal": "ena", + # TODO(ark): add excludeAccessions/excludeAccessionType support. + "fields": ",".join(fields), # Use "all" to get all fields. + "format": "json", + "limit": self.DATA_CHUNK_SIZE, + "offset": 0, + "query": self.build_query(taxon_id=taxon_id), + "result": "read_study", + "sortFields": fields, + } + + is_done = False + while not is_done: + logger.debug( + f"Processing entries {data['offset'] + 1} - {data['offset'] + self.DATA_CHUNK_SIZE}" + ) + entries = () + try: + response = get_response(self.DATA_URL, data=data) + entries = response.json() + entries = (GatheredAccession.create_from_rnaseq_entry(entry) for entry in entries) + except JSONDecodeError: + is_done = True + except TypeError: + logger.error(f"Couldn't get data from {self.data_url}. Response: {entries}") + data["offset"] += self.DATA_CHUNK_SIZE + + if self.previous_accessions: + entries = (entry for entry in entries if entry.code not in self.previous_accessions) + accessions.update(entries) + + # Quit after getting a sufficient amount of accessions. + if self.count and len(accessions) >= self.count: + is_done = True + + return accessions + + def get_ids(self) -> List[str]: + """Returns a combined list of passed taxon IDs.""" + ids = set() + + if self.options["taxon_id"]: + ids.update(self.options["taxon_id"]) + + if self.options["taxon_ids_file"]: + with open(self.options["taxon_ids_file"]) as taxon_id_file: + ids.update((taxon_id.strip() for taxon_id in taxon_id_file.readlines())) + + return sorted(ids) diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index c4808a191..445245d3a 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -8,32 +8,27 @@ import argparse import logging -import os import re -import sqlite3 -from datetime import datetime -from http.client import RemoteDisconnected -from json.decoder import JSONDecodeError -from typing import List, Set -from urllib.parse import quote from django.core.management.base import BaseCommand -import requests -from requests.exceptions import ConnectionError, ConnectTimeout -from retrying import retry -from urllib3.exceptions import ProtocolError - from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models.accession import AccessionBacklogEntry -from data_refinery_common.models.experiment import Experiment +from data_refinery_common.models.gathered_accession import GatheredAccession +from data_refinery_foreman.gatherer.agents.microarray_ae import MicroArrayExpressAccessionAgent +from data_refinery_foreman.gatherer.agents.microarray_geo import MicroArrayGEOAccessionAgent +from data_refinery_foreman.gatherer.agents.rna_seq import RNASeqAccessionAgent -log = get_and_configure_logger(__name__) +logger = get_and_configure_logger(__name__) class Command(BaseCommand): """Creates agents and runs actual accession gathering.""" + DATA_SOURCE_MA_AE = "microarray-ae" + DATA_SOURCE_MA_GEO = "microarray-geo" + DATA_SOURCE_RNA_SEQ = "rna-seq" + DATA_SOURCES = (DATA_SOURCE_MA_AE, DATA_SOURCE_MA_GEO, DATA_SOURCE_RNA_SEQ) + RE_ACCESSION = re.compile(r"(\D+)(\d+)") RE_DATE = re.compile(r"\d{4}-\d{2}-\d{2}") @@ -76,14 +71,6 @@ def add_arguments(self, parser) -> None: default=True, help="Exclude previously gathered or surveyed accessions.", ) - parser.add_argument( - "-ne", - "--no-exclude-previous", - action="store_false", - default=False, - dest="exclude_previous", - help="Do not exclude previously gathered or surveyed accessions.", - ) parser.add_argument( "--gpl-id", action="extend", @@ -103,21 +90,22 @@ def add_arguments(self, parser) -> None: help="Keyword to use for filtering.", ) parser.add_argument( - "-m", - "--microarray", + "-lv", + "--log-verbose", action="store_true", default=False, - help="Collect MicroArray accessions.", + help="Enable verbose log output.", ) parser.add_argument( - "-o", "--organism", type=str, help="Organism name to use for filtering." + "-ne", + "--no-exclude-previous", + action="store_false", + default=False, + dest="exclude_previous", + help="Do not exclude previously gathered or surveyed accessions.", ) parser.add_argument( - "-r", - "--rna-seq", - action="store_true", - default=False, - help="Collect RNA-Seq accessions.", + "-o", "--organism", type=str, help="Organism name to use for filtering." ) parser.add_argument( "-s", @@ -126,6 +114,14 @@ def add_arguments(self, parser) -> None: required=True, help="Collect accessions made public on or after this date.", ) + parser.add_argument( + "-src", + "--source", + type=str, + action="extend", + nargs="+", + help="Gather accessions from selected sources.", + ) parser.add_argument( "--taxon-id", action="extend", @@ -144,28 +140,19 @@ def add_arguments(self, parser) -> None: type=str, help="Collect accessions made public before or on this date.", ) - parser.add_argument( - "-lv", - "--log-verbose", - action="store_true", - default=False, - help="Enable verbose log output.", - ) def set_verbosity_level(self, options) -> None: """Configures log verbosity level.""" if options["log_verbose"]: - log.addHandler(logging.StreamHandler()) - log.setLevel(logging.DEBUG) + logger.addHandler(logging.StreamHandler()) + logger.setLevel(logging.DEBUG) else: - log.setLevel(logging.ERROR) + logger.setLevel(logging.ERROR) def validate_args(self, options) -> None: """Validates arguments.""" - if not options["microarray"] and not options["rna_seq"]: - exit("Either --microarray or --rna-seq must be specified.") - errors = list() + since = options["since"] until = options["until"] if not self.RE_DATE.match(since): @@ -177,52 +164,65 @@ def validate_args(self, options) -> None: keyword = options["keyword"] organism = options["organism"] - if options["microarray"]: - ae_id = options["ae_id"] or options["ae_ids_file"] - gpl_id = options["gpl_id"] or options["gpl_ids_file"] - ids = ae_id or gpl_id - invalid_options_message = ( - "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " - "ArrayExpress ID(s) [--ae-id, --ae-ids-file] / GEO platform ID(s) " - "[--gpl-id, --gpl-ids-file] must be specified." - ) - elif options["rna_seq"]: - taxon_id = options["taxon_id"] or options["taxon_ids_file"] - ids = taxon_id - invalid_options_message = ( - "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] " - "or taxon ID(s) [--taxon-id, --taxon-ids-file] must be specified." + sources = options["source"] or self.DATA_SOURCES + + for source in sources: + if source in self.DATA_SOURCES: + continue + errors.append( + f"Unknown source: {source}. Supported sources: {', '.join(self.DATA_SOURCES)}" ) - if len([option for option in (ids, keyword, organism) if option]) != 1: - errors.append(invalid_options_message) + if self.DATA_SOURCE_MA_AE in sources: + ids = options["ae_id"] or options["ae_ids_file"] + if not (ids or keyword or organism): + errors.append( + ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " + "ArrayExpress ID(s) [--ae-id, --ae-ids-file] must be specified for " + f"'{self.DATA_SOURCE_MA_AE}' source." + ) + ) + if self.DATA_SOURCE_MA_GEO in sources: + ids = options["gpl_id"] or options["gpl_ids_file"] + if not (ids or keyword or organism): + errors.append( + ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " + "GEO platform ID(s) [--gpl-id, --gpl-ids-file] must be specified for " + f"'{self.DATA_SOURCE_MA_GEO}' source." + ) + ) + if self.DATA_SOURCE_RNA_SEQ in sources: + ids = options["taxon_id"] or options["taxon_ids_file"] + if not (ids or keyword or organism): + errors.append( + ( + "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] " + "or taxon ID(s) [--taxon-id, --taxon-ids-file] must be specified for " + f"'{self.DATA_SOURCE_RNA_SEQ}' source." + ) + ) if errors: exit("\n".join(errors)) def handle(self, *args, **options): - """Runs the accession gathering process.""" + """Creates agents and runs the accession gathering process.""" self.validate_args(options) self.set_verbosity_level(options) agents = list() - if options["rna_seq"]: + sources = options["source"] or self.DATA_SOURCES + + if self.DATA_SOURCE_RNA_SEQ in sources: agents.append(RNASeqAccessionAgent(options)) - elif options["microarray"]: - if ( - options["ae_id"] - or options["ae_ids_file"] - or options["keyword"] - or options["organism"] - ): - agents.append(MicroArrayExpressAccessionAgent(options)) - if ( - options["gpl_id"] - or options["gpl_ids_file"] - or options["keyword"] - or options["organism"] - ): - agents.append(MicroArrayGEOAccessionAgent(options)) + + if self.DATA_SOURCE_MA_AE in sources: + agents.append(MicroArrayExpressAccessionAgent(options)) + + if self.DATA_SOURCE_MA_GEO in sources: + agents.append(MicroArrayGEOAccessionAgent(options)) entries = set() for agent in agents: @@ -245,487 +245,4 @@ def handle(self, *args, **options): output = "No accessions found." print(output) else: - AccessionBacklogEntry.objects.bulk_create(entries) - - -class AccessionAgentBase: - "Accession agent base class." - - previous_accessions = set() - retry_params = { - "retry_on_exception": lambda e: isinstance( - e, (ConnectionError, ConnectTimeout, ProtocolError, RemoteDisconnected) - ), - "stop_max_attempt_number": 5, - "wait_exponential_multiplier": 1000, # Seconds. - "wait_exponential_max": 16000, # Seconds. - } - - def __init__(self, options) -> None: - """Populates args and values for major variables.""" - self.options = options - self.count = options["count"] - self.keyword = options["keyword"] - self.organism = options["organism"] - self.since = options["since"] - self.until = options["until"] or datetime.now().strftime("%Y-%m-%d") - - self.populate_previous_accessions() - - def build_query(self): - """Returns query/query dict depending on the accession data source.""" - raise NotImplementedError - - def collect_data(self): - """Generates resulting entry collection.""" - raise NotImplementedError - - def fetch_data(self): - """Fetches data from an external or local data source.""" - raise NotImplementedError - - def get_ids(self): - """Gets IDs for query filtering depending on the accession technology.""" - raise NotImplementedError - - def populate_previous_accessions(self) -> None: - """Populates previous accession set from a provided excluded ids file.""" - if not self.options["exclude_previous"] or self.previous_accessions: - return - - # Gathered accessions. - self.previous_accessions.update( - (entry["code"] for entry in AccessionBacklogEntry.objects.values("code")) - ) - - # Surveyed accessions. - experiments = Experiment.objects.values("accession_code", "alternate_accession_code") - self.previous_accessions.update( - (experiment["accession_code"] for experiment in experiments) - ) - self.previous_accessions.update( - (experiment["alternate_accession_code"] for experiment in experiments) - ) - - -class MicroArrayExpressAccessionAgent(AccessionAgentBase): - """ - MicroArray ArrayExpress accession gathering agent. The data is fetched from - the BioStudies database. See https://www.ebi.ac.uk/biostudies/help and - https://www.ebi.ac.uk/biostudies/arrayexpress/help#programmatic for more - information about the API endpoints. - """ - - def __init__(self, options) -> None: - super().__init__(options) - - self.data_chunk_size = 100 - self.data_url = "https://www.ebi.ac.uk/biostudies/api/v1/search" - self.ids = self.get_ids() - - def build_query(self) -> dict: - """Returns a query dict for getting array/organism specific accessions.""" - query_dict = { - "directsub": "true", - "page": 1, - "pageSize": self.data_chunk_size, - "release_date": f"[{self.since} TO {self.until}]", - "type": "study", - } - - if self.ids: - # TODO(ark): figure out better way of array filtering. - # Also make sure it's equivalent to the array filtering in this query - # https://github.com/AlexsLemonade/accession_retrieval/blob/master/experiment_accession_retrieval.R#L208 - query_dict.update({"content": ", ".join(self.ids)}) - elif self.keyword: - query_dict.update({"content": self.keyword}) - elif self.organism: - query_dict.update({"organism": f'"{self.organism}"'}) - - return query_dict - - def collect_data(self) -> Set[str]: - """Gets new accessions from EBI Biostudies API.""" - accessions = set() - - if self.ids: - message = ( - "Getting MicroArray ArrayExpress entries by " - f"ArrayExpress ID(s): {', '.join(self.ids)} for [{self.since} - {self.until}] " - "range." - ) - elif self.keyword: - message = ( - "Getting MicroArray ArrayExpress entries by " - f'"{self.keyword}" keyword for [{self.since} - {self.until}] range.' - ) - elif self.organism: - message = ( - "Getting MicroArray ArrayExpress entries by " - f'"{self.organism}" organism for [{self.since} - {self.until}] range.' - ) - else: - return accessions - - log.debug(message) - accessions.update(self.fetch_data()) - - return accessions - - def fetch_data(self) -> Set[str]: - """Retrieves accessions from API search endpoint.""" - - @retry(**self.retry_params) - def get_response(url, **kwargs): - """Gets response from an API endpoint.""" - return requests.get(url, **kwargs) - - accessions = set() - - is_done = False - params = self.build_query() - while not is_done: - range_start = (params["page"] - 1) * params["pageSize"] + 1 - range_end = (params["page"] - 1) * params["pageSize"] + self.data_chunk_size - log.debug(f"Processing entries {range_start} - {range_end}") - - response = get_response(self.data_url, params=params) - entries = response.json().get("hits") - if entries: - entries = ( - AccessionBacklogEntry.create_from_ma_ae_entry(entry) for entry in entries - ) - params["page"] += 1 - else: - is_done = True - - if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) - accessions.update(entries) - - # Quit after getting a sufficient amount of accessions. - if self.count and len(accessions) >= self.count: - is_done = True - - return accessions - - def get_ids(self) -> List[str]: - """Returns a combined list of passed ArrayExpress IDs.""" - ids = set() - - if self.options["ae_id"]: - ids.update(self.options["ae_id"]) - - if self.options["ae_ids_file"]: - with open(self.options["ae_ids_file"]) as ae_ids_file: - ids.update((ae_id.strip() for ae_id in ae_ids_file.readlines())) - - return sorted(ids) - - -class MicroArrayGEOAccessionAgent(AccessionAgentBase): - """ - MicroArray GEO accession gathering agent. The data is fetched from a local - SQLite GEO meta database. - """ - - def __init__(self, options) -> None: - super().__init__(options) - - self.db_path = "data/microarray/GEOmetadb.sqlite" - self.ids = self.get_ids() - - def build_query(self) -> str: - """Returns a query for getting GEO accessions from the local SQLite meta DB.""" - tables = [ - f"SELECT *", - "FROM gse_gpl", - "JOIN gpl ON gse_gpl.gpl=gpl.gpl", - "JOIN gse ON gse.gse=gse_gpl.gse", - "GROUP BY gse_gpl.gse", - ] - - conditions = [ - f"HAVING gse.submission_date >= '{self.since}'", - f"gse.submission_date <= '{self.until}'", - ] - - if self.ids: - gpl_ids = (f"'{gpl_id}'" for gpl_id in self.ids) - conditions.append(f"gse_gpl.gpl IN ({', '.join(gpl_ids)})") - elif self.organism: - conditions.append(f"lower(organism)='{self.organism.lower()}'") - - return f"{' '.join(tables)} {' AND '.join(conditions)}" - - def collect_data(self) -> Set[str]: - """Gets new accessions from GEO database.""" - accessions = set() - - if self.ids: - message = ( - "Getting MicroArray GEO entries by GEO platform ID(s): " - f"{', '.join(self.ids)} for [{self.since} - {self.until}] range." - ) - elif self.keyword: - message = ( - f'Getting MicroArray GEO entries by "{self.keyword}" keyword ' - f"for [{self.since} - {self.until}] range." - ) - elif self.organism: - message = ( - f'Getting MicroArray GEO entries by "{self.organism}" organism ' - f"for [{self.since} - {self.until}] range." - ) - else: - return accessions - - log.debug(message) - accessions.update(self.fetch_data()) - - return accessions - - def fetch_data(self) -> Set[str]: - """Retrieves accessions from the GEO meta DB.""" - - def match_keyword(row): - """ - Returns True if `row` matches `self.keyword` based regex. - Otherwise returns False. - """ - return re_keyword.match(" ".join((str(c) for c in row if c))) - - accessions = set() - - if not os.path.exists(self.db_path): - log.error("GEO meta database doesn't exist.") - return accessions - - connection = sqlite3.connect(self.db_path) - connection.row_factory = sqlite3.Row - connection.text_factory = lambda b: b.decode(errors="ignore") - entries = connection.execute(self.build_query()).fetchall() - connection.close() - - if self.keyword: - re_keyword = re.compile(f".*{self.keyword}.*", re.IGNORECASE) # Keyword regex. - entries = filter(match_keyword, entries) - - entries = ({key.lower(): entry[key] for key in entry.keys()} for entry in entries) - entries = set((AccessionBacklogEntry.create_from_ma_geo_entry(entry) for entry in entries)) - - if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) - accessions.update(entries) - - return accessions - - def get_ids(self) -> List[str]: - """Returns a combined list of passed GEO platform IDs.""" - ids = set() - - if self.options["gpl_id"]: - ids.update(self.options["gpl_id"]) - - if self.options["gpl_ids_file"]: - with open(self.options["gpl_ids_file"]) as gpl_ids_file: - ids.update((gpl_id.strip() for gpl_id in gpl_ids_file.readlines())) - - return sorted(ids) - - -class RNASeqAccessionAgent(AccessionAgentBase): - """ - RNA-Seq accession gathering agent. The data is fetched from - The European Nucleotide Archive (ENA) Portal. - See https://www.ebi.ac.uk/ena/portal/api/ for more information about the API - endpoints. - """ - - def __init__(self, options) -> None: - super().__init__(options) - - self.data_chunk_size = 10000 - self.data_url = "https://www.ebi.ac.uk/ena/portal/api/search" - self.ids = self.get_ids() - - def build_query(self, taxon_id: str = None) -> str: - """ - Returns a query to use for getting specific taxon ID accessions. - Some special characters must remain unquoted. - """ - - AND = " AND " - OR = " OR " - instrument_models = ( - "HiSeq X Five", - "HiSeq X Ten", - "Illumina Genome Analyzer II", - "Illumina Genome Analyzer IIx", - "Illumina Genome Analyzer", - "Illumina HiScanSQ", - "Illumina HiSeq 1000", - "Illumina HiSeq 1500", - "Illumina HiSeq 2000", - "Illumina HiSeq 2500", - "Illumina HiSeq 3000", - "Illumina HiSeq 4000", - "Illumina MiSeq", - "Illumina NovaSeq 6000", - "Ion Torrent Proton", - "Ion Torrent S5 XL", - "Ion Torrent S5", - "NextSeq 500", - "NextSeq 550", - ) - - instrument_models = OR.join((f'instrument_model="{im}"' for im in instrument_models)) - conditions = [ - # Relevant date fields: collection_date, collection_date_submitted, - # first_public, last_updated. - f"first_public >= {self.since}", - f"first_public <= {self.until}", - f"({instrument_models})", - 'library_source="TRANSCRIPTOMIC"', - 'library_strategy="RNA-Seq"', - ] - - if taxon_id: - conditions.append(f"tax_eq({taxon_id})") - elif self.keyword: - search_fields = ( - "assembly_software", - "bio_material", - "center_name", - "collected_by", - "experiment_title", - "host_body_site", - "instrument_model", - "instrument_platform", - "library_name", - "project_name", - "sample_title", - "sequencing_method", - "study_title", - ) - search_fields = OR.join( - (f'{sf}="*{self.keyword}*"' for sf in search_fields) - ) # Keyword regex. - conditions.append(f"({search_fields})") - elif self.organism: - # `host`: Natural (as opposed to laboratory) host to the organism from which sample - # was obtained. - # `host_scientific_name`: Scientific name of the natural (as opposed to laboratory) - # host to the organism from which sample was obtained. - # `scientific_name` Scientific name of the organism from which the sample was derived. - # Neither `host_scientific_name` nor `scientific_name` available for search. - # https://www.ebi.ac.uk/ena/portal/api/searchFields?dataPortal=ena&format=json&result=read_study - conditions.append(f'host="{self.organism}"') - - return quote(AND.join(conditions), safe='*()-="<>/ ') # Must remain unquoted. - - def collect_data(self) -> Set[str]: - """Gets new accessions from EBI ENA API.""" - accessions = set() - - if self.ids: - log.debug( - f"Getting RNA-Seq entries by taxon ID(s): " - f"{', '.join((str(idx) for idx in self.ids))} for [{self.since} - {self.until}] range." - ) - total = len(self.ids) - for idx, taxon_id in enumerate(self.ids): - if self.count and len(accessions) >= self.count: - break - - if total > 1: - log.debug(f"Getting entries for taxon ID {taxon_id}, {idx + 1} of {total}.") - accessions.update(self.fetch_data(taxon_id=taxon_id)) - elif self.keyword: - log.debug( - f'Getting RNA-Seq entries by "{self.keyword}" keyword ' - f"for [{self.since} - {self.until}] range." - ) - accessions.update(self.fetch_data()) - elif self.organism: - log.debug( - f'Getting entries by "{self.organism}" organism ' - f"for [{self.since} - {self.until}] range." - ) - accessions.update(self.fetch_data()) - - return accessions - - def fetch_data(self, taxon_id=None) -> Set[str]: - """ - Retrieves accessions from API search endpoint. - The API allows to set limit to 0 (get all in one request) but we do - it in a paginated fashion with `self.data_chunk_size` as a page size. - """ - - @retry(**self.retry_params) - def get_response(url, **kwargs): - """Gets response from an API endpoint.""" - return requests.post(url, **kwargs) - - accessions = set() - - fields = [ - "first_public", - "scientific_name", - "secondary_study_accession", - ] # For DRP/ERP/SRP-prefixed accessions. - data = { - "dataPortal": "ena", - # TODO(ark): add excludeAccessions/excludeAccessionType support. - "fields": ",".join(fields), # Use "all" to get all fields. - "format": "json", - "limit": self.data_chunk_size, - "offset": 0, - "query": self.build_query(taxon_id=taxon_id), - "result": "read_study", - "sortFields": fields, - } - - is_done = False - while not is_done: - log.debug( - f"Processing entries {data['offset'] + 1} - {data['offset'] + self.data_chunk_size}" - ) - entries = () - try: - response = get_response(self.data_url, data=data) - entries = response.json() - # TODO(ark): add `organism` when -o, --organism flag is used. - entries = ( - AccessionBacklogEntry.create_from_rnaseq_entry(entry) for entry in entries - ) - except JSONDecodeError: - is_done = True - except TypeError: - log.error(f"Couldn't get data from {self.data_url}. Response: {entries}") - data["offset"] += self.data_chunk_size - - if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) - accessions.update(entries) - - # Quit after getting a sufficient amount of accessions. - if self.count and len(accessions) >= self.count: - is_done = True - - return accessions - - def get_ids(self) -> List[str]: - """Returns a combined list of passed taxon IDs.""" - ids = set() - - if self.options["taxon_id"]: - ids.update(self.options["taxon_id"]) - - if self.options["taxon_ids_file"]: - with open(self.options["taxon_ids_file"]) as taxon_id_file: - ids.update((taxon_id.strip() for taxon_id in taxon_id_file.readlines())) - - return sorted(ids) + GatheredAccession.objects.bulk_create(entries) From 3fe5d8088d0862a1445cf002c4a4b1f067f4cb55 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 13 Sep 2022 17:17:19 -0700 Subject: [PATCH 041/220] Add a TODO. --- foreman/data_refinery_foreman/gatherer/agents/rna_seq.py | 1 + 1 file changed, 1 insertion(+) diff --git a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py index f9497f3ba..f54ba570a 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py +++ b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py @@ -34,6 +34,7 @@ def build_query(self, taxon_id: str = None) -> str: AND = " AND " OR = " OR " + # TODO(ark): extract instrument models to a config file. instrument_models = ( "HiSeq X Five", "HiSeq X Ten", From ee66ac812337f7cdf9101c7a906497d9e3ea75ba Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 13 Sep 2022 18:37:09 -0700 Subject: [PATCH 042/220] Fix empty response issue. --- foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py index b5314302b..541bd86d2 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py @@ -92,7 +92,7 @@ def get_response(url, **kwargs): logger.debug(f"Processing entries {range_start} - {range_end}") response = get_response(self.DATA_URL, params=params) - entries = response.json().get("hits") + entries = response.json().get("hits", ()) if entries: entries = ( GatheredAccession.create_from_ma_ae_entry(entry, organism=self.organism) From 5521286c64b4dc41a9ce070907157fce435c2cfa Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 14 Sep 2022 18:52:57 -0700 Subject: [PATCH 043/220] Address review comments. --- .../migrations/0071_gatheredaccession.py | 2 +- .../models/gathered_accession.py | 72 +++++++------------ .../gatherer/agents/base.py | 5 +- .../gatherer/agents/microarray_ae.py | 21 ++++-- .../gatherer/agents/microarray_geo.py | 23 ++++-- .../gatherer/agents/rna_seq.py | 24 +++++-- .../management/commands/gather_accessions.py | 60 ++++++++-------- 7 files changed, 112 insertions(+), 95 deletions(-) diff --git a/common/data_refinery_common/migrations/0071_gatheredaccession.py b/common/data_refinery_common/migrations/0071_gatheredaccession.py index a1740d96e..65d192b59 100644 --- a/common/data_refinery_common/migrations/0071_gatheredaccession.py +++ b/common/data_refinery_common/migrations/0071_gatheredaccession.py @@ -22,7 +22,7 @@ class Migration(migrations.Migration): verbose_name="ID", ), ), - ("code", models.TextField(unique=True)), + ("accession_code", models.TextField(unique=True)), ("created_at", models.DateTimeField(auto_now_add=True)), ("last_modified_at", models.DateTimeField(auto_now=True)), ("organism", models.TextField()), diff --git a/common/data_refinery_common/models/gathered_accession.py b/common/data_refinery_common/models/gathered_accession.py index 04b084533..e56ed615c 100644 --- a/common/data_refinery_common/models/gathered_accession.py +++ b/common/data_refinery_common/models/gathered_accession.py @@ -10,7 +10,7 @@ class GatheredAccession(models.Model): class Meta: db_table = "gathered_accessions" - code = models.TextField(unique=True) + accession_code = models.TextField(unique=True) created_at = models.DateTimeField(auto_now_add=True) last_modified_at = models.DateTimeField(auto_now=True) organism = models.TextField() @@ -21,64 +21,44 @@ class Meta: def __eq__(self, other: object) -> bool: """Returns True if two objects are equal. Otherwise returns False.""" - return isinstance(other, GatheredAccession) and self.code == other.code + return isinstance(other, GatheredAccession) and self.accession_code == other.accession_code def __hash__(self) -> int: """Returns accession object unique hash value.""" - return hash(self.code) + return hash(self.accession_code) def __str__(self) -> str: """Returns accession default string representation.""" - return ", ".join((self.code, self.technology, self.source, str(self.published_date.date()))) - - @staticmethod - def create_from_ma_ae_entry(entry, organism=None): - """Creates accession object from MicroArray ArrayExpress entry.""" - accession = GatheredAccession() - accession.code = entry["accession"] - accession.source = "ebi_biostudies" - accession.technology = "microarray" - - if organism: - accession.organism = organism - if "release_date" in entry: - accession.published_date = timezone.make_aware( - datetime.strptime(entry["release_date"], "%Y-%m-%d") + return ", ".join( + ( + self.accession_code, + self.technology, + self.source, + str(self.published_date.date()), ) - - return accession + ) @staticmethod - def create_from_ma_geo_entry(entry): - """Creates accession object from MicroArray GEO meta DB entry.""" + def create_from_external_entry(data, source, technology, organism=None): + """Creates accession object from MicroArray ArrayExpress entry.""" accession = GatheredAccession() - accession.code = entry["gse"] - accession.source = "geo_meta_db" - accession.technology = "microarray" - - if "organism" in entry: - accession.organism = entry["organism"].lower() - if "submission_date" in entry: - accession.published_date = timezone.make_aware( - datetime.strptime(entry["submission_date"], "%Y-%m-%d") - ) + accession.accession_code = ( + data.get("accession") or data.get("gse") or data.get("secondary_study_accession") + ) - return accession + organism = data.get("organism") or data.get("scientific_name") or organism + if organism: + accession.organism = organism.lower() - @staticmethod - def create_from_rnaseq_entry(entry): - """Creates accession object from RNA-Seq entry.""" - accession = GatheredAccession() - accession.code = entry["secondary_study_accession"] - accession.source = "ebi_ena_portal" - accession.technology = "rna-seq" + published_date = ( + data.get("first_public") or data.get("release_date") or data.get("submission_date") + ) + accession.published_date = timezone.make_aware( + datetime.strptime(published_date, "%Y-%m-%d") + ) - if "scientific_name" in entry: - accession.organism = entry["scientific_name"].lower() - if "first_public" in entry: - accession.published_date = timezone.make_aware( - datetime.strptime(entry["first_public"], "%Y-%m-%d") - ) + accession.source = source + accession.technology = technology return accession diff --git a/foreman/data_refinery_foreman/gatherer/agents/base.py b/foreman/data_refinery_foreman/gatherer/agents/base.py index 3754a4068..818bbf72c 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/base.py +++ b/foreman/data_refinery_foreman/gatherer/agents/base.py @@ -66,7 +66,10 @@ def populate_previous_accessions(self) -> None: # Gathered accessions. self.previous_accessions.update( - (entry["code"] for entry in GatheredAccession.objects.values("code")) + ( + entry["accession_code"] + for entry in GatheredAccession.objects.values("accession_code") + ) ) # Surveyed accessions. diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py index 541bd86d2..3bfcf08fe 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py @@ -1,5 +1,7 @@ -"""MicroArray ArrayExpress accession gathering automation. -Data source: https://www.ebi.ac.uk/biostudies/help""" +""" +MicroArray ArrayExpress accession gathering automation. +Data source: https://www.ebi.ac.uk/biostudies/help +""" from typing import List, Set @@ -13,7 +15,7 @@ logger = get_and_configure_logger(__name__) -class MicroArrayExpressAccessionAgent(AccessionAgentBase): +class AEAgent(AccessionAgentBase): """ MicroArray ArrayExpress accession gathering agent. The data is fetched from the BioStudies database. See https://www.ebi.ac.uk/biostudies/help and @@ -23,6 +25,9 @@ class MicroArrayExpressAccessionAgent(AccessionAgentBase): DATA_CHUNK_SIZE = 100 DATA_URL = "https://www.ebi.ac.uk/biostudies/api/v1/search" + SOURCE = "ebi-biostudies" + SOURCE_NAME = "microarray-ae" + TECHNOLOGY = "microarray" def build_query(self) -> dict: """Returns a query dict for getting array/organism specific accessions.""" @@ -95,7 +100,9 @@ def get_response(url, **kwargs): entries = response.json().get("hits", ()) if entries: entries = ( - GatheredAccession.create_from_ma_ae_entry(entry, organism=self.organism) + GatheredAccession.create_from_external_entry( + entry, self.SOURCE, self.TECHNOLOGY, organism=self.organism + ) for entry in entries ) params["page"] += 1 @@ -103,7 +110,11 @@ def get_response(url, **kwargs): is_done = True if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) + entries = ( + entry + for entry in entries + if entry.accession_code not in self.previous_accessions + ) accessions.update(entries) # Quit after getting a sufficient amount of accessions. diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py index 975c715b3..2500bcec5 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py +++ b/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py @@ -1,5 +1,8 @@ -"""MicroArray GEO accession gathering automation. -Data source: local SQLite meta DB from https://www.bioconductor.org/packages/release/bioc/html/GEOmetadb.html""" +""" +MicroArray GEO accession gathering automation. +Data source: local SQLite meta DB from +https://www.bioconductor.org/packages/release/bioc/html/GEOmetadb.html +""" import os import re @@ -13,7 +16,7 @@ logger = get_and_configure_logger(__name__) -class MicroArrayGEOAccessionAgent(AccessionAgentBase): +class GEOAgent(AccessionAgentBase): """ MicroArray GEO accession gathering agent. The data is fetched from a local SQLite GEO meta database. @@ -23,6 +26,9 @@ class MicroArrayGEOAccessionAgent(AccessionAgentBase): # Implement syncing procedure. # Update URL once the original file is available again. DB_PATH = "data/microarray/GEOmetadb.sqlite" + SOURCE = "geo-meta-db" + SOURCE_NAME = "microarray-geo" + TECHNOLOGY = "microarray" def build_query(self) -> str: """Returns a query for getting GEO accessions from the local SQLite meta DB.""" @@ -101,10 +107,17 @@ def match_keyword(row): entries = filter(match_keyword, entries) entries = ({key.lower(): entry[key] for key in entry.keys()} for entry in entries) - entries = set((GatheredAccession.create_from_ma_geo_entry(entry) for entry in entries)) + entries = set( + ( + GatheredAccession.create_from_external_entry(entry, self.SOURCE, self.TECHNOLOGY) + for entry in entries + ) + ) if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) + entries = ( + entry for entry in entries if entry.accession_code not in self.previous_accessions + ) accessions.update(entries) return accessions diff --git a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py index f54ba570a..577f815b8 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py +++ b/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py @@ -1,5 +1,7 @@ -"""RNA-Seq accession gathering automation. -Data source: https://www.ebi.ac.uk/ena/portal/api/""" +""" +RNA-Seq accession gathering automation. +Data source: https://www.ebi.ac.uk/ena/portal/api/ +""" from json.decoder import JSONDecodeError from typing import List, Set @@ -15,7 +17,7 @@ logger = get_and_configure_logger(__name__) -class RNASeqAccessionAgent(AccessionAgentBase): +class RNASeqAgent(AccessionAgentBase): """ RNA-Seq accession gathering agent. The data is fetched from The European Nucleotide Archive (ENA) Portal. @@ -25,6 +27,9 @@ class RNASeqAccessionAgent(AccessionAgentBase): DATA_CHUNK_SIZE = 10000 DATA_URL = "https://www.ebi.ac.uk/ena/portal/api/search" + SOURCE = "ebi-ena-portal" + SOURCE_NAME = "rna-seq" + TECHNOLOGY = "rna-seq" def build_query(self, taxon_id: str = None) -> str: """ @@ -174,7 +179,12 @@ def get_response(url, **kwargs): try: response = get_response(self.DATA_URL, data=data) entries = response.json() - entries = (GatheredAccession.create_from_rnaseq_entry(entry) for entry in entries) + entries = ( + GatheredAccession.create_from_external_entry( + entry, self.SOURCE, self.TECHNOLOGY + ) + for entry in entries + ) except JSONDecodeError: is_done = True except TypeError: @@ -182,7 +192,11 @@ def get_response(url, **kwargs): data["offset"] += self.DATA_CHUNK_SIZE if self.previous_accessions: - entries = (entry for entry in entries if entry.code not in self.previous_accessions) + entries = ( + entry + for entry in entries + if entry.accession_code not in self.previous_accessions + ) accessions.update(entries) # Quit after getting a sufficient amount of accessions. diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 445245d3a..2b073ef45 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -11,12 +11,13 @@ import re from django.core.management.base import BaseCommand +from django.db.utils import IntegrityError from data_refinery_common.logging import get_and_configure_logger from data_refinery_common.models.gathered_accession import GatheredAccession -from data_refinery_foreman.gatherer.agents.microarray_ae import MicroArrayExpressAccessionAgent -from data_refinery_foreman.gatherer.agents.microarray_geo import MicroArrayGEOAccessionAgent -from data_refinery_foreman.gatherer.agents.rna_seq import RNASeqAccessionAgent +from data_refinery_foreman.gatherer.agents.microarray_ae import AEAgent +from data_refinery_foreman.gatherer.agents.microarray_geo import GEOAgent +from data_refinery_foreman.gatherer.agents.rna_seq import RNASeqAgent logger = get_and_configure_logger(__name__) @@ -24,11 +25,8 @@ class Command(BaseCommand): """Creates agents and runs actual accession gathering.""" - DATA_SOURCE_MA_AE = "microarray-ae" - DATA_SOURCE_MA_GEO = "microarray-geo" - DATA_SOURCE_RNA_SEQ = "rna-seq" - DATA_SOURCES = (DATA_SOURCE_MA_AE, DATA_SOURCE_MA_GEO, DATA_SOURCE_RNA_SEQ) - + DATA_AGENTS = (AEAgent, GEOAgent, RNASeqAgent) + DATA_SOURCE_NAMES = [agent.SOURCE_NAME for agent in DATA_AGENTS] RE_ACCESSION = re.compile(r"(\D+)(\d+)") RE_DATE = re.compile(r"\d{4}-\d{2}-\d{2}") @@ -164,43 +162,43 @@ def validate_args(self, options) -> None: keyword = options["keyword"] organism = options["organism"] - sources = options["source"] or self.DATA_SOURCES + source_names = options["source"] or self.DATA_SOURCE_NAMES - for source in sources: - if source in self.DATA_SOURCES: + for source_name in source_names: + if source_name in self.DATA_SOURCE_NAMES: continue errors.append( - f"Unknown source: {source}. Supported sources: {', '.join(self.DATA_SOURCES)}" + f"Unknown source: {source_name}. Supported sources: {', '.join(self.DATA_SOURCE_NAMES)}" ) - if self.DATA_SOURCE_MA_AE in sources: + if AEAgent.SOURCE_NAME in source_names: ids = options["ae_id"] or options["ae_ids_file"] if not (ids or keyword or organism): errors.append( ( "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " "ArrayExpress ID(s) [--ae-id, --ae-ids-file] must be specified for " - f"'{self.DATA_SOURCE_MA_AE}' source." + f"'{AEAgent.SOURCE_NAME}' source." ) ) - if self.DATA_SOURCE_MA_GEO in sources: + if GEOAgent.SOURCE_NAME in source_names: ids = options["gpl_id"] or options["gpl_ids_file"] if not (ids or keyword or organism): errors.append( ( "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] or " "GEO platform ID(s) [--gpl-id, --gpl-ids-file] must be specified for " - f"'{self.DATA_SOURCE_MA_GEO}' source." + f"'{GEOAgent.SOURCE_NAME}' source." ) ) - if self.DATA_SOURCE_RNA_SEQ in sources: + if RNASeqAgent.SOURCE_NAME in source_names: ids = options["taxon_id"] or options["taxon_ids_file"] if not (ids or keyword or organism): errors.append( ( "Exactly one of the keyword [-k, --keyword], organism [-o, --organism] " "or taxon ID(s) [--taxon-id, --taxon-ids-file] must be specified for " - f"'{self.DATA_SOURCE_RNA_SEQ}' source." + f"'{RNASeqAgent.SOURCE_NAME}' source." ) ) @@ -213,26 +211,21 @@ def handle(self, *args, **options): self.set_verbosity_level(options) agents = list() - sources = options["source"] or self.DATA_SOURCES - - if self.DATA_SOURCE_RNA_SEQ in sources: - agents.append(RNASeqAccessionAgent(options)) - - if self.DATA_SOURCE_MA_AE in sources: - agents.append(MicroArrayExpressAccessionAgent(options)) - - if self.DATA_SOURCE_MA_GEO in sources: - agents.append(MicroArrayGEOAccessionAgent(options)) + sources_names = options["source"] or self.DATA_SOURCE_NAMES + for cls in self.DATA_AGENTS: + if cls.SOURCE_NAME not in sources_names: + continue + agents.append(cls(options)) entries = set() for agent in agents: entries.update(agent.collect_data()) entries = sorted( # Sort the resulting list. - (entry for entry in entries if self.RE_ACCESSION.match(entry.code)), + (entry for entry in entries if self.RE_ACCESSION.match(entry.accession_code)), key=lambda entry: ( - self.RE_ACCESSION.match(entry.code).group(1), - int(self.RE_ACCESSION.match(entry.code).group(2)), + self.RE_ACCESSION.match(entry.accession_code).group(1), + int(self.RE_ACCESSION.match(entry.accession_code).group(2)), ), ) # Limit the number of output entries. @@ -245,4 +238,7 @@ def handle(self, *args, **options): output = "No accessions found." print(output) else: - GatheredAccession.objects.bulk_create(entries) + try: + GatheredAccession.objects.bulk_create(entries) + except IntegrityError as e: + logger.exception(f"Could not save new accessions to the database: {e}") From 6d3e17988765f045a69ebac8618a6fffad55a907 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 21 Sep 2022 11:05:24 -0700 Subject: [PATCH 044/220] Rename agent files. --- .../gatherer/agents/{microarray_ae.py => ae_agent.py} | 0 .../gatherer/agents/{microarray_geo.py => geo_agent.py} | 0 .../gatherer/agents/{rna_seq.py => rnaseq_agent.py} | 0 .../gatherer/management/commands/gather_accessions.py | 6 +++--- 4 files changed, 3 insertions(+), 3 deletions(-) rename foreman/data_refinery_foreman/gatherer/agents/{microarray_ae.py => ae_agent.py} (100%) rename foreman/data_refinery_foreman/gatherer/agents/{microarray_geo.py => geo_agent.py} (100%) rename foreman/data_refinery_foreman/gatherer/agents/{rna_seq.py => rnaseq_agent.py} (100%) diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py b/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py similarity index 100% rename from foreman/data_refinery_foreman/gatherer/agents/microarray_ae.py rename to foreman/data_refinery_foreman/gatherer/agents/ae_agent.py diff --git a/foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py b/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py similarity index 100% rename from foreman/data_refinery_foreman/gatherer/agents/microarray_geo.py rename to foreman/data_refinery_foreman/gatherer/agents/geo_agent.py diff --git a/foreman/data_refinery_foreman/gatherer/agents/rna_seq.py b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py similarity index 100% rename from foreman/data_refinery_foreman/gatherer/agents/rna_seq.py rename to foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 2b073ef45..554b74350 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -15,9 +15,9 @@ from data_refinery_common.logging import get_and_configure_logger from data_refinery_common.models.gathered_accession import GatheredAccession -from data_refinery_foreman.gatherer.agents.microarray_ae import AEAgent -from data_refinery_foreman.gatherer.agents.microarray_geo import GEOAgent -from data_refinery_foreman.gatherer.agents.rna_seq import RNASeqAgent +from data_refinery_foreman.gatherer.agents.ae_agent import AEAgent +from data_refinery_foreman.gatherer.agents.geo_agent import GEOAgent +from data_refinery_foreman.gatherer.agents.rnaseq_agent import RNASeqAgent logger = get_and_configure_logger(__name__) From 36c9f8a2067c1a6da7a152d816d5a6b4538ab9dc Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 2 Nov 2022 10:36:07 -0700 Subject: [PATCH 045/220] Update API Docker images. Reorder and clean up the instructions. --- api/dockerfiles/Dockerfile.api_local | 16 +++++++-------- api/dockerfiles/Dockerfile.api_production | 24 +++++++++++------------ 2 files changed, 18 insertions(+), 22 deletions(-) diff --git a/api/dockerfiles/Dockerfile.api_local b/api/dockerfiles/Dockerfile.api_local index f52c29d4f..05dd00df1 100644 --- a/api/dockerfiles/Dockerfile.api_local +++ b/api/dockerfiles/Dockerfile.api_local @@ -1,27 +1,25 @@ FROM python:3.8.5-slim-buster -# Install libpq-dev for psycopg2 +# Install gcc and libpq-dev for psycopg2. RUN apt-get update -qq -RUN apt-get install -y libpq-dev gcc +RUN apt-get install -y \ + gcc \ + libpq-dev RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user RUN pip install --upgrade pip -COPY config/ config/ - COPY api/requirements.txt . - RUN pip install -r requirements.txt -COPY common/dist/data-refinery-common-* common/ - # Get the latest version from the dist directory. -RUN pip install \ - common/$(ls common -1 | sort --version-sort | tail -1) +COPY common/dist/data-refinery-common-* common/ +RUN pip install common/$(ls common -1 | sort --version-sort | tail -1) COPY api/ . +COPY config/ config/ ARG SYSTEM_VERSION diff --git a/api/dockerfiles/Dockerfile.api_production b/api/dockerfiles/Dockerfile.api_production index 2de11c2b7..a432610ff 100644 --- a/api/dockerfiles/Dockerfile.api_production +++ b/api/dockerfiles/Dockerfile.api_production @@ -1,30 +1,28 @@ FROM python:3.8.5-buster -# Install libpq-dev and gcc for psycopg2 +# Install gcc and libpq-dev for psycopg2. RUN apt-get update -qq -RUN apt-get install -y libpq-dev gcc +RUN apt-get install -y \ + gcc \ + libpq-dev RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -COPY config/ config/ - COPY api/requirements.txt . - RUN pip install -r requirements.txt +# Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ +RUN pip install common/$(ls common -1 | sort --version-sort | tail -1) -# Get the latest version from the dist directory. -RUN pip install \ - common/$(ls common -1 | sort --version-sort | tail -1) +# uWSGI. +RUN pip install uwsgi COPY api/ . +COPY config/ config/ -# uWSGI -RUN pip install uwsgi RUN chmod +x /home/user/collect_and_run_uwsgi.sh - RUN mkdir -p /tmp/www/static RUN chown user /tmp/www/static @@ -34,8 +32,8 @@ ENV SYSTEM_VERSION $SYSTEM_VERSION USER user -# We collect Django's static files and expose them -# as a volume so that Nginx can serve them directly. +# We collect Django's static files and expose them as a volume so that Nginx +# can serve them directly. VOLUME /tmp/www/static EXPOSE 8081 From 50ce1e0e9eb0393abd1ed40ebc342858d0baf156 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 2 Nov 2022 10:44:26 -0700 Subject: [PATCH 046/220] Update foreman Docker image. Reorder and clean up the instructions. --- foreman/dockerfiles/Dockerfile.foreman | 28 ++++++++++++-------------- 1 file changed, 13 insertions(+), 15 deletions(-) diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 8c09c6888..8b7b9f239 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -1,40 +1,38 @@ FROM ubuntu:18.04 +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG C.UTF-8 + RUN apt-get update -qq RUN apt-get install -y software-properties-common RUN add-apt-repository ppa:apt-fast/stable + RUN apt-get update -qq RUN apt-get -y install apt-fast - -RUN apt-fast update -qq && \ - apt-fast install -y \ +RUN apt-fast update -qq +RUN apt-fast install -y \ + gcc \ + libpq-dev \ python3 \ python3-pip RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - RUN pip3 install --upgrade pip -COPY config config -COPY .boto .boto - COPY foreman/requirements.txt . - # The base image does not have Python 2.X on it at all, so all calls # to pip or python are by default calls to pip3 or python3 RUN pip3 install -r requirements.txt -COPY common/dist/data-refinery-common-* common/ - # Get the latest version from the dist directory. -RUN pip3 install \ - common/$(ls common -1 | sort --version-sort | tail -1) +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +COPY .boto .boto +COPY config config COPY foreman/ . ARG SYSTEM_VERSION From bba6fbee71740aa13e49cbe85e71f016215c5f85 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 2 Nov 2022 15:32:16 -0700 Subject: [PATCH 047/220] Move all tests to tests directory. Add separate settings file (tests/settings.py). Refactor import according to the new structure. --- .pre-commit-config.yaml | 2 +- common/data_refinery_common/test_imports.py | 13 ------------ .../data_refinery_common/test_microarray.py | 16 -------------- common/tests/__init__.py | 0 .../data/microarray}/97_(Mouse430_2).CEL.gz | Bin .../data/microarray}/C30057.CEL.gz | Bin .../data/microarray}/CONTROL6.cel.gz | Bin .../data/microarray}/SG2_u34a.CEL.gz | Bin common/tests/models/__init__.py | 0 .../models/test_jobs.py | 0 .../models/test_models.py | 0 .../models/test_ontology_term.py | 6 ++++-- .../models/test_organisms.py | 3 +-- common/tests/settings.py | 5 +++++ common/tests/test_imports.py | 13 ++++++++++++ .../test_job_management.py | 0 common/tests/test_microarray.py | 20 ++++++++++++++++++ .../test_utils.py | 2 +- 18 files changed, 45 insertions(+), 35 deletions(-) delete mode 100644 common/data_refinery_common/test_imports.py delete mode 100644 common/data_refinery_common/test_microarray.py create mode 100644 common/tests/__init__.py rename common/{test-files => tests/data/microarray}/97_(Mouse430_2).CEL.gz (100%) rename common/{test-files => tests/data/microarray}/C30057.CEL.gz (100%) rename common/{test-files => tests/data/microarray}/CONTROL6.cel.gz (100%) rename common/{test-files => tests/data/microarray}/SG2_u34a.CEL.gz (100%) create mode 100644 common/tests/models/__init__.py rename common/{data_refinery_common => tests}/models/test_jobs.py (100%) rename common/{data_refinery_common => tests}/models/test_models.py (100%) rename common/{data_refinery_common => tests}/models/test_ontology_term.py (95%) rename common/{data_refinery_common => tests}/models/test_organisms.py (99%) create mode 100644 common/tests/settings.py create mode 100644 common/tests/test_imports.py rename common/{data_refinery_common => tests}/test_job_management.py (100%) create mode 100644 common/tests/test_microarray.py rename common/{data_refinery_common => tests}/test_utils.py (98%) diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index d024704da..b651ce24a 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -22,7 +22,7 @@ repos: - id: isort - repo: https://github.com/psf/black - rev: 19.10b0 + rev: 22.3.0 hooks: - id: black args: [--line-length=100] diff --git a/common/data_refinery_common/test_imports.py b/common/data_refinery_common/test_imports.py deleted file mode 100644 index a637ad54a..000000000 --- a/common/data_refinery_common/test_imports.py +++ /dev/null @@ -1,13 +0,0 @@ -from django.test import TestCase, tag - - -class ImportTestCase(TestCase): - def test_imports(self): - # Make sure we can import the common tests - import data_refinery_common.models.test_jobs - import data_refinery_common.models.test_models - import data_refinery_common.models.test_ontology_term - import data_refinery_common.models.test_organisms - import data_refinery_common.test_job_management - import data_refinery_common.test_microarray - import data_refinery_common.test_utils diff --git a/common/data_refinery_common/test_microarray.py b/common/data_refinery_common/test_microarray.py deleted file mode 100644 index a2f6d9f91..000000000 --- a/common/data_refinery_common/test_microarray.py +++ /dev/null @@ -1,16 +0,0 @@ -from django.test import TestCase - -from data_refinery_common import microarray - -CEL_FILE_HUMAN = "test-files/C30057.CEL.gz" -CEL_FILE_RAT = "test-files/SG2_u34a.CEL.gz" -CEL_FILE_MOUSE = "test-files/97_(Mouse430_2).CEL.gz" -CEL_FILE_ZEBRAFISH = "test-files/CONTROL6.cel.gz" - - -class MicroarrayTestCase(TestCase): - def test_get_platform_from_CEL(self): - self.assertEqual("hgu95av2", microarray.get_platform_from_CEL(CEL_FILE_HUMAN)) - self.assertEqual("rgu34a", microarray.get_platform_from_CEL(CEL_FILE_RAT)) - self.assertEqual("mouse4302", microarray.get_platform_from_CEL(CEL_FILE_MOUSE)) - self.assertEqual("zebgene11st", microarray.get_platform_from_CEL(CEL_FILE_ZEBRAFISH)) diff --git a/common/tests/__init__.py b/common/tests/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/common/test-files/97_(Mouse430_2).CEL.gz b/common/tests/data/microarray/97_(Mouse430_2).CEL.gz similarity index 100% rename from common/test-files/97_(Mouse430_2).CEL.gz rename to common/tests/data/microarray/97_(Mouse430_2).CEL.gz diff --git a/common/test-files/C30057.CEL.gz b/common/tests/data/microarray/C30057.CEL.gz similarity index 100% rename from common/test-files/C30057.CEL.gz rename to common/tests/data/microarray/C30057.CEL.gz diff --git a/common/test-files/CONTROL6.cel.gz b/common/tests/data/microarray/CONTROL6.cel.gz similarity index 100% rename from common/test-files/CONTROL6.cel.gz rename to common/tests/data/microarray/CONTROL6.cel.gz diff --git a/common/test-files/SG2_u34a.CEL.gz b/common/tests/data/microarray/SG2_u34a.CEL.gz similarity index 100% rename from common/test-files/SG2_u34a.CEL.gz rename to common/tests/data/microarray/SG2_u34a.CEL.gz diff --git a/common/tests/models/__init__.py b/common/tests/models/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/common/data_refinery_common/models/test_jobs.py b/common/tests/models/test_jobs.py similarity index 100% rename from common/data_refinery_common/models/test_jobs.py rename to common/tests/models/test_jobs.py diff --git a/common/data_refinery_common/models/test_models.py b/common/tests/models/test_models.py similarity index 100% rename from common/data_refinery_common/models/test_models.py rename to common/tests/models/test_models.py diff --git a/common/data_refinery_common/models/test_ontology_term.py b/common/tests/models/test_ontology_term.py similarity index 95% rename from common/data_refinery_common/models/test_ontology_term.py rename to common/tests/models/test_ontology_term.py index 65340349d..4b2200059 100644 --- a/common/data_refinery_common/models/test_ontology_term.py +++ b/common/tests/models/test_ontology_term.py @@ -108,12 +108,14 @@ def test_import_cellosaurus(self, mock_api_call): created_terms = OntologyTerm.import_entire_ontology("cvcl") self.assertEqual( - OntologyTerm.objects.all().count(), 34, # This is the number I counted in the file + OntologyTerm.objects.all().count(), + 34, # This is the number I counted in the file ) self.assertEqual(OntologyTerm.objects.all().count(), created_terms) self.assertEqual( - "#W7079", OntologyTerm.get_or_create_from_api("CVCL:E549").human_readable_name, + "#W7079", + OntologyTerm.get_or_create_from_api("CVCL:E549").human_readable_name, ) mock_api_call.assert_not_called() diff --git a/common/data_refinery_common/models/test_organisms.py b/common/tests/models/test_organisms.py similarity index 99% rename from common/data_refinery_common/models/test_organisms.py rename to common/tests/models/test_organisms.py index b84dbaa65..4fc14540b 100644 --- a/common/data_refinery_common/models/test_organisms.py +++ b/common/tests/models/test_organisms.py @@ -231,8 +231,7 @@ def test_invalid_ids_cause_exceptions(self, mock_get): @patch("data_refinery_common.models.organism.requests.get") def test_unfound_names_raise(self, mock_get): - """If we can't find the taxonomy id, it's likely a bad organism name. - """ + """If we can't find the taxonomy id, it's likely a bad organism name.""" mock_get.return_value = Mock(ok=True) mock_get.return_value.text = ESEARCH_NOT_FOUND_XML diff --git a/common/tests/settings.py b/common/tests/settings.py new file mode 100644 index 000000000..b821f1f9a --- /dev/null +++ b/common/tests/settings.py @@ -0,0 +1,5 @@ +""" +Django settings for data_refinery_common project tests. +""" + +from data_refinery_common.settings import * diff --git a/common/tests/test_imports.py b/common/tests/test_imports.py new file mode 100644 index 000000000..b3af0ac3e --- /dev/null +++ b/common/tests/test_imports.py @@ -0,0 +1,13 @@ +from django.test import TestCase + + +class ImportTestCase(TestCase): + def test_imports(self): + # Make sure we can import the common tests + import tests.models.test_jobs + import tests.models.test_models + import tests.models.test_ontology_term + import tests.models.test_organisms + import tests.test_job_management + import tests.test_microarray + import tests.test_utils diff --git a/common/data_refinery_common/test_job_management.py b/common/tests/test_job_management.py similarity index 100% rename from common/data_refinery_common/test_job_management.py rename to common/tests/test_job_management.py diff --git a/common/tests/test_microarray.py b/common/tests/test_microarray.py new file mode 100644 index 000000000..4bc66d039 --- /dev/null +++ b/common/tests/test_microarray.py @@ -0,0 +1,20 @@ +from django.test import TestCase + +from data_refinery_common import microarray + + +class MicroarrayTestCase(TestCase): + def test_get_platform_from_CEL(self): + self.assertEqual( + "hgu95av2", microarray.get_platform_from_CEL("test/data/microarray/C30057.CEL.gz") + ) + self.assertEqual( + "rgu34a", microarray.get_platform_from_CEL("test/data/microarray/SG2_u34a.CEL.gz") + ) + self.assertEqual( + "mouse4302", + microarray.get_platform_from_CEL("test/data/microarray/97_(Mouse430_2).CEL.gz"), + ) + self.assertEqual( + "zebgene11st", microarray.get_platform_from_CEL("test/data/microarray/CONTROL6.cel.gz") + ) diff --git a/common/data_refinery_common/test_utils.py b/common/tests/test_utils.py similarity index 98% rename from common/data_refinery_common/test_utils.py rename to common/tests/test_utils.py index 71c8772ee..bcf9ee01a 100644 --- a/common/data_refinery_common/test_utils.py +++ b/common/tests/test_utils.py @@ -89,7 +89,7 @@ def test_readable_affymetrix_names(self): self.assertTrue(readable_platform_names["xenopuslaevis"] == expected_readable_name) def test_get_normalized_platform(self): - """ Test a particular normaization we need to perform """ + """Test a particular normaization we need to perform""" self.assertEqual(utils.get_normalized_platform("hugene10stv1"), "hugene10st") self.assertEqual(utils.get_normalized_platform("hugene10stv2"), "hugene10st") From 2f6ff4a36d263433241b75629bef7ad041227652 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 17 Nov 2022 14:46:30 -0800 Subject: [PATCH 048/220] Workers Docker images refactoring. --- common/{ => R}/install_devtools.R | 12 +- .../dependencies}/README.md | 0 .../affymetrix/bioc/dependencies.R | 221 ++++++++++++++++++ .../affymetrix/cran}/dependencies.R | 0 .../affymetrix/cran}/packages.txt | 0 .../affymetrix/cran}/versions.tsv | 0 .../affymetrix/install_affy_only.R | 38 +++ .../affymetrix}/install_ensg_pkgs.R | 8 +- .../illumina/bioc/dependencies.R} | 0 .../illumina/cran}/dependencies.R | 0 .../dependencies/illumina/cran}/packages.txt | 0 .../dependencies/illumina/cran}/versions.tsv | 0 workers/{ => R/dependencies}/install_bioc.R | 10 +- .../dependencies}/install_downloader_R_only.R | 14 +- .../dependencies/no_op/cran}/dependencies.R | 0 .../dependencies/no_op/cran}/packages.txt | 0 .../dependencies/no_op/cran}/versions.tsv | 0 .../dependencies/no_op/install_gene_convert.R | 43 ++++ .../dependencies/qn/bioc/dependencies.R} | 12 +- .../dependencies/qn/cran}/dependencies.R | 0 .../dependencies/qn/cran}/packages.txt | 0 .../dependencies/qn/cran}/versions.tsv | 0 .../dependencies/tximport/cran/dependencies.R | 25 ++ .../dependencies/tximport/cran}/packages.txt | 0 .../dependencies/tximport/cran}/versions.tsv | 0 .../dependencies/tximport/install_tximport.R | 7 + .../R_dependencies/tximport/dependencies.R | 25 -- workers/affymetrix_dependencies.R | 220 ----------------- workers/ccache.conf | 5 + workers/dockerfiles/Dockerfile.affymetrix | 90 ++----- .../dockerfiles/Dockerfile.affymetrix_local | 12 +- workers/dockerfiles/Dockerfile.compendia | 154 ++++++------ workers/dockerfiles/Dockerfile.downloaders | 87 ++----- workers/dockerfiles/Dockerfile.illumina | 80 +------ workers/dockerfiles/Dockerfile.no_op | 90 ++----- workers/dockerfiles/Dockerfile.salmon | 114 +++------ workers/dockerfiles/Dockerfile.smasher | 90 ++----- workers/dockerfiles/Dockerfile.transcriptome | 90 ++----- workers/dockerfiles/Dockerfile.worker_base | 68 ++++++ workers/install_affy_only.R | 38 --- workers/install_gene_convert.R | 43 ---- workers/install_tximport.R | 7 - 42 files changed, 650 insertions(+), 953 deletions(-) rename common/{ => R}/install_devtools.R (95%) rename workers/{R_dependencies => R/dependencies}/README.md (100%) create mode 100644 workers/R/dependencies/affymetrix/bioc/dependencies.R rename workers/{R_dependencies/affymetrix => R/dependencies/affymetrix/cran}/dependencies.R (100%) rename workers/{R_dependencies/affymetrix => R/dependencies/affymetrix/cran}/packages.txt (100%) rename workers/{R_dependencies/affymetrix => R/dependencies/affymetrix/cran}/versions.tsv (100%) create mode 100644 workers/R/dependencies/affymetrix/install_affy_only.R rename workers/{ => R/dependencies/affymetrix}/install_ensg_pkgs.R (89%) rename workers/{illumina_dependencies.R => R/dependencies/illumina/bioc/dependencies.R} (100%) rename workers/{R_dependencies/illumina => R/dependencies/illumina/cran}/dependencies.R (100%) rename workers/{R_dependencies/illumina => R/dependencies/illumina/cran}/packages.txt (100%) rename workers/{R_dependencies/illumina => R/dependencies/illumina/cran}/versions.tsv (100%) rename workers/{ => R/dependencies}/install_bioc.R (58%) rename workers/{ => R/dependencies}/install_downloader_R_only.R (54%) rename workers/{R_dependencies/no_op => R/dependencies/no_op/cran}/dependencies.R (100%) rename workers/{R_dependencies/no_op => R/dependencies/no_op/cran}/packages.txt (100%) rename workers/{R_dependencies/no_op => R/dependencies/no_op/cran}/versions.tsv (100%) create mode 100644 workers/R/dependencies/no_op/install_gene_convert.R rename workers/{qn_dependencies.R => R/dependencies/qn/bioc/dependencies.R} (61%) rename workers/{R_dependencies/qn => R/dependencies/qn/cran}/dependencies.R (100%) rename workers/{R_dependencies/qn => R/dependencies/qn/cran}/packages.txt (100%) rename workers/{R_dependencies/qn => R/dependencies/qn/cran}/versions.tsv (100%) create mode 100644 workers/R/dependencies/tximport/cran/dependencies.R rename workers/{R_dependencies/tximport => R/dependencies/tximport/cran}/packages.txt (100%) rename workers/{R_dependencies/tximport => R/dependencies/tximport/cran}/versions.tsv (100%) create mode 100644 workers/R/dependencies/tximport/install_tximport.R delete mode 100644 workers/R_dependencies/tximport/dependencies.R delete mode 100644 workers/affymetrix_dependencies.R create mode 100644 workers/ccache.conf create mode 100644 workers/dockerfiles/Dockerfile.worker_base delete mode 100644 workers/install_affy_only.R delete mode 100644 workers/install_gene_convert.R delete mode 100644 workers/install_tximport.R diff --git a/common/install_devtools.R b/common/R/install_devtools.R similarity index 95% rename from common/install_devtools.R rename to common/R/install_devtools.R index 678418f19..e241152ea 100644 --- a/common/install_devtools.R +++ b/common/R/install_devtools.R @@ -16,9 +16,9 @@ # Cranlock was used to find the versions of dependencies to install # Treat warnings as errors, set CRAN mirror, and set parallelization: -options(warn=2) -options(repos=structure(c(CRAN="https://cloud.r-project.org/"))) -options(Ncpus=parallel::detectCores()) +options(warn = 2) +options(repos = structure(c(CRAN = "https://cloud.r-project.org/"))) +options(Ncpus = parallel::detectCores()) install_package_version <- function(package_name, version) { @@ -31,18 +31,18 @@ install_package_version <- function(package_name, version) { package_url <- paste0("https://cloud.r-project.org/src/contrib/", package_tarball) # Give CRAN a full minute to timeout since it's not always the most reliable. - curl_result <- system(paste0("curl --head --connect-timeout 60 ", package_url), intern=TRUE) + curl_result <- system(paste0("curl --head --connect-timeout 60 ", package_url), intern = TRUE) if (grepl("404", curl_result[1])) { package_url <- paste0("https://cloud.r-project.org/src/contrib/Archive/", package_name, "/", package_tarball) # Make sure the package actually exists in the archive! - curl_result <- system(paste0("curl --head --connect-timeout 120 ", package_url), intern=TRUE) + curl_result <- system(paste0("curl --head --connect-timeout 120 ", package_url), intern = TRUE) if (grepl("404", curl_result[1])) { stop(paste("Package", package_name, "version", version, "does not exist!")) } } - install.packages(package_url) + install.packages(package_url, Ncpus = 32) } # Generated using cranlock diff --git a/workers/R_dependencies/README.md b/workers/R/dependencies/README.md similarity index 100% rename from workers/R_dependencies/README.md rename to workers/R/dependencies/README.md diff --git a/workers/R/dependencies/affymetrix/bioc/dependencies.R b/workers/R/dependencies/affymetrix/bioc/dependencies.R new file mode 100644 index 000000000..1570292e0 --- /dev/null +++ b/workers/R/dependencies/affymetrix/bioc/dependencies.R @@ -0,0 +1,221 @@ +# Turn warnings into errors because biocLite throws warnings instead # of error if it fails to install something. +options(warn = 2) +options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) +options(Ncpus = parallel::detectCores()) + +# Bioconductor packages, installed by devtools::install_url() + +# Helper function that installs a list of packages using the input URLs +install_with_url <- function(urls) { + pkg_ids <- devtools::install_url(urls) + if (any(is.na(pkg_ids))) { + pkg_fails <- paste(urls[is.na(pkg_ids)], collapse = "; ") + stop(paste("Failed to install package(s):", pkg_fails)) + } + return(pkg_ids) +} + +devtools::install_version("dplyr", version = "1.0.2") +devtools::install_version("locfit", version = "1.5-9.4") + +bioc_pkg_urls <- c( + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/GEOquery_2.56.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/SCAN.UPC_2.30.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/affy_1.66.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/genefilter_1.70.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/sva_3.36.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz" +) +install_with_url(bioc_pkg_urls) + +# Invoke another R script to install BrainArray ensg packages +source("install_ensg_pkgs.R") + +# Install Bioconductor platform design (pd) packages +pd_experiment_pkgs <- c( + "https://bioconductor.org/packages/3.11/data/experiment/src/contrib/pd.atdschip.tiling_0.26.0.tar.gz" +) +install_with_url(pd_experiment_pkgs) + +pd_annotation_pkgs <- c( + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.081229.hg18.promoter.medip.hx1_0.99.4.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.2006.07.18.hg18.refseq.promoter_1.8.1.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.2006.07.18.mm8.refseq.promoter_0.99.3.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.2006.10.31.rn34.refseq.promoter_0.99.3.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.ag_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.aragene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.aragene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.ath1.121501_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.barley1_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.bovgene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.bovgene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.bovine_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.bsubtilis_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cangene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cangene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.canine_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.canine.2_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.celegans_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.charm.hg18.example_0.99.4.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.chicken_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.chigene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.chigene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.chogene.2.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.chogene.2.1.st_3.12.0.tar.gz", + 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"https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cyngene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cyngene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cyrgene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cyrgene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cytogenetics.array_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.drogene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.drogene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.drosgenome1_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.drosophila.2_3.12.0.tar.gz", + 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"https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rabgene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rabgene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rae230a_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rae230b_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.raex.1.0.st.v1_3.14.1.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.ragene.1.0.st.v1_3.14.1.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.ragene.1.1.st.v1_3.14.1.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.ragene.2.0.st_3.14.1.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.ragene.2.1.st_3.14.1.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rat230.2_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rcngene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rcngene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rg.u34a_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rg.u34b_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rg.u34c_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rhegene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rhegene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rhesus_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rice_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rjpgene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rjpgene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rn.u34_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rta.1.0_3.12.2.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rusgene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rusgene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.s.aureus_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.soybean_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.soygene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.soygene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.sugar.cane_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.tomato_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.u133.x3p_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.vitis.vinifera_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.wheat_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.x.laevis.2_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.x.tropicalis_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.xenopus.laevis_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.yeast.2_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.yg.s98_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.zebgene.1.0.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.zebgene.1.1.st_3.12.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.zebrafish_3.12.0.tar.gz" +) +install_with_url(pd_annotation_pkgs) + +# Load this libraries because apparently just installing it isn't +# enough to verify that the correct versions of dependencies are installed. +library("foreach") diff --git a/workers/R_dependencies/affymetrix/dependencies.R b/workers/R/dependencies/affymetrix/cran/dependencies.R similarity index 100% rename from workers/R_dependencies/affymetrix/dependencies.R rename to workers/R/dependencies/affymetrix/cran/dependencies.R diff --git a/workers/R_dependencies/affymetrix/packages.txt b/workers/R/dependencies/affymetrix/cran/packages.txt similarity index 100% rename from workers/R_dependencies/affymetrix/packages.txt rename to workers/R/dependencies/affymetrix/cran/packages.txt diff --git a/workers/R_dependencies/affymetrix/versions.tsv b/workers/R/dependencies/affymetrix/cran/versions.tsv similarity index 100% rename from workers/R_dependencies/affymetrix/versions.tsv rename to workers/R/dependencies/affymetrix/cran/versions.tsv diff --git a/workers/R/dependencies/affymetrix/install_affy_only.R b/workers/R/dependencies/affymetrix/install_affy_only.R new file mode 100644 index 000000000..2bce94bc4 --- /dev/null +++ b/workers/R/dependencies/affymetrix/install_affy_only.R @@ -0,0 +1,38 @@ +# Turn warnings into errors because biocLite throws warnings instead +# of error if it fails to install something. +options(warn = 2) +options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) +options(Ncpus = parallel::detectCores()) + +# Use devtools::install_version() to install packages in cran. +devtools::install_version("dplyr", version = "1.0.0") +devtools::install_version("tidyr", version = "1.1.0") +devtools::install_version("ff", version = "2.2-14") +devtools::install_version("locfit", version = "1.5-9.4") + +# Helper function that installs a list of packages using the input URLs +install_with_url <- function(urls) { + pkg_ids <- devtools::install_url(urls) + if (any(is.na(pkg_ids))) { + pkg_fails <- paste(urls[is.na(pkg_ids)], collapse = "; ") + stop(paste("Failed to install package(s):", pkg_fails)) + } + return(pkg_ids) +} + +bioc_pkgs <- c( + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/GEOquery_2.56.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/SCAN.UPC_2.30.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/affy_1.66.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/genefilter_1.70.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/sva_3.36.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/tximport_1.16.1.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz" +) +install_with_url(bioc_pkgs) diff --git a/workers/install_ensg_pkgs.R b/workers/R/dependencies/affymetrix/install_ensg_pkgs.R similarity index 89% rename from workers/install_ensg_pkgs.R rename to workers/R/dependencies/affymetrix/install_ensg_pkgs.R index 3f3b5bf08..5de25a262 100644 --- a/workers/install_ensg_pkgs.R +++ b/workers/R/dependencies/affymetrix/install_ensg_pkgs.R @@ -1,4 +1,4 @@ -options(Ncpus=parallel::detectCores()) +options(Ncpus = parallel::detectCores()) install.packages("xml2") library("xml2") ensg_url <- "http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/22.0.0/ensg.asp" @@ -39,8 +39,10 @@ lapply(data_rows, save_chip_pkg) # Write chips and pkg_urls to a tab-delimited file output_filename <- "/home/user/r_ensg_probe_pkgs.txt" -write.table(list(chips, pkg_urls), file=output_filename, quote=FALSE, - row.names=FALSE, col.names=FALSE, sep="\t") +write.table(list(chips, pkg_urls), + file = output_filename, quote = FALSE, + row.names = FALSE, col.names = FALSE, sep = "\t" +) # Install these ensg packages lapply(pkg_urls, devtools::install_url) diff --git a/workers/illumina_dependencies.R b/workers/R/dependencies/illumina/bioc/dependencies.R similarity index 100% rename from workers/illumina_dependencies.R rename to workers/R/dependencies/illumina/bioc/dependencies.R diff --git a/workers/R_dependencies/illumina/dependencies.R b/workers/R/dependencies/illumina/cran/dependencies.R similarity index 100% rename from workers/R_dependencies/illumina/dependencies.R rename to workers/R/dependencies/illumina/cran/dependencies.R diff --git a/workers/R_dependencies/illumina/packages.txt b/workers/R/dependencies/illumina/cran/packages.txt similarity index 100% rename from workers/R_dependencies/illumina/packages.txt rename to workers/R/dependencies/illumina/cran/packages.txt diff --git a/workers/R_dependencies/illumina/versions.tsv b/workers/R/dependencies/illumina/cran/versions.tsv similarity index 100% rename from workers/R_dependencies/illumina/versions.tsv rename to workers/R/dependencies/illumina/cran/versions.tsv diff --git a/workers/install_bioc.R b/workers/R/dependencies/install_bioc.R similarity index 58% rename from workers/install_bioc.R rename to workers/R/dependencies/install_bioc.R index c51d36988..3eb5aa29a 100644 --- a/workers/install_bioc.R +++ b/workers/R/dependencies/install_bioc.R @@ -1,12 +1,12 @@ # Turn warnings into errors because biocLite throws warnings instead # of error if it fails to install something. -options(warn=2) -options(repos=structure(c(CRAN="https://cloud.r-project.org"))) -options(Ncpus=parallel::detectCores()) +options(warn = 2) +options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) +options(Ncpus = parallel::detectCores()) # Use devtools::install_version() to install packages in cran. -devtools::install_version('dplyr', version='1.0.0') -devtools::install_version('tidyr', version='1.1.0') +devtools::install_version("dplyr", version = "1.0.0") +devtools::install_version("tidyr", version = "1.1.0") # devtools::install_url() requires BiocInstaller # install.packages('https://bioconductor.org/packages/3.6/bioc/src/contrib/BiocInstaller_1.28.0.tar.gz') diff --git a/workers/install_downloader_R_only.R b/workers/R/dependencies/install_downloader_R_only.R similarity index 54% rename from workers/install_downloader_R_only.R rename to workers/R/dependencies/install_downloader_R_only.R index 02feb6275..85eb866b1 100644 --- a/workers/install_downloader_R_only.R +++ b/workers/R/dependencies/install_downloader_R_only.R @@ -1,8 +1,8 @@ # Turn warnings into errors because biocLite throws warnings instead # of error if it fails to install something. -options(warn=2) -options(repos=structure(c(CRAN="https://cloud.r-project.org"))) -options(Ncpus=parallel::detectCores()) +options(warn = 2) +options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) +options(Ncpus = parallel::detectCores()) # Bioconductor packages, installed by devtools::install_url() @@ -10,15 +10,15 @@ options(Ncpus=parallel::detectCores()) # Helper function that installs a list of packages using the input URLs install_with_url <- function(urls) { pkg_ids <- devtools::install_url(urls) - if(any(is.na(pkg_ids))) { + if (any(is.na(pkg_ids))) { pkg_fails <- paste(urls[is.na(pkg_ids)], collapse = "; ") - stop(paste("Failed to install package(s):", pkg_fails )) + stop(paste("Failed to install package(s):", pkg_fails)) } return(pkg_ids) } bioc_pkgs <- c( - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz' + "https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz" ) install_with_url(bioc_pkgs) diff --git a/workers/R_dependencies/no_op/dependencies.R b/workers/R/dependencies/no_op/cran/dependencies.R similarity index 100% rename from workers/R_dependencies/no_op/dependencies.R rename to workers/R/dependencies/no_op/cran/dependencies.R diff --git a/workers/R_dependencies/no_op/packages.txt b/workers/R/dependencies/no_op/cran/packages.txt similarity index 100% rename from workers/R_dependencies/no_op/packages.txt rename to workers/R/dependencies/no_op/cran/packages.txt diff --git a/workers/R_dependencies/no_op/versions.tsv b/workers/R/dependencies/no_op/cran/versions.tsv similarity index 100% rename from workers/R_dependencies/no_op/versions.tsv rename to workers/R/dependencies/no_op/cran/versions.tsv diff --git a/workers/R/dependencies/no_op/install_gene_convert.R b/workers/R/dependencies/no_op/install_gene_convert.R new file mode 100644 index 000000000..2ad4afb84 --- /dev/null +++ b/workers/R/dependencies/no_op/install_gene_convert.R @@ -0,0 +1,43 @@ +# Turn warnings into errors because biocLite throws warnings instead +# of error if it fails to install something. +options(warn = 2) +options(Ncpus = parallel::detectCores()) +options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) + +# Helper function that installs a list of packages using the input URLs +install_with_url <- function(urls) { + pkg_ids <- devtools::install_url(urls) + if (any(is.na(pkg_ids))) { + pkg_fails <- paste(urls[is.na(pkg_ids)], collapse = "; ") + stop(paste("Failed to install package(s):", pkg_fails)) + } + return(pkg_ids) +} + +devtools::install_version("dplyr", version = "1.0.2") + +bioc_pkgs <- c( + "https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz" +) +install_with_url(bioc_pkgs) + +illumina_pkgs <- c( + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv1.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv2.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv3.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv4.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev1.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev1p1.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev2.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaRatv1.db_1.26.0.tar.gz" +) +install_with_url(illumina_pkgs) + +# Load these libraries because apparently just installing them isn't +# enough to verify that they have complementary versions. +library("optparse") +library(data.table) +library("dplyr") +library("rlang") +library(lazyeval) +library(AnnotationDbi) diff --git a/workers/qn_dependencies.R b/workers/R/dependencies/qn/bioc/dependencies.R similarity index 61% rename from workers/qn_dependencies.R rename to workers/R/dependencies/qn/bioc/dependencies.R index 8238bb7a4..2abd45f49 100644 --- a/workers/qn_dependencies.R +++ b/workers/R/dependencies/qn/bioc/dependencies.R @@ -1,19 +1,19 @@ -options(warn=2) -options(repos=structure(c(CRAN="https://cloud.r-project.org"))) -options(Ncpus=parallel::detectCores()) +options(warn = 2) +options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) +options(Ncpus = parallel::detectCores()) # Helper function that installs a list of packages using the input URLs install_with_url <- function(urls) { pkg_ids <- devtools::install_url(urls) - if(any(is.na(pkg_ids))) { + if (any(is.na(pkg_ids))) { pkg_fails <- paste(urls[is.na(pkg_ids)], collapse = "; ") - stop(paste("Failed to install package(s):", pkg_fails )) + stop(paste("Failed to install package(s):", pkg_fails)) } return(pkg_ids) } bioc_pkgs <- c( - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz' + "https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz" ) install_with_url(bioc_pkgs) diff --git a/workers/R_dependencies/qn/dependencies.R b/workers/R/dependencies/qn/cran/dependencies.R similarity index 100% rename from workers/R_dependencies/qn/dependencies.R rename to workers/R/dependencies/qn/cran/dependencies.R diff --git a/workers/R_dependencies/qn/packages.txt b/workers/R/dependencies/qn/cran/packages.txt similarity index 100% rename from workers/R_dependencies/qn/packages.txt rename to workers/R/dependencies/qn/cran/packages.txt diff --git a/workers/R_dependencies/qn/versions.tsv b/workers/R/dependencies/qn/cran/versions.tsv similarity index 100% rename from workers/R_dependencies/qn/versions.tsv rename to workers/R/dependencies/qn/cran/versions.tsv diff --git a/workers/R/dependencies/tximport/cran/dependencies.R b/workers/R/dependencies/tximport/cran/dependencies.R new file mode 100644 index 000000000..02c5ed011 --- /dev/null +++ b/workers/R/dependencies/tximport/cran/dependencies.R @@ -0,0 +1,25 @@ +# Generated from cranlock +options(warn = 2) +options(Ncpus = parallel::detectCores()) +options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) +devtools::install_version("getopt", version = "1.20.3") +devtools::install_version("optparse", version = "1.4.4") +devtools::install_version("rjson", version = "0.2.19") +devtools::install_version("R6", version = "2.4.0") +devtools::install_version("pkgconfig", version = "2.0.2") +devtools::install_version("rlang", version = "0.4.0") +devtools::install_version("zeallot", version = "0.1.0") +devtools::install_version("backports", version = "1.1.4") +devtools::install_version("glue", version = "1.3.1") +devtools::install_version("digest", version = "0.6.19") +devtools::install_version("vctrs", version = "0.1.0") +devtools::install_version("hms", version = "0.4.2") +devtools::install_version("Rcpp", version = "1.0.1") +devtools::install_version("assertthat", version = "0.2.1") +devtools::install_version("crayon", version = "1.3.4") +devtools::install_version("cli", version = "1.1.0") +devtools::install_version("utf8", version = "1.1.4") +devtools::install_version("fansi", version = "0.4.0") +devtools::install_version("pillar", version = "1.4.2") +devtools::install_version("tibble", version = "2.1.3") +devtools::install_version("readr", version = "1.1.1") diff --git a/workers/R_dependencies/tximport/packages.txt b/workers/R/dependencies/tximport/cran/packages.txt similarity index 100% rename from workers/R_dependencies/tximport/packages.txt rename to workers/R/dependencies/tximport/cran/packages.txt diff --git a/workers/R_dependencies/tximport/versions.tsv b/workers/R/dependencies/tximport/cran/versions.tsv similarity index 100% rename from workers/R_dependencies/tximport/versions.tsv rename to workers/R/dependencies/tximport/cran/versions.tsv diff --git a/workers/R/dependencies/tximport/install_tximport.R b/workers/R/dependencies/tximport/install_tximport.R new file mode 100644 index 000000000..f28c97538 --- /dev/null +++ b/workers/R/dependencies/tximport/install_tximport.R @@ -0,0 +1,7 @@ +# Turn warnings into errors because biocLite throws warnings instead +# of error if it fails to install something. +options(warn = 2) +options(Ncpus = parallel::detectCores()) +options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) + +devtools::install_url("https://bioconductor.org/packages/3.11/bioc/src/contrib/tximport_1.16.1.tar.gz") diff --git a/workers/R_dependencies/tximport/dependencies.R b/workers/R_dependencies/tximport/dependencies.R deleted file mode 100644 index 62fce5df5..000000000 --- a/workers/R_dependencies/tximport/dependencies.R +++ /dev/null @@ -1,25 +0,0 @@ -# Generated from cranlock -options(warn=2) -options(Ncpus=parallel::detectCores()) -options(repos=structure(c(CRAN="https://cloud.r-project.org"))) -devtools::install_version('getopt', version='1.20.3') -devtools::install_version('optparse', version='1.4.4') -devtools::install_version('rjson', version='0.2.19') -devtools::install_version('R6', version='2.4.0') -devtools::install_version('pkgconfig', version='2.0.2') -devtools::install_version('rlang', version='0.4.0') -devtools::install_version('zeallot', version='0.1.0') -devtools::install_version('backports', version='1.1.4') -devtools::install_version('glue', version='1.3.1') -devtools::install_version('digest', version='0.6.19') -devtools::install_version('vctrs', version='0.1.0') -devtools::install_version('hms', version='0.4.2') -devtools::install_version('Rcpp', version='1.0.1') -devtools::install_version('assertthat', version='0.2.1') -devtools::install_version('crayon', version='1.3.4') -devtools::install_version('cli', version='1.1.0') -devtools::install_version('utf8', version='1.1.4') -devtools::install_version('fansi', version='0.4.0') -devtools::install_version('pillar', version='1.4.2') -devtools::install_version('tibble', version='2.1.3') -devtools::install_version('readr', version='1.1.1') diff --git a/workers/affymetrix_dependencies.R b/workers/affymetrix_dependencies.R deleted file mode 100644 index 5fd501002..000000000 --- a/workers/affymetrix_dependencies.R +++ /dev/null @@ -1,220 +0,0 @@ -# Turn warnings into errors because biocLite throws warnings instead # of error if it fails to install something. -options(warn=2) -options(repos=structure(c(CRAN="https://cloud.r-project.org"))) -options(Ncpus=parallel::detectCores()) - -# Bioconductor packages, installed by devtools::install_url() - -# Helper function that installs a list of packages using the input URLs -install_with_url <- function(urls) { - pkg_ids <- devtools::install_url(urls) - if(any(is.na(pkg_ids))) { - pkg_fails <- paste(urls[is.na(pkg_ids)], collapse = "; ") - stop(paste("Failed to install package(s):", pkg_fails )) - } - return(pkg_ids) -} - -devtools::install_version('dplyr', version='1.0.2') - -bioc_pkg_urls <- c( - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/GEOquery_2.56.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/SCAN.UPC_2.30.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/affy_1.66.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/genefilter_1.70.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/sva_3.36.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz' -) -install_with_url(bioc_pkg_urls) - -# Invoke another R script to install BrainArray ensg packages -source("install_ensg_pkgs.R") - -# Install Bioconductor platform design (pd) packages -pd_experiment_pkgs <- c( - 'https://bioconductor.org/packages/3.11/data/experiment/src/contrib/pd.atdschip.tiling_0.26.0.tar.gz' -) -install_with_url(pd_experiment_pkgs) - -pd_annotation_pkgs <- c( - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.081229.hg18.promoter.medip.hx1_0.99.4.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.2006.07.18.hg18.refseq.promoter_1.8.1.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.2006.07.18.mm8.refseq.promoter_0.99.3.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.2006.10.31.rn34.refseq.promoter_0.99.3.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.ag_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.aragene.1.0.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.aragene.1.1.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.ath1.121501_3.12.0.tar.gz', - 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'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.clariom.s.human.ht_3.14.1.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.clariom.s.mouse_3.14.1.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.clariom.s.mouse.ht_3.14.1.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.clariom.s.rat_3.14.1.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.clariom.s.rat.ht_3.14.1.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cotton_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cyngene.1.0.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cyngene.1.1.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.cyrgene.1.0.st_3.12.0.tar.gz', - 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'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rjpgene.1.1.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rn.u34_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rta.1.0_3.12.2.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rusgene.1.0.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.rusgene.1.1.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.s.aureus_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.soybean_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.soygene.1.0.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.soygene.1.1.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.sugar.cane_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.tomato_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.u133.x3p_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.vitis.vinifera_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.wheat_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.x.laevis.2_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.x.tropicalis_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.xenopus.laevis_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.yeast.2_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.yg.s98_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.zebgene.1.0.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.zebgene.1.1.st_3.12.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/pd.zebrafish_3.12.0.tar.gz' -) -install_with_url(pd_annotation_pkgs) - -# Load this libraries because apparently just installing it isn't -# enough to verify that the correct versions of dependencies are installed. -library('foreach') diff --git a/workers/ccache.conf b/workers/ccache.conf new file mode 100644 index 000000000..cde43e665 --- /dev/null +++ b/workers/ccache.conf @@ -0,0 +1,5 @@ +max_size = 5.0G +# important for R CMD INSTALL *.tar.gz as tarballs are expanded freshly -> fresh ctime +sloppiness = include_file_ctime +# also important as the (temp.) directory name will differ +hash_dir = false diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index 151473ecf..3ee59ea1a 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -1,81 +1,30 @@ -FROM ubuntu:20.04 +FROM ccdlstaging/dr_worker_base:latest -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - -RUN apt-get update -qq -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable - -RUN apt-get update -qq -RUN apt-get install -y apt-fast apt-transport-https - -# The packages related to R are somewhat weird, see the README for more details. -COPY workers/CRAN.gpg . -RUN apt-key add CRAN.gpg -RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list - -RUN apt-fast update -qq && apt-fast install -y \ - build-essential \ - cmake \ - curl \ - cython3 \ - ed \ - git \ - libcairo-dev \ - libcurl4-openssl-dev \ - libedit-dev \ - libpq-dev \ - libssl-dev \ - libxml2-dev \ - llvm-10-dev \ - lsb-release \ - mercurial \ - pkg-config \ - python3-pip \ - python3 \ - python3-dev \ - r-base-core \ - wget - -RUN rm CRAN.gpg -RUN apt-get clean; rm -rf /var/lib/apt/lists/* -RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -ENV R_LIBS "/usr/local/lib/R/site-library" - -COPY common/install_devtools.R . -RUN Rscript install_devtools.R - -COPY workers/R_dependencies/affymetrix/dependencies.R . -RUN Rscript dependencies.R - -COPY workers/affymetrix_dependencies.R . -RUN Rscript affymetrix_dependencies.R +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip setuptools && \ + # Install this one here instead of via requirements.txt because not + # all processors need it. + pip3 install rpy2==3.4.5 -RUN pip3 install pip --upgrade -RUN pip3 install setuptools --upgrade +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) -# Install this one here instead of via requirements.txt because not -# all processors need it. -RUN pip3 install rpy2==3.4.5 COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +COPY workers/R/dependencies/affymetrix/cran/dependencies.R dependencies.R +RUN Rscript dependencies.R + +COPY workers/R/dependencies/affymetrix/install_ensg_pkgs.R . -# Clear out the pip3 cache. -RUN rm -rf /root/.cache +COPY workers/R/dependencies/affymetrix/bioc/dependencies.R dependencies.R +RUN Rscript dependencies.R ARG SYSTEM_VERSION @@ -86,6 +35,7 @@ USER user COPY .boto .boto COPY config/ config/ COPY workers/ . -COPY workers/install_ensg_pkgs.R . + +RUN ccache -s ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.affymetrix_local b/workers/dockerfiles/Dockerfile.affymetrix_local index 9a37692e6..3d7eff18f 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix_local +++ b/workers/dockerfiles/Dockerfile.affymetrix_local @@ -1,14 +1,18 @@ FROM ccdlstaging/dr_affymetrix:latest -USER root +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] + +WORKDIR /home/user # Remove the version of common already installed. -RUN rm -r common/ -RUN pip3 uninstall -y data_refinery_common +RUN rm -r common && \ + pip3 uninstall -y data_refinery_common # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) ARG SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 2c6a38784..82e14df01 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -3,102 +3,98 @@ FROM nvidia/cuda:11.8.0-runtime-ubuntu18.04 # This is very similar to the `smasher` image, but comes with OpenBLAS and some # of the other libraries required for fancyimpute. +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] + +WORKDIR /home/user + # Prevent tzdata from prompting us for a timezone and hanging the build. ENV DEBIAN_FRONTEND=noninteractive # Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 # For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -# RUN apt-key adv --fetch-keys https://developer.download.nvidia.com/compute/cuda/repos/ubuntu1804/x86_64/3bf863cc.pub -# via https://github.com/ilikenwf/apt-fast/issues/85#issuecomment-261640099 -RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections -RUN echo debconf apt-fast/dlflag boolean true | debconf-set-selections -RUN echo debconf apt-fast/aptmanager string apt-get | debconf-set-selections -RUN echo 'tzdata tzdata/Areas select Etc' | debconf-set-selections -RUN echo 'tzdata tzdata/Zones/Etc select UTC' | debconf-set-selections - -RUN apt-get update -qq -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN add-apt-repository ppa:deadsnakes/ppa -RUN add-apt-repository ppa:savoury1/llvm-defaults-10 - -RUN apt-get update -qq -RUN apt-get install -y apt-fast apt-transport-https tzdata +ENV LANG=C.UTF-8 COPY workers/CRAN.gpg . -RUN apt-key add CRAN.gpg -RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list - -RUN apt-fast update -qq && apt-fast install -y \ - build-essential \ - cmake \ - curl \ - cython3 \ - ed \ - gfortran \ - git \ - libcairo-dev \ - libcurl4-openssl-dev \ - libedit-dev \ - libblas-dev \ - liblapack-dev \ - libpq-dev \ - libssl-dev \ - libxml2-dev \ - llvm-10-dev \ - lsb-release \ - mercurial \ - pkg-config \ - python3-pip \ - python3.8 \ - python3.8-dev \ - r-base-core \ - wget - -RUN rm CRAN.gpg -RUN apt-get clean; rm -rf /var/lib/apt/lists/* -RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config -RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user -WORKDIR /home/user - -RUN wget https://bitbucket.org/ariya/phantomjs/downloads/phantomjs-2.1.1-linux-x86_64.tar.bz2 -RUN tar xvjf phantomjs-2.1.1-linux-x86_64.tar.bz2 -C /usr/local/share/ -RUN ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs /usr/local/bin/ -# We need a few special packages for QN -ENV R_LIBS "/usr/local/lib/R/site-library" - -COPY common/install_devtools.R . +# RUN apt-key adv --fetch-keys https://developer.download.nvidia.com/compute/cuda/repos/ubuntu1804/x86_64/3bf863cc.pub +# via https://github.com/ilikenwf/apt-fast/issues/85#issuecomment-261640099 +RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ + echo debconf apt-fast/dlflag boolean true | debconf-set-selections && \ + echo debconf apt-fast/aptmanager string apt-get | debconf-set-selections && \ + echo 'tzdata tzdata/Areas select Etc' | debconf-set-selections && \ + echo 'tzdata tzdata/Zones/Etc select UTC' | debconf-set-selections && \ + apt-get update -qq && \ + apt-get install --no-install-recommends -y software-properties-common && \ + add-apt-repository ppa:apt-fast/stable && \ + add-apt-repository ppa:deadsnakes/ppa && \ + add-apt-repository ppa:savoury1/llvm-defaults-10 && \ + apt-get update -qq && \ + apt-get install --no-install-recommends -y apt-fast apt-transport-https gpg-agent tzdata && \ + apt-key add CRAN.gpg && \ + echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list && \ + apt-fast update -qq && \ + apt-fast install -y \ + build-essential \ + ccache \ + cmake \ + curl \ + cython3 \ + ed \ + gfortran \ + git \ + libblas-dev \ + libcairo-dev \ + libcurl4-openssl-dev \ + libedit-dev \ + liblapack-dev \ + libpq-dev \ + libssl-dev \ + libxml2-dev \ + llvm-10-dev \ + lsb-release \ + mercurial \ + pkg-config \ + python3-pip \ + python3.8 \ + python3.8-dev \ + r-base-core \ + wget && \ + rm CRAN.gpg && \ + apt-get clean; rm -rf /var/lib/apt/lists/* && \ + ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config & \ + update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 && \ + groupadd user && useradd --create-home --home-dir /home/user -g user user && \ + wget -q https://bitbucket.org/ariya/phantomjs/downloads/phantomjs-2.1.1-linux-x86_64.tar.bz2 && \ + tar xvjf phantomjs-2.1.1-linux-x86_64.tar.bz2 -C /usr/local/share/ && \ + ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs /usr/local/bin/ + +# We need a few special packages for QN. +ENV R_LIBS="/usr/local/lib/R/site-library" + +COPY common/R/install_devtools.R . RUN Rscript install_devtools.R -COPY workers/R_dependencies/qn/dependencies.R . +COPY workers/R/dependencies/qn/cran/dependencies.R . RUN Rscript dependencies.R -COPY workers/qn_dependencies.R . -RUN Rscript qn_dependencies.R +COPY workers/R/dependencies/qn/bioc/dependencies.R . +RUN Rscript dependencies.R # End QN-specific -RUN pip3 install --upgrade pip -# Smasher-specific requirements -RUN pip3 install --ignore-installed numpy scipy matplotlib pandas==0.25.3 scikit-learn sympy nose rpy2===3.4.5 tzlocal fancySVD -# End smasher-specific - -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt - -RUN pip3 install --ignore-installed numpy==1.16.0 # Fix a downgrade - # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) -# Clear out the pip3 cache. -RUN rm -rf /root/.cache +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir numpy scipy matplotlib \ + pandas==0.25.3 scikit-learn sympy nose rpy2===3.4.5 tzlocal fancySVD + +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ + pip3 install --ignore-installed --no-cache-dir numpy==1.16.0 ARG SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 1b3337325..3a5974491 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -1,64 +1,11 @@ -FROM ubuntu:18.04 +FROM ccdlstaging/dr_worker_base:latest -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -RUN apt-get update -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN add-apt-repository ppa:deadsnakes/ppa -RUN add-apt-repository ppa:savoury1/llvm-defaults-10 - -RUN apt-get update -qq -RUN apt-get install -y apt-fast apt-transport-https - -# The packages related to R are somewhat weird, see the README for more details. -COPY workers/CRAN.gpg . -RUN apt-key add CRAN.gpg -RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list - -RUN apt-fast update -qq && apt-fast install -y \ - build-essential \ - cmake \ - curl \ - cython3 \ - ed \ - git \ - libcairo-dev \ - libcurl4-openssl-dev \ - libedit-dev \ - libpq-dev \ - libssl-dev \ - libxml2-dev \ - llvm-10-dev \ - lsb-release \ - mercurial \ - pkg-config \ - python3-pip \ - python3.8 \ - python3.8-dev \ - r-base-core \ - wget - -RUN rm CRAN.gpg -RUN apt-get clean; rm -rf /var/lib/apt/lists/* -RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config -RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -ENV R_LIBS "/usr/local/lib/R/site-library" - -COPY common/install_devtools.R . -RUN Rscript install_devtools.R - -COPY workers/install_downloader_R_only.R . +COPY workers/R/dependencies/install_downloader_R_only.R . RUN Rscript install_downloader_R_only.R # Aspera will only install as the current user. @@ -67,29 +14,25 @@ USER user # Install Aspera. We have to install it using Holland Computing Center's conda # repo because download.asperasoft.com now returns 403s -RUN wget -q https://anaconda.org/HCC/aspera-cli/3.9.1/download/linux-64/aspera-cli-3.9.1-0.tar.bz2 -RUN [ "$(sha256sum aspera-cli-3.9.1-0.tar.bz2 | cut -d' ' -f1)" = 60a09a7f3795186954079869106aa89a64183b7be8e0da7cbbe9d57c66c9bcdb ] -RUN mkdir -p .aspera/cli -RUN tar xf aspera-cli-3.9.1-0.tar.bz2 -C .aspera/cli -RUN rm aspera-cli-3.9.1-0.tar.bz2 +RUN wget -q https://anaconda.org/HCC/aspera-cli/3.9.1/download/linux-64/aspera-cli-3.9.1-0.tar.bz2 && \ + [ "$(sha256sum aspera-cli-3.9.1-0.tar.bz2 | cut -d ' ' -f1)" = 60a09a7f3795186954079869106aa89a64183b7be8e0da7cbbe9d57c66c9bcdb ] && \ + mkdir -p .aspera/cli && \ + tar xf aspera-cli-3.9.1-0.tar.bz2 -C .aspera/cli && \ + rm aspera-cli-3.9.1-0.tar.bz2 # Now that we're done installing Aspera go back to being root for a bit. USER root -RUN pip3 install --upgrade pip -# Install this rpy2 here instead of via requirements.txt because -# pip-compile throws an error for it. -RUN pip3 install rpy2==3.4.5 - -COPY workers/data_refinery_workers/downloaders/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir rpy2==3.4.5 # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) -# Clear out the pip3 cache. -RUN rm -rf /root/.cache +COPY workers/data_refinery_workers/downloaders/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt ARG SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index e4cc70268..949c22405 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -1,83 +1,25 @@ -FROM ubuntu:18.04 +FROM ccdlstaging/dr_worker_base:latest -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -RUN apt-get update -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN add-apt-repository ppa:deadsnakes/ppa -RUN add-apt-repository ppa:savoury1/llvm-defaults-10 - -RUN apt-get update -qq -RUN apt-get install -y apt-fast apt-transport-https - -# The packages related to R are somewhat weird, see the README for more details. -COPY workers/CRAN.gpg . -RUN apt-key add CRAN.gpg -RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list - -RUN apt-fast update -qq && apt-fast install -y \ - build-essential \ - cmake \ - curl \ - cython3 \ - ed \ - gfortran \ - git \ - libcairo-dev \ - libcurl4-openssl-dev \ - libedit-dev \ - libblas-dev \ - liblapack-dev \ - libpq-dev \ - libssl-dev \ - libxml2-dev \ - llvm-10-dev \ - lsb-release \ - mercurial \ - pkg-config \ - python3-pip \ - python3.8 \ - python3.8-dev \ - r-base-core \ - wget - -RUN rm CRAN.gpg -RUN apt-get clean; rm -rf /var/lib/apt/lists/* -RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config -RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -ENV R_LIBS "/usr/local/lib/R/site-library" - -COPY common/install_devtools.R . -RUN Rscript install_devtools.R - -COPY workers/R_dependencies/illumina/dependencies.R . +COPY workers/R/dependencies/illumina/cran/dependencies.R . RUN Rscript dependencies.R # These are for Illumina. -COPY workers/illumina_dependencies.R . -RUN Rscript illumina_dependencies.R - -RUN pip3 install --upgrade pip -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt +COPY workers/R/dependencies/illumina/bioc/dependencies.R . +RUN Rscript dependencies.R # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) -# Clear out the pip3 cache. -RUN rm -rf /root/.cache +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed -r requirements.txt ARG SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index 98f35d772..a0cb7855e 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -1,94 +1,38 @@ -FROM ubuntu:18.04 +FROM ccdlstaging/dr_worker_base:latest -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -RUN apt-get update -qq -RUN apt-get install -y software-properties-common - -RUN add-apt-repository ppa:apt-fast/stable -# deadsnakes packages new python versions for older Ubuntu releases -RUN add-apt-repository ppa:deadsnakes/ppa - -RUN apt-get update -qq -RUN apt-get -y install apt-fast - -# The packages related to R are somewhat weird, see the README for more details. -COPY workers/CRAN.gpg . -RUN apt-key add CRAN.gpg -RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list - -RUN apt-fast update -qq && apt-fast install -y \ - build-essential \ - cmake \ - curl \ - cython3 \ - ed \ - git \ - libcairo-dev \ - libcurl4-openssl-dev \ - libedit-dev \ - libfreetype6-dev \ - libpq-dev \ - libssl-dev \ - libxml2-dev \ - llvm-10-dev \ - lsb-release \ - mercurial \ - pkg-config \ - python3-pip \ - python3.8 \ - python3.8-dev \ - r-base-core \ - wget - -RUN rm CRAN.gpg -RUN apt-get clean; rm -rf /var/lib/apt/lists/* -RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config -RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -# Noop-specific requirements -ENV R_LIBS "/usr/local/lib/R/site-library" - -COPY common/install_devtools.R . -RUN Rscript install_devtools.R - -COPY workers/R_dependencies/no_op/dependencies.R . +COPY workers/R/dependencies/no_op/cran/dependencies.R . RUN Rscript dependencies.R -COPY workers/install_gene_convert.R . +COPY workers/R/dependencies/no_op/install_gene_convert.R . RUN Rscript install_gene_convert.R +# Noop-specific. RUN mkdir -p gene_indexes WORKDIR /home/user/gene_indexes ENV ID_REFINERY_URL https://zenodo.org/record/1410647/files/all_1536267482.zip -RUN curl -O $ID_REFINERY_URL -RUN echo $ID_REFINERY_URL > /etc/identifier_refinery_url -RUN unzip *.zip -RUN rm *.zip +RUN curl -O $ID_REFINERY_URL && \ + echo $ID_REFINERY_URL > /etc/identifier_refinery_url && \ + unzip *.zip && \ + rm *.zip +# End Noop-specific. + WORKDIR /home/user -# End Noop-specific -RUN pip3 install --upgrade pip -RUN pip3 install numpy +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir numpy COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) - -# Clear out the pip3 cache. -RUN rm -rf /root/.cache +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) ARG SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index 8bfbbe9a7..724ddac60 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -1,109 +1,59 @@ -FROM ubuntu:18.04 - -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -RUN apt-get update -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN add-apt-repository ppa:deadsnakes/ppa -RUN add-apt-repository ppa:savoury1/llvm-defaults-10 - -RUN apt-get update -qq -RUN apt-get install -y apt-fast apt-transport-https - -# The packages related to R are somewhat weird, see the README for more details. -COPY workers/CRAN.gpg . -RUN apt-key add CRAN.gpg -RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list - -RUN apt-fast update -qq && apt-fast install -y \ - build-essential \ - cmake \ - curl \ - cython3 \ - ed \ - git \ - libcairo-dev \ - libcurl4-openssl-dev \ - libedit-dev \ - libpq-dev \ - libssl-dev \ - libxml2-dev \ - llvm-10-dev \ - lsb-release \ - mercurial \ - pkg-config \ - python3-pip \ - python3.8 \ - python3.8-dev \ - r-base-core \ - wget - -RUN rm CRAN.gpg -RUN apt-get upgrade; apt-get clean; rm -rf /var/lib/apt/lists/* -RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config -RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user +FROM ccdlstaging/dr_worker_base:latest + +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] + WORKDIR /home/user # Install Salmon - -# Tximport requires all experiments to be processed with the same version of Salmon to work https://github.com/AlexsLemonade/refinebio/issues/1496 +# Tximport requires all experiments to be processed with the same version of +# Salmon to work https://github.com/AlexsLemonade/refinebio/issues/1496. # This is something that should be considered when updating salmon, because # all samples from incomplete experiments must have salmon run on them again. ENV SALMON_VERSION 0.13.1 -RUN wget https://github.com/COMBINE-lab/salmon/releases/download/v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz -RUN mkdir Salmon-${SALMON_VERSION}_linux_x86_64 # On version 0.13.1 salmon was being extracted to a folder with an all lowercase name # the options `-C` and `--strip-components` allow us to specify the name for the resulting file -RUN tar -xzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz -C Salmon-${SALMON_VERSION}_linux_x86_64 --strip-components 1 -# Create soft link `/usr/local/bin/salmon` that points to the actual program -RUN ln -sf `pwd`/Salmon-${SALMON_VERSION}_linux_x86_64/bin/salmon /usr/local/bin/ -RUN rm -f Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz +RUN wget -q "https://github.com/COMBINE-lab/salmon/releases/download/v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ + mkdir "Salmon-${SALMON_VERSION}_linux_x86_64" && \ + tar -xzf "Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" \ + -C "Salmon-${SALMON_VERSION}_linux_x86_64" --strip-components 1 && \ + ln -sf "$(pwd)/Salmon-${SALMON_VERSION}_linux_x86_64/bin/salmon" \ + /usr/local/bin/ && \ + rm -f "Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" # End Salmon installation. -# Install R dependencies. -COPY common/install_devtools.R . -RUN Rscript install_devtools.R - -COPY workers/R_dependencies/tximport/dependencies.R tximport_dependencies.R +COPY workers/R/dependencies/tximport/cran/dependencies.R tximport_dependencies.R RUN Rscript tximport_dependencies.R # Install tximport. -COPY workers/install_tximport.R . +COPY workers/R/dependencies/tximport/install_tximport.R . RUN Rscript install_tximport.R -RUN pip3 install --upgrade pip -RUN pip3 install numpy - -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir numpy # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) + +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Install SalmonTools. -RUN git clone https://github.com/COMBINE-lab/SalmonTools.git && cd SalmonTools && git checkout 3e6654c2c10a5225498b623056993947fa688afc -RUN cd SalmonTools && cmake . -DCMAKE_INSTALL_PREFIX=/usr/local && make install -RUN rm -rf SalmonTools +RUN git clone https://github.com/COMBINE-lab/SalmonTools.git && \ + cd SalmonTools && \ + git checkout 3e6654c2c10a5225498b623056993947fa688afc && \ + cmake . -DCMAKE_INSTALL_PREFIX=/usr/local && \ + make install && \ + rm -rf SalmonTools # Install sra-tools. ENV SRA_VERSION 2.9.1 -RUN wget "https://ftp.ncbi.nlm.nih.gov/sra/sdk/${SRA_VERSION}/sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ - tar zxfv sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz && \ - cp -r sratoolkit.${SRA_VERSION}-ubuntu64/bin/* /usr/bin - -# Clear out the pip3 cache. -RUN rm -rf /root/.cache +RUN wget -q "https://ftp.ncbi.nlm.nih.gov/sra/sdk/${SRA_VERSION}/sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ + tar zxfv "sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ + cp -r "sratoolkit.${SRA_VERSION}-ubuntu64/bin/"* /usr/bin ARG SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index 313ba2150..00ea02baa 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -1,88 +1,26 @@ -FROM ubuntu:18.04 +FROM ccdlstaging/dr_worker_base:latest -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -RUN apt-get update -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN add-apt-repository ppa:deadsnakes/ppa -RUN add-apt-repository ppa:savoury1/llvm-defaults-10 - -RUN apt-get update -qq -RUN apt-get install -y apt-fast apt-transport-https - -# The packages related to R are somewhat weird, see the README for more details. -COPY workers/CRAN.gpg . -RUN apt-key add CRAN.gpg -RUN echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list - -RUN apt-fast update -qq && apt-fast install -y \ - build-essential \ - cmake \ - curl \ - cython3 \ - ed \ - gfortran \ - git \ - libcairo-dev \ - libcurl4-openssl-dev \ - libedit-dev \ - libblas-dev \ - liblapack-dev \ - libpq-dev \ - libssl-dev \ - libxml2-dev \ - llvm-10-dev \ - lsb-release \ - mercurial \ - pkg-config \ - python3-pip \ - python3.8 \ - python3.8-dev \ - r-base-core \ - wget - -RUN rm CRAN.gpg -RUN apt-get clean; rm -rf /var/lib/apt/lists/* -RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config -RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -# We need a few special packages for QN -ENV R_LIBS "/usr/local/lib/R/site-library" - -COPY common/install_devtools.R . -RUN Rscript install_devtools.R - -COPY workers/R_dependencies/qn/dependencies.R . -RUN Rscript dependencies.R - -COPY workers/qn_dependencies.R . -RUN Rscript qn_dependencies.R -# End QN-specific +RUN pip3 install --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir nose numpy rpy2==3.4.5 -RUN pip3 install --upgrade pip -# Smasher-specific requirements -RUN pip3 install --ignore-installed nose numpy rpy2==3.4.5 -# End smasher-specific +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +COPY workers/R/dependencies/qn/cran/dependencies.R . +RUN Rscript dependencies.R -# Clear out the pip3 cache. -RUN rm -rf /root/.cache +COPY workers/R/dependencies/qn/bioc/dependencies.R . +RUN Rscript dependencies.R ARG SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index d1ac0ea63..1c04e7390 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -1,87 +1,41 @@ -FROM ubuntu:18.04 +FROM ccdlstaging/dr_worker_base:latest -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -RUN apt-get update -qq -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN add-apt-repository ppa:deadsnakes/ppa -RUN add-apt-repository ppa:savoury1/llvm-defaults-10 - -RUN apt-get update -qq -RUN apt-get -y install apt-fast - -RUN apt-fast update -qq && apt-fast install -y \ - build-essential \ - curl \ - cython3 \ - ed \ - git \ - libcurl4-openssl-dev \ - libfreetype6-dev \ - libpq-dev \ - llvm-10-dev \ - pkg-config \ - python3-pip \ - python3.8 \ - python3.8-dev \ - wget \ - zlib1g-dev - -RUN rm CRAN.gpg -RUN apt-get clean; rm -rf /var/lib/apt/lists/* -RUN ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config -RUN update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user # It's annoying that this can only be installed via git. -RUN git clone https://github.com/deweylab/RSEM.git -RUN cd RSEM && make install -RUN rm -rf RSEM - -# Install Salmon +RUN git clone https://github.com/deweylab/RSEM.git && \ + cd RSEM && make install && \ + rm -rf RSEM -# Tximport requires all experiments to be processed with the same version of Salmon to work https://github.com/AlexsLemonade/refinebio/issues/1496 +# Install Salmon. +# Tximport requires all experiments to be processed with the same version of +# Salmon to work https://github.com/AlexsLemonade/refinebio/issues/1496. # This is something that should be considered when updating salmon, because # all samples from incomplete experiments must have salmon run on them again. ENV SALMON_VERSION 0.13.1 -# Doesn't work: -# salmon: relocation error: /usr/local/bin/../lib/librt.so.1: symbol __vdso_clock_gettime, version GLIBC_PRIVATE not defined in file libc.so.6 with link time reference -# ENV SALMON_VERSION 0.10.0 - -# Binary releases moved to bioconda, doesn't work anymore. -# ENV SALMON_VERSION 0.10.2 - -RUN wget https://github.com/COMBINE-lab/salmon/releases/download/v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz -RUN tar -xzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz - # Salmon can extract to a different directory than the name of the tar file. -RUN cp `tar -tzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz | head -1 | cut -f1 -d"/"`/bin/salmon /usr/local/bin -RUN cp `tar -tzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz | head -1 | cut -f1 -d"/"`/lib/* /usr/local/lib - -RUN rm -r Salmon* +RUN wget -q "https://github.com/COMBINE-lab/salmon/releases/download/v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ + tar -xzf "Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ + cp "$(tar -tzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz | \ + head -1 | cut -f1 -d '/')/bin/salmon" /usr/local/bin && \ + cp "$(tar -tzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz | \ + head -1 | cut -f1 -d '/')/lib/"* /usr/local/lib/ && \ + rm -r Salmon* # End Salmon installation. -RUN pip3 install --upgrade pip -RUN pip3 install numpy +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir numpy -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt - -# Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) -# Clear out the pip3 cache. -RUN rm -rf /root/.cache +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt ARG SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.worker_base b/workers/dockerfiles/Dockerfile.worker_base new file mode 100644 index 000000000..d8f1a0588 --- /dev/null +++ b/workers/dockerfiles/Dockerfile.worker_base @@ -0,0 +1,68 @@ +FROM ubuntu:18.04 + +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] + +WORKDIR /home/user + +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG=C.UTF-8 + +RUN apt-get update && \ + apt-get install --no-install-recommends -y software-properties-common && \ + add-apt-repository ppa:apt-fast/stable && \ + add-apt-repository ppa:deadsnakes/ppa && \ + add-apt-repository ppa:savoury1/llvm-defaults-10 && \ + apt-get update -qq && \ + apt-get install --no-install-recommends -y apt-fast apt-transport-https gpg-agent + +# The packages related to R are somewhat weird, see the README for more details. +COPY workers/CRAN.gpg . +RUN apt-key add CRAN.gpg && \ + echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list + +RUN apt-fast update -qq && apt-fast install -y \ + build-essential \ + ccache \ + cmake \ + curl \ + cython3 \ + ed \ + git \ + libcairo-dev \ + libcurl4-gnutls-dev \ + libedit-dev \ + libgit2-dev \ + libpq-dev \ + libssl-dev \ + libxml2-dev \ + llvm-10-dev \ + lsb-release \ + mercurial \ + pkg-config \ + python3-pip \ + python3.8 \ + python3.8-dev \ + r-base-core \ + wget && \ + rm CRAN.gpg && \ + apt-get clean && \ + rm -rf /var/lib/apt/lists/* && \ + ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ + update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 && \ + groupadd user && useradd --create-home --home-dir /home/user -g user user + +# Set up ccache. +COPY workers/ccache.conf /root/.ccache/ccache.conf +RUN for i in c++ cc g++ gcc; do ln -s /usr/bin/ccache /usr/local/bin/$i; done +ENV PATH="/usr/local/bin:${PATH}" + +# Pre-install dev tools. +ENV R_LIBS="/usr/local/lib/R/site-library" +COPY common/R/install_devtools.R . +RUN Rscript install_devtools.R diff --git a/workers/install_affy_only.R b/workers/install_affy_only.R deleted file mode 100644 index b488a0287..000000000 --- a/workers/install_affy_only.R +++ /dev/null @@ -1,38 +0,0 @@ -# Turn warnings into errors because biocLite throws warnings instead -# of error if it fails to install something. -options(warn=2) -options(repos=structure(c(CRAN="https://cloud.r-project.org"))) -options(Ncpus=parallel::detectCores()) - -# Use devtools::install_version() to install packages in cran. -devtools::install_version('dplyr', version='1.0.0') -devtools::install_version('tidyr', version='1.1.0') -devtools::install_version('ff', version='2.2-14') -devtools::install_version('locfit', version='1.5-9.4') - -# Helper function that installs a list of packages using the input URLs -install_with_url <- function(urls) { - pkg_ids <- devtools::install_url(urls) - if(any(is.na(pkg_ids))) { - pkg_fails <- paste(urls[is.na(pkg_ids)], collapse = "; ") - stop(paste("Failed to install package(s):", pkg_fails )) - } - return(pkg_ids) -} - -bioc_pkgs <- c( - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/GEOquery_2.56.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/SCAN.UPC_2.30.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/affy_1.66.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/genefilter_1.70.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/sva_3.36.0.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/tximport_1.16.1.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz' -) -install_with_url(bioc_pkgs) diff --git a/workers/install_gene_convert.R b/workers/install_gene_convert.R deleted file mode 100644 index 5e053f9c6..000000000 --- a/workers/install_gene_convert.R +++ /dev/null @@ -1,43 +0,0 @@ -# Turn warnings into errors because biocLite throws warnings instead -# of error if it fails to install something. -options(warn=2) -options(Ncpus=parallel::detectCores()) -options(repos=structure(c(CRAN="https://cloud.r-project.org"))) - -# Helper function that installs a list of packages using the input URLs -install_with_url <- function(urls) { - pkg_ids <- devtools::install_url(urls) - if(any(is.na(pkg_ids))) { - pkg_fails <- paste(urls[is.na(pkg_ids)], collapse = "; ") - stop(paste("Failed to install package(s):", pkg_fails )) - } - return(pkg_ids) -} - -devtools::install_version('dplyr', version='1.0.2') - -bioc_pkgs <- c( - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz' -) -install_with_url(bioc_pkgs) - -illumina_pkgs <- c( - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv1.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv2.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv3.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv4.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev1.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev1p1.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev2.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaRatv1.db_1.26.0.tar.gz' -) -install_with_url(illumina_pkgs) - -# Load these libraries because apparently just installing them isn't -# enough to verify that they have complementary versions. -library("optparse") -library(data.table) -library("dplyr") -library("rlang") -library(lazyeval) -library(AnnotationDbi) diff --git a/workers/install_tximport.R b/workers/install_tximport.R deleted file mode 100644 index 2889fc630..000000000 --- a/workers/install_tximport.R +++ /dev/null @@ -1,7 +0,0 @@ -# Turn warnings into errors because biocLite throws warnings instead -# of error if it fails to install something. -options(warn=2) -options(Ncpus=parallel::detectCores()) -options(repos=structure(c(CRAN="https://cloud.r-project.org"))) - -devtools::install_url('https://bioconductor.org/packages/3.11/bioc/src/contrib/tximport_1.16.1.tar.gz') From f2260eaa7f0033a1cdfa8a862ccdd34ea2c73fe6 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 17 Nov 2022 15:32:21 -0800 Subject: [PATCH 049/220] Update foreman Docker image. --- foreman/dockerfiles/Dockerfile.foreman | 55 ++++++++++++++------------ 1 file changed, 30 insertions(+), 25 deletions(-) diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 8b7b9f239..f95cb393e 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -1,35 +1,40 @@ FROM ubuntu:18.04 -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -RUN apt-get update -qq -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable - -RUN apt-get update -qq -RUN apt-get -y install apt-fast -RUN apt-fast update -qq -RUN apt-fast install -y \ - gcc \ - libpq-dev \ - python3 \ - python3-pip - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user -WORKDIR /home/user +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -RUN pip3 install --upgrade pip +WORKDIR /home/user -COPY foreman/requirements.txt . -# The base image does not have Python 2.X on it at all, so all calls -# to pip or python are by default calls to pip3 or python3 -RUN pip3 install -r requirements.txt +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG=C.UTF-8 + +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +RUN apt-get update -qq && \ + apt-get install --no-install-recommends -y software-properties-common && \ + add-apt-repository ppa:apt-fast/stable && \ + apt-get update -qq && \ + apt-get install --no-install-recommends -y apt-fast && \ + apt-fast update -qq && \ + apt-fast install -y \ + gcc \ + libpq-dev \ + python3 \ + python3-pip && \ + apt-get clean && \ + groupadd user && \ + useradd --create-home --home-dir /home/user -g user user && \ + pip3 install --ignore-installed --no-cache-dir --upgrade pip # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) + +COPY foreman/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt COPY .boto .boto COPY config config From 5f3b5625a0c13a72a9e191ff7c15255cb0fdd415 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 22 Nov 2022 13:45:21 -0800 Subject: [PATCH 050/220] Update docker images. --- common/dockerfiles/Dockerfile.base | 65 +++++++++++ .../dependencies/illumina/bioc/dependencies.R | 36 +++---- workers/dockerfiles/Dockerfile.affymetrix | 41 ++++--- .../dockerfiles/Dockerfile.affymetrix_local | 3 +- workers/dockerfiles/Dockerfile.compendia | 102 +++++++++--------- workers/dockerfiles/Dockerfile.downloaders | 23 ++-- workers/dockerfiles/Dockerfile.illumina | 12 ++- workers/dockerfiles/Dockerfile.no_op | 23 ++-- workers/dockerfiles/Dockerfile.salmon | 28 ++--- workers/dockerfiles/Dockerfile.smasher | 27 ++--- workers/dockerfiles/Dockerfile.transcriptome | 20 ++-- workers/dockerfiles/Dockerfile.worker_base | 68 ------------ 12 files changed, 238 insertions(+), 210 deletions(-) create mode 100644 common/dockerfiles/Dockerfile.base delete mode 100644 workers/dockerfiles/Dockerfile.worker_base diff --git a/common/dockerfiles/Dockerfile.base b/common/dockerfiles/Dockerfile.base new file mode 100644 index 000000000..5b833cef9 --- /dev/null +++ b/common/dockerfiles/Dockerfile.base @@ -0,0 +1,65 @@ +FROM ubuntu:18.04 + +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] + +WORKDIR /home/user + +# Prevent tzdata from prompting us for a timezone and hanging the build. +ENV DEBIAN_FRONTEND=noninteractive + +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG=C.UTF-8 + +COPY workers/CRAN.gpg . +RUN apt-get update && \ + apt-get install --no-install-recommends -y software-properties-common && \ + add-apt-repository ppa:apt-fast/stable && \ + add-apt-repository ppa:deadsnakes/ppa && \ + add-apt-repository ppa:savoury1/llvm-defaults-10 && \ + apt-get update -qq && \ + apt-get install --no-install-recommends -y \ + apt-fast \ + apt-transport-https \ + gpg-agent && \ + apt-key add CRAN.gpg && \ + echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ + >> /etc/apt/sources.list.d/added_repos.list && \ + apt-fast update -qq && apt-fast install -y \ + build-essential \ + cmake \ + curl \ + cython3 \ + ed \ + git \ + libcairo-dev \ + libcurl4-gnutls-dev \ + libedit-dev \ + libgit2-dev \ + libpq-dev \ + libssl-dev \ + libxml2-dev \ + llvm-10-dev \ + lsb-release \ + mercurial \ + pkg-config \ + python3-pip \ + python3.8 \ + python3.8-dev \ + r-base-core \ + wget && \ + apt-get clean && \ + rm CRAN.gpg && \ + rm -rf /var/lib/apt/lists/* && \ + ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ + update-alternatives --install \ + /usr/bin/python3 python3 /usr/bin/python3.8 1 && \ + groupadd user && \ + useradd --create-home --home-dir /home/user/ -g user user && \ + chown -R user /home/user/ + +# Pre-install dev tools. +ENV R_LIBS=/usr/local/lib/R/site-library +COPY common/R/install_devtools.R . +RUN Rscript install_devtools.R diff --git a/workers/R/dependencies/illumina/bioc/dependencies.R b/workers/R/dependencies/illumina/bioc/dependencies.R index b3e598f79..06aee98a5 100644 --- a/workers/R/dependencies/illumina/bioc/dependencies.R +++ b/workers/R/dependencies/illumina/bioc/dependencies.R @@ -1,36 +1,36 @@ -options(warn=2) -options(repos=structure(c(CRAN="https://cloud.r-project.org"))) -options(Ncpus=parallel::detectCores()) +options(warn = 2) +options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) +options(Ncpus = parallel::detectCores()) # Helper function that installs a list of packages using the input URLs install_with_url <- function(urls) { pkg_ids <- devtools::install_url(urls) - if(any(is.na(pkg_ids))) { + if (any(is.na(pkg_ids))) { pkg_fails <- paste(urls[is.na(pkg_ids)], collapse = "; ") - stop(paste("Failed to install package(s):", pkg_fails )) + stop(paste("Failed to install package(s):", pkg_fails)) } return(pkg_ids) } -devtools::install_version('dplyr', version='1.0.2') +devtools::install_version("dplyr", version = "1.0.2") bioc_pkgs <- c( - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz', - 'https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz' + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz" ) install_with_url(bioc_pkgs) illumina_pkgs <- c( - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv1.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv2.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv3.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv4.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev1.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev1p1.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev2.db_1.26.0.tar.gz', - 'https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaRatv1.db_1.26.0.tar.gz' + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv1.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv2.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv3.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaHumanv4.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev1.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev1p1.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaMousev2.db_1.26.0.tar.gz", + "https://bioconductor.org/packages/3.11/data/annotation/src/contrib/illuminaRatv1.db_1.26.0.tar.gz" ) install_with_url(illumina_pkgs) diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index 3ee59ea1a..e90300240 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -5,18 +5,12 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip setuptools && \ - # Install this one here instead of via requirements.txt because not - # all processors need it. - pip3 install rpy2==3.4.5 - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ - common/$(ls common -1 | sort --version-sort | tail -1) - -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt +# Install and set up ccache. +RUN apt-get update && \ + apt-get install --no-install-recommends -y ccache && \ + for i in c++ cc g++ gcc; do ln -fs /usr/bin/ccache /usr/local/bin/$i; done +COPY workers/ccache.conf /root/.ccache/ccache.conf +ENV PATH="/usr/local/bin:${PATH}" COPY workers/R/dependencies/affymetrix/cran/dependencies.R dependencies.R RUN Rscript dependencies.R @@ -26,9 +20,28 @@ COPY workers/R/dependencies/affymetrix/install_ensg_pkgs.R . COPY workers/R/dependencies/affymetrix/bioc/dependencies.R dependencies.R RUN Rscript dependencies.R +RUN pip3 install --ignore-installed --upgrade pip setuptools && \ + # Install this one here instead of via requirements.txt because not + # all processors need it. + pip3 install --ignore-installed rpy2==3.4.5 + +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed \ + common/$(ls common -1 | sort --version-sort | tail -1) + +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip3 cache. + rm -r /root/.cache + ARG SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION -ENV SYSTEM_VERSION $SYSTEM_VERSION +# Print compiler cache stats (the cache hit ratio should be ~90%). +RUN ccache --show-stats && \ + # Clear out the ccache. + ccache --clear USER user @@ -36,6 +49,4 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . -RUN ccache -s - ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.affymetrix_local b/workers/dockerfiles/Dockerfile.affymetrix_local index 3d7eff18f..7f1af8ad6 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix_local +++ b/workers/dockerfiles/Dockerfile.affymetrix_local @@ -15,8 +15,7 @@ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 82e14df01..f2afa185f 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -1,13 +1,11 @@ -FROM nvidia/cuda:11.8.0-runtime-ubuntu18.04 - # This is very similar to the `smasher` image, but comes with OpenBLAS and some # of the other libraries required for fancyimpute. +FROM nvidia/cuda:11.8.0-runtime-ubuntu18.04 + # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] -WORKDIR /home/user - # Prevent tzdata from prompting us for a timezone and hanging the build. ENV DEBIAN_FRONTEND=noninteractive @@ -33,45 +31,52 @@ RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ apt-get install --no-install-recommends -y apt-fast apt-transport-https gpg-agent tzdata && \ apt-key add CRAN.gpg && \ echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ + >> /etc/apt/sources.list.d/added_repos.list && \ apt-fast update -qq && \ apt-fast install -y \ - build-essential \ - ccache \ - cmake \ - curl \ - cython3 \ - ed \ - gfortran \ - git \ - libblas-dev \ - libcairo-dev \ - libcurl4-openssl-dev \ - libedit-dev \ - liblapack-dev \ - libpq-dev \ - libssl-dev \ - libxml2-dev \ - llvm-10-dev \ - lsb-release \ - mercurial \ - pkg-config \ - python3-pip \ - python3.8 \ - python3.8-dev \ - r-base-core \ - wget && \ + build-essential \ + ccache \ + cmake \ + curl \ + cython3 \ + ed \ + gfortran \ + git \ + libblas-dev \ + libcairo-dev \ + libcurl4-openssl-dev \ + libedit-dev \ + liblapack-dev \ + libpq-dev \ + libssl-dev \ + libxml2-dev \ + llvm-10-dev \ + lsb-release \ + mercurial \ + pkg-config \ + python3-pip \ + python3.8 \ + python3.8-dev \ + r-base-core \ + wget && \ + apt-get clean && \ rm CRAN.gpg && \ - apt-get clean; rm -rf /var/lib/apt/lists/* && \ - ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config & \ - update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 && \ - groupadd user && useradd --create-home --home-dir /home/user -g user user && \ - wget -q https://bitbucket.org/ariya/phantomjs/downloads/phantomjs-2.1.1-linux-x86_64.tar.bz2 && \ + rm -rf /var/lib/apt/lists/* && \ + ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ + update-alternatives --install /usr/bin/python3 python3 \ + /usr/bin/python3.8 1 && \ + groupadd user && \ + useradd --create-home --home-dir /home/user -g user user + +WORKDIR /home/user +RUN wget -q https://bitbucket.org/ariya/phantomjs/downloads/\ +phantomjs-2.1.1-linux-x86_64.tar.bz2 && \ tar xvjf phantomjs-2.1.1-linux-x86_64.tar.bz2 -C /usr/local/share/ && \ - ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs /usr/local/bin/ + ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs \ + /usr/local/bin/ # We need a few special packages for QN. -ENV R_LIBS="/usr/local/lib/R/site-library" +ENV R_LIBS=/usr/local/lib/R/site-library COPY common/R/install_devtools.R . RUN Rscript install_devtools.R @@ -83,22 +88,23 @@ COPY workers/R/dependencies/qn/bioc/dependencies.R . RUN Rscript dependencies.R # End QN-specific +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed numpy scipy matplotlib \ + pandas==0.25.3 scikit-learn sympy nose rpy2===3.4.5 tzlocal fancySVD + +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed -r requirements.txt && \ + # Pin setuptools as a workaround for + # https://github.com/pypa/setuptools/issues/3693 + pip3 install --ignore-installed setuptools==65.0.1 numpy==1.16.0 + # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ +RUN pip3 install --ignore-installed \ common/$(ls common -1 | sort --version-sort | tail -1) -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir numpy scipy matplotlib \ - pandas==0.25.3 scikit-learn sympy nose rpy2===3.4.5 tzlocal fancySVD - -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ - pip3 install --ignore-installed --no-cache-dir numpy==1.16.0 - ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 3a5974491..c65faf5dc 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -10,12 +10,16 @@ RUN Rscript install_downloader_R_only.R # Aspera will only install as the current user. # Even using `su - user &&` doesn't work... -USER user +USER user # Install Aspera. We have to install it using Holland Computing Center's conda # repo because download.asperasoft.com now returns 403s -RUN wget -q https://anaconda.org/HCC/aspera-cli/3.9.1/download/linux-64/aspera-cli-3.9.1-0.tar.bz2 && \ - [ "$(sha256sum aspera-cli-3.9.1-0.tar.bz2 | cut -d ' ' -f1)" = 60a09a7f3795186954079869106aa89a64183b7be8e0da7cbbe9d57c66c9bcdb ] && \ + +RUN wget -q "https://anaconda.org/HCC/aspera-cli/3.9.1/download/\ +linux-64/aspera-cli-3.9.1-0.tar.bz2" && \ + [ "$(sha256sum aspera-cli-3.9.1-0.tar.bz2 | cut -d ' ' -f1)" =\ + "60a09a7f3795186954079869106aa89a64183b7be8e0da7cbbe9d57c66c9bcdb" ] && \ + rm -rf .aspera && \ mkdir -p .aspera/cli && \ tar xf aspera-cli-3.9.1-0.tar.bz2 -C .aspera/cli && \ rm aspera-cli-3.9.1-0.tar.bz2 @@ -23,20 +27,21 @@ RUN wget -q https://anaconda.org/HCC/aspera-cli/3.9.1/download/linux-64/aspera-c # Now that we're done installing Aspera go back to being root for a bit. USER root -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir rpy2==3.4.5 +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed rpy2==3.4.5 # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ +RUN pip3 install --ignore-installed \ common/$(ls common -1 | sort --version-sort | tail -1) COPY workers/data_refinery_workers/downloaders/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip3 cache. + rm -r /root/.cache ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index 949c22405..fe65bf7ab 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -14,16 +14,18 @@ RUN Rscript dependencies.R # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ +RUN pip3 install --ignore-installed \ common/$(ls common -1 | sort --version-sort | tail -1) -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip +RUN pip3 install --ignore-installed --upgrade pip + COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt +RUN pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip3 cache. + rm -r /root/.cache ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index a0cb7855e..7f55589d0 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -14,29 +14,30 @@ RUN Rscript install_gene_convert.R # Noop-specific. RUN mkdir -p gene_indexes WORKDIR /home/user/gene_indexes -ENV ID_REFINERY_URL https://zenodo.org/record/1410647/files/all_1536267482.zip +ENV ID_REFINERY_URL=https://zenodo.org/record/1410647/files/all_1536267482.zip RUN curl -O $ID_REFINERY_URL && \ echo $ID_REFINERY_URL > /etc/identifier_refinery_url && \ unzip *.zip && \ rm *.zip # End Noop-specific. -WORKDIR /home/user - -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir numpy - -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt +WORKDIR /home/user/ # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ +RUN pip3 install --ignore-installed \ common/$(ls common -1 | sort --version-sort | tail -1) -ARG SYSTEM_VERSION +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed numpy + +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip3 cache. + rm -r /root/.cache -ENV SYSTEM_VERSION $SYSTEM_VERSION +ARG SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index 724ddac60..1bb8de6e6 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -10,11 +10,13 @@ WORKDIR /home/user # Salmon to work https://github.com/AlexsLemonade/refinebio/issues/1496. # This is something that should be considered when updating salmon, because # all samples from incomplete experiments must have salmon run on them again. -ENV SALMON_VERSION 0.13.1 +ENV SALMON_VERSION=0.13.1 -# On version 0.13.1 salmon was being extracted to a folder with an all lowercase name -# the options `-C` and `--strip-components` allow us to specify the name for the resulting file -RUN wget -q "https://github.com/COMBINE-lab/salmon/releases/download/v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ +# On version 0.13.1 salmon was being extracted to a folder with an all +# lowercase name the options `-C` and `--strip-components` allow us to specify +#the name for the resulting file. +RUN wget -q "https://github.com/COMBINE-lab/salmon/releases/download/\ +v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ mkdir "Salmon-${SALMON_VERSION}_linux_x86_64" && \ tar -xzf "Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" \ -C "Salmon-${SALMON_VERSION}_linux_x86_64" --strip-components 1 && \ @@ -30,16 +32,18 @@ RUN Rscript tximport_dependencies.R COPY workers/R/dependencies/tximport/install_tximport.R . RUN Rscript install_tximport.R -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir numpy +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed numpy # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ +RUN pip3 install --ignore-installed \ common/$(ls common -1 | sort --version-sort | tail -1) COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip3 cache. + rm -r /root/.cache # Install SalmonTools. RUN git clone https://github.com/COMBINE-lab/SalmonTools.git && \ @@ -50,14 +54,14 @@ RUN git clone https://github.com/COMBINE-lab/SalmonTools.git && \ rm -rf SalmonTools # Install sra-tools. -ENV SRA_VERSION 2.9.1 -RUN wget -q "https://ftp.ncbi.nlm.nih.gov/sra/sdk/${SRA_VERSION}/sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ +ENV SRA_VERSION=2.9.1 +RUN wget -q "https://ftp.ncbi.nlm.nih.gov/sra/sdk/${SRA_VERSION}/\ +sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ tar zxfv "sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ cp -r "sratoolkit.${SRA_VERSION}-ubuntu64/bin/"* /usr/bin ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index 00ea02baa..ebdf924a4 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -1,30 +1,31 @@ -FROM ccdlstaging/dr_worker_base:latest +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -RUN pip3 install --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir nose numpy rpy2==3.4.5 +COPY workers/R/dependencies/qn/cran/dependencies.R . +RUN Rscript dependencies.R + +COPY workers/R/dependencies/qn/bioc/dependencies.R . +RUN Rscript dependencies.R + +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed nose numpy rpy2==3.4.5 # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ +RUN pip3 install --ignore-installed \ common/$(ls common -1 | sort --version-sort | tail -1) COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt - -COPY workers/R/dependencies/qn/cran/dependencies.R . -RUN Rscript dependencies.R - -COPY workers/R/dependencies/qn/bioc/dependencies.R . -RUN Rscript dependencies.R +RUN pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip3 cache. + rm -rf /root/.cache ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index 1c04e7390..8ce0d974a 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -1,4 +1,4 @@ -FROM ccdlstaging/dr_worker_base:latest +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] @@ -15,10 +15,11 @@ RUN git clone https://github.com/deweylab/RSEM.git && \ # Salmon to work https://github.com/AlexsLemonade/refinebio/issues/1496. # This is something that should be considered when updating salmon, because # all samples from incomplete experiments must have salmon run on them again. -ENV SALMON_VERSION 0.13.1 +ENV SALMON_VERSION=0.13.1 # Salmon can extract to a different directory than the name of the tar file. -RUN wget -q "https://github.com/COMBINE-lab/salmon/releases/download/v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ +RUN wget -q "https://github.com/COMBINE-lab/salmon/releases/download/\ +v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ tar -xzf "Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ cp "$(tar -tzf Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz | \ head -1 | cut -f1 -d '/')/bin/salmon" /usr/local/bin && \ @@ -27,19 +28,20 @@ RUN wget -q "https://github.com/COMBINE-lab/salmon/releases/download/v${SALMON_V rm -r Salmon* # End Salmon installation. -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir numpy +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed numpy COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ +RUN pip3 install --ignore-installed \ common/$(ls common -1 | sort --version-sort | tail -1) COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip3 cache. + rm -rf /root/.cache ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.worker_base b/workers/dockerfiles/Dockerfile.worker_base deleted file mode 100644 index d8f1a0588..000000000 --- a/workers/dockerfiles/Dockerfile.worker_base +++ /dev/null @@ -1,68 +0,0 @@ -FROM ubuntu:18.04 - -# Fail in case of an error at any stage in the pipe. -SHELL ["/bin/bash", "-o", "pipefail", "-c"] - -WORKDIR /home/user - -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG=C.UTF-8 - -RUN apt-get update && \ - apt-get install --no-install-recommends -y software-properties-common && \ - add-apt-repository ppa:apt-fast/stable && \ - add-apt-repository ppa:deadsnakes/ppa && \ - add-apt-repository ppa:savoury1/llvm-defaults-10 && \ - apt-get update -qq && \ - apt-get install --no-install-recommends -y apt-fast apt-transport-https gpg-agent - -# The packages related to R are somewhat weird, see the README for more details. -COPY workers/CRAN.gpg . -RUN apt-key add CRAN.gpg && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list - -RUN apt-fast update -qq && apt-fast install -y \ - build-essential \ - ccache \ - cmake \ - curl \ - cython3 \ - ed \ - git \ - libcairo-dev \ - libcurl4-gnutls-dev \ - libedit-dev \ - libgit2-dev \ - libpq-dev \ - libssl-dev \ - libxml2-dev \ - llvm-10-dev \ - lsb-release \ - mercurial \ - pkg-config \ - python3-pip \ - python3.8 \ - python3.8-dev \ - r-base-core \ - wget && \ - rm CRAN.gpg && \ - apt-get clean && \ - rm -rf /var/lib/apt/lists/* && \ - ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ - update-alternatives --install /usr/bin/python3 python3 /usr/bin/python3.8 1 && \ - groupadd user && useradd --create-home --home-dir /home/user -g user user - -# Set up ccache. -COPY workers/ccache.conf /root/.ccache/ccache.conf -RUN for i in c++ cc g++ gcc; do ln -s /usr/bin/ccache /usr/local/bin/$i; done -ENV PATH="/usr/local/bin:${PATH}" - -# Pre-install dev tools. -ENV R_LIBS="/usr/local/lib/R/site-library" -COPY common/R/install_devtools.R . -RUN Rscript install_devtools.R From 13089441a9f96b8778fcb8852e62be26eb017b6d Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 28 Nov 2022 10:42:12 -0800 Subject: [PATCH 051/220] Add another docker images update. --- workers/dockerfiles/Dockerfile.compendia | 8 +++----- workers/dockerfiles/Dockerfile.downloaders | 2 +- workers/dockerfiles/Dockerfile.illumina | 2 +- workers/dockerfiles/Dockerfile.no_op | 2 +- workers/dockerfiles/Dockerfile.salmon | 2 +- 5 files changed, 7 insertions(+), 9 deletions(-) diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index f2afa185f..4dd80559f 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -69,8 +69,8 @@ RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -RUN wget -q https://bitbucket.org/ariya/phantomjs/downloads/\ -phantomjs-2.1.1-linux-x86_64.tar.bz2 && \ +RUN wget "https://bitbucket.org/ariya/phantomjs/downloads/\ +phantomjs-2.1.1-linux-x86_64.tar.bz2" && \ tar xvjf phantomjs-2.1.1-linux-x86_64.tar.bz2 -C /usr/local/share/ && \ ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs \ /usr/local/bin/ @@ -94,9 +94,7 @@ RUN pip3 install --ignore-installed --upgrade pip && \ COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed -r requirements.txt && \ - # Pin setuptools as a workaround for - # https://github.com/pypa/setuptools/issues/3693 - pip3 install --ignore-installed setuptools==65.0.1 numpy==1.16.0 + pip3 install --ignore-installed numpy==1.16.0 # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index c65faf5dc..145d567e1 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -1,4 +1,4 @@ -FROM ccdlstaging/dr_worker_base:latest +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index fe65bf7ab..648d2e758 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -1,4 +1,4 @@ -FROM ccdlstaging/dr_worker_base:latest +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index 7f55589d0..8d55281ca 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -1,4 +1,4 @@ -FROM ccdlstaging/dr_worker_base:latest +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index 1bb8de6e6..b6395ef58 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -1,4 +1,4 @@ -FROM ccdlstaging/dr_worker_base:latest +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] From 5e79a6ee007b46628d55f26b4a6ed18641cccb6c Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 28 Nov 2022 15:24:59 -0800 Subject: [PATCH 052/220] Refactor API docker files. --- api/dockerfiles/Dockerfile.api_local | 26 +++++++++------- api/dockerfiles/Dockerfile.api_production | 36 +++++++++++++---------- 2 files changed, 35 insertions(+), 27 deletions(-) diff --git a/api/dockerfiles/Dockerfile.api_local b/api/dockerfiles/Dockerfile.api_local index 05dd00df1..c333aa29a 100644 --- a/api/dockerfiles/Dockerfile.api_local +++ b/api/dockerfiles/Dockerfile.api_local @@ -1,29 +1,33 @@ FROM python:3.8.5-slim-buster +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] + # Install gcc and libpq-dev for psycopg2. -RUN apt-get update -qq -RUN apt-get install -y \ - gcc \ - libpq-dev +RUN apt-get update -qq && \ + apt-get install -y \ + gcc \ + libpq-dev -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user +RUN groupadd user && \ + useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -RUN pip install --upgrade pip - COPY api/requirements.txt . -RUN pip install -r requirements.txt +RUN pip install --upgrade pip && \ + pip install --ignore-installed -r requirements.txt && \ + rm -r /root/.cache # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip install common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip install --ignore-installed \ + common/$(ls common -1 | sort --version-sort | tail -1) COPY api/ . COPY config/ config/ ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/api/dockerfiles/Dockerfile.api_production b/api/dockerfiles/Dockerfile.api_production index a432610ff..37184be03 100644 --- a/api/dockerfiles/Dockerfile.api_production +++ b/api/dockerfiles/Dockerfile.api_production @@ -1,34 +1,38 @@ FROM python:3.8.5-buster +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] + # Install gcc and libpq-dev for psycopg2. -RUN apt-get update -qq -RUN apt-get install -y \ - gcc \ - libpq-dev +RUN apt-get update -qq && \ + apt-get install -y \ + gcc \ + libpq-dev -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user +RUN groupadd user && \ + useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user COPY api/requirements.txt . -RUN pip install -r requirements.txt +RUN pip install --upgrade pip && \ + pip install --ignore-installed -r requirements.txt && \ + pip install --ignore-installed uwsgi && \ + rm -r /root/.cache # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip install common/$(ls common -1 | sort --version-sort | tail -1) - -# uWSGI. -RUN pip install uwsgi +RUN pip install --ignore-installed \ + common/$(ls common -1 | sort --version-sort | tail -1) COPY api/ . COPY config/ config/ -RUN chmod +x /home/user/collect_and_run_uwsgi.sh -RUN mkdir -p /tmp/www/static -RUN chown user /tmp/www/static +RUN chmod +x /home/user/collect_and_run_uwsgi.sh && \ + mkdir -p /tmp/www/static && \ + chown user /tmp/www/static ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user @@ -38,4 +42,4 @@ VOLUME /tmp/www/static EXPOSE 8081 -CMD ["/home/user/collect_and_run_uwsgi.sh"] +CMD ["sh", "-c", "/home/user/collect_and_run_uwsgi.sh"] From d72d10cd8bb4df068357647d7d5cf468d6d1b8df Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 28 Nov 2022 16:31:37 -0800 Subject: [PATCH 053/220] Update foreman docker image. --- foreman/dockerfiles/Dockerfile.foreman | 13 +++++++------ 1 file changed, 7 insertions(+), 6 deletions(-) diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index f95cb393e..8fad92fd9 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -3,8 +3,6 @@ FROM ubuntu:18.04 # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] -WORKDIR /home/user - # Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 # For whatever reason this worked and 'en_US.UTF-8' did not. ENV LANG=C.UTF-8 @@ -24,25 +22,28 @@ RUN apt-get update -qq && \ python3 \ python3-pip && \ apt-get clean && \ + rm -rf /var/lib/apt/lists/* && \ groupadd user && \ useradd --create-home --home-dir /home/user -g user user && \ pip3 install --ignore-installed --no-cache-dir --upgrade pip +WORKDIR /home/user + # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ +RUN pip3 install --ignore-installed \ common/$(ls common -1 | sort --version-sort | tail -1) COPY foreman/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN pip3 install --ignore-installed -r requirements.txt && \ + rm -rf /root/.cache COPY .boto .boto COPY config config COPY foreman/ . ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user From 5fd3b1e90b5d5edf7361eae1f291ee0744b8df9d Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 28 Nov 2022 18:17:38 -0800 Subject: [PATCH 054/220] Update common docker images. Reorder and clean up the instructions. --- common/dockerfiles/Dockerfile.common_tests | 75 +++-------- common/dockerfiles/Dockerfile.migrations | 21 ++-- common/requirements.in | 2 + common/requirements.txt | 140 +++++++++++++++------ 4 files changed, 134 insertions(+), 104 deletions(-) diff --git a/common/dockerfiles/Dockerfile.common_tests b/common/dockerfiles/Dockerfile.common_tests index da18704b2..c47468be6 100644 --- a/common/dockerfiles/Dockerfile.common_tests +++ b/common/dockerfiles/Dockerfile.common_tests @@ -1,79 +1,36 @@ -FROM ubuntu:18.04 +FROM ccdlstaging/dr_base:latest -RUN apt-get update -qq -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN apt-get update -qq -RUN apt-get -y install apt-fast +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - -# The packages related to R are somewhat weird, see the README for more details. - -COPY workers/CRAN.gpg . -RUN \ - apt-fast update -qq && \ - apt-get install -y apt-transport-https && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq && \ - apt-fast install -y \ - ed \ - git \ - python3 \ - python3-pip \ - python3-dev \ - r-base-core \ - r-base-dev \ - libpq-dev \ - libxml2-dev \ - libssl-dev \ - libcurl4-openssl-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* -RUN rm CRAN.gpg +# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 +# For whatever reason this worked and 'en_US.UTF-8' did not. +ENV LANG=C.UTF-8 -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -ENV R_LIBS "/usr/local/lib/R/site-library" - -COPY common/install_devtools.R . - -RUN Rscript install_devtools.R - -COPY workers/install_bioc.R . +ENV R_LIBS=/usr/local/lib/R/site-library +COPY workers/R/dependencies/install_bioc.R . RUN Rscript install_bioc.R -COPY workers/install_affy_only.R . - +COPY workers/R/dependencies/affymetrix/install_affy_only.R . RUN Rscript install_affy_only.R -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - COPY config config COPY common/requirements.txt . - -RUN pip3 install --no-cache-dir -r requirements.txt - -# Install this rpy2 here instead of via requirements.txt because -# pip-compile throws an error for it. -RUN pip3 install rpy2==3.4.5 +RUN pip3 install --ignore-installed -r requirements.txt && \ + # Install this rpy2 here instead of via requirements.txt because + # pip-compile throws an error for it. + pip3 install rpy2==3.4.5 && \ + rm -rf /root/.cache COPY common/ . ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -ENTRYPOINT [""] +ENTRYPOINT [] diff --git a/common/dockerfiles/Dockerfile.migrations b/common/dockerfiles/Dockerfile.migrations index 7c042f255..e3f8c6fcb 100644 --- a/common/dockerfiles/Dockerfile.migrations +++ b/common/dockerfiles/Dockerfile.migrations @@ -1,21 +1,24 @@ FROM python:3.8.5-slim-buster -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user -WORKDIR /home/user +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Needed for migration 0006 -COPY config/ config +RUN groupadd user && \ + useradd --create-home --home-dir /home/user -g user user -COPY common/requirements.txt . +WORKDIR /home/user -RUN pip install -r requirements.txt +COPY common/requirements.txt . +RUN pip install --ignore-installed -r requirements.txt && \ + rm -rf /root/.cache COPY common/ . +# Needed for migration 0006. +COPY config config ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -ENTRYPOINT [""] +ENTRYPOINT [] diff --git a/common/requirements.in b/common/requirements.in index a5d3e724a..54ef9542f 100644 --- a/common/requirements.in +++ b/common/requirements.in @@ -16,3 +16,5 @@ vcrpy django-computedfields>=0.1.5 pyyaml>=5.4 sqlparse>=0.4.2 +markupsafe==2.0.1 + # via jinja2 # via boto3, botocore diff --git a/common/requirements.txt b/common/requirements.txt index 088feeb37..a5f2f91a0 100644 --- a/common/requirements.txt +++ b/common/requirements.txt @@ -1,40 +1,108 @@ # -# This file is autogenerated by pip-compile +# This file is autogenerated by pip-compile with python 3.9 # To update, run: # -# pip-compile requirements.in +# pip-compile common/requirements.in # -asgiref==3.3.4 # via django -boto3==1.17.93 # via -r requirements.in -botocore==1.20.93 # via boto3, s3transfer -certifi==2021.5.30 # via requests -chardet==4.0.0 # via requests -coverage==5.5 # via -r requirements.in -daiquiri==3.0.0 # via -r requirements.in -django-computedfields==0.1.5 # via -r requirements.in -django-elasticsearch-dsl-drf==0.22.1 # via -r requirements.in -django-elasticsearch-dsl==6.5.0 # via -r requirements.in, django-elasticsearch-dsl-drf -django-nine==0.2.4 # via django-elasticsearch-dsl-drf -django==3.2.7 # via -r requirements.in, django-nine, djangorestframework -djangorestframework==3.12.4 # via -r requirements.in, django-elasticsearch-dsl-drf -elasticsearch-dsl==6.4.0 # via -r requirements.in, django-elasticsearch-dsl, django-elasticsearch-dsl-drf -elasticsearch==6.8.2 # via django-elasticsearch-dsl-drf, elasticsearch-dsl -idna==2.10 # via requests, yarl -jmespath==0.10.0 # via boto3, botocore -multidict==5.1.0 # via yarl -psycopg2-binary==2.8.6 # via -r requirements.in -python-dateutil==2.8.1 # via botocore, elasticsearch-dsl -python-json-logger==2.0.1 # via daiquiri -pytz==2021.1 # via django -pyyaml==5.4.1 # via -r requirements.in, vcrpy -raven==6.10.0 # via -r requirements.in -requests==2.25.1 # via -r requirements.in -retrying==1.3.3 # via -r requirements.in -s3transfer==0.4.2 # via boto3 -six==1.16.0 # via django-elasticsearch-dsl, django-elasticsearch-dsl-drf, elasticsearch-dsl, python-dateutil, retrying, vcrpy -sqlparse==0.4.2 # via -r requirements.in, django -typing-extensions==3.10.0.0 # via asgiref, yarl -urllib3==1.26.5 # via -r requirements.in, botocore, elasticsearch, requests -vcrpy==4.1.1 # via -r requirements.in -wrapt==1.12.1 # via vcrpy -yarl==1.6.3 # via vcrpy +asgiref==3.3.4 + # via django +boto3==1.17.93 + # via -r common/requirements.in +botocore==1.20.93 + # via + # boto3 + # s3transfer +certifi==2021.5.30 + # via requests +chardet==4.0.0 + # via requests +coverage==5.5 + # via -r common/requirements.in +daiquiri==3.0.0 + # via -r common/requirements.in +django==3.2.7 + # via + # -r common/requirements.in + # django-nine + # djangorestframework +django-computedfields==0.1.5 + # via -r common/requirements.in +django-elasticsearch-dsl==6.5.0 + # via + # -r common/requirements.in + # django-elasticsearch-dsl-drf +django-elasticsearch-dsl-drf==0.22.1 + # via -r common/requirements.in +django-nine==0.2.4 + # via django-elasticsearch-dsl-drf +djangorestframework==3.12.4 + # via + # -r common/requirements.in + # django-elasticsearch-dsl-drf +elasticsearch==6.8.2 + # via + # django-elasticsearch-dsl-drf + # elasticsearch-dsl +elasticsearch-dsl==6.4.0 + # via + # -r common/requirements.in + # django-elasticsearch-dsl + # django-elasticsearch-dsl-drf +idna==2.10 + # via + # requests + # yarl +jmespath==0.10.0 + # via + # boto3 + # botocore +markupsafe==2.0.1 + # via -r common/requirements.in +multidict==5.1.0 + # via yarl +psycopg2-binary==2.8.6 + # via -r common/requirements.in +python-dateutil==2.8.1 + # via + # botocore + # elasticsearch-dsl +python-json-logger==2.0.1 + # via daiquiri +pytz==2021.1 + # via django +pyyaml==5.4.1 + # via + # -r common/requirements.in + # vcrpy +raven==6.10.0 + # via -r common/requirements.in +requests==2.25.1 + # via -r common/requirements.in +retrying==1.3.3 + # via -r common/requirements.in +s3transfer==0.4.2 + # via boto3 +six==1.16.0 + # via + # django-elasticsearch-dsl + # django-elasticsearch-dsl-drf + # elasticsearch-dsl + # python-dateutil + # retrying + # vcrpy +sqlparse==0.4.2 + # via + # -r common/requirements.in + # django +urllib3==1.26.5 + # via + # -r common/requirements.in + # botocore + # elasticsearch + # requests +vcrpy==4.1.1 + # via -r common/requirements.in +wrapt==1.12.1 + # via vcrpy +yarl==1.6.3 + # via vcrpy From f7191badbd0d28d8ef944bd91e5287d8ba3a918b Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 1 Dec 2022 17:05:49 -0800 Subject: [PATCH 055/220] Optimize docker images, put cache invalidating instructions to the tail. --- .pre-commit-config.yaml | 2 +- .../dockerfiles/Dockerfile.affymetrix_local | 10 +-- workers/dockerfiles/Dockerfile.compendia | 36 +++++------ workers/dockerfiles/Dockerfile.downloaders | 34 +++++------ workers/dockerfiles/Dockerfile.illumina | 25 ++++---- workers/dockerfiles/Dockerfile.no_op | 35 +++++------ workers/dockerfiles/Dockerfile.salmon | 61 +++++++++---------- workers/dockerfiles/Dockerfile.smasher | 19 +++--- workers/dockerfiles/Dockerfile.transcriptome | 29 +++++---- 9 files changed, 124 insertions(+), 127 deletions(-) diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index d024704da..b651ce24a 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -22,7 +22,7 @@ repos: - id: isort - repo: https://github.com/psf/black - rev: 19.10b0 + rev: 22.3.0 hooks: - id: black args: [--line-length=100] diff --git a/workers/dockerfiles/Dockerfile.affymetrix_local b/workers/dockerfiles/Dockerfile.affymetrix_local index 7f1af8ad6..08db9e197 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix_local +++ b/workers/dockerfiles/Dockerfile.affymetrix_local @@ -6,20 +6,20 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user # Remove the version of common already installed. -RUN rm -r common && \ +RUN rm -rf common && \ pip3 uninstall -y data_refinery_common # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ RUN pip3 install --ignore-installed --no-cache-dir \ - common/$(ls common -1 | sort --version-sort | tail -1) + common/$(ls common -1 | sort --version-sort | tail -1) + +COPY config/ config/ +COPY workers/ . ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -COPY config/ config/ -COPY workers/ . - ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 4dd80559f..9a44a7ecc 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -69,11 +69,6 @@ RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -RUN wget "https://bitbucket.org/ariya/phantomjs/downloads/\ -phantomjs-2.1.1-linux-x86_64.tar.bz2" && \ - tar xvjf phantomjs-2.1.1-linux-x86_64.tar.bz2 -C /usr/local/share/ && \ - ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs \ - /usr/local/bin/ # We need a few special packages for QN. ENV R_LIBS=/usr/local/lib/R/site-library @@ -86,28 +81,35 @@ RUN Rscript dependencies.R COPY workers/R/dependencies/qn/bioc/dependencies.R . RUN Rscript dependencies.R -# End QN-specific - -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed numpy scipy matplotlib \ - pandas==0.25.3 scikit-learn sympy nose rpy2===3.4.5 tzlocal fancySVD +# End QN-specific. COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - pip3 install --ignore-installed numpy==1.16.0 +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed nose numpy scipy matplotlib \ + pandas==0.25.3 scikit-learn sympy rpy2===3.4.5 tzlocal fancySVD && \ + pip3 install --ignore-installed -r requirements.txt && \ + pip3 install --ignore-installed numpy==1.16.0 && \ + # Clear out the pip cache. + rm -rf /root/.cache # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) + common/$(ls common -1 | sort --version-sort | tail -1) && \ + # Install phantomjs. + wget "https://bitbucket.org/ariya/phantomjs/downloads/\ +phantomjs-2.1.1-linux-x86_64.tar.bz2" && \ + tar xvjf phantomjs-2.1.1-linux-x86_64.tar.bz2 -C /usr/local/share/ && \ + ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs \ + /usr/local/bin/ + +COPY .boto .boto +COPY config/ config/ +COPY workers/ . ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -COPY .boto .boto -COPY config/ config/ -COPY workers/ . - ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 145d567e1..02917518d 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -8,13 +8,24 @@ WORKDIR /home/user COPY workers/R/dependencies/install_downloader_R_only.R . RUN Rscript install_downloader_R_only.R +COPY workers/data_refinery_workers/downloaders/requirements.txt . +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed rpy2==3.4.5 && \ + pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip cache. + rm -rf /root/.cache + +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed \ + common/$(ls common -1 | sort --version-sort | tail -1) + # Aspera will only install as the current user. # Even using `su - user &&` doesn't work... - USER user + # Install Aspera. We have to install it using Holland Computing Center's conda # repo because download.asperasoft.com now returns 403s - RUN wget -q "https://anaconda.org/HCC/aspera-cli/3.9.1/download/\ linux-64/aspera-cli-3.9.1-0.tar.bz2" && \ [ "$(sha256sum aspera-cli-3.9.1-0.tar.bz2 | cut -d ' ' -f1)" =\ @@ -27,26 +38,13 @@ linux-64/aspera-cli-3.9.1-0.tar.bz2" && \ # Now that we're done installing Aspera go back to being root for a bit. USER root -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed rpy2==3.4.5 - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) - -COPY workers/data_refinery_workers/downloaders/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip3 cache. - rm -r /root/.cache +COPY .boto .boto +COPY config config +COPY workers/ . ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -COPY .boto .boto -COPY config config -COPY workers/ . - ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index 648d2e758..e578b0b69 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -12,22 +12,16 @@ RUN Rscript dependencies.R COPY workers/R/dependencies/illumina/bioc/dependencies.R . RUN Rscript dependencies.R +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip cache. + rm -rf /root/.cache + # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) - -RUN pip3 install --ignore-installed --upgrade pip - -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip3 cache. - rm -r /root/.cache - -ARG SYSTEM_VERSION -ENV SYSTEM_VERSION=$SYSTEM_VERSION - -USER user + common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto COPY config/ config/ @@ -35,4 +29,9 @@ COPY workers/ . COPY workers/data_refinery_workers/processors/detect_database.R . COPY workers/illumina_probe_maps/ probe_maps/ +ARG SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION + +USER user + ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index 8d55281ca..0d98229ee 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -11,9 +11,23 @@ RUN Rscript dependencies.R COPY workers/R/dependencies/no_op/install_gene_convert.R . RUN Rscript install_gene_convert.R +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed numpy && \ + pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip cache. + rm -rf /root/.cache + +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed \ + common/$(ls common -1 | sort --version-sort | tail -1) + # Noop-specific. RUN mkdir -p gene_indexes + WORKDIR /home/user/gene_indexes + ENV ID_REFINERY_URL=https://zenodo.org/record/1410647/files/all_1536267482.zip RUN curl -O $ID_REFINERY_URL && \ echo $ID_REFINERY_URL > /etc/identifier_refinery_url && \ @@ -21,28 +35,15 @@ RUN curl -O $ID_REFINERY_URL && \ rm *.zip # End Noop-specific. -WORKDIR /home/user/ - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) - -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed numpy +WORKDIR /home/user -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip3 cache. - rm -r /root/.cache +COPY .boto .boto +COPY config/ config/ +COPY workers/ . ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -COPY .boto .boto -COPY config/ config/ -COPY workers/ . - ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index b6395ef58..b4493b2e9 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -5,6 +5,25 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user +COPY workers/R/dependencies/tximport/cran/dependencies.R tximport_dependencies.R +RUN Rscript tximport_dependencies.R + +# Install tximport. +COPY workers/R/dependencies/tximport/install_tximport.R . +RUN Rscript install_tximport.R + +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed numpy && \ + pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip cache. + rm -rf /root/.cache + +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed \ + common/$(ls common -1 | sort --version-sort | tail -1) + # Install Salmon # Tximport requires all experiments to be processed with the same version of # Salmon to work https://github.com/AlexsLemonade/refinebio/issues/1496. @@ -25,48 +44,28 @@ v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ rm -f "Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" # End Salmon installation. -COPY workers/R/dependencies/tximport/cran/dependencies.R tximport_dependencies.R -RUN Rscript tximport_dependencies.R - -# Install tximport. -COPY workers/R/dependencies/tximport/install_tximport.R . -RUN Rscript install_tximport.R - -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed numpy - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) - -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip3 cache. - rm -r /root/.cache - -# Install SalmonTools. -RUN git clone https://github.com/COMBINE-lab/SalmonTools.git && \ +ENV SRA_VERSION=2.9.1 +RUN \ + # Install SalmonTools. + git clone https://github.com/COMBINE-lab/SalmonTools.git && \ cd SalmonTools && \ git checkout 3e6654c2c10a5225498b623056993947fa688afc && \ cmake . -DCMAKE_INSTALL_PREFIX=/usr/local && \ make install && \ - rm -rf SalmonTools - -# Install sra-tools. -ENV SRA_VERSION=2.9.1 -RUN wget -q "https://ftp.ncbi.nlm.nih.gov/sra/sdk/${SRA_VERSION}/\ + rm -rf SalmonTools && \ + # Install sra-tools. + wget -q "https://ftp.ncbi.nlm.nih.gov/sra/sdk/${SRA_VERSION}/\ sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ tar zxfv "sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ cp -r "sratoolkit.${SRA_VERSION}-ubuntu64/bin/"* /usr/bin +COPY .boto .boto +COPY config/ config/ +COPY workers/ . + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -COPY .boto .boto -COPY config/ config/ -COPY workers/ . - ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index ebdf924a4..bdf146474 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -11,26 +11,25 @@ RUN Rscript dependencies.R COPY workers/R/dependencies/qn/bioc/dependencies.R . RUN Rscript dependencies.R +COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed nose numpy rpy2==3.4.5 + pip3 install --ignore-installed nose numpy rpy2==3.4.5 && \ + pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip cache. + rm -rf /root/.cache # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) + common/$(ls common -1 | sort --version-sort | tail -1) -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip3 cache. - rm -rf /root/.cache +COPY .boto .boto +COPY config/ config/ +COPY workers/ . ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -COPY .boto .boto -COPY config/ config/ -COPY workers/ . - ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index 8ce0d974a..ae75c87a8 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -5,6 +5,17 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --upgrade pip && \ + pip3 install --ignore-installed numpy && \ + pip3 install --ignore-installed -r requirements.txt && \ + # Clear out the pip cache. + rm -rf /root/.cache + +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed \ + common/$(ls common -1 | sort --version-sort | tail -1) + # It's annoying that this can only be installed via git. RUN git clone https://github.com/deweylab/RSEM.git && \ cd RSEM && make install && \ @@ -28,25 +39,13 @@ v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ rm -r Salmon* # End Salmon installation. -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed numpy - -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) - -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip3 cache. - rm -rf /root/.cache +COPY .boto .boto +COPY config/ config/ +COPY workers/ . ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -COPY .boto .boto -COPY config/ config/ -COPY workers/ . - ENTRYPOINT [] From 8b8de4e0d600576be19aa0475d47cfea07e7cfd1 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 2 Dec 2022 10:51:06 -0800 Subject: [PATCH 056/220] Add `--no-cache-dir`, optimize order. --- common/dockerfiles/Dockerfile.common_tests | 11 ++++------- common/dockerfiles/Dockerfile.migrations | 3 +-- 2 files changed, 5 insertions(+), 9 deletions(-) diff --git a/common/dockerfiles/Dockerfile.common_tests b/common/dockerfiles/Dockerfile.common_tests index c47468be6..d795603d7 100644 --- a/common/dockerfiles/Dockerfile.common_tests +++ b/common/dockerfiles/Dockerfile.common_tests @@ -6,27 +6,24 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] # Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 # For whatever reason this worked and 'en_US.UTF-8' did not. ENV LANG=C.UTF-8 +ENV R_LIBS=/usr/local/lib/R/site-library WORKDIR /home/user -ENV R_LIBS=/usr/local/lib/R/site-library - COPY workers/R/dependencies/install_bioc.R . RUN Rscript install_bioc.R COPY workers/R/dependencies/affymetrix/install_affy_only.R . RUN Rscript install_affy_only.R -COPY config config - COPY common/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ # Install this rpy2 here instead of via requirements.txt because # pip-compile throws an error for it. - pip3 install rpy2==3.4.5 && \ - rm -rf /root/.cache + pip3 install --ignore-installed --no-cache-dir rpy2==3.4.5 COPY common/ . +COPY config config ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/common/dockerfiles/Dockerfile.migrations b/common/dockerfiles/Dockerfile.migrations index e3f8c6fcb..f91316128 100644 --- a/common/dockerfiles/Dockerfile.migrations +++ b/common/dockerfiles/Dockerfile.migrations @@ -9,8 +9,7 @@ RUN groupadd user && \ WORKDIR /home/user COPY common/requirements.txt . -RUN pip install --ignore-installed -r requirements.txt && \ - rm -rf /root/.cache +RUN pip install --ignore-installed --no-cache-dir -r requirements.txt COPY common/ . # Needed for migration 0006. From 28209606cbbba184875693f0ba637c9b1d4432f3 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 2 Dec 2022 10:55:05 -0800 Subject: [PATCH 057/220] Add `--no-cache-dir`. --- foreman/dockerfiles/Dockerfile.foreman | 13 ++++++------- 1 file changed, 6 insertions(+), 7 deletions(-) diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 8fad92fd9..043ab3700 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -25,18 +25,17 @@ RUN apt-get update -qq && \ rm -rf /var/lib/apt/lists/* && \ groupadd user && \ useradd --create-home --home-dir /home/user -g user user && \ - pip3 install --ignore-installed --no-cache-dir --upgrade pip + pip3 install --ignore-installed --upgrade pip WORKDIR /home/user +COPY foreman/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt + # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) - -COPY foreman/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - rm -rf /root/.cache +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto COPY config config From a10865346ddce740acdbc1d6f6bb9513c94f03f5 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 2 Dec 2022 17:04:51 -0800 Subject: [PATCH 058/220] Add `--no-cache-dir` to pip. Move common package intallation to the bottom. --- workers/dockerfiles/Dockerfile.compendia | 23 +++++++++---------- workers/dockerfiles/Dockerfile.downloaders | 18 +++++++-------- workers/dockerfiles/Dockerfile.illumina | 8 +++---- workers/dockerfiles/Dockerfile.no_op | 18 +++++++-------- workers/dockerfiles/Dockerfile.salmon | 24 +++++++++----------- workers/dockerfiles/Dockerfile.smasher | 10 ++++---- workers/dockerfiles/Dockerfile.transcriptome | 21 ++++++++--------- 7 files changed, 54 insertions(+), 68 deletions(-) diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 9a44a7ecc..8790d859c 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -84,25 +84,24 @@ RUN Rscript dependencies.R # End QN-specific. COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed nose numpy scipy matplotlib \ +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir nose numpy scipy matplotlib \ pandas==0.25.3 scikit-learn sympy rpy2===3.4.5 tzlocal fancySVD && \ - pip3 install --ignore-installed -r requirements.txt && \ - pip3 install --ignore-installed numpy==1.16.0 && \ - # Clear out the pip cache. - rm -rf /root/.cache + pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ + pip3 install --ignore-installed --no-cache-dir numpy==1.16.0 -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) && \ - # Install phantomjs. - wget "https://bitbucket.org/ariya/phantomjs/downloads/\ +# Install phantomjs. +RUN wget -q "https://bitbucket.org/ariya/phantomjs/downloads/\ phantomjs-2.1.1-linux-x86_64.tar.bz2" && \ tar xvjf phantomjs-2.1.1-linux-x86_64.tar.bz2 -C /usr/local/share/ && \ ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs \ /usr/local/bin/ +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) + COPY .boto .boto COPY config/ config/ COPY workers/ . diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 02917518d..9b611956d 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -9,16 +9,9 @@ COPY workers/R/dependencies/install_downloader_R_only.R . RUN Rscript install_downloader_R_only.R COPY workers/data_refinery_workers/downloaders/requirements.txt . -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed rpy2==3.4.5 && \ - pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip cache. - rm -rf /root/.cache - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir rpy2==3.4.5 && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Aspera will only install as the current user. # Even using `su - user &&` doesn't work... @@ -38,6 +31,11 @@ linux-64/aspera-cli-3.9.1-0.tar.bz2" && \ # Now that we're done installing Aspera go back to being root for a bit. USER root +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) + COPY .boto .boto COPY config config COPY workers/ . diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index e578b0b69..200b37725 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -13,14 +13,12 @@ COPY workers/R/dependencies/illumina/bioc/dependencies.R . RUN Rscript dependencies.R COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip cache. - rm -rf /root/.cache +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ +RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index 0d98229ee..60f686265 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -12,16 +12,9 @@ COPY workers/R/dependencies/no_op/install_gene_convert.R . RUN Rscript install_gene_convert.R COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed numpy && \ - pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip cache. - rm -rf /root/.cache - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir numpy && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Noop-specific. RUN mkdir -p gene_indexes @@ -37,6 +30,11 @@ RUN curl -O $ID_REFINERY_URL && \ WORKDIR /home/user +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) + COPY .boto .boto COPY config/ config/ COPY workers/ . diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index b4493b2e9..046ee0b89 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -13,16 +13,9 @@ COPY workers/R/dependencies/tximport/install_tximport.R . RUN Rscript install_tximport.R COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed numpy && \ - pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip cache. - rm -rf /root/.cache - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir numpy && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Install Salmon # Tximport requires all experiments to be processed with the same version of @@ -45,9 +38,9 @@ v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ # End Salmon installation. ENV SRA_VERSION=2.9.1 -RUN \ - # Install SalmonTools. - git clone https://github.com/COMBINE-lab/SalmonTools.git && \ + +# Install SalmonTools. +RUN git clone https://github.com/COMBINE-lab/SalmonTools.git && \ cd SalmonTools && \ git checkout 3e6654c2c10a5225498b623056993947fa688afc && \ cmake . -DCMAKE_INSTALL_PREFIX=/usr/local && \ @@ -59,6 +52,11 @@ sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ tar zxfv "sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ cp -r "sratoolkit.${SRA_VERSION}-ubuntu64/bin/"* /usr/bin +# Get the latest version from the dist directory. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) + COPY .boto .boto COPY config/ config/ COPY workers/ . diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index bdf146474..926f98208 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -12,15 +12,13 @@ COPY workers/R/dependencies/qn/bioc/dependencies.R . RUN Rscript dependencies.R COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed nose numpy rpy2==3.4.5 && \ - pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip cache. - rm -rf /root/.cache +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir nose numpy rpy2==3.4.5 && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ +RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index ae75c87a8..f7dbe3676 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -6,18 +6,11 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --upgrade pip && \ - pip3 install --ignore-installed numpy && \ - pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip cache. - rm -rf /root/.cache - -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) - -# It's annoying that this can only be installed via git. -RUN git clone https://github.com/deweylab/RSEM.git && \ +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ + pip3 install --ignore-installed --no-cache-dir numpy && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ + # It's annoying that this can only be installed via git. + git clone https://github.com/deweylab/RSEM.git && \ cd RSEM && make install && \ rm -rf RSEM @@ -39,6 +32,10 @@ v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ rm -r Salmon* # End Salmon installation. +COPY common/dist/data-refinery-common-* common/ +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) + COPY .boto .boto COPY config/ config/ COPY workers/ . From b2994cb2b1f6b559d99f616945311074b23eb180 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 13 Dec 2022 13:51:53 -0800 Subject: [PATCH 059/220] Update affymetrix image. Revert `ccache` and `crancache` optimizations. --- .../affymetrix/bioc/dependencies.R | 11 ++--- .../affymetrix/install_ensg_pkgs.R | 5 ++- workers/dockerfiles/Dockerfile.affymetrix | 40 ++++++------------- 3 files changed, 21 insertions(+), 35 deletions(-) diff --git a/workers/R/dependencies/affymetrix/bioc/dependencies.R b/workers/R/dependencies/affymetrix/bioc/dependencies.R index 1570292e0..c6d6a3391 100644 --- a/workers/R/dependencies/affymetrix/bioc/dependencies.R +++ b/workers/R/dependencies/affymetrix/bioc/dependencies.R @@ -1,11 +1,12 @@ -# Turn warnings into errors because biocLite throws warnings instead # of error if it fails to install something. +# Turn warnings into errors because biocLite throws warnings instead +# of error if it fails to install something. options(warn = 2) options(repos = structure(c(CRAN = "https://cloud.r-project.org"))) options(Ncpus = parallel::detectCores()) -# Bioconductor packages, installed by devtools::install_url() +# Bioconductor packages, installed by devtools::install_url(). -# Helper function that installs a list of packages using the input URLs +# Helper function that installs a list of packages using the input URLs. install_with_url <- function(urls) { pkg_ids <- devtools::install_url(urls) if (any(is.na(pkg_ids))) { @@ -34,10 +35,10 @@ bioc_pkg_urls <- c( ) install_with_url(bioc_pkg_urls) -# Invoke another R script to install BrainArray ensg packages +# Invoke another R script to install BrainArray ensg packages. source("install_ensg_pkgs.R") -# Install Bioconductor platform design (pd) packages +# Install Bioconductor platform design (pd) packages. pd_experiment_pkgs <- c( "https://bioconductor.org/packages/3.11/data/experiment/src/contrib/pd.atdschip.tiling_0.26.0.tar.gz" ) diff --git a/workers/R/dependencies/affymetrix/install_ensg_pkgs.R b/workers/R/dependencies/affymetrix/install_ensg_pkgs.R index 5de25a262..dbb1fc516 100644 --- a/workers/R/dependencies/affymetrix/install_ensg_pkgs.R +++ b/workers/R/dependencies/affymetrix/install_ensg_pkgs.R @@ -1,4 +1,5 @@ options(Ncpus = parallel::detectCores()) + install.packages("xml2") library("xml2") ensg_url <- "http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/22.0.0/ensg.asp" @@ -44,5 +45,5 @@ write.table(list(chips, pkg_urls), row.names = FALSE, col.names = FALSE, sep = "\t" ) -# Install these ensg packages -lapply(pkg_urls, devtools::install_url) +# Install these ensg packages. +install_with_url(pkg_urls) diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index e90300240..082163825 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -1,52 +1,36 @@ -FROM ccdlstaging/dr_worker_base:latest +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -# Install and set up ccache. -RUN apt-get update && \ - apt-get install --no-install-recommends -y ccache && \ - for i in c++ cc g++ gcc; do ln -fs /usr/bin/ccache /usr/local/bin/$i; done -COPY workers/ccache.conf /root/.ccache/ccache.conf -ENV PATH="/usr/local/bin:${PATH}" +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip setuptools && \ + # Install this one here instead of via requirements.txt because not + # all processors need it. + pip3 install --ignore-installed --no-cache-dir rpy2==3.4.5 && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt COPY workers/R/dependencies/affymetrix/cran/dependencies.R dependencies.R RUN Rscript dependencies.R COPY workers/R/dependencies/affymetrix/install_ensg_pkgs.R . - COPY workers/R/dependencies/affymetrix/bioc/dependencies.R dependencies.R RUN Rscript dependencies.R -RUN pip3 install --ignore-installed --upgrade pip setuptools && \ - # Install this one here instead of via requirements.txt because not - # all processors need it. - pip3 install --ignore-installed rpy2==3.4.5 - # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip3 cache. - rm -r /root/.cache +COPY .boto .boto +COPY config/ config/ +COPY workers/ . ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION -# Print compiler cache stats (the cache hit ratio should be ~90%). -RUN ccache --show-stats && \ - # Clear out the ccache. - ccache --clear - USER user -COPY .boto .boto -COPY config/ config/ -COPY workers/ . - ENTRYPOINT [] From 37c3a40c71f2c13ba8e9994e4cb561018c6d0ba4 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 18 Jan 2023 17:33:39 -0800 Subject: [PATCH 060/220] Migrate R dependencies to renv. --- common/dockerfiles/Dockerfile.base | 11 +- workers/R/dependencies/affymetrix/renv.R | 361 + workers/R/dependencies/affymetrix/renv.lock | 5958 +++++++++++++++++ workers/R/dependencies/compendia/renv.R | 12 + workers/R/dependencies/compendia/renv.lock | 1070 +++ workers/R/dependencies/downloaders/renv.R | 4 + workers/R/dependencies/downloaders/renv.lock | 1029 +++ workers/R/dependencies/illumina/renv.R | 49 + workers/R/dependencies/illumina/renv.lock | 1615 +++++ .../R/dependencies}/install_devtools.R | 0 .../R/dependencies/install_devtools_renv.R | 76 + workers/R/dependencies/no_op/renv.R | 48 + workers/R/dependencies/no_op/renv.lock | 1389 ++++ workers/R/dependencies/salmon/renv.R | 26 + workers/R/dependencies/salmon/renv.lock | 1064 +++ workers/R/dependencies/smasher/renv.R | 12 + workers/R/dependencies/smasher/renv.lock | 1066 +++ workers/R/renv_load.R | 13 + workers/R/renv_save.R | 12 + workers/dockerfiles/Dockerfile.affymetrix | 45 +- workers/dockerfiles/Dockerfile.compendia | 13 +- workers/dockerfiles/Dockerfile.downloaders | 5 +- workers/dockerfiles/Dockerfile.illumina | 9 +- workers/dockerfiles/Dockerfile.no_op | 8 +- workers/dockerfiles/Dockerfile.salmon | 9 +- workers/dockerfiles/Dockerfile.smasher | 8 +- 26 files changed, 13843 insertions(+), 69 deletions(-) create mode 100644 workers/R/dependencies/affymetrix/renv.R create mode 100644 workers/R/dependencies/affymetrix/renv.lock create mode 100644 workers/R/dependencies/compendia/renv.R create mode 100644 workers/R/dependencies/compendia/renv.lock create mode 100644 workers/R/dependencies/downloaders/renv.R create mode 100644 workers/R/dependencies/downloaders/renv.lock create mode 100644 workers/R/dependencies/illumina/renv.R create mode 100644 workers/R/dependencies/illumina/renv.lock rename {common/R => workers/R/dependencies}/install_devtools.R (100%) create mode 100644 workers/R/dependencies/install_devtools_renv.R create mode 100644 workers/R/dependencies/no_op/renv.R create mode 100644 workers/R/dependencies/no_op/renv.lock create mode 100644 workers/R/dependencies/salmon/renv.R create mode 100644 workers/R/dependencies/salmon/renv.lock create mode 100644 workers/R/dependencies/smasher/renv.R create mode 100644 workers/R/dependencies/smasher/renv.lock create mode 100644 workers/R/renv_load.R create mode 100644 workers/R/renv_save.R diff --git a/common/dockerfiles/Dockerfile.base b/common/dockerfiles/Dockerfile.base index 5b833cef9..a5ca53616 100644 --- a/common/dockerfiles/Dockerfile.base +++ b/common/dockerfiles/Dockerfile.base @@ -36,9 +36,15 @@ RUN apt-get update && \ libcairo-dev \ libcurl4-gnutls-dev \ libedit-dev \ + libfreetype6-dev \ + libfribidi-dev \ libgit2-dev \ + libharfbuzz-dev \ + libjpeg-dev \ + libpng-dev \ libpq-dev \ libssl-dev \ + libtiff5-dev \ libxml2-dev \ llvm-10-dev \ lsb-release \ @@ -59,7 +65,6 @@ RUN apt-get update && \ useradd --create-home --home-dir /home/user/ -g user user && \ chown -R user /home/user/ -# Pre-install dev tools. ENV R_LIBS=/usr/local/lib/R/site-library -COPY common/R/install_devtools.R . -RUN Rscript install_devtools.R + +ENTRYPOINT [] diff --git a/workers/R/dependencies/affymetrix/renv.R b/workers/R/dependencies/affymetrix/renv.R new file mode 100644 index 000000000..2e7fb397b --- /dev/null +++ b/workers/R/dependencies/affymetrix/renv.R @@ -0,0 +1,361 @@ +renv::install("bit@1.1-14") +renv::install("ff@2.2-14") +renv::install("XML@3.98-1.10") +renv::install("bitops@1.0-6") +renv::install("RCurl@1.95-4.10") +renv::install("pkgconfig@2.0.1") +renv::install("backports@1.1.4") +renv::install("rlang@0.4.0") +renv::install("zeallot@0.1.0") +renv::install("glue@1.3.1") +renv::install("digest@0.6.19") +renv::install("vctrs@0.1.0") +renv::install("magrittr@1.5") +renv::install("assertthat@0.2.1") +renv::install("prettyunits@1.0.2") +renv::install("blob@1.1.1") +renv::install("memoise@1.1.0") +renv::install("DBI@1.0.0") +renv::install("Rcpp@1.0.1") +renv::install("bit64@0.9-7") +renv::install("RSQLite@2.0") +renv::install("fansi@0.4.0") +renv::install("crayon@1.3.4") +renv::install("cli@1.1.0") +renv::install("utf8@1.1.4") +renv::install("pillar@1.4.2") +renv::install("lifecycle@1.0.3") +renv::install("tibble@1.4.2") +renv::install("xtable@1.8-2") +renv::install("purrr@0.3.3") +renv::install("tidyselect@0.2.5") +renv::install("R6@2.4.0") +renv::install("dplyr@1.0.2") +renv::install("stringi@1.4.3") +renv::install("tidyr@0.8.2") +renv::install("sys@3.2") +renv::install("askpass@1.1") +renv::install("openssl@1.4") +renv::install("curl@3.3") +renv::install("mime@0.7") +renv::install("httr@1.4.1") +renv::install("hms@0.4.2") +renv::install("clipr@0.6.0") +renv::install("readr@1.3.1") +renv::install("xml2@1.2.0") +renv::install("locfit@1.5-9.4") + +renv::install( + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/GEOquery_2.56.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/SCAN.UPC_2.30.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/affy_1.66.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/genefilter_1.70.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/sva_3.36.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz" +) + +renv::install( + "https://mbni.org/customcdf/22.0.0/ensg.download/bovinebtensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/bovgene10stbtensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/bovgene11stbtensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/celegansceensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/elegene10stceensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/elegene11stceensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/canine2cfensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/canine2pmcfensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/cangene10stcfensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/cangene11stcfensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/zebrafishdrensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/zebgene10stdrensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/zebgene11stdrensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/drosgenome1dmensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/drosophila2dmensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/drogene10stdmensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/drogene11stdmensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/equgene10steqcaensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/equgene11steqcaensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/chickenggensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/chigene10stggensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/chigene11stggensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/clariomdhumanhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/clariomshumanhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/clariomshumanhthsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hcg110hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgfocushsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu133ahsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu133a2hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu133bhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu133plus2hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu219hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu95ahsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu95av2hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu95bhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu95chsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu95dhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hgu95ehsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hthgu133ahsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hthgu133bhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hthgu133pluspmhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hu6800hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/jnj384screenhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/mapping10kxba142hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/mapping250knsphsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/mapping250kstyhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/mapping50khind240hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/mapping50kxba240hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/nugohs1a520180hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/primeviewhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/u133aaofav2hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/u133x3phsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/xcelhsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/gngnf1bahsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hglue20hsensgprobe_22.0.0.tar.gz", + "https://mbni.org/customcdf/22.0.0/ensg.download/hta20hsensgprobe_22.0.0.tar.gz", + 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+renv::install("remotes@2.2.0") +renv::install("R6@2.4.1") +renv::install("praise@1.0.0") +renv::install("magrittr@1.5") +renv::install("digest@0.6.25") +renv::install("evaluate@0.14") +renv::install("backports@1.1.8") +renv::install("rprojroot@1.3-2") +renv::install("desc@1.2.0") +renv::install("prettyunits@1.1.1") +renv::install("ps@1.3.3") +renv::install("processx@3.4.3") +renv::install("callr@3.4.3") +renv::install("pkgbuild@1.1.0") +renv::install("rstudioapi@0.11") +renv::install("pkgload@1.1.0") +renv::install("testthat@2.3.2") +renv::install("mime@0.9") +renv::install("sys@3.4") +renv::install("askpass@1.1") +renv::install("openssl@1.4.2") +renv::install("httr@1.4.2") +renv::install("fs@1.4.2") +renv::install("git2r@0.27.1") +renv::install("whisker@0.4") +renv::install("clipr@0.7.0") +renv::install("ini@0.3.1") +renv::install("gh@1.1.0") +renv::install("utf8@1.1.4") +renv::install("lifecycle@0.2.0") +renv::install("vctrs@0.3.2") +renv::install("pillar@1.4.6") +renv::install("pkgconfig@2.0.3") +renv::install("tibble@3.0.3") +renv::install("rematch2@2.1.2") +renv::install("purrr@0.3.4") +renv::install("yaml@2.2.1") +renv::install("usethis@1.6.1") +renv::install("lazyeval@0.2.2") +renv::install("rex@1.2.0") +renv::install("covr@3.5.0") +renv::install("memoise@1.1.0") +renv::install("base64enc@0.1-3") +renv::install("htmltools@0.5.0") +renv::install("crosstalk@1.1.0.1") +renv::install("htmlwidgets@1.5.1") +renv::install("Rcpp@1.0.5") +renv::install("BH@1.72.0-3") +renv::install("later@1.1.0.1") +renv::install("promises@1.1.1") +renv::install("DT@0.14") +renv::install("commonmark@1.7") +renv::install("stringi@1.4.6") +renv::install("stringr@1.4.0") +renv::install("xfun@0.16") +renv::install("markdown@1.1") +renv::install("highr@0.8") +renv::install("knitr@1.29") +renv::install("brew@1.0-6") +renv::install("roxygen2@7.1.1") +renv::install("xopen@1.0.0") +renv::install("rcmdcheck@1.3.3") +renv::install("devtools@2.3.1") diff --git a/workers/R/dependencies/no_op/renv.R b/workers/R/dependencies/no_op/renv.R new file mode 100644 index 000000000..04fcf2910 --- /dev/null +++ b/workers/R/dependencies/no_op/renv.R @@ -0,0 +1,48 @@ +renv::install("data.table@1.11.0") +renv::install("getopt@1.20.3") +renv::install("optparse@1.4.4") +renv::install("rlang@0.3.1") +renv::install("crayon@1.3.4") +renv::install("assertthat@0.2.1") +renv::install("cli@1.1.0") +renv::install("fansi@0.4.0") +renv::install("utf8@1.1.4") +renv::install("digest@0.6.18") +renv::install("rlang@0.3.1") +renv::install("glue@1.3.1") +renv::install("pillar@1.3.1") +renv::install("pkgconfig@2.0.2") +renv::install("pillar@1.3.1") +renv::install("tibble@2.0.1") +renv::install("lazyeval@0.2.1") +renv::install("Rcpp@1.0.1") +renv::install("magrittr@1.5") +renv::install("purrr@0.3.3") +renv::install("tidyselect@0.2.5") +renv::install("R6@2.4.0") +renv::install("lifecycle@1.0.3") +renv::install("tibble@2.0.1") +renv::install("dplyr@1.0.2") 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+renv::install("glue@1.3.1") +renv::install("digest@0.6.19") +renv::install("vctrs@0.1.0") +renv::install("hms@0.4.2") +renv::install("Rcpp@1.0.1") +renv::install("assertthat@0.2.1") +renv::install("crayon@1.3.4") +renv::install("cli@1.1.0") +renv::install("utf8@1.1.4") +renv::install("fansi@0.4.0") +renv::install("pillar@1.4.2") +renv::install("tibble@2.1.3") +renv::install("readr@1.1.1") + +renv::install( + "https://bioconductor.org/packages/3.11/bioc/src/contrib/tximport_1.16.1.tar.gz" +) diff --git a/workers/R/dependencies/salmon/renv.lock b/workers/R/dependencies/salmon/renv.lock new file mode 100644 index 000000000..8ce773ae5 --- /dev/null +++ b/workers/R/dependencies/salmon/renv.lock @@ -0,0 +1,1064 @@ +{ + "R": { + "Version": "3.6.3", + "Repositories": [ + { + "Name": "BioconductorAnnotation", + "URL": "https://bioconductor.org/packages/3.11/data/annotation" + }, + { + "Name": "BioconductorExperiment", + "URL": "https://bioconductor.org/packages/3.11/data/experiment" + }, + { + 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b/workers/R/dependencies/smasher/renv.R @@ -0,0 +1,12 @@ +renv::install("codetools@0.2-15") +renv::install("iterators@1.0.10") +renv::install("foreach@1.4.4") +renv::install("doParallel@1.0.11") +renv::install("data.table@1.11.0") +renv::install("getopt@1.20.3") +renv::install("optparse@1.4.4") +renv::install("lazyeval@0.2.1") + +renv::install( + "https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz" +) diff --git a/workers/R/dependencies/smasher/renv.lock b/workers/R/dependencies/smasher/renv.lock new file mode 100644 index 000000000..9a191bf8e --- /dev/null +++ b/workers/R/dependencies/smasher/renv.lock @@ -0,0 +1,1066 @@ +{ + "R": { + "Version": "3.6.3", + "Repositories": [ + { + "Name": "BioconductorAnnotation", + "URL": "https://bioconductor.org/packages/3.11/data/annotation" + }, + { + "Name": "BioconductorExperiment", + "URL": "https://bioconductor.org/packages/3.11/data/experiment" + }, + { + "Name": "CRAN", + "URL": "https://cloud.r-project.org" 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"Requirements": [] + }, + "rversions": { + "Package": "rversions", + "Version": "2.0.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "0ec41191f744d0f5afad8c6f35cc36e4", + "Requirements": [ + "curl", + "xml2" + ] + }, + "sessioninfo": { + "Package": "sessioninfo", + "Version": "1.1.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "308013098befe37484df72c39cf90d6e", + "Requirements": [ + "cli", + "withr" + ] + }, + "spatial": { + "Package": "spatial", + "Version": "7.3-11", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "f27a078d834614513608da2c08031130", + "Requirements": [] + }, + "stringi": { + "Package": "stringi", + "Version": "1.4.6", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "e99d8d656980d2dd416a962ae55aec90", + "Requirements": [] + }, + "stringr": { + "Package": "stringr", + "Version": "1.4.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "0759e6b6c0957edb1311028a49a35e76", + "Requirements": [ + "glue", + "magrittr", + "stringi" + ] + }, + "survival": { + "Package": "survival", + "Version": "3.4-0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "04411ae66ab4659230c067c32966fc20", + "Requirements": [ + "Matrix" + ] + }, + "sys": { + "Package": "sys", + "Version": "3.4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "b227d13e29222b4574486cfcbde077fa", + "Requirements": [] + }, + "testthat": { + "Package": "testthat", + "Version": "2.3.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "0829b987b8961fb07f3b1b64a2fbc495", + "Requirements": [ + "R6", + "cli", + "crayon", + "digest", + "ellipsis", + "evaluate", + "magrittr", + "pkgload", + "praise", + "rlang", + "withr" + ] + }, + "tibble": { + "Package": "tibble", + "Version": "3.0.3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "08bd36bd34b20d4f7971d49e81deaab0", + "Requirements": [ + "cli", + "crayon", + "ellipsis", + "fansi", + "lifecycle", + "magrittr", + "pillar", + "pkgconfig", + "rlang", + "vctrs" + ] + }, + "usethis": { + "Package": "usethis", + "Version": "1.6.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "e1985f5a9985fea2e338fa7eb99018ca", + "Requirements": [ + "cli", + "clipr", + "crayon", + "curl", + "desc", + "fs", + "gh", + "git2r", + "glue", + "purrr", + "rematch2", + "rlang", + "rprojroot", + "rstudioapi", + "whisker", + "withr", + "yaml" + ] + }, + "utf8": { + "Package": "utf8", + "Version": "1.1.4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "4a5081acfb7b81a572e4384a7aaf2af1", + "Requirements": [] + }, + "vctrs": { + "Package": "vctrs", + "Version": "0.3.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "5ba3006888ac62fd5e97b208d00d3317", + "Requirements": [ + "digest", + "ellipsis", + "glue", + "rlang" + ] + }, + "whisker": { + "Package": "whisker", + "Version": "0.4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "ca970b96d894e90397ed20637a0c1bbe", + "Requirements": [] + }, + "withr": { + "Package": "withr", + "Version": "2.2.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "ecd17882a0b4419545691e095b74ee89", + "Requirements": [] + }, + "xfun": { + "Package": "xfun", + "Version": "0.16", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "b4106139b90981a8bfea9c10bab0baf1", + "Requirements": [] + }, + "xml2": { + "Package": "xml2", + "Version": "1.3.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "d4d71a75dd3ea9eb5fa28cc21f9585e2", + "Requirements": [] + }, + "xopen": { + "Package": "xopen", + "Version": "1.0.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "6c85f015dee9cc7710ddd20f86881f58", + "Requirements": [ + "processx" + ] + }, + "yaml": { + "Package": "yaml", + "Version": "2.2.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "2826c5d9efb0a88f657c7a679c7106db", + "Requirements": [] + } + } +} diff --git a/workers/R/renv_load.R b/workers/R/renv_load.R new file mode 100644 index 000000000..abaf13935 --- /dev/null +++ b/workers/R/renv_load.R @@ -0,0 +1,13 @@ +options(warn = 2) +options(repos = structure(c( + Bioconductor = "https://bioconductor.org/packages/3.11/bioc/", + BioconductorAnnotation = "https://bioconductor.org/packages/3.11/data/annotation/", + BioconductorExperiment = "https://bioconductor.org/packages/3.11/data/experiment", + CRAN = "https://cloud.r-project.org/" +))) +options(Ncpus = parallel::detectCores()) + +install.packages("renv") + +renv::consent(provided = TRUE) +renv::restore(prompt = FALSE) diff --git a/workers/R/renv_save.R b/workers/R/renv_save.R new file mode 100644 index 000000000..5857f391b --- /dev/null +++ b/workers/R/renv_save.R @@ -0,0 +1,12 @@ +options(warn = 2) +options(repos = structure(c( + Bioconductor = "https://bioconductor.org/packages/3.11/bioc/", + BioconductorAnnotation = "https://bioconductor.org/packages/3.11/data/annotation/", + BioconductorExperiment = "https://bioconductor.org/packages/3.11/data/experiment", + CRAN = "https://cloud.r-project.org/" +))) +options(Ncpus = parallel::detectCores()) + +install.packages("renv") + +renv::init(bare = TRUE) diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index e90300240..14bf633a7 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -1,52 +1,33 @@ -FROM ccdlstaging/dr_worker_base:latest +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -# Install and set up ccache. -RUN apt-get update && \ - apt-get install --no-install-recommends -y ccache && \ - for i in c++ cc g++ gcc; do ln -fs /usr/bin/ccache /usr/local/bin/$i; done -COPY workers/ccache.conf /root/.ccache/ccache.conf -ENV PATH="/usr/local/bin:${PATH}" +COPY workers/R/dependencies/affymetrix/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R -COPY workers/R/dependencies/affymetrix/cran/dependencies.R dependencies.R -RUN Rscript dependencies.R - -COPY workers/R/dependencies/affymetrix/install_ensg_pkgs.R . - -COPY workers/R/dependencies/affymetrix/bioc/dependencies.R dependencies.R -RUN Rscript dependencies.R - -RUN pip3 install --ignore-installed --upgrade pip setuptools && \ +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip setuptools && \ # Install this one here instead of via requirements.txt because not # all processors need it. - pip3 install --ignore-installed rpy2==3.4.5 + pip3 install --ignore-installed --no-cache-dir rpy2==3.4.5 && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip3 cache. - rm -r /root/.cache +COPY .boto .boto +COPY config/ config/ +COPY workers/ . ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION -# Print compiler cache stats (the cache hit ratio should be ~90%). -RUN ccache --show-stats && \ - # Clear out the ccache. - ccache --clear - USER user -COPY .boto .boto -COPY config/ config/ -COPY workers/ . - ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 8790d859c..d49e7918d 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -70,18 +70,11 @@ RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ WORKDIR /home/user -# We need a few special packages for QN. ENV R_LIBS=/usr/local/lib/R/site-library -COPY common/R/install_devtools.R . -RUN Rscript install_devtools.R - -COPY workers/R/dependencies/qn/cran/dependencies.R . -RUN Rscript dependencies.R - -COPY workers/R/dependencies/qn/bioc/dependencies.R . -RUN Rscript dependencies.R -# End QN-specific. +COPY workers/R/dependencies/compendia/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 9b611956d..534084860 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -5,8 +5,9 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -COPY workers/R/dependencies/install_downloader_R_only.R . -RUN Rscript install_downloader_R_only.R +COPY workers/R/dependencies/downloaders/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/downloaders/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index 200b37725..4bbbf9ea7 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -5,12 +5,9 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -COPY workers/R/dependencies/illumina/cran/dependencies.R . -RUN Rscript dependencies.R - -# These are for Illumina. -COPY workers/R/dependencies/illumina/bioc/dependencies.R . -RUN Rscript dependencies.R +COPY workers/R/dependencies/illumina/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index 60f686265..a46c45fe8 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -5,11 +5,9 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -COPY workers/R/dependencies/no_op/cran/dependencies.R . -RUN Rscript dependencies.R - -COPY workers/R/dependencies/no_op/install_gene_convert.R . -RUN Rscript install_gene_convert.R +COPY workers/R/dependencies/no_op/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index 046ee0b89..af93dbcb3 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -5,12 +5,9 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -COPY workers/R/dependencies/tximport/cran/dependencies.R tximport_dependencies.R -RUN Rscript tximport_dependencies.R - -# Install tximport. -COPY workers/R/dependencies/tximport/install_tximport.R . -RUN Rscript install_tximport.R +COPY workers/R/dependencies/salmon/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index 926f98208..78258cf01 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -5,11 +5,9 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -COPY workers/R/dependencies/qn/cran/dependencies.R . -RUN Rscript dependencies.R - -COPY workers/R/dependencies/qn/bioc/dependencies.R . -RUN Rscript dependencies.R +COPY workers/R/dependencies/smasher/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ From f9b6d7ea4ad7e0f6a193da7ff9ef022a933e48e6 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 23 Jan 2023 10:59:52 -0800 Subject: [PATCH 061/220] Fix `affymetrix_local` permission denied issue. --- workers/dockerfiles/Dockerfile.affymetrix_local | 2 ++ 1 file changed, 2 insertions(+) diff --git a/workers/dockerfiles/Dockerfile.affymetrix_local b/workers/dockerfiles/Dockerfile.affymetrix_local index 08db9e197..b4cab3676 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix_local +++ b/workers/dockerfiles/Dockerfile.affymetrix_local @@ -3,6 +3,8 @@ FROM ccdlstaging/dr_affymetrix:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] +USER root + WORKDIR /home/user # Remove the version of common already installed. From 85aed7be0be5b6838403e0031bf6a646382144cb Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 26 Jan 2023 08:20:17 -0800 Subject: [PATCH 062/220] Migrate `common_tests` image. --- common/R/dependencies/common_tests/renv.R | 24 + common/R/dependencies/common_tests/renv.lock | 1808 ++++++++++++++++++ common/R/renv_load.R | 13 + common/dockerfiles/Dockerfile.common_tests | 82 +- 4 files changed, 1862 insertions(+), 65 deletions(-) create mode 100644 common/R/dependencies/common_tests/renv.R create mode 100644 common/R/dependencies/common_tests/renv.lock create mode 100644 common/R/renv_load.R diff --git a/common/R/dependencies/common_tests/renv.R b/common/R/dependencies/common_tests/renv.R new file mode 100644 index 000000000..b5a23e262 --- /dev/null +++ b/common/R/dependencies/common_tests/renv.R @@ -0,0 +1,24 @@ +renv::install("rlang@1.0.4") +renv::install("dplyr@1.0.0") +renv::install("tidyr@1.1.0") +renv::install("ff@2.2-14") +renv::install("locfit@1.5-9.4") +renv::install("cli@3.3.0") +renv::install("lifecycle@1.0.3") +renv::install("vctrs@0.4.1") + +renv::install( + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/GEOquery_2.56.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/SCAN.UPC_2.30.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/affy_1.66.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/genefilter_1.70.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/sva_3.36.0.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/tximport_1.16.1.tar.gz", + "https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz" +) diff --git a/common/R/dependencies/common_tests/renv.lock b/common/R/dependencies/common_tests/renv.lock new file mode 100644 index 000000000..a715ce7d2 --- /dev/null +++ b/common/R/dependencies/common_tests/renv.lock @@ -0,0 +1,1808 @@ +{ + "R": { + "Version": "3.6.3", + "Repositories": [ + { + "Name": "Bioconductor", + "URL": "https://bioconductor.org/packages/3.11/bioc" + }, + { + "Name": "BioconductorAnnotation", + "URL": "https://bioconductor.org/packages/3.11/data/annotation" + }, + { + "Name": "BioconductorExperiment", + "URL": "https://bioconductor.org/packages/3.11/data/experiment" + }, + { + "Name": "CRAN", + "URL": "https://cloud.r-project.org" + } + ] + }, + "Packages": { + "AnnotationDbi": { + "Package": "AnnotationDbi", + "Version": 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"Hash": "b4106139b90981a8bfea9c10bab0baf1", + "Requirements": [] + }, + "xml2": { + "Package": "xml2", + "Version": "1.3.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "d4d71a75dd3ea9eb5fa28cc21f9585e2", + "Requirements": [] + }, + "xopen": { + "Package": "xopen", + "Version": "1.0.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "6c85f015dee9cc7710ddd20f86881f58", + "Requirements": [ + "processx" + ] + }, + "xtable": { + "Package": "xtable", + "Version": "1.8-4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "b8acdf8af494d9ec19ccb2481a9b11c2", + "Requirements": [] + }, + "yaml": { + "Package": "yaml", + "Version": "2.2.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "2826c5d9efb0a88f657c7a679c7106db", + "Requirements": [] + }, + "zlibbioc": { + "Package": "zlibbioc", + "Version": "1.34.0", + "Source": "URL", + "RemoteType": "url", + "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz", + "git_url": "https://git.bioconductor.org/packages/zlibbioc", + "git_branch": "RELEASE_3_11", + "git_last_commit": "2db035c", + "git_last_commit_date": "2020-04-27", + "Hash": "d9bbfbd67e8c4bced2f5c1ce4251a744", + "Requirements": [] + } + } +} diff --git a/common/R/renv_load.R b/common/R/renv_load.R new file mode 100644 index 000000000..abaf13935 --- /dev/null +++ b/common/R/renv_load.R @@ -0,0 +1,13 @@ +options(warn = 2) +options(repos = structure(c( + Bioconductor = "https://bioconductor.org/packages/3.11/bioc/", + BioconductorAnnotation = "https://bioconductor.org/packages/3.11/data/annotation/", + BioconductorExperiment = "https://bioconductor.org/packages/3.11/data/experiment", + CRAN = "https://cloud.r-project.org/" +))) +options(Ncpus = parallel::detectCores()) + +install.packages("renv") + +renv::consent(provided = TRUE) +renv::restore(prompt = FALSE) diff --git a/common/dockerfiles/Dockerfile.common_tests b/common/dockerfiles/Dockerfile.common_tests index da18704b2..015e89bb6 100644 --- a/common/dockerfiles/Dockerfile.common_tests +++ b/common/dockerfiles/Dockerfile.common_tests @@ -1,79 +1,31 @@ -FROM ubuntu:18.04 +FROM ccdlstaging/dr_base:latest -RUN apt-get update -qq -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN apt-get update -qq -RUN apt-get -y install apt-fast - -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - -# The packages related to R are somewhat weird, see the README for more details. - -COPY workers/CRAN.gpg . -RUN \ - apt-fast update -qq && \ - apt-get install -y apt-transport-https && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq && \ - apt-fast install -y \ - ed \ - git \ - python3 \ - python3-pip \ - python3-dev \ - r-base-core \ - r-base-dev \ - libpq-dev \ - libxml2-dev \ - libssl-dev \ - libcurl4-openssl-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* -RUN rm CRAN.gpg - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user -WORKDIR /home/user - -ENV R_LIBS "/usr/local/lib/R/site-library" - -COPY common/install_devtools.R . - -RUN Rscript install_devtools.R - -COPY workers/install_bioc.R . - -RUN Rscript install_bioc.R - -COPY workers/install_affy_only.R . - -RUN Rscript install_affy_only.R +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] # Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 # For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 +ENV LANG=C.UTF-8 +ENV R_LIBS=/usr/local/lib/R/site-library -COPY config config - -COPY common/requirements.txt . +WORKDIR /home/user -RUN pip3 install --no-cache-dir -r requirements.txt +COPY common/R/dependencies/common_tests/renv.lock . +COPY common/R/renv_load.R . +RUN Rscript renv_load.R -# Install this rpy2 here instead of via requirements.txt because -# pip-compile throws an error for it. -RUN pip3 install rpy2==3.4.5 +COPY common/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ + # Install this rpy2 here instead of via requirements.txt because + # pip-compile throws an error for it. + pip3 install --ignore-installed --no-cache-dir rpy2==3.4.5 COPY common/ . +COPY config config ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -ENTRYPOINT [""] +ENTRYPOINT [] From 6ec7fa6731b8580f8491baaf41a37250a07b8e44 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 2 Feb 2023 10:01:41 -0800 Subject: [PATCH 063/220] Fix cache disk usage. --- workers/R/renv_load.R | 3 ++- workers/R/renv_save.R | 1 + workers/dockerfiles/Dockerfile.affymetrix | 2 ++ workers/dockerfiles/Dockerfile.affymetrix_local | 2 ++ workers/dockerfiles/Dockerfile.compendia | 2 ++ workers/dockerfiles/Dockerfile.downloaders | 2 ++ workers/dockerfiles/Dockerfile.illumina | 2 ++ workers/dockerfiles/Dockerfile.no_op | 2 ++ workers/dockerfiles/Dockerfile.salmon | 2 ++ workers/dockerfiles/Dockerfile.smasher | 2 ++ workers/dockerfiles/Dockerfile.transcriptome | 2 ++ 11 files changed, 21 insertions(+), 1 deletion(-) diff --git a/workers/R/renv_load.R b/workers/R/renv_load.R index abaf13935..8468d72ed 100644 --- a/workers/R/renv_load.R +++ b/workers/R/renv_load.R @@ -6,8 +6,9 @@ options(repos = structure(c( CRAN = "https://cloud.r-project.org/" ))) options(Ncpus = parallel::detectCores()) +options(renv.settings.use.cache = FALSE) install.packages("renv") renv::consent(provided = TRUE) -renv::restore(prompt = FALSE) +renv::restore(prompt = FALSE, rebuild = TRUE) diff --git a/workers/R/renv_save.R b/workers/R/renv_save.R index 5857f391b..6650cd06b 100644 --- a/workers/R/renv_save.R +++ b/workers/R/renv_save.R @@ -6,6 +6,7 @@ options(repos = structure(c( CRAN = "https://cloud.r-project.org/" ))) options(Ncpus = parallel::detectCores()) +options(renv.settings.use.cache = FALSE) install.packages("renv") diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index 14bf633a7..617ab1706 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -25,6 +25,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.affymetrix_local b/workers/dockerfiles/Dockerfile.affymetrix_local index b4cab3676..7fe881ab0 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix_local +++ b/workers/dockerfiles/Dockerfile.affymetrix_local @@ -19,6 +19,8 @@ RUN pip3 install --ignore-installed --no-cache-dir \ COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index d49e7918d..78b88af37 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -99,6 +99,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 534084860..b8f8a3082 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -41,6 +41,8 @@ COPY .boto .boto COPY config config COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index 4bbbf9ea7..50c72d8fe 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -24,6 +24,8 @@ COPY workers/ . COPY workers/data_refinery_workers/processors/detect_database.R . COPY workers/illumina_probe_maps/ probe_maps/ +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index a46c45fe8..ca8c33202 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -37,6 +37,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index af93dbcb3..c10b5e290 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -58,6 +58,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index 78258cf01..1c9905b2a 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -23,6 +23,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index f7dbe3676..69020d49b 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -40,6 +40,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION From 29b8d24e7f8db875c92fa785b70730ffdaa7834a Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 2 Feb 2023 10:25:52 -0800 Subject: [PATCH 064/220] Rename cron job script. --- .../foreman-server-instance-user-data.tpl.sh | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh index 3598c2a45..c53d368bf 100644 --- a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh +++ b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh @@ -77,8 +77,8 @@ docker run \\ -e DATABASE_PASSWORD=${database_password} \\ -v /tmp:/tmp \\ -it ${dockerhub_repo}/dr_\"\$1\" python3 manage.py \"\$2\" -" >> /home/ubuntu/run_cron_job.sh -chmod +x /home/ubuntu/run_cron_job.sh +" >> /home/ubuntu/run_manage_command.sh +chmod +x /home/ubuntu/run_manage_command.sh # Use Monit to ensure the Foreman is always running apt-get -y update @@ -112,9 +112,9 @@ service monit restart # Install the cron job tests crontab -l > tempcron cat <> tempcron -0 12 * * MON /bin/bash /home/ubuntu/run_cron_job.sh affymetrix check_brainarray_gene_agreement >> /var/log/cron_job_tests.log 2>&1 -0 12 * * MON /bin/bash /home/ubuntu/run_cron_job.sh affymetrix check_tx_index_transcript_agreement >> /var/log/cron_job_tests.log 2>&1 -0 12 * * ${accession_gathering_job_run_day} /bin/bash /home/ubuntu/run_cron_job.sh foreman gather_weekly_accessions >> /var/log/gather_weekly_accessions.log 2>&1 +0 12 * * MON /bin/bash /home/ubuntu/run_manage_command.sh affymetrix check_brainarray_gene_agreement >> /var/log/affymetrix_checks.log 2>&1 +0 12 * * MON /bin/bash /home/ubuntu/run_manage_command.sh affymetrix check_tx_index_transcript_agreement >> /var/log/affymetrix_checks.log 2>&1 +0 12 * * ${accession_gathering_job_run_day} /bin/bash /home/ubuntu/run_manage_command.sh foreman gather_weekly_accessions >> /var/log/weekly_accessions.log 2>&1 EOF # install new cron file crontab tempcron From 0698aee72eafe0bf91e1fa0a64b37bb23476b9f7 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 3 Feb 2023 10:26:31 -0800 Subject: [PATCH 065/220] Replace `count()` with `exists()`. --- api/data_refinery_api/views/dataset.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/api/data_refinery_api/views/dataset.py b/api/data_refinery_api/views/dataset.py index 87d4c69ed..9316409d8 100644 --- a/api/data_refinery_api/views/dataset.py +++ b/api/data_refinery_api/views/dataset.py @@ -54,7 +54,7 @@ def experiment_has_downloadable_samples(experiment, quant_sf_only=False): results__computedfile__s3_bucket__isnull=False, ) - if samples.count() == 0: + if not samples.exists(): return False else: @@ -128,7 +128,7 @@ def validate_dataset(data): results__computedfile__s3_key__isnull=False, results__computedfile__s3_bucket__isnull=False, ) - if samples_without_quant_sf.count(): + if samples_without_quant_sf.exists(): raise InvalidData( message="Sample(s) in dataset are missing quant.sf files. See `details` for a full list", details=[s.accession_code for s in samples_without_quant_sf], @@ -136,7 +136,7 @@ def validate_dataset(data): else: unprocessed_samples = samples.exclude(is_processed=True) - if unprocessed_samples.count(): + if unprocessed_samples.exists(): raise InvalidData( message="Non-downloadable sample(s) in dataset. See `details` for a full list", details=[s.accession_code for s in unprocessed_samples], From dca9bf7f44918c0b4014045d421a660cf26b7939 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 7 Feb 2023 14:32:15 -0800 Subject: [PATCH 066/220] Fix merge. --- .../foreman-server-instance-user-data.tpl.sh | 2 -- 1 file changed, 2 deletions(-) diff --git a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh index 050e8f1f1..c53d368bf 100644 --- a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh +++ b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh @@ -112,11 +112,9 @@ service monit restart # Install the cron job tests crontab -l > tempcron cat <> tempcron - 0 12 * * MON /bin/bash /home/ubuntu/run_manage_command.sh affymetrix check_brainarray_gene_agreement >> /var/log/affymetrix_checks.log 2>&1 0 12 * * MON /bin/bash /home/ubuntu/run_manage_command.sh affymetrix check_tx_index_transcript_agreement >> /var/log/affymetrix_checks.log 2>&1 0 12 * * ${accession_gathering_job_run_day} /bin/bash /home/ubuntu/run_manage_command.sh foreman gather_weekly_accessions >> /var/log/weekly_accessions.log 2>&1 - EOF # install new cron file crontab tempcron From 1cf54ad55ad29fd62c7065fb0683855b2e90a46c Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Peter=20St=C3=B6ckli?= Date: Wed, 8 Feb 2023 15:43:41 +0100 Subject: [PATCH 067/220] Fix typo in container registry domain --- scripts/prepare_image.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/scripts/prepare_image.sh b/scripts/prepare_image.sh index e791ce589..81975da90 100755 --- a/scripts/prepare_image.sh +++ b/scripts/prepare_image.sh @@ -104,7 +104,7 @@ else if test "$GITHUB_ACTIONS"; then # docker needs repositories to be lowercase - CACHE_REPO="$(echo "ghrc.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]')" + CACHE_REPO="$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]')" CACHED_PACKAGE="$CACHE_REPO/dr_$image" CACHE="--build-arg BUILDKIT_INLINE_CACHE=1 --cache-from $CACHED_PACKAGE" fi From 231a8e1f20ce3c61936db3b3cd8f0a5f731155b3 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 16 Feb 2023 10:08:15 -0800 Subject: [PATCH 068/220] Address review comments. Fix git unrelated history issue. --- .../commands/post_downloads_summary.py | 196 ++++++++++++------ .../commands/test_post_downloads_summary.py | 128 ++++++++++-- 2 files changed, 237 insertions(+), 87 deletions(-) diff --git a/api/data_refinery_api/management/commands/post_downloads_summary.py b/api/data_refinery_api/management/commands/post_downloads_summary.py index 30a49cb1f..75db2db9b 100644 --- a/api/data_refinery_api/management/commands/post_downloads_summary.py +++ b/api/data_refinery_api/management/commands/post_downloads_summary.py @@ -1,7 +1,9 @@ import datetime +from collections import Counter from django.conf import settings from django.core.management.base import BaseCommand +from django.template.defaultfilters import pluralize from django.utils import timezone import requests @@ -9,109 +11,173 @@ from data_refinery_common.models import Dataset, DatasetAnnotation -def post_downloads_summary(days, channel): - start_time = timezone.now() - datetime.timedelta(days=days) +class Command(BaseCommand): + help = "Post downloads summary to Slack" + def add_arguments(self, parser): + parser.add_argument( + "--channel", + type=str, + default="ccdl-general", + help=("Optional parameter to choose the channel where the message will be posted."), + ) + parser.add_argument( + "--days", + type=int, + default=7, # Default to a week. + help=("Number of days in the past for which to build the stats."), + ) + parser.add_argument( + "--top-countries", + type=int, + default=5, + help=("Number of countries to show in the per country downloads summary."), + ) + + def handle(self, *args, **options): + post_downloads_summary(options["days"], options["channel"], options["top_countries"]) + + +def format_user_data(header, data): + """ + Formats user email, downloads count, location information sorted + by downloads count. + """ + # Allowed overhead for 2 column sorting: downloads count, email. + lines = sorted(data, key=lambda u: u[0].lower()) + lines = [ + f"{email.lower()} | {downloads} download{pluralize(downloads)} from {location}" + for email, downloads, location in sorted(lines, key=lambda u: u[1], reverse=True) + ] + lines.insert(0, header) + + return "\n".join(lines) + + +def get_user_location(ip_address): + """Gets user location information based on their IP address.""" + try: + data = requests.get(f"https://ipapi.co/{ip_address}/json/", timeout=10).json() + # The list of available fields https://ipapi.co/api/#complete-location + return ", ".join((data["city"], data["country_name"])) + except (requests.exceptions.RequestException, KeyError, ValueError): + return ip_address + + +def post_downloads_summary(days, channel, top_countries=5): + """Posts downloads summary to Slack channel.""" + + start_time = timezone.now() - datetime.timedelta(days=days) datasets = Dataset.processed_filtered_objects.filter( created_at__gt=start_time ).prefetch_related("datasetannotation_set") + annotations = DatasetAnnotation.objects.filter(dataset__in=datasets) + users_emails = set(dataset.email_address for dataset in datasets) - annotation_queryset = DatasetAnnotation.objects.filter(dataset__in=datasets) - - # Save the locations permanently, since IP addresses can cycle over time - location_cache = dict() - for annotation in annotation_queryset: + locations = set() + locations_cache = dict() + for annotation in annotations: if "location" not in annotation.data: - ip = annotation.data["ip"] - if ip not in location_cache: - location_cache[ip] = get_ip_location(ip) - annotation.data["location"] = location_cache[ip] + ip_address = annotation.data["ip"] + if ip_address not in locations_cache: + locations_cache[ip_address] = get_user_location(ip_address) + + # Save the locations permanently, since IP addresses can cycle over time. + annotation.data["location"] = locations_cache[ip_address] annotation.save() + locations.add(annotation.data["location"]) + + downloads_per_country = Counter() + downloads_total = 0 + new_users = [] + returning_users = [] + for user_email in users_emails: + user_annotations = annotations.filter(dataset__email_address=user_email) + user_downloads = user_annotations.count() + downloads_total += user_downloads + + user_locations = set() + for user_annotation in user_annotations: + user_locations.add(annotation.data["location"]) + try: + country = user_annotation.data["location"].split(", ")[1] + downloads_per_country.update({country: 1}) + except (IndexError, TypeError): + pass + + user_locations = "; ".join(sorted(user_locations)) + user_data = (user_email, user_downloads, user_locations) - unique_users = list(set(dataset.email_address for dataset in datasets)) - unique_locations = list(set(annotation.data["location"] for annotation in annotation_queryset)) - - new_users = "" - returning_users = "" - total_downloads = 0 - for email in unique_users: - user_annotations = annotation_queryset.filter(dataset__email_address=email) - downloads = user_annotations.count() - total_downloads += downloads - locations = ", ".join( - list(set(annotation.data["location"] for annotation in user_annotations)) - ) is_returning_user = Dataset.processed_filtered_objects.filter( - created_at__lt=start_time, email_address=email + created_at__lt=start_time, email_address=user_email ) - text = "{0} | {1} downloads from {2}\n".format(email, downloads, locations) if is_returning_user: - returning_users += text + returning_users.append(user_data) else: - new_users += text + new_users.append(user_data) - if total_downloads > 0: + if downloads_total: + locations_count = len(locations) + users_emails_count = len(users_emails) fallback_text = ( - f"In the last {days} days, {len(unique_users)} users downloaded {total_downloads}" - f" datasets from {len(unique_locations)} locations." + f"In the last {days} day{pluralize(days)}, {users_emails_count} " + f"user{pluralize(users_emails_count)} downloaded {downloads_total} " + f"dataset{pluralize(downloads_total)} from {locations_count} " + f"location{pluralize(locations_count)}." ) else: - fallback_text = f"There were no downloads in the last {days} days." + fallback_text = f"There were no downloads in the last {days} day{pluralize(days)}." blocks = [ {"type": "section", "text": {"type": "plain_text", "emoji": True, "text": fallback_text}} ] + if new_users: blocks.append( - {"type": "section", "text": {"type": "mrkdwn", "text": "*New users* \n" + new_users}} + { + "type": "section", + "text": {"type": "mrkdwn", "text": format_user_data("*New users*", new_users)}, + } ) + if returning_users: blocks.append( { "type": "section", - "text": {"type": "mrkdwn", "text": "*Returning users* \n" + returning_users}, + "text": { + "type": "mrkdwn", + "text": format_user_data("*Returning users*", returning_users), + }, + } + ) + + if top_countries and downloads_per_country: + countries_count = downloads_per_country.most_common(top_countries) + top_countries = min(top_countries, len(countries_count)) + lines = [f"*Top {top_countries} countr{pluralize(top_countries, 'y,ies')}*"] + # Allowed overhead for 2 column sorting: downloads count, country. + countries_count = sorted(countries_count, key=lambda cc: cc[0]) + countries_count = sorted(countries_count, key=lambda cc: cc[1], reverse=True) + for country, count in countries_count: + lines.append(f"{country}: {count} download{pluralize(count)}") + + blocks.append( + { + "type": "section", + "text": {"type": "mrkdwn", "text": "\n".join(lines)}, } ) - # Post to slack + # Post to Slack. requests.post( settings.ENGAGEMENTBOT_WEBHOOK, json={ "username": "EngagementBot", "icon_emoji": ":halal:", - "channel": "#" + channel, + "channel": f"#{channel}", "text": fallback_text, "blocks": blocks, }, headers={"Content-Type": "application/json"}, timeout=10, ) - - -class Command(BaseCommand): - help = "Post downloads summary to slack" - - def add_arguments(self, parser): - parser.add_argument( - "--days", - type=int, - default=7, # default to a week - help=("Number of days in the past for which to build the stats"), - ) - parser.add_argument( - "--channel", - type=str, - default="ccdl-general", - help=("Optional parameter to choose the channel where the message will be posted."), - ) - - def handle(self, *args, **options): - post_downloads_summary(options["days"], options["channel"]) - - -def get_ip_location(remote_ip): - try: - data = requests.get("https://ipapi.co/" + remote_ip + "/json/", timeout=10).json() - return "{0}, {1}".format(data["city"], data["country_name"]) - except (requests.exceptions.RequestException, ValueError, KeyError): - return remote_ip diff --git a/api/data_refinery_api/management/commands/test_post_downloads_summary.py b/api/data_refinery_api/management/commands/test_post_downloads_summary.py index f7b00b898..967986b42 100644 --- a/api/data_refinery_api/management/commands/test_post_downloads_summary.py +++ b/api/data_refinery_api/management/commands/test_post_downloads_summary.py @@ -9,51 +9,135 @@ class DownloadsPostTestCase(TestCase): - @patch("data_refinery_common.models.organism.requests.post") + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") def test_post_download_summary_one_download(self, requests_post): dataset = Dataset(email_address="test@gmail.com", is_processed=True) dataset.save() - DatasetAnnotation(dataset=dataset, data={"location": "Testyville"}).save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() - post_downloads_summary(7, "ccdl-general") + post_downloads_summary(7, "ccdl-general-test") + json = requests_post.mock_calls[0][2]["json"] - text = requests_post.mock_calls[0][2]["json"]["text"] - self.assertIn("1 users downloaded 1 datasets from 1 locations.", text) + self.assertEqual( + "In the last 7 days, 1 user downloaded 1 dataset from 1 location.", json["text"] + ) + self.assertEqual( + "\n".join(("*New users*", "test@gmail.com | 1 download from Testyville, TSA")), + json["blocks"][1]["text"]["text"], + ) + self.assertEqual( + "\n".join(("*Top 1 country*", "TSA: 1 download")), json["blocks"][2]["text"]["text"] + ) - @patch("data_refinery_common.models.organism.requests.post") + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") def test_post_download_summary_two_downloads(self, requests_post): dataset = Dataset(email_address="test@gmail.com", is_processed=True) dataset.save() - DatasetAnnotation(dataset=dataset, data={"location": "Testyville"}).save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() + + dataset = Dataset(email_address="test@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() + dataset = Dataset(email_address="test2@gmail.com", is_processed=True) dataset.save() - DatasetAnnotation(dataset=dataset, data={"location": "Not Testyville"}).save() + DatasetAnnotation(dataset=dataset, data={"location": "Not Testyville, Not TSA"}).save() - post_downloads_summary(7, "ccdl-general") + post_downloads_summary(7, "ccdl-general-test") + json = requests_post.mock_calls[0][2]["json"] - text = requests_post.mock_calls[0][2]["json"]["text"] - self.assertIn("2 users downloaded 2 datasets from 2 locations.", text) + self.assertEqual( + "In the last 7 days, 2 users downloaded 3 datasets from 2 locations.", json["text"] + ) + self.assertEqual( + "\n".join( + ( + "*New users*", + "test@gmail.com | 2 downloads from Testyville, TSA", + "test2@gmail.com | 1 download from Not Testyville, Not TSA", + ) + ), + json["blocks"][1]["text"]["text"], + ) + self.assertEqual( + "\n".join(("*Top 2 countries*", "TSA: 2 downloads", "Not TSA: 1 download")), + json["blocks"][2]["text"]["text"], + ) - @patch("data_refinery_common.models.organism.requests.post") + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") + def test_post_download_summary_ordering(self, requests_post): + for _ in range(2): + dataset = Dataset(email_address="ATEST2@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Not Testyville, Not TSA"}).save() + + for _ in range(2): + dataset = Dataset(email_address="TEST@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() + + dataset = Dataset(email_address="TEST3@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Testbec City, Tanada"}).save() + + for _ in range(3): + dataset = Dataset(email_address="WEST@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Westyville, Texico"}).save() + + post_downloads_summary(7, "ccdl-general-test") + json = requests_post.mock_calls[0][2]["json"] + + self.assertEqual( + "In the last 7 days, 4 users downloaded 8 datasets from 4 locations.", json["text"] + ) + self.assertEqual( + "\n".join( + ( + "*New users*", + "west@gmail.com | 3 downloads from Westyville, Texico", + "atest2@gmail.com | 2 downloads from Not Testyville, Not TSA", + "test@gmail.com | 2 downloads from Testyville, TSA", + "test3@gmail.com | 1 download from Testbec City, Tanada", + ) + ), + json["blocks"][1]["text"]["text"], + ) + self.assertEqual( + "\n".join( + ( + "*Top 4 countries*", + "Texico: 3 downloads", + "Not TSA: 2 downloads", + "TSA: 2 downloads", + "Tanada: 1 download", + ) + ), + json["blocks"][2]["text"]["text"], + ) + + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") def test_post_download_summary_filtered(self, requests_post): dataset = Dataset(email_address="test@gmail.com", is_processed=True) dataset.save() - DatasetAnnotation(dataset=dataset, data={"location": "Testyville"}).save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() + last_week = timezone.now() - timedelta(days=8) dataset = Dataset(created_at=last_week, email_address="test2@gmail.com", is_processed=True) dataset.save() DatasetAnnotation( - created_at=last_week, dataset=dataset, data={"location": "Not Testyville"} + created_at=last_week, dataset=dataset, data={"location": "Testyville, TSA"} ).save() - post_downloads_summary(7, "ccdl-general") - - text = requests_post.mock_calls[0][2]["json"]["text"] - self.assertIn("1 users downloaded 1 datasets from 1 locations.", text) + post_downloads_summary(7, "ccdl-general-test") + json = requests_post.mock_calls[0][2]["json"] + self.assertEqual( + "In the last 7 days, 1 user downloaded 1 dataset from 1 location.", json["text"] + ) - @patch("data_refinery_common.models.organism.requests.post") + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") def test_empty_post_download_summary(self, requests_post): - post_downloads_summary(7, "ccdl-general") + post_downloads_summary(7, "ccdl-general-test") - text = requests_post.mock_calls[0][2]["json"]["text"] - self.assertIn("no downloads", text) + json = requests_post.mock_calls[0][2]["json"] + self.assertEqual("There were no downloads in the last 7 days.", json["text"]) From 8ad2452bd158273d08444bb362e3f59409fb0b7a Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 16 Feb 2023 10:15:47 -0800 Subject: [PATCH 069/220] Fix tests. --- .../management/commands/post_downloads_summary.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/api/data_refinery_api/management/commands/post_downloads_summary.py b/api/data_refinery_api/management/commands/post_downloads_summary.py index 75db2db9b..f43650cd5 100644 --- a/api/data_refinery_api/management/commands/post_downloads_summary.py +++ b/api/data_refinery_api/management/commands/post_downloads_summary.py @@ -98,7 +98,7 @@ def post_downloads_summary(days, channel, top_countries=5): user_locations = set() for user_annotation in user_annotations: - user_locations.add(annotation.data["location"]) + user_locations.add(user_annotation.data["location"]) try: country = user_annotation.data["location"].split(", ")[1] downloads_per_country.update({country: 1}) From 273bb3ce8d3ddb3cc59beacc9c57f93ddc33d91f Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 3 Mar 2023 10:33:33 -0800 Subject: [PATCH 070/220] Update Github actions checkout to v3. --- .github/workflows/config.yml | 26 +++++++++++++------------- 1 file changed, 13 insertions(+), 13 deletions(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 1a7b832b0..74113e557 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -10,7 +10,7 @@ jobs: AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - uses: hashicorp/setup-terraform@v1 with: @@ -37,7 +37,7 @@ jobs: DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Free up space run: ./.github/scripts/cleanup_instance.sh @@ -83,7 +83,7 @@ jobs: DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Login to Packages Container registry uses: docker/login-action@v1 @@ -125,7 +125,7 @@ jobs: DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Free up space run: ./.github/scripts/cleanup_instance.sh @@ -177,7 +177,7 @@ jobs: DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Login to Packages Container registry uses: docker/login-action@v1 @@ -226,7 +226,7 @@ jobs: DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Free up space run: ./.github/scripts/cleanup_instance.sh @@ -278,7 +278,7 @@ jobs: DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Login to Packages Container registry uses: docker/login-action@v1 @@ -321,7 +321,7 @@ jobs: DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Login to Packages Container registry uses: docker/login-action@v1 @@ -363,7 +363,7 @@ jobs: DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Free up space run: ./.github/scripts/cleanup_instance.sh @@ -426,7 +426,7 @@ jobs: DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Login to Packages Container registry uses: docker/login-action@v1 @@ -470,7 +470,7 @@ jobs: outputs: branch: ${{ steps.set_branch.outputs.branch }} steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 with: fetch-depth: 0 @@ -515,7 +515,7 @@ jobs: - test_smasher - test_transcriptome steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 with: fetch-depth: 0 @@ -576,7 +576,7 @@ jobs: needs: - determine_branch steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 with: fetch-depth: 0 From 0091e526b702bf65a820c9994b9c957cf28e7a19 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 3 Mar 2023 10:44:23 -0800 Subject: [PATCH 071/220] Update API requirements and run_tests.sh --- api/requirements.in | 4 +- api/requirements.txt | 188 +++++++++++-------------------------------- api/run_tests.sh | 1 + 3 files changed, 52 insertions(+), 141 deletions(-) diff --git a/api/requirements.in b/api/requirements.in index 5382ed624..e167ead49 100644 --- a/api/requirements.in +++ b/api/requirements.in @@ -1,5 +1,5 @@ coverage -django>=3.2.5 +django>=3.2,<4 psycopg2-binary boto3>=1.12.21 requests>=2.23.0 @@ -7,7 +7,7 @@ urllib3>=1.24.2 Jinja2>=2.11.3 # We need at least 1.1 because of https://github.com/pallets/markupsafe/issues/57 markupsafe>=1.1 -djangorestframework>=3.11.2 +djangorestframework<3.14.0 djangorestframework-hstore django-cors-headers django-filter diff --git a/api/requirements.txt b/api/requirements.txt index d3adfa3fa..59317c266 100644 --- a/api/requirements.txt +++ b/api/requirements.txt @@ -1,146 +1,56 @@ # -# This file is autogenerated by pip-compile +# This file is autogenerated by pip-compile with python 3.9 # To update, run: # -# pip-compile requirements.in +# pip-compile --annotation-style=line api/requirements.in # -apscheduler==3.7.0 - # via -r requirements.in -asgiref==3.3.4 - # via django -boto3==1.17.93 - # via -r requirements.in -botocore==1.20.93 - # via - # boto3 - # s3transfer -certifi==2021.5.30 - # via requests -chardet==4.0.0 - # via requests -coreapi==2.3.3 - # via - # -r requirements.in - # drf-yasg -coreschema==0.0.4 - # via - # coreapi - # drf-yasg -coverage==5.5 - # via -r requirements.in -django==3.2.15 - # via - # -r requirements.in - # django-cors-headers - # django-filter - # django-nine - # djangorestframework - # djangorestframework-hstore - # drf-yasg -django-computedfields==0.1.5 - # via -r requirements.in -django-cors-headers==3.7.0 - # via -r requirements.in -django-elasticsearch-dsl==6.5.0 - # via - # -r requirements.in - # django-elasticsearch-dsl-drf -django-elasticsearch-dsl-drf==0.22.1 - # via -r requirements.in -django-filter==2.4.0 - # via -r requirements.in -django-hstore==1.4.2 - # via djangorestframework-hstore -django-nine==0.2.4 - # via django-elasticsearch-dsl-drf -djangorestframework==3.12.4 - # via - # -r requirements.in - # django-elasticsearch-dsl-drf - # djangorestframework-hstore - # drf-yasg -djangorestframework-hstore==1.3 - # via -r requirements.in -drf-yasg==1.20.0 - # via -r requirements.in -elasticsearch==6.8.2 - # via - # django-elasticsearch-dsl-drf - # elasticsearch-dsl -elasticsearch-dsl==6.4.0 - # via - # -r requirements.in - # django-elasticsearch-dsl - # django-elasticsearch-dsl-drf -idna==2.10 - # via requests -inflection==0.5.1 - # via drf-yasg -itypes==1.2.0 - # via coreapi -jinja2==3.0.1 - # via - # -r requirements.in - # coreschema -jmespath==0.10.0 - # via - # boto3 - # botocore -markupsafe==2.0.1 - # via - # -r requirements.in - # jinja2 -packaging==20.9 - # via drf-yasg -psycopg2==2.8.6 - # via djangorestframework-hstore -psycopg2-binary==2.8.6 - # via -r requirements.in -pyparsing==2.4.7 - # via packaging -python-dateutil==2.8.1 - # via - # botocore - # elasticsearch-dsl -pytz==2021.1 - # via - # apscheduler - # django - # tzlocal -pyyaml==5.4.1 - # via -r requirements.in -requests==2.25.1 - # via - # -r requirements.in - # coreapi -ruamel-yaml==0.17.9 - # via drf-yasg -s3transfer==0.4.2 - # via boto3 -six==1.16.0 - # via - # -r requirements.in - # apscheduler - # django-elasticsearch-dsl - # django-elasticsearch-dsl-drf - # elasticsearch-dsl - # python-dateutil -sqlparse==0.4.2 - # via - # -r requirements.in - # django -tzlocal==2.1 - # via apscheduler -uritemplate==3.0.1 - # via - # coreapi - # drf-yasg -urllib3==1.26.5 - # via - # -r requirements.in - # botocore - # elasticsearch - # requests +apscheduler==3.10.0 # via -r api/requirements.in +asgiref==3.6.0 # via django +boto3==1.26.68 # via -r api/requirements.in +botocore==1.29.68 # via boto3, s3transfer +certifi==2022.12.7 # via requests +charset-normalizer==3.0.1 # via requests +coreapi==2.3.3 # via -r api/requirements.in, drf-yasg +coreschema==0.0.4 # via coreapi, drf-yasg +coverage==7.1.0 # via -r api/requirements.in +django==3.2.17 # via -r api/requirements.in, django-computedfields, django-cors-headers, django-fast-update, django-filter, django-nine, djangorestframework, djangorestframework-hstore, drf-yasg +django-computedfields==0.2.1 # via -r api/requirements.in +django-cors-headers==3.13.0 # via -r api/requirements.in +django-elasticsearch-dsl==6.5.0 # via -r api/requirements.in, django-elasticsearch-dsl-drf +django-elasticsearch-dsl-drf==0.22.5 # via -r api/requirements.in +django-fast-update==0.2.1 # via django-computedfields +django-filter==22.1 # via -r api/requirements.in +django-hstore==1.4.2 # via djangorestframework-hstore +django-nine==0.2.7 # via django-elasticsearch-dsl-drf +djangorestframework==3.13.1 # via -r api/requirements.in, django-elasticsearch-dsl-drf, djangorestframework-hstore, drf-yasg +djangorestframework-hstore==1.3 # via -r api/requirements.in +drf-yasg==1.21.5 # via -r api/requirements.in +elasticsearch==6.8.2 # via django-elasticsearch-dsl-drf, elasticsearch-dsl +elasticsearch-dsl==6.4.0 # via -r api/requirements.in, django-elasticsearch-dsl, django-elasticsearch-dsl-drf +idna==3.4 # via requests +inflection==0.5.1 # via drf-yasg +itypes==1.2.0 # via coreapi +jinja2==3.1.2 # via -r api/requirements.in, coreschema +jmespath==1.0.1 # via boto3, botocore +markupsafe==2.1.2 # via -r api/requirements.in, jinja2 +packaging==23.0 # via django-nine, drf-yasg +psycopg2==2.9.5 # via djangorestframework-hstore +psycopg2-binary==2.9.5 # via -r api/requirements.in +python-dateutil==2.8.2 # via botocore, elasticsearch-dsl +pytz==2022.7.1 # via apscheduler, django, djangorestframework, drf-yasg +pytz-deprecation-shim==0.1.0.post0 # via tzlocal +pyyaml==6.0 # via -r api/requirements.in +requests==2.28.2 # via -r api/requirements.in, coreapi +ruamel-yaml==0.17.21 # via drf-yasg +ruamel-yaml-clib==0.2.7 # via ruamel-yaml +s3transfer==0.6.0 # via boto3 +six==1.16.0 # via -r api/requirements.in, apscheduler, django-elasticsearch-dsl, django-elasticsearch-dsl-drf, elasticsearch-dsl, python-dateutil +sqlparse==0.4.3 # via -r api/requirements.in, django +typing-extensions==4.4.0 # via django-computedfields +tzdata==2022.7 # via pytz-deprecation-shim +tzlocal==4.2 # via apscheduler +uritemplate==4.1.1 # via coreapi, drf-yasg +urllib3==1.26.14 # via -r api/requirements.in, botocore, elasticsearch, requests # The following packages are considered to be unsafe in a requirements file: # setuptools diff --git a/api/run_tests.sh b/api/run_tests.sh index aea19826d..fedf4cbbe 100755 --- a/api/run_tests.sh +++ b/api/run_tests.sh @@ -46,5 +46,6 @@ docker run -t $INTERACTIVE \ --add-host=database:"$DB_HOST_IP" \ --add-host=elasticsearch:"$ES_HOST_IP" \ --env-file api/environments/test \ + --platform linux/amd64 \ --volume "$volume_directory":/home/user/data_store \ ccdlstaging/dr_api_local bash -c "$(run_tests_with_coverage "$@")" From 5d151bb5d07ecd83a1101eda57539ec1a7813efe Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 3 Mar 2023 10:54:24 -0800 Subject: [PATCH 072/220] Update Docker base image. Use R v3.4. Remove R dependencies from the base image. --- common/R/install_devtools.R | 124 ----------------------------- common/dockerfiles/Dockerfile.base | 20 +++-- workers/CRAN.gpg | 44 ---------- 3 files changed, 12 insertions(+), 176 deletions(-) delete mode 100644 common/R/install_devtools.R delete mode 100644 workers/CRAN.gpg diff --git a/common/R/install_devtools.R b/common/R/install_devtools.R deleted file mode 100644 index e241152ea..000000000 --- a/common/R/install_devtools.R +++ /dev/null @@ -1,124 +0,0 @@ -# This script installs version 1.13.6 of the R package devtools. - -# Because devtools is the tool which will install specific versions of -# other R packages, it cannot be used it to install itself. However in -# order to ensure there are no dependency conflicts, it also needs to -# pin each version of its dependencies. - -# This script uses install.packages to install all of the dependencies -# for devtools, however install.packages unfortunately does not -# recursively install dependencies for the packages it -# installs. Therefore this script installs every dependency up the -# chain to devtools. It's not ideal, but it guarantees a stable -# install of devtools that won't break when a new version of devtools -# is released. - -# Cranlock was used to find the versions of dependencies to install - -# Treat warnings as errors, set CRAN mirror, and set parallelization: -options(warn = 2) -options(repos = structure(c(CRAN = "https://cloud.r-project.org/"))) -options(Ncpus = parallel::detectCores()) - - -install_package_version <- function(package_name, version) { - # This function install a specific version of a package. - - # However, because the most current version of a package lives in a - # different location than the older versions, we have to check where - # it can be found. - package_tarball <- paste0(package_name, "_", version, ".tar.gz") - package_url <- paste0("https://cloud.r-project.org/src/contrib/", package_tarball) - - # Give CRAN a full minute to timeout since it's not always the most reliable. - curl_result <- system(paste0("curl --head --connect-timeout 60 ", package_url), intern = TRUE) - if (grepl("404", curl_result[1])) { - package_url <- paste0("https://cloud.r-project.org/src/contrib/Archive/", package_name, "/", package_tarball) - - # Make sure the package actually exists in the archive! - curl_result <- system(paste0("curl --head --connect-timeout 120 ", package_url), intern = TRUE) - if (grepl("404", curl_result[1])) { - stop(paste("Package", package_name, "version", version, "does not exist!")) - } - } - - install.packages(package_url, Ncpus = 32) -} - -# Generated using cranlock -install_package_version("jsonlite", "1.7.0") -install_package_version("withr", "2.2.0") -install_package_version("crayon", "1.3.4") -install_package_version("fansi", "0.4.1") -install_package_version("glue", "1.4.1") -install_package_version("assertthat", "0.2.1") -install_package_version("cli", "2.0.2") -install_package_version("sessioninfo", "1.1.1") -install_package_version("rlang", "0.4.7") -install_package_version("ellipsis", "0.3.1") -install_package_version("xml2", "1.3.2") -install_package_version("curl", "4.3") -install_package_version("rversions", "2.0.2") -install_package_version("remotes", "2.2.0") -install_package_version("R6", "2.4.1") -install_package_version("praise", "1.0.0") -install_package_version("magrittr", "1.5") -install_package_version("digest", "0.6.25") -install_package_version("evaluate", "0.14") -install_package_version("backports", "1.1.8") -install_package_version("rprojroot", "1.3-2") -install_package_version("desc", "1.2.0") -install_package_version("prettyunits", "1.1.1") -install_package_version("ps", "1.3.3") -install_package_version("processx", "3.4.3") -install_package_version("callr", "3.4.3") -install_package_version("pkgbuild", "1.1.0") -install_package_version("rstudioapi", "0.11") -install_package_version("pkgload", "1.1.0") -install_package_version("testthat", "2.3.2") -install_package_version("mime", "0.9") -install_package_version("sys", "3.4") -install_package_version("askpass", "1.1") -install_package_version("openssl", "1.4.2") -install_package_version("httr", "1.4.2") -install_package_version("fs", "1.4.2") -install_package_version("git2r", "0.27.1") -install_package_version("whisker", "0.4") -install_package_version("clipr", "0.7.0") -install_package_version("ini", "0.3.1") -install_package_version("gh", "1.1.0") -install_package_version("utf8", "1.1.4") -install_package_version("lifecycle", "0.2.0") -install_package_version("vctrs", "0.3.2") -install_package_version("pillar", "1.4.6") -install_package_version("pkgconfig", "2.0.3") -install_package_version("tibble", "3.0.3") -install_package_version("rematch2", "2.1.2") -install_package_version("purrr", "0.3.4") -install_package_version("yaml", "2.2.1") -install_package_version("usethis", "1.6.1") -install_package_version("lazyeval", "0.2.2") -install_package_version("rex", "1.2.0") -install_package_version("covr", "3.5.0") -install_package_version("memoise", "1.1.0") -install_package_version("base64enc", "0.1-3") -install_package_version("htmltools", "0.5.0") -install_package_version("crosstalk", "1.1.0.1") -install_package_version("htmlwidgets", "1.5.1") -install_package_version("Rcpp", "1.0.5") -install_package_version("BH", "1.72.0-3") -install_package_version("later", "1.1.0.1") -install_package_version("promises", "1.1.1") -install_package_version("DT", "0.14") -install_package_version("commonmark", "1.7") -install_package_version("stringi", "1.4.6") -install_package_version("stringr", "1.4.0") -install_package_version("xfun", "0.16") -install_package_version("markdown", "1.1") -install_package_version("highr", "0.8") -install_package_version("knitr", "1.29") -install_package_version("brew", "1.0-6") -install_package_version("roxygen2", "7.1.1") -install_package_version("xopen", "1.0.0") -install_package_version("rcmdcheck", "1.3.3") -install_package_version("devtools", "2.3.1") diff --git a/common/dockerfiles/Dockerfile.base b/common/dockerfiles/Dockerfile.base index 5b833cef9..13987e894 100644 --- a/common/dockerfiles/Dockerfile.base +++ b/common/dockerfiles/Dockerfile.base @@ -12,7 +12,6 @@ ENV DEBIAN_FRONTEND=noninteractive # For whatever reason this worked and 'en_US.UTF-8' did not. ENV LANG=C.UTF-8 -COPY workers/CRAN.gpg . RUN apt-get update && \ apt-get install --no-install-recommends -y software-properties-common && \ add-apt-repository ppa:apt-fast/stable && \ @@ -23,43 +22,48 @@ RUN apt-get update && \ apt-fast \ apt-transport-https \ gpg-agent && \ - apt-key add CRAN.gpg && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ apt-fast update -qq && apt-fast install -y \ build-essential \ cmake \ curl \ + cython \ cython3 \ ed \ + file \ git \ libcairo-dev \ libcurl4-gnutls-dev \ libedit-dev \ + libfreetype6-dev \ + libfribidi-dev \ libgit2-dev \ + libharfbuzz-dev \ + libjpeg-dev \ + libpng-dev \ libpq-dev \ libssl-dev \ + libtiff5-dev \ libxml2-dev \ llvm-10-dev \ lsb-release \ mercurial \ pkg-config \ python3-pip \ + python3-pybind11 \ python3.8 \ python3.8-dev \ r-base-core \ wget && \ apt-get clean && \ - rm CRAN.gpg && \ rm -rf /var/lib/apt/lists/* && \ ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ update-alternatives --install \ /usr/bin/python3 python3 /usr/bin/python3.8 1 && \ + pip3 install --upgrade pip setuptools && \ groupadd user && \ useradd --create-home --home-dir /home/user/ -g user user && \ chown -R user /home/user/ -# Pre-install dev tools. ENV R_LIBS=/usr/local/lib/R/site-library -COPY common/R/install_devtools.R . -RUN Rscript install_devtools.R + +ENTRYPOINT [] diff --git a/workers/CRAN.gpg b/workers/CRAN.gpg deleted file mode 100644 index e77c79f27..000000000 --- a/workers/CRAN.gpg +++ /dev/null @@ -1,44 +0,0 @@ ------BEGIN PGP PUBLIC KEY BLOCK----- - -mQENBEy9tcUBCACnWQfqdrcz7tQL/iCeWDYSYPwXpPMUMLE721HfFH7d8ErunPKP -Iwq1v4CrNmMjcainofbu/BfuZESSK1hBAItOk/5VTkzCJlzkrHY9g5v+XlBMPDQC -9u4AE/myw3p52+0NXsnBz+a35mxJKMl+9v9ztvueA6EmLr2xaLf/nx4XwXUMSi1L -p8i8XpAOz/Xg1fspPMRhuDAGYDnOh4uH1jADGoqYaPMty0yVEmzx74qvdIOvfgj1 -6A/9LYXk67td6/JQ5LFCZmFsbahAsqi9inNgBZmnfXO4m4lhzeqNjJAgaw7Fz2zq -UmvpEheKKClgTQMWWNI9Rx1L8IKnJkuKnpzHABEBAAG0I01pY2hhZWwgUnV0dGVy -IDxtYXJ1dHRlckBnbWFpbC5jb20+iQFVBBMBCgA/AhsjBgsJCAcDAgYVCAIJCgsE -FgIDAQIeAQIXgBYhBOKYo6glwNZd/VfLtlFxZhnghNq5BQJfdcXRBQkf4HiMAAoJ -EFFxZhnghNq5Nj8IAIqCTIJ/tMjZqiuwe7M4DQjpvGn4/QylkYLxn9dOds2nGz82 -FAukEAR3tmi22TETrqm+UOukFYUnAEOez7BJiWPwCDt9WuD4/Be8rzbjL0wEv/xu -D1JHWxVd2DfbJG1PAsuv7ouFYbIdh24a8gYpasd6twdbPOdZa0mbzVOnwAmHiH1l -gv2oY/McOO7unc/YGuD2lTb5ohK8yYdHsL5WYK7REPGqEGBS+ppUPWjI1qQb/g2X -oSVfbLLiXiwdvBS4hzjiwGNe76B2jDuHsfmQFMdVpOzNKN0dk10Nm8MFFPrirUIl -Uwk45UttOrcrs5hluLYZhMYHPJ/UNYnaJeJH58iJAT4EEwECACgCGyMGCwkIBwMC -BhUIAgkKCwQWAgMBAh4BAheABQJWI9aNBQkSzCJAAAoJEFFxZhnghNq5/UEH/39q -0IDpE52iqcu7fFWy0ZuENfTeAkV/T8HQ4+CsnZLhih8+DjEDDsde2DKBj/69f8NJ -Q0VOW5LcfSgvgFLBKy+8i1Pp+1rTiBsSU/0kwW/Gky+WKCEqmDLcP0AaMjnCLw9J -cmf3V2xbVeZCPs6iHo6bpNBt31b5YcM8dyY2Ee8yCW4K8LVlP8ndFJtKVkhs2VCz -IAM71McWuQ3IHZxl7wdbpra/I/sDIwaWZd8p0cNBMbVq0PN3/SYAhIqBjgJSBPVQ -Ym7EbfY3Z4/Bxz+BahEdAbkwP7Gbqh6eGGA51T5/RNyssf2fofgGADg9NlHz9pqN -7vx2IlJRrN/pxFobR4OJAT4EEwECACgFAky9tcUCGyMFCQlmAYAGCwkIBwMCBhUI -AgkKCwQWAgMBAh4BAheAAAoJEFFxZhnghNq5PL0H/iGY9DsJKrDk5Lm3xTnLfwGW -od+WoV6/eWVfrywO3Nh/FC5BVLd/E4GgaYVD8tJ9dBiEMboFlricO4NkSBvzUy+P -QMkOXiWbIx0Ggi9eG90Q8N3+us4b7W3b5Ljb1b00g5sppkKz1pu/Z+ciGu3IBbFz -+I1HmsmY1ejO8lUYCAB42EFKu9moVo8n/8GSVsmF9WA3C3gaC0WXW9pTssxoys5Y -D0fJ3x1YAa4iFthc44WPVZn86FSVheewh7xfNonXLovJWQIuFon4xW0bBNesVdGE -EtCWEJgF87bmL88ZRZK9GEjyf+v5Ew067LUeUVt7FFiu09c4tuI7wCUW4ixf2dG5 -AQ0ETL21xQEIANhwKBAIIXVZRKftz9Vc2+CZfO+/eVVf1aRXhB2lwleK1eCVd1Nn -BXEa/cDEO5osTDFbutuopE/NY2OPjFMfG3Zi1SMT6YtXnwAVpBBTdqV1gbwk+vZ6 -iEw1mqnYEbah1zPDl5o7mXkROMLWcdSMNSby6zdNXcBc5qMCOC4a+kbcMqfH+Ca5 -2swFVIAQTriR5/IGZJHrzfOReHD42BtMgP1dbU5KoT58tB/RnNASiMx19gh5XxDm -wwCZTcn0B2WnIOtlphGaKmGDDw9lMxqGHP+joHPunpWoDg1yavbbmee0iyNRTPSI -rpOxo6L0qsXbdHD4gNQl5iR1VvAapq+AkbUAEQEAAYkBPAQYAQoAJgIbDBYhBOKY -o6glwNZd/VfLtlFxZhnghNq5BQJfdcYKBQkf4HjFAAoJEFFxZhnghNq5J2MH/13z -MlB45Qire0mklv3z/Q0HVHTgV8QTRWNdb2GTOQAWQjJZrupkz/MlwmFejoSJo1uM -/Dhb19PNnLyVQ7onHBd7+aeKJ+Tbzlw2dHkgjO3bkRVFVBg0U2xg5a7NXlqSoaAz -Wi+py6bQR+Qj1AIbZNL9s24LSUfazPc2hO7TsNQoOUCGBQ8zvot2oRXOe/4lOmja -YFTb13KXr5ozrKzkm66p07QM5lXmrdsgterKUDdf00+zEXG+vo+j5qnz77Tghahi -vkZip3FwW5APP+/8fWrQRSMzBToFWGZuLri4uef+P/7D2jiqon7LxHKcTkL+MajT -gwrtuH5bI7hL2445uew= -=TIsN ------END PGP PUBLIC KEY BLOCK----- From 5cd9d96d5a2e3c457e9f484e1fbb1c0b8d5e3a37 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 3 Mar 2023 11:09:12 -0800 Subject: [PATCH 073/220] Update common requirements. --- common/requirements.in | 6 +- common/requirements.txt | 148 +++++++++++++--------------------------- 2 files changed, 50 insertions(+), 104 deletions(-) diff --git a/common/requirements.in b/common/requirements.in index a5d3e724a..0002337e5 100644 --- a/common/requirements.in +++ b/common/requirements.in @@ -1,5 +1,5 @@ coverage -django>=3.2.5 +django>=3.2,<4 psycopg2-binary>=2.8.4 boto3>=1.12.21 requests>=2.23.0 @@ -11,8 +11,10 @@ elasticsearch-dsl<7.0.0 django-elasticsearch-dsl-drf # Can't go above 6.X without upgrading elasticsearch django-elasticsearch-dsl==6.5.0 -djangorestframework>=3.11.2 +djangorestframework<3.14.0 vcrpy django-computedfields>=0.1.5 pyyaml>=5.4 sqlparse>=0.4.2 +markupsafe==2.0.0 +rpy2==3.4.5 diff --git a/common/requirements.txt b/common/requirements.txt index b8efe2dea..d5b0e70fe 100644 --- a/common/requirements.txt +++ b/common/requirements.txt @@ -1,106 +1,50 @@ # -# This file is autogenerated by pip-compile +# This file is autogenerated by pip-compile with python 3.9 # To update, run: # -# pip-compile requirements.in +# pip-compile --annotation-style=line common/requirements.in # -asgiref==3.3.4 - # via django -boto3==1.17.93 - # via -r requirements.in -botocore==1.20.93 - # via - # boto3 - # s3transfer -certifi==2021.5.30 - # via requests -chardet==4.0.0 - # via requests -coverage==5.5 - # via -r requirements.in -daiquiri==3.0.0 - # via -r requirements.in -django==3.2.15 - # via - # -r requirements.in - # django-nine - # djangorestframework -django-computedfields==0.1.5 - # via -r requirements.in -django-elasticsearch-dsl==6.5.0 - # via - # -r requirements.in - # django-elasticsearch-dsl-drf -django-elasticsearch-dsl-drf==0.22.1 - # via -r requirements.in -django-nine==0.2.4 - # via django-elasticsearch-dsl-drf -djangorestframework==3.12.4 - # via - # -r requirements.in - # django-elasticsearch-dsl-drf -elasticsearch==6.8.2 - # via - # django-elasticsearch-dsl-drf - # elasticsearch-dsl -elasticsearch-dsl==6.4.0 - # via - # -r requirements.in - # django-elasticsearch-dsl - # django-elasticsearch-dsl-drf -idna==2.10 - # via - # requests - # yarl -jmespath==0.10.0 - # via - # boto3 - # botocore -multidict==5.1.0 - # via yarl -psycopg2-binary==2.8.6 - # via -r requirements.in -python-dateutil==2.8.1 - # via - # botocore - # elasticsearch-dsl -python-json-logger==2.0.1 - # via daiquiri -pytz==2021.1 - # via django -pyyaml==5.4.1 - # via - # -r requirements.in - # vcrpy -raven==6.10.0 - # via -r requirements.in -requests==2.25.1 - # via -r requirements.in -retrying==1.3.3 - # via -r requirements.in -s3transfer==0.4.2 - # via boto3 -six==1.16.0 - # via - # django-elasticsearch-dsl - # django-elasticsearch-dsl-drf - # elasticsearch-dsl - # python-dateutil - # retrying - # vcrpy -sqlparse==0.4.2 - # via - # -r requirements.in - # django -urllib3==1.26.5 - # via - # -r requirements.in - # botocore - # elasticsearch - # requests -vcrpy==4.1.1 - # via -r requirements.in -wrapt==1.12.1 - # via vcrpy -yarl==1.6.3 - # via vcrpy +asgiref==3.6.0 # via django +boto3==1.26.71 # via -r common/requirements.in +botocore==1.29.71 # via boto3, s3transfer +certifi==2022.12.7 # via requests +cffi==1.15.1 # via rpy2 +charset-normalizer==3.0.1 # via requests +coverage==7.1.0 # via -r common/requirements.in +daiquiri==3.2.1 # via -r common/requirements.in +django==3.2.18 # via -r common/requirements.in, django-computedfields, django-fast-update, django-nine, djangorestframework +django-computedfields==0.2.1 # via -r common/requirements.in +django-elasticsearch-dsl==6.5.0 # via -r common/requirements.in, django-elasticsearch-dsl-drf +django-elasticsearch-dsl-drf==0.22.5 # via -r common/requirements.in +django-fast-update==0.2.1 # via django-computedfields +django-nine==0.2.7 # via django-elasticsearch-dsl-drf +djangorestframework==3.13.1 # via -r common/requirements.in, django-elasticsearch-dsl-drf +elasticsearch==6.8.2 # via django-elasticsearch-dsl-drf, elasticsearch-dsl +elasticsearch-dsl==6.4.0 # via -r common/requirements.in, django-elasticsearch-dsl, django-elasticsearch-dsl-drf +idna==3.4 # via requests, yarl +jinja2==3.1.2 # via rpy2 +jmespath==1.0.1 # via boto3, botocore +markupsafe==2.0.0 # via -r common/requirements.in, jinja2 +multidict==6.0.4 # via yarl +packaging==23.0 # via django-nine +psycopg2-binary==2.9.5 # via -r common/requirements.in +pycparser==2.21 # via cffi +python-dateutil==2.8.2 # via botocore, elasticsearch-dsl +python-json-logger==2.0.6 # via daiquiri +pytz==2022.7.1 # via django, djangorestframework, rpy2 +pytz-deprecation-shim==0.1.0.post0 # via tzlocal +pyyaml==6.0 # via -r common/requirements.in, vcrpy +raven==6.10.0 # via -r common/requirements.in +requests==2.28.2 # via -r common/requirements.in +retrying==1.3.4 # via -r common/requirements.in +rpy2==3.4.5 # via -r common/requirements.in +s3transfer==0.6.0 # via boto3 +six==1.16.0 # via django-elasticsearch-dsl, django-elasticsearch-dsl-drf, elasticsearch-dsl, python-dateutil, retrying, vcrpy +sqlparse==0.4.3 # via -r common/requirements.in, django +typing-extensions==4.5.0 # via django-computedfields +tzdata==2022.7 # via pytz-deprecation-shim +tzlocal==4.2 # via rpy2 +urllib3==1.26.14 # via -r common/requirements.in, botocore, elasticsearch, requests +vcrpy==4.2.1 # via -r common/requirements.in +wrapt==1.14.1 # via vcrpy +yarl==1.8.2 # via vcrpy From 3857b0f89608ac6c3ce5f8114b507e103d32d98e Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 3 Mar 2023 11:14:28 -0800 Subject: [PATCH 074/220] Add setup.py. --- common/setup.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/common/setup.py b/common/setup.py index 7bb05f857..64d62624c 100644 --- a/common/setup.py +++ b/common/setup.py @@ -26,7 +26,7 @@ "boto3>=1.9.16", "coverage>=4.5.1", "daiquiri>=1.5.0", - "django>=2.1.8", + "django>=3.2,<4", "raven>=6.9.0", "requests>=2.10.1", "retrying>=1.3.3", From 8ff42fad178f93253e29acc2028ba6de495d8667 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 3 Mar 2023 11:23:44 -0800 Subject: [PATCH 075/220] Update common tests: - Update docker image build instructions. - Fix microarray test data path. - Add platform flag to test run command. --- common/dockerfiles/Dockerfile.common_tests | 76 +++------------------- common/run_tests.sh | 1 + common/tests/test_microarray.py | 8 +-- 3 files changed, 15 insertions(+), 70 deletions(-) diff --git a/common/dockerfiles/Dockerfile.common_tests b/common/dockerfiles/Dockerfile.common_tests index da18704b2..2a31940f4 100644 --- a/common/dockerfiles/Dockerfile.common_tests +++ b/common/dockerfiles/Dockerfile.common_tests @@ -1,79 +1,23 @@ -FROM ubuntu:18.04 +FROM ccdlstaging/dr_base:latest -RUN apt-get update -qq -RUN apt-get install -y software-properties-common -RUN add-apt-repository ppa:apt-fast/stable -RUN apt-get update -qq -RUN apt-get -y install apt-fast +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - -# The packages related to R are somewhat weird, see the README for more details. - -COPY workers/CRAN.gpg . -RUN \ - apt-fast update -qq && \ - apt-get install -y apt-transport-https && \ - apt-fast install -y lsb-release && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ - apt-key add CRAN.gpg && \ - apt-fast update -qq && \ - apt-fast install -y \ - ed \ - git \ - python3 \ - python3-pip \ - python3-dev \ - r-base-core \ - r-base-dev \ - libpq-dev \ - libxml2-dev \ - libssl-dev \ - libcurl4-openssl-dev \ - curl \ - wget && \ - rm -rf /var/lib/apt/lists/* -RUN rm CRAN.gpg - -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -ENV R_LIBS "/usr/local/lib/R/site-library" - -COPY common/install_devtools.R . - -RUN Rscript install_devtools.R - -COPY workers/install_bioc.R . - -RUN Rscript install_bioc.R - -COPY workers/install_affy_only.R . - -RUN Rscript install_affy_only.R - -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG C.UTF-8 - -COPY config config +COPY common/R/dependencies/common_tests/renv.lock . +COPY common/R/renv_load.R . +RUN Rscript renv_load.R COPY common/requirements.txt . - -RUN pip3 install --no-cache-dir -r requirements.txt - -# Install this rpy2 here instead of via requirements.txt because -# pip-compile throws an error for it. -RUN pip3 install rpy2==3.4.5 +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt COPY common/ . +COPY config config ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -ENTRYPOINT [""] +ENTRYPOINT [] diff --git a/common/run_tests.sh b/common/run_tests.sh index 06de9ac44..dfed8defd 100755 --- a/common/run_tests.sh +++ b/common/run_tests.sh @@ -40,5 +40,6 @@ docker run -t $INTERACTIVE \ --add-host=database:"$DB_HOST_IP" \ --add-host=elasticsearch:"$ES_HOST_IP" \ --env-file common/environments/test \ + --platform linux/amd64 \ --volume "$volume_directory":/home/user/data_store \ ccdlstaging/dr_common_tests bash -c "$(run_tests_with_coverage "$@")" --parallel diff --git a/common/tests/test_microarray.py b/common/tests/test_microarray.py index 4bc66d039..32c87b648 100644 --- a/common/tests/test_microarray.py +++ b/common/tests/test_microarray.py @@ -6,15 +6,15 @@ class MicroarrayTestCase(TestCase): def test_get_platform_from_CEL(self): self.assertEqual( - "hgu95av2", microarray.get_platform_from_CEL("test/data/microarray/C30057.CEL.gz") + "hgu95av2", microarray.get_platform_from_CEL("tests/data/microarray/C30057.CEL.gz") ) self.assertEqual( - "rgu34a", microarray.get_platform_from_CEL("test/data/microarray/SG2_u34a.CEL.gz") + "rgu34a", microarray.get_platform_from_CEL("tests/data/microarray/SG2_u34a.CEL.gz") ) self.assertEqual( "mouse4302", - microarray.get_platform_from_CEL("test/data/microarray/97_(Mouse430_2).CEL.gz"), + microarray.get_platform_from_CEL("tests/data/microarray/97_(Mouse430_2).CEL.gz"), ) self.assertEqual( - "zebgene11st", microarray.get_platform_from_CEL("test/data/microarray/CONTROL6.cel.gz") + "zebgene11st", microarray.get_platform_from_CEL("tests/data/microarray/CONTROL6.cel.gz") ) From 2f4c8dfe4d961f5600cf55d856b29d4cca4fbecf Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 3 Mar 2023 11:40:03 -0800 Subject: [PATCH 076/220] Use docker base image for foreman. --- foreman/dockerfiles/Dockerfile.foreman | 26 +------------------------- 1 file changed, 1 insertion(+), 25 deletions(-) diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 043ab3700..f1781ec0a 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -1,32 +1,8 @@ -FROM ubuntu:18.04 +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Source: https://github.com/thisbejim/Pyrebase/issues/87#issuecomment-354452082 -# For whatever reason this worked and 'en_US.UTF-8' did not. -ENV LANG=C.UTF-8 - -# Prevent tzdata from prompting us for a timezone and hanging the build. -ENV DEBIAN_FRONTEND=noninteractive - -RUN apt-get update -qq && \ - apt-get install --no-install-recommends -y software-properties-common && \ - add-apt-repository ppa:apt-fast/stable && \ - apt-get update -qq && \ - apt-get install --no-install-recommends -y apt-fast && \ - apt-fast update -qq && \ - apt-fast install -y \ - gcc \ - libpq-dev \ - python3 \ - python3-pip && \ - apt-get clean && \ - rm -rf /var/lib/apt/lists/* && \ - groupadd user && \ - useradd --create-home --home-dir /home/user -g user user && \ - pip3 install --ignore-installed --upgrade pip - WORKDIR /home/user COPY foreman/requirements.txt . From 26ffbeed2c894fa02faba11739935e87519fa082 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 3 Mar 2023 11:42:07 -0800 Subject: [PATCH 077/220] Update foreman requirements. --- foreman/requirements.in | 19 +++++----- foreman/requirements.txt | 78 ++++++++++++++++++++-------------------- 2 files changed, 49 insertions(+), 48 deletions(-) diff --git a/foreman/requirements.in b/foreman/requirements.in index 5559a7856..20b88a17f 100644 --- a/foreman/requirements.in +++ b/foreman/requirements.in @@ -1,22 +1,23 @@ -django>=3.2.5 -psycopg2-binary>=2.8.4 -requests>=2.20.0 +django>=3.2,<4 +psycopg2-binary +requests retrying coverage -GEOparse>=2.0.3 +geoparse python-dateutil raven pyrefinebio>=0.4.7 tblib -djangorestframework>=3.11.2 +djangorestframework<3.14.0 # Can't go above 6.X without upgrading elasticsearch django-elasticsearch-dsl==6.5.0 django-elasticsearch-dsl-drf>=0.17.1 elasticsearch-dsl>=6.1.0,<7.0.0 vcrpy -django-computedfields>=0.1.5 -pyyaml>=5.4 +django-computedfields +pyyaml # For end-to-end tests -numpy +numpy==1.19.5 +pandas==1.1.5 scipy -sqlparse>=0.4.2 +sqlparse diff --git a/foreman/requirements.txt b/foreman/requirements.txt index b14125bff..bd5406014 100644 --- a/foreman/requirements.txt +++ b/foreman/requirements.txt @@ -1,46 +1,46 @@ # -# This file is autogenerated by pip-compile +# This file is autogenerated by pip-compile with python 3.9 # To update, run: # -# pip-compile requirements.in +# pip-compile --annotation-style=line foreman/requirements.in # -asgiref==3.3.4 # via django -certifi==2021.5.30 # via requests -chardet==4.0.0 # via requests -click==8.0.1 # via pyrefinebio -coverage==5.5 # via -r requirements.in -django-computedfields==0.1.5 # via -r requirements.in -django-elasticsearch-dsl-drf==0.22.1 # via -r requirements.in -django-elasticsearch-dsl==6.5.0 # via -r requirements.in, django-elasticsearch-dsl-drf -django-nine==0.2.4 # via django-elasticsearch-dsl-drf -django==3.2.7 # via -r requirements.in, django-nine, djangorestframework -djangorestframework==3.12.4 # via -r requirements.in, django-elasticsearch-dsl-drf -elasticsearch-dsl==6.4.0 # via -r requirements.in, django-elasticsearch-dsl, django-elasticsearch-dsl-drf +asgiref==3.6.0 # via django +certifi==2022.12.7 # via requests +charset-normalizer==3.0.1 # via requests +click==8.1.3 # via pyrefinebio +coverage==7.1.0 # via -r foreman/requirements.in +django==3.2.17 # via -r foreman/requirements.in, django-computedfields, django-fast-update, django-nine, djangorestframework +django-computedfields==0.2.1 # via -r foreman/requirements.in +django-elasticsearch-dsl==6.5.0 # via -r foreman/requirements.in, django-elasticsearch-dsl-drf +django-elasticsearch-dsl-drf==0.22.5 # via -r foreman/requirements.in +django-fast-update==0.2.1 # via django-computedfields +django-nine==0.2.7 # via django-elasticsearch-dsl-drf +djangorestframework==3.13.1 # via -r foreman/requirements.in, django-elasticsearch-dsl-drf elasticsearch==6.8.2 # via django-elasticsearch-dsl-drf, elasticsearch-dsl -geoparse==2.0.3 # via -r requirements.in -idna==2.10 # via requests, yarl -importlib-metadata==4.5.0 # via click -iso8601==0.1.16 # via pyrefinebio -multidict==5.1.0 # via yarl -numpy==1.19.5 # via -r requirements.in, geoparse, pandas, scipy -pandas==1.1.5 # via geoparse -psycopg2-binary==2.8.6 # via -r requirements.in -pyrefinebio==0.4.7 # via -r requirements.in -python-dateutil==2.8.1 # via -r requirements.in, elasticsearch-dsl, pandas +elasticsearch-dsl==6.4.0 # via -r foreman/requirements.in, django-elasticsearch-dsl, django-elasticsearch-dsl-drf +geoparse==2.0.3 # via -r foreman/requirements.in +idna==3.4 # via requests, yarl +iso8601==1.1.0 # via pyrefinebio +multidict==6.0.4 # via yarl +numpy==1.19.5 # via -r foreman/requirements.in, geoparse, pandas, scipy +packaging==23.0 # via django-nine +pandas==1.1.5 # via -r foreman/requirements.in, geoparse +psycopg2-binary==2.9.5 # via -r foreman/requirements.in +pyrefinebio==0.4.9 # via -r foreman/requirements.in +python-dateutil==2.8.2 # via -r foreman/requirements.in, elasticsearch-dsl, pandas pytimeparse==1.1.8 # via pyrefinebio -pytz==2021.1 # via django, pandas -pyyaml==5.4.1 # via -r requirements.in, pyrefinebio, vcrpy -raven==6.10.0 # via -r requirements.in -requests==2.25.1 # via -r requirements.in, geoparse, pyrefinebio -retrying==1.3.3 # via -r requirements.in -scipy==1.5.4 # via -r requirements.in +pytz==2022.7.1 # via django, djangorestframework, pandas +pyyaml==6.0 # via -r foreman/requirements.in, pyrefinebio, vcrpy +raven==6.10.0 # via -r foreman/requirements.in +requests==2.28.2 # via -r foreman/requirements.in, geoparse, pyrefinebio +retrying==1.3.4 # via -r foreman/requirements.in +scipy==1.10.0 # via -r foreman/requirements.in six==1.16.0 # via django-elasticsearch-dsl, django-elasticsearch-dsl-drf, elasticsearch-dsl, python-dateutil, retrying, vcrpy -sqlparse==0.4.2 # via -r requirements.in, django -tblib==1.7.0 # via -r requirements.in -tqdm==4.61.1 # via geoparse -typing-extensions==3.10.0.0 # via asgiref, importlib-metadata, yarl -urllib3==1.26.5 # via elasticsearch, requests -vcrpy==4.1.1 # via -r requirements.in -wrapt==1.12.1 # via vcrpy -yarl==1.6.3 # via vcrpy -zipp==3.4.1 # via importlib-metadata +sqlparse==0.4.3 # via -r foreman/requirements.in, django +tblib==1.7.0 # via -r foreman/requirements.in +tqdm==4.64.1 # via geoparse +typing-extensions==4.4.0 # via django-computedfields +urllib3==1.26.14 # via elasticsearch, requests +vcrpy==4.2.1 # via -r foreman/requirements.in +wrapt==1.14.1 # via vcrpy +yarl==1.8.2 # via vcrpy From e0c5d8d088fc916bfae391ab6bf8c54b0eefbb2c Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 6 Mar 2023 07:52:43 -0800 Subject: [PATCH 078/220] Fix downloaders tests: - Update requirements. - Fix aspera downloader. - Migrate to new sra data source. - Update docker image. --- .gitignore | 1 + workers/R/dependencies/downloaders/renv.R | 11 + workers/R/dependencies/downloaders/renv.lock | 1787 +++++++++++++++++ .../data_refinery_workers/downloaders/geo.py | 4 +- .../downloaders/requirements.in | 3 +- .../downloaders/requirements.txt | 92 +- .../data_refinery_workers/downloaders/sra.py | 45 +- workers/dockerfiles/Dockerfile.downloaders | 35 +- workers/keys/asperaweb_id_dsa.openssh | 12 + workers/tests/downloaders/test_imports.py | 10 +- workers/tests/downloaders/test_sra.py | 9 +- 11 files changed, 1893 insertions(+), 116 deletions(-) create mode 100644 workers/R/dependencies/downloaders/renv.R create mode 100644 workers/R/dependencies/downloaders/renv.lock create mode 100644 workers/keys/asperaweb_id_dsa.openssh diff --git a/.gitignore b/.gitignore index 1a543b4b8..4de36889a 100644 --- a/.gitignore +++ b/.gitignore @@ -8,6 +8,7 @@ test_volume/* workers/test_volume/processed/TEST/TRANSCRIPTOME_INDEX/Homo_sapiens_short.tar.gz volumes_postgres/ aspera* +!workers/keys/asperaweb_id_dsa.openssh common/version # Project-specific Batch related files diff --git a/workers/R/dependencies/downloaders/renv.R b/workers/R/dependencies/downloaders/renv.R new file mode 100644 index 000000000..de5da9813 --- /dev/null +++ b/workers/R/dependencies/downloaders/renv.R @@ -0,0 +1,11 @@ +renv::install( + "affyio", + "dplyr@1.0.2", + "ff@2.2-14", + "httr@1.4.4", + "oligo", + "oligoClasses", + "purrr@0.3.5", + "readr@1.3.1", + "zlibbioc" +) diff --git a/workers/R/dependencies/downloaders/renv.lock b/workers/R/dependencies/downloaders/renv.lock new file mode 100644 index 000000000..7bdd92f8b --- /dev/null +++ b/workers/R/dependencies/downloaders/renv.lock @@ -0,0 +1,1787 @@ +{ + "R": { + "Version": "3.4.4", + "Repositories": [ + { + "Name": "Bioconductor", + "URL": "https://bioconductor.org/packages/3.6/bioc" + }, + { + "Name": "BioconductorAnnotation", + "URL": "https://bioconductor.org/packages/3.6/data/annotation" + }, + { + "Name": "BioconductorExperiment", + "URL": "https://bioconductor.org/packages/3.6/data/experiment" + }, + { + "Name": "CRAN", + "URL": "https://cloud.r-project.org" + } + ] + }, + "Packages": { + "AnnotationDbi": { + "Package": "AnnotationDbi", + "Version": "1.40.0", + "Source": "Bioconductor", + "Hash": "abb20d74ae5ea08a8d9616a6f1bbaca5", + "Requirements": [ + "Biobase", + "BiocGenerics", + "DBI", + "IRanges", + "RSQLite", + "S4Vectors" + ] + }, + "BH": { + "Package": "BH", + "Version": "1.81.0-1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "68122010f01c4dcfbe58ce7112f2433d", + "Requirements": [] + }, + "Biobase": { + "Package": "Biobase", + "Version": "2.38.0", + "Source": "Bioconductor", + "Hash": "8c8060a3f339c35f1dc14af3b7d22d71", + "Requirements": [ + "BiocGenerics" + ] + }, + "BiocGenerics": { + "Package": "BiocGenerics", + "Version": "0.24.0", + "Source": "Bioconductor", + "Hash": "108ff3e3233e79aef825decc9fa87ee3", + "Requirements": [] + }, + "BiocInstaller": { + "Package": "BiocInstaller", + "Version": "1.28.0", + "Source": "Bioconductor", + "Hash": "ee02868b8bd3657a3463d8bc3a64d831", + "Requirements": [] + }, + "BiocManager": { + "Package": "BiocManager", + "Version": "1.30.20", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "a7fca16a50b6ef7771b49d636dd54b57", + "Requirements": [] + }, + "Biostrings": { + "Package": "Biostrings", + "Version": "2.46.0", + "Source": "Bioconductor", + "Hash": "12005b8dd90f7f7f9864ee9d6965c366", + "Requirements": [ + "BiocGenerics", + "IRanges", + "S4Vectors", + "XVector" + ] + }, + "DBI": { + "Package": "DBI", + "Version": "1.1.3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "b2866e62bab9378c3cc9476a1954226b", + "Requirements": [] + }, + "DelayedArray": { + "Package": "DelayedArray", + "Version": "0.4.1", + "Source": "Bioconductor", + "Hash": "c2572a27fb1281ee4c11390826f326a1", + "Requirements": [ + "BiocGenerics", + "IRanges", + "S4Vectors", + "matrixStats" + ] + }, + "GenomeInfoDb": { + "Package": "GenomeInfoDb", + "Version": "1.14.0", + "Source": "Bioconductor", + "Hash": "39dc4261100e402165a6db62f364b493", + "Requirements": [ + "BiocGenerics", + "GenomeInfoDbData", + "IRanges", + "RCurl", + "S4Vectors" + ] + }, + "GenomeInfoDbData": { + "Package": "GenomeInfoDbData", + "Version": "1.0.0", + "Source": "Bioconductor", + "Hash": "336cc20fd69db19ebfad8a73e05d88b3", + "Requirements": [] + }, + "GenomicRanges": { + "Package": "GenomicRanges", + "Version": "1.30.3", + "Source": "Bioconductor", + "Hash": "ecfab21e609cea7ee297e0e1230b5bb4", + "Requirements": [ + "BiocGenerics", + "GenomeInfoDb", + "IRanges", + "S4Vectors", + "XVector" + ] + }, + "IRanges": { + "Package": "IRanges", + "Version": "2.12.0", + "Source": "Bioconductor", + "Hash": "d89aecf720b113b47f936c48b356d264", + "Requirements": [ + "BiocGenerics", + "S4Vectors" + ] + }, + "KernSmooth": { + "Package": "KernSmooth", + "Version": "2.23-15", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "081f417f4d6d55b7e8981433e8404a22", + "Requirements": [] + }, + "MASS": { + "Package": 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"Package": "Rcpp", + "Version": "1.0.10", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "e749cae40fa9ef469b6050959517453c", + "Requirements": [] + }, + "S4Vectors": { + "Package": "S4Vectors", + "Version": "0.16.0", + "Source": "Bioconductor", + "Hash": "e9cd4cea8c93cbdd8595e562c8ed0624", + "Requirements": [ + "BiocGenerics" + ] + }, + "SummarizedExperiment": { + "Package": "SummarizedExperiment", + "Version": "1.8.1", + "Source": "Bioconductor", + "Hash": "57080b28b2f5606100e02a7ab01b3022", + "Requirements": [ + "Biobase", + "BiocGenerics", + "DelayedArray", + "GenomeInfoDb", + "GenomicRanges", + "IRanges", + "Matrix", + "S4Vectors" + ] + }, + "XVector": { + "Package": "XVector", + "Version": "0.18.0", + "Source": "Bioconductor", + "Hash": "c677dd00d99bd1f81caf0fc3d9fa7672", + "Requirements": [ + "BiocGenerics", + "IRanges", + "S4Vectors", + "zlibbioc" + ] + }, + "affxparser": { + "Package": "affxparser", + "Version": "1.50.0", + "Source": "Bioconductor", + "Hash": 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"Repository", + "Repository": "CRAN", + "Hash": "af8ab99cd936773a148963905736907b", + "Requirements": [ + "BH", + "R6", + "Rcpp", + "clipr", + "crayon", + "hms", + "tibble" + ] + }, + "rematch2": { + "Package": "rematch2", + "Version": "2.1.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "76c9e04c712a05848ae7a23d2f170a40", + "Requirements": [ + "tibble" + ] + }, + "remotes": { + "Package": "remotes", + "Version": "2.4.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "227045be9aee47e6dda9bb38ac870d67", + "Requirements": [] + }, + "renv": { + "Package": "renv", + "Version": "0.16.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "c9e8442ab69bc21c9697ecf856c1e6c7", + "Requirements": [] + }, + "reshape2": { + "Package": "reshape2", + "Version": "1.4.4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "bb5996d0bd962d214a11140d77589917", + "Requirements": [ + "Rcpp", + "plyr", + "stringr" + ] + }, + "rlang": { + "Package": "rlang", + "Version": "1.0.6", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "4ed1f8336c8d52c3e750adcdc57228a7", + "Requirements": [] + }, + "rmarkdown": { + "Package": "rmarkdown", + "Version": "2.20", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "716fde5382293cc94a71f68c85b78d19", + "Requirements": [ + "bslib", + "evaluate", + "htmltools", + "jquerylib", + "jsonlite", + "knitr", + "stringr", + "tinytex", + "xfun", + "yaml" + ] + }, + "roxygen2": { + "Package": "roxygen2", + "Version": "7.2.3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "7b153c746193b143c14baa072bae4e27", + "Requirements": [ + "R6", + "brew", + "cli", + "commonmark", + "cpp11", + "desc", + "knitr", + "pkgload", + "purrr", + "rlang", + "stringi", + "stringr", + "withr", + "xml2" + ] + }, + "rpart": { + "Package": "rpart", + "Version": "4.1-13", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "6315535da80d5cc6c2e573966d8c8210", + "Requirements": [] + }, + "rprojroot": { + "Package": "rprojroot", + "Version": "2.0.3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "1de7ab598047a87bba48434ba35d497d", + "Requirements": [] + }, + "rstudioapi": { + "Package": "rstudioapi", + "Version": "0.14", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "690bd2acc42a9166ce34845884459320", + "Requirements": [] + }, + "rversions": { + "Package": "rversions", + "Version": "2.1.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "a9881dfed103e83f9de151dc17002cd1", + "Requirements": [ + "curl", + "xml2" + ] + }, + "sass": { + "Package": "sass", + "Version": "0.4.5", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "2bb4371a4c80115518261866eab6ab11", + "Requirements": [ + "R6", + "fs", + "htmltools", + "rappdirs", + "rlang" + ] + }, + "sessioninfo": { + "Package": "sessioninfo", + "Version": "1.2.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "3f9796a8d0a0e8c6eb49a4b029359d1f", + "Requirements": [ + "cli" + ] + }, + "shiny": { + "Package": "shiny", + "Version": "1.7.4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "c2eae3d8c670fa9dfa35a12066f4a1d5", + "Requirements": [ + "R6", + "bslib", + "cachem", + "commonmark", + "crayon", + "ellipsis", + "fastmap", + "fontawesome", + "glue", + "htmltools", + "httpuv", + "jsonlite", + "later", + "lifecycle", + "mime", + "promises", + "rlang", + "sourcetools", + "withr", + "xtable" + ] + }, + "sourcetools": { + "Package": "sourcetools", + "Version": "0.1.7-1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "5f5a7629f956619d519205ec475fe647", + "Requirements": [] + }, + "spatial": { + "Package": "spatial", + "Version": "7.3-11", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "f27a078d834614513608da2c08031130", + "Requirements": [] + }, + "stringi": { + "Package": "stringi", + "Version": "1.7.12", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "ca8bd84263c77310739d2cf64d84d7c9", + "Requirements": [] + }, + "stringr": { + "Package": "stringr", + "Version": "1.5.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "671a4d384ae9d32fc47a14e98bfa3dc8", + "Requirements": [ + "cli", + "glue", + "lifecycle", + "magrittr", + "rlang", + "stringi", + "vctrs" + ] + }, + "survival": { + "Package": "survival", + "Version": "2.41-3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "60b1da51f5e3556fd177170a1aa40b49", + "Requirements": [ + "Matrix" + ] + }, + "sys": { + "Package": "sys", + "Version": "3.4.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "34c16f1ef796057bfa06d3f4ff818a5d", + "Requirements": [] + }, + "systemfonts": { + "Package": "systemfonts", + "Version": "1.0.4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "90b28393209827327de889f49935140a", + "Requirements": [ + "cpp11" + ] + }, + "testthat": { + "Package": "testthat", + "Version": "3.1.6", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "7910146255835c66e9eb272fb215248d", + "Requirements": [ + "R6", + "brio", + "callr", + "cli", + "desc", + "digest", + "ellipsis", + "evaluate", + "jsonlite", + "lifecycle", + "magrittr", + "pkgload", + "praise", + "processx", + "ps", + "rlang", + "waldo", + "withr" + ] + }, + "textshaping": { + "Package": "textshaping", + "Version": "0.3.6", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "1ab6223d3670fac7143202cb6a2d43d5", + "Requirements": [ + "cpp11", + "systemfonts" + ] + }, + "tibble": { + "Package": "tibble", + "Version": "3.1.8", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "56b6934ef0f8c68225949a8672fe1a8f", + "Requirements": [ + "fansi", + "lifecycle", + "magrittr", + "pillar", + "pkgconfig", + "rlang", + "vctrs" + ] + }, + "tidyselect": { + "Package": "tidyselect", + "Version": "1.2.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "79540e5fcd9e0435af547d885f184fd5", + "Requirements": [ + "cli", + "glue", + "lifecycle", + "rlang", + "vctrs", + "withr" + ] + }, + "tinytex": { + "Package": "tinytex", + "Version": "0.44", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "c0f007e2eeed7722ce13d42b84a22e07", + "Requirements": [ + "xfun" + ] + }, + "tximport": { + "Package": "tximport", + "Version": "1.6.0", + "Source": "Bioconductor", + "Hash": "2c824ac20bbb34bba92a9b5b0d600fec", + "Requirements": [] + }, + "urlchecker": { + "Package": "urlchecker", + "Version": "1.0.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "409328b8e1253c8d729a7836fe7f7a16", + "Requirements": [ + "cli", + "curl", + "xml2" + ] + }, + "usethis": { + "Package": "usethis", + "Version": "2.1.6", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "a67a22c201832b12c036cc059f1d137d", + "Requirements": [ + "cli", + "clipr", + "crayon", + "curl", + "desc", + "fs", + "gert", + "gh", + "glue", + "jsonlite", + "lifecycle", + "purrr", + "rappdirs", + "rlang", + "rprojroot", + "rstudioapi", + "whisker", + "withr", + "yaml" + ] + }, + "utf8": { + "Package": "utf8", + "Version": "1.2.3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "1fe17157424bb09c48a8b3b550c753bc", + "Requirements": [] + }, + "vctrs": { + "Package": "vctrs", + "Version": "0.5.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "e4ffa94ceed5f124d429a5a5f0f5b378", + "Requirements": [ + "cli", + "glue", + "lifecycle", + "rlang" + ] + }, + "waldo": { + "Package": "waldo", + "Version": "0.4.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "035fba89d0c86e2113120f93301b98ad", + "Requirements": [ + "cli", + "diffobj", + "fansi", + "glue", + "rematch2", + "rlang", + "tibble" + ] + }, + "whisker": { + "Package": "whisker", + "Version": "0.4.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "c6abfa47a46d281a7d5159d0a8891e88", + "Requirements": [] + }, + "withr": { + "Package": "withr", + "Version": "2.5.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "c0e49a9760983e81e55cdd9be92e7182", + "Requirements": [] + }, + "xfun": { + "Package": "xfun", + "Version": "0.37", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "a6860e1400a8fd1ddb6d9b4230cc34ab", + "Requirements": [] + }, + "xml2": { + "Package": "xml2", + "Version": "1.3.3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "40682ed6a969ea5abfd351eb67833adc", + "Requirements": [] + }, + "xopen": { + "Package": "xopen", + "Version": "1.0.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "6c85f015dee9cc7710ddd20f86881f58", + "Requirements": [ + "processx" + ] + }, + "xtable": { + "Package": "xtable", + "Version": "1.8-4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "b8acdf8af494d9ec19ccb2481a9b11c2", + "Requirements": [] + }, + "yaml": { + "Package": "yaml", + "Version": "2.3.7", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "0d0056cc5383fbc240ccd0cb584bf436", + "Requirements": [] + }, + "zip": { + "Package": "zip", + "Version": "2.2.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "c42bfcec3fa6a0cce17ce1f8bc684f88", + "Requirements": [] + }, + "zlibbioc": { + "Package": "zlibbioc", + "Version": "1.24.0", + "Source": "Bioconductor", + "Hash": "75640bcdaa390770a672332c904642db", + "Requirements": [] + } + } +} diff --git a/workers/data_refinery_workers/downloaders/geo.py b/workers/data_refinery_workers/downloaders/geo.py index d395c5f2c..1fc415b16 100644 --- a/workers/data_refinery_workers/downloaders/geo.py +++ b/workers/data_refinery_workers/downloaders/geo.py @@ -73,8 +73,8 @@ def _download_file_aspera( downloader_job=downloader_job.id, ) - ascp = ".aspera/cli/bin/ascp" - key = ".aspera/cli/etc/asperaweb_id_dsa.openssh" + ascp = "ascp" + key = "keys/asperaweb_id_dsa.openssh" url = download_url user = "anonftp" ftp = "ftp-trace.ncbi.nlm.nih.gov" diff --git a/workers/data_refinery_workers/downloaders/requirements.in b/workers/data_refinery_workers/downloaders/requirements.in index 9728c78a8..a368292d3 100644 --- a/workers/data_refinery_workers/downloaders/requirements.in +++ b/workers/data_refinery_workers/downloaders/requirements.in @@ -1,4 +1,4 @@ -django>=3.2.5 +django>=3.2,<4 requests>=2.23.0 urllib3>=1.24.2 psycopg2-binary>=2.8.4 @@ -9,3 +9,4 @@ vcrpy django-computedfields>=0.1.5 pyyaml>=5.4 sqlparse>=0.4.2 +rpy2==3.4.5 diff --git a/workers/data_refinery_workers/downloaders/requirements.txt b/workers/data_refinery_workers/downloaders/requirements.txt index fe064620f..157a33fce 100644 --- a/workers/data_refinery_workers/downloaders/requirements.txt +++ b/workers/data_refinery_workers/downloaders/requirements.txt @@ -1,62 +1,38 @@ # -# This file is autogenerated by pip-compile +# This file is autogenerated by pip-compile with python 3.9 # To update, run: # -# pip-compile requirements.in +# pip-compile --annotation-style=line workers/data_refinery_workers/downloaders/requirements.in # -asgiref==3.3.4 - # via django -certifi==2021.5.30 - # via requests -chardet==4.0.0 - # via requests -coverage==5.5 - # via coveralls -coveralls==3.1.0 - # via -r requirements.in -django==3.2.15 - # via -r requirements.in -django-computedfields==0.1.5 - # via -r requirements.in -docopt==0.6.2 - # via coveralls -idna==2.10 - # via - # requests - # yarl -multidict==5.1.0 - # via yarl -psutil==5.8.0 - # via -r requirements.in -psycopg2-binary==2.8.6 - # via -r requirements.in -pytz==2021.1 - # via django -pyyaml==5.4.1 - # via - # -r requirements.in - # vcrpy -requests==2.25.1 - # via - # -r requirements.in - # coveralls -retrying==1.3.3 - # via -r requirements.in -six==1.16.0 - # via - # retrying - # vcrpy -sqlparse==0.4.2 - # via - # -r requirements.in - # django -urllib3==1.26.5 - # via - # -r requirements.in - # requests -vcrpy==4.1.1 - # via -r requirements.in -wrapt==1.12.1 - # via vcrpy -yarl==1.6.3 - # via vcrpy +asgiref==3.6.0 # via django +certifi==2022.12.7 # via requests +cffi==1.15.1 # via rpy2 +charset-normalizer==3.0.1 # via requests +coverage==6.5.0 # via coveralls +coveralls==3.3.1 # via -r workers/data_refinery_workers/downloaders/requirements.in +django==3.2.18 # via -r workers/data_refinery_workers/downloaders/requirements.in, django-computedfields, django-fast-update +django-computedfields==0.2.1 # via -r workers/data_refinery_workers/downloaders/requirements.in +django-fast-update==0.2.1 # via django-computedfields +docopt==0.6.2 # via coveralls +idna==3.4 # via requests, yarl +jinja2==3.1.2 # via rpy2 +markupsafe==2.1.2 # via jinja2 +multidict==6.0.4 # via yarl +psutil==5.9.4 # via -r workers/data_refinery_workers/downloaders/requirements.in +psycopg2-binary==2.9.5 # via -r workers/data_refinery_workers/downloaders/requirements.in +pycparser==2.21 # via cffi +pytz==2022.7.1 # via django, rpy2 +pytz-deprecation-shim==0.1.0.post0 # via tzlocal +pyyaml==6.0 # via -r workers/data_refinery_workers/downloaders/requirements.in, vcrpy +requests==2.28.2 # via -r workers/data_refinery_workers/downloaders/requirements.in, coveralls +retrying==1.3.4 # via -r workers/data_refinery_workers/downloaders/requirements.in +rpy2==3.4.5 # via -r workers/data_refinery_workers/downloaders/requirements.in +six==1.16.0 # via retrying, vcrpy +sqlparse==0.4.3 # via -r workers/data_refinery_workers/downloaders/requirements.in, django +typing-extensions==4.5.0 # via django-computedfields +tzdata==2022.7 # via pytz-deprecation-shim +tzlocal==4.2 # via rpy2 +urllib3==1.26.14 # via -r workers/data_refinery_workers/downloaders/requirements.in, requests +vcrpy==4.2.1 # via -r workers/data_refinery_workers/downloaders/requirements.in +wrapt==1.14.1 # via vcrpy +yarl==1.8.2 # via vcrpy diff --git a/workers/data_refinery_workers/downloaders/sra.py b/workers/data_refinery_workers/downloaders/sra.py index c4a6a367b..c0268dc5a 100644 --- a/workers/data_refinery_workers/downloaders/sra.py +++ b/workers/data_refinery_workers/downloaders/sra.py @@ -35,29 +35,22 @@ def _download_file( if "ftp.sra.ebi.ac.uk" in download_url: # From: ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_1.fastq.gz # To: era-fasp@fasp.sra.ebi.ac.uk:/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_1.fastq.gz - download_url = download_url.replace("ftp", "era-fasp@fasp") - download_url = download_url.replace(".uk/", ".uk:/") + download_url = download_url.replace("ftp", "era-fasp@fasp").replace(".uk/", ".uk:/") original_file.source_url = download_url return _download_file_aspera( - download_url, downloader_job, target_file_path, 0, original_file, source="ENA" + download_url, + downloader_job, + target_file_path, + 0, + original_file, + source="ENA", ) - elif "ncbi.nlm.nih.gov" in download_url: - # Try to convert old-style endpoints into new-style endpoints if possible - try: - if "anonftp" in download_url or "dbtest" in download_url: - accession = download_url.split("/")[-1].split(".sra")[0] - new_url = get_https_sra_download(accession) - if new_url: - download_url = new_url - except Exception: - pass + elif "sra-pub-run-odp.s3.amazonaws.com" in download_url: return _download_file_http(download_url, downloader_job, target_file_path) else: downloader_job.failure_reason = ("Unrecognized URL pattern: {}").format(download_url) return False - return True - def _download_file_http( download_url: str, downloader_job: DownloaderJob, target_file_path: str @@ -105,26 +98,24 @@ def _download_file_aspera( # aspera.sra.ebi.ac.uk users port 33001 for SSH communication # We are also NOT using encryption (-T) to avoid slowdown, # and we are not using any kind of rate limiting. - command_str = ( - ".aspera/cli/bin/ascp -QT -l 300m -P33001" - " -i .aspera/cli/etc/asperaweb_id_dsa.openssh {src} {dest}" - ) + command_str = "ascp -QT -l 300m -P 33001 -i keys/asperaweb_id_dsa.openssh {src} {dest}" formatted_command = command_str.format(src=download_url, dest=target_file_path) completed_command = subprocess.run( - formatted_command.split(), stdout=subprocess.PIPE, stderr=subprocess.PIPE + formatted_command.split(), + stdout=subprocess.PIPE, + stderr=subprocess.PIPE, ) else: # NCBI requires encryption and recommends -k1 resume, as # well as the 450m limit and -Q (play fair). # ex: https://github.com/AlexsLemonade/refinebio/pull/1189#issuecomment-478018580 - command_str = ( - ".aspera/cli/bin/ascp -p -Q -T -k1 -l 450m" - " -i .aspera/cli/etc/asperaweb_id_dsa.openssh {src} {dest}" - ) + command_str = "ascp -p -Q -T -k1 -l 450m -i keys/asperaweb_id_dsa.openssh {src} {dest}" formatted_command = command_str.format(src=download_url, dest=target_file_path) logger.info("Starting NCBI ascp", time=str(timezone.now())) completed_command = subprocess.run( - formatted_command.split(), stdout=subprocess.PIPE, stderr=subprocess.PIPE + formatted_command.split(), + stdout=subprocess.PIPE, + stderr=subprocess.PIPE, ) logger.info("Ending NCBI ascp", time=str(timezone.now())) @@ -252,7 +243,9 @@ def _replace_dotsra_with_fastq_files( original_file.save() read_two_original_file = OriginalFile.objects.get_or_create( - source_url=read_two_url, source_filename=read_two_url.split("/")[-1], has_raw=True + source_url=read_two_url, + source_filename=read_two_url.split("/")[-1], + has_raw=True, )[0] OriginalFileSampleAssociation.objects.get_or_create( original_file=read_two_original_file, sample=sample diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 9b611956d..1b19485e2 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -5,30 +5,22 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -COPY workers/R/dependencies/install_downloader_R_only.R . -RUN Rscript install_downloader_R_only.R +COPY workers/R/dependencies/downloaders/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/downloaders/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir rpy2==3.4.5 && \ - pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt -# Aspera will only install as the current user. -# Even using `su - user &&` doesn't work... -USER user +# Install Aspera. +RUN gpg --keyserver keyserver.ubuntu.com --recv-keys \ + 409B6B1796C275462A1703113804BB82D39DC0E3 \ + 7D2BAF1CF37B13E2069D6956105BD0E739499BDB && \ + curl -sSL https://get.rvm.io | bash -s stable --ruby --gems=aspera-cli -# Install Aspera. We have to install it using Holland Computing Center's conda -# repo because download.asperasoft.com now returns 403s -RUN wget -q "https://anaconda.org/HCC/aspera-cli/3.9.1/download/\ -linux-64/aspera-cli-3.9.1-0.tar.bz2" && \ - [ "$(sha256sum aspera-cli-3.9.1-0.tar.bz2 | cut -d ' ' -f1)" =\ - "60a09a7f3795186954079869106aa89a64183b7be8e0da7cbbe9d57c66c9bcdb" ] && \ - rm -rf .aspera && \ - mkdir -p .aspera/cli && \ - tar xf aspera-cli-3.9.1-0.tar.bz2 -C .aspera/cli && \ - rm aspera-cli-3.9.1-0.tar.bz2 - -# Now that we're done installing Aspera go back to being root for a bit. +USER user +RUN source /usr/local/rvm/scripts/rvm && \ + ascli conf ascp install USER root # Get the latest version from the dist directory. @@ -40,7 +32,10 @@ COPY .boto .boto COPY config config COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION +ENV PATH="$PATH:/home/user/.aspera/ascli/sdk" ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/keys/asperaweb_id_dsa.openssh b/workers/keys/asperaweb_id_dsa.openssh new file mode 100644 index 000000000..c6c2a528f --- /dev/null +++ b/workers/keys/asperaweb_id_dsa.openssh @@ -0,0 +1,12 @@ +-----BEGIN DSA PRIVATE KEY----- +MIIBuwIBAAKBgQDkKQHD6m4yIxgjsey6Pny46acZXERsJHy54p/BqXIyYkVOAkEp +KgvT3qTTNmykWWw4ovOP1+Di1c/2FpYcllcTphkWcS8lA7j012mUEecXavXjPPG0 +i3t5vtB8xLy33kQ3e9v9/Lwh0xcRfua0d5UfFwopBIAXvJAr3B6raps8+QIVALws +yeqsx3EolCaCVXJf+61ceJppAoGAPoPtEP4yzHG2XtcxCfXab4u9zE6wPz4ePJt0 +UTn3fUvnQmJT7i0KVCRr3g2H2OZMWF12y0jUq8QBuZ2so3CHee7W1VmAdbN7Fxc+ +cyV9nE6zURqAaPyt2bE+rgM1pP6LQUYxgD3xKdv1ZG+kDIDEf6U3onjcKbmA6ckx +T6GavoACgYEAobapDv5p2foH+cG5K07sIFD9r0RD7uKJnlqjYAXzFc8U76wXKgu6 +WXup2ac0Co+RnZp7Hsa9G+E+iJ6poI9pOR08XTdPly4yDULNST4PwlfrbSFT9FVh +zkWfpOvAUc8fkQAhZqv/PE6VhFQ8w03Z8GpqXx7b3NvBR+EfIx368KoCFEyfl0vH +Ta7g6mGwIMXrdTQQ8fZs +-----END DSA PRIVATE KEY----- diff --git a/workers/tests/downloaders/test_imports.py b/workers/tests/downloaders/test_imports.py index 005405f7a..2b2195574 100644 --- a/workers/tests/downloaders/test_imports.py +++ b/workers/tests/downloaders/test_imports.py @@ -4,8 +4,8 @@ class ImportTestCase(TestCase): @tag("downloaders") def test_downloader_imports(self): - # Make sure we can import the downloader tests - import data_refinery_workers.downloaders.test_array_express - import data_refinery_workers.downloaders.test_geo - import data_refinery_workers.downloaders.test_sra - import data_refinery_workers.downloaders.test_transcriptome_index + # Make sure we can import the downloader tests. + from tests.downloaders import test_array_express + from tests.downloaders import test_geo + from tests.downloaders import test_sra + from tests.downloaders import test_transcriptome_index diff --git a/workers/tests/downloaders/test_sra.py b/workers/tests/downloaders/test_sra.py index d07df4454..8c969043c 100644 --- a/workers/tests/downloaders/test_sra.py +++ b/workers/tests/downloaders/test_sra.py @@ -63,8 +63,9 @@ def test_download_file_ncbi(self): dlj.accession_code = "SRR9117853" dlj.save() og = OriginalFile() - og.source_filename = "SRR9117853.sra" - og.source_url = "anonftp@ftp.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/SRR/SRR9117/SRR9117853/SRR9117853.sra" + og.source_filename = "SRR9117853" + og.source_url = "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR9117853/SRR9117853" + og.is_archive = True og.save() sample = Sample() @@ -92,8 +93,8 @@ def test_download_file_swapper(self): dlj.accession_code = "SRR9117853" dlj.save() og = OriginalFile() - og.source_filename = "SRR9117853.sra" - og.source_url = "anonftp@ftp.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/SRR/SRR9117/SRR9117853/SRR9117853.sra" + og.source_filename = "SRR9117853" + og.source_url = "https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR9117853/SRR9117853" og.is_archive = True og.save() sample = Sample() From 632c86f2f2b3c495bf05c26684f014901fb04b1f Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 6 Mar 2023 08:18:54 -0800 Subject: [PATCH 079/220] Add `renv` scripts. Set platform for workers run_tests.sh. --- .../R/dependencies/install_devtools_renv.R | 76 +++++++++++++++++++ workers/R/renv_load.R | 16 ++++ workers/R/renv_save.R | 15 ++++ 3 files changed, 107 insertions(+) create mode 100644 workers/R/dependencies/install_devtools_renv.R create mode 100644 workers/R/renv_load.R create mode 100644 workers/R/renv_save.R diff --git a/workers/R/dependencies/install_devtools_renv.R b/workers/R/dependencies/install_devtools_renv.R new file mode 100644 index 000000000..58c97546b --- /dev/null +++ b/workers/R/dependencies/install_devtools_renv.R @@ -0,0 +1,76 @@ +renv::install("jsonlite@1.7.0") +renv::install("withr@2.2.0") +renv::install("crayon@1.3.4") +renv::install("fansi@0.4.1") +renv::install("glue@1.4.1") +renv::install("assertthat@0.2.1") +renv::install("cli@2.0.2") +renv::install("sessioninfo@1.1.1") +renv::install("rlang@0.4.7") +renv::install("ellipsis@0.3.2") +renv::install("xml2@1.3.2") +renv::install("curl@4.3") +renv::install("rversions@2.0.2") +renv::install("remotes@2.2.0") +renv::install("R6@2.4.1") +renv::install("praise@1.0.0") +renv::install("magrittr@1.5") +renv::install("digest@0.6.25") +renv::install("evaluate@0.14") +renv::install("backports@1.1.8") +renv::install("rprojroot@1.3-2") +renv::install("desc@1.2.0") +renv::install("prettyunits@1.1.1") +renv::install("ps@1.3.3") +renv::install("processx@3.4.3") +renv::install("callr@3.4.3") +renv::install("pkgbuild@1.1.0") +renv::install("rstudioapi@0.11") +renv::install("pkgload@1.1.0") +renv::install("testthat@2.3.2") +renv::install("mime@0.9") +renv::install("sys@3.4") +renv::install("askpass@1.1") +renv::install("openssl@2.0.3") +renv::install("httr@1.4.2") +renv::install("fs@1.4.2") +renv::install("git2r@0.27.1") +renv::install("whisker@0.4") +renv::install("clipr@0.7.0") +renv::install("ini@0.3.1") +renv::install("gh@1.1.0") +renv::install("utf8@1.1.4") +renv::install("lifecycle@1.0.3") +renv::install("vctrs@0.4.1") +renv::install("pillar@1.4.6") +renv::install("pkgconfig@2.0.3") +renv::install("tibble@3.0.3") +renv::install("rematch2@2.1.2") +renv::install("purrr@0.3.4") +renv::install("yaml@2.2.1") +renv::install("usethis@1.6.1") +renv::install("lazyeval@0.2.2") +renv::install("rex@1.2.0") +renv::install("covr@3.5.0") +renv::install("memoise@2.0.1") +renv::install("base64enc@0.1-3") +renv::install("htmltools@0.5.4") +renv::install("crosstalk@1.1.0.1") +renv::install("htmlwidgets@1.5.1") +renv::install("Rcpp@1.0.7") +renv::install("BH@1.72.0-3") +renv::install("later@1.1.0.1") +renv::install("promises@1.1.1") +renv::install("DT@0.14") +renv::install("commonmark@1.7") +renv::install("stringi@1.4.6") +renv::install("stringr@1.4.0") +renv::install("xfun@0.36") +renv::install("markdown@1.1") +renv::install("highr@0.8") +renv::install("knitr@1.29") +renv::install("brew@1.0-6") +renv::install("roxygen2@7.1.1") +renv::install("xopen@1.0.0") +renv::install("rcmdcheck@1.3.3") +renv::install("devtools@2.4.5") diff --git a/workers/R/renv_load.R b/workers/R/renv_load.R new file mode 100644 index 000000000..601e479b1 --- /dev/null +++ b/workers/R/renv_load.R @@ -0,0 +1,16 @@ +options(warn = 2) +options(repos = structure(c( + Bioconductor = "https://bioconductor.org/packages/3.6/bioc", + BioconductorAnnotation = "https://bioconductor.org/packages/3.6/data/annotation", + BioconductorExperiment = "https://bioconductor.org/packages/3.6/data/experiment", + CRAN = "https://cloud.r-project.org" +))) +options(Ncpus = parallel::detectCores()) +options(renv.r.version = "3.4.4") +options(renv.settings.use.cache = FALSE) + +install.packages("BiocInstaller") +install.packages("https://cloud.r-project.org/src/contrib/renv_0.16.0.tar.gz") + +renv::consent(provided = TRUE) +renv::restore(prompt = FALSE, rebuild = TRUE) diff --git a/workers/R/renv_save.R b/workers/R/renv_save.R new file mode 100644 index 000000000..7892c0a3a --- /dev/null +++ b/workers/R/renv_save.R @@ -0,0 +1,15 @@ +options(warn = 2) +options(repos = structure(c( + Bioconductor = "https://bioconductor.org/packages/3.6/bioc", + BioconductorAnnotation = "https://bioconductor.org/packages/3.6/data/annotation", + BioconductorExperiment = "https://bioconductor.org/packages/3.6/data/experiment", + CRAN = "https://cloud.r-project.org" +))) +options(Ncpus = parallel::detectCores()) +options(renv.r.version = "3.4.4") +options(renv.settings.use.cache = FALSE) + +install.packages("BiocInstaller") +install.packages("https://cloud.r-project.org/src/contrib/renv_0.16.0.tar.gz") + +renv::init(bioconductor = "3.6") From 258a3a9a9a52288c6f7fe69f913aaeefcb67d0d8 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 7 Mar 2023 18:05:32 -0800 Subject: [PATCH 080/220] Update smasher dependencies. --- workers/R/dependencies/smasher/renv.R | 21 + workers/R/dependencies/smasher/renv.lock | 1511 ++++++++++++++++++++++ workers/dockerfiles/Dockerfile.smasher | 14 +- 3 files changed, 1538 insertions(+), 8 deletions(-) create mode 100644 workers/R/dependencies/smasher/renv.R create mode 100644 workers/R/dependencies/smasher/renv.lock diff --git a/workers/R/dependencies/smasher/renv.R b/workers/R/dependencies/smasher/renv.R new file mode 100644 index 000000000..9fd953eb3 --- /dev/null +++ b/workers/R/dependencies/smasher/renv.R @@ -0,0 +1,21 @@ +renv::install("codetools@0.2-15") +renv::install("iterators@1.0.10") +renv::install("foreach@1.4.4") +renv::install("doParallel@1.0.11") +renv::install("data.table@1.11.0") +renv::install("getopt@1.20.3") +renv::install("optparse@1.4.4") +renv::install("lazyeval@0.2.1") +renv::install("ff@2.2-14.2") +renv::install("bit@1.1-15.2") +renv::install("dplyr@1.0.2") +renv::install("readr@1.3.1") +renv::install("rlang@1.0.6") +renv::install("ellipsis@0.3.2") + +# Bioconductor. +renv::install( + "oligo", + "oligoClasses", + "preprocessCore" +) diff --git a/workers/R/dependencies/smasher/renv.lock b/workers/R/dependencies/smasher/renv.lock new file mode 100644 index 000000000..5eceeee80 --- /dev/null +++ b/workers/R/dependencies/smasher/renv.lock @@ -0,0 +1,1511 @@ +{ + "R": { + "Version": "3.4.4", + "Repositories": [ + { + "Name": "Bioconductor", + "URL": "https://bioconductor.org/packages/3.6/bioc" + }, + { + "Name": "BioconductorAnnotation", + "URL": "https://bioconductor.org/packages/3.6/data/annotation" + }, + { + "Name": "BioconductorExperiment", + "URL": "https://bioconductor.org/packages/3.6/data/experiment" + }, + { + "Name": "CRAN", + "URL": "https://cloud.r-project.org" + } + ] + }, + "Packages": { + "AnnotationDbi": { + "Package": "AnnotationDbi", + "Version": "1.40.0", + "Source": "Bioconductor", + "Hash": "abb20d74ae5ea08a8d9616a6f1bbaca5", + "Requirements": [ + "Biobase", + "BiocGenerics", + "DBI", + "IRanges", + "RSQLite", + "S4Vectors" + ] + }, + "BH": { + "Package": "BH", + "Version": "1.72.0-3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "8f9ce74c6417d61f0782cbae5fd2b7b0", + "Requirements": [] + }, 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a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index 926f98208..29bba5cd8 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -5,16 +5,12 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -COPY workers/R/dependencies/qn/cran/dependencies.R . -RUN Rscript dependencies.R - -COPY workers/R/dependencies/qn/bioc/dependencies.R . -RUN Rscript dependencies.R +COPY workers/R/dependencies/smasher/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir nose numpy rpy2==3.4.5 && \ - pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ @@ -25,6 +21,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION From 5fc6d3e8df9aace9d49a1ea9da9d06803de52ceb Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 7 Mar 2023 18:13:37 -0800 Subject: [PATCH 081/220] Update transcriptome Docker image. --- workers/dockerfiles/Dockerfile.transcriptome | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index f7dbe3676..3aec1395b 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -6,9 +6,7 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir numpy && \ - pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ # It's annoying that this can only be installed via git. git clone https://github.com/deweylab/RSEM.git && \ cd RSEM && make install && \ @@ -40,6 +38,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION From c007cf27efc0d1284e2b85bee7cb30f978ce94ba Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 9 Mar 2023 09:39:51 -0800 Subject: [PATCH 082/220] Update salmon dependencies. --- workers/R/dependencies/salmon/renv.R | 30 + workers/R/dependencies/salmon/renv.lock | 1834 +++++++++++++++++++++++ workers/dockerfiles/Dockerfile.salmon | 15 +- 3 files changed, 1870 insertions(+), 9 deletions(-) create mode 100644 workers/R/dependencies/salmon/renv.R create mode 100644 workers/R/dependencies/salmon/renv.lock diff --git 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workers/R/dependencies/tximport/cran/dependencies.R tximport_dependencies.R -RUN Rscript tximport_dependencies.R - -# Install tximport. -COPY workers/R/dependencies/tximport/install_tximport.R . -RUN Rscript install_tximport.R +COPY workers/R/dependencies/salmon/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir numpy && \ - pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Install Salmon # Tximport requires all experiments to be processed with the same version of @@ -61,6 +56,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION From 263136ddff2f724696ab44ba8403e17f471960d6 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 9 Mar 2023 09:47:19 -0800 Subject: [PATCH 083/220] Update no_op docker image and R dependencies. --- workers/R/dependencies/no_op/renv.R | 53 + workers/R/dependencies/no_op/renv.lock | 1613 ++++++++++++++++++++++++ workers/dockerfiles/Dockerfile.no_op | 16 +- 3 files changed, 1672 insertions(+), 10 deletions(-) create mode 100644 workers/R/dependencies/no_op/renv.R create mode 100644 workers/R/dependencies/no_op/renv.lock diff --git a/workers/R/dependencies/no_op/renv.R b/workers/R/dependencies/no_op/renv.R new file mode 100644 index 000000000..406954de8 --- /dev/null +++ b/workers/R/dependencies/no_op/renv.R @@ -0,0 +1,53 @@ +renv::install("data.table@1.11.0") +renv::install("getopt@1.20.3") +renv::install("optparse@1.4.4") +renv::install("rlang@0.3.1") +renv::install("crayon@1.3.4") +renv::install("assertthat@0.2.1") +renv::install("cli@1.1.0") +renv::install("fansi@0.4.0") +renv::install("utf8@1.1.4") +renv::install("digest@0.6.18") +renv::install("glue@1.3.1") 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"illuminaHumanv3.db", + "illuminaHumanv4.db", + "illuminaMousev1.db", + "illuminaMousev1p1.db", + "illuminaMousev2.db", + "illuminaRatv1.db" +) diff --git a/workers/R/dependencies/no_op/renv.lock b/workers/R/dependencies/no_op/renv.lock new file mode 100644 index 000000000..7c1ebdefc --- /dev/null +++ b/workers/R/dependencies/no_op/renv.lock @@ -0,0 +1,1613 @@ +{ + "R": { + "Version": "3.4.4", + "Repositories": [ + { + "Name": "Bioconductor", + "URL": "https://bioconductor.org/packages/3.6/bioc" + }, + { + "Name": "BioconductorAnnotation", + "URL": "https://bioconductor.org/packages/3.6/data/annotation" + }, + { + "Name": "BioconductorExperiment", + "URL": "https://bioconductor.org/packages/3.6/data/experiment" + }, + { + "Name": "CRAN", + "URL": "https://cloud.r-project.org" + } + ] + }, + "Packages": { + "AnnotationDbi": { + "Package": "AnnotationDbi", + "Version": "1.40.0", + "Source": "Bioconductor", + "Hash": "abb20d74ae5ea08a8d9616a6f1bbaca5", + "Requirements": [ + "Biobase", + 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workers/R/dependencies/no_op/install_gene_convert.R . -RUN Rscript install_gene_convert.R +COPY workers/R/dependencies/no_op/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir numpy && \ - pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Noop-specific. RUN mkdir -p gene_indexes - WORKDIR /home/user/gene_indexes - ENV ID_REFINERY_URL=https://zenodo.org/record/1410647/files/all_1536267482.zip RUN curl -O $ID_REFINERY_URL && \ echo $ID_REFINERY_URL > /etc/identifier_refinery_url && \ @@ -39,6 +33,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION From 98e7a6df5c13449c76bc1fe8f30e19eef83c84b4 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 9 Mar 2023 11:05:33 -0800 Subject: [PATCH 084/220] Update illumina docker image and R dependencies. --- workers/R/dependencies/illumina/renv.R | 49 + workers/R/dependencies/illumina/renv.lock | 1587 +++++++++++++++++++++ workers/dockerfiles/Dockerfile.illumina | 14 +- 3 files changed, 1642 insertions(+), 8 deletions(-) create mode 100644 workers/R/dependencies/illumina/renv.R create mode 100644 workers/R/dependencies/illumina/renv.lock diff --git a/workers/R/dependencies/illumina/renv.R b/workers/R/dependencies/illumina/renv.R new file mode 100644 index 000000000..acebff1eb --- /dev/null +++ b/workers/R/dependencies/illumina/renv.R @@ -0,0 +1,49 @@ +renv::install("iterators@1.0.10") +renv::install("codetools@0.2-16") +renv::install("foreach@1.4.4") +renv::install("doParallel@1.0.11") +renv::install("data.table@1.11.0") +renv::install("getopt@1.20.3") +renv::install("optparse@1.4.4") +renv::install("lazyeval@0.2.1") +renv::install("rlang@0.3.1") +renv::install("glue@1.3.1") +renv::install("digest@0.6.18") +renv::install("crayon@1.3.4") +renv::install("assertthat@0.2.1") +renv::install("fansi@0.4.0") +renv::install("utf8@1.1.4") +renv::install("pillar@1.3.1") +renv::install("pkgconfig@2.0.2") +renv::install("pillar@1.3.1") +renv::install("tibble@2.0.1") +renv::install("R6@2.4.0") +renv::install("Rcpp@1.0.1") +renv::install("magrittr@1.5") +renv::install("tidyselect@0.2.5") +renv::install("lifecycle@1.0.3") +renv::install("tibble@2.0.1") +renv::install("dplyr@1.0.0") 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workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ @@ -27,6 +23,8 @@ COPY workers/ . COPY workers/data_refinery_workers/processors/detect_database.R . COPY workers/illumina_probe_maps/ probe_maps/ +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION From 75e57c3a3d6842e8c40277ce81ef00bf045086c2 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 9 Mar 2023 15:04:25 -0800 Subject: [PATCH 085/220] Update compendia Docker image and R dependencies. --- workers/R/dependencies/compendia/renv.R | 15 + workers/R/dependencies/compendia/renv.lock | 1440 ++++++++++++++++++++ workers/dockerfiles/Dockerfile.compendia | 32 +- 3 files changed, 1465 insertions(+), 22 deletions(-) create mode 100644 workers/R/dependencies/compendia/renv.R create mode 100644 workers/R/dependencies/compendia/renv.lock diff --git a/workers/R/dependencies/compendia/renv.R b/workers/R/dependencies/compendia/renv.R new file mode 100644 index 000000000..a6523cf18 --- /dev/null +++ b/workers/R/dependencies/compendia/renv.R @@ -0,0 +1,15 @@ +renv::install("codetools@0.2-15") +renv::install("iterators@1.0.10") 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+ "Hash": "308013098befe37484df72c39cf90d6e", + "Requirements": [ + "cli", + "withr" + ] + }, + "spatial": { + "Package": "spatial", + "Version": "7.3-11", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "f27a078d834614513608da2c08031130", + "Requirements": [] + }, + "stringi": { + "Package": "stringi", + "Version": "1.7.12", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "ca8bd84263c77310739d2cf64d84d7c9", + "Requirements": [] + }, + "stringr": { + "Package": "stringr", + "Version": "1.5.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "671a4d384ae9d32fc47a14e98bfa3dc8", + "Requirements": [ + "cli", + "glue", + "lifecycle", + "magrittr", + "rlang", + "stringi", + "vctrs" + ] + }, + "survival": { + "Package": "survival", + "Version": "2.41-3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "60b1da51f5e3556fd177170a1aa40b49", + "Requirements": [ + "Matrix" + ] + }, + "sys": { + "Package": "sys", + "Version": "3.4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "b227d13e29222b4574486cfcbde077fa", + "Requirements": [] + }, + "testthat": { + "Package": "testthat", + "Version": "2.3.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "0829b987b8961fb07f3b1b64a2fbc495", + "Requirements": [ + "R6", + "cli", + "crayon", + "digest", + "ellipsis", + "evaluate", + "magrittr", + "pkgload", + "praise", + "rlang", + "withr" + ] + }, + "tibble": { + "Package": "tibble", + "Version": "3.1.8", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "56b6934ef0f8c68225949a8672fe1a8f", + "Requirements": [ + "fansi", + "lifecycle", + "magrittr", + "pillar", + "pkgconfig", + "rlang", + "vctrs" + ] + }, + "tidyselect": { + "Package": "tidyselect", + "Version": "1.2.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "79540e5fcd9e0435af547d885f184fd5", + "Requirements": [ + "cli", + "glue", + "lifecycle", + "rlang", + "vctrs", + "withr" + ] + }, + "tximport": { + "Package": "tximport", + "Version": "1.6.0", + "Source": "Bioconductor", + "Hash": "2c824ac20bbb34bba92a9b5b0d600fec", + "Requirements": [] + }, + "usethis": { + "Package": "usethis", + "Version": "1.6.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "e1985f5a9985fea2e338fa7eb99018ca", + "Requirements": [ + "cli", + "clipr", + "crayon", + "curl", + "desc", + "fs", + "gh", + "git2r", + "glue", + "purrr", + "rematch2", + "rlang", + "rprojroot", + "rstudioapi", + "whisker", + "withr", + "yaml" + ] + }, + "utf8": { + "Package": "utf8", + "Version": "1.2.3", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "1fe17157424bb09c48a8b3b550c753bc", + "Requirements": [] + }, + "vctrs": { + "Package": "vctrs", + "Version": "0.5.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "e4ffa94ceed5f124d429a5a5f0f5b378", + "Requirements": [ + "cli", + "glue", + "lifecycle", + "rlang" + ] + }, + "whisker": { + "Package": "whisker", + "Version": "0.4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "ca970b96d894e90397ed20637a0c1bbe", + "Requirements": [] + }, + "withr": { + "Package": "withr", + "Version": "2.5.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "c0e49a9760983e81e55cdd9be92e7182", + "Requirements": [] + }, + "xfun": { + "Package": "xfun", + "Version": "0.36", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "f5baec54606751aa53ac9c0e05848ed6", + "Requirements": [] + }, + "xml2": { + "Package": "xml2", + "Version": "1.3.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "d4d71a75dd3ea9eb5fa28cc21f9585e2", + "Requirements": [] + }, + "xopen": { + "Package": "xopen", + "Version": "1.0.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "6c85f015dee9cc7710ddd20f86881f58", + "Requirements": [ + "processx" + ] + }, + "yaml": { + "Package": "yaml", + "Version": "2.2.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "2826c5d9efb0a88f657c7a679c7106db", + "Requirements": [] + }, + "zlibbioc": { + "Package": "zlibbioc", + "Version": "1.24.0", + "Source": "Bioconductor", + "Hash": "75640bcdaa390770a672332c904642db", + "Requirements": [] + } + } +} diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 8790d859c..6ab413714 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -13,8 +13,6 @@ ENV DEBIAN_FRONTEND=noninteractive # For whatever reason this worked and 'en_US.UTF-8' did not. ENV LANG=C.UTF-8 -COPY workers/CRAN.gpg . - # RUN apt-key adv --fetch-keys https://developer.download.nvidia.com/compute/cuda/repos/ubuntu1804/x86_64/3bf863cc.pub # via https://github.com/ilikenwf/apt-fast/issues/85#issuecomment-261640099 RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ @@ -29,13 +27,9 @@ RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ add-apt-repository ppa:savoury1/llvm-defaults-10 && \ apt-get update -qq && \ apt-get install --no-install-recommends -y apt-fast apt-transport-https gpg-agent tzdata && \ - apt-key add CRAN.gpg && \ - echo "deb https://cloud.r-project.org/bin/linux/ubuntu bionic-cran35/" \ - >> /etc/apt/sources.list.d/added_repos.list && \ apt-fast update -qq && \ apt-fast install -y \ build-essential \ - ccache \ cmake \ curl \ cython3 \ @@ -55,40 +49,32 @@ RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ mercurial \ pkg-config \ python3-pip \ + python3-pybind11 \ python3.8 \ python3.8-dev \ r-base-core \ wget && \ apt-get clean && \ - rm CRAN.gpg && \ rm -rf /var/lib/apt/lists/* && \ ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ update-alternatives --install /usr/bin/python3 python3 \ /usr/bin/python3.8 1 && \ + pip3 install --upgrade pip setuptools && \ groupadd user && \ useradd --create-home --home-dir /home/user -g user user WORKDIR /home/user -# We need a few special packages for QN. ENV R_LIBS=/usr/local/lib/R/site-library -COPY common/R/install_devtools.R . -RUN Rscript install_devtools.R - -COPY workers/R/dependencies/qn/cran/dependencies.R . -RUN Rscript dependencies.R - -COPY workers/R/dependencies/qn/bioc/dependencies.R . -RUN Rscript dependencies.R -# End QN-specific. +COPY workers/R/dependencies/compendia/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir --upgrade pip && \ - pip3 install --ignore-installed --no-cache-dir nose numpy scipy matplotlib \ - pandas==0.25.3 scikit-learn sympy rpy2===3.4.5 tzlocal fancySVD && \ - pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ - pip3 install --ignore-installed --no-cache-dir numpy==1.16.0 +RUN pip3 install --ignore-installed --no-cache-dir nose numpy scipy matplotlib \ + pandas==0.25.3 scikit-learn sympy tzlocal fancySVD && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Install phantomjs. RUN wget -q "https://bitbucket.org/ariya/phantomjs/downloads/\ @@ -106,6 +92,8 @@ COPY .boto .boto COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION From 531149906d74ee1b448997868c658da004d7115f Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 9 Mar 2023 15:18:28 -0800 Subject: [PATCH 086/220] Update affymetrix dependencies. --- .../affymetrix/install_ensg_pkgs.R | 15 +- workers/R/dependencies/affymetrix/renv.R | 236 + workers/R/dependencies/affymetrix/renv.lock | 4545 +++++++++++++++++ workers/ccache.conf | 5 - workers/dockerfiles/Dockerfile.affymetrix | 46 +- .../dockerfiles/Dockerfile.affymetrix_local | 4 + 6 files changed, 4807 insertions(+), 44 deletions(-) create mode 100644 workers/R/dependencies/affymetrix/renv.R create mode 100644 workers/R/dependencies/affymetrix/renv.lock delete mode 100644 workers/ccache.conf diff --git a/workers/R/dependencies/affymetrix/install_ensg_pkgs.R b/workers/R/dependencies/affymetrix/install_ensg_pkgs.R index 5de25a262..da75b17a8 100644 --- a/workers/R/dependencies/affymetrix/install_ensg_pkgs.R +++ b/workers/R/dependencies/affymetrix/install_ensg_pkgs.R @@ -1,16 +1,17 @@ options(Ncpus = parallel::detectCores()) -install.packages("xml2") + library("xml2") + ensg_url <- "http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/22.0.0/ensg.asp" html_content <- read_html(ensg_url) # The second table in the html is the main table that lists all ensg packages. data_table <- xml_find_all(html_content, ".//table")[[2]] -# Extract data rows from the second table: +# Extract data rows from the second table. data_rows <- xml_children(data_table)[3:xml_length(data_table)] -# These two global variables will be modified by save_chip_pkg(): +# These two global variables will be modified by save_chip_pkg(). chips <- c() pkg_urls <- c() @@ -34,15 +35,15 @@ save_chip_pkg <- function(row) { } } -# Extract data of interest out of each row +# Extract data of interest out of each row. lapply(data_rows, save_chip_pkg) -# Write chips and pkg_urls to a tab-delimited file +# Write chips and pkg_urls to a tab-delimited file. output_filename <- "/home/user/r_ensg_probe_pkgs.txt" write.table(list(chips, pkg_urls), file = output_filename, quote = FALSE, row.names = FALSE, col.names = FALSE, sep = "\t" ) -# Install these ensg packages -lapply(pkg_urls, devtools::install_url) +# Install these ensg packages. +lapply(pkg_urls, install.packages) diff --git a/workers/R/dependencies/affymetrix/renv.R b/workers/R/dependencies/affymetrix/renv.R new file mode 100644 index 000000000..ecc78f904 --- /dev/null +++ b/workers/R/dependencies/affymetrix/renv.R @@ -0,0 +1,236 @@ +renv::install("bit@1.1-14") +renv::install("XML@3.98-1.10") +renv::install("bitops@1.0-6") +renv::install("RCurl@1.95-4.10") +renv::install("pkgconfig@2.0.1") +renv::install("backports@1.1.4") +renv::install("rlang@0.4.0") +renv::install("zeallot@0.1.0") +renv::install("glue@1.3.1") +renv::install("digest@0.6.19") +renv::install("vctrs@0.1.0") +renv::install("magrittr@1.5") +renv::install("assertthat@0.2.1") +renv::install("prettyunits@1.0.2") +renv::install("blob@1.1.1") +renv::install("memoise@2.0.1") +renv::install("DBI@1.0.0") +renv::install("Rcpp@1.0.7") +renv::install("bit64@0.9-7") +renv::install("RSQLite@2.0") +renv::install("fansi@0.4.0") +renv::install("crayon@1.3.4") +renv::install("cli@1.1.0") +renv::install("utf8@1.1.4") +renv::install("pillar@1.4.2") +renv::install("lifecycle@1.0.3") +renv::install("tibble@1.4.2") +renv::install("xtable@1.8-2") +renv::install("tidyselect@0.2.5") +renv::install("R6@2.4.0") +renv::install("dplyr@1.0.2") +renv::install("stringi@1.5.3") +renv::install("sys@3.2") +renv::install("askpass@1.1") +renv::install("openssl@2.0.3") +renv::install("curl@3.3") +renv::install("mime@0.7") +renv::install("httr@1.4.2") +renv::install("hms@0.4.2") +renv::install("clipr@0.6.0") +renv::install("readr@1.3.1") +renv::install("xml2@1.3.1") +renv::install("locfit@1.5-9.2") +renv::install("getopt@1.20.3") +renv::install("optparse@1.4.4") + +renv::install( + "affy", + "affyio", + "AnnotationDbi", + "ff@2.2-14", + "genefilter", + "GEOquery", + "limma", + "oligo", + "oligoClasses", + "preprocessCore", + "purrr@0.3.5", + "SCAN.UPC", + "sva", + "tidyr@1.1.0", + "zlibbioc" +) + +renv::install( + "pd.081229.hg18.promoter.medip.hx1", + "pd.2006.07.18.hg18.refseq.promoter", + "pd.2006.07.18.mm8.refseq.promoter", + "pd.2006.10.31.rn34.refseq.promoter", + "pd.ag", + "pd.aragene.1.0.st", + "pd.aragene.1.1.st", + "pd.atdschip.tiling", + "pd.ath1.121501", + "pd.barley1", + "pd.bovgene.1.0.st", + "pd.bovgene.1.1.st", + "pd.bovine", + "pd.bsubtilis", + "pd.cangene.1.0.st", + "pd.cangene.1.1.st", + "pd.canine.2", + "pd.canine", + "pd.celegans", + "pd.charm.hg18.example", + "pd.chicken", + "pd.chigene.1.0.st", + "pd.chigene.1.1.st", + "pd.chogene.2.0.st", + "pd.chogene.2.1.st", + "pd.citrus", + "pd.clariom.d.human", + "pd.clariom.s.human.ht", + "pd.clariom.s.human", + "pd.clariom.s.mouse.ht", + "pd.clariom.s.mouse", + "pd.clariom.s.rat.ht", + "pd.clariom.s.rat", + "pd.cotton", + "pd.cyngene.1.0.st", + "pd.cyngene.1.1.st", + "pd.cyrgene.1.0.st", + "pd.cyrgene.1.1.st", + "pd.cytogenetics.array", + "pd.drogene.1.0.st", + "pd.drogene.1.1.st", + "pd.drosgenome1", + "pd.drosophila.2", + "pd.e.coli.2", + "pd.ecoli.asv2", + "pd.ecoli", + "pd.elegene.1.0.st", + "pd.elegene.1.1.st", + "pd.equgene.1.0.st", + "pd.equgene.1.1.st", + "pd.feinberg.hg18.me.hx1", + "pd.feinberg.mm8.me.hx1", + "pd.felgene.1.0.st", + "pd.felgene.1.1.st", + "pd.fingene.1.0.st", + "pd.fingene.1.1.st", + "pd.genomewidesnp.5", + "pd.genomewidesnp.6", + "pd.guigene.1.0.st", + "pd.guigene.1.1.st", + "pd.hc.g110", + "pd.hg.focus", + "pd.hg.u133.plus.2", + "pd.hg.u133a.2", + "pd.hg.u133a.tag", + "pd.hg.u133a", + "pd.hg.u133b", + "pd.hg.u219", + "pd.hg.u95a", + "pd.hg.u95av2", + "pd.hg.u95b", + "pd.hg.u95c", + "pd.hg.u95d", + "pd.hg.u95e", + "pd.hg18.60mer.expr", + "pd.ht.hg.u133.plus.pm", + "pd.ht.hg.u133a", + "pd.ht.mg.430a", + "pd.hta.2.0", + "pd.hu6800", + "pd.huex.1.0.st.v2", + "pd.hugene.1.0.st.v1", + "pd.hugene.1.1.st.v1", + "pd.hugene.2.0.st", + "pd.hugene.2.1.st", + "pd.maize", + "pd.mapping250k.nsp", + "pd.mapping250k.sty", + "pd.mapping50k.hind240", + "pd.mapping50k.xba240", + "pd.margene.1.0.st", + "pd.margene.1.1.st", + "pd.medgene.1.0.st", + "pd.medgene.1.1.st", + "pd.medicago", + "pd.mg.u74a", + "pd.mg.u74av2", + "pd.mg.u74b", + "pd.mg.u74bv2", + "pd.mg.u74c", + "pd.mg.u74cv2", + "pd.mirna.1.0", + "pd.mirna.2.0", + "pd.mirna.3.0", + "pd.mirna.3.1", + "pd.mirna.4.0", + "pd.moe430a", + "pd.moe430b", + "pd.moex.1.0.st.v1", + "pd.mogene.1.0.st.v1", + "pd.mogene.1.1.st.v1", + "pd.mogene.2.0.st", + "pd.mogene.2.1.st", + "pd.mouse430.2", + "pd.mouse430a.2", + "pd.mta.1.0", + "pd.mu11ksuba", + "pd.mu11ksubb", + "pd.nugo.hs1a520180", + "pd.nugo.mm1a520177", + "pd.ovigene.1.0.st", + "pd.ovigene.1.1.st", + "pd.pae.g1a", + "pd.plasmodium.anopheles", + "pd.poplar", + "pd.porcine", + "pd.porgene.1.0.st", + "pd.porgene.1.1.st", + "pd.rabgene.1.0.st", + "pd.rabgene.1.1.st", + "pd.rae230a", + "pd.rae230b", + "pd.raex.1.0.st.v1", + "pd.ragene.1.0.st.v1", + "pd.ragene.1.1.st.v1", + "pd.ragene.2.0.st", + "pd.ragene.2.1.st", + "pd.rat230.2", + "pd.rcngene.1.0.st", + "pd.rcngene.1.1.st", + "pd.rg.u34a", + "pd.rg.u34b", + "pd.rg.u34c", + "pd.rhegene.1.0.st", + "pd.rhegene.1.1.st", + "pd.rhesus", + "pd.rice", + "pd.rjpgene.1.0.st", + "pd.rjpgene.1.1.st", + "pd.rn.u34", + "pd.rta.1.0", + "pd.rusgene.1.0.st", + "pd.rusgene.1.1.st", + "pd.s.aureus", + "pd.soybean", + "pd.soygene.1.0.st", + "pd.soygene.1.1.st", + "pd.sugar.cane", + "pd.tomato", + "pd.u133.x3p", + "pd.vitis.vinifera", + "pd.wheat", + "pd.x.laevis.2", + "pd.x.tropicalis", + "pd.xenopus.laevis", + "pd.yeast.2", + "pd.yg.s98", + "pd.zebgene.1.0.st", + "pd.zebgene.1.1.st", + "pd.zebrafish" +) diff --git 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"035fba89d0c86e2113120f93301b98ad", + "Requirements": [ + "cli", + "diffobj", + "fansi", + "glue", + "rematch2", + "rlang", + "tibble" + ] + }, + "whisker": { + "Package": "whisker", + "Version": "0.4", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "ca970b96d894e90397ed20637a0c1bbe", + "Requirements": [] + }, + "withr": { + "Package": "withr", + "Version": "2.5.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "c0e49a9760983e81e55cdd9be92e7182", + "Requirements": [] + }, + "xfun": { + "Package": "xfun", + "Version": "0.36", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "f5baec54606751aa53ac9c0e05848ed6", + "Requirements": [] + }, + "xml2": { + "Package": "xml2", + "Version": "1.3.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "921dce7c0ec595ed85e77683d3e6c8b6", + "Requirements": [] + }, + "xopen": { + "Package": "xopen", + "Version": "1.0.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "6c85f015dee9cc7710ddd20f86881f58", + "Requirements": [ + "processx" + ] + }, + "xtable": { + "Package": "xtable", + "Version": "1.8-2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "4739f99be2ab40fe89cbd00365c98294", + "Requirements": [] + }, + "yaml": { + "Package": "yaml", + "Version": "2.2.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "2826c5d9efb0a88f657c7a679c7106db", + "Requirements": [] + }, + "zeallot": { + "Package": "zeallot", + "Version": "0.1.0", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "ee9b643aa8331c45d8d82eb3a137c9bc", + "Requirements": [] + }, + "zip": { + "Package": "zip", + "Version": "2.2.2", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "c42bfcec3fa6a0cce17ce1f8bc684f88", + "Requirements": [] + }, + "zlibbioc": { + "Package": "zlibbioc", + "Version": "1.24.0", + "Source": "Bioconductor", + "Hash": "75640bcdaa390770a672332c904642db", + "Requirements": [] + } + } +} diff --git a/workers/ccache.conf b/workers/ccache.conf deleted file mode 100644 index cde43e665..000000000 --- a/workers/ccache.conf +++ /dev/null @@ -1,5 +0,0 @@ -max_size = 5.0G -# important for R CMD INSTALL *.tar.gz as tarballs are expanded freshly -> fresh ctime -sloppiness = include_file_ctime -# also important as the (temp.) directory name will differ -hash_dir = false diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index e90300240..6ac42f4ef 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -1,52 +1,34 @@ -FROM ccdlstaging/dr_worker_base:latest +FROM ccdlstaging/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -# Install and set up ccache. -RUN apt-get update && \ - apt-get install --no-install-recommends -y ccache && \ - for i in c++ cc g++ gcc; do ln -fs /usr/bin/ccache /usr/local/bin/$i; done -COPY workers/ccache.conf /root/.ccache/ccache.conf -ENV PATH="/usr/local/bin:${PATH}" - -COPY workers/R/dependencies/affymetrix/cran/dependencies.R dependencies.R -RUN Rscript dependencies.R +COPY workers/R/dependencies/affymetrix/renv.lock . +COPY workers/R/renv_load.R . +RUN Rscript renv_load.R COPY workers/R/dependencies/affymetrix/install_ensg_pkgs.R . +RUN Rscript install_ensg_pkgs.R -COPY workers/R/dependencies/affymetrix/bioc/dependencies.R dependencies.R -RUN Rscript dependencies.R - -RUN pip3 install --ignore-installed --upgrade pip setuptools && \ - # Install this one here instead of via requirements.txt because not - # all processors need it. - pip3 install --ignore-installed rpy2==3.4.5 +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) +RUN pip3 install --ignore-installed --no-cache-dir \ + common/$(ls common -1 | sort --version-sort | tail -1) -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed -r requirements.txt && \ - # Clear out the pip3 cache. - rm -r /root/.cache +COPY .boto .boto +COPY config/ config/ +COPY workers/ . + +RUN rm -rf /root/.cache/* ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION -# Print compiler cache stats (the cache hit ratio should be ~90%). -RUN ccache --show-stats && \ - # Clear out the ccache. - ccache --clear - USER user -COPY .boto .boto -COPY config/ config/ -COPY workers/ . - ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.affymetrix_local b/workers/dockerfiles/Dockerfile.affymetrix_local index 08db9e197..7fe881ab0 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix_local +++ b/workers/dockerfiles/Dockerfile.affymetrix_local @@ -3,6 +3,8 @@ FROM ccdlstaging/dr_affymetrix:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] +USER root + WORKDIR /home/user # Remove the version of common already installed. @@ -17,6 +19,8 @@ RUN pip3 install --ignore-installed --no-cache-dir \ COPY config/ config/ COPY workers/ . +RUN rm -rf /root/.cache/* + ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION From 035959b31d78ff81cd317585944ab62de804c8d9 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Thu, 16 Mar 2023 16:45:49 -0400 Subject: [PATCH 087/220] pull salmontools test data from s3 --- workers/run_tests.sh | 19 ++++++++++++------- 1 file changed, 12 insertions(+), 7 deletions(-) diff --git a/workers/run_tests.sh b/workers/run_tests.sh index a750b502a..185a98394 100755 --- a/workers/run_tests.sh +++ b/workers/run_tests.sh @@ -77,13 +77,18 @@ if [ -z "$tag" ] || [ "$tag" = "salmon" ]; then rm "$volume_directory"/salmon_tests.tar.gz fi - # Download salmontools test data - rm -rf "$volume_directory"/salmontools/ - git clone https://github.com/dongbohu/salmontools_tests.git "$volume_directory"/salmontools - - # Download tximport test data - rm -rf "$volume_directory"/tximport_test/ - git clone https://github.com/dongbohu/tximport_test.git "$volume_directory"/tximport_test + # salmontools test data + salmontools_test_zip="$test_data_repo/salmontools_test_data.tar.gz" + salmontools_test_dir="$volume_directory/salmontools" + + # Clean the test data directory + rm -rf "$salmontools_test_dir" + mkdir -p "$salmontools_test_dir" + + echo "Downloading 'salmontools' test data..." + wget -q -O "$volume_directory"/salmontools.tar.gz "$salmontools_test_zip" + tar xzf "$volume_directory"/salmontools.tar.gz -C "$volume_directory" + rm "$volume_directory"/salmontools.tar.gz # Make sure data for Salmon test is downloaded from S3. rna_seq_test_raw_dir="$volume_directory/raw/TEST/SALMON" From 9969016937ea28b4923620eb73825fd05f265c3d Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 29 Mar 2023 15:33:27 -0700 Subject: [PATCH 088/220] Remove old comment. --- common/dockerfiles/Dockerfile.migrations | 1 - 1 file changed, 1 deletion(-) diff --git a/common/dockerfiles/Dockerfile.migrations b/common/dockerfiles/Dockerfile.migrations index f91316128..a7e54b019 100644 --- a/common/dockerfiles/Dockerfile.migrations +++ b/common/dockerfiles/Dockerfile.migrations @@ -12,7 +12,6 @@ COPY common/requirements.txt . RUN pip install --ignore-installed --no-cache-dir -r requirements.txt COPY common/ . -# Needed for migration 0006. COPY config config ARG SYSTEM_VERSION From bb88c46fc5187b347fb8a8a8e82430930b05c264 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 30 Mar 2023 08:58:11 -0700 Subject: [PATCH 089/220] Add a TODO. --- foreman/data_refinery_foreman/surveyor/sdrf.csv | 13 +++++++++++++ foreman/requirements.in | 1 + 2 files changed, 14 insertions(+) create mode 100644 foreman/data_refinery_foreman/surveyor/sdrf.csv diff --git a/foreman/data_refinery_foreman/surveyor/sdrf.csv b/foreman/data_refinery_foreman/surveyor/sdrf.csv new file mode 100644 index 000000000..d01d4cb2d --- /dev/null +++ b/foreman/data_refinery_foreman/surveyor/sdrf.csv @@ -0,0 +1,13 @@ +Source Name Description Material Type Characteristics[organism] Characteristics[organism part] Characteristics[sex] Characteristics[developmental stage] Characteristics[age] Unit [time unit] Protocol REF Protocol REF Protocol REF Protocol REF Extract Name Protocol REF Labeled Extract Name Label Protocol REF Assay Name Technology Type Array Design REF Protocol REF Image File Protocol REF Array Data File Comment [ArrayExpress FTP file] Protocol REF Derived Array Data File Comment [Derived ArrayExpress FTP file] Factor Value[cell type] Factor Value[individual] Factor Value[test result] +donor A islets �Islets from 54 years old female at 90% purity. Time from islet preparation to culture initiation: 96 hours. Provided by the University of Miami. cell Homo sapiens islet female adult 54 year P-MTAB-41854 P-MTAB-41858 donor A islets RNA P-MTAB-41859 donor A islets LEX biotin P-MTAB-41859 1007409-C30057 array assay A-AFFY-1 P-MTAB-41860 C30057.DAT P-MTAB-41861 C30057.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.raw.1.zip P-MTAB-41862 C30057.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.processed.1.zip freshly isolated A NA +donor A islets �Islets from 54 years old female at 90% purity. Time from islet preparation to culture initiation: 96 hours. Provided by the University of Miami. cell Homo sapiens islet female adult 54 year P-MTAB-41854 P-MTAB-41855 P-MTAB-41858 donor A expanded cells RNA P-MTAB-41859 donor A expanded cells LEX biotin P-MTAB-41859 1007409-C30058 array assay A-AFFY-1 P-MTAB-41860 C30058.DAT P-MTAB-41861 C30058.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.raw.1.zip P-MTAB-41862 C30058.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.processed.1.zip expanded A NA +donor A islets �Islets from 54 years old female at 90% purity. Time from islet preparation to culture initiation: 96 hours. Provided by the University of Miami. cell Homo sapiens islet female adult 54 year P-MTAB-41854 P-MTAB-41855 P-MTAB-41856 P-MTAB-41858 donor A differentiated cells RNA P-MTAB-41859 donor A differentiated cells LEX biotin P-MTAB-41859 1009003-C30059 array assay A-AFFY-1 P-MTAB-41860 C30059.DAT P-MTAB-41861 C30059.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.raw.1.zip P-MTAB-41862 C30059.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.processed.1.zip differentiated A successful +donor B islets Islets from 38 years old male. Time from islet preparation to culture initiation: 96 hours. Provided by the North West Tissue Center Seattle. cell Homo sapiens islet male adult 38 year P-MTAB-41854 P-MTAB-41855 P-MTAB-41858 donor B expanded cells RNA P-MTAB-41859 donor B expanded cells LEX biotin P-MTAB-41859 1009003-C30060 array assay A-AFFY-1 P-MTAB-41860 C30060.DAT P-MTAB-41861 C30060.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.raw.1.zip P-MTAB-41862 C30060.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.processed.1.zip expanded B NA +donor B islets Islets from 38 years old male. Time from islet preparation to culture initiation: 96 hours. Provided by the North West Tissue Center Seattle. cell Homo sapiens islet male adult 38 year P-MTAB-41854 P-MTAB-41855 P-MTAB-41856 P-MTAB-41858 donor B differentiated cells RNA P-MTAB-41859 donor B differentiated cells LEX biotin P-MTAB-41859 1009003-C30061 array assay A-AFFY-1 P-MTAB-41860 C30061.DAT P-MTAB-41861 C30061.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.raw.1.zip P-MTAB-41862 C30061.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MTAB/E-MTAB-3050/E-MTAB-3050.processed.1.zip differentiated B unsuccessful + + + + +Source Name Characteristics [Age] Characteristics [GeneticModification] Characteristics [Organism] Term Source REF Term Accession Number Characteristics [BioSourceType] Characteristics [Genotype] Term Source REF Term Accession Number Characteristics [DevelopmentalStage] Term Source REF Term Accession Number Protocol REF Extract Name Material Type Protocol REF Labeled Extract Name Label Material Type Protocol REF Hybridization Name Array Design REF Protocol REF Factor Value [GENOTYPE] Term Source REF Term Accession Number Scan Name Array Data File Comment [ArrayExpress FTP file] +SAMPLE229091SUB6092 48 hours induced_mutation Danio rerio EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_7955 fresh_sample RPL11 mutation embryo EFO EFO_0001367 P-MTAB-5485 SAMPLE229091SUB6092 total_RNA P-AFFY-2 SAMPLE229091SUB6092 biotin unknown Affymetrix:Protocol:Hybridization-Unknown SAMPLE229091SUB6092 A-AFFY-38 P-NGEN-13 RPL11 mutation 135354-135352_ExpPt1.CEL 135354-135352_ExpPt1.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-2381/E-MEXP-2381.raw.1.zip +SAMPLE229092SUB6092 48 hours Danio rerio EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_7955 control EFO EFO_0001461 P-MTAB-5485 SAMPLE229092SUB6092 total_RNA P-AFFY-2 SAMPLE229092SUB6092 biotin unknown Affymetrix:Protocol:Hybridization-Unknown SAMPLE229092SUB6092 A-AFFY-38 P-NGEN-13 control EFO EFO_0001461 135353-135351_CtrlPt1.CEL 135353-135351_CtrlPt1.CEL ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-2381/E-MEXP-2381.raw.1.zip diff --git a/foreman/requirements.in b/foreman/requirements.in index 20b88a17f..3d92224a1 100644 --- a/foreman/requirements.in +++ b/foreman/requirements.in @@ -8,6 +8,7 @@ python-dateutil raven pyrefinebio>=0.4.7 tblib +# TODO(ark): make sure DRF is required. djangorestframework<3.14.0 # Can't go above 6.X without upgrading elasticsearch django-elasticsearch-dsl==6.5.0 From 231f473396677ed5b30470e2bdfc581e042116af Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 31 Mar 2023 13:55:56 -0700 Subject: [PATCH 090/220] Extract compendia requirements. --- workers/data_refinery_workers/requirements/compendia.txt | 9 +++++++++ workers/dockerfiles/Dockerfile.compendia | 9 +++++---- 2 files changed, 14 insertions(+), 4 deletions(-) create mode 100644 workers/data_refinery_workers/requirements/compendia.txt diff --git a/workers/data_refinery_workers/requirements/compendia.txt b/workers/data_refinery_workers/requirements/compendia.txt new file mode 100644 index 000000000..10e91e3c6 --- /dev/null +++ b/workers/data_refinery_workers/requirements/compendia.txt @@ -0,0 +1,9 @@ +nose +numpy +scipy +matplotlib +pandas==0.25.3 +scikit-learn +sympy +tzlocal +fancySVD diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 6ab413714..7b8240b78 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -71,10 +71,11 @@ COPY workers/R/dependencies/compendia/renv.lock . COPY workers/R/renv_load.R . RUN Rscript renv_load.R -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir nose numpy scipy matplotlib \ - pandas==0.25.3 scikit-learn sympy tzlocal fancySVD && \ - pip3 install --ignore-installed --no-cache-dir -r requirements.txt +COPY workers/data_refinery_workers/requirements/compendia.txt requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt + +COPY workers/data_refinery_workers/processors/requirements.txt requirements.txt +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Install phantomjs. RUN wget -q "https://bitbucket.org/ariya/phantomjs/downloads/\ From 39af92af12c6f5ae5e8c6fca07350f50900ed0ef Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 3 Apr 2023 11:54:53 -0700 Subject: [PATCH 091/220] Add keys README. --- workers/keys/README.md | 3 +++ 1 file changed, 3 insertions(+) create mode 100644 workers/keys/README.md diff --git a/workers/keys/README.md b/workers/keys/README.md new file mode 100644 index 000000000..bf95182d9 --- /dev/null +++ b/workers/keys/README.md @@ -0,0 +1,3 @@ +`asperaweb_id_dsa.openssh` is a publicly available "private" key required by ascp. +See https://www.ibm.com/docs/en/aspera-on-cloud?topic=ak-fasp-security-aspera-connect-transfer-client-authorized-keys-aspshell +for more details. From 307143db59b558d9358a185372bc1513c345caf9 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 3 Apr 2023 14:05:30 -0700 Subject: [PATCH 092/220] Address review comments. --- .../gatherer/agents/ae_agent.py | 3 --- .../gatherer/agents/geo_agent.py | 3 --- .../gatherer/agents/rnaseq_agent.py | 3 --- .../management/commands/gather_accessions.py | 14 +++++++------- 4 files changed, 7 insertions(+), 16 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py b/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py index df8399bca..4328f0713 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py +++ b/foreman/data_refinery_foreman/gatherer/agents/ae_agent.py @@ -29,9 +29,6 @@ class AEAgent(AccessionAgentBase): SOURCE_NAME = "microarray-ae" TECHNOLOGY = "microarray" - def __str__(self): - return "MicroArray ArrayExpress accession agent" - def __str__(self): return "MicroArray ArrayExpress accession agent" diff --git a/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py b/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py index 2eb7f7b6f..22a3bd0bd 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py +++ b/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py @@ -30,9 +30,6 @@ class GEOAgent(AccessionAgentBase): SOURCE_NAME = "microarray-geo" TECHNOLOGY = "microarray" - def __str__(self): - return "MicroArray GEO accession agent" - def __str__(self): return "MicroArray GEO accession agent" diff --git a/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py index 9f2f54e3a..3fcb87fbb 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py +++ b/foreman/data_refinery_foreman/gatherer/agents/rnaseq_agent.py @@ -31,9 +31,6 @@ class RNASeqAgent(AccessionAgentBase): SOURCE_NAME = "rna-seq" TECHNOLOGY = "rna-seq" - def __str__(self): - return "RNA-Seq accession agent" - def __str__(self): return "RNA-Seq accession agent" diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 3e50dac28..3c1ac8f73 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -27,8 +27,6 @@ class Command(BaseCommand): DATA_AGENTS = (AEAgent, GEOAgent, RNASeqAgent) DATA_SOURCE_NAMES = [agent.SOURCE_NAME for agent in DATA_AGENTS] - RE_ACCESSION = re.compile(r"(\D+)(\d+)") - RE_DATE = re.compile(r"\d{4}-\d{2}-\d{2}") # TODO(ark): remove after upgrade to python3.8 where parser argument # "extend" action is directly available. @@ -136,11 +134,12 @@ def validate_args(self, options) -> None: """Validates arguments.""" errors = list() + RE_DATE = re.compile(r"\d{4}-\d{2}-\d{2}") since = options["since"] until = options["until"] - if not self.RE_DATE.match(since): + if not RE_DATE.match(since): errors.append('The -s, --since value must match "YYYY-MM-DD" format.') - if until and not self.RE_DATE.match(until): + if until and not RE_DATE.match(until): errors.append('The -u, --until value must match "YYYY-MM-DD" format.') if since and until and since > until: errors.append("The -s, --since date must be earlier than -u, --until date.") @@ -213,11 +212,12 @@ def handle(self, *args, **options): f"new accession{pluralize(agent_accessions_count)}." ) + RE_ACCESSION = re.compile(r"(\D+)(\d+)") gathered_accessions = sorted( # Sort the resulting list. - (ga for ga in gathered_accessions if self.RE_ACCESSION.match(ga.accession_code)), + (ga for ga in gathered_accessions if RE_ACCESSION.match(ga.accession_code)), key=lambda ga: ( - self.RE_ACCESSION.match(ga.accession_code).group(1), - int(self.RE_ACCESSION.match(ga.accession_code).group(2)), + RE_ACCESSION.match(ga.accession_code).group(1), + int(RE_ACCESSION.match(ga.accession_code).group(2)), ), ) # Limit the number of output entries. From ea640a01703075ce4e9f5bc99f7acb797f80541d Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 5 Apr 2023 11:46:39 -0700 Subject: [PATCH 093/220] Add batch processing. --- .../management/commands/gather_accessions.py | 13 +++++++++---- 1 file changed, 9 insertions(+), 4 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 3c1ac8f73..6cf1e4873 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -239,14 +239,19 @@ def handle(self, *args, **options): output = "\n".join((str(ga) for ga in gathered_accessions)) else: output = "No new accessions gathered." - print(output) - else: + exit(output) + + BATCH_SIZE = 100 + for batch_start in range(0, len(gathered_accessions), BATCH_SIZE): + batch = gathered_accessions[batch_start : batch_start + BATCH_SIZE] try: - GatheredAccession.objects.bulk_create(gathered_accessions) - added_accessions_count = len(gathered_accessions) + GatheredAccession.objects.bulk_create(batch) + added_accessions_count = len(batch) logger.info( f"{added_accessions_count} new accession{pluralize(added_accessions_count)} " f"h{pluralize(added_accessions_count, 'as,ave')} been added to the database. " ) + logger.info("Added accessions: '%s'" % batch) except IntegrityError as e: logger.exception(f"Could not save new accessions to the database: {e}") + logger.info("Failed accessions: '%s'" % batch) From 09e650edffeac5cc9bc058b6b038fc6cee0dcac3 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 5 Apr 2023 12:44:30 -0700 Subject: [PATCH 094/220] Address review comments. --- .../management/commands/gather_accessions.py | 15 +++++++++++---- 1 file changed, 11 insertions(+), 4 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 6cf1e4873..ce8333781 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -25,7 +25,8 @@ class Command(BaseCommand): """Creates agents and runs actual accession gathering.""" - DATA_AGENTS = (AEAgent, GEOAgent, RNASeqAgent) + DATA_AGENTS = (AEAgent, GEOAgent) + # DATA_AGENTS = (AEAgent, GEOAgent, RNASeqAgent) DATA_SOURCE_NAMES = [agent.SOURCE_NAME for agent in DATA_AGENTS] # TODO(ark): remove after upgrade to python3.8 where parser argument @@ -52,7 +53,12 @@ def add_arguments(self, parser) -> None: type=str, help="Path to a file containing ArrayExpress ID(s) to use for filtering.", ) - parser.add_argument("-c", "--count", type=int, help="Number of accessions to collect.") + parser.add_argument( + "-c", + "--count", + type=int, + help="Number of accessions to collect.", + ) parser.add_argument( "-d", "--dry-run", @@ -244,6 +250,7 @@ def handle(self, *args, **options): BATCH_SIZE = 100 for batch_start in range(0, len(gathered_accessions), BATCH_SIZE): batch = gathered_accessions[batch_start : batch_start + BATCH_SIZE] + batch_accessions = (ga.accession_code for ga in batch) try: GatheredAccession.objects.bulk_create(batch) added_accessions_count = len(batch) @@ -251,7 +258,7 @@ def handle(self, *args, **options): f"{added_accessions_count} new accession{pluralize(added_accessions_count)} " f"h{pluralize(added_accessions_count, 'as,ave')} been added to the database. " ) - logger.info("Added accessions: '%s'" % batch) + logger.info("Added accessions: %s." % ", ".join(batch_accessions)) except IntegrityError as e: logger.exception(f"Could not save new accessions to the database: {e}") - logger.info("Failed accessions: '%s'" % batch) + logger.info("Failed accessions: %s." % ", ".join(batch_accessions)) From a26b2b89922254aed03ca1033cacd9d50ee1c221 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 5 Apr 2023 15:16:10 -0700 Subject: [PATCH 095/220] Address review comments. --- .../management/commands/automatic_survey.py | 69 ++++++++++--------- 1 file changed, 35 insertions(+), 34 deletions(-) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py b/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py index 6dc1273ac..be1d8132a 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/automatic_survey.py @@ -37,29 +37,29 @@ def create_survey_jobs(days, quota): remaining_quota = get_remaining_quota(days, quota) if not remaining_quota: - return - - gathered_accessions = [ - ga["accession_code"] - for ga in GatheredAccession.objects.order_by("created_at").values("accession_code") - ] - - batch_start = 0 - batch_size = 1000 - queued_accessions = list() - while True: - batch_accessions = gathered_accessions[batch_start : batch_start + batch_size] - if not batch_accessions: + logger.info("Unable to create new survey jobs: remaining quota is 0.") + return None + + gathered_accessions = GatheredAccession.objects.order_by("created_at").values_list( + "accession_code", flat=True + ) + + BATCH_SIZE = 100 + queued_accessions = [] + for batch_start in range(0, len(gathered_accessions), BATCH_SIZE): + if len(queued_accessions) == remaining_quota: + logger.info("Unable to create new survey jobs: remaining quota is 0.") break - surveyed_accessions = ( - sa["accession_code"] - for sa in SurveyedAccession.objects.filter(accession_code__in=batch_accessions).values( - "accession_code" - ) - ) + batch = gathered_accessions[batch_start : batch_start + BATCH_SIZE] + if not batch: + break + + surveyed_accessions = SurveyedAccession.objects.filter( + accession_code__in=batch + ).values_list("accession_code", flat=True) - for accession_code in set(batch_accessions) - set(surveyed_accessions): + for accession_code in set(batch).difference(set(surveyed_accessions)): try: queue_surveyor_for_accession(accession_code) except Exception as e: @@ -69,20 +69,21 @@ def create_survey_jobs(days, quota): if len(queued_accessions) == remaining_quota: break - if len(queued_accessions) == remaining_quota: - break - - batch_start += batch_size - - queued_accession_count = len(queued_accessions) - logger.info( - f"Queued {queued_accession_count} accession{pluralize(queued_accession_count)} " - f"based on {quota} accession{pluralize(quota)} per {days} " - f"day{pluralize(days)} quota." - ) - SurveyedAccession.objects.bulk_create( - (SurveyedAccession(accession_code=accession_code) for accession_code in queued_accessions) - ) + try: + SurveyedAccession.objects.bulk_create( + ( + SurveyedAccession(accession_code=accession_code) + for accession_code in queued_accessions + ) + ) + queued_accession_count = len(queued_accessions) + logger.info( + f"Queued {queued_accession_count} accession{pluralize(queued_accession_count)} " + f"based on {quota} accession{pluralize(quota)} per {days} " + f"day{pluralize(days)} quota." + ) + except Exception as e: + logger.error("Couldn't add surveyed accessions due to: %s" % e) def get_remaining_quota(days, quota): From c4176bc28213d35d0644fbff97518115fdabdad7 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 6 Apr 2023 10:52:47 -0700 Subject: [PATCH 096/220] Add GEOMetaDB support. For now it's going to be a part of Foreman Docker image. Migrate to dynamically loaded data later. --- foreman/data_refinery_foreman/gatherer/agents/geo_agent.py | 2 +- .../gatherer/management/commands/gather_accessions.py | 7 +++---- foreman/dockerfiles/Dockerfile.foreman | 4 ++++ 3 files changed, 8 insertions(+), 5 deletions(-) diff --git a/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py b/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py index 22a3bd0bd..7f59306fe 100644 --- a/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py +++ b/foreman/data_refinery_foreman/gatherer/agents/geo_agent.py @@ -25,7 +25,7 @@ class GEOAgent(AccessionAgentBase): # TODO(ark): move the DB file from Docker image to S3. # Implement syncing procedure. # Update URL once the original file is available again. - DB_PATH = "data/microarray/GEOmetadb.sqlite" + DB_PATH = "/home/user/data/microarray/GEOmetadb.sqlite" SOURCE = "geo-meta-db" SOURCE_NAME = "microarray-geo" TECHNOLOGY = "microarray" diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index ce8333781..07d708007 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -25,8 +25,7 @@ class Command(BaseCommand): """Creates agents and runs actual accession gathering.""" - DATA_AGENTS = (AEAgent, GEOAgent) - # DATA_AGENTS = (AEAgent, GEOAgent, RNASeqAgent) + DATA_AGENTS = (AEAgent, GEOAgent, RNASeqAgent) DATA_SOURCE_NAMES = [agent.SOURCE_NAME for agent in DATA_AGENTS] # TODO(ark): remove after upgrade to python3.8 where parser argument @@ -214,8 +213,8 @@ def handle(self, *args, **options): gathered_accessions.update(agent_accessions) logger.info( - f"Since {options['since']} {agent} has gathered {agent_accessions_count} " - f"new accession{pluralize(agent_accessions_count)}." + f"{agent} has gathered {agent_accessions_count} " + f"new accession{pluralize(agent_accessions_count)} since {options['since']}." ) RE_ACCESSION = re.compile(r"(\D+)(\d+)") diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index f1781ec0a..ec0ce50db 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -5,6 +5,10 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user +ADD https://gbnci.cancer.gov/geo/GEOmetadb.sqlite.gz data/microarray/GEOmetadb.sqlite.gz +RUN gunzip data/microarray/GEOmetadb.sqlite.gz && \ + chmod 644 data/microarray/GEOmetadb.sqlite + COPY foreman/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt From 45b3ab12d45d8741dd7e36ebab563983c1b89102 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 6 Apr 2023 14:01:38 -0700 Subject: [PATCH 097/220] Update foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py Co-authored-by: David --- .../gatherer/management/commands/gather_accessions.py | 1 + 1 file changed, 1 insertion(+) diff --git a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py index 07d708007..97dbc45d1 100644 --- a/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py +++ b/foreman/data_refinery_foreman/gatherer/management/commands/gather_accessions.py @@ -217,6 +217,7 @@ def handle(self, *args, **options): f"new accession{pluralize(agent_accessions_count)} since {options['since']}." ) + # Regex to split into two groups, non-digits and digits ex: (GSE)(1234). RE_ACCESSION = re.compile(r"(\D+)(\d+)") gathered_accessions = sorted( # Sort the resulting list. (ga for ga in gathered_accessions if RE_ACCESSION.match(ga.accession_code)), From 78dc4e78d8a4387df31bffc304562ca00ff59dd8 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 6 Apr 2023 14:03:48 -0700 Subject: [PATCH 098/220] Fix indentation. --- foreman/dockerfiles/Dockerfile.foreman | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index ec0ce50db..94e5c185a 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -7,7 +7,7 @@ WORKDIR /home/user ADD https://gbnci.cancer.gov/geo/GEOmetadb.sqlite.gz data/microarray/GEOmetadb.sqlite.gz RUN gunzip data/microarray/GEOmetadb.sqlite.gz && \ - chmod 644 data/microarray/GEOmetadb.sqlite + chmod 644 data/microarray/GEOmetadb.sqlite COPY foreman/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt From 04e3919d7ade01eb4f01b0ca5905f5e9550e52a9 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 6 Apr 2023 14:07:40 -0700 Subject: [PATCH 099/220] Update workers/keys/README.md Co-authored-by: David --- workers/keys/README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workers/keys/README.md b/workers/keys/README.md index bf95182d9..153247aa1 100644 --- a/workers/keys/README.md +++ b/workers/keys/README.md @@ -1,3 +1,3 @@ `asperaweb_id_dsa.openssh` is a publicly available "private" key required by ascp. -See https://www.ibm.com/docs/en/aspera-on-cloud?topic=ak-fasp-security-aspera-connect-transfer-client-authorized-keys-aspshell +See [IBM's documentation](https://www.ibm.com/docs/en/aspera-on-cloud?topic=ak-fasp-security-aspera-connect-transfer-client-authorized-keys-aspshell) for more details. From a34004e2d555076f2def5d9b3259e84e6f6be429 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 11 Apr 2023 10:53:54 -0700 Subject: [PATCH 100/220] Fix common/oreman tests. Migrate common/migrations image to `dr_base`. Move foreman tests to tests directory. Add separate settings file (tests/settings.py). Refactor import according to the new structure. --- common/R/dependencies/common_tests/renv.R | 40 +- common/R/dependencies/common_tests/renv.lock | 501 ++++++------------ common/dockerfiles/Dockerfile.migrations | 23 +- .../commands/update_experiment_metadata.py | 13 +- .../surveyor/array_express.py | 297 ++++++----- foreman/run_tests.sh | 1 + foreman/tests/__init__.py | 0 foreman/tests/foreman/__init__.py | 0 foreman/tests/foreman/management/__init__.py | 0 .../foreman/management/commands/__init__.py | 0 .../commands/test_correct_affy_cdfs.py | 0 .../commands/test_create_compendia.py | 0 .../test_create_missing_downloader_jobs.py | 6 - .../test_create_missing_processor_jobs.py | 5 - .../commands/test_create_quantpendia.py | 0 .../test_import_external_sample_attributes.py | 0 .../test_import_external_sample_keywords.py | 0 .../commands/test_organism_shepherd.py | 0 .../commands/test_rerun_salmon_old_samples.py | 0 .../management/commands/test_retry_samples.py | 9 - .../management/commands/test_run_tximport.py | 0 .../test_update_experiment_metadata.py | 59 +-- .../foreman/test_downloader_job_manager.py | 3 +- .../foreman/test_end_to_end.py | 0 .../foreman/test_job_control.py | 0 .../foreman/test_job_requeuing.py | 7 +- .../foreman/test_processor_job_manager.py | 3 +- .../foreman/test_survey_job_manager.py | 3 +- .../test_utils.py => tests/foreman/utils.py} | 0 foreman/tests/settings.py | 5 + foreman/tests/surveyor/management/__init__.py | 0 .../surveyor/management/commands/__init__.py | 0 .../commands/test_dispatch_qn_jobs.py | 0 .../management/commands/test_unsurvey.py | 0 .../surveyor/test_array_express.py | 0 .../surveyor/test_external_source.py | 0 .../surveyor/test_geo.py | 0 .../surveyor/test_harmony.py | 0 .../surveyor/test_sra.py | 0 .../surveyor/test_surveyor.py | 0 .../surveyor/test_transcriptome_index.py | 0 .../test_imports.py | 56 +- .../test_runner.py | 0 .../R/dependencies/install_devtools_renv.R | 4 +- workers/R/renv_load.R | 2 +- workers/R/renv_save.R | 2 +- .../processors/requirements.in | 12 +- .../processors/requirements.txt | 167 +++--- workers/run_tests.sh | 3 +- workers/tests/processors/test_compendia.py | 2 +- workers/tests/processors/test_illumina.py | 4 +- 51 files changed, 517 insertions(+), 710 deletions(-) create mode 100644 foreman/tests/__init__.py create mode 100644 foreman/tests/foreman/__init__.py create mode 100644 foreman/tests/foreman/management/__init__.py create mode 100644 foreman/tests/foreman/management/commands/__init__.py rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_correct_affy_cdfs.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_create_compendia.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_create_missing_downloader_jobs.py (96%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_create_missing_processor_jobs.py (99%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_create_quantpendia.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_import_external_sample_attributes.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_import_external_sample_keywords.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_organism_shepherd.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_rerun_salmon_old_samples.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_retry_samples.py (92%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_run_tximport.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/management/commands/test_update_experiment_metadata.py (74%) rename foreman/{data_refinery_foreman => tests}/foreman/test_downloader_job_manager.py (99%) rename foreman/{data_refinery_foreman => tests}/foreman/test_end_to_end.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/test_job_control.py (100%) rename foreman/{data_refinery_foreman => tests}/foreman/test_job_requeuing.py (97%) rename foreman/{data_refinery_foreman => tests}/foreman/test_processor_job_manager.py (99%) rename foreman/{data_refinery_foreman => tests}/foreman/test_survey_job_manager.py (99%) rename foreman/{data_refinery_foreman/foreman/test_utils.py => tests/foreman/utils.py} (100%) create mode 100644 foreman/tests/settings.py create mode 100644 foreman/tests/surveyor/management/__init__.py create mode 100644 foreman/tests/surveyor/management/commands/__init__.py rename foreman/{data_refinery_foreman => tests}/surveyor/management/commands/test_dispatch_qn_jobs.py (100%) rename foreman/{data_refinery_foreman => tests}/surveyor/management/commands/test_unsurvey.py (100%) rename foreman/{data_refinery_foreman => tests}/surveyor/test_array_express.py (100%) rename foreman/{data_refinery_foreman => tests}/surveyor/test_external_source.py (100%) rename foreman/{data_refinery_foreman => tests}/surveyor/test_geo.py (100%) rename foreman/{data_refinery_foreman => tests}/surveyor/test_harmony.py (100%) rename foreman/{data_refinery_foreman => tests}/surveyor/test_sra.py (100%) rename foreman/{data_refinery_foreman => tests}/surveyor/test_surveyor.py (100%) rename foreman/{data_refinery_foreman => tests}/surveyor/test_transcriptome_index.py (100%) rename foreman/{data_refinery_foreman => tests}/test_imports.py (53%) rename foreman/{data_refinery_foreman => tests}/test_runner.py (100%) diff --git a/common/R/dependencies/common_tests/renv.R b/common/R/dependencies/common_tests/renv.R index b5a23e262..019586a8c 100644 --- a/common/R/dependencies/common_tests/renv.R +++ b/common/R/dependencies/common_tests/renv.R @@ -1,24 +1,20 @@ -renv::install("rlang@1.0.4") -renv::install("dplyr@1.0.0") -renv::install("tidyr@1.1.0") -renv::install("ff@2.2-14") -renv::install("locfit@1.5-9.4") -renv::install("cli@3.3.0") -renv::install("lifecycle@1.0.3") -renv::install("vctrs@0.4.1") - renv::install( - "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/GEOquery_2.56.0.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/SCAN.UPC_2.30.0.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/affy_1.66.0.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/genefilter_1.70.0.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/sva_3.36.0.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/tximport_1.16.1.tar.gz", - "https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz" + "affy", + "affyio", + "AnnotationDbi", + "dplyr@1.0.2", + "ff@2.2-14", + "genefilter", + "GEOquery", + "limma", + "oligo", + "oligoClasses", + "preprocessCore", + "purrr@0.3.5", + "readr@1.3.1", + "SCAN.UPC", + "sva", + "tidyr@1.1.0", + "tximport", + "zlibbioc" ) diff --git a/common/R/dependencies/common_tests/renv.lock b/common/R/dependencies/common_tests/renv.lock index a715ce7d2..5c656dab7 100644 --- a/common/R/dependencies/common_tests/renv.lock +++ b/common/R/dependencies/common_tests/renv.lock @@ -1,18 +1,18 @@ { "R": { - "Version": "3.6.3", + "Version": "3.4.4", "Repositories": [ { "Name": "Bioconductor", - "URL": "https://bioconductor.org/packages/3.11/bioc" + "URL": "https://bioconductor.org/packages/3.6/bioc" }, { "Name": "BioconductorAnnotation", - "URL": "https://bioconductor.org/packages/3.11/data/annotation" + "URL": "https://bioconductor.org/packages/3.6/data/annotation" }, { "Name": "BioconductorExperiment", - "URL": "https://bioconductor.org/packages/3.11/data/experiment" + "URL": "https://bioconductor.org/packages/3.6/data/experiment" }, { "Name": "CRAN", @@ -23,15 +23,9 @@ "Packages": { "AnnotationDbi": { "Package": "AnnotationDbi", - "Version": "1.50.3", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/AnnotationDbi_1.50.3.tar.gz", - "git_url": "https://git.bioconductor.org/packages/AnnotationDbi", - "git_branch": "RELEASE_3_11", - "git_last_commit": "efa119c", - "git_last_commit_date": "2020-07-24", - "Hash": "8118f8655ad5604099a0e8ac14090f91", + "Version": "1.40.0", + "Source": "Bioconductor", + "Hash": "abb20d74ae5ea08a8d9616a6f1bbaca5", "Requirements": [ "Biobase", "BiocGenerics", @@ -51,45 +45,32 @@ }, "Biobase": { "Package": "Biobase", - "Version": "2.48.0", + "Version": "2.38.0", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/Biobase", - "git_branch": "RELEASE_3_11", - "git_last_commit": "4ffadf2", - "git_last_commit_date": "2020-04-27", - "Hash": "da9b1bc36fb35ebbec219dc007657f21", + "Hash": "8c8060a3f339c35f1dc14af3b7d22d71", "Requirements": [ "BiocGenerics" ] }, "BiocGenerics": { "Package": "BiocGenerics", - "Version": "0.34.0", + "Version": "0.24.0", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/BiocGenerics", - "git_branch": "RELEASE_3_11", - "git_last_commit": "f7c2020", - "git_last_commit_date": "2020-04-27", - "Hash": "ae191b5608f175de0f69b6302390c705", + "Hash": "108ff3e3233e79aef825decc9fa87ee3", "Requirements": [] }, - "BiocManager": { - "Package": "BiocManager", - "Version": "1.30.19", - "Source": "Repository", - "Repository": "CRAN", - "Hash": "726b3bc83ff8e0c35c7efdb74a462b0d", + "BiocInstaller": { + "Package": "BiocInstaller", + "Version": "1.28.0", + "Source": "Bioconductor", + "Hash": "ee02868b8bd3657a3463d8bc3a64d831", "Requirements": [] }, "BiocParallel": { "Package": "BiocParallel", - "Version": "1.22.0", + "Version": "1.12.0", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/BiocParallel", - "git_branch": "RELEASE_3_11", - "git_last_commit": "430511c", - "git_last_commit_date": "2020-04-27", - "Hash": "6457b5a769de3e02704fd1b529a3c6e1", + "Hash": "4fc0d93ea840783e2000f15f111b8a9b", "Requirements": [ "BH", "futile.logger", @@ -98,19 +79,14 @@ }, "Biostrings": { "Package": "Biostrings", - "Version": "2.56.0", + "Version": "2.46.0", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/Biostrings", - "git_branch": "RELEASE_3_11", - "git_last_commit": "79b7ec8", - "git_last_commit_date": "2020-04-27", - "Hash": "f5d135c565a244740559409e48c410cd", + "Hash": "12005b8dd90f7f7f9864ee9d6965c366", "Requirements": [ "BiocGenerics", "IRanges", "S4Vectors", - "XVector", - "crayon" + "XVector" ] }, "DBI": { @@ -138,32 +114,21 @@ }, "DelayedArray": { "Package": "DelayedArray", - "Version": "0.14.1", + "Version": "0.4.1", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/DelayedArray", - "git_branch": "RELEASE_3_11", - "git_last_commit": "52230a4", - "git_last_commit_date": "2020-07-13", - "Hash": "8430a6ec606d0d92198ff107f1d98261", + "Hash": "c2572a27fb1281ee4c11390826f326a1", "Requirements": [ "BiocGenerics", "IRanges", - "Matrix", "S4Vectors", "matrixStats" ] }, "GEOquery": { "Package": "GEOquery", - "Version": "2.56.0", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/GEOquery_2.56.0.tar.gz", - "git_url": "https://git.bioconductor.org/packages/GEOquery", - "git_branch": "RELEASE_3_11", - "git_last_commit": "50417b3", - "git_last_commit_date": "2020-04-27", - "Hash": "4f5f180eb5db85e0741db59fee6158c2", + "Version": "2.46.15", + "Source": "Bioconductor", + "Hash": "71fbb4c39d7ea4ca06dcfdfd41ef1ee5", "Requirements": [ "Biobase", "dplyr", @@ -177,13 +142,9 @@ }, "GenomeInfoDb": { "Package": "GenomeInfoDb", - "Version": "1.24.2", + "Version": "1.14.0", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/GenomeInfoDb", - "git_branch": "RELEASE_3_11", - "git_last_commit": "e3713f3", - "git_last_commit_date": "2020-06-14", - "Hash": "f1556568ab93fd7704380539fca71e6a", + "Hash": "39dc4261100e402165a6db62f364b493", "Requirements": [ "BiocGenerics", "GenomeInfoDbData", @@ -194,20 +155,16 @@ }, "GenomeInfoDbData": { "Package": "GenomeInfoDbData", - "Version": "1.2.3", + "Version": "1.0.0", "Source": "Bioconductor", - "Hash": "8fe62eac6e27b8e56d51f9ae6030cbcd", + "Hash": "336cc20fd69db19ebfad8a73e05d88b3", "Requirements": [] }, "GenomicRanges": { "Package": "GenomicRanges", - "Version": "1.40.0", + "Version": "1.30.3", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/GenomicRanges", - "git_branch": "RELEASE_3_11", - "git_last_commit": "a9e18e4", - "git_last_commit_date": "2020-04-27", - "Hash": "05cd2ba8e1e373e9089454cf762df723", + "Hash": "ecfab21e609cea7ee297e0e1230b5bb4", "Requirements": [ "BiocGenerics", "GenomeInfoDb", @@ -218,13 +175,9 @@ }, "IRanges": { "Package": "IRanges", - "Version": "2.22.2", + "Version": "2.12.0", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/IRanges", - "git_branch": "RELEASE_3_11", - "git_last_commit": "8c5e991", - "git_last_commit_date": "2020-05-20", - "Hash": "1403fa5c98c5f48b989a6bc5a65c6c25", + "Hash": "d89aecf720b113b47f936c48b356d264", "Requirements": [ "BiocGenerics", "S4Vectors" @@ -232,26 +185,26 @@ }, "KernSmooth": { "Package": "KernSmooth", - "Version": "2.23-20", + "Version": "2.23-15", "Source": "Repository", "Repository": "CRAN", - "Hash": "8dcfa99b14c296bc9f1fd64d52fd3ce7", + "Hash": "081f417f4d6d55b7e8981433e8404a22", "Requirements": [] }, "MASS": { "Package": "MASS", - "Version": "7.3-58", + "Version": "7.3-49", "Source": "Repository", "Repository": "CRAN", - "Hash": "4bf2c61e656bbc238634c798c208d386", + "Hash": "f47828414f155725f342d93be7fcfabd", "Requirements": [] }, "Matrix": { "Package": "Matrix", - "Version": "1.5-1", + "Version": "1.2-12", "Source": "Repository", "Repository": "CRAN", - "Hash": "539dc0c0c05636812f1080f473d2c177", + "Hash": "2451d6490ec017b18b6eff460c65fd85", "Requirements": [ "lattice" ] @@ -266,25 +219,25 @@ }, "RCurl": { "Package": "RCurl", - "Version": "1.98-1.9", + "Version": "1.98-1.10", "Source": "Repository", "Repository": "CRAN", - "Hash": "ea2f19dade6bd911b29b9bbb115d08f6", + "Hash": "35136c52e39f2679ebbe7bf448e3bd5a", "Requirements": [ "bitops" ] }, "RSQLite": { "Package": "RSQLite", - "Version": "2.2.20", + "Version": "2.3.0", "Source": "Repository", "Repository": "CRAN", - "Hash": "b5df65015a2d74c144a1419dab6ffd3b", + "Hash": "264c1861d7ac1dc71ecb5ac5c4c1318a", "Requirements": [ "DBI", - "Rcpp", "bit64", "blob", + "cpp11", "memoise", "pkgconfig", "plogr" @@ -292,36 +245,26 @@ }, "Rcpp": { "Package": "Rcpp", - "Version": "1.0.10", + "Version": "1.0.7", "Source": "Repository", "Repository": "CRAN", - "Hash": "e749cae40fa9ef469b6050959517453c", + "Hash": "dab19adae4440ae55aa8a9d238b246bb", "Requirements": [] }, "S4Vectors": { "Package": "S4Vectors", - "Version": "0.26.1", + "Version": "0.16.0", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/S4Vectors", - "git_branch": "RELEASE_3_11", - "git_last_commit": "935769c", - "git_last_commit_date": "2020-05-15", - "Hash": "c252d04c13a3a6667d3cc140360780f1", + "Hash": "e9cd4cea8c93cbdd8595e562c8ed0624", "Requirements": [ "BiocGenerics" ] }, "SCAN.UPC": { "Package": "SCAN.UPC", - "Version": "2.30.0", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/SCAN.UPC_2.30.0.tar.gz", - "git_url": "https://git.bioconductor.org/packages/SCAN.UPC", - "git_branch": "RELEASE_3_11", - "git_last_commit": "052d3c4", - "git_last_commit_date": "2020-04-27", - "Hash": "f76f2473345a4c25263772c3c862df0e", + "Version": "2.20.0", + "Source": "Bioconductor", + "Hash": "1583bdf3637b41eb5f8812e7ac678f73", "Requirements": [ "Biobase", "Biostrings", @@ -337,13 +280,9 @@ }, "SummarizedExperiment": { "Package": "SummarizedExperiment", - "Version": "1.18.2", + "Version": "1.8.1", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/SummarizedExperiment", - "git_branch": "RELEASE_3_11", - "git_last_commit": "e22fafe", - "git_last_commit_date": "2020-07-09", - "Hash": "811eb16b3d542aa098d4c89f0985f3bc", + "Hash": "57080b28b2f5606100e02a7ab01b3022", "Requirements": [ "Biobase", "BiocGenerics", @@ -365,13 +304,9 @@ }, "XVector": { "Package": "XVector", - "Version": "0.28.0", + "Version": "0.18.0", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/XVector", - "git_branch": "RELEASE_3_11", - "git_last_commit": "8bda737", - "git_last_commit_date": "2020-04-27", - "Hash": "a5669a9d44010120a5c10be7977f0626", + "Hash": "c677dd00d99bd1f81caf0fc3d9fa7672", "Requirements": [ "BiocGenerics", "IRanges", @@ -381,30 +316,20 @@ }, "affxparser": { "Package": "affxparser", - "Version": "1.60.0", + "Version": "1.50.0", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/affxparser", - "git_branch": "RELEASE_3_11", - "git_last_commit": "22b10b1", - "git_last_commit_date": "2020-04-27", - "Hash": "4fca40e4bb842b56d6be95bff88ac85f", + "Hash": "86914cfc7998f083d207a8ae84488697", "Requirements": [] }, "affy": { "Package": "affy", - "Version": "1.66.0", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/affy_1.66.0.tar.gz", - "git_url": "https://git.bioconductor.org/packages/affy", - "git_branch": "RELEASE_3_11", - "git_last_commit": "ae131f8", - "git_last_commit_date": "2020-04-27", - "Hash": "7d4686f9fa0ba9db6d0f596058dd1a15", + "Version": "1.56.0", + "Source": "Bioconductor", + "Hash": "1022ecaff1da7aaa5f1ebe6168794e97", "Requirements": [ "Biobase", "BiocGenerics", - "BiocManager", + "BiocInstaller", "affyio", "preprocessCore", "zlibbioc" @@ -412,28 +337,18 @@ }, "affyio": { "Package": "affyio", - "Version": "1.58.0", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/affyio_1.58.0.tar.gz", - "git_url": "https://git.bioconductor.org/packages/affyio", - "git_branch": "RELEASE_3_11", - "git_last_commit": "76fee54", - "git_last_commit_date": "2020-04-27", - "Hash": "841239ddcf5f028105cd3687976948ef", + "Version": "1.48.0", + "Source": "Bioconductor", + "Hash": "f973e1ee016e8ff7095847e0769a9d97", "Requirements": [ "zlibbioc" ] }, "annotate": { "Package": "annotate", - "Version": "1.66.0", + "Version": "1.56.2", "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/annotate", - "git_branch": "RELEASE_3_11", - "git_last_commit": "a63378f", - "git_last_commit_date": "2020-04-27", - "Hash": "61677ca20165e7790955cdd1fc27281e", + "Hash": "064f14d8325abc226f7433f9ff8094c9", "Requirements": [ "AnnotationDbi", "Biobase", @@ -517,10 +432,10 @@ }, "boot": { "Package": "boot", - "Version": "1.3-28", + "Version": "1.3-20", "Source": "Repository", "Repository": "CRAN", - "Hash": "0baa960e3b49c6176a4f42addcbacc59", + "Hash": "560a6eb3b4658e2ce3d0f8feb087fe56", "Requirements": [] }, "brew": { @@ -531,6 +446,17 @@ "Hash": "92a5f887f9ae3035ac7afde22ba73ee9", "Requirements": [] }, + "cachem": { + "Package": "cachem", + "Version": "1.0.7", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "cda74447c42f529de601fe4d4050daef", + "Requirements": [ + "fastmap", + "rlang" + ] + }, "callr": { "Package": "callr", "Version": "3.4.3", @@ -544,10 +470,10 @@ }, "class": { "Package": "class", - "Version": "7.3-20", + "Version": "7.3-14", "Source": "Repository", "Repository": "CRAN", - "Hash": "da09d82223e669d270e47ed24ac8686e", + "Hash": "c39d47020cdf42e88bd80c83ca6a0a0b", "Requirements": [ "MASS" ] @@ -570,18 +496,18 @@ }, "cluster": { "Package": "cluster", - "Version": "2.1.4", + "Version": "2.0.6", "Source": "Repository", "Repository": "CRAN", - "Hash": "5edbbabab6ce0bf7900a74fd4358628e", + "Hash": "ea8c392e186024710ab52bc405212f5e", "Requirements": [] }, "codetools": { "Package": "codetools", - "Version": "0.2-18", + "Version": "0.2-15", "Source": "Repository", "Repository": "CRAN", - "Hash": "019388fc48e48b3da0d3a76ff94608a8", + "Hash": "9f88e0b246d1b22d1a88b9aa4ea57ebd", "Requirements": [] }, "commonmark": { @@ -698,10 +624,10 @@ }, "dplyr": { "Package": "dplyr", - "Version": "1.0.0", + "Version": "1.0.2", "Source": "Repository", "Repository": "CRAN", - "Hash": "4011f62581a34080e44105d4aa05a97f", + "Hash": "d0509913b27ea898189ee664b6030dc2", "Requirements": [ "R6", "ellipsis", @@ -715,21 +641,6 @@ "vctrs" ] }, - "edgeR": { - "Package": "edgeR", - "Version": "3.30.3", - "Source": "Bioconductor", - "git_url": "https://git.bioconductor.org/packages/edgeR", - "git_branch": "RELEASE_3_11", - "git_last_commit": "7a3a272", - "git_last_commit_date": "2020-06-02", - "Hash": "fed69054bac345df0b60fc9afa7101e9", - "Requirements": [ - "Rcpp", - "limma", - "locfit" - ] - }, "ellipsis": { "Package": "ellipsis", "Version": "0.3.2", @@ -756,6 +667,14 @@ "Hash": "7fce217eaaf8016e72065e85c73027b5", "Requirements": [] }, + "fastmap": { + "Package": "fastmap", + "Version": "1.1.1", + "Source": "Repository", + "Repository": "CRAN", + "Hash": "f7736a18de97dea803bde0a2daaafb27", + "Requirements": [] + }, "ff": { "Package": "ff", "Version": "2.2-14", @@ -779,10 +698,10 @@ }, "foreign": { "Package": "foreign", - "Version": "0.8-76", + "Version": "0.8-69", "Source": "Repository", "Repository": "CRAN", - "Hash": "649b9c7a979d50d869578c73fed072cd", + "Hash": "6f953f3e73fc00af8cdad0864f535f97", "Requirements": [] }, "formatR": { @@ -822,19 +741,13 @@ }, "genefilter": { "Package": "genefilter", - "Version": "1.70.0", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/genefilter_1.70.0.tar.gz", - "git_url": "https://git.bioconductor.org/packages/genefilter", - "git_branch": "RELEASE_3_11", - "git_last_commit": "a071480", - "git_last_commit_date": "2020-04-27", - "Hash": "8db2ebb178f95b85a77813e2d99c6b40", + "Version": "1.60.0", + "Source": "Bioconductor", + "Hash": "e325ea00e99e1c3ce474aa07b88cd81c", "Requirements": [ "AnnotationDbi", "Biobase", - "BiocGenerics", + "S4Vectors", "annotate", "survival" ] @@ -870,10 +783,10 @@ }, "glue": { "Package": "glue", - "Version": "1.6.2", + "Version": "1.4.1", "Source": "Repository", "Repository": "CRAN", - "Hash": "4f2596dfb05dac67b9dc558e5c6fba2e", + "Hash": "f43e0d5e85ccb0a4045670c0607ee504", "Requirements": [] }, "highr": { @@ -900,13 +813,15 @@ }, "htmltools": { "Package": "htmltools", - "Version": "0.5.0", + "Version": "0.5.4", "Source": "Repository", "Repository": "CRAN", - "Hash": "7d651b7131794fe007b1ad6f21aaa401", + "Hash": "9d27e99cc90bd701c0a7a63e5923f9b7", "Requirements": [ "base64enc", "digest", + "ellipsis", + "fastmap", "rlang" ] }, @@ -999,10 +914,10 @@ }, "lattice": { "Package": "lattice", - "Version": "0.20-45", + "Version": "0.20-35", "Source": "Repository", "Repository": "CRAN", - "Hash": "b64cdbb2b340437c4ee047a1f4c4377b", + "Hash": "10a22a9a66fbe7944e9ef98985d7c927", "Requirements": [] }, "lazyeval": { @@ -1027,27 +942,11 @@ }, "limma": { "Package": "limma", - "Version": "3.44.3", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/limma_3.44.3.tar.gz", - "git_url": "https://git.bioconductor.org/packages/limma", - "git_branch": "RELEASE_3_11", - "git_last_commit": "90275de", - "git_last_commit_date": "2020-06-12", - "Hash": "6f85e30aa587815acc9551390ff0bbec", + "Version": "3.34.9", + "Source": "Bioconductor", + "Hash": "06c81c4043f41177428a83c73a59d094", "Requirements": [] }, - "locfit": { - "Package": "locfit", - "Version": "1.5-9.4", - "Source": "Repository", - "Repository": "CRAN", - "Hash": "760b5b542e8435237d1b3c253bfe18e7", - "Requirements": [ - "lattice" - ] - }, "magrittr": { "Package": "magrittr", "Version": "1.5", @@ -1077,20 +976,21 @@ }, "memoise": { "Package": "memoise", - "Version": "1.1.0", + "Version": "2.0.1", "Source": "Repository", "Repository": "CRAN", - "Hash": "58baa74e4603fcfb9a94401c58c8f9b1", + "Hash": "e2817ccf4a065c5d9d7f2cfbe7c1d78c", "Requirements": [ - "digest" + "cachem", + "rlang" ] }, "mgcv": { "Package": "mgcv", - "Version": "1.8-41", + "Version": "1.8-23", "Source": "Repository", "Repository": "CRAN", - "Hash": "6b3904f13346742caa3e82dd0303d4ad", + "Hash": "b1a3bba86e45ddea6b66e67623f587ee", "Requirements": [ "Matrix", "nlme" @@ -1106,33 +1006,27 @@ }, "nlme": { "Package": "nlme", - "Version": "3.1-161", + "Version": "3.1-131", "Source": "Repository", "Repository": "CRAN", - "Hash": "b49e163093c0ef46fbbfc40bcb87916c", + "Hash": "215a901b25abf4077a8e7d2d6ce2b211", "Requirements": [ "lattice" ] }, "nnet": { "Package": "nnet", - "Version": "7.3-18", + "Version": "7.3-12", "Source": "Repository", "Repository": "CRAN", - "Hash": "170da2130d5332bea7d6ede01875ba1d", + "Hash": "68287aec1f476c41d16ce1ace445800c", "Requirements": [] }, "oligo": { "Package": "oligo", - "Version": "1.52.1", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligo_1.52.1.tar.gz", - "git_url": "https://git.bioconductor.org/packages/oligo", - "git_branch": "RELEASE_3_11", - "git_last_commit": "cbd66c8", - "git_last_commit_date": "2020-08-10", - "Hash": "25a32b15cf0656f2624672150de9d750", + "Version": "1.42.0", + "Source": "Bioconductor", + "Hash": "694c134c6a0d42dd72084c9283735e1a", "Requirements": [ "Biobase", "BiocGenerics", @@ -1149,19 +1043,13 @@ }, "oligoClasses": { "Package": "oligoClasses", - "Version": "1.50.4", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/oligoClasses_1.50.4.tar.gz", - "git_url": "https://git.bioconductor.org/packages/oligoClasses", - "git_branch": "RELEASE_3_11", - "git_last_commit": "da7b856", - "git_last_commit_date": "2020-08-10", - "Hash": "f4190217bc7d1b697b37bd4711560084", + "Version": "1.40.0", + "Source": "Bioconductor", + "Hash": "c197dfefe8a4928044851fceb05b5eba", "Requirements": [ "Biobase", "BiocGenerics", - "BiocManager", + "BiocInstaller", "Biostrings", "DBI", "GenomicRanges", @@ -1176,10 +1064,10 @@ }, "openssl": { "Package": "openssl", - "Version": "1.4.2", + "Version": "2.0.3", "Source": "Repository", "Repository": "CRAN", - "Hash": "b3209c62052922b6c629544d94c8fa8a", + "Hash": "b9621e75c0652041002a19609fb23c5a", "Requirements": [ "askpass" ] @@ -1261,15 +1149,9 @@ }, "preprocessCore": { "Package": "preprocessCore", - "Version": "1.50.0", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/preprocessCore_1.50.0.tar.gz", - "git_url": "https://git.bioconductor.org/packages/preprocessCore", - "git_branch": "RELEASE_3_11", - "git_last_commit": "9230366", - "git_last_commit_date": "2020-04-27", - "Hash": "4d183aca4311a7610cb96d14e528e73e", + "Version": "1.40.0", + "Source": "Bioconductor", + "Hash": "db115919701b4e644b96de3f7523d17a", "Requirements": [] }, "prettyunits": { @@ -1291,19 +1173,6 @@ "ps" ] }, - "progress": { - "Package": "progress", - "Version": "1.2.2", - "Source": "Repository", - "Repository": "CRAN", - "Hash": "14dc9f7a3c91ebb14ec5bb9208a07061", - "Requirements": [ - "R6", - "crayon", - "hms", - "prettyunits" - ] - }, "promises": { "Package": "promises", "Version": "1.1.1", @@ -1328,10 +1197,10 @@ }, "purrr": { "Package": "purrr", - "Version": "0.3.4", + "Version": "0.3.5", "Source": "Repository", "Repository": "CRAN", - "Hash": "97def703420c8ab10d8f0e6c72101e02", + "Hash": "54842a2443c76267152eface28d9e90a", "Requirements": [ "magrittr", "rlang" @@ -1360,22 +1229,18 @@ }, "readr": { "Package": "readr", - "Version": "2.1.3", + "Version": "1.3.1", "Source": "Repository", "Repository": "CRAN", - "Hash": "2dfbfc673ccb3de3d8836b4b3bd23d14", + "Hash": "af8ab99cd936773a148963905736907b", "Requirements": [ + "BH", "R6", - "cli", + "Rcpp", "clipr", - "cpp11", "crayon", "hms", - "lifecycle", - "rlang", - "tibble", - "tzdb", - "vroom" + "tibble" ] }, "rematch2": { @@ -1446,10 +1311,10 @@ }, "rpart": { "Package": "rpart", - "Version": "4.1.19", + "Version": "4.1-13", "Source": "Repository", "Repository": "CRAN", - "Hash": "b3c892a81783376cc2204af0f5805a80", + "Hash": "6315535da80d5cc6c2e573966d8c8210", "Requirements": [] }, "rprojroot": { @@ -1530,28 +1395,21 @@ }, "survival": { "Package": "survival", - "Version": "3.4-0", + "Version": "2.41-3", "Source": "Repository", "Repository": "CRAN", - "Hash": "04411ae66ab4659230c067c32966fc20", + "Hash": "60b1da51f5e3556fd177170a1aa40b49", "Requirements": [ "Matrix" ] }, "sva": { "Package": "sva", - "Version": "3.36.0", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/sva_3.36.0.tar.gz", - "git_url": "https://git.bioconductor.org/packages/sva", - "git_branch": "RELEASE_3_11", - "git_last_commit": "d92b71f", - "git_last_commit_date": "2020-04-27", - "Hash": "7063ce04e0eeec513cff2d23b1eef5b1", + "Version": "3.26.0", + "Source": "Bioconductor", + "Hash": "c21e7fce3b904dae22f5d1a151b8a0c5", "Requirements": [ "BiocParallel", - "edgeR", "genefilter", "limma", "matrixStats", @@ -1643,27 +1501,11 @@ }, "tximport": { "Package": "tximport", - "Version": "1.16.1", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/tximport_1.16.1.tar.gz", - "git_url": "https://git.bioconductor.org/packages/tximport", - "git_branch": "RELEASE_3_11", - "git_last_commit": "a4fe5d3", - "git_last_commit_date": "2020-06-05", - "Hash": "f75925813c3e168c8d5ec5c41e645d71", + "Version": "1.6.0", + "Source": "Bioconductor", + "Hash": "2c824ac20bbb34bba92a9b5b0d600fec", "Requirements": [] }, - "tzdb": { - "Package": "tzdb", - "Version": "0.3.0", - "Source": "Repository", - "Repository": "CRAN", - "Hash": "b2e1cbce7c903eaf23ec05c58e59fb5e", - "Requirements": [ - "cpp11" - ] - }, "usethis": { "Package": "usethis", "Version": "1.6.1", @@ -1710,29 +1552,6 @@ "rlang" ] }, - "vroom": { - "Package": "vroom", - "Version": "1.6.1", - "Source": "Repository", - "Repository": "CRAN", - "Hash": "7015a74373b83ffaef64023f4a0f5033", - "Requirements": [ - "bit64", - "cli", - "cpp11", - "crayon", - "glue", - "hms", - "lifecycle", - "progress", - "rlang", - "tibble", - "tidyselect", - "tzdb", - "vctrs", - "withr" - ] - }, "whisker": { "Package": "whisker", "Version": "0.4", @@ -1751,10 +1570,10 @@ }, "xfun": { "Package": "xfun", - "Version": "0.16", + "Version": "0.36", "Source": "Repository", "Repository": "CRAN", - "Hash": "b4106139b90981a8bfea9c10bab0baf1", + "Hash": "f5baec54606751aa53ac9c0e05848ed6", "Requirements": [] }, "xml2": { @@ -1793,15 +1612,9 @@ }, "zlibbioc": { "Package": "zlibbioc", - "Version": "1.34.0", - "Source": "URL", - "RemoteType": "url", - "RemoteUrl": "https://bioconductor.org/packages/3.11/bioc/src/contrib/zlibbioc_1.34.0.tar.gz", - "git_url": "https://git.bioconductor.org/packages/zlibbioc", - "git_branch": "RELEASE_3_11", - "git_last_commit": "2db035c", - "git_last_commit_date": "2020-04-27", - "Hash": "d9bbfbd67e8c4bced2f5c1ce4251a744", + "Version": "1.24.0", + "Source": "Bioconductor", + "Hash": "75640bcdaa390770a672332c904642db", "Requirements": [] } } diff --git a/common/dockerfiles/Dockerfile.migrations b/common/dockerfiles/Dockerfile.migrations index 8688f12f1..00e704fa2 100644 --- a/common/dockerfiles/Dockerfile.migrations +++ b/common/dockerfiles/Dockerfile.migrations @@ -1,26 +1,19 @@ -FROM python:3.8.5-slim-buster +FROM ccdlstaging/dr_base:latest -RUN groupadd user && useradd --create-home --home-dir /home/user -g user user -WORKDIR /home/user - -RUN apt-get update -qq && \ - apt-get install -y \ - gcc \ - libpq-dev +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Needed for migration 0006 -COPY config/ config +WORKDIR /home/user COPY common/requirements.txt . - -RUN pip install -r requirements.txt +RUN pip install --ignore-installed --no-cache-dir -r requirements.txt COPY common/ . +COPY config config ARG SYSTEM_VERSION - -ENV SYSTEM_VERSION $SYSTEM_VERSION +ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user -ENTRYPOINT [""] +ENTRYPOINT [] diff --git a/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py b/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py index 00d8a637d..21ef3fe11 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py @@ -51,7 +51,8 @@ def handle(self, *args, **options): while True: for experiment in page.object_list: logger.debug( - "Refreshing metadata for an experiment.", experiment=experiment.accession_code + "Refreshing metadata for an experiment.", + experiment=experiment.accession_code, ) try: if experiment.source_database == "SRA": @@ -70,12 +71,10 @@ def handle(self, *args, **options): GeoSurveyor._apply_metadata_to_experiment(experiment, gse) elif experiment.source_database == "ARRAY_EXPRESS": - request_url = EXPERIMENTS_URL + experiment.accession_code - experiment_request = utils.requests_retry_session().get( - request_url, timeout=60 - ) try: - parsed_json = experiment_request.json()["experiments"]["experiment"][0] + metadata = ArrayExpressSurveyor._get_experiment_data( + experiment.accession_code + ) except KeyError: logger.error( "Remote experiment has no Experiment data!", @@ -83,7 +82,7 @@ def handle(self, *args, **options): survey_job=self.survey_job.id, ) continue - ArrayExpressSurveyor._apply_metadata_to_experiment(experiment, parsed_json) + ArrayExpressSurveyor._apply_metadata_to_experiment(experiment, metadata) experiment.save() diff --git a/foreman/data_refinery_foreman/surveyor/array_express.py b/foreman/data_refinery_foreman/surveyor/array_express.py index f99568454..e99cb5863 100644 --- a/foreman/data_refinery_foreman/surveyor/array_express.py +++ b/foreman/data_refinery_foreman/surveyor/array_express.py @@ -1,6 +1,5 @@ -from typing import Dict, List - -from django.utils.dateparse import parse_date +from datetime import datetime +from typing import Dict, List, Tuple from data_refinery_common.enums import Downloaders from data_refinery_common.logging import get_and_configure_logger @@ -27,7 +26,9 @@ logger = get_and_configure_logger(__name__) -EXPERIMENTS_URL = "https://www.ebi.ac.uk/arrayexpress/json/v3/experiments/" +EXPERIMENTS_URL = "https://www.ebi.ac.uk/biostudies/api/v1/studies/" +IDF_URL_TEMPLATE = "https://www.ebi.ac.uk/arrayexpress/files/{code}/{code}.idf.txt" +SDRF_URL_TEMPLATE = "https://www.ebi.ac.uk/biostudies/files/{code}/{code}.sdrf.txt" SAMPLES_URL = EXPERIMENTS_URL + "{}/samples" UNKNOWN = "UNKNOWN" @@ -40,37 +41,81 @@ class ArrayExpressSurveyor(ExternalSourceSurveyor): def source_type(self): return Downloaders.ARRAY_EXPRESS.value - @staticmethod - def _get_last_update_date(parsed_json: Dict) -> str: - if "lastupdatedate" in parsed_json: - return parsed_json["lastupdatedate"] - else: - return parsed_json["releasedate"] + def _parse_api_info_response(data: Dict) -> Dict: + return { + "file_count": data["files"], + "ftp_link": data["ftpLink"], + "last_updated_at": datetime.fromtimestamp(data["modified"] / 1000), + "released_at": datetime.fromtimestamp(data["released"] / 1000), + } @classmethod - def _apply_metadata_to_experiment(cls, experiment_object: Experiment, parsed_json: Dict): - # We aren't sure these fields will be populated, or how many there will be. - # Try to join them all together, or set a sensible default. - experiment_descripton = "" - if "description" in parsed_json and len(parsed_json["description"]) > 0: - for description_item in parsed_json["description"]: - if "text" in description_item: - experiment_descripton = experiment_descripton + description_item["text"] + "\n" - - if experiment_descripton == "": - experiment_descripton = "Description not available.\n" - - experiment_object.source_database = "ARRAY_EXPRESS" - experiment_object.title = parsed_json["name"] - # This will need to be updated if we ever use Array - # Express to get other kinds of data. - experiment_object.technology = "MICROARRAY" - experiment_object.description = experiment_descripton - - experiment_object.source_first_published = parse_date(parsed_json["releasedate"]) - experiment_object.source_last_modified = parse_date(cls._get_last_update_date(parsed_json)) - - def create_experiment_from_api(self, experiment_accession_code: str) -> (Experiment, Dict): + def _parse_api_data_response(cls, data: Dict) -> Dict: + parsed_data = {} + for attribute in data["section"]["attributes"]: + attribute_name = attribute["name"] + if attribute_name not in {"Description", "Organism", "Title"}: + continue + parsed_data[attribute_name.lower()] = attribute["value"] + + for subsection in data["section"]["subsections"]: + for subsection_entry in subsection: + # Protocols. + if isinstance(subsection_entry, dict) and subsection_entry["type"] == "Protocols": + if "protocols" not in parsed_data: + parsed_data["protocols"] = [] + + protocol = {} + for attribute in subsection_entry["attributes"]: + if "value" not in attribute: + continue + protocol[attribute["name"].lower()] = attribute["value"] + if protocol: + parsed_data["protocols"].append(protocol) + + # Array Designs. + elif subsection["type"] == "Assays and Data": + array_designs = [] + for assays_data_subsection in subsection["subsections"]: + if assays_data_subsection["type"] == "Array Designs": + for link in assays_data_subsection["links"]: + for link_entry in link: + array_designs.append(link_entry["url"]) + parsed_data["array_designs"] = array_designs + + return parsed_data + + @classmethod + def _get_experiment_data(cls, accession_code: str) -> Dict: + experiment_url = f"{EXPERIMENTS_URL}{accession_code}" + data = {"url": experiment_url} + + # Get experiment main data. + response = utils.requests_retry_session().get(experiment_url, timeout=60).json() + data.update(cls._parse_api_data_response(response)) + + # Get experiment info. + response = ( + utils.requests_retry_session() + .get(f"{EXPERIMENTS_URL}{accession_code}/info", timeout=60) + .json() + ) + info = cls._parse_api_info_response(response) + + data.update(info) + return data + + @classmethod + def _apply_metadata_to_experiment(cls, experiment: Experiment, metadata: Dict): + experiment.title = metadata["title"] + experiment.description = metadata.get("description", "Description not available.") + experiment.source_database = "ARRAY_EXPRESS" + # This will need to be updated if we ever use Array Express to get other kinds of data. + experiment.technology = "MICROARRAY" + experiment.source_first_published = metadata["released_at"] + experiment.source_last_modified = metadata["last_updated_at"] + + def create_experiment_from_api(self, experiment_accession_code: str) -> Tuple[Experiment, Dict]: """Given an experiment accession code, create an Experiment object. Also returns a dictionary of additional information about the @@ -78,184 +123,154 @@ def create_experiment_from_api(self, experiment_accession_code: str) -> (Experim Will raise an UnsupportedPlatformException if this experiment was conducted using a platform which we don't support. - - See an example at: https://www.ebi.ac.uk/arrayexpress/json/v3/experiments/E-MTAB-3050/sample """ - request_url = EXPERIMENTS_URL + experiment_accession_code - experiment_request = utils.requests_retry_session().get(request_url, timeout=60) try: - parsed_json = experiment_request.json()["experiments"]["experiment"][0] + experiment_data = self._get_experiment_data(accession_code=experiment_accession_code) except KeyError: logger.error( - "Remote experiment has no Experiment data!", + "Could not collect data from remote experiment source!", experiment_accession_code=experiment_accession_code, survey_job=self.survey_job.id, ) raise - experiment = {} - experiment["name"] = parsed_json["name"] - experiment["experiment_accession_code"] = experiment_accession_code - + array_designs = experiment_data["array_designs"] # This experiment has no platform at all, and is therefore useless. - if "arraydesign" not in parsed_json or len(parsed_json["arraydesign"]) == 0: + if not array_designs: logger.warn( - "Remote experiment has no arraydesign listed.", + "Remote experiment has no array design listed.", experiment_accession_code=experiment_accession_code, survey_job=self.survey_job.id, ) raise UnsupportedPlatformException - # If there is more than one arraydesign listed in the experiment + + platforms = {} + # If there is more than one array design listed in the experiment # then there is no other way to determine which array was used # for which sample other than looking at the header of the CEL # file. That obviously cannot happen until the CEL file has been # downloaded so we can just mark it as UNKNOWN and let the # downloader inspect the downloaded file to determine the # array then. - elif ( - len(parsed_json["arraydesign"]) != 1 or "accession" not in parsed_json["arraydesign"][0] - ): - experiment["platform_accession_code"] = UNKNOWN - experiment["platform_accession_name"] = UNKNOWN - experiment["manufacturer"] = UNKNOWN + if len(array_designs) != 1: + platforms["platform_accession_code"] = UNKNOWN + platforms["platform_accession_name"] = UNKNOWN + platforms["manufacturer"] = UNKNOWN else: - external_accession = parsed_json["arraydesign"][0]["accession"] + external_accession = array_designs[0] for platform in get_supported_microarray_platforms(): if platform["external_accession"] == external_accession: - experiment["platform_accession_code"] = get_normalized_platform( + platforms["platform_accession_code"] = get_normalized_platform( platform["platform_accession"] ) - # Illumina appears in the accession codes for # platforms manufactured by Illumina - if "ILLUMINA" in experiment["platform_accession_code"].upper(): - experiment["manufacturer"] = "ILLUMINA" - experiment["platform_accession_name"] = platform["platform_accession"] + if "ILLUMINA" in platforms["platform_accession_code"].upper(): + platforms["manufacturer"] = "ILLUMINA" + platforms["platform_accession_name"] = platform["platform_accession"] + # It's not Illumina, the only other supported Microarray platform is + # Affy. As our list of supported platforms grows this logic will + # need to get more sophisticated. else: - # It's not Illumina, the only other supported Microarray platform is - # Affy. As our list of supported platforms grows this logic will - # need to get more sophisticated. - experiment["manufacturer"] = "AFFYMETRIX" + platforms["manufacturer"] = "AFFYMETRIX" platform_mapping = get_readable_affymetrix_names() - experiment["platform_accession_name"] = platform_mapping[ + platforms["platform_accession_name"] = platform_mapping[ platform["platform_accession"] ] - if "platform_accession_code" not in experiment: + if "platform_accession_code" not in platforms: # We don't know what platform this accession corresponds to. - experiment["platform_accession_code"] = external_accession - experiment["platform_accession_name"] = UNKNOWN - experiment["manufacturer"] = UNKNOWN - - experiment["release_date"] = parsed_json["releasedate"] - experiment["last_update_date"] = self._get_last_update_date(parsed_json) + platforms["platform_accession_code"] = external_accession + platforms["platform_accession_name"] = UNKNOWN + platforms["manufacturer"] = UNKNOWN - # Create the experiment object + # Create the experiment. try: - experiment_object = Experiment.objects.get(accession_code=experiment_accession_code) + experiment = Experiment.objects.get(accession_code=experiment_accession_code) logger.debug( "Experiment already exists, skipping object creation.", experiment_accession_code=experiment_accession_code, survey_job=self.survey_job.id, ) except Experiment.DoesNotExist: - experiment_object = Experiment() - experiment_object.accession_code = experiment_accession_code - experiment_object.source_url = request_url - ArrayExpressSurveyor._apply_metadata_to_experiment(experiment_object, parsed_json) - experiment_object.save() + experiment = Experiment() + experiment.accession_code = experiment_accession_code + experiment.source_url = experiment_data["url"] + self._apply_metadata_to_experiment(experiment, experiment_data) + experiment.save() json_xa = ExperimentAnnotation() - json_xa.experiment = experiment_object - json_xa.data = parsed_json + json_xa.experiment = experiment + json_xa.data = experiment_data json_xa.is_ccdl = False json_xa.save() - # Fetch and parse the IDF/SDRF file for any other fields - IDF_URL_TEMPLATE = "https://www.ebi.ac.uk/arrayexpress/files/{code}/{code}.idf.txt" + # Fetch and parse the IDF/SDRF file for any other fields. idf_url = IDF_URL_TEMPLATE.format(code=experiment_accession_code) idf_text = utils.requests_retry_session().get(idf_url, timeout=60).text - lines = idf_text.split("\n") - idf_dict = {} - for line in lines: - keyval = line.strip().split("\t") - if len(keyval) == 2: - idf_dict[keyval[0]] = keyval[1] - elif len(keyval) > 2: - idf_dict[keyval[0]] = keyval[1:] + idf_data = {} + for line in idf_text.split("\n"): + key_val = line.strip().split("\t") + if len(key_val) == 2: + idf_data[key_val[0]] = key_val[1] + elif len(key_val) > 2: + idf_data[key_val[0]] = key_val[1:] idf_xa = ExperimentAnnotation() - idf_xa.data = idf_dict - idf_xa.experiment = experiment_object + idf_xa.data = idf_data + idf_xa.experiment = experiment idf_xa.is_ccdl = False idf_xa.save() - if "Investigation Title" in idf_dict and isinstance( - idf_dict["Investigation Title"], str + if "Investigation Title" in idf_data and isinstance( + idf_data["Investigation Title"], str ): - experiment_object.title = idf_dict["Investigation Title"] - if "Person Affiliation" in idf_dict: - # This is very rare, ex: E-MEXP-32 - if isinstance(idf_dict["Person Affiliation"], list): + experiment.title = idf_data["Investigation Title"] - unique_people = list(set(idf_dict["Person Affiliation"])) - experiment_object.submitter_institution = ", ".join(unique_people)[:255] + if "Person Affiliation" in idf_data: + # This is very rare, ex: E-MEXP-32 + if isinstance(idf_data["Person Affiliation"], list): + unique_people = list(set(idf_data["Person Affiliation"])) + experiment.submitter_institution = ", ".join(unique_people)[:255] else: - experiment_object.submitter_institution = idf_dict["Person Affiliation"] - - # Get protocol_description from "/protocols" - # instead of from idf_dict, because the former provides more - # details. - protocol_url = request_url + "/protocols" - protocol_request = utils.requests_retry_session().get(protocol_url, timeout=60) - try: - experiment_object.protocol_description = protocol_request.json()["protocols"] - except KeyError: - logger.warning( - "Remote experiment has no protocol data!", - experiment_accession_code=experiment_accession_code, - survey_job=self.survey_job.id, - ) + experiment.submitter_institution = idf_data["Person Affiliation"] - if "Publication Title" in idf_dict: + if "Publication Title" in idf_data: # This will happen for some superseries. # Ex: E-GEOD-29536 # Assume most recent is "best:, store the rest in experiment annotation. - if isinstance(idf_dict["Publication Title"], list): - experiment_object.publication_title = "; ".join(idf_dict["Publication Title"]) + if isinstance(idf_data["Publication Title"], list): + experiment.publication_title = "; ".join(idf_data["Publication Title"]) else: - experiment_object.publication_title = idf_dict["Publication Title"] - experiment_object.has_publication = True - if "Publication DOI" in idf_dict: - if isinstance(idf_dict["Publication DOI"], list): - experiment_object.publication_doi = ", ".join(idf_dict["Publication DOI"]) + experiment.publication_title = idf_data["Publication Title"] + experiment.has_publication = True + + if "Publication DOI" in idf_data: + experiment.has_publication = True + if isinstance(idf_data["Publication DOI"], list): + experiment.publication_doi = ", ".join(idf_data["Publication DOI"]) else: - experiment_object.publication_doi = idf_dict["Publication DOI"] - experiment_object.has_publication = True - if "PubMed ID" in idf_dict: - if isinstance(idf_dict["PubMed ID"], list): - experiment_object.pubmed_id = ", ".join(idf_dict["PubMed ID"]) + experiment.publication_doi = idf_data["Publication DOI"] + + if "PubMed ID" in idf_data: + experiment.has_publication = True + if isinstance(idf_data["PubMed ID"], list): + experiment.pubmed_id = ", ".join(idf_data["PubMed ID"]) else: - experiment_object.pubmed_id = idf_dict["PubMed ID"] - experiment_object.has_publication = True + experiment.pubmed_id = idf_data["PubMed ID"] # Scrape publication title and authorship from Pubmed - if experiment_object.pubmed_id: - pubmed_metadata = utils.get_title_and_authors_for_pubmed_id( - experiment_object.pubmed_id - ) - experiment_object.publication_title = pubmed_metadata[0] - experiment_object.publication_authors = pubmed_metadata[1] - - experiment_object.save() + if experiment.pubmed_id: + pubmed_metadata = utils.get_title_and_authors_for_pubmed_id(experiment.pubmed_id) + experiment.publication_title = pubmed_metadata[0] + experiment.publication_authors = pubmed_metadata[1] - platform_dict = {} - for k in ("platform_accession_code", "platform_accession_name", "manufacturer"): - platform_dict[k] = experiment[k] + experiment.protocol_description = experiment_data["protocols"] + experiment.save() - return experiment_object, platform_dict + return experiment, platforms def determine_sample_accession( self, diff --git a/foreman/run_tests.sh b/foreman/run_tests.sh index a78a128ab..e35df351b 100755 --- a/foreman/run_tests.sh +++ b/foreman/run_tests.sh @@ -42,5 +42,6 @@ fi docker run -t $INTERACTIVE \ --add-host=database:"$DB_HOST_IP" \ --env-file foreman/environments/test \ + --platform linux/amd64 \ --volume "$volume_directory":/home/user/data_store \ ccdlstaging/dr_foreman bash -c "$(run_tests_with_coverage --exclude-tag=manual "$@")" diff --git a/foreman/tests/__init__.py b/foreman/tests/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/tests/foreman/__init__.py b/foreman/tests/foreman/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/tests/foreman/management/__init__.py b/foreman/tests/foreman/management/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/tests/foreman/management/commands/__init__.py b/foreman/tests/foreman/management/commands/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_correct_affy_cdfs.py b/foreman/tests/foreman/management/commands/test_correct_affy_cdfs.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/management/commands/test_correct_affy_cdfs.py rename to foreman/tests/foreman/management/commands/test_correct_affy_cdfs.py diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_create_compendia.py b/foreman/tests/foreman/management/commands/test_create_compendia.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/management/commands/test_create_compendia.py rename to foreman/tests/foreman/management/commands/test_create_compendia.py diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_create_missing_downloader_jobs.py b/foreman/tests/foreman/management/commands/test_create_missing_downloader_jobs.py similarity index 96% rename from foreman/data_refinery_foreman/foreman/management/commands/test_create_missing_downloader_jobs.py rename to foreman/tests/foreman/management/commands/test_create_missing_downloader_jobs.py index e8b545c57..fa85d17db 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/test_create_missing_downloader_jobs.py +++ b/foreman/tests/foreman/management/commands/test_create_missing_downloader_jobs.py @@ -3,15 +3,9 @@ from data_refinery_common.models import ( DownloaderJob, DownloaderJobOriginalFileAssociation, - Experiment, - ExperimentSampleAssociation, - Organism, OriginalFile, OriginalFileSampleAssociation, - ProcessorJob, Sample, - SurveyJob, - SurveyJobKeyValue, ) from data_refinery_foreman.foreman.management.commands.create_missing_downloader_jobs import Command diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_create_missing_processor_jobs.py b/foreman/tests/foreman/management/commands/test_create_missing_processor_jobs.py similarity index 99% rename from foreman/data_refinery_foreman/foreman/management/commands/test_create_missing_processor_jobs.py rename to foreman/tests/foreman/management/commands/test_create_missing_processor_jobs.py index a30dd191f..a45d68312 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/test_create_missing_processor_jobs.py +++ b/foreman/tests/foreman/management/commands/test_create_missing_processor_jobs.py @@ -3,16 +3,11 @@ from data_refinery_common.models import ( DownloaderJob, DownloaderJobOriginalFileAssociation, - Experiment, - ExperimentSampleAssociation, - Organism, OriginalFile, OriginalFileSampleAssociation, ProcessorJob, ProcessorJobOriginalFileAssociation, Sample, - SurveyJob, - SurveyJobKeyValue, ) from data_refinery_foreman.foreman.management.commands.create_missing_processor_jobs import Command diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_create_quantpendia.py b/foreman/tests/foreman/management/commands/test_create_quantpendia.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/management/commands/test_create_quantpendia.py rename to foreman/tests/foreman/management/commands/test_create_quantpendia.py diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_import_external_sample_attributes.py b/foreman/tests/foreman/management/commands/test_import_external_sample_attributes.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/management/commands/test_import_external_sample_attributes.py rename to foreman/tests/foreman/management/commands/test_import_external_sample_attributes.py diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_import_external_sample_keywords.py b/foreman/tests/foreman/management/commands/test_import_external_sample_keywords.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/management/commands/test_import_external_sample_keywords.py rename to foreman/tests/foreman/management/commands/test_import_external_sample_keywords.py diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_organism_shepherd.py b/foreman/tests/foreman/management/commands/test_organism_shepherd.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/management/commands/test_organism_shepherd.py rename to foreman/tests/foreman/management/commands/test_organism_shepherd.py diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_rerun_salmon_old_samples.py b/foreman/tests/foreman/management/commands/test_rerun_salmon_old_samples.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/management/commands/test_rerun_salmon_old_samples.py rename to foreman/tests/foreman/management/commands/test_rerun_salmon_old_samples.py diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_retry_samples.py b/foreman/tests/foreman/management/commands/test_retry_samples.py similarity index 92% rename from foreman/data_refinery_foreman/foreman/management/commands/test_retry_samples.py rename to foreman/tests/foreman/management/commands/test_retry_samples.py index ef5023a34..26ab04f52 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/test_retry_samples.py +++ b/foreman/tests/foreman/management/commands/test_retry_samples.py @@ -4,24 +4,15 @@ from django.utils import timezone from data_refinery_common.models import ( - ComputationalResult, - ComputationalResultAnnotation, - ComputedFile, DownloaderJob, - DownloaderJobOriginalFileAssociation, Experiment, - ExperimentOrganismAssociation, - ExperimentResultAssociation, ExperimentSampleAssociation, Organism, - OrganismIndex, OriginalFile, OriginalFileSampleAssociation, - Processor, ProcessorJob, ProcessorJobOriginalFileAssociation, Sample, - SampleResultAssociation, ) from data_refinery_foreman.foreman.management.commands.retry_samples import retry_by_regex diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_run_tximport.py b/foreman/tests/foreman/management/commands/test_run_tximport.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/management/commands/test_run_tximport.py rename to foreman/tests/foreman/management/commands/test_run_tximport.py diff --git a/foreman/data_refinery_foreman/foreman/management/commands/test_update_experiment_metadata.py b/foreman/tests/foreman/management/commands/test_update_experiment_metadata.py similarity index 74% rename from foreman/data_refinery_foreman/foreman/management/commands/test_update_experiment_metadata.py rename to foreman/tests/foreman/management/commands/test_update_experiment_metadata.py index b87517356..2b2239f95 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/test_update_experiment_metadata.py +++ b/foreman/tests/foreman/management/commands/test_update_experiment_metadata.py @@ -1,5 +1,3 @@ -from unittest.mock import patch - from django.test import TransactionTestCase from data_refinery_common.models import Experiment, ExperimentSampleAssociation, Sample @@ -7,24 +5,26 @@ class SurveyTestCase(TransactionTestCase): + BAD_TITLE = ( + "BAD TITLE: Accession is currently private " + "and is scheduled to be released on Jan 01, 1970." + ) + def tearDown(self): Experiment.objects.all().delete() def test_sra_experiment_missing_metadata(self): """Tests that an SRA experiment has its missing metadata added.""" - # 1. Create an experiment with a bad title - BAD_TITLE = "GEO accession GSE1337 is currently private\ - and is scheduled to be released on Jan 01, 1970." - + # 1. Create an experiment with a bad title. experiment = Experiment() experiment.accession_code = "DRP003977" experiment.source_database = "SRA" - experiment.title = BAD_TITLE + experiment.title = SurveyTestCase.BAD_TITLE experiment.save() # 2. We need to add a sample because the way that the SRA surveyor finds metadata is - # through run accessions + # through run accessions. sample = Sample() sample.accession_code = "DRR002116" sample.technology = "RNA-SEQ" @@ -38,20 +38,21 @@ def test_sra_experiment_missing_metadata(self): command = Command() command.handle() - # Test that the title was fixed + # Test that the title was fixed. + experiment = Experiment.objects.get_or_create(accession_code=experiment.accession_code)[0] self.assertNotEqual( - Experiment.objects.get_or_create(accession_code=experiment.accession_code)[0].title, - BAD_TITLE, + experiment.title, + SurveyTestCase.BAD_TITLE, ) - # Run the command again to make sure that it does not fail if there are no changes + # Run the command again to make sure that it does not fail if there are no changes. command = Command() command.handle() def test_sra_experiment_missing_alternate_accession(self): """Tests that an SRA experiment has its missing alternate_accession_code added.""" - # 1. Create an experiment without an alternate_accession_code + # 1. Create an experiment without an alternate_accession_code. experiment = Experiment() experiment.accession_code = "SRP094947" experiment.source_database = "SRA" @@ -59,7 +60,7 @@ def test_sra_experiment_missing_alternate_accession(self): experiment.save() # 2. We need to add a sample because the way that the SRA surveyor finds metadata is - # through run accessions + # through run accessions. sample = Sample() sample.accession_code = "SRR5099111" sample.technology = "RNA-SEQ" @@ -82,27 +83,25 @@ def test_sra_experiment_missing_alternate_accession(self): def test_geo_experiment_missing_metadata(self): """Tests that a GEO experiment has its missing metadata added.""" - # 1. Create an experiment with a bad title - BAD_TITLE = "GEO accession GSE1337 is currently private\ - and is scheduled to be released on Jan 01, 1970." - + # 1. Create an experiment with a bad title. experiment = Experiment() experiment.accession_code = "GSE11915" experiment.source_database = "GEO" - experiment.title = BAD_TITLE + experiment.title = SurveyTestCase.BAD_TITLE experiment.save() # 2. Setup is done, actually run the command. command = Command() command.handle() - # Test that the title was fixed + # Test that the title was fixed. + experiment = Experiment.objects.get_or_create(accession_code=experiment.accession_code)[0] self.assertNotEqual( - Experiment.objects.get_or_create(accession_code=experiment.accession_code)[0].title, - BAD_TITLE, + experiment.title, + SurveyTestCase.BAD_TITLE, ) - # Run the command again to make sure that it does not fail if there are no changes + # Run the command again to make sure that it does not fail if there are no changes. command = Command() command.handle() @@ -110,25 +109,23 @@ def test_array_express_experiment_missing_metadata(self): """Tests that an ArrayExpress experiment has its missing metadata added.""" # 1. Create an experiment with a bad title - BAD_TITLE = "GEO accession GSE1337 is currently private\ - and is scheduled to be released on Jan 01, 1970." - experiment = Experiment() experiment.accession_code = "E-MTAB-3050" experiment.source_database = "ARRAY_EXPRESS" - experiment.title = BAD_TITLE + experiment.title = SurveyTestCase.BAD_TITLE experiment.save() # 2. Setup is done, actually run the command. command = Command() command.handle() - # Test that the title was fixed + # Test that the title was fixed. + experiment = Experiment.objects.get_or_create(accession_code=experiment.accession_code)[0] self.assertNotEqual( - Experiment.objects.get_or_create(accession_code=experiment.accession_code)[0].title, - BAD_TITLE, + experiment.title, + SurveyTestCase.BAD_TITLE, ) - # Run the command again to make sure that it does not fail if there are no changes + # Run the command again to make sure that it does not fail if there are no changes. command = Command() command.handle() diff --git a/foreman/data_refinery_foreman/foreman/test_downloader_job_manager.py b/foreman/tests/foreman/test_downloader_job_manager.py similarity index 99% rename from foreman/data_refinery_foreman/foreman/test_downloader_job_manager.py rename to foreman/tests/foreman/test_downloader_job_manager.py index 1de377a0f..9b3f6b15d 100644 --- a/foreman/data_refinery_foreman/foreman/test_downloader_job_manager.py +++ b/foreman/tests/foreman/test_downloader_job_manager.py @@ -5,9 +5,10 @@ from django.test import TestCase from django.utils import timezone +from tests.foreman.utils import create_downloader_job + from data_refinery_common.models import DownloaderJob from data_refinery_foreman.foreman import downloader_job_manager, utils -from data_refinery_foreman.foreman.test_utils import create_downloader_job # For use in tests that test the JOB_CREATED_AT_CUTOFF functionality. DAY_BEFORE_JOB_CUTOFF = utils.JOB_CREATED_AT_CUTOFF - datetime.timedelta(days=1) diff --git a/foreman/data_refinery_foreman/foreman/test_end_to_end.py b/foreman/tests/foreman/test_end_to_end.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/test_end_to_end.py rename to foreman/tests/foreman/test_end_to_end.py diff --git a/foreman/data_refinery_foreman/foreman/test_job_control.py b/foreman/tests/foreman/test_job_control.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/test_job_control.py rename to foreman/tests/foreman/test_job_control.py diff --git a/foreman/data_refinery_foreman/foreman/test_job_requeuing.py b/foreman/tests/foreman/test_job_requeuing.py similarity index 97% rename from foreman/data_refinery_foreman/foreman/test_job_requeuing.py rename to foreman/tests/foreman/test_job_requeuing.py index c6fc7f622..d87f4bcf5 100644 --- a/foreman/data_refinery_foreman/foreman/test_job_requeuing.py +++ b/foreman/tests/foreman/test_job_requeuing.py @@ -5,13 +5,10 @@ from django.test import TestCase from django.utils import timezone +from tests.foreman.utils import create_downloader_job, create_processor_job, create_survey_job + from data_refinery_common.models import DownloaderJob, ProcessorJob, SurveyJob from data_refinery_foreman.foreman import job_requeuing -from data_refinery_foreman.foreman.test_utils import ( - create_downloader_job, - create_processor_job, - create_survey_job, -) def fake_send_job(job_type, job, is_dispatch=False): diff --git a/foreman/data_refinery_foreman/foreman/test_processor_job_manager.py b/foreman/tests/foreman/test_processor_job_manager.py similarity index 99% rename from foreman/data_refinery_foreman/foreman/test_processor_job_manager.py rename to foreman/tests/foreman/test_processor_job_manager.py index 5754a65b7..3c2e461af 100644 --- a/foreman/data_refinery_foreman/foreman/test_processor_job_manager.py +++ b/foreman/tests/foreman/test_processor_job_manager.py @@ -5,9 +5,10 @@ from django.test import TestCase from django.utils import timezone +from tests.foreman.utils import create_processor_job + from data_refinery_common.models import ProcessorJob from data_refinery_foreman.foreman import processor_job_manager, utils -from data_refinery_foreman.foreman.test_utils import create_processor_job # For use in tests that test the JOB_CREATED_AT_CUTOFF functionality. DAY_BEFORE_JOB_CUTOFF = utils.JOB_CREATED_AT_CUTOFF - datetime.timedelta(days=1) diff --git a/foreman/data_refinery_foreman/foreman/test_survey_job_manager.py b/foreman/tests/foreman/test_survey_job_manager.py similarity index 99% rename from foreman/data_refinery_foreman/foreman/test_survey_job_manager.py rename to foreman/tests/foreman/test_survey_job_manager.py index 74e71f868..7c2b6bbfd 100644 --- a/foreman/data_refinery_foreman/foreman/test_survey_job_manager.py +++ b/foreman/tests/foreman/test_survey_job_manager.py @@ -6,9 +6,10 @@ from django.test import TestCase from django.utils import timezone +from tests.foreman.utils import create_survey_job + from data_refinery_common.models import SurveyJob from data_refinery_foreman.foreman import survey_job_manager, utils -from data_refinery_foreman.foreman.test_utils import create_survey_job # For use in tests that test the JOB_CREATED_AT_CUTOFF functionality. DAY_BEFORE_JOB_CUTOFF = utils.JOB_CREATED_AT_CUTOFF - datetime.timedelta(days=1) diff --git a/foreman/data_refinery_foreman/foreman/test_utils.py b/foreman/tests/foreman/utils.py similarity index 100% rename from foreman/data_refinery_foreman/foreman/test_utils.py rename to foreman/tests/foreman/utils.py diff --git a/foreman/tests/settings.py b/foreman/tests/settings.py new file mode 100644 index 000000000..ad7d4a4a9 --- /dev/null +++ b/foreman/tests/settings.py @@ -0,0 +1,5 @@ +""" +Django settings for data_refinery_foreman project tests. +""" + +from data_refinery_foreman.settings import * diff --git a/foreman/tests/surveyor/management/__init__.py b/foreman/tests/surveyor/management/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/tests/surveyor/management/commands/__init__.py b/foreman/tests/surveyor/management/commands/__init__.py new file mode 100644 index 000000000..e69de29bb diff --git a/foreman/data_refinery_foreman/surveyor/management/commands/test_dispatch_qn_jobs.py b/foreman/tests/surveyor/management/commands/test_dispatch_qn_jobs.py similarity index 100% rename from foreman/data_refinery_foreman/surveyor/management/commands/test_dispatch_qn_jobs.py rename to foreman/tests/surveyor/management/commands/test_dispatch_qn_jobs.py diff --git a/foreman/data_refinery_foreman/surveyor/management/commands/test_unsurvey.py b/foreman/tests/surveyor/management/commands/test_unsurvey.py similarity index 100% rename from foreman/data_refinery_foreman/surveyor/management/commands/test_unsurvey.py rename to foreman/tests/surveyor/management/commands/test_unsurvey.py diff --git a/foreman/data_refinery_foreman/surveyor/test_array_express.py b/foreman/tests/surveyor/test_array_express.py similarity index 100% rename from foreman/data_refinery_foreman/surveyor/test_array_express.py rename to foreman/tests/surveyor/test_array_express.py diff --git a/foreman/data_refinery_foreman/surveyor/test_external_source.py b/foreman/tests/surveyor/test_external_source.py similarity index 100% rename from foreman/data_refinery_foreman/surveyor/test_external_source.py rename to foreman/tests/surveyor/test_external_source.py diff --git a/foreman/data_refinery_foreman/surveyor/test_geo.py b/foreman/tests/surveyor/test_geo.py similarity index 100% rename from foreman/data_refinery_foreman/surveyor/test_geo.py rename to foreman/tests/surveyor/test_geo.py diff --git a/foreman/data_refinery_foreman/surveyor/test_harmony.py b/foreman/tests/surveyor/test_harmony.py similarity index 100% rename from foreman/data_refinery_foreman/surveyor/test_harmony.py rename to foreman/tests/surveyor/test_harmony.py diff --git a/foreman/data_refinery_foreman/surveyor/test_sra.py b/foreman/tests/surveyor/test_sra.py similarity index 100% rename from foreman/data_refinery_foreman/surveyor/test_sra.py rename to foreman/tests/surveyor/test_sra.py diff --git a/foreman/data_refinery_foreman/surveyor/test_surveyor.py b/foreman/tests/surveyor/test_surveyor.py similarity index 100% rename from foreman/data_refinery_foreman/surveyor/test_surveyor.py rename to foreman/tests/surveyor/test_surveyor.py diff --git a/foreman/data_refinery_foreman/surveyor/test_transcriptome_index.py b/foreman/tests/surveyor/test_transcriptome_index.py similarity index 100% rename from foreman/data_refinery_foreman/surveyor/test_transcriptome_index.py rename to foreman/tests/surveyor/test_transcriptome_index.py diff --git a/foreman/data_refinery_foreman/test_imports.py b/foreman/tests/test_imports.py similarity index 53% rename from foreman/data_refinery_foreman/test_imports.py rename to foreman/tests/test_imports.py index ef1cf2633..9c2717e0e 100644 --- a/foreman/data_refinery_foreman/test_imports.py +++ b/foreman/tests/test_imports.py @@ -1,4 +1,4 @@ -from django.test import TestCase, tag +from django.test import TestCase class ImportTestCase(TestCase): @@ -28,33 +28,33 @@ def test_imports(self): import data_refinery_foreman.foreman.management.commands.retry_timed_out_jobs import data_refinery_foreman.foreman.management.commands.run_tximport import data_refinery_foreman.foreman.management.commands.stop_jobs - import data_refinery_foreman.foreman.management.commands.test_correct_affy_cdfs - import data_refinery_foreman.foreman.management.commands.test_create_compendia - import data_refinery_foreman.foreman.management.commands.test_create_missing_downloader_jobs - import data_refinery_foreman.foreman.management.commands.test_create_missing_processor_jobs - import data_refinery_foreman.foreman.management.commands.test_create_quantpendia - import data_refinery_foreman.foreman.management.commands.test_import_external_sample_attributes - import data_refinery_foreman.foreman.management.commands.test_import_external_sample_keywords - import data_refinery_foreman.foreman.management.commands.test_organism_shepherd - import data_refinery_foreman.foreman.management.commands.test_rerun_salmon_old_samples - import data_refinery_foreman.foreman.management.commands.test_retry_samples - import data_refinery_foreman.foreman.management.commands.test_run_tximport - import data_refinery_foreman.foreman.management.commands.test_update_experiment_metadata import data_refinery_foreman.foreman.management.commands.update_downloadable_samples import data_refinery_foreman.foreman.management.commands.update_experiment_metadata import data_refinery_foreman.foreman.management.commands.update_sample_metadata - import data_refinery_foreman.foreman.test_downloader_job_manager - import data_refinery_foreman.foreman.test_end_to_end - import data_refinery_foreman.foreman.test_job_control - import data_refinery_foreman.foreman.test_job_requeuing - import data_refinery_foreman.foreman.test_processor_job_manager - import data_refinery_foreman.foreman.test_survey_job_manager - import data_refinery_foreman.foreman.test_utils - import data_refinery_foreman.surveyor.management.commands.test_unsurvey - import data_refinery_foreman.surveyor.test_array_express - import data_refinery_foreman.surveyor.test_external_source - import data_refinery_foreman.surveyor.test_geo - import data_refinery_foreman.surveyor.test_harmony - import data_refinery_foreman.surveyor.test_sra - import data_refinery_foreman.surveyor.test_surveyor - import data_refinery_foreman.surveyor.test_transcriptome_index + import data_refinery_foreman.foreman.utils + import tests.foreman.management.commands.test_correct_affy_cdfs + import tests.foreman.management.commands.test_create_compendia + import tests.foreman.management.commands.test_create_missing_downloader_jobs + import tests.foreman.management.commands.test_create_missing_processor_jobs + import tests.foreman.management.commands.test_create_quantpendia + import tests.foreman.management.commands.test_import_external_sample_attributes + import tests.foreman.management.commands.test_import_external_sample_keywords + import tests.foreman.management.commands.test_organism_shepherd + import tests.foreman.management.commands.test_rerun_salmon_old_samples + import tests.foreman.management.commands.test_retry_samples + import tests.foreman.management.commands.test_run_tximport + import tests.foreman.management.commands.test_update_experiment_metadata + import tests.foreman.test_downloader_job_manager + import tests.foreman.test_end_to_end + import tests.foreman.test_job_control + import tests.foreman.test_job_requeuing + import tests.foreman.test_processor_job_manager + import tests.foreman.test_survey_job_manager + import tests.surveyor.management.commands.test_unsurvey + import tests.surveyor.test_array_express + import tests.surveyor.test_external_source + import tests.surveyor.test_geo + import tests.surveyor.test_harmony + import tests.surveyor.test_sra + import tests.surveyor.test_surveyor + import tests.surveyor.test_transcriptome_index diff --git a/foreman/data_refinery_foreman/test_runner.py b/foreman/tests/test_runner.py similarity index 100% rename from foreman/data_refinery_foreman/test_runner.py rename to foreman/tests/test_runner.py diff --git a/workers/R/dependencies/install_devtools_renv.R b/workers/R/dependencies/install_devtools_renv.R index 58c97546b..03b259f39 100644 --- a/workers/R/dependencies/install_devtools_renv.R +++ b/workers/R/dependencies/install_devtools_renv.R @@ -21,9 +21,9 @@ renv::install("backports@1.1.8") renv::install("rprojroot@1.3-2") renv::install("desc@1.2.0") renv::install("prettyunits@1.1.1") -renv::install("ps@1.3.3") +renv::install("ps@1.3.4") renv::install("processx@3.4.3") -renv::install("callr@3.4.3") +renv::install("callr@3.7.3") renv::install("pkgbuild@1.1.0") renv::install("rstudioapi@0.11") renv::install("pkgload@1.1.0") diff --git a/workers/R/renv_load.R b/workers/R/renv_load.R index 601e479b1..dd5caa080 100644 --- a/workers/R/renv_load.R +++ b/workers/R/renv_load.R @@ -10,7 +10,7 @@ options(renv.r.version = "3.4.4") options(renv.settings.use.cache = FALSE) install.packages("BiocInstaller") -install.packages("https://cloud.r-project.org/src/contrib/renv_0.16.0.tar.gz") +install.packages("https://cran.r-project.org/src/contrib/Archive/renv/renv_0.16.0.tar.gz") renv::consent(provided = TRUE) renv::restore(prompt = FALSE, rebuild = TRUE) diff --git a/workers/R/renv_save.R b/workers/R/renv_save.R index 7892c0a3a..a7196bae1 100644 --- a/workers/R/renv_save.R +++ b/workers/R/renv_save.R @@ -10,6 +10,6 @@ options(renv.r.version = "3.4.4") options(renv.settings.use.cache = FALSE) install.packages("BiocInstaller") -install.packages("https://cloud.r-project.org/src/contrib/renv_0.16.0.tar.gz") +install.packages("https://cran.r-project.org/src/contrib/Archive/renv/renv_0.16.0.tar.gz") renv::init(bioconductor = "3.6") diff --git a/workers/data_refinery_workers/processors/requirements.in b/workers/data_refinery_workers/processors/requirements.in index 1a02ecdbe..10394b9f0 100644 --- a/workers/data_refinery_workers/processors/requirements.in +++ b/workers/data_refinery_workers/processors/requirements.in @@ -1,6 +1,7 @@ boto3>=1.9.199 coveralls -django>=3.2.5 +django>=3.2,<4 +rpy2===3.4.5 matplotlib numpy pandas @@ -20,13 +21,14 @@ unicodecsv untangle>=1.1.1 # For visualization dask>=1.1.1 -xarray>=0.12.2 -datashader>=0.6.9 +xarray==0.16.2 +datashader==0.14.4 datashape>=0.5.2 holoviews>=1.11.2 selenium==3.141.0 -# For mocking out S3 calls for the smasher -vcrpy +# For mocking out S3 calls for the smasher. +vcrpy==4.1.1 django-computedfields>=0.1.5 pyyaml>=5.4 sqlparse>=0.4.2 +nose diff --git a/workers/data_refinery_workers/processors/requirements.txt b/workers/data_refinery_workers/processors/requirements.txt index 20dce991c..9c64659b3 100644 --- a/workers/data_refinery_workers/processors/requirements.txt +++ b/workers/data_refinery_workers/processors/requirements.txt @@ -1,91 +1,96 @@ # -# This file is autogenerated by pip-compile +# This file is autogenerated by pip-compile with python 3.9 # To update, run: # -# pip-compile requirements.in +# pip-compile --annotation-style=line workers/data_refinery_workers/processors/requirements.in # -asgiref==3.3.4 # via django -bokeh==2.3.2 # via dask, panel -boto3==1.17.92 # via -r requirements.in -botocore==1.20.92 # via boto3, s3transfer -certifi==2021.5.30 # via requests -chardet==4.0.0 # via requests -click==8.0.1 # via distributed -cloudpickle==1.6.0 # via dask, distributed -colorcet==2.0.6 # via datashader, holoviews -contextvars==2.4 # via distributed -coverage==5.5 # via coveralls -coveralls==3.1.0 # via -r requirements.in -cycler==0.10.0 # via matplotlib -dask[complete]==2021.3.0 # via -r requirements.in, datashader, distributed -datashader==0.13.0 # via -r requirements.in -datashape==0.5.2 # via -r requirements.in, datashader -distributed==2021.3.0 # via dask -django-computedfields==0.1.5 # via -r requirements.in -django==3.2.7 # via -r requirements.in +asgiref==3.6.0 # via django +bleach==6.0.0 # via panel +bokeh==2.4.3 # via panel +boto3==1.26.72 # via -r workers/data_refinery_workers/processors/requirements.in +botocore==1.29.72 # via boto3, s3transfer +certifi==2022.12.7 # via requests +cffi==1.15.1 # via rpy2 +charset-normalizer==3.0.1 # via requests +click==8.1.3 # via dask +cloudpickle==2.2.1 # via dask +colorcet==3.0.1 # via datashader, holoviews +contourpy==1.0.7 # via matplotlib +coverage==6.5.0 # via coveralls +coveralls==3.3.1 # via -r workers/data_refinery_workers/processors/requirements.in +cycler==0.11.0 # via matplotlib +dask==2023.2.0 # via -r workers/data_refinery_workers/processors/requirements.in, datashader +datashader==0.14.4 # via -r workers/data_refinery_workers/processors/requirements.in +datashape==0.5.2 # via -r workers/data_refinery_workers/processors/requirements.in, datashader +defusedxml==0.7.1 # via untangle +django==3.2.18 # via -r workers/data_refinery_workers/processors/requirements.in, django-computedfields, django-fast-update +django-computedfields==0.2.1 # via -r workers/data_refinery_workers/processors/requirements.in +django-fast-update==0.2.1 # via django-computedfields docopt==0.6.2 # via coveralls -fsspec==2021.6.0 # via dask -heapdict==1.0.1 # via zict -holoviews==1.14.4 # via -r requirements.in -idna==2.10 # via requests, yarl -immutables==0.15 # via contextvars -importlib-metadata==4.5.0 # via click, markdown -jinja2==3.0.1 # via -r requirements.in, bokeh -jmespath==0.10.0 # via boto3, botocore -joblib==1.0.1 # via scikit-learn -kiwisolver==1.3.1 # via matplotlib -llvmlite==0.36.0 # via numba -locket==0.2.1 # via partd -markdown==3.3.4 # via panel -markupsafe==2.0.1 # via jinja2 -matplotlib==3.3.4 # via -r requirements.in +fonttools==4.38.0 # via matplotlib +fsspec==2023.1.0 # via dask +holoviews==1.15.4 # via -r workers/data_refinery_workers/processors/requirements.in +idna==3.4 # via requests, yarl +importlib-metadata==6.0.0 # via markdown +importlib-resources==5.10.2 # via matplotlib +jinja2==3.1.2 # via -r workers/data_refinery_workers/processors/requirements.in, bokeh, rpy2 +jmespath==1.0.1 # via boto3, botocore +joblib==1.2.0 # via scikit-learn +kiwisolver==1.4.4 # via matplotlib +llvmlite==0.39.1 # via numba +locket==1.0.0 # via partd +markdown==3.4.1 # via panel +markupsafe==2.1.2 # via jinja2 +matplotlib==3.7.0 # via -r workers/data_refinery_workers/processors/requirements.in mpmath==1.2.1 # via sympy -msgpack==1.0.2 # via distributed -multidict==5.1.0 # via yarl +multidict==6.0.4 # via yarl multipledispatch==0.6.0 # via datashape -numba==0.53.1 # via datashader -numpy==1.19.5 # via -r requirements.in, bokeh, dask, datashader, datashape, holoviews, matplotlib, numba, pandas, scikit-learn, scipy, xarray -packaging==20.9 # via bokeh -pandas==1.1.5 # via -r requirements.in, dask, datashader, holoviews, xarray -panel==0.11.3 # via holoviews -param==1.10.1 # via colorcet, datashader, holoviews, panel, pyct, pyviz-comms -partd==1.2.0 # via dask -pillow==8.3.2 # via -r requirements.in, bokeh, datashader, matplotlib -psutil==5.8.0 # via -r requirements.in, distributed -psycopg2-binary==2.8.6 # via -r requirements.in -pyct==0.4.8 # via colorcet, datashader, panel -pyparsing==2.4.7 # via matplotlib, packaging -python-dateutil==2.8.1 # via bokeh, botocore, datashape, matplotlib, pandas -pytz==2021.1 # via django, pandas, tzlocal -pyviz-comms==2.0.2 # via holoviews, panel -pyyaml==5.4.1 # via -r requirements.in, bokeh, dask, distributed, vcrpy -requests==2.25.1 # via -r requirements.in, coveralls, panel -retrying==1.3.3 # via -r requirements.in -s3transfer==0.4.2 # via boto3 -scikit-learn==0.24.2 # via -r requirements.in -scipy==1.5.4 # via -r requirements.in, datashader, scikit-learn -selenium==3.141.0 # via -r requirements.in -simplejson==3.17.2 # via -r requirements.in -six==1.16.0 # via cycler, multipledispatch, python-dateutil, retrying, vcrpy -sortedcontainers==2.4.0 # via distributed -sqlparse==0.4.2 # via -r requirements.in, django -sympy==1.8 # via -r requirements.in -tblib==1.7.0 # via distributed -threadpoolctl==2.1.0 # via scikit-learn -toolz==0.11.1 # via dask, distributed, partd -tornado==6.1 # via bokeh, distributed -tqdm==4.61.0 # via panel -typing-extensions==3.10.0.0 # via asgiref, bokeh, importlib-metadata, yarl -tzlocal==2.1 # via -r requirements.in -unicodecsv==0.14.1 # via -r requirements.in -untangle==1.1.1 # via -r requirements.in -urllib3==1.26.5 # via botocore, requests, selenium -vcrpy==4.1.1 # via -r requirements.in -wrapt==1.12.1 # via vcrpy -xarray==0.16.2 # via -r requirements.in, datashader -yarl==1.6.3 # via vcrpy -zict==2.0.0 # via distributed -zipp==3.4.1 # via importlib-metadata +nose==1.3.7 # via -r workers/data_refinery_workers/processors/requirements.in +numba==0.56.4 # via datashader +numpy==1.23.5 # via -r workers/data_refinery_workers/processors/requirements.in, bokeh, contourpy, datashader, datashape, holoviews, matplotlib, numba, pandas, scikit-learn, scipy, xarray +packaging==23.0 # via bokeh, dask, holoviews, matplotlib +pandas==1.5.3 # via -r workers/data_refinery_workers/processors/requirements.in, datashader, holoviews, xarray +panel==0.14.3 # via holoviews +param==1.12.3 # via datashader, holoviews, panel, pyct, pyviz-comms +partd==1.3.0 # via dask +pillow==9.4.0 # via -r workers/data_refinery_workers/processors/requirements.in, bokeh, datashader, matplotlib +psutil==5.9.4 # via -r workers/data_refinery_workers/processors/requirements.in +psycopg2-binary==2.9.5 # via -r workers/data_refinery_workers/processors/requirements.in +pycparser==2.21 # via cffi +pyct==0.5.0 # via colorcet, datashader, panel +pyparsing==3.0.9 # via matplotlib +python-dateutil==2.8.2 # via botocore, datashape, matplotlib, pandas +pytz==2022.7.1 # via django, pandas, rpy2 +pytz-deprecation-shim==0.1.0.post0 # via tzlocal +pyviz-comms==2.2.1 # via holoviews, panel +pyyaml==6.0 # via -r workers/data_refinery_workers/processors/requirements.in, bokeh, dask, vcrpy +requests==2.28.2 # via -r workers/data_refinery_workers/processors/requirements.in, coveralls, datashader, panel +retrying==1.3.4 # via -r workers/data_refinery_workers/processors/requirements.in +rpy2===3.4.5 # via -r workers/data_refinery_workers/processors/requirements.in +s3transfer==0.6.0 # via boto3 +scikit-learn==1.2.1 # via -r workers/data_refinery_workers/processors/requirements.in +scipy==1.10.0 # via -r workers/data_refinery_workers/processors/requirements.in, datashader, scikit-learn +selenium==3.141.0 # via -r workers/data_refinery_workers/processors/requirements.in +simplejson==3.18.3 # via -r workers/data_refinery_workers/processors/requirements.in +six==1.16.0 # via bleach, multipledispatch, python-dateutil, retrying, vcrpy +sqlparse==0.4.3 # via -r workers/data_refinery_workers/processors/requirements.in, django +sympy==1.11.1 # via -r workers/data_refinery_workers/processors/requirements.in +threadpoolctl==3.1.0 # via scikit-learn +toolz==0.12.0 # via dask, datashader, partd +tornado==6.2 # via bokeh +tqdm==4.64.1 # via panel +typing-extensions==4.5.0 # via bokeh, django-computedfields, panel +tzdata==2022.7 # via pytz-deprecation-shim +tzlocal==4.2 # via -r workers/data_refinery_workers/processors/requirements.in, rpy2 +unicodecsv==0.14.1 # via -r workers/data_refinery_workers/processors/requirements.in +untangle==1.2.1 # via -r workers/data_refinery_workers/processors/requirements.in +urllib3==1.26.14 # via botocore, requests, selenium +vcrpy==4.1.1 # via -r workers/data_refinery_workers/processors/requirements.in +webencodings==0.5.1 # via bleach +wrapt==1.14.1 # via vcrpy +xarray==0.16.2 # via -r workers/data_refinery_workers/processors/requirements.in, datashader +yarl==1.8.2 # via vcrpy +zipp==3.13.0 # via importlib-metadata, importlib-resources # The following packages are considered to be unsafe in a requirements file: # setuptools diff --git a/workers/run_tests.sh b/workers/run_tests.sh index a750b502a..66029bb5e 100755 --- a/workers/run_tests.sh +++ b/workers/run_tests.sh @@ -566,8 +566,9 @@ for image in $worker_images; do --env-file workers/environments/test \ --env AWS_ACCESS_KEY_ID \ --env AWS_SECRET_ACCESS_KEY \ - --volume "$volume_directory":/home/user/data_store \ --memory=5G \ + --platform linux/amd64 \ + --volume "$volume_directory":/home/user/data_store \ "$image_name" bash -c "$test_command" fi done diff --git a/workers/tests/processors/test_compendia.py b/workers/tests/processors/test_compendia.py index 85373a6a7..a9abfeda0 100644 --- a/workers/tests/processors/test_compendia.py +++ b/workers/tests/processors/test_compendia.py @@ -146,7 +146,7 @@ def test_create_compendia(self): # Because one of the samples is filtered out, there will be too few # remaining samples to smash together, so we expect this job to fail. - self.assertFailed(job, "k must be between 1 and min(A.shape)") + self.assertFailed(job, "`k` must be an integer satisfying `0 < k < min(A.shape)`") # check that sample with no computed file was skipped self.assertTrue("GSM1487222" in final_context["filtered_samples"]) diff --git a/workers/tests/processors/test_illumina.py b/workers/tests/processors/test_illumina.py index 44e568daa..4b3ee1164 100644 --- a/workers/tests/processors/test_illumina.py +++ b/workers/tests/processors/test_illumina.py @@ -7,7 +7,7 @@ from django.test import TestCase, tag -from tests import utils +from tests import utils as test_utils from data_refinery_common.enums import PipelineEnum from data_refinery_common.models import ( @@ -151,7 +151,7 @@ def _try_sanitizing_file(file: str) -> str: ] -class IlluminaToPCLTestCase(TestCase, testing_utils.ProcessorJobTestCaseMixin): +class IlluminaToPCLTestCase(TestCase, test_utils.ProcessorJobTestCaseMixin): @tag("illumina") def test_illumina_to_pcl(self): """Most basic Illumina to PCL test""" From 93825fb7dcda0a4a70c9a0c05e2a1cfca6e2e970 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 13 Apr 2023 15:47:10 -0700 Subject: [PATCH 101/220] Address review comments. --- .../surveyor/array_express.py | 67 ++++++++++--------- .../test_update_experiment_metadata.py | 2 +- 2 files changed, 37 insertions(+), 32 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/array_express.py b/foreman/data_refinery_foreman/surveyor/array_express.py index e99cb5863..ba11d38f4 100644 --- a/foreman/data_refinery_foreman/surveyor/array_express.py +++ b/foreman/data_refinery_foreman/surveyor/array_express.py @@ -1,4 +1,5 @@ from datetime import datetime +from itertools import chain from typing import Dict, List, Tuple from data_refinery_common.enums import Downloaders @@ -52,36 +53,37 @@ def _parse_api_info_response(data: Dict) -> Dict: @classmethod def _parse_api_data_response(cls, data: Dict) -> Dict: parsed_data = {} - for attribute in data["section"]["attributes"]: - attribute_name = attribute["name"] - if attribute_name not in {"Description", "Organism", "Title"}: - continue - parsed_data[attribute_name.lower()] = attribute["value"] - - for subsection in data["section"]["subsections"]: - for subsection_entry in subsection: - # Protocols. - if isinstance(subsection_entry, dict) and subsection_entry["type"] == "Protocols": - if "protocols" not in parsed_data: - parsed_data["protocols"] = [] - - protocol = {} - for attribute in subsection_entry["attributes"]: - if "value" not in attribute: - continue - protocol[attribute["name"].lower()] = attribute["value"] - if protocol: - parsed_data["protocols"].append(protocol) - - # Array Designs. - elif subsection["type"] == "Assays and Data": - array_designs = [] - for assays_data_subsection in subsection["subsections"]: - if assays_data_subsection["type"] == "Array Designs": - for link in assays_data_subsection["links"]: - for link_entry in link: - array_designs.append(link_entry["url"]) - parsed_data["array_designs"] = array_designs + + # Filter for supported section attributes. + attribute_filter = lambda attr: attr["name"] in { + "Description", + "Organism", + "Title", + } + attributes = data["section"]["attributes"] + for attribute in filter(attribute_filter, attributes): + parsed_data[attribute["name"].lower()] = attribute["value"] + + # Protocols. + parsed_data["protocols"] = [] + protocol_filter = lambda entry: isinstance(entry, dict) and entry["type"] == "Protocols" + value_defined_filter = lambda attr: "value" in attr + subsections = data["section"]["subsections"] + for subsection in filter(protocol_filter, chain.from_iterable(subsections)): + # Protocols. + protocol = {} + for attribute in filter(value_defined_filter, subsection["attributes"]): + protocol[attribute["name"].lower()] = attribute["value"] + parsed_data["protocols"].append(protocol) + + # Array Designs. + parsed_data["array_designs"] = [] + assay_filter = lambda entry: isinstance(entry, list) and entry["type"] == "Assays and Data" + is_design = lambda entry: entry["type"] == "Array Designs" + for subsection in filter(assay_filter, chain.from_iterable(subsections)): + for assays_data_subsection in filter(is_design, subsection): + links = chain.from_iterable(assays_data_subsection["links"]) + parsed_data["array_designs"].extend((link["url"] for link in links)) return parsed_data @@ -544,7 +546,10 @@ def create_samples_from_api(self, experiment: Experiment, platform_dict: Dict) - sample_source_name = sample_data["source"].get("name", "") sample_assay_name = sample_data["assay"].get("name", "") sample_accession_code = self.determine_sample_accession( - experiment.accession_code, sample_source_name, sample_assay_name, filename + experiment.accession_code, + sample_source_name, + sample_assay_name, + filename, ) # Figure out the Organism for this sample diff --git a/foreman/tests/foreman/management/commands/test_update_experiment_metadata.py b/foreman/tests/foreman/management/commands/test_update_experiment_metadata.py index 2b2239f95..5d9f5e233 100644 --- a/foreman/tests/foreman/management/commands/test_update_experiment_metadata.py +++ b/foreman/tests/foreman/management/commands/test_update_experiment_metadata.py @@ -120,7 +120,7 @@ def test_array_express_experiment_missing_metadata(self): command.handle() # Test that the title was fixed. - experiment = Experiment.objects.get_or_create(accession_code=experiment.accession_code)[0] + experiment, _ = Experiment.objects.get_or_create(accession_code=experiment.accession_code) self.assertNotEqual( experiment.title, SurveyTestCase.BAD_TITLE, From 60f2e77fa95178cbf0eedefb23f0f00012d1e83f Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 14 Apr 2023 07:55:54 -0700 Subject: [PATCH 102/220] Add refine.bio GPG signing key. --- keys/ark.gpg | 13 +++++++++++++ 1 file changed, 13 insertions(+) create mode 100644 keys/ark.gpg diff --git a/keys/ark.gpg b/keys/ark.gpg new file mode 100644 index 000000000..17a03d699 --- /dev/null +++ b/keys/ark.gpg @@ -0,0 +1,13 @@ +-----BEGIN PGP PUBLIC KEY BLOCK----- + +mDMEYqzflxYJKwYBBAHaRw8BAQdAc8iFPchxuORjMwIgB5xxbxf+6u/MYkspzZ1I +XE6elXO0JEFya2FkaWkgWWFrb3ZldHMgPGFya0BjY2RhdGFsYWIub3JnPoiSBBMW +CgA7FiEE/ADSxSy4ejoh6Buz76k1vgallBwFAmKs35cCGwMFCwkIBwICIgIGFQoJ +CAsCBBYCAwECHgcCF4AACgkQ76k1vgallBwuwwEA6OJH2iLUAnCnLrBF7QywzC8Z +MUtCpMx5DT21F7nGeVIA+OIdnzn9P3dbm10DBBCKJtEdCdTpgc/w1E7upfxCTA64 +OARirN+XEgorBgEEAZdVAQUBAQdAl3T9RxncqkjvvhVHwCjGzG8GL88vTN6PUh80 +9O3v8iUDAQgHiHgEGBYKACAWIQT8ANLFLLh6OiHoG7PvqTW+BqWUHAUCYqzflwIb +DAAKCRDvqTW+BqWUHAImAQCyxRNjUG7omTXFfGbwYlaz6nLk7y1f1aK1N7Dw/wKJ +LwEAkFTPSjOZeqGPL7MF940Uh49SgnEv+7EEhHBT9vI6uwA= +=SqPM +-----END PGP PUBLIC KEY BLOCK----- From 0acdec99901f0a71e2877df235724a213bc1df6b Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 14 Apr 2023 08:00:05 -0700 Subject: [PATCH 103/220] Clean up unused keys. --- keys/casey.gpg | 52 ------------------------------------------------- keys/dongbo.gpg | 50 ----------------------------------------------- keys/kurt.gpg | 52 ------------------------------------------------- 3 files changed, 154 deletions(-) delete mode 100644 keys/casey.gpg delete mode 100644 keys/dongbo.gpg delete mode 100644 keys/kurt.gpg diff --git a/keys/casey.gpg b/keys/casey.gpg deleted file mode 100644 index 4e4d318f5..000000000 --- a/keys/casey.gpg +++ /dev/null @@ -1,52 +0,0 @@ ------BEGIN PGP PUBLIC KEY BLOCK----- - -mQINBFur3fcBEACkY6c+kEcg2P+qKd9gDIlTnVfmspKl5VRuXvbvZa8VH3GSXv3f -IZuhR9KX/b+bChq2ryZhLXLpztoGbBBFu/gx7gFrFA6Qg8deO8YwbQfczShp8G2L -0zxHYWkbc4onyS3jcz4hFkjh/Uj6i2C+4v2YS/StisYawNKI7H334O1fJy8eeTgQ -aGKSTRybLOc1kjgNy/AO7vhkSvWZaAyvrActupwaVkEagBW6uEa00xyb1Ji62m+L -MSN8ClGTTYkG+n8C0VtBbAmKloE4xz0IC4sYLIR+6XfyXy285FNXd2FvyLHwnplm -GU7NyFd8Wc+4+DsHOwtFKOxE+1XTbALGXBh36/X6aPtOyeXWHFWQr5GW0GEoPUJV -E/tz7rB8bklN69Jq4+RM9XzFmstAni8OLtZ/Q8IBHLWFzEdSSFEfkFaMwyFr6M3U 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-tUi+Uh8FXKvl+mjzeCuVy+wmWU5bs9EnS5AYSQZXC63GO16RVYiZ/tCopn8gMkNR -cCZfCXV5+XuFuCnSrH/DJMBPJLHp3fg75T2blN9LNcRCySKZMv+y3iI5FRqXFklh -r6v33DQ3wwOnY/Id2D8fBqV8A2hz5Fykc1uANxU7kjiczAzEUalyjgPVNkZma+K1 -SoJCBtm7kl4jD8SioBtPC9ac8JJzzBBUV1ZdJFtmFaSKXZjBxaHdeUxrsk5ngtff -6FVjBx72FY7rsM9hAS/Vv+w/jUDd0iYXGX6I5J8TQA8dfBdyr4biVYV05DHFSuPQ -rSM7tOfMpoSH6G6GTiC5BhgSNBLaj2wRnVVbGdzmuFKQ/n1rSwaiuMiEZLegrPh/ -HbUG -=upP7 ------END PGP PUBLIC KEY BLOCK----- From 39ffb3c5068506fb952d732fa7616fe05f9c0c58 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Thu, 16 Mar 2023 16:45:49 -0400 Subject: [PATCH 104/220] pull salmontools test data from s3 --- workers/run_tests.sh | 19 ++++++++++++------- 1 file changed, 12 insertions(+), 7 deletions(-) diff --git a/workers/run_tests.sh b/workers/run_tests.sh index 66029bb5e..3e03e2ada 100755 --- a/workers/run_tests.sh +++ b/workers/run_tests.sh @@ -77,13 +77,18 @@ if [ -z "$tag" ] || [ "$tag" = "salmon" ]; then rm "$volume_directory"/salmon_tests.tar.gz fi - # Download salmontools test data - rm -rf "$volume_directory"/salmontools/ - git clone https://github.com/dongbohu/salmontools_tests.git "$volume_directory"/salmontools - - # Download tximport test data - rm -rf "$volume_directory"/tximport_test/ - git clone https://github.com/dongbohu/tximport_test.git "$volume_directory"/tximport_test + # salmontools test data + salmontools_test_zip="$test_data_repo/salmontools_test_data.tar.gz" + salmontools_test_dir="$volume_directory/salmontools" + + # Clean the test data directory + rm -rf "$salmontools_test_dir" + mkdir -p "$salmontools_test_dir" + + echo "Downloading 'salmontools' test data..." + wget -q -O "$volume_directory"/salmontools.tar.gz "$salmontools_test_zip" + tar xzf "$volume_directory"/salmontools.tar.gz -C "$volume_directory" + rm "$volume_directory"/salmontools.tar.gz # Make sure data for Salmon test is downloaded from S3. rna_seq_test_raw_dir="$volume_directory/raw/TEST/SALMON" From 276c2d4d89ff595afab50d1f20220d26fa24da7b Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 16 Feb 2023 10:08:15 -0800 Subject: [PATCH 105/220] Address review comments. Fix git unrelated history issue. --- .../commands/post_downloads_summary.py | 196 ++++++++++++------ .../commands/test_post_downloads_summary.py | 128 ++++++++++-- 2 files changed, 237 insertions(+), 87 deletions(-) diff --git a/api/data_refinery_api/management/commands/post_downloads_summary.py b/api/data_refinery_api/management/commands/post_downloads_summary.py index 30a49cb1f..75db2db9b 100644 --- a/api/data_refinery_api/management/commands/post_downloads_summary.py +++ b/api/data_refinery_api/management/commands/post_downloads_summary.py @@ -1,7 +1,9 @@ import datetime +from collections import Counter from django.conf import settings from django.core.management.base import BaseCommand +from django.template.defaultfilters import pluralize from django.utils import timezone import requests @@ -9,109 +11,173 @@ from data_refinery_common.models import Dataset, DatasetAnnotation -def post_downloads_summary(days, channel): - start_time = timezone.now() - datetime.timedelta(days=days) +class Command(BaseCommand): + help = "Post downloads summary to Slack" + def add_arguments(self, parser): + parser.add_argument( + "--channel", + type=str, + default="ccdl-general", + help=("Optional parameter to choose the channel where the message will be posted."), + ) + parser.add_argument( + "--days", + type=int, + default=7, # Default to a week. + help=("Number of days in the past for which to build the stats."), + ) + parser.add_argument( + "--top-countries", + type=int, + default=5, + help=("Number of countries to show in the per country downloads summary."), + ) + + def handle(self, *args, **options): + post_downloads_summary(options["days"], options["channel"], options["top_countries"]) + + +def format_user_data(header, data): + """ + Formats user email, downloads count, location information sorted + by downloads count. + """ + # Allowed overhead for 2 column sorting: downloads count, email. + lines = sorted(data, key=lambda u: u[0].lower()) + lines = [ + f"{email.lower()} | {downloads} download{pluralize(downloads)} from {location}" + for email, downloads, location in sorted(lines, key=lambda u: u[1], reverse=True) + ] + lines.insert(0, header) + + return "\n".join(lines) + + +def get_user_location(ip_address): + """Gets user location information based on their IP address.""" + try: + data = requests.get(f"https://ipapi.co/{ip_address}/json/", timeout=10).json() + # The list of available fields https://ipapi.co/api/#complete-location + return ", ".join((data["city"], data["country_name"])) + except (requests.exceptions.RequestException, KeyError, ValueError): + return ip_address + + +def post_downloads_summary(days, channel, top_countries=5): + """Posts downloads summary to Slack channel.""" + + start_time = timezone.now() - datetime.timedelta(days=days) datasets = Dataset.processed_filtered_objects.filter( created_at__gt=start_time ).prefetch_related("datasetannotation_set") + annotations = DatasetAnnotation.objects.filter(dataset__in=datasets) + users_emails = set(dataset.email_address for dataset in datasets) - annotation_queryset = DatasetAnnotation.objects.filter(dataset__in=datasets) - - # Save the locations permanently, since IP addresses can cycle over time - location_cache = dict() - for annotation in annotation_queryset: + locations = set() + locations_cache = dict() + for annotation in annotations: if "location" not in annotation.data: - ip = annotation.data["ip"] - if ip not in location_cache: - location_cache[ip] = get_ip_location(ip) - annotation.data["location"] = location_cache[ip] + ip_address = annotation.data["ip"] + if ip_address not in locations_cache: + locations_cache[ip_address] = get_user_location(ip_address) + + # Save the locations permanently, since IP addresses can cycle over time. + annotation.data["location"] = locations_cache[ip_address] annotation.save() + locations.add(annotation.data["location"]) + + downloads_per_country = Counter() + downloads_total = 0 + new_users = [] + returning_users = [] + for user_email in users_emails: + user_annotations = annotations.filter(dataset__email_address=user_email) + user_downloads = user_annotations.count() + downloads_total += user_downloads + + user_locations = set() + for user_annotation in user_annotations: + user_locations.add(annotation.data["location"]) + try: + country = user_annotation.data["location"].split(", ")[1] + downloads_per_country.update({country: 1}) + except (IndexError, TypeError): + pass + + user_locations = "; ".join(sorted(user_locations)) + user_data = (user_email, user_downloads, user_locations) - unique_users = list(set(dataset.email_address for dataset in datasets)) - unique_locations = list(set(annotation.data["location"] for annotation in annotation_queryset)) - - new_users = "" - returning_users = "" - total_downloads = 0 - for email in unique_users: - user_annotations = annotation_queryset.filter(dataset__email_address=email) - downloads = user_annotations.count() - total_downloads += downloads - locations = ", ".join( - list(set(annotation.data["location"] for annotation in user_annotations)) - ) is_returning_user = Dataset.processed_filtered_objects.filter( - created_at__lt=start_time, email_address=email + created_at__lt=start_time, email_address=user_email ) - text = "{0} | {1} downloads from {2}\n".format(email, downloads, locations) if is_returning_user: - returning_users += text + returning_users.append(user_data) else: - new_users += text + new_users.append(user_data) - if total_downloads > 0: + if downloads_total: + locations_count = len(locations) + users_emails_count = len(users_emails) fallback_text = ( - f"In the last {days} days, {len(unique_users)} users downloaded {total_downloads}" - f" datasets from {len(unique_locations)} locations." + f"In the last {days} day{pluralize(days)}, {users_emails_count} " + f"user{pluralize(users_emails_count)} downloaded {downloads_total} " + f"dataset{pluralize(downloads_total)} from {locations_count} " + f"location{pluralize(locations_count)}." ) else: - fallback_text = f"There were no downloads in the last {days} days." + fallback_text = f"There were no downloads in the last {days} day{pluralize(days)}." blocks = [ {"type": "section", "text": {"type": "plain_text", "emoji": True, "text": fallback_text}} ] + if new_users: blocks.append( - {"type": "section", "text": {"type": "mrkdwn", "text": "*New users* \n" + new_users}} + { + "type": "section", + "text": {"type": "mrkdwn", "text": format_user_data("*New users*", new_users)}, + } ) + if returning_users: blocks.append( { "type": "section", - "text": {"type": "mrkdwn", "text": "*Returning users* \n" + returning_users}, + "text": { + "type": "mrkdwn", + "text": format_user_data("*Returning users*", returning_users), + }, + } + ) + + if top_countries and downloads_per_country: + countries_count = downloads_per_country.most_common(top_countries) + top_countries = min(top_countries, len(countries_count)) + lines = [f"*Top {top_countries} countr{pluralize(top_countries, 'y,ies')}*"] + # Allowed overhead for 2 column sorting: downloads count, country. + countries_count = sorted(countries_count, key=lambda cc: cc[0]) + countries_count = sorted(countries_count, key=lambda cc: cc[1], reverse=True) + for country, count in countries_count: + lines.append(f"{country}: {count} download{pluralize(count)}") + + blocks.append( + { + "type": "section", + "text": {"type": "mrkdwn", "text": "\n".join(lines)}, } ) - # Post to slack + # Post to Slack. requests.post( settings.ENGAGEMENTBOT_WEBHOOK, json={ "username": "EngagementBot", "icon_emoji": ":halal:", - "channel": "#" + channel, + "channel": f"#{channel}", "text": fallback_text, "blocks": blocks, }, headers={"Content-Type": "application/json"}, timeout=10, ) - - -class Command(BaseCommand): - help = "Post downloads summary to slack" - - def add_arguments(self, parser): - parser.add_argument( - "--days", - type=int, - default=7, # default to a week - help=("Number of days in the past for which to build the stats"), - ) - parser.add_argument( - "--channel", - type=str, - default="ccdl-general", - help=("Optional parameter to choose the channel where the message will be posted."), - ) - - def handle(self, *args, **options): - post_downloads_summary(options["days"], options["channel"]) - - -def get_ip_location(remote_ip): - try: - data = requests.get("https://ipapi.co/" + remote_ip + "/json/", timeout=10).json() - return "{0}, {1}".format(data["city"], data["country_name"]) - except (requests.exceptions.RequestException, ValueError, KeyError): - return remote_ip diff --git a/api/tests/management/commands/test_post_downloads_summary.py b/api/tests/management/commands/test_post_downloads_summary.py index f7b00b898..967986b42 100644 --- a/api/tests/management/commands/test_post_downloads_summary.py +++ b/api/tests/management/commands/test_post_downloads_summary.py @@ -9,51 +9,135 @@ class DownloadsPostTestCase(TestCase): - @patch("data_refinery_common.models.organism.requests.post") + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") def test_post_download_summary_one_download(self, requests_post): dataset = Dataset(email_address="test@gmail.com", is_processed=True) dataset.save() - DatasetAnnotation(dataset=dataset, data={"location": "Testyville"}).save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() - post_downloads_summary(7, "ccdl-general") + post_downloads_summary(7, "ccdl-general-test") + json = requests_post.mock_calls[0][2]["json"] - text = requests_post.mock_calls[0][2]["json"]["text"] - self.assertIn("1 users downloaded 1 datasets from 1 locations.", text) + self.assertEqual( + "In the last 7 days, 1 user downloaded 1 dataset from 1 location.", json["text"] + ) + self.assertEqual( + "\n".join(("*New users*", "test@gmail.com | 1 download from Testyville, TSA")), + json["blocks"][1]["text"]["text"], + ) + self.assertEqual( + "\n".join(("*Top 1 country*", "TSA: 1 download")), json["blocks"][2]["text"]["text"] + ) - @patch("data_refinery_common.models.organism.requests.post") + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") def test_post_download_summary_two_downloads(self, requests_post): dataset = Dataset(email_address="test@gmail.com", is_processed=True) dataset.save() - DatasetAnnotation(dataset=dataset, data={"location": "Testyville"}).save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() + + dataset = Dataset(email_address="test@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() + dataset = Dataset(email_address="test2@gmail.com", is_processed=True) dataset.save() - DatasetAnnotation(dataset=dataset, data={"location": "Not Testyville"}).save() + DatasetAnnotation(dataset=dataset, data={"location": "Not Testyville, Not TSA"}).save() - post_downloads_summary(7, "ccdl-general") + post_downloads_summary(7, "ccdl-general-test") + json = requests_post.mock_calls[0][2]["json"] - text = requests_post.mock_calls[0][2]["json"]["text"] - self.assertIn("2 users downloaded 2 datasets from 2 locations.", text) + self.assertEqual( + "In the last 7 days, 2 users downloaded 3 datasets from 2 locations.", json["text"] + ) + self.assertEqual( + "\n".join( + ( + "*New users*", + "test@gmail.com | 2 downloads from Testyville, TSA", + "test2@gmail.com | 1 download from Not Testyville, Not TSA", + ) + ), + json["blocks"][1]["text"]["text"], + ) + self.assertEqual( + "\n".join(("*Top 2 countries*", "TSA: 2 downloads", "Not TSA: 1 download")), + json["blocks"][2]["text"]["text"], + ) - @patch("data_refinery_common.models.organism.requests.post") + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") + def test_post_download_summary_ordering(self, requests_post): + for _ in range(2): + dataset = Dataset(email_address="ATEST2@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Not Testyville, Not TSA"}).save() + + for _ in range(2): + dataset = Dataset(email_address="TEST@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() + + dataset = Dataset(email_address="TEST3@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Testbec City, Tanada"}).save() + + for _ in range(3): + dataset = Dataset(email_address="WEST@gmail.com", is_processed=True) + dataset.save() + DatasetAnnotation(dataset=dataset, data={"location": "Westyville, Texico"}).save() + + post_downloads_summary(7, "ccdl-general-test") + json = requests_post.mock_calls[0][2]["json"] + + self.assertEqual( + "In the last 7 days, 4 users downloaded 8 datasets from 4 locations.", json["text"] + ) + self.assertEqual( + "\n".join( + ( + "*New users*", + "west@gmail.com | 3 downloads from Westyville, Texico", + "atest2@gmail.com | 2 downloads from Not Testyville, Not TSA", + "test@gmail.com | 2 downloads from Testyville, TSA", + "test3@gmail.com | 1 download from Testbec City, Tanada", + ) + ), + json["blocks"][1]["text"]["text"], + ) + self.assertEqual( + "\n".join( + ( + "*Top 4 countries*", + "Texico: 3 downloads", + "Not TSA: 2 downloads", + "TSA: 2 downloads", + "Tanada: 1 download", + ) + ), + json["blocks"][2]["text"]["text"], + ) + + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") def test_post_download_summary_filtered(self, requests_post): dataset = Dataset(email_address="test@gmail.com", is_processed=True) dataset.save() - DatasetAnnotation(dataset=dataset, data={"location": "Testyville"}).save() + DatasetAnnotation(dataset=dataset, data={"location": "Testyville, TSA"}).save() + last_week = timezone.now() - timedelta(days=8) dataset = Dataset(created_at=last_week, email_address="test2@gmail.com", is_processed=True) dataset.save() DatasetAnnotation( - created_at=last_week, dataset=dataset, data={"location": "Not Testyville"} + created_at=last_week, dataset=dataset, data={"location": "Testyville, TSA"} ).save() - post_downloads_summary(7, "ccdl-general") - - text = requests_post.mock_calls[0][2]["json"]["text"] - self.assertIn("1 users downloaded 1 datasets from 1 locations.", text) + post_downloads_summary(7, "ccdl-general-test") + json = requests_post.mock_calls[0][2]["json"] + self.assertEqual( + "In the last 7 days, 1 user downloaded 1 dataset from 1 location.", json["text"] + ) - @patch("data_refinery_common.models.organism.requests.post") + @patch("data_refinery_api.management.commands.post_downloads_summary.requests.post") def test_empty_post_download_summary(self, requests_post): - post_downloads_summary(7, "ccdl-general") + post_downloads_summary(7, "ccdl-general-test") - text = requests_post.mock_calls[0][2]["json"]["text"] - self.assertIn("no downloads", text) + json = requests_post.mock_calls[0][2]["json"] + self.assertEqual("There were no downloads in the last 7 days.", json["text"]) From 57b3b525e0f1a7a17734d492e4f2a1edf0e0a4f8 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 16 Feb 2023 10:15:47 -0800 Subject: [PATCH 106/220] Fix tests. --- .../management/commands/post_downloads_summary.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/api/data_refinery_api/management/commands/post_downloads_summary.py b/api/data_refinery_api/management/commands/post_downloads_summary.py index 75db2db9b..f43650cd5 100644 --- a/api/data_refinery_api/management/commands/post_downloads_summary.py +++ b/api/data_refinery_api/management/commands/post_downloads_summary.py @@ -98,7 +98,7 @@ def post_downloads_summary(days, channel, top_countries=5): user_locations = set() for user_annotation in user_annotations: - user_locations.add(annotation.data["location"]) + user_locations.add(user_annotation.data["location"]) try: country = user_annotation.data["location"].split(", ")[1] downloads_per_country.update({country: 1}) From b524001ca9dbbc7c16212340b115d3c1e45d8e14 Mon Sep 17 00:00:00 2001 From: ark Date: Thu, 5 May 2022 12:03:20 -0700 Subject: [PATCH 107/220] Get rid of wildcard imports. --- .../import_external_sample_attributes.py | 2 +- .../import_external_sample_keywords.py | 2 +- .../management/commands/clear_database.py | 21 ++++++++++++++++++- .../commands/correct_affy_samples.py | 2 +- 4 files changed, 23 insertions(+), 4 deletions(-) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_attributes.py b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_attributes.py index c22a3f354..def44fbdd 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_attributes.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_attributes.py @@ -23,7 +23,7 @@ import botocore from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models import * +from data_refinery_common.models import Contribution, OntologyTerm, Sample, SampleAttribute from data_refinery_common.utils import parse_s3_url logger = get_and_configure_logger(__name__) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py index 8b1d0c93a..c3b84375a 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/import_external_sample_keywords.py @@ -25,7 +25,7 @@ import botocore from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models import * +from data_refinery_common.models import Contribution, OntologyTerm, Sample, SampleKeyword from data_refinery_common.utils import parse_s3_url logger = get_and_configure_logger(__name__) diff --git a/foreman/data_refinery_foreman/surveyor/management/commands/clear_database.py b/foreman/data_refinery_foreman/surveyor/management/commands/clear_database.py index 81ea94c7e..a1518c6ae 100644 --- a/foreman/data_refinery_foreman/surveyor/management/commands/clear_database.py +++ b/foreman/data_refinery_foreman/surveyor/management/commands/clear_database.py @@ -4,7 +4,26 @@ from django.core.management.base import BaseCommand -from data_refinery_common.models import * +from data_refinery_common.models import ( + ComputationalResult, + ComputationalResultAnnotation, + ComputedFile, + DownloaderJob, + DownloaderJobOriginalFileAssociation, + Experiment, + ExperimentAnnotation, + ExperimentSampleAssociation, + Organism, + OrganismIndex, + OriginalFile, + ProcessorJob, + ProcessorJobOriginalFileAssociation, + Sample, + SampleAnnotation, + SampleResultAssociation, + SurveyJob, + SurveyJobKeyValue, +) class Command(BaseCommand): diff --git a/workers/data_refinery_workers/processors/management/commands/correct_affy_samples.py b/workers/data_refinery_workers/processors/management/commands/correct_affy_samples.py index 8f5567b66..7de449438 100644 --- a/workers/data_refinery_workers/processors/management/commands/correct_affy_samples.py +++ b/workers/data_refinery_workers/processors/management/commands/correct_affy_samples.py @@ -22,7 +22,7 @@ from rpy2.rinterface_lib.embedded import RRuntimeError from data_refinery_common.logging import get_and_configure_logger -from data_refinery_common.models import * +from data_refinery_common.models import Sample from data_refinery_common.utils import get_env_variable, get_readable_affymetrix_names logger = get_and_configure_logger(__name__) From 826322842ce7a2989ed6767818e2fc7bd8f61d7b Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 17 Apr 2023 13:43:46 -0400 Subject: [PATCH 108/220] Fix test_tximport.R path --- workers/tests/processors/test_salmon.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workers/tests/processors/test_salmon.py b/workers/tests/processors/test_salmon.py index e2744668b..c97bdaa6b 100644 --- a/workers/tests/processors/test_salmon.py +++ b/workers/tests/processors/test_salmon.py @@ -473,7 +473,7 @@ def test_salmon_quant_two_samples_single_read(self): cmd_tokens = [ "/usr/bin/Rscript", "--vanilla", - "/home/user/data_refinery_workers/processors/test_tximport.R", + "/home/user/tests/processors/test_tximport.R", "--txi_out", rds_file_path, "--gene2txmap", From 9a693daee245d6c1a34d2d02f26dbfab6a9459cc Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 17 Apr 2023 12:20:02 -0700 Subject: [PATCH 109/220] Update refine.bio public key. --- infrastructure/security.tf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/infrastructure/security.tf b/infrastructure/security.tf index 517ecc2cc..cf2466e9c 100644 --- a/infrastructure/security.tf +++ b/infrastructure/security.tf @@ -5,7 +5,7 @@ # debugging. Long term we may want to remove this entirely. resource "aws_key_pair" "data_refinery" { key_name = "data-refinery-key-${var.user}-${var.stage}" - public_key = "ssh-rsa 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" + public_key = "ssh-ed25519 AAAAC3NzaC1lZDI1NTE5AAAAIEAsg062YxUo1ti8tT2gR63JVHpLI5jkxkADwEeVb79O" } From a1deca63126946656350a1d902d1f5f8ce0959f0 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 17 Apr 2023 12:47:04 -0700 Subject: [PATCH 110/220] Fix keys path. --- .github/scripts/verify_tag.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/scripts/verify_tag.sh b/.github/scripts/verify_tag.sh index b0c0387d3..afeea0c2c 100755 --- a/.github/scripts/verify_tag.sh +++ b/.github/scripts/verify_tag.sh @@ -6,7 +6,7 @@ cd ~/refinebio || exit for key in keys/*; do - gpg --import "keys/$key" + gpg --import "$key" done # If it is not a good key then the exit code is 1, which will cause From 6b09596161929c05d71c9449d8daec8f19bcef24 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 18 Apr 2023 11:13:03 -0700 Subject: [PATCH 111/220] Pin setuptools to version 65.7 --- api/dockerfiles/Dockerfile.api_local | 1 + api/dockerfiles/Dockerfile.api_production | 1 + common/dockerfiles/Dockerfile.base | 3 ++- workers/dockerfiles/Dockerfile.compendia | 3 ++- 4 files changed, 6 insertions(+), 2 deletions(-) diff --git a/api/dockerfiles/Dockerfile.api_local b/api/dockerfiles/Dockerfile.api_local index 2018cd59b..a83e98bd8 100644 --- a/api/dockerfiles/Dockerfile.api_local +++ b/api/dockerfiles/Dockerfile.api_local @@ -15,6 +15,7 @@ WORKDIR /home/user COPY api/requirements.txt . RUN pip install --upgrade pip && \ + pip install setuptools==65.7 && \ pip install --ignore-installed -r requirements.txt && \ rm -r /root/.cache diff --git a/api/dockerfiles/Dockerfile.api_production b/api/dockerfiles/Dockerfile.api_production index 37184be03..bebf793c8 100644 --- a/api/dockerfiles/Dockerfile.api_production +++ b/api/dockerfiles/Dockerfile.api_production @@ -15,6 +15,7 @@ WORKDIR /home/user COPY api/requirements.txt . RUN pip install --upgrade pip && \ + pip install setuptools==65.7 && \ pip install --ignore-installed -r requirements.txt && \ pip install --ignore-installed uwsgi && \ rm -r /root/.cache diff --git a/common/dockerfiles/Dockerfile.base b/common/dockerfiles/Dockerfile.base index 13987e894..5119781b7 100644 --- a/common/dockerfiles/Dockerfile.base +++ b/common/dockerfiles/Dockerfile.base @@ -59,7 +59,8 @@ RUN apt-get update && \ ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ update-alternatives --install \ /usr/bin/python3 python3 /usr/bin/python3.8 1 && \ - pip3 install --upgrade pip setuptools && \ + pip3 install --upgrade pip && \ + pip3 install setuptools==65.7 && \ groupadd user && \ useradd --create-home --home-dir /home/user/ -g user user && \ chown -R user /home/user/ diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 7b8240b78..af8d43483 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -59,7 +59,8 @@ RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ update-alternatives --install /usr/bin/python3 python3 \ /usr/bin/python3.8 1 && \ - pip3 install --upgrade pip setuptools && \ + pip3 install --upgrade pip && \ + pip3 install setuptools==65.7 && \ groupadd user && \ useradd --create-home --home-dir /home/user -g user user From 1a892463d481d8121258aa461fdd630429505a8e Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 18 Apr 2023 15:35:07 -0700 Subject: [PATCH 112/220] Revert setuptools version pinning. --- api/dockerfiles/Dockerfile.api_local | 1 - api/dockerfiles/Dockerfile.api_production | 1 - common/dockerfiles/Dockerfile.base | 3 +-- workers/dockerfiles/Dockerfile.compendia | 3 +-- 4 files changed, 2 insertions(+), 6 deletions(-) diff --git a/api/dockerfiles/Dockerfile.api_local b/api/dockerfiles/Dockerfile.api_local index a83e98bd8..2018cd59b 100644 --- a/api/dockerfiles/Dockerfile.api_local +++ b/api/dockerfiles/Dockerfile.api_local @@ -15,7 +15,6 @@ WORKDIR /home/user COPY api/requirements.txt . RUN pip install --upgrade pip && \ - pip install setuptools==65.7 && \ pip install --ignore-installed -r requirements.txt && \ rm -r /root/.cache diff --git a/api/dockerfiles/Dockerfile.api_production b/api/dockerfiles/Dockerfile.api_production index bebf793c8..37184be03 100644 --- a/api/dockerfiles/Dockerfile.api_production +++ b/api/dockerfiles/Dockerfile.api_production @@ -15,7 +15,6 @@ WORKDIR /home/user COPY api/requirements.txt . RUN pip install --upgrade pip && \ - pip install setuptools==65.7 && \ pip install --ignore-installed -r requirements.txt && \ pip install --ignore-installed uwsgi && \ rm -r /root/.cache diff --git a/common/dockerfiles/Dockerfile.base b/common/dockerfiles/Dockerfile.base index 5119781b7..13987e894 100644 --- a/common/dockerfiles/Dockerfile.base +++ b/common/dockerfiles/Dockerfile.base @@ -59,8 +59,7 @@ RUN apt-get update && \ ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ update-alternatives --install \ /usr/bin/python3 python3 /usr/bin/python3.8 1 && \ - pip3 install --upgrade pip && \ - pip3 install setuptools==65.7 && \ + pip3 install --upgrade pip setuptools && \ groupadd user && \ useradd --create-home --home-dir /home/user/ -g user user && \ chown -R user /home/user/ diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index af8d43483..7b8240b78 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -59,8 +59,7 @@ RUN echo debconf apt-fast/maxdownloads string 16 | debconf-set-selections && \ ln -s /usr/bin/llvm-config-10 /usr/bin/llvm-config && \ update-alternatives --install /usr/bin/python3 python3 \ /usr/bin/python3.8 1 && \ - pip3 install --upgrade pip && \ - pip3 install setuptools==65.7 && \ + pip3 install --upgrade pip setuptools && \ groupadd user && \ useradd --create-home --home-dir /home/user -g user user From 5c6cf98ebecdfadb5a9cda969f2a2de72eb05e2f Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 18 Apr 2023 15:55:45 -0700 Subject: [PATCH 113/220] Change common package version format. --- common/setup.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/common/setup.py b/common/setup.py index 64d62624c..78339b2e1 100644 --- a/common/setup.py +++ b/common/setup.py @@ -8,7 +8,7 @@ VERSION_FILE = "version" try: with open(VERSION_FILE, "rt") as version_file: - version_string = version_file.read().strip() + version_string = version_file.read().strip().split("-")[0] except OSError: print( "Cannot read version to determine System Version." From 568d820a47c0a17d1939f52ac57e8e295c945b94 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 19 Apr 2023 18:34:27 -0700 Subject: [PATCH 114/220] Fix `volume_directory` permissions --- api/run_tests.sh | 2 +- common/run_tests.sh | 2 +- foreman/run_end_to_end_tests.sh | 2 +- foreman/run_management_command.sh | 2 +- foreman/run_tests.sh | 2 +- foreman/test_survey.sh | 2 +- scripts/run_manage.sh | 2 +- scripts/run_shell.sh | 2 +- workers/run_command.sh | 4 +--- workers/run_job.sh | 3 +-- 10 files changed, 10 insertions(+), 13 deletions(-) diff --git a/api/run_tests.sh b/api/run_tests.sh index fedf4cbbe..f81309fbd 100755 --- a/api/run_tests.sh +++ b/api/run_tests.sh @@ -28,8 +28,8 @@ project_root=$(pwd) # "cd .." called above volume_directory="$project_root/test_volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" ./scripts/prepare_image.sh -i api_local -s api diff --git a/common/run_tests.sh b/common/run_tests.sh index dfed8defd..51c564270 100755 --- a/common/run_tests.sh +++ b/common/run_tests.sh @@ -22,8 +22,8 @@ project_root=$(pwd) # "cd .." called above volume_directory="$project_root/test_volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" ./scripts/prepare_image.sh -i common_tests -s common diff --git a/foreman/run_end_to_end_tests.sh b/foreman/run_end_to_end_tests.sh index 03e3fd4b8..01960aa84 100755 --- a/foreman/run_end_to_end_tests.sh +++ b/foreman/run_end_to_end_tests.sh @@ -15,8 +15,8 @@ project_root=$(cd .. && pwd) volume_directory="$project_root/test_volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" test_data_repo="https://s3.amazonaws.com/data-refinery-test-assets" reference_file_dir="$volume_directory/reference/" diff --git a/foreman/run_management_command.sh b/foreman/run_management_command.sh index 08231665a..a9e4ce101 100755 --- a/foreman/run_management_command.sh +++ b/foreman/run_management_command.sh @@ -15,8 +15,8 @@ cd .. volume_directory="$script_directory/volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" ./scripts/prepare_image.sh -i foreman -s foreman diff --git a/foreman/run_tests.sh b/foreman/run_tests.sh index e35df351b..8edbdc56f 100755 --- a/foreman/run_tests.sh +++ b/foreman/run_tests.sh @@ -15,8 +15,8 @@ project_root=$(cd .. && pwd) volume_directory="$project_root/test_volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" # However in order to give Docker access to all the code we have to # move up a level diff --git a/foreman/test_survey.sh b/foreman/test_survey.sh index 7a4016573..7df91a3ae 100755 --- a/foreman/test_survey.sh +++ b/foreman/test_survey.sh @@ -59,8 +59,8 @@ project_root=$(cd .. && pwd) volume_directory="$project_root/test_volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" # However in order to give Docker access to all the code we have to # move up a level diff --git a/scripts/run_manage.sh b/scripts/run_manage.sh index 9ccb4761d..51cc2f550 100755 --- a/scripts/run_manage.sh +++ b/scripts/run_manage.sh @@ -76,8 +76,8 @@ fi volume_directory="$script_directory/../api/volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" docker build -t dr_shell -f "$service/dockerfiles/Dockerfile.$image" . diff --git a/scripts/run_shell.sh b/scripts/run_shell.sh index d5be913ad..cfb60d00c 100755 --- a/scripts/run_shell.sh +++ b/scripts/run_shell.sh @@ -25,8 +25,8 @@ cd .. volume_directory="$script_directory/../foreman/volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" docker build -t dr_shell -f foreman/dockerfiles/Dockerfile.foreman . diff --git a/workers/run_command.sh b/workers/run_command.sh index 0edfd614f..da50660b1 100755 --- a/workers/run_command.sh +++ b/workers/run_command.sh @@ -43,14 +43,12 @@ fi volume_directory="$script_directory/volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" . ./scripts/common.sh DB_HOST_IP=$(get_docker_db_ip_address) -chmod -R a+rwX "$volume_directory" - ./scripts/prepare_image.sh -i "$image" -s workers image_name=ccdlstaging/dr_"$image" diff --git a/workers/run_job.sh b/workers/run_job.sh index 081b94646..7342103bc 100755 --- a/workers/run_job.sh +++ b/workers/run_job.sh @@ -78,11 +78,10 @@ else fi volume_directory="$script_directory/volume" - if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" - chmod -R a+rwX "$volume_directory" fi +chmod -R a+rwX "$volume_directory" source scripts/common.sh DB_HOST_IP=$(get_docker_db_ip_address) From f4a59f151187ef659a064976457a46e7480460e0 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 19 Apr 2023 18:39:59 -0700 Subject: [PATCH 115/220] Remove default profile Fix `failed to get shared config profile, default` error. --- infrastructure/batch/job_queue.tf | 1 - infrastructure/networking.tf | 1 - 2 files changed, 2 deletions(-) diff --git a/infrastructure/batch/job_queue.tf b/infrastructure/batch/job_queue.tf index e8716b3a3..e67dc49bc 100644 --- a/infrastructure/batch/job_queue.tf +++ b/infrastructure/batch/job_queue.tf @@ -1,6 +1,5 @@ # AWS Batch setup provider "aws" { - profile = "default" region = "us-east-1" } diff --git a/infrastructure/networking.tf b/infrastructure/networking.tf index 77c87189f..865debe73 100644 --- a/infrastructure/networking.tf +++ b/infrastructure/networking.tf @@ -2,7 +2,6 @@ # related to networking. provider "aws" { - profile = "default" region = var.region } From 732cd0225a68d1a7555426b5c91f20dc948b542a Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 20 Apr 2023 09:21:44 -0700 Subject: [PATCH 116/220] Migrate deprecated `set-output` command --- .github/workflows/config.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 74113e557..be3a36d7f 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -478,7 +478,7 @@ jobs: name: Set the $CI_TAG environment variable run: | source scripts/common.sh - echo "::set-output name=branch::$(get_master_or_dev ${GITHUB_REF#refs/tags/})" + echo "branch=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_OUTPUT deploy: if: startsWith(github.ref, 'refs/tags/v') && ! endsWith(github.ref, '-hotfix') From fb5dfa91303e8b3d1554028a0e5290f8fd52bc2e Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 20 Apr 2023 09:40:07 -0700 Subject: [PATCH 117/220] Update Foreman end2end test runner location --- foreman/run_end_to_end_tests.sh | 18 +++++++++--------- 1 file changed, 9 insertions(+), 9 deletions(-) diff --git a/foreman/run_end_to_end_tests.sh b/foreman/run_end_to_end_tests.sh index 01960aa84..554068980 100755 --- a/foreman/run_end_to_end_tests.sh +++ b/foreman/run_end_to_end_tests.sh @@ -43,12 +43,12 @@ done < ../infrastructure/prod_env # locally, and when running locally `prepare_image.sh` makes the forman # ccdlstaging/dr_foreman. docker run -t \ - --env-file ../infrastructure/prod_env \ - --env RUNNING_IN_CLOUD=False \ - --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ - --env JOB_DEFINITION_PREFIX="$USER_$STAGE_" \ - --env REFINEBIO_BASE_URL="http://$API_HOST/v1/" \ - --env DJANGO_SECRET_KEY="TEST_KEY_FOR_DEV" \ - --volume "$volume_directory":/home/user/data_store \ - --volume "$HOME/.aws":/home/user/.aws \ - ccdlstaging/dr_foreman python3 manage.py test --no-input --parallel=2 --testrunner='data_refinery_foreman.test_runner.NoDbTestRunner' data_refinery_foreman.foreman.test_end_to_end + --env-file ../infrastructure/prod_env \ + --env RUNNING_IN_CLOUD=False \ + --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ + --env JOB_DEFINITION_PREFIX="$USER_$STAGE_" \ + --env REFINEBIO_BASE_URL="http://$API_HOST/v1/" \ + --env DJANGO_SECRET_KEY="TEST_KEY_FOR_DEV" \ + --volume "$volume_directory":/home/user/data_store \ + --volume "$HOME/.aws":/home/user/.aws \ + ccdlstaging/dr_foreman python3 manage.py test --no-input --parallel=2 --testrunner='tests.test_runner.NoDbTestRunner' tests.foreman.test_end_to_end From a80a5c2512dea34597908822017f92379974e134 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 20 Apr 2023 11:54:35 -0700 Subject: [PATCH 118/220] Add a temporary workaround for failing end2end test --- foreman/tests/foreman/test_end_to_end.py | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/foreman/tests/foreman/test_end_to_end.py b/foreman/tests/foreman/test_end_to_end.py index d176029c2..918db83d7 100644 --- a/foreman/tests/foreman/test_end_to_end.py +++ b/foreman/tests/foreman/test_end_to_end.py @@ -41,7 +41,8 @@ SMASHER_EXPERIMENTS = ["GSE1487313", "SRP332914"] MICROARRAY_ACCESSION_CODES = [ - "E-TABM-496", # 39 samples of SACCHAROMYCES_CEREVISIAE microarray data + # TODO(ark): enable after ArrayExpress migration is done. + # "E-TABM-496", # 39 samples of SACCHAROMYCES_CEREVISIAE microarray data "GSE94793", # 24 samples of SACCHAROMYCES_CEREVISIAE microarray data "GSE80822", # 12 samples of SACCHAROMYCES_CEREVISIAE microarray data "GSE96849", # 68 samples of SACCHAROMYCES_CEREVISIAE microarray data From 2bb7ffd68b418dddeda36d127240bf1734c258e5 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 21 Apr 2023 08:23:05 -0700 Subject: [PATCH 119/220] Bump setup-terraform action from v1 to v2 --- .github/workflows/config.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 74113e557..2f7d37042 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -12,7 +12,7 @@ jobs: steps: - uses: actions/checkout@v3 - - uses: hashicorp/setup-terraform@v1 + - uses: hashicorp/setup-terraform@v2 with: terraform_version: 0.13.5 From 007b55e89fe866224bdd46047b103ec64e80f078 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 21 Apr 2023 08:24:52 -0700 Subject: [PATCH 120/220] Bump docker login-action from v1 to v2 --- .github/workflows/config.yml | 18 +++++++++--------- 1 file changed, 9 insertions(+), 9 deletions(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 74113e557..08445aefe 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -43,7 +43,7 @@ jobs: run: ./.github/scripts/cleanup_instance.sh - name: Login to Packages Container registry - uses: docker/login-action@v1 + uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} @@ -86,7 +86,7 @@ jobs: - uses: actions/checkout@v3 - name: Login to Packages Container registry - uses: docker/login-action@v1 + uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} @@ -131,7 +131,7 @@ jobs: run: ./.github/scripts/cleanup_instance.sh - name: Login to Packages Container registry - uses: docker/login-action@v1 + uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} @@ -180,7 +180,7 @@ jobs: - uses: actions/checkout@v3 - name: Login to Packages Container registry - uses: docker/login-action@v1 + uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} @@ -232,7 +232,7 @@ jobs: run: ./.github/scripts/cleanup_instance.sh - name: Login to Packages Container registry - uses: docker/login-action@v1 + uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} @@ -281,7 +281,7 @@ jobs: - uses: actions/checkout@v3 - name: Login to Packages Container registry - uses: docker/login-action@v1 + uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} @@ -324,7 +324,7 @@ jobs: - uses: actions/checkout@v3 - name: Login to Packages Container registry - uses: docker/login-action@v1 + uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} @@ -368,7 +368,7 @@ jobs: run: ./.github/scripts/cleanup_instance.sh - name: Login to Packages Container registry - uses: docker/login-action@v1 + uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} @@ -429,7 +429,7 @@ jobs: - uses: actions/checkout@v3 - name: Login to Packages Container registry - uses: docker/login-action@v1 + uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} From 93fefb685cb34e461173bb326bab0e611fa418b1 Mon Sep 17 00:00:00 2001 From: Jaclyn Taroni <19534205+jaclyn-taroni@users.noreply.github.com> Date: Mon, 24 Apr 2023 10:23:57 -0400 Subject: [PATCH 121/220] Add newer and additional RNA-seq supported platforms --- config/supported_rnaseq_platforms.txt | 7 +++++++ 1 file changed, 7 insertions(+) diff --git a/config/supported_rnaseq_platforms.txt b/config/supported_rnaseq_platforms.txt index f4f43b61a..48754187c 100644 --- a/config/supported_rnaseq_platforms.txt +++ b/config/supported_rnaseq_platforms.txt @@ -8,8 +8,15 @@ Illumina HiSeq 2000 Illumina HiSeq 2500 Illumina HiSeq 3000 Illumina HiSeq 4000 +Illumina HiSeq X +HiSeq X Ten +HiSeq X Five +Illumina NovaSeq 6000 +Illumina NovaSeq X NextSeq 500 NextSeq 550 +NextSeq 1000 +NextSeq 2000 Ion Torrent Proton Ion Torrent S5 Ion Torrent S5 XL From 8bc04bb7193e85b6986186a270b3bf9e2d471d52 Mon Sep 17 00:00:00 2001 From: Jaclyn Taroni <19534205+jaclyn-taroni@users.noreply.github.com> Date: Wed, 26 Apr 2023 15:16:07 -0400 Subject: [PATCH 122/220] Add MiSeq --- config/supported_rnaseq_platforms.txt | 1 + 1 file changed, 1 insertion(+) diff --git a/config/supported_rnaseq_platforms.txt b/config/supported_rnaseq_platforms.txt index 48754187c..3273895c0 100644 --- a/config/supported_rnaseq_platforms.txt +++ b/config/supported_rnaseq_platforms.txt @@ -11,6 +11,7 @@ Illumina HiSeq 4000 Illumina HiSeq X HiSeq X Ten HiSeq X Five +Illumina MiSeq Illumina NovaSeq 6000 Illumina NovaSeq X NextSeq 500 From db70776c5bdf9eb82b84a35880efda140095d580 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 10 May 2023 10:57:44 -0700 Subject: [PATCH 123/220] Rework Docker image build scripts - Isolate local/staging Docker registries - Change `-d`/`-r` deployment flags meaning - Introduce prepare_image.sh `-u` flag - Introduce update_my_docker_images.sh `-b`/`-x` flags - Unify `source` operation syntax - Reformat .sh files - Standardize docker command options format --- .github/scripts/remote_deploy.sh | 6 +- .github/scripts/update_docker_img.sh | 38 ++-- README.md | 95 ++++---- api/run_tests.sh | 39 ++-- api/serve.sh | 23 +- api/serve_production.sh | 27 ++- common/dockerfiles/Dockerfile.common_tests | 3 +- common/dockerfiles/Dockerfile.migrations | 3 +- common/run_tests.sh | 38 ++-- foreman/dockerfiles/Dockerfile.foreman | 3 +- foreman/run_end_to_end_tests.sh | 28 +-- foreman/run_management_command.sh | 27 ++- foreman/run_tests.sh | 32 ++- foreman/test_survey.sh | 29 ++- .../api-server-instance-user-data.tpl.sh | 54 ++--- infrastructure/deploy.sh | 170 ++++++++------- infrastructure/destroy_terraform.sh | 17 +- .../foreman-server-instance-user-data.tpl.sh | 113 +++++----- scripts/common.sh | 26 ++- scripts/format_batch_with_env.sh | 138 ++++++------ scripts/install_all.sh | 165 +++++++------- scripts/install_db_docker.sh | 49 ++++- scripts/kill_all_jobs.sh | 7 +- scripts/make_migrations.sh | 40 ++-- scripts/prepare_image.sh | 152 +++++++------ scripts/rebuild_es_index.sh | 5 +- scripts/reinit_database.sh | 6 +- scripts/run_all_tests.sh | 5 +- scripts/run_es.sh | 18 +- scripts/run_manage.sh | 46 ++-- scripts/run_postgres.sh | 31 ++- scripts/run_psql_shell.sh | 10 +- scripts/run_shell.sh | 29 ++- scripts/update_models.sh | 15 +- scripts/update_my_docker_images.sh | 149 ++++++++----- workers/dockerfiles/Dockerfile.affymetrix | 3 +- .../dockerfiles/Dockerfile.affymetrix_local | 3 +- workers/dockerfiles/Dockerfile.downloaders | 3 +- workers/dockerfiles/Dockerfile.illumina | 3 +- workers/dockerfiles/Dockerfile.no_op | 3 +- workers/dockerfiles/Dockerfile.salmon | 3 +- workers/dockerfiles/Dockerfile.smasher | 3 +- workers/dockerfiles/Dockerfile.transcriptome | 3 +- workers/run_command.sh | 44 ++-- workers/run_janitor.sh | 37 ++-- workers/run_job.sh | 139 ++++++------ workers/run_tests.sh | 206 +++++++++--------- 47 files changed, 1185 insertions(+), 901 deletions(-) diff --git a/.github/scripts/remote_deploy.sh b/.github/scripts/remote_deploy.sh index 318aaac0c..a22618b6b 100755 --- a/.github/scripts/remote_deploy.sh +++ b/.github/scripts/remote_deploy.sh @@ -72,8 +72,8 @@ run_on_deploy_box "source env_vars && echo -e '######\nBuilding new images for $ run_on_deploy_box "source env_vars && ./.github/scripts/update_docker_img.sh 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" run_on_deploy_box "source env_vars && echo -e '######\nFinished building new images for $CI_TAG\n######' 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" -# Load docker_img_exists function and $ALL_CCDL_IMAGES -source scripts/common.sh +# Load docker_img_exists function and $ALL_IMAGES. +. scripts/common.sh if [[ "$MASTER_OR_DEV" == "master" ]]; then DOCKERHUB_REPO=ccdl @@ -89,7 +89,7 @@ fi # https://github.com/AlexsLemonade/refinebio/issues/784 # Since it's not clear how that happened, the safest thing is to add # an explicit check that the Docker images were successfully updated. -for IMAGE in $ALL_CCDL_IMAGES; do +for IMAGE in $ALL_IMAGES; do image_name="$DOCKERHUB_REPO/dr_$IMAGE" if ! docker_img_exists "$image_name" "$CI_TAG"; then echo "Docker image $image_name:$CI_TAG doesn't exist after running update_docker_img.sh!" diff --git a/.github/scripts/update_docker_img.sh b/.github/scripts/update_docker_img.sh index 620149463..3360245b3 100755 --- a/.github/scripts/update_docker_img.sh +++ b/.github/scripts/update_docker_img.sh @@ -1,8 +1,8 @@ #!/bin/bash set -e -# Load docker_img_exists function and $CCDL_WORKER_IMAGES -source ~/refinebio/scripts/common.sh +# Load docker_img_exists function and $WORKER_IMAGES +. ~/refinebio/scripts/common.sh # Github won't set the branch name for us, so do it ourselves. branch=$(get_master_or_dev "$CI_TAG") @@ -12,7 +12,7 @@ if [[ "$branch" == "master" ]]; then elif [[ "$branch" == "dev" ]]; then DOCKERHUB_REPO=ccdlstaging else - echo "Why in the world was update_docker_img.sh called from a branch other than dev or master?!?!?" + echo "Why in the world was update_docker_img.sh called from a branch other than dev or master!?" exit 1 fi @@ -20,15 +20,15 @@ echo "$CI_TAG" > ~/refinebio/common/version # Create ~/refinebio/common/dist/data-refinery-common-*.tar.gz, which is # required by the workers and data_refinery_foreman images. -## Remove old common distributions if they exist +## Remove old common distributions if they exist. rm -f ~/refinebio/common/dist/* cd ~/refinebio/common && python3 setup.py sdist -# Log into DockerHub +# Log into DockerHub. docker login -u "$DOCKER_ID" -p "$DOCKER_PASSWD" cd ~/refinebio -for IMAGE in $CCDL_WORKER_IMAGES; do +for IMAGE in $WORKER_IMAGES; do image_name="$DOCKERHUB_REPO/dr_$IMAGE" if docker_img_exists "$image_name" "$CI_TAG"; then echo "Docker image exists, skipping: $image_name:$CI_TAG" @@ -40,43 +40,45 @@ for IMAGE in $CCDL_WORKER_IMAGES; do -f "workers/dockerfiles/Dockerfile.$IMAGE" \ --build-arg SYSTEM_VERSION="$CI_TAG" . docker push "$image_name:$CI_TAG" - # Update latest version + # Update latest version. docker tag "$image_name:$CI_TAG" "$image_name:latest" docker push "$image_name:latest" - # Save some space when we're through + # Save some space when we're through. docker rmi "$image_name:$CI_TAG" fi done -# Build and push foreman image +# Build and push foreman image. FOREMAN_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_foreman" if docker_img_exists "$FOREMAN_DOCKER_IMAGE" "$CI_TAG"; then echo "Docker image exists, skipping: $FOREMAN_DOCKER_IMAGE:$CI_TAG" else # Build and push image. We use the CI_TAG as the system version. docker build \ - -t "$FOREMAN_DOCKER_IMAGE:$CI_TAG" \ - -f foreman/dockerfiles/Dockerfile.foreman \ - --build-arg SYSTEM_VERSION="$CI_TAG" . + --build-arg SYSTEM_VERSION="$CI_TAG" \ + --file foreman/dockerfiles/Dockerfile.foreman \ + --tag "$FOREMAN_DOCKER_IMAGE:$CI_TAG" \ + . docker push "$FOREMAN_DOCKER_IMAGE:$CI_TAG" - # Update latest version + # Update latest version. docker tag "$FOREMAN_DOCKER_IMAGE:$CI_TAG" "$FOREMAN_DOCKER_IMAGE:latest" docker push "$FOREMAN_DOCKER_IMAGE:latest" fi -# Build and push API image +# Build and push API image. API_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_api" if docker_img_exists "$API_DOCKER_IMAGE" "$CI_TAG"; then echo "Docker image exists, skipping: $API_DOCKER_IMAGE:$CI_TAG" else # Build and push image. We use the CI_TAG as the system version. docker build \ - -t "$API_DOCKER_IMAGE:$CI_TAG" \ - -f api/dockerfiles/Dockerfile.api_production \ - --build-arg SYSTEM_VERSION="$CI_TAG" . + --build-arg SYSTEM_VERSION="$CI_TAG" \ + --file api/dockerfiles/Dockerfile.api_production \ + --tag "$API_DOCKER_IMAGE:$CI_TAG" \ + . docker push "$API_DOCKER_IMAGE:$CI_TAG" - # Update latest version + # Update latest version. docker tag "$API_DOCKER_IMAGE:$CI_TAG" "$API_DOCKER_IMAGE:latest" docker push "$API_DOCKER_IMAGE:latest" fi diff --git a/README.md b/README.md index 35505fbfb..794a02db6 100644 --- a/README.md +++ b/README.md @@ -19,46 +19,49 @@ Refine.bio currently has four sub-projects contained within this repo: ## Table of Contents -- [Development](#development) - - [Git Workflow](#git-workflow) - - [Installation](#installation) - - [Automatic](#automatic) - - [Linux (Manual)](#linux-manual) - - [Mac (Manual)](#mac-manual) - - [Virtual Environment](#virtual-environment) - - [Services](#services) - - [Postgres](#postgres) - - [Common Dependecies](#common-dependecies) - - [ElasticSearch](#elasticsearch) - - [Testing](#testing) - - [API](#api) - - [Common](#common) - - [Foreman](#foreman) - - [Workers](#workers) - - [Style](#style) - - [Gotchas](#gotchas) - - [R](#r) -- [Running Locally](#running-locally) - - [API](#api-1) - - [Surveyor Jobs](#surveyor-jobs) - - [Sequence Read Archive](#sequence-read-archive) - - [Ensembl Transcriptome Indices](#ensembl-transcriptome-indices) - - [Downloader Jobs](#downloader-jobs) - - [Processor Jobs](#processor-jobs) - - [Creating Quantile Normalization Reference Targets](#creating-quantile-normalization-reference-targets) - - [Creating Compendia](#creating-compendia) - - [Running Tximport Early](#running-tximport-early) - - [Development Helpers](#development-helpers) -- [Cloud Deployment](#cloud-deployment) - - [Docker Images](#docker-images) - - [Terraform](#terraform) - - [Running Jobs](#running-jobs) - - [Log Consumption](#log-consumption) - - [Dumping and Restoring Database Backups](#dumping-and-restoring-database-backups) - - [Tearing Down](#tearing-down) -- [Support](#support) -- [Meta-README](#meta-readme) -- [License](#license) +- [Refine.bio ](#refinebio--) + - [Table of Contents](#table-of-contents) + - [Development](#development) + - [Git Workflow](#git-workflow) + - [Installation](#installation) + - [Automatic](#automatic) + - [Linux (Manual)](#linux-manual) + - [Mac (Manual)](#mac-manual) + - [Virtual Environment](#virtual-environment) + - [Services](#services) + - [Postgres](#postgres) + - [Common Dependecies](#common-dependecies) + - [ElasticSearch](#elasticsearch) + - [Testing](#testing) + - [API](#api) + - [Common](#common) + - [Foreman](#foreman) + - [Workers](#workers) + - [Style](#style) + - [Gotchas](#gotchas) + - [R](#r) + - [Running Locally](#running-locally) + - [API](#api-1) + - [Surveyor Jobs](#surveyor-jobs) + - [Sequence Read Archive](#sequence-read-archive) + - [Ensembl Transcriptome Indices](#ensembl-transcriptome-indices) + - [Downloader Jobs](#downloader-jobs) + - [Processor Jobs](#processor-jobs) + - [Creating Quantile Normalization Reference Targets](#creating-quantile-normalization-reference-targets) + - [Creating Compendia](#creating-compendia) + - [Running Tximport Early](#running-tximport-early) + - [Development Helpers](#development-helpers) + - [Cloud Deployment](#cloud-deployment) + - [Docker Images](#docker-images) + - [Terraform](#terraform) + - [AWS Batch](#aws-batch) + - [Running Jobs](#running-jobs) + - [Log Consumption](#log-consumption) + - [Dumping and Restoring Database Backups](#dumping-and-restoring-database-backups) + - [Tearing Down](#tearing-down) + - [Support](#support) + - [Meta-README](#meta-readme) + - [License](#license) @@ -630,17 +633,17 @@ Please try to keep the `dev` and `master` versions in sync for major and minor v Refine.bio uses a number of different Docker images to run different pieces of the system. By default, refine.bio will pull images from the Dockerhub repo `ccdlstaging`. -If you would like to use images you have built and pushed to Dockerhub yourself you can pass the `-d` option to the `deploy.sh` script. +If you would like to use images you have built and pushed to Dockerhub yourself you can pass the `-r` option to the `deploy.sh` script. To make building and pushing your own images easier, the `scripts/update_my_docker_images.sh` has been provided. -The `-d` option will allow you to specify which repo you'd like to push to. +The `-r` option will allow you to specify which repo you'd like to push to. If the Dockerhub repo requires you to be logged in, you should do so before running the script using `docker login`. The -v option allows you to specify the version, which will both end up on the Docker images you're building as the SYSTEM_VERSION environment variable and also will be the docker tag for the image. `scripts/update_my_docker_images.sh` will not build the dr_affymetrix image, because this image requires a lot of resources and time to build. -It can instead be built with `./scripts/prepare_image.sh -i affymetrix -d `. +It can instead be built with `./scripts/prepare_image.sh -i affymetrix -r `. WARNING: The affymetrix image installs a lot of data-as-R-packages and needs a lot of disk space to build the image. -It's not recommended to build the image with less than 60GB of free space on the disk that Docker runs on. +It's not recommended to build the image with less than 75GB of free space on the disk that Docker runs on. ### Terraform @@ -667,7 +670,7 @@ The correct way to deploy to the cloud is by running the `deploy.sh` script. Thi configuration steps, such as setting environment variables, setting up Batch job specifications, and performing database migrations. It can be used from the `infrastructure` directory like so: ```bash -./deploy.sh -u myusername -e dev -r us-east-1 -v v1.0.0 -d my-dockerhub-repo +./deploy.sh -u myusername -e dev -d us-east-1 -v v1.0.0 -r my-dockerhub-repo ``` This will spin up the whole system. It will usually take about 15 minutes, most of which is spent waiting for the Postgres instance to start. @@ -811,7 +814,7 @@ This can take a long time (>30 minutes)! ### Tearing Down -A stack that has been spun up via `deploy.sh -u myusername -e dev` can be taken down with `destroy_terraform.sh -u myusername -e dev -r us-east-1`. +A stack that has been spun up via `deploy.sh -u myusername -e dev` can be taken down with `destroy_terraform.sh -u myusername -e dev -d us-east-1`. The same username and environment must be passed into `destroy_terraform.sh` as were used to run `deploy.sh` either via the -e and -u options or by specifying `TF_VAR_stage` or `TF_VAR_user` so that the script knows which to take down. Note that this will prompt you for confirmation before actually destroying all of your cloud resources. diff --git a/api/run_tests.sh b/api/run_tests.sh index f81309fbd..f4353c679 100755 --- a/api/run_tests.sh +++ b/api/run_tests.sh @@ -4,27 +4,31 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. -# Ensure that postgres is running +# Ensure that Postgres is running. if ! [ "$(docker ps --filter name=drdb -q)" ]; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 exit 1 fi -# Ensure that elasticsearch is running + +# Ensure that ElasticSearch is running. if ! [ "$(docker ps --filter name=dres -q)" ]; then echo "You must start elasticsearchfirst with:" >&2 echo "./scripts/run_es.sh" >&2 exit 1 fi -project_root=$(pwd) # "cd .." called above +project_root=$(pwd) # "cd .." called above. volume_directory="$project_root/test_volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" @@ -34,18 +38,23 @@ chmod -R a+rwX "$volume_directory" ./scripts/prepare_image.sh -i api_local -s api . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) ES_HOST_IP=$(get_docker_es_ip_address) -# Only run interactively if we are on a TTY +# Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi -docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --add-host=elasticsearch:"$ES_HOST_IP" \ - --env-file api/environments/test \ - --platform linux/amd64 \ - --volume "$volume_directory":/home/user/data_store \ - ccdlstaging/dr_api_local bash -c "$(run_tests_with_coverage "$@")" +# shellcheck disable=SC2086 +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --add-host=elasticsearch:"$ES_HOST_IP" \ + --env-file api/environments/test \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + $INTERACTIVE \ + "$DOCKERHUB_REPO/dr_api_local" \ + bash -c "$(run_tests_with_coverage "$@")" diff --git a/api/serve.sh b/api/serve.sh index 4bae59fe9..1cd32a622 100755 --- a/api/serve.sh +++ b/api/serve.sh @@ -4,22 +4,29 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. ./scripts/prepare_image.sh -i api_local -s api . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) ES_HOST_IP=$(get_docker_es_ip_address) docker run \ - --add-host=database:"$DB_HOST_IP" \ - --add-host=elasticsearch:"$ES_HOST_IP" \ - --env-file api/environments/local \ - -p 8000:8000 \ - -it ccdlstaging/dr_api_local python3 manage.py runserver 0.0.0.0:8000 "$@" + --add-host=database:"$DB_HOST_IP" \ + --add-host=elasticsearch:"$ES_HOST_IP" \ + --env-file api/environments/local \ + --interactive \ + --publish 8000:8000 \ + --tty \ + "$DOCKERHUB_REPO/dr_api_local" \ + python3 manage.py runserver 0.0.0.0:8000 "$@" diff --git a/api/serve_production.sh b/api/serve_production.sh index 530b237ca..c23382778 100755 --- a/api/serve_production.sh +++ b/api/serve_production.sh @@ -4,24 +4,31 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. ./scripts/prepare_image.sh -i api_production -s api . ./scripts/common.sh -DB_HOST_IP=$(get_docker_db_ip_address) +DB_HOST_IP=$(get_docker_db_ip_address) STATIC_VOLUMES=/tmp/volumes_static docker run \ - --add-host=database:"$DB_HOST_IP" \ - --env-file api/environments/local \ - --link drdb:postgres \ - -v "$STATIC_VOLUMES":/tmp/www/static \ - -p 8081:8081 \ - -it -d ccdlstaging/dr_api_production /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" + --add-host=database:"$DB_HOST_IP" \ + --detach \ + --env-file api/environments/local \ + --interactive \ + --link drdb:postgres \ + --publish 8081:8081 \ + --tty \ + --volume "$STATIC_VOLUMES":/tmp/www/static \ + "$DOCKERHUB_REPO/dr_api_production" \ + /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" diff --git a/common/dockerfiles/Dockerfile.common_tests b/common/dockerfiles/Dockerfile.common_tests index 2a31940f4..c30ec8fdc 100644 --- a/common/dockerfiles/Dockerfile.common_tests +++ b/common/dockerfiles/Dockerfile.common_tests @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/common/dockerfiles/Dockerfile.migrations b/common/dockerfiles/Dockerfile.migrations index 00e704fa2..462989aee 100644 --- a/common/dockerfiles/Dockerfile.migrations +++ b/common/dockerfiles/Dockerfile.migrations @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/common/run_tests.sh b/common/run_tests.sh index 51c564270..41eb8e3d1 100755 --- a/common/run_tests.sh +++ b/common/run_tests.sh @@ -1,24 +1,27 @@ #!/bin/sh -e -# script for executing Django PyUnit Tests within a Docker container. +# Script for executing Django PyUnit tests within a Docker container. # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. -# Ensure that postgres is running +# Ensure that Postgres is running. if ! [ "$(docker ps --filter name=drdb -q)" ]; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 exit 1 fi -project_root=$(pwd) # "cd .." called above +project_root=$(pwd) # "cd .." called above. volume_directory="$project_root/test_volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" @@ -28,18 +31,23 @@ chmod -R a+rwX "$volume_directory" ./scripts/prepare_image.sh -i common_tests -s common . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) ES_HOST_IP=$(get_docker_es_ip_address) -# Only run interactively if we are on a TTY +# Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi -docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --add-host=elasticsearch:"$ES_HOST_IP" \ - --env-file common/environments/test \ - --platform linux/amd64 \ - --volume "$volume_directory":/home/user/data_store \ - ccdlstaging/dr_common_tests bash -c "$(run_tests_with_coverage "$@")" --parallel +# shellcheck disable=SC2086 +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --add-host=elasticsearch:"$ES_HOST_IP" \ + --env-file common/environments/test \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + $INTERACTIVE \ + "$DOCKERHUB_REPO/dr_common_tests" \ + bash -c "$(run_tests_with_coverage "$@")" --parallel diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 94e5c185a..2f749e4fd 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/foreman/run_end_to_end_tests.sh b/foreman/run_end_to_end_tests.sh index 554068980..e08112ae2 100755 --- a/foreman/run_end_to_end_tests.sh +++ b/foreman/run_end_to_end_tests.sh @@ -4,7 +4,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Set up the data volume directory if it does not already exist. @@ -25,30 +28,27 @@ if [ ! -e "$reference_file_dir/$quant_file" ]; then mkdir -p "$reference_file_dir" echo "Downloading quant file for Transcriptome Index validation tests." wget -q -O "$reference_file_dir/$quant_file" \ - "$test_data_repo/$quant_file" + "$test_data_repo/$quant_file" fi # temp for testing locally. ../scripts/prepare_image.sh -i foreman -s foreman - while read -r row; do # Exporting an expansion rather than a variable, which is exactly what we want to do. # shellcheck disable=SC2163 export "${row}" -done < ../infrastructure/prod_env - +done <../infrastructure/prod_env -# Hardcode ccdlstaging because this should only ever be run in staging or -# locally, and when running locally `prepare_image.sh` makes the forman -# ccdlstaging/dr_foreman. -docker run -t \ - --env-file ../infrastructure/prod_env \ - --env RUNNING_IN_CLOUD=False \ +docker run \ --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ + --env DJANGO_SECRET_KEY="TEST_KEY_FOR_DEV" \ --env JOB_DEFINITION_PREFIX="$USER_$STAGE_" \ --env REFINEBIO_BASE_URL="http://$API_HOST/v1/" \ - --env DJANGO_SECRET_KEY="TEST_KEY_FOR_DEV" \ - --volume "$volume_directory":/home/user/data_store \ + --env RUNNING_IN_CLOUD=False \ + --env-file ../infrastructure/prod_env \ --volume "$HOME/.aws":/home/user/.aws \ - ccdlstaging/dr_foreman python3 manage.py test --no-input --parallel=2 --testrunner='tests.test_runner.NoDbTestRunner' tests.foreman.test_end_to_end + --volume "$volume_directory":/home/user/data_store \ + --tty \ + "$DOCKERHUB_REPO/dr_foreman" \ + python3 manage.py test --no-input --parallel=2 --testrunner='tests.test_runner.NoDbTestRunner' tests.foreman.test_end_to_end diff --git a/foreman/run_management_command.sh b/foreman/run_management_command.sh index a9e4ce101..d51768fd8 100755 --- a/foreman/run_management_command.sh +++ b/foreman/run_management_command.sh @@ -1,13 +1,16 @@ #!/bin/sh -# Script for running the Data Refinery Surveyor container +# Script for running the Data Refinery Surveyor container. # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to +# However, in order to give Docker access to all the code we have to # move up a level cd .. @@ -21,12 +24,16 @@ chmod -R a+rwX "$volume_directory" ./scripts/prepare_image.sh -i foreman -s foreman . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) -docker run -it \ - --add-host=database:"$DB_HOST_IP" \ - --env-file foreman/environments/local \ - --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ - --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ - --volume "$volume_directory":/home/user/data_store \ - ccdlstaging/dr_foreman python3 manage.py "$@" +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ + --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ + --env-file foreman/environments/local \ + --interactive \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + "$DOCKERHUB_REPO/dr_foreman" \ + python3 manage.py "$@" diff --git a/foreman/run_tests.sh b/foreman/run_tests.sh index 8edbdc56f..f0ae37de4 100755 --- a/foreman/run_tests.sh +++ b/foreman/run_tests.sh @@ -4,7 +4,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Set up the data volume directory if it does not already exist. @@ -18,11 +21,11 @@ if [ ! -d "$volume_directory" ]; then fi chmod -R a+rwX "$volume_directory" -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. -# First ensure postgres is running +# First ensure Postgres is running. if ! [ "$(docker ps --filter name=drdb -q)" ]; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 @@ -32,16 +35,21 @@ fi ./scripts/prepare_image.sh -i foreman -s foreman . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) -# Only run interactively if we are on a TTY +# Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi -docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --env-file foreman/environments/test \ - --platform linux/amd64 \ - --volume "$volume_directory":/home/user/data_store \ - ccdlstaging/dr_foreman bash -c "$(run_tests_with_coverage --exclude-tag=manual "$@")" +# shellcheck disable=SC2086 +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --env-file foreman/environments/test \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + $INTERACTIVE \ + "$DOCKERHUB_REPO/dr_foreman" \ + bash -c "$(run_tests_with_coverage --exclude-tag=manual "$@")" diff --git a/foreman/test_survey.sh b/foreman/test_survey.sh index 7df91a3ae..1aec44116 100755 --- a/foreman/test_survey.sh +++ b/foreman/test_survey.sh @@ -2,7 +2,7 @@ # Script for testing the surveying of an accession manually (e.g. from a dataset request) print_options() { - cat << EOF + cat <&2 echo "./scripts/run_postgres.sh" >&2 @@ -76,12 +79,16 @@ fi ./scripts/prepare_image.sh -i foreman -s foreman . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) docker run \ - --add-host=database:"$DB_HOST_IP" \ - --env-file foreman/environments/test \ - --volume "$volume_directory":/home/user/data_store \ - -e SURVEYOR="$SURVEYOR" \ - -e ACCESSION="$ACCESSION" \ - -it ccdlstaging/dr_foreman bash -c "python3 manage.py test --tag=manual ." + --add-host=database:"$DB_HOST_IP" \ + --env ACCESSION="$ACCESSION" \ + --env SURVEYOR="$SURVEYOR" \ + --env-file foreman/environments/test \ + --interactive \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + "$DOCKERHUB_REPO/dr_foreman" \ + bash -c "python3 manage.py test --tag=manual ." diff --git a/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh b/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh index 3f475c98b..0dfb15ddc 100644 --- a/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh +++ b/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh @@ -15,7 +15,7 @@ cd /home/ubuntu || exit # Install and configure Nginx. -cat <<"EOF" > nginx.conf +cat <<"EOF" >nginx.conf ${nginx_config} EOF apt-get update -y @@ -107,7 +107,7 @@ echo " size 20k daily maxage 3 -}" >> /etc/logrotate.conf +}" >>/etc/logrotate.conf echo " /tmp/access.log { missingok @@ -116,10 +116,10 @@ echo " size 20k daily maxage 3 -}" >> /etc/logrotate.conf +}" >>/etc/logrotate.conf # Install our environment variables -cat <<"EOF" > environment +cat <<"EOF" >environment ${api_environment} EOF @@ -135,36 +135,40 @@ docker pull "${dockerhub_repo}/${api_docker_image}" # These database values are created after TF # is run, so we have to pass them in programatically docker run \ - --env-file environment \ - -e DATABASE_HOST="${database_host}" \ - -e DATABASE_NAME="${database_name}" \ - -e DATABASE_USER="${database_user}" \ - -e DATABASE_PASSWORD="${database_password}" \ - -e ELASTICSEARCH_HOST="${elasticsearch_host}" \ - -e ELASTICSEARCH_PORT="${elasticsearch_port}" \ - -v "$STATIC_VOLUMES":/tmp/www/static \ - --log-driver=awslogs \ - --log-opt awslogs-region="${region}" \ - --log-opt awslogs-group="${log_group}" \ - --log-opt awslogs-stream="${log_stream}" \ - -p 8081:8081 \ - --name=dr_api \ - -it -d "${dockerhub_repo}/${api_docker_image}" /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" + --detach \ + --env DATABASE_HOST="${database_host}" \ + --env DATABASE_NAME="${database_name}" \ + --env DATABASE_PASSWORD="${database_password}" \ + --env DATABASE_USER="${database_user}" \ + --env ELASTICSEARCH_HOST="${elasticsearch_host}" \ + --env ELASTICSEARCH_PORT="${elasticsearch_port}" \ + --env-file environment \ + --interactive \ + --log-driver=awslogs \ + --log-opt awslogs-group="${log_group}" \ + --log-opt awslogs-region="${region}" \ + --log-opt awslogs-stream="${log_stream}" \ + --name=dr_api \ + --tty \ + --volume "$STATIC_VOLUMES":/tmp/www/static \ + -publish 8081:8081 \ + "${dockerhub_repo}/${api_docker_image}" \ + /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" # Nuke and rebuild the search index. It shouldn't take too long. sleep 30 -docker exec dr_api python3 manage.py search_index --delete -f; -docker exec dr_api python3 manage.py search_index --rebuild -f; -docker exec dr_api python3 manage.py search_index --populate -f; +docker exec dr_api python3 manage.py search_index --delete -f +docker exec dr_api python3 manage.py search_index --rebuild -f +docker exec dr_api python3 manage.py search_index --populate -f # Let's use this instance to call the populate command every twenty minutes. -crontab -l > tempcron +crontab -l >tempcron # echo new cron into cron file # TODO: stop logging this to api_cron.log once we figure out why it # hasn't been working. -echo -e "SHELL=/bin/bash\nPATH=/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin\n*/20 * * * * docker exec dr_api python3 manage.py update_es_index >> /var/log/api_cron.log 2>&1" >> tempcron +echo -e "SHELL=/bin/bash\nPATH=/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin\n*/20 * * * * docker exec dr_api python3 manage.py update_es_index >> /var/log/api_cron.log 2>&1" >>tempcron # Post a summary of downloads every Monday at 12:00 UTC -echo -e "SHELL=/bin/bash\nPATH=/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin\n0 12 * * MON docker exec dr_api python3 manage.py post_downloads_summary >> /var/log/api_cron.log 2>&1" >> tempcron +echo -e "SHELL=/bin/bash\nPATH=/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin\n0 12 * * MON docker exec dr_api python3 manage.py post_downloads_summary >> /var/log/api_cron.log 2>&1" >>tempcron # install new cron file crontab tempcron rm tempcron diff --git a/infrastructure/deploy.sh b/infrastructure/deploy.sh index d8175b423..2298c2d8a 100755 --- a/infrastructure/deploy.sh +++ b/infrastructure/deploy.sh @@ -2,7 +2,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit print_description() { @@ -21,7 +24,7 @@ print_options() { echo ' "-e prod" will deploy the production stack. This should only be used from a CD machine.' echo ' "-e staging" will deploy the staging stack. This should only be used from a CD machine.' echo ' "-e dev" will deploy a dev stack which is appropriate for a single developer to use to test.' - echo '-d May be used to override the Dockerhub repo where the images will be pulled from.' + echo '-r May be used to override the Dockerhub repo where the images will be pulled from.' echo ' This may also be specified by setting the TF_VAR_dockerhub_repo environment variable.' echo ' If unset, defaults to "ccdlstaging" if the version contains "-dev" and "ccdl" otherwise.' echo ' for dev and staging environments and "ccdl" for prod.' @@ -31,7 +34,7 @@ print_options() { echo '-v specifies the version of the system which is being deployed and is not optional.' echo "-u specifies the username of the deployer. Should be the developer's name in development stacks." echo ' This option may be omitted, in which case the TF_VAR_user variable MUST be set instead.' - echo '-r specifies the AWS region to deploy the stack to. Defaults to us-east-1.' + echo '-d specifies the AWS region to deploy the stack to. Defaults to us-east-1.' } while getopts ":e:d:i:v:u:r:h" opt; do @@ -40,7 +43,7 @@ while getopts ":e:d:i:v:u:r:h" opt; do export env=$OPTARG export TF_VAR_stage=$OPTARG ;; - d) + r) export TF_VAR_dockerhub_repo=$OPTARG ;; i) @@ -53,7 +56,7 @@ while getopts ":e:d:i:v:u:r:h" opt; do u) export TF_VAR_user=$OPTARG ;; - r) + d) export TF_VAR_region=$OPTARG ;; h) @@ -102,31 +105,29 @@ if [[ -z $TF_VAR_region ]]; then TF_VAR_region=us-east-1 fi - # We have terraform output environment variables via a single output # variable, which we then read in as json using the command line tool # `jq`, so that we can use them via bash. -format_environment_variables () { - json_env_vars=$(terraform output -json environment_variables | jq -c '.[]') - for row in $json_env_vars; do - name=$(echo "$row" | jq -r ".name") - value=$(echo "$row" | jq -r ".value") - env_var_assignment="$name=$value" - # Exporting an expansion rather than a variable, which is exactly what we want to do. - # shellcheck disable=SC2163 - export "${env_var_assignment?}" - echo "$env_var_assignment" >> prod_env - done +format_environment_variables() { + json_env_vars=$(terraform output -json environment_variables | jq -c '.[]') + for row in $json_env_vars; do + name=$(echo "$row" | jq -r ".name") + value=$(echo "$row" | jq -r ".value") + env_var_assignment="$name=$value" + # Exporting an expansion rather than a variable, which is exactly what we want to do. + # shellcheck disable=SC2163 + export "${env_var_assignment?}" + echo "$env_var_assignment" >>prod_env + done } - -# Load $ALL_CCDL_IMAGES and helper functions -source ../scripts/common.sh +# Load $ALL_IMAGES and helper functions. +. ../scripts/common.sh # Make our IP address known to terraform. TF_VAR_host_ip="$(dig +short myip.opendns.com @resolver1.opendns.com)" export TF_VAR_host_ip -for IMAGE in $ALL_CCDL_IMAGES; do +for IMAGE in $ALL_IMAGES; do # For each image we need to set the env var that is used by our # scripts and the env var that gets picked up by terraform because # it is preceeded with TF_VAR. @@ -140,27 +141,26 @@ cp deploy/ci_ingress.tf . # Check if a new ccdl-ubuntu ami will be needed for this region if [[ $(aws ec2 describe-images \ - --region $TF_VAR_region --owners 589864003899 \ - --filters 'Name=name,Values=ccdl-ubuntu-18.04-*' \ - --query 'length(Images)') \ - -eq 0 ]]; then + --region "$TF_VAR_region" --owners 589864003899 \ + --filters 'Name=name,Values=ccdl-ubuntu-18.04-*' \ + --query 'length(Images)') -eq 0 ]]; then echo "No ccdl-ubuntu-18.04 AMI found for this region, creating a new one" # Find most recent ccdl-ubuntu ami from us-east-1 template_ami_id=$(aws ec2 describe-images \ - --region us-east-1 --owners 589864003899 \ - --filters 'Name=name,Values=ccdl-ubuntu-18.04-*' \ - --query 'sort_by(Images,&CreationDate)[-1].ImageId' \ - --output text) + --region us-east-1 --owners 589864003899 \ + --filters 'Name=name,Values=ccdl-ubuntu-18.04-*' \ + --query 'sort_by(Images,&CreationDate)[-1].ImageId' \ + --output text) # Make a copy into this region new_ami_name="ccdl-ubuntu-18.04-$(date "+%Y-%m-%dT%H.%M.%S")" new_ami_id=$(aws ec2 copy-image \ - --source-image-id "$template_ami_id" \ - --source-region us-east-1 \ - --region "$TF_VAR_region" \ - --name "$new_ami_name" \ - --output text) + --source-image-id "$template_ami_id" \ + --source-region us-east-1 \ + --region "$TF_VAR_region" \ + --name "$new_ami_name" \ + --output text) echo "Created new AMI for $TF_VAR_region" echo " name: $new_ami_name" echo " id: $new_ami_id" @@ -174,16 +174,16 @@ fi terraform taint module.batch.aws_launch_template.data_refinery_worker || true terraform taint module.batch.aws_launch_template.data_refinery_compendia || true if terraform state list | grep -q module.batch.aws_batch_job_queue.data_refinery_; then - terraform state list \ - | grep module.batch.aws_batch_job_queue.data_refinery_ \ - | xargs -L 1 terraform taint \ - || true + terraform state list | + grep module.batch.aws_batch_job_queue.data_refinery_ | + xargs -L 1 terraform taint || + true fi if terraform state list | grep -q module.batch.aws_batch_compute_environment.data_refinery__; then - terraform state list \ - | grep module.batch.aws_batch_compute_environment.data_refinery_ \ - | xargs -L 1 terraform taint \ - || true + terraform state list | + grep module.batch.aws_batch_compute_environment.data_refinery_ | + xargs -L 1 terraform taint || + true fi if terraform output | grep -q 'No outputs found'; then @@ -232,7 +232,7 @@ format_environment_variables # Make sure to clear out any old batch job templates since we # will register everything in this directory. if [ -e batch-job-templates ]; then - rm -r batch-job-templates + rm -r batch-job-templates fi # Template the environment variables for production into the Batch Job @@ -272,47 +272,49 @@ docker pull "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" # Test that the pg_bouncer instance is up. 15 minutes should be more than enough. start_time=$(date +%s) diff=0 -until pg_isready -d "$DATABASE_NAME" -h "$DATABASE_PUBLIC_HOST" -p "$DATABASE_PORT" -U "$DATABASE_USER" &> /dev/null || [ "$diff" -gt "900" ] -do +until pg_isready -d "$DATABASE_NAME" -h "$DATABASE_PUBLIC_HOST" -p "$DATABASE_PORT" -U "$DATABASE_USER" &>/dev/null || [ "$diff" -gt "900" ]; do echo "Waiting for the pg_bouncer instance to come online ..." sleep 10 - (( diff = $(date +%s) - start_time )) + ((diff = $(date +%s) - start_time)) done -if ! pg_isready -d "$DATABASE_NAME" -h "$DATABASE_PUBLIC_HOST" -p "$DATABASE_PORT" -U "$DATABASE_USER" &> /dev/null; then +if ! pg_isready -d "$DATABASE_NAME" -h "$DATABASE_PUBLIC_HOST" -p "$DATABASE_PORT" -U "$DATABASE_USER" &>/dev/null; then echo "pg_bouncer instance failed to come up after 15 minutes." exit 1 fi # Migrate auth. docker run \ - --env-file prod_env \ - --env RUNNING_IN_CLOUD=False \ - --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ - "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" python3 manage.py migrate auth + --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ + --env RUNNING_IN_CLOUD=False \ + --env-file prod_env \ + "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ + python3 manage.py migrate auth # Apply general migrations. docker run \ - --env-file prod_env \ - --env RUNNING_IN_CLOUD=False \ - --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ - "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" python3 manage.py migrate + --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ + --env RUNNING_IN_CLOUD=False \ + --env-file prod_env \ + "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ + python3 manage.py migrate # Create the cache table if it does not already exist. docker run \ - --env-file prod_env \ - --env RUNNING_IN_CLOUD=False \ - --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ - "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" python3 manage.py createcachetable + --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ + --env RUNNING_IN_CLOUD=False \ + --env-file prod_env \ + "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ + python3 manage.py createcachetable # Terraform doesn't manage these well, so they need to be tainted to # ensure they won't require manual intervention. terraform taint module.batch.aws_launch_template.data_refinery_worker terraform taint module.batch.aws_launch_template.data_refinery_compendia -terraform state list \ - | grep module.batch.aws_batch_job_queue.data_refinery_ \ - | xargs -L 1 terraform taint \ - || true +terraform state list | + grep module.batch.aws_batch_job_queue.data_refinery_ | + xargs -L 1 terraform taint || + true # Ensure the latest image version is being used for the Foreman terraform taint aws_instance.foreman_server_1 @@ -339,10 +341,10 @@ API_IP_ADDRESS=$(terraform output -json api_server_1_ip | tr -d '"') # it's not found then grep will return a non-zero exit code so in that # case return an empty string. container_running=$(ssh -o StrictHostKeyChecking=no \ - -o ServerAliveInterval=15 \ - -o ConnectTimeout=5 \ - -i data-refinery-key.pem \ - "ubuntu@$API_IP_ADDRESS" "docker ps -a" 2> /dev/null | grep dr_api || echo "") + -o ServerAliveInterval=15 \ + -o ConnectTimeout=5 \ + -i data-refinery-key.pem \ + "ubuntu@$API_IP_ADDRESS" "docker ps -a" 2>/dev/null | grep dr_api || echo "") # If $container_running is empty, then it's because the container isn't running. # If the container isn't running, then it's because the instance is spinning up. @@ -377,21 +379,25 @@ if [[ -n $container_running ]]; then -o ConnectTimeout=5 \ -i data-refinery-key.pem \ "ubuntu@$API_IP_ADDRESS" "docker run \ - --env-file environment \ - -e DATABASE_HOST=$DATABASE_HOST \ - -e DATABASE_NAME=$DATABASE_NAME \ - -e DATABASE_USER=$DATABASE_USER \ - -e DATABASE_PASSWORD=$DATABASE_PASSWORD \ - -e ELASTICSEARCH_HOST=$ELASTICSEARCH_HOST \ - -e ELASTICSEARCH_PORT=$ELASTICSEARCH_PORT \ - -v /tmp/volumes_static:/tmp/www/static \ - --log-driver=awslogs \ - --log-opt awslogs-region=$AWS_REGION \ - --log-opt awslogs-group=data-refinery-log-group-$USER-$STAGE \ - --log-opt awslogs-stream=log-stream-api-$USER-$STAGE \ - -p 8081:8081 \ - --name=dr_api \ - -it -d $DOCKERHUB_REPO/$API_DOCKER_IMAGE /bin/sh -c /home/user/collect_and_run_uwsgi.sh" + --detach \ + --env DATABASE_HOST=$DATABASE_HOST \ + --env DATABASE_NAME=$DATABASE_NAME \ + --env DATABASE_PASSWORD=$DATABASE_PASSWORD \ + --env DATABASE_USER=$DATABASE_USER \ + --env ELASTICSEARCH_HOST=$ELASTICSEARCH_HOST \ + --env ELASTICSEARCH_PORT=$ELASTICSEARCH_PORT \ + --env-file environment \ + --interactive \ + --log-driver=awslogs \ + --log-opt awslogs-group=data-refinery-log-group-$USER-$STAGE \ + --log-opt awslogs-region=$AWS_REGION \ + --log-opt awslogs-stream=log-stream-api-$USER-$STAGE \ + --name=dr_api \ + --tty \ + --volume /tmp/volumes_static:/tmp/www/static \ + --publish 8081:8081 \ + $DOCKERHUB_REPO/$API_DOCKER_IMAGE \ + /bin/sh -c /home/user/collect_and_run_uwsgi.sh" # Don't leave secrets lying around. ssh -o StrictHostKeyChecking=no \ diff --git a/infrastructure/destroy_terraform.sh b/infrastructure/destroy_terraform.sh index cf91053e4..4329cf885 100755 --- a/infrastructure/destroy_terraform.sh +++ b/infrastructure/destroy_terraform.sh @@ -2,7 +2,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit print_description() { @@ -10,17 +13,17 @@ print_description() { } print_options() { - echo 'This script accepts the following arguments: -e, -u, -r, and -h.' - echo 'Neither -e, -u or -r is optional unless TF_VAR_stage, TF_VAR_user,' + echo 'This script accepts the following arguments: -e, -u, -d, and -h.' + echo 'Neither -e, -u or -d is optional unless TF_VAR_stage, TF_VAR_user,' echo 'or TF_VAR_region is set, respectively.' echo '-h prints this help message and exits.' echo '-e specifies the environment you would like to destroy.' echo '-u specifies the username you used to spin up the stack.' - echo '-r specifies the region of the stack to destroy.' + echo '-d specifies the region of the stack to destroy.' echo 'All arguments are needed to determine which stack to destroy.' } -while getopts ":e:u:r:h" opt; do +while getopts ":e:u:d:h" opt; do case $opt in e) export env=$OPTARG @@ -29,7 +32,7 @@ while getopts ":e:u:r:h" opt; do u) export TF_VAR_user=$OPTARG ;; - r) + d) export TF_VAR_region=$OPTARG ;; h) @@ -62,7 +65,7 @@ if [[ -z $TF_VAR_user ]]; then fi if [[ -z $TF_VAR_region ]]; then - echo 'Error: must specify region by either providing the -r argument or setting TF_VAR_region.' + echo 'Error: must specify region by either providing the -d argument or setting TF_VAR_region.' exit 1 fi diff --git a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh index c53d368bf..efc292163 100644 --- a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh +++ b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh @@ -15,30 +15,34 @@ cd /home/ubuntu || exit # Install our environment variables -cat <<"EOF" > environment +cat <<"EOF" >environment ${foreman_environment} EOF # These database values are created after TF # is run, so we have to pass them in programatically -cat >> /home/ubuntu/run_foreman.sh <>/home/ubuntu/run_foreman.sh <> /home/ubuntu/run_management_command.sh + --detach \\ + --env DATABASE_HOST=${database_host} \\ + --env DATABASE_NAME=${database_name} \\ + --env DATABASE_PASSWORD=${database_password} \\ + --env DATABASE_USER=${database_user} \\ + --env-file /home/ubuntu/environment \\ + --interactive \\ + --tty \\ + --volume /tmp:/tmp \\ + ${dockerhub_repo}/${foreman_docker_image} \\ + python3 manage.py \"\$@\" +" >>/home/ubuntu/run_management_command.sh chmod +x /home/ubuntu/run_management_command.sh echo " @@ -70,21 +78,24 @@ echo " # the first argument followed by the management command to run. docker run \\ - --env-file /home/ubuntu/environment \\ - -e DATABASE_HOST=${database_host} \\ - -e DATABASE_NAME=${database_name} \\ - -e DATABASE_USER=${database_user} \\ - -e DATABASE_PASSWORD=${database_password} \\ - -v /tmp:/tmp \\ - -it ${dockerhub_repo}/dr_\"\$1\" python3 manage.py \"\$2\" -" >> /home/ubuntu/run_manage_command.sh + --env DATABASE_HOST=${database_host} \\ + --env DATABASE_NAME=${database_name} \\ + --env DATABASE_PASSWORD=${database_password} \\ + --env DATABASE_USER=${database_user} \\ + --env-file /home/ubuntu/environment \\ + --interactive \\ + --tty \\ + --volume /tmp:/tmp \\ + ${dockerhub_repo}/dr_\"\$1\" \\ + python3 manage.py \"\$2\" +" >>/home/ubuntu/run_manage_command.sh chmod +x /home/ubuntu/run_manage_command.sh # Use Monit to ensure the Foreman is always running apt-get -y update apt-get -y install monit htop -date +%s > /tmp/foreman_last_time +date +%s >/tmp/foreman_last_time chown ubuntu:ubuntu /tmp/foreman_last_time # shellcheck disable=2016 echo ' @@ -96,7 +107,7 @@ if (( $difftime > 1800 )); then exit 1; fi exit 0; -' >> /home/ubuntu/foreman_status.sh +' >>/home/ubuntu/foreman_status.sh chmod +x /home/ubuntu/foreman_status.sh echo ' @@ -105,13 +116,13 @@ check program foreman with path "/bin/bash /home/ubuntu/foreman_status.sh" as ui if status != 0 then restart set daemon 900 -' >> /etc/monit/monitrc +' >>/etc/monit/monitrc service monit restart # Install the cron job tests -crontab -l > tempcron -cat <> tempcron +crontab -l >tempcron +cat <>tempcron 0 12 * * MON /bin/bash /home/ubuntu/run_manage_command.sh affymetrix check_brainarray_gene_agreement >> /var/log/affymetrix_checks.log 2>&1 0 12 * * MON /bin/bash /home/ubuntu/run_manage_command.sh affymetrix check_tx_index_transcript_agreement >> /var/log/affymetrix_checks.log 2>&1 0 12 * * ${accession_gathering_job_run_day} /bin/bash /home/ubuntu/run_manage_command.sh foreman gather_weekly_accessions >> /var/log/weekly_accessions.log 2>&1 @@ -122,18 +133,22 @@ rm tempcron # Make sure every downloader job has a processor job! docker run \ - --env-file /home/ubuntu/environment \ - -e DATABASE_HOST="${database_host}" \ - -e DATABASE_NAME="${database_name}" \ - -e DATABASE_USER="${database_user}" \ - -e DATABASE_PASSWORD="${database_password}" \ - -v /tmp:/tmp \ - --log-driver=awslogs \ - --log-opt awslogs-region="${region}" \ - --log-opt awslogs-group="${log_group}" \ - --log-opt awslogs-stream="log-stream-foreman-${user}-${stage}" \ - --name=job_filler \ - -it -d "${dockerhub_repo}/${foreman_docker_image}" python3 manage.py create_missing_processor_jobs + --detach \ + --env DATABASE_HOST="${database_host}" \ + --env DATABASE_NAME="${database_name}" \ + --env DATABASE_PASSWORD="${database_password}" \ + --env DATABASE_USER="${database_user}" \ + --env-file /home/ubuntu/environment \ + --interactive \ + --log-driver=awslogs \ + --log-opt awslogs-group="${log_group}" \ + --log-opt awslogs-region="${region}" \ + --log-opt awslogs-stream="log-stream-foreman-${user}-${stage}" \ + --name=job_filler \ + --tty \ + --volume /tmp:/tmp \ + "${dockerhub_repo}/${foreman_docker_image}" \ + python3 manage.py create_missing_processor_jobs # Delete the cloudinit and syslog in production. export STAGE=${stage} diff --git a/scripts/common.sh b/scripts/common.sh index 612de1a7b..07191400e 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -2,16 +2,18 @@ # These are lists of docker images that we use. The actual names end # up being /dr_ but this is useful for scripting. -export ALL_CCDL_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders foreman api" +export ALL_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders foreman api" # Sometimes we only want to work with the worker images. -export CCDL_WORKER_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders" +export WORKER_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders" +# Default Docker registry. +export DOCKERHUB_REPO="ccdlstaging" -get_docker_db_ip_address () { - docker inspect -f '{{range .NetworkSettings.Networks}}{{.IPAddress}}{{end}}' drdb 2> /dev/null +get_docker_db_ip_address() { + docker inspect -f '{{range .NetworkSettings.Networks}}{{.IPAddress}}{{end}}' drdb 2>/dev/null } -get_docker_es_ip_address () { - docker inspect -f '{{range .NetworkSettings.Networks}}{{.IPAddress}}{{end}}' dres 2> /dev/null +get_docker_es_ip_address() { + docker inspect -f '{{range .NetworkSettings.Networks}}{{.IPAddress}}{{end}}' dres 2>/dev/null } # `coverage report -m` will always have an exit code of 0 which makes @@ -19,7 +21,7 @@ get_docker_es_ip_address () { # of running the tests as $exit_code, then report the coverage, and # then exit with the appropriate code. # This is done a function so arguments to the tests can be passed through. -run_tests_with_coverage () { +run_tests_with_coverage() { COVERAGE="coverage run --source=\".\" manage.py test --settings=tests.settings --no-input $*; exit_code=\$?;" SAVE_REPORT="coverage xml -o data_store/coverage.xml;" PRINT_REPORT="coverage report -m;" @@ -33,11 +35,11 @@ run_tests_with_coverage () { # https://stackoverflow.com/questions/32113330/check-if-imagetag-combination-already-exists-on-docker-hub docker_img_exists() { TOKEN=$(curl -s -H "Content-Type: application/json" -X POST \ - -d '{"username": "'"${DOCKER_ID}"'", "password": "'"${DOCKER_PASSWD}"'"}' \ - https://hub.docker.com/v2/users/login/ | jq -r .token) + -d '{"username": "'"${DOCKER_ID}"'", "password": "'"${DOCKER_PASSWD}"'"}' \ + https://hub.docker.com/v2/users/login/ | jq -r .token) EXISTS=$(curl -s -H "Authorization: JWT ${TOKEN}" \ - "https://hub.docker.com/v2/repositories/$1/tags/?page_size=10000" \ - | jq -r "[.results | .[] | .name == \"$2\"] | any" 2> /dev/null) + "https://hub.docker.com/v2/repositories/$1/tags/?page_size=10000" | + jq -r "[.results | .[] | .name == \"$2\"] | any" 2>/dev/null) test -n "$EXISTS" -a "$EXISTS" = true } @@ -57,7 +59,7 @@ get_master_or_dev() { # All dev versions should end with '-dev' or '-dev-hotfix' and all master versions should not. if [ -n "$master_check" ] && ! echo "$version" | grep -Eq "\-dev(\-hotfix)?$"; then echo "master" - elif [ -n "$dev_check" ] ; then + elif [ -n "$dev_check" ]; then echo "dev" else echo "unknown" diff --git a/scripts/format_batch_with_env.sh b/scripts/format_batch_with_env.sh index a86ee36b5..21938e1e5 100755 --- a/scripts/format_batch_with_env.sh +++ b/scripts/format_batch_with_env.sh @@ -24,34 +24,34 @@ print_options() { while getopts ":p:e:o:v:h" opt; do case $opt in - p) - export project="$OPTARG" - ;; - e) - export env="$OPTARG" - ;; - o) - export output_dir="$OPTARG" - ;; - v) - export system_version="$OPTARG" - ;; - h) - print_description - echo - print_options - exit 0 - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - print_options >&2 - exit 1 - ;; - :) - echo "Option -$OPTARG requires an argument." >&2 - print_options >&2 - exit 1 - ;; + p) + export project="$OPTARG" + ;; + e) + export env="$OPTARG" + ;; + o) + export output_dir="$OPTARG" + ;; + v) + export system_version="$OPTARG" + ;; + h) + print_description + echo + print_options + exit 0 + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + print_options >&2 + exit 1 + ;; + :) + echo "Option -$OPTARG requires an argument." >&2 + print_options >&2 + exit 1 + ;; esac done @@ -102,10 +102,12 @@ if [ -z "$COMPENDIA_DOCKER_IMAGE" ]; then export COMPENDIA_DOCKER_IMAGE="dr_compendia:$system_version" fi - # This script should always run from the context of the directory of # the project it is building. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" project_directory="$script_directory/.." @@ -142,7 +144,7 @@ while read -r line; do # shellcheck disable=SC2163 export "$line" fi -done < "$environment_file" +done <"$environment_file" # If output_dir wasn't specified then assume the same folder we're # getting the templates from. @@ -154,7 +156,6 @@ if [ ! -d "$output_dir" ]; then mkdir "$output_dir" fi - # Not quite sure how to deal with this just yet, so punt. # export INDEX=0 @@ -163,7 +164,7 @@ fi if [ "$project" = "workers" ]; then # Iterate over all the template files in the directory. for template in batch-job-templates/*.tpl.json; do - template="$(basename "$template")" + template="$(basename "$template")" # Strip off the trailing .tpl for once we've formatted it. OUTPUT_BASE="$(basename "$template" .tpl.json)" FILETYPE=".json" @@ -173,66 +174,62 @@ if [ "$project" = "workers" ]; then if [ "$OUTPUT_FILE" = "downloader.json" ]; then rams="1024 4096 16384" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/downloader$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done echo "Made $output_dir/$OUTPUT_FILE" elif [ "$OUTPUT_FILE" = "create_compendia.json" ]; then rams="30000 950000" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/$OUTPUT_BASE$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done echo "Made $output_dir/$OUTPUT_FILE" elif [ "$OUTPUT_FILE" = "create_quantpendia.json" ]; then rams="30000 131000" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/$OUTPUT_BASE$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done echo "Made $output_dir/$OUTPUT_FILE" # From https://unix.stackexchange.com/a/111517 elif (echo "$NO_RAM_JOB_FILES" | grep -Fqw "$OUTPUT_FILE"); then perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$output_dir/$OUTPUT_FILE" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$output_dir/$OUTPUT_FILE" \ + 2>/dev/null echo "Made $output_dir/$OUTPUT_FILE" else rams="2048 4096 8192 12288 16384 32768 65536" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/$OUTPUT_BASE$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done fi @@ -241,7 +238,7 @@ elif [ "$project" = "surveyor" ]; then # Iterate over all the template files in the directory. for template in batch-job-templates/*.tpl.json; do - template="$(basename "$template")" + template="$(basename "$template")" # Strip off the trailing .tpl for once we've formatted it. OUTPUT_BASE="$(basename "$template" .tpl.json)" FILETYPE=".json" @@ -249,21 +246,20 @@ elif [ "$project" = "surveyor" ]; then if [ "$OUTPUT_FILE" = "surveyor_dispatcher.json" ]; then perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$output_dir/$OUTPUT_FILE" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$output_dir/$OUTPUT_FILE" \ + 2>/dev/null echo "Made $output_dir/$OUTPUT_FILE" else rams="1024 4096 16384" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/$OUTPUT_BASE$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done fi @@ -272,12 +268,12 @@ elif [ "$project" = "surveyor" ]; then elif [ "$project" = "foreman" ]; then # foreman sub-project perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < environment.tpl \ - > "$output_dir/environment" \ - 2> /dev/null + "$output_dir/environment" \ + 2>/dev/null elif [ "$project" = "api" ]; then perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < environment.tpl \ - > "$output_dir/environment" \ - 2> /dev/null + "$output_dir/environment" \ + 2>/dev/null fi diff --git a/scripts/install_all.sh b/scripts/install_all.sh index 0d389712e..30a34c080 100755 --- a/scripts/install_all.sh +++ b/scripts/install_all.sh @@ -1,6 +1,6 @@ #!/bin/sh -# Exit on error +# Exit on error. set -e # Config variables @@ -8,7 +8,10 @@ TERRAFORM_VERSION="0.13.5" # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit print_description() { @@ -37,117 +40,117 @@ confirm() { printf "%s [y/N] " "$1" read -r confirmation if ! [ "$confirmation" = "y" ]; then - echo "Confirmation failure" >&2 + echo "Confirmation failure" >&2 exit 1 fi } while getopts "hv" opt; do case $opt in - h) - print_description - echo - print_usage - exit 0 - ;; - v) - OUTPUT="/dev/stdout" - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - print_usage >&2 - exit 1 - ;; + h) + print_description + echo + print_usage + exit 0 + ;; + v) + OUTPUT="/dev/stdout" + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + print_usage >&2 + exit 1 + ;; esac done # Unless output was set to stdout by the verbose flag, set it to /dev/null -# to hide the stdout of package management commands +# to hide the stdout of package management commands. if [ -z "$OUTPUT" ]; then OUTPUT="/dev/null" fi if [ -z "$INSTALL_CMD" ]; then case "$(uname)" in - "Darwin") - if ! command -v brew >/dev/null; then - confirm "Would you like to install Homebrew?" - /usr/bin/ruby -e "$(curl -fsSL https://raw.githubusercontent.com/Homebrew/install/master/install)" - fi - - INSTALL_CMD="brew install" - INSTALL_CASK_CMD="brew cask install" - BREW=true - ;; - "Linux") - if command -v apt >/dev/null; then - sudo apt-get update - INSTALL_CMD="sudo apt-get install --assume-yes" - APT=true - else - echo "Your Linux distribution is not officially supported," >&2 - echo "but it *should* be able to run the required services. You need to manually" >&2 - echo "install dependencies or give the command to install dependencies with \$INSTALL_CMD." >&2 - exit 1 - fi - ;; - *) - echo "$(uname) is an unsupported operating system." >&2 - echo "You can try to provide a package manager command with \$INSTALL_CMD," >&2 - echo "but your mileage may vary." >&2 + "Darwin") + if ! command -v brew >/dev/null; then + confirm "Would you like to install Homebrew?" + /usr/bin/ruby -e "$(curl -fsSL https://raw.githubusercontent.com/Homebrew/install/master/install)" + fi + + INSTALL_CMD="brew install" + INSTALL_CASK_CMD="brew cask install" + BREW=true + ;; + "Linux") + if command -v apt >/dev/null; then + sudo apt-get update + INSTALL_CMD="sudo apt-get install --assume-yes" + APT=true + else + echo "Your Linux distribution is not officially supported," >&2 + echo "but it *should* be able to run the required services. You need to manually" >&2 + echo "install dependencies or give the command to install dependencies with \$INSTALL_CMD." >&2 exit 1 - ;; + fi + ;; + *) + echo "$(uname) is an unsupported operating system." >&2 + echo "You can try to provide a package manager command with \$INSTALL_CMD," >&2 + echo "but your mileage may vary." >&2 + exit 1 + ;; esac fi -if ! command -v docker > /dev/null; then +if ! command -v docker >/dev/null; then echo "Installing Docker..." - # On macOS, install docker desktop with Homebrew cask - if [ $BREW ]; then - $INSTALL_CASK_CMD docker > $OUTPUT + # On macOS, install docker desktop with Homebrew cask. + if [ "$BREW" ]; then + $INSTALL_CASK_CMD docker >"$OUTPUT" else - $INSTALL_CMD docker.io > $OUTPUT || (echo "You must manually install docker" && exit 1) + $INSTALL_CMD docker.io >"$OUTPUT" || (echo "You must manually install Docker" && exit 1) - echo "Fixing docker permissions..." + echo "Fixing Docker permissions..." sudo groupadd -f docker sudo usermod -aG docker "$USER" - echo - echo "Logout and log back in to apply the permissions changes, then execute this script again." - exit 0 + echo + echo "Logout and log back in to apply the permissions changes, then execute this script again." + exit 0 fi fi -if ! command -v pip3 > /dev/null && ! [ $BREW ]; then # Don't reinstall python on macOS - echo "Installing python and pip..." - $INSTALL_CMD python3-pip > $OUTPUT || (echo "You must manually install python and pip" && exit 1) +if ! command -v pip3 >/dev/null && ! [ "$BREW" ]; then # Don't reinstall python on macOS + echo "Installing Python and pip..." + $INSTALL_CMD python3-pip >"$OUTPUT" || (echo "You must manually install Python and pip" && exit 1) fi -if ! command -v terraform > /dev/null; then - echo "Installing terraform..." - if [ $BREW ]; then - $INSTALL_CMD terraform > $OUTPUT - elif [ $APT ] || confirm "Would you like to automatically install Terraform for amd64 linux?"; then - $INSTALL_CMD unzip > $OUTPUT +if ! command -v terraform >/dev/null; then + echo "Installing Terraform..." + if [ "$BREW" ]; then + $INSTALL_CMD terraform >"$OUTPUT" + elif [ "$APT" ] || confirm "Would you like to automatically install Terraform for amd64 Linux?"; then + $INSTALL_CMD unzip >"$OUTPUT" curl -0s "https://releases.hashicorp.com/terraform/${TERRAFORM_VERSION}/terraform_${TERRAFORM_VERSION}_linux_amd64.zip" \ - > "terraform_${TERRAFORM_VERSION}_linux_amd64.zip" + >"terraform_${TERRAFORM_VERSION}_linux_amd64.zip" sudo unzip -d /usr/bin "terraform_${TERRAFORM_VERSION}_linux_amd64.zip" sudo chmod a+rx /usr/bin/terraform - rm "terraform_${TERRAFORM_VERSION}_linux_amd64.zip" + rm "terraform_${TERRAFORM_VERSION}_linux_amd64.zip" else echo "You need to manually install Terraform before continuing..." >&2 exit 1 fi fi -if ! command -v pre-commit > /dev/null; then +if ! command -v pre-commit >/dev/null; then message="Would you like to automatically install pre-commit? \ Note: This will install all the required dependencies (black, isort, etc) \ using an additional ~185MB of disk space." - if [ $APT ] || confirm "$message"; then + if [ "$APT" ] || confirm "$message"; then echo "Installing pre-commit..." - $INSTALL_CMD shellcheck > $OUTPUT + $INSTALL_CMD shellcheck >"$OUTPUT" pip3 install pre-commit pre-commit install else @@ -155,32 +158,32 @@ using an additional ~185MB of disk space." fi fi -if ! command -v jq > /dev/null; then +if ! command -v jq >/dev/null; then echo "Installing jq..." - $INSTALL_CMD jq > $OUTPUT || (echo "You must manually install jq" && exit 1) + $INSTALL_CMD jq >"$OUTPUT" || (echo "You must manually install jq" && exit 1) fi -if ! command -v ip > /dev/null; then - if [ $BREW ]; then - $INSTALL_CMD iproute2mac > $OUTPUT +if ! command -v ip >/dev/null; then + if [ "$BREW" ]; then + $INSTALL_CMD iproute2mac >"$OUTPUT" else - $INSTALL_CMD iproute2 > $OUTPUT || (echo "You must manually install iproute2" && exit 1) + $INSTALL_CMD iproute2 >"$OUTPUT" || (echo "You must manually install iproute2" && exit 1) fi fi echo "Starting postgres and installing the database..." -./run_postgres.sh > $OUTPUT -./install_db_docker.sh > $OUTPUT +./run_postgres.sh >"$OUTPUT" +./install_db_docker.sh >"$OUTPUT" echo "Starting elasticsearch and building the ES Indexes..." -./run_es.sh > $OUTPUT -./rebuild_es_index.sh > $OUTPUT +./run_es.sh >"$OUTPUT" +./rebuild_es_index.sh >"$OUTPUT" echo "Creating virtual environment..." -./create_virtualenv.sh > $OUTPUT +./create_virtualenv.sh >"$OUTPUT" echo "Run \`source dr_env/bin/activate\` to activate the virtual environment." echo "Updating common dependencies..." -# Source the virtual environment first +# Source the virtual environment first. . ../dr_env/bin/activate -./update_models.sh > $OUTPUT +./update_models.sh >"$OUTPUT" diff --git a/scripts/install_db_docker.sh b/scripts/install_db_docker.sh index 52d2e3138..8dafc2354 100755 --- a/scripts/install_db_docker.sh +++ b/scripts/install_db_docker.sh @@ -1,8 +1,45 @@ #! /bin/sh -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c "create database data_refinery" -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c "CREATE ROLE data_refinery_user WITH LOGIN PASSWORD 'data_refinery_password';" -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c 'GRANT ALL PRIVILEGES ON DATABASE data_refinery TO data_refinery_user;' -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c 'ALTER USER data_refinery_user CREATEDB;' -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c 'ALTER ROLE data_refinery_user superuser;' -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c 'CREATE EXTENSION IF NOT EXISTS hstore;' -h postgres -U postgres -d data_refinery +POSTGRES_VERSION="9.6.6" + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c "create database data_refinery" -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c "CREATE ROLE data_refinery_user WITH LOGIN PASSWORD 'data_refinery_password';" -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c 'GRANT ALL PRIVILEGES ON DATABASE data_refinery TO data_refinery_user;' -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c 'ALTER USER data_refinery_user CREATEDB;' -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c 'ALTER ROLE data_refinery_user superuser;' -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c 'CREATE EXTENSION IF NOT EXISTS hstore;' -h postgres -U postgres -d data_refinery diff --git a/scripts/kill_all_jobs.sh b/scripts/kill_all_jobs.sh index 6ef70be94..4da0eafa4 100755 --- a/scripts/kill_all_jobs.sh +++ b/scripts/kill_all_jobs.sh @@ -2,13 +2,16 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit while read -r row; do # Exporting an expansion rather than a variable, which is exactly what we want to do. # shellcheck disable=SC2163 export "${row}" -done < ../infrastructure/prod_env +done <../infrastructure/prod_env python3 kill_all_jobs.py diff --git a/scripts/make_migrations.sh b/scripts/make_migrations.sh index 25ef9e6c8..807531569 100755 --- a/scripts/make_migrations.sh +++ b/scripts/make_migrations.sh @@ -3,34 +3,44 @@ # Script for migrating the database using a Docker container so no # virtual environment is needed on the host machine. -# Exit on error +# Exit on error. set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit ./prepare_image.sh -i migrations -s common . ./common.sh + DB_HOST_IP=$(get_docker_db_ip_address) docker run \ - --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ - --add-host=database:"$DB_HOST_IP" \ - --env-file ../common/environments/local \ - --interactive \ - ccdlstaging/dr_migrations python3 manage.py makemigrations data_refinery_common + --add-host=database:"$DB_HOST_IP" \ + --env-file ../common/environments/local \ + --interactive \ + --platform linux/amd64 \ + --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ + "$DOCKERHUB_REPO/dr_migrations" \ + python3 manage.py makemigrations data_refinery_common docker run \ - --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ - --add-host=database:"$DB_HOST_IP" \ - --env-file ../common/environments/local \ - ccdlstaging/dr_migrations python3 manage.py migrate + --add-host=database:"$DB_HOST_IP" \ + --env-file ../common/environments/local \ + --platform linux/amd64 \ + --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ + "$DOCKERHUB_REPO/dr_migrations" \ + python3 manage.py migrate docker run \ - --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ - --add-host=database:"$DB_HOST_IP" \ - --env-file ../common/environments/local \ - ccdlstaging/dr_migrations python3 manage.py createcachetable + --add-host=database:"$DB_HOST_IP" \ + --env-file ../common/environments/local \ + --platform linux/amd64 \ + --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ + "$DOCKERHUB_REPO/dr_migrations" \ + python3 manage.py createcachetable diff --git a/scripts/prepare_image.sh b/scripts/prepare_image.sh index e791ce589..67f92e4d1 100755 --- a/scripts/prepare_image.sh +++ b/scripts/prepare_image.sh @@ -2,7 +2,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Import the functions in common.sh @@ -18,64 +21,71 @@ print_description() { print_options() { echo "Options:" - echo " -h Prints the help message" + echo " -h Prints the help message." echo " -i IMAGE The image to be prepared. This must be specified." echo " -s SERVICE The service to seach for a dockerfile." - echo " The default option is 'workers'" - echo " -p Pull the latest version of the image from Dockerhub" - echo " -d REPO The docker repo to pull images from." - echo " The default option is 'ccdl'" + echo " The default option is 'workers'." + echo " -d Pull the latest version of the image from Dockerhub." + echo " -u Push the built image to the Dockerhub." + echo " -r REPO The docker registry to use for pull/push actions." + echo " The default option is 'ccdlstaging'." echo echo "Examples:" echo " Build the image ccdl/dr_downloaders:" - echo " ./scripts/prepare_image.sh -i downloaders -d ccdl" + echo " ./scripts/prepare_image.sh -i downloaders -r ccdlstaging" } -while getopts "phi:d:s:" opt; do +while getopts "udhi:b:r:s:" opt; do case $opt in - i) - image=$OPTARG - ;; - d) - dockerhub_repo=$OPTARG - ;; - p) - pull="True" - ;; - s) - service=$OPTARG - ;; - h) - print_description - echo - print_options - exit 0 - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - print_options >&2 - exit 1 - ;; - :) - echo "Option -$OPTARG requires an argument." >&2 - print_options >&2 - exit 1 - ;; + b) + DOCKER_BUILDER="$OPTARG" + ;; + d) + PULL="True" + ;; + i) + IMAGE="$OPTARG" + ;; + r) + DOCKERHUB_REPO="$OPTARG" + ;; + + s) + SERVICE="$OPTARG" + ;; + u) + DOCKER_ACTION="push" + ;; + h) + print_description + echo + print_options + exit 0 + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + print_options >&2 + exit 1 + ;; + :) + echo "Option -$OPTARG requires an argument." >&2 + print_options >&2 + exit 1 + ;; esac done -if [ -z "$image" ]; then +if [ -z "$IMAGE" ]; then echo "Error: you must specify an image with -i" >&2 exit 1 fi - -if [ -z "$service" ]; then - service="workers" +if [ -z "$SERVICE" ]; then + SERVICE="workers" fi -if [ -z "$dockerhub_repo" ]; then - dockerhub_repo="ccdlstaging" +if [ -z "$DOCKERHUB_REPO" ]; then + DOCKERHUB_REPO="ccdlstaging" fi # Default to "local" for system version if we're not running in the cloud. @@ -83,43 +93,61 @@ if [ -z "$SYSTEM_VERSION" ]; then SYSTEM_VERSION="local$(date +%s)" fi +if [ -z "$DOCKER_ACTION" ]; then + DOCKER_ACTION="load" +fi + # We want to check if a test image has been built for this branch. If # it has we should use that rather than building it slowly. -image_name="$dockerhub_repo/dr_$image" +DOCKER_IMAGE="$DOCKERHUB_REPO/dr_$IMAGE" # shellcheck disable=SC2086 -if [ "$(docker_img_exists $image_name $branch_name)" ] ; then - docker pull "$image_name:$branch_name" -elif [ -n "$pull" ]; then - docker pull "$image_name" +if [ "$(docker_img_exists $IMAGE_NAME $branch_name)" ]; then + docker pull "$IMAGE_NAME:$branch_name" +elif [ -n "$PULL" ]; then + docker pull \ + --platform linux/amd64 \ + "$DOCKER_IMAGE" else echo "" - echo "Rebuilding the $image_name image." - finished=1 + echo "Rebuilding the $DOCKER_IMAGE image." + attempts=0 + finished=1 while [ $finished != 0 ] && [ $attempts -lt 3 ]; do if [ $attempts -gt 0 ]; then - echo "Failed to build $image_name, trying again." + echo "Failed to build $DOCKER_IMAGE, trying again." fi - + CURRENT_IMAGE="$DOCKER_IMAGE:$SYSTEM_VERSION" + LATEST_IMAGE="$DOCKER_IMAGE:latest" if test "$GITHUB_ACTIONS"; then - # docker needs repositories to be lowercase - CACHE_REPO="$(echo "ghrc.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]')" - CACHED_PACKAGE="$CACHE_REPO/dr_$image" - CACHE="--build-arg BUILDKIT_INLINE_CACHE=1 --cache-from $CACHED_PACKAGE" + # Docker needs repositories to be lowercase. + CACHE_REPO="$(echo "ghrc.io/$GITHUB_REPOSITORY" | + tr '[:upper:]' '[:lower:]')" + LATEST_IMAGE="$CACHE_REPO/dr_$IMAGE" + fi + + if test "$DOCKER_BUILDER"; then + docker buildx use "$DOCKER_BUILDER" fi - docker build \ - -t "$image_name" \ - -f "$service/dockerfiles/Dockerfile.$image" \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ - $CACHE . + DOCKER_BUILDKIT=1 docker buildx build \ + --"$DOCKER_ACTION" \ + --build-arg BUILDKIT_INLINE_CACHE=1 \ + --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ + --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ + --cache-from "$LATEST_IMAGE" \ + --cache-from="$CURRENT_IMAGE" \ + --file "$SERVICE/dockerfiles/Dockerfile.$IMAGE" \ + --platform linux/amd64 \ + --tag "$DOCKER_IMAGE" \ + . finished=$? - attempts=$((attempts+1)) + attempts=$((attempts + 1)) done if [ $finished != 0 ] && [ $attempts -ge 3 ]; then - echo "Could not build $image_name after three attempts." + echo "Could not build $DOCKER_IMAGE after three attempts." exit 1 fi fi diff --git a/scripts/rebuild_es_index.sh b/scripts/rebuild_es_index.sh index 30fded027..c3ac7745b 100755 --- a/scripts/rebuild_es_index.sh +++ b/scripts/rebuild_es_index.sh @@ -2,7 +2,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit ./run_manage.sh -i api_local -s api search_index --rebuild -f diff --git a/scripts/reinit_database.sh b/scripts/reinit_database.sh index 18107000d..d397c9c14 100755 --- a/scripts/reinit_database.sh +++ b/scripts/reinit_database.sh @@ -2,10 +2,12 @@ # Reintializes the database so there's no data or migrations run against it. - # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Clear it out. diff --git a/scripts/run_all_tests.sh b/scripts/run_all_tests.sh index 9372e43f2..20f7b4a14 100755 --- a/scripts/run_all_tests.sh +++ b/scripts/run_all_tests.sh @@ -7,7 +7,10 @@ set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Get access to all of the refinebio project diff --git a/scripts/run_es.sh b/scripts/run_es.sh index c300aca2f..9c2a9681a 100755 --- a/scripts/run_es.sh +++ b/scripts/run_es.sh @@ -1,13 +1,21 @@ #! /bin/sh -docker rm -f dres 2> /dev/null +docker rm -f dres 2>/dev/null -# Check if a docker database named "dres" exists, and if so just start it +# Check if a docker database named "dres" exists, and if so just start it. if [ "$(docker ps -a --filter name=dres -q)" ]; then - docker start dres > /dev/null -# Otherwise, run it with `docker run` + docker start dres >/dev/null +# Otherwise, run it with `docker run`. else - docker run --name dres -p 9200:9200 -p 9300:9300 -e "discovery.type=single-node" -e "indices.query.bool.max_clause_count=16384" -d docker.elastic.co/elasticsearch/elasticsearch:6.5.4 + docker run \ + --detach \ + --env "discovery.type=single-node" \ + --env "indices.query.bool.max_clause_count=16384" \ + --name dres \ + --platform linux/amd64 \ + --publish 9200:9200 \ + --publish 9300:9300 \ + docker.elastic.co/elasticsearch/elasticsearch:6.5.4 fi echo "Started ElasticSearch." diff --git a/scripts/run_manage.sh b/scripts/run_manage.sh index 51cc2f550..a1c1bc25d 100755 --- a/scripts/run_manage.sh +++ b/scripts/run_manage.sh @@ -6,13 +6,16 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# Import the functions in common.sh +# Import the functions in common.sh. . ./common.sh -# We need access to all of the projects +# We need access to all of the projects. cd .. print_description() { @@ -44,7 +47,7 @@ if [ "$1" = "-i" ]; then shift if [ -z "$1" ]; then echo "Error: Missing argument for -i" >&2 - echo + echo print_options >&2 exit 1 fi @@ -62,7 +65,7 @@ if [ "$1" = "-s" ]; then shift if [ -z "$1" ]; then echo "Error: Missing argument for -s" >&2 - echo + echo print_options >&2 exit 1 fi @@ -72,29 +75,36 @@ else service="foreman" fi -# Set up the data volume directory if it does not already exist +# Set up the data volume directory if it does not already exist. volume_directory="$script_directory/../api/volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" fi chmod -R a+rwX "$volume_directory" -docker build -t dr_shell -f "$service/dockerfiles/Dockerfile.$image" . +docker build \ + --file "$service/dockerfiles/Dockerfile.$image" \ + --tag dr_shell \ + . DB_HOST_IP=$(get_docker_db_ip_address) ES_HOST_IP=$(get_docker_es_ip_address) -# Only run interactively if we are on a TTY +# Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi -docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --add-host=elasticsearch:"$ES_HOST_IP" \ - --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ - --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ - --env-file "$service/environments/local" \ - --volume /tmp:/tmp \ - --volume "$volume_directory":/home/user/data_store \ - dr_shell python3 manage.py "$@" +# shellcheck disable=SC2086 +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --add-host=elasticsearch:"$ES_HOST_IP" \ + --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ + --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ + --env-file "$service/environments/local" \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + --volume /tmp:/tmp \ + $INTERACTIVE \ + dr_shell \ + python3 manage.py "$@" diff --git a/scripts/run_postgres.sh b/scripts/run_postgres.sh index 2798dc04b..6ad077bce 100755 --- a/scripts/run_postgres.sh +++ b/scripts/run_postgres.sh @@ -2,30 +2,39 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Get access to all of refinebio cd .. -# CircleCI Docker won't make this by default for some reason +# CircleCI Docker won't make this by default for some reason. # This doubly nested directory is a hacky workaround to prevent permissions issues. # Suggested here: # https://github.com/docker/for-linux/issues/380#issuecomment-436419102 VOLUMES="$script_directory/../volumes_postgres/volumes_postgres" if [ ! -d "$VOLUMES" ]; then - mkdir -p "$VOLUMES" + mkdir -p "$VOLUMES" fi # Check if a docker database named "drdb" exists, and if so just run it if [ "$(docker ps -a --filter name=drdb -q)" ]; then - docker start drdb > /dev/null - echo "Started database." -# Otherwise, install it from docker hub + docker start drdb >/dev/null + echo "Started database." +# Otherwise, install it from Docker Hub. else - # via https://hub.docker.com/_/postgres/ - # 9.6.6 is the current (as of Jan 23 2018) RDS most recent version. - # Password can be exposed to git/CI because this is only for dev/testing purposes, not real data. - echo "Installing database..." - docker run -p 5432:5432 --name drdb -v "$VOLUMES":/var/lib/postgresql/data -e POSTGRES_PASSWORD=mysecretpassword -d postgres:9.6.6 + # via https://hub.docker.com/_/postgres/ + # 9.6.6 is the current (as of Jan 23 2018) RDS most recent version. + # Password can be exposed to git/CI because this is only for dev/testing purposes, not real data. + echo "Installing database..." + docker run \ + --detach \ + --env POSTGRES_PASSWORD=mysecretpassword \ + --name drdb \ + --publish 5432:5432 \ + --volume "$VOLUMES":/var/lib/postgresql/data \ + postgres:9.6.6 fi diff --git a/scripts/run_psql_shell.sh b/scripts/run_psql_shell.sh index 9d99de96a..98939c0a0 100755 --- a/scripts/run_psql_shell.sh +++ b/scripts/run_psql_shell.sh @@ -1,2 +1,10 @@ #! /bin/sh -docker run -it -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -h postgres -U postgres -d data_refinery + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --interactive \ + --link drdb:postgres \ + --rm \ + --tty \ + postgres:9.6.6 \ + psql -h postgres -U postgres -d data_refinery diff --git a/scripts/run_shell.sh b/scripts/run_shell.sh index cfb60d00c..7760d9f2e 100755 --- a/scripts/run_shell.sh +++ b/scripts/run_shell.sh @@ -12,7 +12,10 @@ set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Import functions in common.sh @@ -28,15 +31,21 @@ if [ ! -d "$volume_directory" ]; then fi chmod -R a+rwX "$volume_directory" -docker build -t dr_shell -f foreman/dockerfiles/Dockerfile.foreman . +docker build \ + --file foreman/dockerfiles/Dockerfile.foreman \ + --tag dr_shell \ + . DB_HOST_IP=$(get_docker_db_ip_address) -docker run -it \ - --add-host="database:$DB_HOST_IP" \ - --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ - --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ - --env-file foreman/environments/local \ - --volume /tmp:/tmp \ - --volume "$volume_directory":/home/user/data_store \ - --interactive dr_shell python3 manage.py shell +docker run \ + --add-host="database:$DB_HOST_IP" \ + --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ + --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ + --env-file foreman/environments/local \ + --interactive \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + --volume /tmp:/tmp \ + dr_shell \ + python3 manage.py shell diff --git a/scripts/update_models.sh b/scripts/update_models.sh index d6648df8f..ada2c7862 100755 --- a/scripts/update_models.sh +++ b/scripts/update_models.sh @@ -1,18 +1,21 @@ #! /bin/sh -# Makes migrations and re-installs so Docker images update locally +# Makes migrations and re-installs so Docker images update locally. -# Exit on fail +# Exit on fail. set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# Get access to all of refinebio +# Get access to all of refinebio. cd .. -if ! docker ps | tail -n +2 | awk '{ print $NF }' | grep drdb > /dev/null; then +if ! docker ps | tail -n +2 | awk '{ print $NF }' | grep drdb >/dev/null; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 exit 1 @@ -25,7 +28,7 @@ if [ -z "$SYSTEM_VERSION" ]; then fi # Put this in place for common to read from. -echo "$SYSTEM_VERSION" > common/version +echo "$SYSTEM_VERSION" >common/version # Ensure there is only one distribution to copy over. rm -f common/dist/* diff --git a/scripts/update_my_docker_images.sh b/scripts/update_my_docker_images.sh index e49d1d542..d64d48694 100755 --- a/scripts/update_my_docker_images.sh +++ b/scripts/update_my_docker_images.sh @@ -1,41 +1,43 @@ #!/bin/sh -# This script is very similar to .circleci/update_docker_images.sh but it has less -# production/cloud related checks. - -# Exit on failure +# Exit on failure. set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# Get access to all of refinebio +# Get access to all of refinebio. cd .. print_description() { - echo 'This script will re-build all refine.bio docker images and push them to' - echo 'the specified Dockerhub repo.' + echo 'This script will re-build all refine.bio docker images and push ' + echo 'them to the specified Dockerhub repository.' } print_options() { - cat << EOF + cat < common/version +echo "$SYSTEM_VERSION" >common/version # Create common/dist/data-refinery-common-*.tar.gz, which is # required by the workers and data_refinery_foreman images. -## Remove old common distributions if they exist +## Remove old common distributions if they exist. rm -f common/dist/* -(cd common && python3 setup.py sdist) - -for IMG in $CCDL_WORKER_IMGS; do - image_name="$DOCKERHUB_REPO/dr_$IMG" - - echo "Building docker image: $image_name:$SYSTEM_VERSION" - # Build and push image. - docker build \ - -t "$image_name:$SYSTEM_VERSION" \ - -f "workers/dockerfiles/Dockerfile.$IMG" \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" . - docker push "$image_name:$SYSTEM_VERSION" - # Update latest version - docker tag "$image_name:$SYSTEM_VERSION" "$image_name:latest" - docker push "$image_name:latest" -done +(cd common && python3 setup.py sdist) # Run in a subshell. + +ARCH="$(uname -m)" + +if [ "$ARCH" != "$SYSTEM_ARCH" ] && [ -z "$DOCKER_BUILDER" ]; then + echo "You're building $SYSTEM_ARCH images on the $ARCH machine without " \ + "specifying a DOCKER_BUILDER. This may take a while..." + sleep 5 +fi + +for DOCKER_IMAGE in $DOCKER_IMAGES; do + case $DOCKER_IMAGE in + api) + DOCKER_FILE_PATH="api/dockerfiles/Dockerfile.api_production" + ;; + foreman) + DOCKER_FILE_PATH="foreman/dockerfiles/Dockerfile.$DOCKER_IMAGE" + ;; + *) + DOCKER_FILE_PATH="workers/dockerfiles/Dockerfile.$DOCKER_IMAGE" + ;; + esac -# Build and push Foreman image. -FOREMAN_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_foreman" -docker build \ - -t "$FOREMAN_DOCKER_IMAGE:$SYSTEM_VERSION" \ - -f foreman/dockerfiles/Dockerfile.foreman \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" . -docker push "$FOREMAN_DOCKER_IMAGE:$SYSTEM_VERSION" -# Update latest version -docker tag "$FOREMAN_DOCKER_IMAGE:$SYSTEM_VERSION" "$FOREMAN_DOCKER_IMAGE:latest" -docker push "$FOREMAN_DOCKER_IMAGE:latest" - -# Build and push API image. -API_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_api" -docker build \ - -t "$API_DOCKER_IMAGE:$SYSTEM_VERSION" \ - -f api/dockerfiles/Dockerfile.api_production \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" . -docker push "$API_DOCKER_IMAGE:$SYSTEM_VERSION" -# Update latest version -docker tag "$API_DOCKER_IMAGE:$SYSTEM_VERSION" "$API_DOCKER_IMAGE:latest" -docker push "$API_DOCKER_IMAGE:latest" + IMAGE_NAME="$DOCKERHUB_REPO/dr_$DOCKER_IMAGE" + + echo "Building $SYSTEM_ARCH $IMAGE_NAME:$SYSTEM_VERSION image from " \ + "$DOCKER_FILE_PATH." + + if test "$DOCKER_BUILDER"; then + echo "Using builder $DOCKER_BUILDER." + docker buildx use "$DOCKER_BUILDER" + fi + + DOCKER_BUILDKIT=1 docker buildx build \ + --build-arg BUILDKIT_INLINE_CACHE=1 \ + --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ + --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ + --cache-from="$IMAGE_NAME:$SYSTEM_VERSION" \ + --cache-from="$IMAGE_NAME:latest" \ + --file "$DOCKER_FILE_PATH" \ + --platform "linux/$SYSTEM_ARCH" \ + --push \ + --tag "$IMAGE_NAME:$SYSTEM_VERSION" \ + --tag "$IMAGE_NAME:latest" \ + . + + docker pull \ + --platform "linux/$SYSTEM_ARCH" \ + "$IMAGE_NAME:latest" +done diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index 6ac42f4ef..d4029d980 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.affymetrix_local b/workers/dockerfiles/Dockerfile.affymetrix_local index 7fe881ab0..52346c931 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix_local +++ b/workers/dockerfiles/Dockerfile.affymetrix_local @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_affymetrix:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 1b19485e2..de79a3320 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index b89630096..7c1ab2eab 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index 11729dba4..9c936179a 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index d9cd8818e..41b4fbb3f 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index 29bba5cd8..799e73be0 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index 3aec1395b..55da64f13 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -1,4 +1,5 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +FROM $DOCKERHUB_REPO/dr_base:latest # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] diff --git a/workers/run_command.sh b/workers/run_command.sh index da50660b1..51dc3b6c9 100755 --- a/workers/run_command.sh +++ b/workers/run_command.sh @@ -7,18 +7,18 @@ set -e while getopts "i:" opt; do case $opt in - i) - image=$OPTARG - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - exit 1 - ;; + i) + IMAGE="$OPTARG" + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + exit 1 + ;; esac done -if [ -z "$image" ]; then - image="smasher" +if [ -z "$IMAGE" ]; then + IMAGE="smasher" else shift shift @@ -26,7 +26,10 @@ fi # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to @@ -47,16 +50,19 @@ fi chmod -R a+rwX "$volume_directory" . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) -./scripts/prepare_image.sh -i "$image" -s workers -image_name=ccdlstaging/dr_"$image" +./scripts/prepare_image.sh -i "$IMAGE" -s workers docker run \ - --add-host=database:"$DB_HOST_IP" \ - --env-file workers/environments/local \ - --env AWS_ACCESS_KEY_ID \ - --env AWS_SECRET_ACCESS_KEY \ - --volume "$volume_directory":/home/user/data_store \ - --link drdb:postgres \ - -it "$image_name" bash -c "$@" + --add-host=database:"$DB_HOST_IP" \ + --env AWS_ACCESS_KEY_ID \ + --env AWS_SECRET_ACCESS_KEY \ + --env-file workers/environments/local \ + --ineractive \ + --link drdb:postgres \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + "$DOCKERHUB_REPO/dr_$IMAGE" \ + bash -c "$@" diff --git a/workers/run_janitor.sh b/workers/run_janitor.sh index 27c6ed6f5..70d9dcbde 100755 --- a/workers/run_janitor.sh +++ b/workers/run_janitor.sh @@ -1,19 +1,20 @@ #!/bin/sh -# the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +# The directory it lives in. +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. ./scripts/prepare_image.sh -i smasher -image_name="ccdlstaging/dr_smasher" - -volume_directory="$script_directory/volume" . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) AWS_ACCESS_KEY_ID="$(~/bin/aws configure get default.aws_access_key_id)" @@ -22,13 +23,15 @@ AWS_SECRET_ACCESS_KEY="$(~/bin/aws configure get default.aws_secret_access_key)" export AWS_SECRET_ACCESS_KEY docker run \ - -it \ - -m 500m \ - --add-host=database:"$DB_HOST_IP" \ - --env-file workers/environments/local \ - --env AWS_ACCESS_KEY_ID \ - --env AWS_SECRET_ACCESS_KEY \ - --entrypoint ./manage.py \ - --volume "$volume_directory":/home/user/data_store \ - --link drdb:postgres \ - "$image_name" run_janitor + --add-host=database:"$DB_HOST_IP" \ + --entrypoint ./manage.py \ + --env AWS_ACCESS_KEY_ID \ + --env AWS_SECRET_ACCESS_KEY \ + --env-file workers/environments/local \ + --interactive \ + --link drdb:postgres \ + --memory 500m \ + --tty \ + --volume "$script_directory/volume":/home/user/data_store \ + "$DOCKERHUB_REPO/dr_smasher" \ + run_janitor diff --git a/workers/run_job.sh b/workers/run_job.sh index 7342103bc..4756908b3 100755 --- a/workers/run_job.sh +++ b/workers/run_job.sh @@ -1,67 +1,70 @@ #!/bin/bash -# Script for running a django management command to test the worker. +# Script for running a Django management command to test the worker. while getopts "hi:" opt; do case $opt in - i) - image=$OPTARG - ;; - h) - echo "Runs a downloader or processor job. The following arguments are supported:" - echo "-h : Print this help message and exit." - echo "-i : The image to use. Options are:" - echo " downloaders (default)" - echo " salmon" - echo " transcriptome" - echo " no_op" - echo " downloaders" - echo " illumina" - echo " affymetrix" - echo " : What kind of job to run." - echo " Must be either 'run_downloader_job' or 'run_processor_job'." - echo "--job-name= : The type of job to run." - echo " For processor jobs, options are:" - echo " AFFY_TO_PCL" - echo " AGILENT_TWOCOLOR_TO_PCL" - echo " SALMON" - echo " ILLUMINA_TO_PCL" - echo " TRANSCRIPTOME_INDEX_LONG" - echo " TRANSCRIPTOME_INDEX_SHORT" - echo " NO_OP" - echo " For downloader jobs, options are:" - echo " ARRAY_EXPRESS" - echo " SRA" - echo " TRANSCRIPTOME_INDEX" - echo " GEO" - echo "--job-id= : The id of the job you want to run. Must already exist in the database." - echo "" - echo "Note that the must correspond to the ." - echo " AGILENT_TWOCOLOR_TO_PCL is a special case because it requires the 'affymetrix' image." - echo "" - echo "Examples:" - echo " ./workers/run_job.sh run_downloader_job --job-name=SRA --job-id=12345" - echo " ./workers/run_job.sh -i affymetrix run_processor_job --job-name=AGILENT_TWOCOLOR_TO_PCL --job-id=54321" - exit 0 - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - exit 1 - ;; - :) - echo "Option -$OPTARG requires an argument." >&2 - exit 1 - ;; + i) + IMAGE="$OPTARG" + ;; + h) + echo "Runs a downloader or processor job. The following arguments are supported:" + echo "-h : Print this help message and exit." + echo "-i : The image to use. Options are:" + echo " downloaders (default)" + echo " salmon" + echo " transcriptome" + echo " no_op" + echo " downloaders" + echo " illumina" + echo " affymetrix" + echo " : What kind of job to run." + echo " Must be either 'run_downloader_job' or 'run_processor_job'." + echo "--job-name= : The type of job to run." + echo " For processor jobs, options are:" + echo " AFFY_TO_PCL" + echo " AGILENT_TWOCOLOR_TO_PCL" + echo " SALMON" + echo " ILLUMINA_TO_PCL" + echo " TRANSCRIPTOME_INDEX_LONG" + echo " TRANSCRIPTOME_INDEX_SHORT" + echo " NO_OP" + echo " For downloader jobs, options are:" + echo " ARRAY_EXPRESS" + echo " SRA" + echo " TRANSCRIPTOME_INDEX" + echo " GEO" + echo "--job-id= : The id of the job you want to run. Must already exist in the database." + echo "" + echo "Note that the must correspond to the ." + echo " AGILENT_TWOCOLOR_TO_PCL is a special case because it requires the 'affymetrix' image." + echo "" + echo "Examples:" + echo " ./workers/run_job.sh run_downloader_job --job-name=SRA --job-id=12345" + echo " ./workers/run_job.sh -i affymetrix run_processor_job --job-name=AGILENT_TWOCOLOR_TO_PCL --job-id=54321" + exit 0 + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + exit 1 + ;; + :) + echo "Option -$OPTARG requires an argument." >&2 + exit 1 + ;; esac done -if [[ -z "$image" ]]; then - image="downloaders" +if [[ -z "$IMAGE" ]]; then + IMAGE="downloaders" fi # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to @@ -69,12 +72,12 @@ cd "$script_directory" || exit cd .. # Agilent uses the same image as affymetrix -if [[ "$image" == "affymetrix" || "$image" == "agilent" ]]; then - ./scripts/prepare_image.sh -p -i affymetrix - image_name="ccdlstaging/dr_affymetrix" +if [[ "$IMAGE" == "affymetrix" || "$IMAGE" == "agilent" ]]; then + ./scripts/prepare_image.sh -d -i affymetrix + IMAGE_NAME="$DOCKERHUB_REPO/dr_affymetrix" else - ./scripts/prepare_image.sh -i "$image" - image_name="ccdlstaging/dr_$image" + ./scripts/prepare_image.sh -i "$IMAGE" + IMAGE_NAME="$DOCKERHUB_REPO/dr_$IMAGE" fi volume_directory="$script_directory/volume" @@ -83,16 +86,18 @@ if [ ! -d "$volume_directory" ]; then fi chmod -R a+rwX "$volume_directory" -source scripts/common.sh +. scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) docker run \ - -it \ - --add-host=database:"$DB_HOST_IP" \ - --env-file workers/environments/local \ - --env AWS_ACCESS_KEY_ID \ - --env AWS_SECRET_ACCESS_KEY \ - --entrypoint ./manage.py \ - --volume "$volume_directory":/home/user/data_store \ - --link drdb:postgres \ - "$image_name" "${@: -3}" "${@: -2}" "${@: -1}" + --add-host=database:"$DB_HOST_IP" \ + --entrypoint ./manage.py \ + --env AWS_ACCESS_KEY_ID \ + --env AWS_SECRET_ACCESS_KEY \ + --env-file workers/environments/local \ + --interactive \ + --link drdb:postgres \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + "$IMAGE_NAME" "${@: -3}" "${@: -2}" "${@: -1}" diff --git a/workers/run_tests.sh b/workers/run_tests.sh index 3e03e2ada..45be0fee1 100755 --- a/workers/run_tests.sh +++ b/workers/run_tests.sh @@ -3,7 +3,7 @@ # Script for executing Django PyUnit tests within a Docker container. -# Exit on failure +# Exit on failure. set -e print_description() { @@ -20,47 +20,49 @@ print_options() { while getopts ":t:h" opt; do case $opt in - t) - tag=$OPTARG - ;; - h) - print_description - echo - print_options - exit 0 - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - print_options >&2 - exit 1 - ;; - :) - echo "Option -$OPTARG requires an argument." >&2 - print_options >&2 - exit 1 - ;; + t) + tag="$OPTARG" + ;; + h) + print_description + echo + print_options + exit 0 + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + print_options >&2 + exit 1 + ;; + :) + echo "Option -$OPTARG requires an argument." >&2 + print_options >&2 + exit 1 + ;; esac done # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to +# However, in order to give Docker access to all the code we have to # move up a level cd .. -# Ensure that postgres is running +# Ensure that Postgres is running. if ! [ "$(docker ps --filter name=drdb -q)" ]; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 exit 1 fi -volume_directory="$script_directory/test_volume" - test_data_repo="https://s3.amazonaws.com/data-refinery-test-assets" +volume_directory="$script_directory/test_volume" if [ -z "$tag" ] || [ "$tag" = "salmon" ]; then # Download "salmon quant" test data The `newer` file was to @@ -77,11 +79,11 @@ if [ -z "$tag" ] || [ "$tag" = "salmon" ]; then rm "$volume_directory"/salmon_tests.tar.gz fi - # salmontools test data + # SalmonTools test data. salmontools_test_zip="$test_data_repo/salmontools_test_data.tar.gz" salmontools_test_dir="$volume_directory/salmontools" - # Clean the test data directory + # Clean the test data directory. rm -rf "$salmontools_test_dir" mkdir -p "$salmontools_test_dir" @@ -102,21 +104,21 @@ if [ -z "$tag" ] || [ "$tag" = "salmon" ]; then mkdir -p "$rna_seq_test_raw_dir" echo "Downloading $read_1_name for Salmon tests." wget -q -O "$rna_seq_test_data_1" \ - "$test_data_repo/$read_1_name" + "$test_data_repo/$read_1_name" echo "Downloading $read_2_name for Salmon tests." wget -q -O "$rna_seq_test_data_2" \ - "$test_data_repo/$read_2_name" + "$test_data_repo/$read_2_name" fi if [ ! -e "$dotsra" ]; then mkdir -p "$rna_seq_test_raw_dir" echo "Downloading $dotsra_name for Salmon tests." wget -q -O "$dotsra" \ - "$test_data_repo/$dotsra_name" + "$test_data_repo/$dotsra_name" fi fi if [ -z "$tag" ] || [ "$tag" = "affymetrix" ]; then - # Make sure CEL for test is downloaded from S3 + # Make sure CEL for test is downloaded from S3. cel_name="GSM1426071_CD_colon_active_1.CEL" cel_name2="GSM45588.CEL" cel_name3="GSM1364667_U_110208_7-02-10_S2.CEL" @@ -135,35 +137,35 @@ if [ -z "$tag" ] || [ "$tag" = "affymetrix" ]; then mkdir -p "$cel_test_raw_dir" echo "Downloading CEL for tests." wget -q -O "$cel_test_data_1" \ - "$test_data_repo/$cel_name" + "$test_data_repo/$cel_name" fi if [ ! -e "$cel_test_data_2" ]; then echo "Downloading Non-Brainarray CEL for tests." wget -q -O "$cel_test_data_2" \ - "$test_data_repo/$cel_name2" + "$test_data_repo/$cel_name2" fi if [ ! -e "$cel_test_data_3" ]; then echo "Downloading Huex Brain Array CEL for tests." wget -q -O "$cel_test_data_3" \ - "$test_data_repo/$cel_name3" + "$test_data_repo/$cel_name3" fi if [ ! -e "$pcl_test_data_1" ]; then mkdir -p "$pcl_test_dir" echo "Downloading pre-computed PCL for tests." wget -q -O "$pcl_test_data_1" \ - "$test_data_repo/$pcl_name" + "$test_data_repo/$pcl_name" fi if [ ! -e "$pcl_test_data_2" ]; then mkdir -p "$pcl_test_dir" echo "Downloading pre-computed Non-Brainarray PCL for tests." wget -q -O "$pcl_test_data_2" \ - "$test_data_repo/$pcl_name2" + "$test_data_repo/$pcl_name2" fi if [ ! -e "$pcl_test_data_3" ]; then mkdir -p "$pcl_test_dir" echo "Downloading pre-computed Huex Brain Array PCL for tests." wget -q -O "$pcl_test_data_3" \ - "$test_data_repo/$pcl_name3" + "$test_data_repo/$pcl_name3" fi fi @@ -176,14 +178,14 @@ if [ -z "$tag" ] || [ "$tag" = "transcriptome" ]; then mkdir -p "$tx_index_test_raw_dir" echo "Downloading fasta file for Transcriptome Index tests." wget -q -O "$tx_index_test_raw_dir/$fasta_file" \ - "$test_data_repo/$fasta_file" + "$test_data_repo/$fasta_file" fi gtf_file="aegilops_tauschii_short.gtf.gz" if [ ! -e "$tx_index_test_raw_dir/$gtf_file" ]; then mkdir -p "$tx_index_test_raw_dir" echo "Downloading gtf file for Transcriptome Index tests." wget -q -O "$tx_index_test_raw_dir/$gtf_file" \ - "$test_data_repo/$gtf_file" + "$test_data_repo/$gtf_file" fi tx_index_test_raw_dir2="$volume_directory/raw/TEST/TRANSCRIPTOME_INDEX/" gtf_file2="Homo_sapiens_testdata.gtf" @@ -191,7 +193,7 @@ if [ -z "$tag" ] || [ "$tag" = "transcriptome" ]; then mkdir -p "$tx_index_test_raw_dir2" echo "Downloading second gtf file for Transcriptome Index tests." wget -q -O "$tx_index_test_raw_dir2/$gtf_file2" \ - "$test_data_repo/$gtf_file2" + "$test_data_repo/$gtf_file2" fi fi @@ -210,18 +212,17 @@ GSE41355_non-normalized.txt GSE100301_non-normalized.txt' "$test_data_repo/$ilu_file" fi - i=$(( i + 1 )) + i=$((i + 1)) done unset i - ilu_test_ref_dir="$volume_directory/raw/TEST/ILLUMINA/reference" ilu_ref_file="Ad-Cre-2.AVG_Signal.tsv" if [ ! -e "$ilu_test_ref_dir/$ilu_ref_file" ]; then mkdir -p "$ilu_test_ref_dir" echo "Downloading Illumin reference file for Illumina tests." wget -q -O "$ilu_test_ref_dir/$ilu_ref_file" \ - "$test_data_repo/$ilu_ref_file" + "$test_data_repo/$ilu_ref_file" fi fi @@ -233,7 +234,7 @@ if [ -z "$tag" ] || [ "$tag" = "agilent" ]; then mkdir -p "$at_test_raw_dir" echo "Downloading Agilent file for A2C tests." wget -q -O "$at_test_raw_dir/$at_file" \ - "$test_data_repo/$at_file" + "$test_data_repo/$at_file" fi fi if [ -z "$tag" ] || [ "$tag" = "no_op" ]; then @@ -243,37 +244,37 @@ if [ -z "$tag" ] || [ "$tag" = "no_op" ]; then mkdir -p "$no_test_raw_dir" echo "Downloading NOOP file1." wget -q -O "$no_test_raw_dir/$no_file1" \ - "$test_data_repo/$no_file1" + "$test_data_repo/$no_file1" fi no_file2="GSM1234847_sample_table.txt" if [ ! -e "$no_test_raw_dir/$no_file2" ]; then mkdir -p "$no_test_raw_dir" echo "Downloading NOOP file2." wget -q -O "$no_test_raw_dir/$no_file2" \ - "$test_data_repo/$no_file2" + "$test_data_repo/$no_file2" fi no_file3="GSM1234847_sample_table_headerless.txt" if [ ! -e "$no_test_raw_dir/$no_file3" ]; then mkdir -p "$no_test_raw_dir" echo "Processing NOOP file3." - tail -n +2 "$no_test_raw_dir/$no_file2" > "$no_test_raw_dir/$no_file3" + tail -n +2 "$no_test_raw_dir/$no_file2" >"$no_test_raw_dir/$no_file3" fi no_file4="GSM1089291-tbl-1.txt" if [ ! -e "$no_test_raw_dir/$no_file4" ]; then mkdir -p "$no_test_raw_dir" echo "Downloading NOOP file4." wget -q -O "$no_test_raw_dir/$no_file4" \ - "$test_data_repo/$no_file4" + "$test_data_repo/$no_file4" fi no_file5="GSM1089291-tbl-1-modified.txt" if [ ! -e "$no_test_raw_dir/$no_file5" ]; then mkdir -p "$no_test_raw_dir" echo "Downloading NOOP file5." wget -q -O "$no_test_raw_dir/$no_file5" \ - "$test_data_repo/$no_file5" + "$test_data_repo/$no_file5" fi - # Reference files + # Reference files. no_test_exp_dir="$volume_directory/TEST/NO_OP/EXPECTED" no_test_exp_files='gene_converted_GSM557500-tbl-1.txt GSM269747.PCL gene_converted_GSM1234847-tbl-1.txt gene_converted_GSM1089291-tbl-1.txt' mkdir -p "$no_test_exp_dir" @@ -283,16 +284,16 @@ if [ -z "$tag" ] || [ "$tag" = "no_op" ]; then if ! [ -e "$no_test_exp_dir/$no_test_exp_file" ]; then echo "Downloading NOOP expected file$i." wget -O "$no_test_exp_dir/$no_test_exp_file" \ - "$test_data_repo/$no_test_exp_file" + "$test_data_repo/$no_test_exp_file" fi - i=$(( i + 1 )) + i=$((i + 1)) done unset i fi if [ -z "$tag" ] || [ "$tag" = "smasher" ] || [ "$tag" = "compendia" ]; then - # Make sure PCL for test is downloaded from S3 + # Make sure PCL for test is downloaded from S3. pcl_name="GSM1237810_T09-1084.PCL" pcl_name2="GSM1237812_S97-PURE.PCL" pcl_name3="GSM1238108-tbl-1.txt" @@ -334,101 +335,101 @@ if [ -z "$tag" ] || [ "$tag" = "smasher" ] || [ "$tag" = "compendia" ]; then mkdir -p "$pcl_test_raw_dir" echo "Downloading PCL for tests." wget -q -O "$pcl_test_data_1" \ - "$test_data_repo/$pcl_name" + "$test_data_repo/$pcl_name" fi if [ ! -e "$pcl_test_data_2" ]; then echo "Downloading PCL2 for tests." wget -q -O "$pcl_test_data_2" \ - "$test_data_repo/$pcl_name2" + "$test_data_repo/$pcl_name2" fi if [ ! -e "$pcl_test_data_3" ]; then echo "Downloading PCL3 for tests." wget -q -O "$pcl_test_data_3" \ - "$test_data_repo/$pcl_name3" + "$test_data_repo/$pcl_name3" fi if [ ! -e "$pcl_test_data_4" ]; then echo "Downloading PCL4 for tests." wget -q -O "$pcl_test_data_4" \ - "$test_data_repo/$pcl_name4" + "$test_data_repo/$pcl_name4" fi if [ ! -e "$pcl_test_data_5" ]; then echo "Downloading PCL5 for tests." wget -q -O "$pcl_test_data_5" \ - "$test_data_repo/$pcl_name5" + "$test_data_repo/$pcl_name5" fi if [ ! -e "$pcl_test_data_6" ]; then echo "Downloading PCL6 for tests." wget -q -O "$pcl_test_data_6" \ - "$test_data_repo/$pcl_name6" + "$test_data_repo/$pcl_name6" fi if [ ! -e "$pcl_test_data_7" ]; then echo "Downloading PCL7 for tests." wget -q -O "$pcl_test_data_7" \ - "$test_data_repo/$pcl_name7" + "$test_data_repo/$pcl_name7" fi if [ ! -e "$pcl_test_data_gs1" ]; then echo "Downloading PCLGS1 for tests." wget -q -O "$pcl_test_data_gs1" \ - "$test_data_repo/$pcl_name_gs1" + "$test_data_repo/$pcl_name_gs1" fi if [ ! -e "$pcl_test_data_gs2" ]; then echo "Downloading PCLGS2 for tests." wget -q -O "$pcl_test_data_gs2" \ - "$test_data_repo/$pcl_name_gs2" + "$test_data_repo/$pcl_name_gs2" fi if [ ! -e "$pcl_test_data_ts1" ]; then echo "Downloading PCLTS1 for tests." wget -q -O "$pcl_test_data_ts1" \ - "$test_data_repo/$pcl_name_ts1" + "$test_data_repo/$pcl_name_ts1" fi if [ ! -e "$pcl_test_data_ts2" ]; then echo "Downloading PCLTS2 for tests." wget -q -O "$pcl_test_data_ts2" \ - "$test_data_repo/$pcl_name_ts2" + "$test_data_repo/$pcl_name_ts2" fi if [ ! -e "$pcl_test_data_ta1" ]; then echo "Downloading PCLTA1 for tests." wget -q -O "$pcl_test_data_ta1" \ - "$test_data_repo/$pcl_name_ta1" + "$test_data_repo/$pcl_name_ta1" fi if [ ! -e "$bad_test_data_1" ]; then mkdir -p "$bad_test_raw_dir" echo "Downloading Bad PCL for tests." wget -q -O "$bad_test_data_1" \ - "$test_data_repo/$bad_name" + "$test_data_repo/$bad_name" fi if [ ! -e "$bad_test_data_2" ]; then mkdir -p "$bad_test_raw_dir" echo "Downloading Bad PCL for tests." wget -q -O "$bad_test_data_2" \ - "$test_data_repo/$bad_name2" + "$test_data_repo/$bad_name2" fi if [ ! -e "$bad_test_data_3" ]; then mkdir -p "$bad_test_raw_dir" echo "Downloading Bad PCL for tests." wget -q -O "$bad_test_data_3" \ - "$test_data_repo/$bad_name3" + "$test_data_repo/$bad_name3" fi if [ ! -e "$quant_test_data_1" ]; then mkdir -p "$quant_test_raw_dir" echo "Downloading Quant files for tests." wget -q -O "$quant_test_data_1" \ - "$test_data_repo/$quant_name" + "$test_data_repo/$quant_name" fi if [ ! -e "$quant_test_data_2" ]; then mkdir -p "$quant_test_raw_dir" echo "Downloading Quant files for tests." wget -q -O "$quant_test_data_2" \ - "$test_data_repo/$quant_name_2" + "$test_data_repo/$quant_name_2" fi # Mock out the AWS keys since we use VCR to mock out the request with these - # as the AWS credentials + # as the AWS credentials. export AWS_ACCESS_KEY_ID=XXX export AWS_SECRET_ACCESS_KEY=XXX fi if [ -z "$tag" ] || [ "$tag" = "qn" ]; then - # Make sure PCL for test is downloaded from S3 + # Make sure PCL for test is downloaded from S3. qn_name="1.tsv" qn_test_raw_dir="$volume_directory/QN" qn_test_data_1="$qn_test_raw_dir/$qn_name" @@ -436,7 +437,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_1" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="2.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -445,7 +446,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_2" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="3.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -454,7 +455,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_3" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="4.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -463,7 +464,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_4" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="5.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -472,7 +473,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_5" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="6.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -481,7 +482,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_6" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="7.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -490,7 +491,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_7" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi fi if [ -z "$tag" ] || [ "$tag" = "compendia" ]; then @@ -522,7 +523,7 @@ if [ -z "$tag" ] || [ "$tag" = "compendia" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for compendia tests." wget -q -O "$qn_test_data_1" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi fi @@ -533,47 +534,50 @@ DB_HOST_IP=$(get_docker_db_ip_address) chmod -R a+rwX "$volume_directory" worker_images="salmon transcriptome no_op downloaders smasher illumina agilent affymetrix qn affymetrix_local janitor compendia" - for image in $worker_images; do if [ -z "$tag" ] || [ "$tag" = "$image" ]; then if [ "$image" = "agilent" ] || [ "$image" = "affymetrix" ]; then - # Agilent uses the same docker image as Affymetrix - ./scripts/prepare_image.sh -p -i affymetrix -s workers - ./scripts/prepare_image.sh -i affymetrix_local -d ccdlstaging - docker tag ccdlstaging/dr_affymetrix_local:latest ccdlstaging/dr_affymetrix:latest - image_name=ccdlstaging/dr_affymetrix + # Agilent uses the same docker image as Affymetrix. + ./scripts/prepare_image.sh -d -i affymetrix -s workers + ./scripts/prepare_image.sh -i affymetrix_local -r ccdlstaging + docker tag "$DOCKERHUB_REPO/dr_affymetrix_local:latest" "$DOCKERHUB_REPO/dr_affymetrix:latest" + image_name="$DOCKERHUB_REPO/dr_affymetrix" elif [ "$tag" = "qn" ]; then ./scripts/prepare_image.sh -i smasher -s workers - image_name=ccdlstaging/dr_smasher + image_name="$DOCKERHUB_REPO/dr_smasher" elif [ "$tag" = "janitor" ]; then ./scripts/prepare_image.sh -i smasher -s workers - image_name=ccdlstaging/dr_smasher + image_name="$DOCKERHUB_REPO/dr_smasher" else ./scripts/prepare_image.sh -i "$image" -s workers - image_name=ccdlstaging/dr_$image + image_name="$DOCKERHUB_REPO/dr_$image" fi - # Strip out tag argument + # Strip out tag argument. # shellcheck disable=2001 args_without_tag="$(echo "$@" | sed "s/-t $tag//")" # shellcheck disable=2086 test_command="$(run_tests_with_coverage --tag="$image" $args_without_tag)" - # Only run interactively if we are on a TTY + # Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi echo "Running tests with the following command:" echo "$test_command" - docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --env-file workers/environments/test \ - --env AWS_ACCESS_KEY_ID \ - --env AWS_SECRET_ACCESS_KEY \ - --memory=5G \ - --platform linux/amd64 \ - --volume "$volume_directory":/home/user/data_store \ - "$image_name" bash -c "$test_command" + # shellcheck disable=SC2086 + docker run \ + --add-host=database:"$DB_HOST_IP" \ + --env AWS_ACCESS_KEY_ID \ + --env AWS_SECRET_ACCESS_KEY \ + --env-file workers/environments/test \ + --memory=5G \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + $INTERACTIVE \ + "$image_name" \ + bash -c "$test_command" fi done From 8656fd93c1ae811d8fe1623273e8a23eca62cde0 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 10 May 2023 12:49:44 -0700 Subject: [PATCH 124/220] Change default registry setting --- scripts/common.sh | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/scripts/common.sh b/scripts/common.sh index 07191400e..4f5f46bd9 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -5,8 +5,11 @@ export ALL_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders foreman api" # Sometimes we only want to work with the worker images. export WORKER_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders" + # Default Docker registry. -export DOCKERHUB_REPO="ccdlstaging" +if [ -z "$DOCKERHUB_REPO" ]; then + export DOCKERHUB_REPO="ccdlstaging" +fi get_docker_db_ip_address() { docker inspect -f '{{range .NetworkSettings.Networks}}{{.IPAddress}}{{end}}' drdb 2>/dev/null From 245ef73421c747e13df97efeefcddb181fea8021 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 10 May 2023 15:24:49 -0700 Subject: [PATCH 125/220] Update scripts --- .github/scripts/cleanup_instance.sh | 9 ++++++++- .github/scripts/filter_tests.sh | 6 +++--- .github/scripts/post_deploy_cleanup.sh | 3 ++- .github/scripts/pull_docker_images.sh | 2 +- .github/scripts/remote_deploy.sh | 9 ++++----- .github/scripts/run_terraform.sh | 12 +++++------ .github/scripts/slackpost_deploy.sh | 19 ++++++++--------- .github/scripts/slackpost_end_to_end.sh | 19 ++++++++--------- .github/scripts/update_docker_img.sh | 27 +++++++++++++------------ .github/scripts/upload_test_coverage.sh | 11 ++++------ scripts/prepare_image.sh | 23 ++++++++++++--------- scripts/update_my_docker_images.sh | 6 +++--- 12 files changed, 73 insertions(+), 73 deletions(-) diff --git a/.github/scripts/cleanup_instance.sh b/.github/scripts/cleanup_instance.sh index d270e1582..d359f6966 100755 --- a/.github/scripts/cleanup_instance.sh +++ b/.github/scripts/cleanup_instance.sh @@ -13,7 +13,14 @@ sudo apt-get remove -y '^ghc-8.*' sudo apt-get remove -y '^dotnet-.*' sudo apt-get remove -y '^llvm-.*' sudo apt-get remove -y 'php.*' -sudo apt-get remove -y azure-cli google-cloud-sdk hhvm google-chrome-stable firefox powershell mono-devel +sudo apt-get remove -y \ + azure-cli \ + firefox \ + google-chrome-stable \ + google-cloud-sdk \ + hhvm \ + mono-devel \ + powershell sudo apt-get autoremove -y sudo apt-get clean diff --git a/.github/scripts/filter_tests.sh b/.github/scripts/filter_tests.sh index fca0caec3..88a3dfff5 100755 --- a/.github/scripts/filter_tests.sh +++ b/.github/scripts/filter_tests.sh @@ -1,14 +1,14 @@ #!/bin/bash -# Exit on failure +# Exit on failure. set -e git log --format=oneline -n 1 "$GITHUB_SHA" if [[ $(git log --format=oneline -n 1 "$GITHUB_SHA") = *"noslow"* ]]; then - echo "Skipping slow tests.."; + echo "Skipping slow tests..." ./workers/run_tests.sh --exclude-tag=slow "$@" else - echo "Running all tests.."; + echo "Running all tests..." ./workers/run_tests.sh "$@" fi diff --git a/.github/scripts/post_deploy_cleanup.sh b/.github/scripts/post_deploy_cleanup.sh index 467cbc50e..9901a3b26 100755 --- a/.github/scripts/post_deploy_cleanup.sh +++ b/.github/scripts/post_deploy_cleanup.sh @@ -8,4 +8,5 @@ ssh -o StrictHostKeyChecking=no \ -o ServerAliveInterval=15 \ -i infrastructure/data-refinery-key.pem \ - ubuntu@"${DEPLOY_IP_ADDRESS}" "cd refinebio && git clean -f" + "ubuntu@${DEPLOY_IP_ADDRESS}" \ + "cd refinebio && git clean -f" diff --git a/.github/scripts/pull_docker_images.sh b/.github/scripts/pull_docker_images.sh index c691a0b41..4d59d04bd 100755 --- a/.github/scripts/pull_docker_images.sh +++ b/.github/scripts/pull_docker_images.sh @@ -10,6 +10,6 @@ fi for image in $IMAGES; do PACKAGE="$REPO/dr_$image" - # Only pull the package if it already exists + # Only pull the package if it already exists. (docker pull "$PACKAGE" && docker tag "$PACKAGE" "ccdlstaging/dr_$image") || true done diff --git a/.github/scripts/remote_deploy.sh b/.github/scripts/remote_deploy.sh index a22618b6b..c82697a7a 100755 --- a/.github/scripts/remote_deploy.sh +++ b/.github/scripts/remote_deploy.sh @@ -18,11 +18,10 @@ # - AWS_ACCESS_KEY_ID -- The AWS key id to use when interacting with AWS. # - AWS_SECRET_ACCESS_KEY -- The AWS secret key to use when interacting with AWS. - -echo "$INSTANCE_SSH_KEY" > infrastructure/data-refinery-key.pem +echo "$INSTANCE_SSH_KEY" >infrastructure/data-refinery-key.pem chmod 600 infrastructure/data-refinery-key.pem -run_on_deploy_box () { +run_on_deploy_box() { # shellcheck disable=SC2029 ssh -o StrictHostKeyChecking=no \ -o ServerAliveInterval=15 \ @@ -32,7 +31,7 @@ run_on_deploy_box () { # Create file containing local env vars that are needed for deploy. rm -f env_vars -cat >> env_vars <>env_vars < ~/refinebio/common/version +echo "$CI_TAG" >~/refinebio/common/version # Create ~/refinebio/common/dist/data-refinery-common-*.tar.gz, which is # required by the workers and data_refinery_foreman images. @@ -36,9 +36,10 @@ for IMAGE in $WORKER_IMAGES; do echo "Building docker image: $image_name:$CI_TAG" # Build and push image. We use the CI_TAG as the system version. docker build \ - -t "$image_name:$CI_TAG" \ - -f "workers/dockerfiles/Dockerfile.$IMAGE" \ - --build-arg SYSTEM_VERSION="$CI_TAG" . + --build-arg SYSTEM_VERSION="$CI_TAG" \ + --file "workers/dockerfiles/Dockerfile.$IMAGE" \ + --tag "$image_name:$CI_TAG" \ + . docker push "$image_name:$CI_TAG" # Update latest version. docker tag "$image_name:$CI_TAG" "$image_name:latest" @@ -56,10 +57,10 @@ if docker_img_exists "$FOREMAN_DOCKER_IMAGE" "$CI_TAG"; then else # Build and push image. We use the CI_TAG as the system version. docker build \ - --build-arg SYSTEM_VERSION="$CI_TAG" \ - --file foreman/dockerfiles/Dockerfile.foreman \ - --tag "$FOREMAN_DOCKER_IMAGE:$CI_TAG" \ - . + --build-arg SYSTEM_VERSION="$CI_TAG" \ + --file foreman/dockerfiles/Dockerfile.foreman \ + --tag "$FOREMAN_DOCKER_IMAGE:$CI_TAG" \ + . docker push "$FOREMAN_DOCKER_IMAGE:$CI_TAG" # Update latest version. docker tag "$FOREMAN_DOCKER_IMAGE:$CI_TAG" "$FOREMAN_DOCKER_IMAGE:latest" @@ -73,10 +74,10 @@ if docker_img_exists "$API_DOCKER_IMAGE" "$CI_TAG"; then else # Build and push image. We use the CI_TAG as the system version. docker build \ - --build-arg SYSTEM_VERSION="$CI_TAG" \ - --file api/dockerfiles/Dockerfile.api_production \ - --tag "$API_DOCKER_IMAGE:$CI_TAG" \ - . + --build-arg SYSTEM_VERSION="$CI_TAG" \ + --file api/dockerfiles/Dockerfile.api_production \ + --tag "$API_DOCKER_IMAGE:$CI_TAG" \ + . docker push "$API_DOCKER_IMAGE:$CI_TAG" # Update latest version. docker tag "$API_DOCKER_IMAGE:$CI_TAG" "$API_DOCKER_IMAGE:latest" diff --git a/.github/scripts/upload_test_coverage.sh b/.github/scripts/upload_test_coverage.sh index 44a009cee..12068f485 100755 --- a/.github/scripts/upload_test_coverage.sh +++ b/.github/scripts/upload_test_coverage.sh @@ -3,14 +3,12 @@ # Script to upload code coverage project=$1 -if [[ $project == "" ]] -then +if [[ $project == "" ]]; then echo "No project specified" exit 1 fi -if [[ $project == "workers" ]] -then +if [[ $project == "workers" ]]; then # the workers project uses it's own test_volume directory test_volume="workers/test_volume" else @@ -19,8 +17,7 @@ fi coverage_file="${test_volume}/coverage.xml" -if [[ ! -f $coverage_file ]] -then +if [[ ! -f $coverage_file ]]; then echo "Coverage file wasn't found, were the tests run before?" exit 0 # exit this script but don't fail the tests for this. fi @@ -30,7 +27,7 @@ output_file="${test_volume}/${project}_coverage.xml" # In the test coverage report, all file paths are relative to each project # folder. We need to be relative to the repo's root directory. That's why we # append the project folder name to each file path in coverage.xml -sed "s/filename=\"/filename=\"$project\//g" "$coverage_file" > "$output_file" +sed "s/filename=\"/filename=\"$project\//g" "$coverage_file" >"$output_file" # codecov.sh is located at https://codecov.io/bash # we downloaded it for convenience diff --git a/scripts/prepare_image.sh b/scripts/prepare_image.sh index 67f92e4d1..dddf9132d 100755 --- a/scripts/prepare_image.sh +++ b/scripts/prepare_image.sh @@ -100,6 +100,7 @@ fi # We want to check if a test image has been built for this branch. If # it has we should use that rather than building it slowly. DOCKER_IMAGE="$DOCKERHUB_REPO/dr_$IMAGE" + # shellcheck disable=SC2086 if [ "$(docker_img_exists $IMAGE_NAME $branch_name)" ]; then docker pull "$IMAGE_NAME:$branch_name" @@ -111,20 +112,21 @@ else echo "" echo "Rebuilding the $DOCKER_IMAGE image." - attempts=0 + attempt=0 + attempts=3 finished=1 - while [ $finished != 0 ] && [ $attempts -lt 3 ]; do + while [ $finished != 0 ] && [ $attempt -lt $attempts ]; do if [ $attempts -gt 0 ]; then echo "Failed to build $DOCKER_IMAGE, trying again." fi - CURRENT_IMAGE="$DOCKER_IMAGE:$SYSTEM_VERSION" - LATEST_IMAGE="$DOCKER_IMAGE:latest" if test "$GITHUB_ACTIONS"; then # Docker needs repositories to be lowercase. CACHE_REPO="$(echo "ghrc.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]')" - LATEST_IMAGE="$CACHE_REPO/dr_$IMAGE" + CACHE_FROM_IMAGE="$CACHE_REPO/dr_$IMAGE" + else + CACHE_FROM_IMAGE=$DOCKER_IMAGE fi if test "$DOCKER_BUILDER"; then @@ -136,18 +138,19 @@ else --build-arg BUILDKIT_INLINE_CACHE=1 \ --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ - --cache-from "$LATEST_IMAGE" \ - --cache-from="$CURRENT_IMAGE" \ + --cache-from "$CACHE_FROM_IMAGE:$SYSTEM_VERSION" \ + --cache-from "$CACHE_FROM_IMAGE:latest" \ --file "$SERVICE/dockerfiles/Dockerfile.$IMAGE" \ --platform linux/amd64 \ --tag "$DOCKER_IMAGE" \ . + finished=$? - attempts=$((attempts + 1)) + attempt=$((attempt + 1)) done - if [ $finished != 0 ] && [ $attempts -ge 3 ]; then - echo "Could not build $DOCKER_IMAGE after three attempts." + if [ $finished -ne 0 ] && [ $attempt -ge $attempts ]; then + echo "Could not build $DOCKER_IMAGE after $attempt attempts." exit 1 fi fi diff --git a/scripts/update_my_docker_images.sh b/scripts/update_my_docker_images.sh index d64d48694..f3ab3aea6 100755 --- a/scripts/update_my_docker_images.sh +++ b/scripts/update_my_docker_images.sh @@ -109,7 +109,7 @@ echo "$SYSTEM_VERSION" >common/version # required by the workers and data_refinery_foreman images. ## Remove old common distributions if they exist. rm -f common/dist/* -(cd common && python3 setup.py sdist) # Run in a subshell. +(cd common && python3 setup.py sdist 1>/dev/null) # Run quietly in a subshell. ARCH="$(uname -m)" @@ -146,8 +146,8 @@ for DOCKER_IMAGE in $DOCKER_IMAGES; do --build-arg BUILDKIT_INLINE_CACHE=1 \ --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ - --cache-from="$IMAGE_NAME:$SYSTEM_VERSION" \ - --cache-from="$IMAGE_NAME:latest" \ + --cache-from "$IMAGE_NAME:$SYSTEM_VERSION" \ + --cache-from "$IMAGE_NAME:latest" \ --file "$DOCKER_FILE_PATH" \ --platform "linux/$SYSTEM_ARCH" \ --push \ From 1da019836048909cfa294fbc4effcbdb0f4906c3 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 12 May 2023 10:38:47 -0700 Subject: [PATCH 126/220] Update Github workflows config - Add common Dockerfile.base image job - Add dependencies: syntax check -> base image -> test jobs - Migrate to pre-commit actions - Add and title-case job/step names - Group and reorder jobs --- .github/workflows/config.yml | 415 ++++++++++++++++++++--------------- 1 file changed, 233 insertions(+), 182 deletions(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 94c906279..9a5b8a628 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -1,48 +1,47 @@ -name: test-and-deploy +name: refine.bio CI/CD on: push jobs: - syntax_test: - # ubuntu-latest is ubuntu 18.04 which has a really outdated version of shellcheck - runs-on: ubuntu-20.04 + # Code quality check jobs. + check_syntax: env: AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} + name: Check Syntax + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - + - uses: actions/setup-python@v3 - uses: hashicorp/setup-terraform@v2 with: terraform_version: 0.13.5 - - name: Install build & test dependencies - run: | - python -m pip install --upgrade pip - pip install pre-commit - - - name: Init terraform in the infrastructure folder for validation + - name: Init Terraform in the Infrastructure Folder for Validation run: cd infrastructure && TF_VAR_user=ci TF_VAR_stage=dev ./init_terraform.sh - - name: Init terraform in the AMI folder for validation + - name: Init Terraform in the AMI Folder for Validation run: cd ami && terraform init - - name: Run pre-commit hooks on all files - run: PATH="$PATH:$HOME/.local/bin" pre-commit run --all-files --show-diff-on-failure + - uses: pre-commit/action@v3.0.0 + # Test jobs. test_affy_agilent: - runs-on: ubuntu-latest env: - IMAGES: migrations affymetrix - DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: affymetrix migrations + name: Test Affymetrix and Agilent + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Free up space + - name: Free Up Space run: ./.github/scripts/cleanup_instance.sh - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io @@ -52,92 +51,128 @@ jobs: - name: Start Postgres run: ./scripts/run_postgres.sh - - name: Pause for initialization + - name: Pause for Initialization run: sleep 30 - - name: Install the database + - name: Install the Database run: ./scripts/install_db_docker.sh - - name: Update the models + - name: Update the Models run: | - sudo chmod -R a+wr common + sudo chmod -R a+rw common ./scripts/update_models.sh - - name: Run affymetrix tests + - name: Run Affymetrix Tests run: | sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t affymetrix - - name: Run agilent tests + - name: Run Agilent Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t agilent - # This runs downloader and NO_OP tests. - downloader_no_op_tests: - runs-on: ubuntu-latest + test_api: env: - # We have issues when downloading the no_op image, so for now let's just not pull it - # IMAGES: migrations api_local downloaders no_op foreman - IMAGES: migrations api_local downloaders foreman - DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: api_local migrations + name: Test API + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Login to Packages Container registry + - name: Free Up Space + run: ./.github/scripts/cleanup_instance.sh + + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} password: ${{ secrets.GITHUB_TOKEN }} - - name: Pull cached docker images + - name: Pull Cached Docker Images run: ./.github/scripts/pull_docker_images.sh - name: Start Postgres run: ./scripts/run_postgres.sh - - name: Pause for initialization + - name: Start Elasticsearch + run: ./scripts/run_es.sh + + - name: Pause for Initialization run: sleep 30 - - name: Install the database + - name: Install the Database run: ./scripts/install_db_docker.sh - - name: Update the models + - name: Update the Models run: | - sudo chmod -R a+wr common + sudo chmod -R a+rw common ./scripts/update_models.sh - - name: Run downloader tests - run: | - .github/scripts/filter_tests.sh -t downloaders + - name: Rebuild the Elasticsearch Index + run: ./scripts/rebuild_es_index.sh - - name: Run NO_OP tests + - name: Run API Tests run: | - sudo -E chown -R $USER:$USER workers/test_volume - .github/scripts/filter_tests.sh -t no_op + mkdir -p test_volume + sudo chmod -R a+rw test_volume + ./api/run_tests.sh + .github/scripts/upload_test_coverage.sh api - test_api: - runs-on: ubuntu-latest + - name: Push Built Docker Images + run: ./.github/scripts/push_docker_images.sh + + test_base: env: - IMAGES: migrations api_local - DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: base + name: Test Base + needs: + - check_syntax + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Free up space - run: ./.github/scripts/cleanup_instance.sh + - name: Login to Packages Container Registry + uses: docker/login-action@v2 + with: + registry: ghcr.io + username: ${{ github.actor }} + password: ${{ secrets.GITHUB_TOKEN }} + + - name: Pull Cached Docker Images + run: ./.github/scripts/pull_docker_images.sh + + - name: Build the Docker Image + run: ./scripts/prepare_image.sh -i base -s common + + - name: Push Built Docker Images + run: ./.github/scripts/push_docker_images.sh - - name: Login to Packages Container registry + test_common: + env: + CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: common_tests migrations + name: Test Common + needs: + - test_base + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} password: ${{ secrets.GITHUB_TOKEN }} - - name: Pull cached docker images + - name: Pull Cached Docker Images run: ./.github/scripts/pull_docker_images.sh - name: Start Postgres @@ -146,99 +181,98 @@ jobs: - name: Start Elasticsearch run: ./scripts/run_es.sh - - name: Pause for initialization + - name: Pause for Initialization run: sleep 30 - - name: Install the database + - name: Install the Database run: ./scripts/install_db_docker.sh - - name: Update the models + - name: Update the Models run: | - sudo chmod -R a+wr common + sudo chmod -R a+rw common ./scripts/update_models.sh - - name: Rebuild the Elasticsearch index + - name: Rebuild the Elasticsearch Index run: ./scripts/rebuild_es_index.sh - - name: Run API tests + - name: Run Common Tests run: | - mkdir -p test_volume - sudo chmod -R a+rw test_volume - ./api/run_tests.sh - .github/scripts/upload_test_coverage.sh api + mkdir -p workers/test_volume + sudo chmod -R a+rw workers/test_volume + ./common/run_tests.sh + .github/scripts/upload_test_coverage.sh common - - name: Push built docker images + - name: Push Built Docker Images run: ./.github/scripts/push_docker_images.sh - test_common: - runs-on: ubuntu-latest + test_downloaders_no_op: env: - IMAGES: migrations common_tests - DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: api_local downloaders foreman migrations no_op + name: Test Downloaders and No-Op + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} password: ${{ secrets.GITHUB_TOKEN }} - - name: Pull cached docker images + - name: Pull Cached Docker Images run: ./.github/scripts/pull_docker_images.sh - name: Start Postgres run: ./scripts/run_postgres.sh - - name: Start Elasticsearch - run: ./scripts/run_es.sh - - - name: Pause for initialization + - name: Pause for Initialization run: sleep 30 - - name: Install the database + - name: Install the Database run: ./scripts/install_db_docker.sh - - name: Update the models + - name: Update the Models run: | - sudo chmod -R a+wr common + sudo chmod -R a+rw common ./scripts/update_models.sh - - name: Rebuild the Elasticsearch index - run: ./scripts/rebuild_es_index.sh - - - name: Run Common tests + - name: Run Downloader Tests run: | - mkdir -p workers/test_volume - sudo chmod -R a+rw workers/test_volume - ./common/run_tests.sh - .github/scripts/upload_test_coverage.sh common + .github/scripts/filter_tests.sh -t downloaders - - name: Push built docker images - run: ./.github/scripts/push_docker_images.sh + - name: Run No-Op Tests + run: | + sudo -E chown -R $USER:$USER workers/test_volume + .github/scripts/filter_tests.sh -t no_op test_foreman: - runs-on: ubuntu-latest env: - IMAGES: migrations foreman - DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: migrations foreman + name: Test Foreman + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Free up space + - name: Free Up Space run: ./.github/scripts/cleanup_instance.sh - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} password: ${{ secrets.GITHUB_TOKEN }} - - name: Pull cached docker images + - name: Pull Cached Docker Images run: ./.github/scripts/pull_docker_images.sh - name: Start Postgres @@ -247,243 +281,235 @@ jobs: - name: Start Elasticsearch run: ./scripts/run_es.sh - - name: Pause for initialization + - name: Pause for Initialization run: sleep 30 - - name: Install the database + - name: Install the Database run: ./scripts/install_db_docker.sh - - name: Update the models + - name: Update the Models run: | - sudo chmod -R a+wr common + sudo chmod -R a+rw common ./scripts/update_models.sh - - name: Rebuild the Elasticsearch index + - name: Rebuild the Elasticsearch Index run: ./scripts/rebuild_es_index.sh - - name: Run Foreman tests + - name: Run Foreman Tests run: | mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume ./foreman/run_tests.sh --exclude-tag=end_to_end .github/scripts/upload_test_coverage.sh foreman - - name: Push built docker images + - name: Push Built Docker Images run: ./.github/scripts/push_docker_images.sh test_illumina: - runs-on: ubuntu-latest env: - IMAGES: migrations illumina - DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: illumina migrations + name: Test Illumina + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} password: ${{ secrets.GITHUB_TOKEN }} - - name: Pull cached docker images + - name: Pull Cached Docker Images run: ./.github/scripts/pull_docker_images.sh - name: Start Postgres run: ./scripts/run_postgres.sh - - name: Pause for initialization + - name: Pause for Initialization run: sleep 30 - - name: Install the database + - name: Install the Database run: ./scripts/install_db_docker.sh - - name: Update the models + - name: Update the Models run: | - sudo chmod -R a+wr common + sudo chmod -R a+rw common ./scripts/update_models.sh - - name: Run Illumina tests + - name: Run Illumina Tests run: | mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t illumina .github/scripts/upload_test_coverage.sh workers - - name: Push built docker images + - name: Push Built Docker Images run: ./.github/scripts/push_docker_images.sh test_salmon: - runs-on: ubuntu-latest env: - IMAGES: migrations salmon - DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: migrations salmon + name: Test Salmon + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} password: ${{ secrets.GITHUB_TOKEN }} - - name: Pull cached docker images + - name: Pull Cached Docker Images run: ./.github/scripts/pull_docker_images.sh - name: Start Postgres run: ./scripts/run_postgres.sh - - name: Pause for initialization + - name: Pause for Initialization run: sleep 30 - - name: Install the database + - name: Install the Database run: ./scripts/install_db_docker.sh - - name: Update the models + - name: Update the Models run: | - sudo chmod -R a+wr common + sudo chmod -R a+rw common ./scripts/update_models.sh - - name: Run Salmon tests + - name: Run Salmon Tests run: | sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t salmon .github/scripts/upload_test_coverage.sh workers - - name: Push built docker images + - name: Push Built Docker Images run: ./.github/scripts/push_docker_images.sh test_smasher: - runs-on: ubuntu-latest env: - IMAGES: migrations smasher - DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: migrations smasher + name: Test Smasher + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Free up space + - name: Free Up Space run: ./.github/scripts/cleanup_instance.sh - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} password: ${{ secrets.GITHUB_TOKEN }} - - name: Pull cached docker images + - name: Pull Cached Docker Images run: ./.github/scripts/pull_docker_images.sh - name: Start Postgres run: ./scripts/run_postgres.sh - - name: Pause for initialization + - name: Pause for Initialization run: sleep 30 - - name: Install the database + - name: Install the Database run: ./scripts/install_db_docker.sh - - name: Update the models + - name: Update the Models run: | - sudo chmod -R a+wr common + sudo chmod -R a+rw common ./scripts/update_models.sh - - name: Run smasher tests + - name: Run Smasher Tests run: | mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t smasher .github/scripts/upload_test_coverage.sh workers - - name: Run janitor tests + - name: Run Janitor Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t janitor .github/scripts/upload_test_coverage.sh workers - - name: Run compendia tests + - name: Run Compendia Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t compendia .github/scripts/upload_test_coverage.sh workers - - name: Run QN tests + - name: Run QN Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t qn .github/scripts/upload_test_coverage.sh workers - - name: Push built docker images + - name: Push Built Docker Images run: ./.github/scripts/push_docker_images.sh test_transcriptome: - runs-on: ubuntu-latest env: - IMAGES: migrations transcriptome - DOCKER_BUILDKIT: 1 CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DOCKER_BUILDKIT: 1 + IMAGES: migrations transcriptome + name: Test Transcriptome + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} password: ${{ secrets.GITHUB_TOKEN }} - - name: Pull cached docker images + - name: Pull Cached Docker Images run: ./.github/scripts/pull_docker_images.sh - name: Start Postgres run: ./scripts/run_postgres.sh - - name: Pause for initialization + - name: Pause for Initialization run: sleep 30 - - name: Install the database + - name: Install the Database run: ./scripts/install_db_docker.sh - - name: Update the models + - name: Update the Models run: | - sudo chmod -R a+wr common + sudo chmod -R a+rw common ./scripts/update_models.sh - - name: Run Transcriptome tests + - name: Run Transcriptome Tests run: | mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t transcriptome .github/scripts/upload_test_coverage.sh workers - - name: Push built docker images + - name: Push Built Docker Images run: ./.github/scripts/push_docker_images.sh - determine_branch: - # As far as I can tell, this is the only way to use the output of - # a script in a github conditional. - if: startsWith(github.ref, 'refs/tags/v') - runs-on: ubuntu-latest - outputs: - branch: ${{ steps.set_branch.outputs.branch }} - steps: - - uses: actions/checkout@v3 - with: - fetch-depth: 0 - - - id: set_branch - name: Set the $CI_TAG environment variable - run: | - source scripts/common.sh - echo "branch=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_OUTPUT - + # Deploy jobs. deploy: - if: startsWith(github.ref, 'refs/tags/v') && ! endsWith(github.ref, '-hotfix') - runs-on: ubuntu-latest - timeout-minutes: 1440 env: DEPLOY_IP_ADDRESS: ${{ secrets.DEPLOY_IP_ADDRESS }} DOCKER_ID: ${{ secrets.DOCKER_ID }} @@ -502,31 +528,35 @@ jobs: PROD_DJANGO_SECRET_KEY: ${{ secrets.PROD_DJANGO_SECRET_KEY }} PROD_RAVEN_DSN: ${{ secrets.PROD_RAVEN_DSN }} PROD_RAVEN_DSN_API: ${{ secrets.PROD_RAVEN_DSN_API }} + if: startsWith(github.ref, 'refs/tags/v') && ! endsWith(github.ref, '-hotfix') + name: Deploy needs: + - check_syntax - determine_branch - - downloader_no_op_tests - - syntax_test - test_affy_agilent - test_api + - test_base - test_common + - test_downloaders_no_op - test_foreman - test_illumina - test_salmon - test_smasher - test_transcriptome + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 with: fetch-depth: 0 - - name: Set the $MASTER_OR_DEV and $CI_TAG environment variables + - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables run: | source scripts/common.sh echo "MASTER_OR_DEV=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV - - name: Set staging specific environment variables + - name: Set Staging Specific Environment Variables if: ${{needs.determine_branch.outputs.branch == 'dev'}} run: | echo "DATABASE_PASSWORD=${STAGING_DATABASE_PASSWORD}" >> $GITHUB_ENV @@ -536,7 +566,7 @@ jobs: # Staging doesn't notify the engagementbot, so overwrite this to be blank. echo "ENGAGEMENTBOT_WEBHOOK=" >> $GITHUB_ENV - - name: Set prod specific environment variables + - name: Set Production Specific Environment Variables if: ${{needs.determine_branch.outputs.branch == 'master'}} run: | echo "DATABASE_PASSWORD=${PROD_DATABASE_PASSWORD}" >> $GITHUB_ENV @@ -548,13 +578,11 @@ jobs: - name: Deploy run: ./.github/scripts/remote_deploy.sh - - name: Cleanup deploy + - name: Cleanup Deploy run: ./.github/scripts/post_deploy_cleanup.sh - - hotfix_deploy: - if: startsWith(github.ref, 'refs/tags/v') && endsWith(github.ref, '-hotfix') - runs-on: ubuntu-latest timeout-minutes: 1440 + + deploy_hotfix: env: DEPLOY_IP_ADDRESS: ${{ secrets.DEPLOY_IP_ADDRESS }} DOCKER_ID: ${{ secrets.DOCKER_ID }} @@ -573,21 +601,24 @@ jobs: PROD_DJANGO_SECRET_KEY: ${{ secrets.PROD_DJANGO_SECRET_KEY }} PROD_RAVEN_DSN: ${{ secrets.PROD_RAVEN_DSN }} PROD_RAVEN_DSN_API: ${{ secrets.PROD_RAVEN_DSN_API }} + if: startsWith(github.ref, 'refs/tags/v') && endsWith(github.ref, '-hotfix') + name: Deploy Hotfix needs: - determine_branch + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 with: fetch-depth: 0 - - name: Set the $MASTER_OR_DEV and $CI_TAG environment variables + - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables run: | source scripts/common.sh echo "MASTER_OR_DEV=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV - - name: Set staging specific environment variables + - name: Set Staging Specific Environment Variables if: ${{needs.determine_branch.outputs.branch == 'dev'}} run: | echo "DATABASE_PASSWORD=${STAGING_DATABASE_PASSWORD}" >> $GITHUB_ENV @@ -597,7 +628,7 @@ jobs: # Staging doesn't notify the engagementbot, so overwrite this to be blank. echo "ENGAGEMENTBOT_WEBHOOK=" >> $GITHUB_ENV - - name: Set prod specific environment variables + - name: Set Production Specific Environment Variables if: ${{needs.determine_branch.outputs.branch == 'master'}} run: | echo "DATABASE_PASSWORD=${PROD_DATABASE_PASSWORD}" >> $GITHUB_ENV @@ -609,5 +640,25 @@ jobs: - name: Deploy run: ./.github/scripts/remote_deploy.sh - - name: Cleanup deploy + - name: Cleanup Deploy run: ./.github/scripts/post_deploy_cleanup.sh + timeout-minutes: 1440 + + determine_branch: + # As far as I can tell, this is the only way to use the output of + # a script in a github conditional. + if: startsWith(github.ref, 'refs/tags/v') + name: Determene Branch + runs-on: ubuntu-latest + outputs: + branch: ${{ steps.set_branch.outputs.branch }} + steps: + - uses: actions/checkout@v3 + with: + fetch-depth: 0 + + - id: set_branch + name: Set the $CI_TAG Environment Variable + run: | + source scripts/common.sh + echo "branch=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_OUTPUT From 8c705894c00d2f71f906c686e3d8ae4d8562c392 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 12 May 2023 10:58:48 -0700 Subject: [PATCH 127/220] Fix GHCR URL typo --- scripts/prepare_image.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/scripts/prepare_image.sh b/scripts/prepare_image.sh index e791ce589..81975da90 100755 --- a/scripts/prepare_image.sh +++ b/scripts/prepare_image.sh @@ -104,7 +104,7 @@ else if test "$GITHUB_ACTIONS"; then # docker needs repositories to be lowercase - CACHE_REPO="$(echo "ghrc.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]')" + CACHE_REPO="$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]')" CACHED_PACKAGE="$CACHE_REPO/dr_$image" CACHE="--build-arg BUILDKIT_INLINE_CACHE=1 --cache-from $CACHED_PACKAGE" fi From 05b00c5f76ab1cfce7fbb7048dfd26a2c315516d Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 17 May 2023 14:44:06 -0400 Subject: [PATCH 128/220] response changed, update cassettes --- ....sra.arrayexpress_alternate_accession.yaml | 4364 ++++----------- ...or.sra.metadata_is_gathered_correctly.yaml | 134 +- .../cassettes/surveyor.sra.srp_survey.yaml | 4824 +++++------------ .../surveyor.sra.survey_nonexistant.yaml | 6 +- .../surveyor.sra.survey_unmated_reads.yaml | 1890 ++----- 5 files changed, 3018 insertions(+), 8200 deletions(-) diff --git a/test_volume/cassettes/surveyor.sra.arrayexpress_alternate_accession.yaml b/test_volume/cassettes/surveyor.sra.arrayexpress_alternate_accession.yaml index 7301c90d8..601df41e1 100644 --- a/test_volume/cassettes/surveyor.sra.arrayexpress_alternate_accession.yaml +++ b/test_volume/cassettes/surveyor.sra.arrayexpress_alternate_accession.yaml @@ -9,7 +9,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -69,7 +69,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:40:58 GMT + - Wed, 17 May 2023 18:35:36 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -87,7 +87,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534072 response: @@ -128,7 +128,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:40:58 GMT + - Wed, 17 May 2023 18:35:36 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -146,7 +146,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552493 response: @@ -210,7 +210,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:40:59 GMT + - Wed, 17 May 2023 18:35:37 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -228,63 +228,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428787 response: body: string: "\n\n\n \n ERS2428787\n - \ SAMEA4608091\n + alias=\"E-MTAB-6681:G144_KO8_RNAseq_DMSO1\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428787\n SAMEA4608091\n \ E-MTAB-6681:G144_KO8_RNAseq_DMSO1\n \n \ G144_KO8_RNAseq_DMSO1\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552493\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534072\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #8\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 26844500\n - \ \n \n ENA-BASE-COUNT\n - \ 2027143150\n \n + \ \n individual\n clonal + line #8\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -294,7 +282,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:40:59 GMT + - Wed, 17 May 2023 18:35:38 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -312,7 +300,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -372,7 +360,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:00 GMT + - Wed, 17 May 2023 18:35:38 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -390,7 +378,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -426,7 +414,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:00 GMT + - Wed, 17 May 2023 18:35:39 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -434,68 +422,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534072&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrCMBAA0L1fEXDoVLm7pk3S3VkoTm7JNaVKMKGNiHAfr298JzpDc+y+vINc - 5pmGXoMhsW4gHIGsEKDtwHTgbojTgJN2d+Ex+oDWWeo1MRheYk/Ijldn/IJBRDSAXF/pq9qt1nK0 - quy5Zs5JPQ7lU8qfuKg176pu/8jhGbk2PwAAAP//AwCDIi4MjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:01 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95EE27101B2CD10000000000000001.m_1 - NCBI-SID: - - CC95EE27101B2CD1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95EE27101B2CD1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:01 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -506,29 +432,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534072&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534072\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/002/ERR2534072/ERR2534072.fastq.gz\t1342496804\t060aa7646a7caa6b7db9e9b5126efd99\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534072/G144_KO8_RNAseq_DMSO1.fastq.gz\t1397343408\t6f75b7add12efbe0413473419ed2f51b\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534072\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/002/ERR2534072/ERR2534072.fastq.gz\t1342496804\t060aa7646a7caa6b7db9e9b5126efd99\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534072/G144_KO8_RNAseq_DMSO1.fastq.gz\t1397343408\t6f75b7add12efbe0413473419ed2f51b\tftp.sra.ebi.ac.uk/vol1/err/ERR253/002/ERR2534072\t895216008\t2ecc0c89596bcf20c7c1f8e77ab3d9f5\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '483' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:02 GMT + - Wed, 17 May 2023 18:35:39 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -537,7 +461,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -548,7 +472,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534073 response: @@ -589,7 +513,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:03 GMT + - Wed, 17 May 2023 18:35:40 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -607,7 +531,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552494 response: @@ -671,7 +595,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:03 GMT + - Wed, 17 May 2023 18:35:40 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -689,63 +613,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428788 response: body: string: "\n\n\n \n ERS2428788\n - \ SAMEA4608092\n + alias=\"E-MTAB-6681:G144_KO8_RNAseq_DMSO2\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428788\n SAMEA4608092\n \ E-MTAB-6681:G144_KO8_RNAseq_DMSO2\n \n \ G144_KO8_RNAseq_DMSO2\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552494\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534073\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #8\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 23128792\n - \ \n \n ENA-BASE-COUNT\n - \ 1746472850\n \n + \ \n individual\n clonal + line #8\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -755,7 +667,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:04 GMT + - Wed, 17 May 2023 18:35:40 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -773,7 +685,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -833,7 +745,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:05 GMT + - Wed, 17 May 2023 18:35:41 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -851,7 +763,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -887,7 +799,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:05 GMT + - Wed, 17 May 2023 18:35:41 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -895,68 +807,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534073&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIuwrCMBQA0L1fEXDoVLl5p92dheLkdtOkVAkmJBER7sfrGc9JnGFoFcvb02Vd - hZYKrCRrwTlpjCQB3E1gJ5hvnC9aLlzdacao0ShUXntQlnMEMLg744N36AIRKQC6vtKXjUfvpY2s - 1NzzlhN7NIYp5U8MbM+V9eMf2T/j1ocfAAAA//8DAIRWqlaOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:06 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95EE67101B2D210000000000000001.m_1 - NCBI-SID: - - CC95EE67101B2D21_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95EE67101B2D21_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:06 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -967,29 +817,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534073&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534073\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/003/ERR2534073/ERR2534073.fastq.gz\t1156190981\td46334eb88f03b4c0713c7ed8abc231c\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534073/G144_KO8_RNAseq_DMSO2.fastq.gz\t1203618482\td2b9ae59efaa7491eac26723ce6022e7\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534073\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/003/ERR2534073/ERR2534073.fastq.gz\t1156190981\td46334eb88f03b4c0713c7ed8abc231c\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534073/G144_KO8_RNAseq_DMSO2.fastq.gz\t1203618482\td2b9ae59efaa7491eac26723ce6022e7\tftp.sra.ebi.ac.uk/vol1/err/ERR253/003/ERR2534073\t770883643\t616f7e4782c7ce9187068202610c359b\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '483' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:06 GMT + - Wed, 17 May 2023 18:35:42 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -998,7 +846,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -1009,7 +857,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534074 response: @@ -1050,7 +898,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:07 GMT + - Wed, 17 May 2023 18:35:42 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1068,7 +916,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552495 response: @@ -1132,7 +980,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:07 GMT + - Wed, 17 May 2023 18:35:43 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1150,63 +998,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428789 response: body: string: "\n\n\n \n ERS2428789\n - \ SAMEA4608093\n + alias=\"E-MTAB-6681:G144_KO9_RNAseq_DMSO1\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428789\n SAMEA4608093\n \ E-MTAB-6681:G144_KO9_RNAseq_DMSO1\n \n \ G144_KO9_RNAseq_DMSO1\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552495\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534074\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #9\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 22035122\n - \ \n \n ENA-BASE-COUNT\n - \ 1663855259\n \n + \ \n individual\n clonal + line #9\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -1216,7 +1052,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:08 GMT + - Wed, 17 May 2023 18:35:43 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1234,7 +1070,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -1294,7 +1130,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:09 GMT + - Wed, 17 May 2023 18:35:43 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1312,7 +1148,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -1348,7 +1184,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:10 GMT + - Wed, 17 May 2023 18:35:44 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1356,68 +1192,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534074&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrDIBAA0D1fIXTIlHJ6Gmv2zoXQqdtFDWmRGNRSCvfx7RvfSZ2hq4WO98LX - eVYGNVjNFtE6NBpYgbwMYAdwdyknoyd0Dx6DBIdKKiITCONiR3LaO4XkZbQrM2sAvu3pK/qttaP2 - 4ii5ZZ+TeFZBKeVPDGLNRbTtH3l5Rd+6HwAAAP//AwC73zj3jgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:10 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A41D9A101B2D610000000000000001.m_1 - NCBI-SID: - - C7A41D9A101B2D61_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A41D9A101B2D61_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:10 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -1428,29 +1202,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534074&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534074\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/004/ERR2534074/ERR2534074.fastq.gz\t1100830559\t895a107af87e643e80f1aa33dda08a18\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534074/G144_KO9_RNAseq_DMSO1.fastq.gz\t1145761230\ta75ea41c5c7c576caa5200cf820ee65c\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534074\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/004/ERR2534074/ERR2534074.fastq.gz\t1100830559\t895a107af87e643e80f1aa33dda08a18\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534074/G144_KO9_RNAseq_DMSO1.fastq.gz\t1145761230\ta75ea41c5c7c576caa5200cf820ee65c\tftp.sra.ebi.ac.uk/vol1/err/ERR253/004/ERR2534074\t733793520\t4f79fc0ea6323a394a2adb88fbc571b4\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '483' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:10 GMT + - Wed, 17 May 2023 18:35:44 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -1459,7 +1231,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -1470,7 +1242,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534075 response: @@ -1511,7 +1283,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:11 GMT + - Wed, 17 May 2023 18:35:45 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1529,7 +1301,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552496 response: @@ -1593,7 +1365,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:11 GMT + - Wed, 17 May 2023 18:35:45 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1611,63 +1383,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428790 response: body: string: "\n\n\n \n ERS2428790\n - \ SAMEA4608094\n + alias=\"E-MTAB-6681:G144_KO9_RNAseq_DMSO2\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428790\n SAMEA4608094\n \ E-MTAB-6681:G144_KO9_RNAseq_DMSO2\n \n \ G144_KO9_RNAseq_DMSO2\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552496\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534075\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #9\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 19495488\n - \ \n \n ENA-BASE-COUNT\n - \ 1471706561\n \n + \ \n individual\n clonal + line #9\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -1677,7 +1437,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:13 GMT + - Wed, 17 May 2023 18:35:45 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1695,7 +1455,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -1755,7 +1515,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:14 GMT + - Wed, 17 May 2023 18:35:46 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1773,7 +1533,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -1809,7 +1569,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:14 GMT + - Wed, 17 May 2023 18:35:46 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1817,68 +1577,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534075&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrDIBAA0D1fIXTIlHKeGk32zoXQqZsaJS1SRS2lcB/fvvGd8AxDq7a8HV22 - DZWQoBXNMwgtOUdC4GYCPcFy43xVahV4p9ma6DAGuWgTHDpvFWi9o1EOoghIRBKArq/0ZePRe2kj - KzX37HNij8ZsSvkTdhZzZf34R3bP4PvwAwAA//8DAOJq3zaOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:14 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A41DAB101B2DA10000000000000001.m_1 - NCBI-SID: - - C7A41DAB101B2DA1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A41DAB101B2DA1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:14 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -1889,29 +1587,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534075&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534075\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/005/ERR2534075/ERR2534075.fastq.gz\t988527951\td8909dd7d2ebe993b6a0136f1fdbbbdf\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534075/G144_KO9_RNAseq_DMSO2.fastq.gz\t1028859346\tac67f56d04d171f4848bd680f7bc0948\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534075\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/005/ERR2534075/ERR2534075.fastq.gz\t988527951\td8909dd7d2ebe993b6a0136f1fdbbbdf\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534075/G144_KO9_RNAseq_DMSO2.fastq.gz\t1028859346\tac67f56d04d171f4848bd680f7bc0948\tftp.sra.ebi.ac.uk/vol1/err/ERR253/005/ERR2534075\t660374092\t7a68cba094baf28e6b39d06d23e231e6\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '482' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:15 GMT + - Wed, 17 May 2023 18:35:47 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -1920,7 +1616,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -1931,7 +1627,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534076 response: @@ -1972,7 +1668,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:15 GMT + - Wed, 17 May 2023 18:35:47 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1990,7 +1686,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552497 response: @@ -2058,7 +1754,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:16 GMT + - Wed, 17 May 2023 18:35:48 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2076,63 +1772,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428791 response: body: string: "\n\n\n \n ERS2428791\n - \ SAMEA4608095\n + alias=\"E-MTAB-6681:G144_KO8_RNAseq_24h_1\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428791\n SAMEA4608095\n \ E-MTAB-6681:G144_KO8_RNAseq_24h_1\n \n \ G144_KO8_RNAseq_24h_1\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552497\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534076\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #8\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 25500197\n - \ \n \n ENA-BASE-COUNT\n - \ 1925621166\n \n + \ \n individual\n clonal + line #8\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -2142,7 +1826,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:16 GMT + - Wed, 17 May 2023 18:35:47 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2160,7 +1844,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -2220,7 +1904,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:17 GMT + - Wed, 17 May 2023 18:35:48 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2238,7 +1922,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -2274,7 +1958,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:19 GMT + - Wed, 17 May 2023 18:35:48 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2282,68 +1966,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534076&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIvQrCMBAA4L1PEXDoVLn8XJJ2dxaKk1uaS6gSTGkjItzD6zd+J3WG7tjD9l74 - Ms8KtQFn2aNFVB5GViD9AG6A8SblhDgZe2cXLfkcfDBZk9PREAVIlnSmMUFGZjYAfH2Vr+jX1raj - F9teW421iMchQin1k0jkuou2/qMuzxRb9wMAAP//AwChs8VGjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:19 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A41DBE101B2DF10000000000000001.m_1 - NCBI-SID: - - C7A41DBE101B2DF1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A41DBE101B2DF1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:19 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -2354,29 +1976,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534076&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534076\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/006/ERR2534076/ERR2534076.fastq.gz\t1282020356\t40598b820ef337261d26b6135301d8ad\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534076/G144_KO8_RNAseq_24h_1.fastq.gz\t1336023529\t87ef33d7d33e0eb591e1d11482e7f961\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534076\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/006/ERR2534076/ERR2534076.fastq.gz\t1282020356\t40598b820ef337261d26b6135301d8ad\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534076/G144_KO8_RNAseq_24h_1.fastq.gz\t1336023529\t87ef33d7d33e0eb591e1d11482e7f961\tftp.sra.ebi.ac.uk/vol1/err/ERR253/006/ERR2534076\t856552789\t3d7918ae8eda2f5fb3e47f742c3c692b\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '483' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:19 GMT + - Wed, 17 May 2023 18:35:49 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -2385,7 +2005,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -2396,7 +2016,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534077 response: @@ -2437,7 +2057,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:20 GMT + - Wed, 17 May 2023 18:35:49 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2455,7 +2075,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552498 response: @@ -2523,7 +2143,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:20 GMT + - Wed, 17 May 2023 18:35:50 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2541,63 +2161,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428792 response: body: string: "\n\n\n \n ERS2428792\n - \ SAMEA4608096\n + alias=\"E-MTAB-6681:G144_KO8_RNAseq_24h_2\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428792\n SAMEA4608096\n \ E-MTAB-6681:G144_KO8_RNAseq_24h_2\n \n \ G144_KO8_RNAseq_24h_2\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552498\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534077\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #8\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 22960406\n - \ \n \n ENA-BASE-COUNT\n - \ 1733737669\n \n + \ \n individual\n clonal + line #8\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -2607,7 +2215,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:21 GMT + - Wed, 17 May 2023 18:35:50 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2625,7 +2233,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -2685,7 +2293,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:25 GMT + - Wed, 17 May 2023 18:35:50 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2703,7 +2311,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -2739,7 +2347,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:26 GMT + - Wed, 17 May 2023 18:35:51 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2747,68 +2355,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534077&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIvQrCMBAA4L1PEXDoVLmkl1za3VkoTm7Nj1QJTUgjItzD6zd+J3WG7qhreTu+ - LIvSIwIRkyFtEcmwAmkHoAGmm5Sz1rPSd0YTRgOe0EYNYEkFOYWAiN5ImJxnZgTg656+ot9aK0cv - Ss0t+5zE8xBrSvkTg3jkKtr2j+xe0bfuBwAA//8DAHqUUgGOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:26 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A41DDD101B2E610000000000000001.m_1 - NCBI-SID: - - C7A41DDD101B2E61_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A41DDD101B2E61_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:26 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -2819,29 +2365,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534077&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534077\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/007/ERR2534077/ERR2534077.fastq.gz\t1150096005\t5a6030091948ae27ec5bc5a1ccb14001\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534077/G144_KO8_RNAseq_24h_2.fastq.gz\t1197876304\t4890986fa779d752b6c3fd4f08c6104f\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534077\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/007/ERR2534077/ERR2534077.fastq.gz\t1150096005\t5a6030091948ae27ec5bc5a1ccb14001\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534077/G144_KO8_RNAseq_24h_2.fastq.gz\t1197876304\t4890986fa779d752b6c3fd4f08c6104f\tftp.sra.ebi.ac.uk/vol1/err/ERR253/007/ERR2534077\t767584456\tdbccf1889a4cc651a06959ae3478d038\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '483' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:26 GMT + - Wed, 17 May 2023 18:35:52 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -2850,7 +2394,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -2861,7 +2405,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534078 response: @@ -2902,7 +2446,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:27 GMT + - Wed, 17 May 2023 18:35:52 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2920,7 +2464,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552499 response: @@ -2988,7 +2532,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:29 GMT + - Wed, 17 May 2023 18:35:52 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3006,63 +2550,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428793 response: body: string: "\n\n\n \n ERS2428793\n - \ SAMEA4608097\n + alias=\"E-MTAB-6681:G144_KO8_RNAseq_4h_1\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428793\n SAMEA4608097\n \ E-MTAB-6681:G144_KO8_RNAseq_4h_1\n \n \ G144_KO8_RNAseq_4h_1\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552499\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534078\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #8\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 30777620\n - \ \n \n ENA-BASE-COUNT\n - \ 2323852146\n \n + \ \n individual\n clonal + line #8\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -3072,7 +2604,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:29 GMT + - Wed, 17 May 2023 18:35:52 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3090,7 +2622,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -3150,7 +2682,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:30 GMT + - Wed, 17 May 2023 18:35:52 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3168,7 +2700,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -3204,7 +2736,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:30 GMT + - Wed, 17 May 2023 18:35:53 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3213,7 +2745,7 @@ interactions: code: 200 message: '' - request: - body: acc=ERR2534078&accept-proto=fasp&version=2.0 + body: null headers: Accept: - '*/*' @@ -3221,92 +2753,28 @@ interactions: - gzip, deflate Connection: - keep-alive - Content-Length: - - '44' User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi + - python-requests/2.30.0 + method: GET + uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534078&result=read_run response: body: - string: !!binary | - H4sIAAAAAAAAAwzKvQrCMBAA4L1PEXDoVLm7/DXdnYXi5JbUlCrBhCYiwj28XT++E52hq7svn8CX - eSYtFdiREUijliAVE+A4gB3A3RAnbSfSd16jds4btOCCOhqGaIgiGOcCKiRmVgB8faef6LfWSu1F - 2XPLS07iWYVPKX/jQ6x5F207IIdXXFr3BwAA//8DAACvVh+PAAAA + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534078\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/008/ERR2534078/ERR2534078.fastq.gz\t1536621662\taec2ace3d7e6c37ea2cc645fec91b508\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534078/G144_KO8_RNAseq_4h_1.fastq.gz\t1600009820\tf013cfe299039a31f0c60f89c94bddf5\tftp.sra.ebi.ac.uk/vol1/err/ERR253/008/ERR2534078\t1025153014\t6c80784e273565081f863cf58fae1fc9\n" headers: Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests + - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '483' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:31 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A41DF7101B2EB10000000000000001.m_1 - NCBI-SID: - - C7A41DF7101B2EB1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A41DF7101B2EB1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:31 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK -- request: - body: null - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - User-Agent: - - python-requests/2.25.1 - method: GET - uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534078&result=read_run - response: - body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534078\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/008/ERR2534078/ERR2534078.fastq.gz\t1536621662\taec2ace3d7e6c37ea2cc645fec91b508\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534078/G144_KO8_RNAseq_4h_1.fastq.gz\t1600009820\tf013cfe299039a31f0c60f89c94bddf5\t\t\t\n" - headers: - Cache-Control: - - no-cache, no-store, max-age=0, must-revalidate - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:32 GMT + - Wed, 17 May 2023 18:35:54 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -3315,7 +2783,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -3326,7 +2794,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534079 response: @@ -3367,7 +2835,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:32 GMT + - Wed, 17 May 2023 18:35:53 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3385,7 +2853,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552500 response: @@ -3453,7 +2921,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:32 GMT + - Wed, 17 May 2023 18:35:54 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3471,63 +2939,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428794 response: body: string: "\n\n\n \n ERS2428794\n - \ SAMEA4608098\n + alias=\"E-MTAB-6681:G144_KO8_RNAseq_4h_2\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428794\n SAMEA4608098\n \ E-MTAB-6681:G144_KO8_RNAseq_4h_2\n \n \ G144_KO8_RNAseq_4h_2\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552500\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534079\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #8\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 22511265\n - \ \n \n ENA-BASE-COUNT\n - \ 1699936076\n \n + \ \n individual\n clonal + line #8\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -3537,7 +2993,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:33 GMT + - Wed, 17 May 2023 18:35:55 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3555,7 +3011,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -3615,7 +3071,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:34 GMT + - Wed, 17 May 2023 18:35:55 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3633,7 +3089,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -3669,7 +3125,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:34 GMT + - Wed, 17 May 2023 18:35:55 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3677,68 +3133,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534079&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrCMBAA0L1fEXDoVLmcvVza3VkoTm5pklIlNKGNiHAfr298JzxDc+yuvGe5 - ThPSpQcehInI9gZBELTtgDsY7lqPxCPwQyxphx6BOBgbvKUFjdHIke3iHLKI9ABy29JXtWut5WhV - 2XPNPif1PJRLKX9iUEveVV3/kedX9LX5AQAA//8DAFfh71COAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:35 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95EF24101B2EF10000000000000001.m_1 - NCBI-SID: - - CC95EF24101B2EF1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95EF24101B2EF1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:35 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -3749,29 +3143,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534079&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534079\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/009/ERR2534079/ERR2534079.fastq.gz\t1132203170\t429470250bf1e5e7ab6fbb7fd7f1087f\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534079/G144_KO8_RNAseq_4h_2.fastq.gz\t1178873511\t24f54a4b765784df91b47d5ed39eb311\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534079\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/009/ERR2534079/ERR2534079.fastq.gz\t1132203170\t429470250bf1e5e7ab6fbb7fd7f1087f\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534079/G144_KO8_RNAseq_4h_2.fastq.gz\t1178873511\t24f54a4b765784df91b47d5ed39eb311\tftp.sra.ebi.ac.uk/vol1/err/ERR253/009/ERR2534079\t755584600\tdfc6e098febc3d3d5def75a8086fad94\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '482' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:35 GMT + - Wed, 17 May 2023 18:35:56 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -3780,7 +3172,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -3791,7 +3183,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534080 response: @@ -3832,7 +3224,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:35 GMT + - Wed, 17 May 2023 18:35:56 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3850,7 +3242,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552501 response: @@ -3918,7 +3310,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:36 GMT + - Wed, 17 May 2023 18:35:57 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3936,63 +3328,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428795 response: body: string: "\n\n\n \n ERS2428795\n - \ SAMEA4608099\n + alias=\"E-MTAB-6681:G144_KO9_RNAseq_24h_1\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428795\n SAMEA4608099\n \ E-MTAB-6681:G144_KO9_RNAseq_24h_1\n \n \ G144_KO9_RNAseq_24h_1\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552501\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534080\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #9\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 26709956\n - \ \n \n ENA-BASE-COUNT\n - \ 2016956425\n \n + \ \n individual\n clonal + line #9\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -4002,7 +3382,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:37 GMT + - Wed, 17 May 2023 18:35:57 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4020,7 +3400,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -4080,7 +3460,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:38 GMT + - Wed, 17 May 2023 18:35:58 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4098,7 +3478,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -4134,7 +3514,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:39 GMT + - Wed, 17 May 2023 18:35:57 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4142,68 +3522,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534080&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIvQrCMBAA4L1PEXDoVLlc89vdWShObr2YUiU0IY2IcA+v3/id8AzdUZfyJr7M - M+pRgQN2zqNHoywjSDeAHcDfpJy0nfR458Wq1YG3qzY+hjEESVaSISSnTCBkZgXA1z19Rb+1Vo5e - lJpbDjmJ5yGWlPInPsSaq2jbPzK9YmjdDwAA//8DAD+CjnCOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:39 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95EF3E101B2F310000000000000001.m_1 - NCBI-SID: - - CC95EF3E101B2F31_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95EF3E101B2F31_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:39 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -4214,29 +3532,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534080&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534080\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/000/ERR2534080/ERR2534080.fastq.gz\t1333847546\t831e6a1e98dca3e530e1409e73bac1ef\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534080/G144_KO9_RNAseq_24h_1.fastq.gz\t1388655605\t1cafe0e6f1cb09d106bd723397442c86\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534080\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/000/ERR2534080/ERR2534080.fastq.gz\t1333847546\t831e6a1e98dca3e530e1409e73bac1ef\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534080/G144_KO9_RNAseq_24h_1.fastq.gz\t1388655605\t1cafe0e6f1cb09d106bd723397442c86\tftp.sra.ebi.ac.uk/vol1/err/ERR253/000/ERR2534080\t889292627\tc6d6a22ba70b0c08eb9d64cb2584b472\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '483' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:40 GMT + - Wed, 17 May 2023 18:35:59 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -4245,7 +3561,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -4256,7 +3572,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534081 response: @@ -4297,7 +3613,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:40 GMT + - Wed, 17 May 2023 18:35:59 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4315,7 +3631,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552502 response: @@ -4383,7 +3699,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:40 GMT + - Wed, 17 May 2023 18:35:58 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4401,63 +3717,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428796 response: body: string: "\n\n\n \n ERS2428796\n - \ SAMEA4608100\n + alias=\"E-MTAB-6681:G144_KO9_RNAseq_24h_2\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428796\n SAMEA4608100\n \ E-MTAB-6681:G144_KO9_RNAseq_24h_2\n \n \ G144_KO9_RNAseq_24h_2\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552502\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534081\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #9\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 28684129\n - \ \n \n ENA-BASE-COUNT\n - \ 2165709760\n \n + \ \n individual\n clonal + line #9\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -4467,7 +3771,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:41 GMT + - Wed, 17 May 2023 18:36:00 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4485,7 +3789,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -4545,7 +3849,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:41 GMT + - Wed, 17 May 2023 18:36:00 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4563,7 +3867,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -4599,7 +3903,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:42 GMT + - Wed, 17 May 2023 18:36:00 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4607,68 +3911,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534081&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzLvQrDIBRA4T1PIXTIlHL91+ydC6FTN7VKWqSKWkrhPnyzno9zYmeYenP14/Gy - bUxyAYaiVYpJEJojA2oW0AvYG6WrtCvw+8E+OecNZ1yD9Z6rmICqEI89UsMQUQDg9Z1/ZN7HqH0m - tZVRQsnk2YnLuXzjg6TSyNiPUPwrhjH9AQAA//8DAAkU/ZeOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:43 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A41E75101B2F710000000000000001.m_1 - NCBI-SID: - - C7A41E75101B2F71_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A41E75101B2F71_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:43 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -4679,29 +3921,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534081&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534081\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/001/ERR2534081/ERR2534081.fastq.gz\t1446750089\t788fc2528be1fba017c3e3256d283a6b\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534081/G144_KO9_RNAseq_24h_2.fastq.gz\t1505999963\t7d8548778e311bb0dcc7eee2583dcedd\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534081\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/001/ERR2534081/ERR2534081.fastq.gz\t1446750089\t788fc2528be1fba017c3e3256d283a6b\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534081/G144_KO9_RNAseq_24h_2.fastq.gz\t1505999963\t7d8548778e311bb0dcc7eee2583dcedd\tftp.sra.ebi.ac.uk/vol1/err/ERR253/001/ERR2534081\t966250453\tf1d1b0d4d2bc133dfb5df3d4b11f0ea3\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '483' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:44 GMT + - Wed, 17 May 2023 18:36:01 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -4710,7 +3950,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -4721,7 +3961,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534082 response: @@ -4762,7 +4002,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:43 GMT + - Wed, 17 May 2023 18:36:01 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4780,7 +4020,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552503 response: @@ -4848,7 +4088,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:44 GMT + - Wed, 17 May 2023 18:36:02 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4866,63 +4106,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428797 response: body: string: "\n\n\n \n ERS2428797\n - \ SAMEA4608101\n + alias=\"E-MTAB-6681:G144_KO9_RNAseq_4h_1\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428797\n SAMEA4608101\n \ E-MTAB-6681:G144_KO9_RNAseq_4h_1\n \n \ G144_KO9_RNAseq_4h_1\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552503\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534082\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #9\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 36759653\n - \ \n \n ENA-BASE-COUNT\n - \ 2776723098\n \n + \ \n individual\n clonal + line #9\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -4932,7 +4160,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:44 GMT + - Wed, 17 May 2023 18:36:01 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4950,7 +4178,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -5010,7 +4238,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:45 GMT + - Wed, 17 May 2023 18:36:02 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5028,7 +4256,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -5064,7 +4292,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:46 GMT + - Wed, 17 May 2023 18:36:03 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5072,68 +4300,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534082&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQ6CMBQF0J2vaOLAhLnvtZXC7mxCnNxoKUHTUAI1xuR9vJ7xnPiM6tjH7e3l - OgxstYFjIdYgy9QaYZBr0Dbo7kS9dT3rh0xTB7pQCC54F52BZ+MjQc+sCbAiYgC5remr6qWU7ajV - tueSQ07qeagxpfyJk5rzrsryj+xfMZTqBwAA//8DAEXu4WyPAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:47 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A41EA2101B2FB10000000000000001.m_1 - NCBI-SID: - - C7A41EA2101B2FB1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A41EA2101B2FB1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:47 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -5144,29 +4310,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534082&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534082\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/002/ERR2534082/ERR2534082.fastq.gz\t1810089565\t7b93d8c5e3baaead488b919819d1f8b7\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534082/G144_KO9_RNAseq_4h_1.fastq.gz\t1914915905\t0050389f2fbb6b230a0adf29580c38df\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534082\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/002/ERR2534082/ERR2534082.fastq.gz\t1810089565\t7b93d8c5e3baaead488b919819d1f8b7\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534082/G144_KO9_RNAseq_4h_1.fastq.gz\t1914915905\t0050389f2fbb6b230a0adf29580c38df\tftp.sra.ebi.ac.uk/vol1/err/ERR253/002/ERR2534082\t1230152154\tdae4a49ebab73aa65c79030b43c2e931\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '483' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:48 GMT + - Wed, 17 May 2023 18:36:03 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -5175,7 +4339,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -5186,7 +4350,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534083 response: @@ -5227,7 +4391,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:49 GMT + - Wed, 17 May 2023 18:36:03 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5245,7 +4409,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552504 response: @@ -5313,7 +4477,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:49 GMT + - Wed, 17 May 2023 18:36:04 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5331,63 +4495,51 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428798 response: body: string: "\n\n\n \n ERS2428798\n - \ SAMEA4608102\n + alias=\"E-MTAB-6681:G144_KO9_RNAseq_4h_2\" center_name=\"BRIC - Biotech Research + and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n + \ ERS2428798\n SAMEA4608102\n \ E-MTAB-6681:G144_KO9_RNAseq_4h_2\n \n \ G144_KO9_RNAseq_4h_2\n \n 9606\n \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552504\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534083\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n - \ \n genotype\n CRISPR/Cas9 - mediated knockout of CIC\n \n \n - \ individual\n clonal line #9\n - \ \n \n ENA-CHECKLIST\n - \ ERC000011\n \n \n - \ ENA-SPOT-COUNT\n 28325395\n - \ \n \n ENA-BASE-COUNT\n - \ 2139937955\n \n + \ \n individual\n clonal + line #9\n \n \n + \ organism\n Homo sapiens\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n cell type\n glial + cell\n \n \n + \ common name\n human\n + \ \n \n organism + part\n brain\n \n + \ \n cell line\n G144\n + \ \n \n genotype\n + \ CRISPR/Cas9 mediated knockout of CIC\n \n \ \n ENA-FIRST-PUBLIC\n \ 2018-05-30\n \n \ \n ENA-LAST-UPDATE\n @@ -5397,7 +4549,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:50 GMT + - Wed, 17 May 2023 18:36:04 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5415,7 +4567,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -5475,7 +4627,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:51 GMT + - Wed, 17 May 2023 18:36:04 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5493,7 +4645,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -5529,7 +4681,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:51 GMT + - Wed, 17 May 2023 18:36:05 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5537,68 +4689,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534083&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQqDMBAA0N2vCHRwspx3F3O6dy5Ip25GI7aERkxKKdzHt298JzxDlY9pf3u9 - jCNaYhDS3pLjjp0oQisNuAb6W4sDwEDdXTtyKEKIC1MvsthAtEJoCW1gdl5VGUCvr/g19VbKnmuz - H6mkOUXzyGaKMX3CYtZ0mLL9I/lnmEv1AwAA//8DAGfSJeKOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:52 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A41EC6101B30010000000000000001.m_1 - NCBI-SID: - - C7A41EC6101B3001_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A41EC6101B3001_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:52 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -5609,29 +4699,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534083&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534083\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/003/ERR2534083/ERR2534083.fastq.gz\t1364338778\t5d9c21fcdbf30929dd54fe64a2858096\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534083/G144_KO9_RNAseq_4h_2.fastq.gz\t1476580896\tb175da8f8c4548f974ca380386daa3b9\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534083\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/003/ERR2534083/ERR2534083.fastq.gz\t1364338778\t5d9c21fcdbf30929dd54fe64a2858096\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534083/G144_KO9_RNAseq_4h_2.fastq.gz\t1476580896\tb175da8f8c4548f974ca380386daa3b9\tftp.sra.ebi.ac.uk/vol1/err/ERR253/003/ERR2534083\t953746458\ta8d792feb447419a62dc2ad2388904f9\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '482' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:52 GMT + - Wed, 17 May 2023 18:36:06 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -5640,7 +4728,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -5651,7 +4739,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534084 response: @@ -5693,7 +4781,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:53 GMT + - Wed, 17 May 2023 18:36:05 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5711,7 +4799,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552505 response: @@ -5775,7 +4863,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:53 GMT + - Wed, 17 May 2023 18:36:07 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5793,14 +4881,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428799 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_Parental_RNAseq_DMSO1\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428799\n SAMEA4608103\n \ E-MTAB-6681:G144_Parental_RNAseq_DMSO1\n \n @@ -5808,57 +4896,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552505\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534084\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 1\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 1\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 26499768\n \n \n - \ ENA-BASE-COUNT\n 2000152520\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:54 GMT + - Wed, 17 May 2023 18:36:07 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5876,7 +4952,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -5936,7 +5012,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:54 GMT + - Wed, 17 May 2023 18:36:07 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5954,7 +5030,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -5990,7 +5066,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:55 GMT + - Wed, 17 May 2023 18:36:07 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5998,68 +5074,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534084&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIvQrCMBAA4L1PEXDoVLlcc/np7iwUJ7cmbagSTGgiItzD6zd+JzxDV4+lvD1f - 5hlpVGAVW9RKAjrNCNIOYAZwNyknchOZOxOOgdDFqAKsligY2LxxOjqPZIJlZgXA11f6in5vrdRe - lCO3HHISjyqWlPJnW0XMh2j7P7J/bqF1PwAAAP//AwCTsKqLjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:41:56 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95EFB4101B30410000000000000001.m_1 - NCBI-SID: - - CC95EFB4101B3041_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95EFB4101B3041_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:41:56 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -6070,29 +5084,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534084&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534084\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/004/ERR2534084/ERR2534084.fastq.gz\t1246412999\t66ea10048085214a1d6a2164fad08440\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534084/G144_Parental_RNAseq_DMSO1.fastq.gz\t1298135488\ta174bf37dd39e1848734eebd87555d64\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534084\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/004/ERR2534084/ERR2534084.fastq.gz\t1246412999\t66ea10048085214a1d6a2164fad08440\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534084/G144_Parental_RNAseq_DMSO1.fastq.gz\t1298135488\ta174bf37dd39e1848734eebd87555d64\tftp.sra.ebi.ac.uk/vol1/err/ERR253/004/ERR2534084\t826410276\t07fa8afa15db6810690ce69acd63ec31\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '488' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:41:57 GMT + - Wed, 17 May 2023 18:36:08 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -6101,7 +5113,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -6112,7 +5124,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534085 response: @@ -6154,7 +5166,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:57 GMT + - Wed, 17 May 2023 18:36:08 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6172,7 +5184,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552506 response: @@ -6236,7 +5248,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:58 GMT + - Wed, 17 May 2023 18:36:08 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6254,14 +5266,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428800 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_Parental_RNAseq_DMSO2\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428800\n SAMEA4608104\n \ E-MTAB-6681:G144_Parental_RNAseq_DMSO2\n \n @@ -6269,57 +5281,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552506\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534085\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 1\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 1\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 21013213\n \n \n - \ ENA-BASE-COUNT\n 1586164243\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:41:59 GMT + - Wed, 17 May 2023 18:36:09 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6337,7 +5337,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -6397,7 +5397,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:00 GMT + - Wed, 17 May 2023 18:36:10 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6415,7 +5415,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -6451,7 +5451,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:00 GMT + - Wed, 17 May 2023 18:36:10 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6459,68 +5459,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534085&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQ6CMBQF0J2vaOLAhLl9bekru7MJcXIDLEHTUAI1xuR9vJ7xnOiM6tiH7T3K - pe/JGQt20jpjGGxICJob+AbhpqmD7uDvElsbbPSYPIeA0bchRBA7PZOfrWYRsYBc1/RV9VLKdtRq - 23PJU07qeaghpfyJDzXnXZXlH3l8xalUPwAAAP//AwCBHJBrjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:00 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95EFE5101B30810000000000000001.m_1 - NCBI-SID: - - CC95EFE5101B3081_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95EFE5101B3081_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:00 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -6531,29 +5469,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534085&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534085\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/005/ERR2534085/ERR2534085.fastq.gz\t986001790\t4921d2019bec9d2eb3746caca23307b2\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534085/G144_Parental_RNAseq_DMSO2.fastq.gz\t1027314622\t20fc8816e51bd3da97def0ead2a60b0b\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534085\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/005/ERR2534085/ERR2534085.fastq.gz\t986001790\t4921d2019bec9d2eb3746caca23307b2\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534085/G144_Parental_RNAseq_DMSO2.fastq.gz\t1027314622\t20fc8816e51bd3da97def0ead2a60b0b\tftp.sra.ebi.ac.uk/vol1/err/ERR253/005/ERR2534085\t653380812\t3bd5c5cdab98d0c41477923cb8135c14\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '487' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:01 GMT + - Wed, 17 May 2023 18:36:11 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -6562,7 +5498,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -6573,7 +5509,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534086 response: @@ -6615,7 +5551,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:01 GMT + - Wed, 17 May 2023 18:36:11 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6633,7 +5569,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552507 response: @@ -6697,7 +5633,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:02 GMT + - Wed, 17 May 2023 18:36:11 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6715,14 +5651,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428801 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_Parental_RNAseq_DMSO3\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428801\n SAMEA4608105\n \ E-MTAB-6681:G144_Parental_RNAseq_DMSO3\n \n @@ -6730,57 +5666,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552507\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534086\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 1\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 1\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 19622120\n \n \n - \ ENA-BASE-COUNT\n 1481141759\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:02 GMT + - Wed, 17 May 2023 18:36:12 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6798,7 +5722,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -6858,7 +5782,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:03 GMT + - Wed, 17 May 2023 18:36:12 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6876,7 +5800,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -6912,7 +5836,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:04 GMT + - Wed, 17 May 2023 18:36:12 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6920,68 +5844,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534086&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzKuwrCMBQA0L1fEXDoVLk33jza3VkoTm55SZXQhDYiwv14ux7OSZ6h2zdXP56v - 8yzVhcBq1hKBrNLIEtAOYAYY7ygnwIn0g8liNARxBHRJkUrJHNmFGFwIXidmJgC+rfkn+qW1uvei - bqWVULJ47cLlXL4pimfZRFsOKP6dQuv+AAAA//8DAGKPr42OAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:04 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A41F5B101B30C10000000000000001.m_1 - NCBI-SID: - - C7A41F5B101B30C1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A41F5B101B30C1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:04 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -6992,29 +5854,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534086&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534086\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/006/ERR2534086/ERR2534086.fastq.gz\t935756969\tf60033d4f40591a93092b83ad0a487f1\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534086/G144_Parental_RNAseq_DMSO3.fastq.gz\t974487429\t7d15b05abbea7e4f253dbe1311c983ee\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534086\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/006/ERR2534086/ERR2534086.fastq.gz\t935756969\tf60033d4f40591a93092b83ad0a487f1\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534086/G144_Parental_RNAseq_DMSO3.fastq.gz\t974487429\t7d15b05abbea7e4f253dbe1311c983ee\tftp.sra.ebi.ac.uk/vol1/err/ERR253/006/ERR2534086\t621048541\te8ab1e766393dcc1d82eb263b85dd56b\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '486' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:05 GMT + - Wed, 17 May 2023 18:36:13 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -7023,7 +5883,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -7034,7 +5894,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534087 response: @@ -7076,7 +5936,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:06 GMT + - Wed, 17 May 2023 18:36:14 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7094,7 +5954,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552508 response: @@ -7158,7 +6018,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:06 GMT + - Wed, 17 May 2023 18:36:13 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7176,14 +6036,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428802 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_WTclone_RNAseq_DMSO1\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428802\n SAMEA4608106\n \ E-MTAB-6681:G144_WTclone_RNAseq_DMSO1\n \n @@ -7191,57 +6051,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552508\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534087\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 2\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 2\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 24489613\n \n \n - \ ENA-BASE-COUNT\n 1849283386\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:07 GMT + - Wed, 17 May 2023 18:36:15 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7259,7 +6107,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -7319,7 +6167,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:08 GMT + - Wed, 17 May 2023 18:36:15 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7337,7 +6185,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -7373,7 +6221,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:08 GMT + - Wed, 17 May 2023 18:36:15 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7381,68 +6229,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534087&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrCMBAA0L1fEXDoVLm7pOm1u7NQnNySNKVKMCWNiHAfr298JzpDcxS3v71c - 5pl6bYAHYTRsrR5JCJA7GDoYb0gT4AT2LuDQc1iQHJKPMQJrz9BrWi0OwUcRMQByfaWvarda96NV - e8k1h5zU41AupfyJi1pzUXX7R/bPGGrzAwAA//8DAHWr/8SOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:08 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42023101B31110000000000000001.m_1 - NCBI-SID: - - C7A42023101B3111_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42023101B3111_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:09 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -7453,29 +6239,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534087&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534087\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/007/ERR2534087/ERR2534087.fastq.gz\t1222071540\t70a8511739490d8e3694c82dabf027d1\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534087/G144_WTclone_RNAseq_DMSO1.fastq.gz\t1272114070\t836d2e5186e468db4640ac7731e36f59\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534087\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/007/ERR2534087/ERR2534087.fastq.gz\t1222071540\t70a8511739490d8e3694c82dabf027d1\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534087/G144_WTclone_RNAseq_DMSO1.fastq.gz\t1272114070\t836d2e5186e468db4640ac7731e36f59\tftp.sra.ebi.ac.uk/vol1/err/ERR253/007/ERR2534087\t814866372\t5074b01b1cf6f1fc02bcfd476e074d8b\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '487' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:09 GMT + - Wed, 17 May 2023 18:36:16 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -7484,7 +6268,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -7495,7 +6279,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534088 response: @@ -7537,7 +6321,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:10 GMT + - Wed, 17 May 2023 18:36:16 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7555,7 +6339,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552509 response: @@ -7619,7 +6403,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:10 GMT + - Wed, 17 May 2023 18:36:16 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7637,14 +6421,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428803 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_WTclone_RNAseq_DMSO2\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428803\n SAMEA4608107\n \ E-MTAB-6681:G144_WTclone_RNAseq_DMSO2\n \n @@ -7652,57 +6436,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552509\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534088\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 2\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 2\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 21027363\n \n \n - \ ENA-BASE-COUNT\n 1587910747\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:11 GMT + - Wed, 17 May 2023 18:36:17 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7720,7 +6492,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -7780,7 +6552,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:12 GMT + - Wed, 17 May 2023 18:36:17 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7798,7 +6570,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -7834,7 +6606,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:12 GMT + - Wed, 17 May 2023 18:36:17 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7842,68 +6614,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534088&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzLsQqDMBAG4L1PEejgZPm9GO/i3rkgnbppVGwJjWhKKdzD1/WD70wXnPatXz+D - XruOnK0hogzyIhAooZISXMLfK2phW1QPZXZTI5ZDTTz6ETw4Dn4Wcg0f36pqDejtHX+mWHJe98Ks - W8oppGieu+ljTN9pNHPaTF4OSMNrCvn0BwAA//8DAGsMO5SOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:13 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F187101B31510000000000000001.m_1 - NCBI-SID: - - CC95F187101B3151_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F187101B3151_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:13 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -7914,29 +6624,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534088&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534088\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/008/ERR2534088/ERR2534088.fastq.gz\t1054521498\tab57f3177e3796fa02fa9c921ad187d2\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534088/G144_WTclone_RNAseq_DMSO2.fastq.gz\t1099839401\tc5d2613fe09ae1bbc2ace0892b8ce3ec\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534088\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/008/ERR2534088/ERR2534088.fastq.gz\t1054521498\tab57f3177e3796fa02fa9c921ad187d2\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534088/G144_WTclone_RNAseq_DMSO2.fastq.gz\t1099839401\tc5d2613fe09ae1bbc2ace0892b8ce3ec\tftp.sra.ebi.ac.uk/vol1/err/ERR253/008/ERR2534088\t702988060\t5a27cc3525de29872a1d83a10269e7bd\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '487' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:13 GMT + - Wed, 17 May 2023 18:36:18 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -7945,7 +6653,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -7956,7 +6664,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534089 response: @@ -7998,7 +6706,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:14 GMT + - Wed, 17 May 2023 18:36:18 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8016,7 +6724,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552510 response: @@ -8084,7 +6792,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:14 GMT + - Wed, 17 May 2023 18:36:19 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8102,14 +6810,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428804 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_Parental_RNAseq_24h_1\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428804\n SAMEA4608108\n \ E-MTAB-6681:G144_Parental_RNAseq_24h_1\n \n @@ -8117,57 +6825,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552510\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534089\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 1\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 1\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 19941600\n \n \n - \ ENA-BASE-COUNT\n 1505399955\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:15 GMT + - Wed, 17 May 2023 18:36:20 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8185,7 +6881,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -8245,7 +6941,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:16 GMT + - Wed, 17 May 2023 18:36:19 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8263,7 +6959,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -8299,7 +6995,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:16 GMT + - Wed, 17 May 2023 18:36:20 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8307,68 +7003,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534089&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrCMBAA0L1fEXDoVLleL5e0u7NQnNySNKVKMKWJiHAfr298JzxDUw63v71c - 5hn1QGBHYQTSPRoWhN52YDoYbz1OgBPZuwRjV2D2gZhwHRnJD5H9YjBaHZ0TEQKQ6yt9VbvVupdW - 7UeuOeSkHkW5lPInLmrNh6rbP7J/xlCbHwAAAP//AwB3pIyPjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:17 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F218101B31910000000000000001.m_1 - NCBI-SID: - - CC95F218101B3191_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F218101B3191_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:17 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -8379,29 +7013,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534089&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534089\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/009/ERR2534089/ERR2534089.fastq.gz\t936697735\t61cddd1766f5e4757fefd4a99865d3e0\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534089/G144_Parental_RNAseq_24h_1.fastq.gz\t975986547\ta1cd512c64c06f21259ee1c41beb2a5a\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534089\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/009/ERR2534089/ERR2534089.fastq.gz\t936697735\t61cddd1766f5e4757fefd4a99865d3e0\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534089/G144_Parental_RNAseq_24h_1.fastq.gz\t975986547\ta1cd512c64c06f21259ee1c41beb2a5a\tftp.sra.ebi.ac.uk/vol1/err/ERR253/009/ERR2534089\t620451256\tf566db3b1af03f9261b549a2f85ca46c\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '486' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:18 GMT + - Wed, 17 May 2023 18:36:21 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -8410,7 +7042,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -8421,7 +7053,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534090 response: @@ -8463,7 +7095,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:18 GMT + - Wed, 17 May 2023 18:36:20 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8481,7 +7113,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552511 response: @@ -8549,7 +7181,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:18 GMT + - Wed, 17 May 2023 18:36:21 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8567,14 +7199,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428805 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_Parental_RNAseq_24h_2\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428805\n SAMEA4608109\n \ E-MTAB-6681:G144_Parental_RNAseq_24h_2\n \n @@ -8582,57 +7214,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552511\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534090\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 1\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 1\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 22598916\n \n \n - \ ENA-BASE-COUNT\n 1705763883\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:19 GMT + - Wed, 17 May 2023 18:36:22 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8650,7 +7270,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -8710,7 +7330,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:21 GMT + - Wed, 17 May 2023 18:36:22 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8728,7 +7348,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -8764,7 +7384,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:21 GMT + - Wed, 17 May 2023 18:36:23 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8772,68 +7392,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534090&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIvQrCMBAA4L1PEXDoVLlc/i7dnYXi5NY2KVWCCWlEhHt4/cbvhGfojjqX98KX - aUKjNHhgB4o8gnaMIGkAN4C/SRwBR2XvbDyRXkluaIx1UgeM3lhScdkCzQ6YWQPw9ZW+ot9bK0cv - Ss0trzmJxyHmlPInBrHlKtr+j7w849q6HwAAAP//AwAtt9b2jgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:21 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42111101B31D10000000000000001.m_1 - NCBI-SID: - - C7A42111101B31D1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42111101B31D1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:21 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -8844,29 +7402,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534090&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534090\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/000/ERR2534090/ERR2534090.fastq.gz\t1061721210\tb6f84a04edb37b59613dc8d59e9647ce\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534090/G144_Parental_RNAseq_24h_2.fastq.gz\t1106158490\t50a58f0d2237a1bd2453da5df48228a8\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534090\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/000/ERR2534090/ERR2534090.fastq.gz\t1061721210\tb6f84a04edb37b59613dc8d59e9647ce\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534090/G144_Parental_RNAseq_24h_2.fastq.gz\t1106158490\t50a58f0d2237a1bd2453da5df48228a8\tftp.sra.ebi.ac.uk/vol1/err/ERR253/000/ERR2534090\t703892027\t530679b65da50bc03b2ea62c2e6e91d0\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '488' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:27 GMT + - Wed, 17 May 2023 18:36:23 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -8875,7 +7431,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -8886,7 +7442,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534091 response: @@ -8928,7 +7484,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:27 GMT + - Wed, 17 May 2023 18:36:24 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8946,7 +7502,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552512 response: @@ -9014,7 +7570,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:28 GMT + - Wed, 17 May 2023 18:36:24 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9032,14 +7588,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428806 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_Parental_RNAseq_24h_3\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428806\n SAMEA4608110\n \ E-MTAB-6681:G144_Parental_RNAseq_24h_3\n \n @@ -9047,57 +7603,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552512\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534091\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 1\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 1\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 20997810\n \n \n - \ ENA-BASE-COUNT\n 1585139118\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:29 GMT + - Wed, 17 May 2023 18:36:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9115,7 +7659,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -9175,7 +7719,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:29 GMT + - Wed, 17 May 2023 18:36:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9193,7 +7737,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -9229,7 +7773,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:31 GMT + - Wed, 17 May 2023 18:36:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9237,68 +7781,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534091&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrCMBAA0L1fEXDoVLlcc821u7NQnNyapKVKMCGNiHAfr298JzxDc5Qlv51c - 5hmpNzBqGYgGZmAWBM0d2A7Gm8YJzNQPd7HaUmAH3vWLCW4jo8nyitp4JEAQEQMg11f8qnavNR+t - yiXV5FNUj0MtMabPGtSWiqr7P5J7rr42PwAAAP//AwA2wGMdjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:31 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F25B101B32710000000000000001.m_1 - NCBI-SID: - - CC95F25B101B3271_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F25B101B3271_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:31 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -9309,29 +7791,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534091&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534091\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/001/ERR2534091/ERR2534091.fastq.gz\t989268235\t0802cce838493fc43f1633340c764b14\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534091/G144_Parental_RNAseq_24h_3.fastq.gz\t1030713695\tde063ac40bec17deefda44342353dd0d\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534091\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/001/ERR2534091/ERR2534091.fastq.gz\t989268235\t0802cce838493fc43f1633340c764b14\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534091/G144_Parental_RNAseq_24h_3.fastq.gz\t1030713695\tde063ac40bec17deefda44342353dd0d\tftp.sra.ebi.ac.uk/vol1/err/ERR253/001/ERR2534091\t655688068\tbde1222054dbb257c4c7c293285f348c\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '487' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:31 GMT + - Wed, 17 May 2023 18:36:26 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -9340,7 +7820,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -9351,7 +7831,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534092 response: @@ -9393,7 +7873,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:33 GMT + - Wed, 17 May 2023 18:36:26 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9411,7 +7891,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552513 response: @@ -9479,7 +7959,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:33 GMT + - Wed, 17 May 2023 18:36:27 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9497,14 +7977,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428807 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_Parental_RNAseq_4h_1\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428807\n SAMEA4608111\n \ E-MTAB-6681:G144_Parental_RNAseq_4h_1\n \n @@ -9512,57 +7992,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552513\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534092\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 1\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 1\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 21890892\n \n \n - \ ENA-BASE-COUNT\n 1652585468\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:34 GMT + - Wed, 17 May 2023 18:36:26 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9580,7 +8048,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -9640,7 +8108,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:35 GMT + - Wed, 17 May 2023 18:36:27 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9658,7 +8126,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -9694,7 +8162,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:35 GMT + - Wed, 17 May 2023 18:36:28 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9702,68 +8170,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534092&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIvQrCMBAA4L1PEXDoVLlL0jTX3VkoTm75K1WCKWlEhHt4/cbvJM/QHdXtb8+X - ZZGj0kCSjdWEdhqRJaAdYBqAbihnMDOoO6cUEnnlkUZwGA1RXFWyRkfydsXAzBqAr6/8Ff3W2n70 - Yq+llVCyeBzC5Vw+KYq1VNG2fxT/TKF1PwAAAP//AwDBPahJjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:36 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42164101B32C10000000000000001.m_1 - NCBI-SID: - - C7A42164101B32C1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42164101B32C1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:36 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -9774,29 +8180,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534092&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534092\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/002/ERR2534092/ERR2534092.fastq.gz\t1032472887\t653c55e458a2554089c39a4e18d70927\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534092/G144_Parental_RNAseq_4h_1.fastq.gz\t1075913209\t6d410b59ed993b1c515ec502cad05534\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534092\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/002/ERR2534092/ERR2534092.fastq.gz\t1032472887\t653c55e458a2554089c39a4e18d70927\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534092/G144_Parental_RNAseq_4h_1.fastq.gz\t1075913209\t6d410b59ed993b1c515ec502cad05534\tftp.sra.ebi.ac.uk/vol1/err/ERR253/002/ERR2534092\t684918731\t8180fa815cec978c15eaaefb49009f50\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '487' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:42 GMT + - Wed, 17 May 2023 18:36:28 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -9805,7 +8209,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -9816,7 +8220,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534093 response: @@ -9858,7 +8262,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:42 GMT + - Wed, 17 May 2023 18:36:29 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9876,7 +8280,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552514 response: @@ -9944,7 +8348,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:43 GMT + - Wed, 17 May 2023 18:36:29 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9962,14 +8366,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428808 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_Parental_RNAseq_4h_2\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428808\n SAMEA4608112\n \ E-MTAB-6681:G144_Parental_RNAseq_4h_2\n \n @@ -9977,57 +8381,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). - GFP-positive cells were single cell sorted 48h post-transfection, expanded - and screened by immunoblotting for CIC deletion. Inhibitor treatment with - 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures - for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552514\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534093\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + GFP-positive cells were single cell sorted 48h post-transfection, expanded + and screened by immunoblotting for CIC deletion. Inhibitor treatment with + 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures + for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 1\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 1\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 20416177\n \n \n - \ ENA-BASE-COUNT\n 1541046510\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:43 GMT + - Wed, 17 May 2023 18:36:30 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10045,7 +8437,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -10105,7 +8497,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:43 GMT + - Wed, 17 May 2023 18:36:30 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10123,7 +8515,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -10159,7 +8551,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:44 GMT + - Wed, 17 May 2023 18:36:30 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10167,68 +8559,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534093&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzLsQrDIBAA0D1fIXTIlHKep9HsnQuhUzdjlLRIDImlFO7jm/XBu+AVmmP322fi - 2ziiVgROsVE9KmOkZQRpO+g7cA+JA5gB6cnKJKKAErWO1iZno0s6kHFz8HRGZiYAvq/5J9ql1u1o - xbaXWkLJ4nUIn3P5xlmksou6nFCmdwy1+QMAAP//AwC4yyTmjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:45 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A421B2101B33510000000000000001.m_1 - NCBI-SID: - - C7A421B2101B3351_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A421B2101B3351_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:45 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -10239,29 +8569,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534093&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534093\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/003/ERR2534093/ERR2534093.fastq.gz\t961178965\t6e274ff76681397696f3da627dabb387\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534093/G144_Parental_RNAseq_4h_2.fastq.gz\t1001231193\t1d9adb545303b3a0d7e9f20ca98f45d7\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534093\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/003/ERR2534093/ERR2534093.fastq.gz\t961178965\t6e274ff76681397696f3da627dabb387\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534093/G144_Parental_RNAseq_4h_2.fastq.gz\t1001231193\t1d9adb545303b3a0d7e9f20ca98f45d7\tftp.sra.ebi.ac.uk/vol1/err/ERR253/003/ERR2534093\t637236598\t76040bde5e5f7e6ec50451975626a15a\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '486' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:46 GMT + - Wed, 17 May 2023 18:36:30 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -10270,7 +8598,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -10281,7 +8609,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534094 response: @@ -10323,7 +8651,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:46 GMT + - Wed, 17 May 2023 18:36:31 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10341,7 +8669,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552515 response: @@ -10409,7 +8737,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:47 GMT + - Wed, 17 May 2023 18:36:32 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10427,14 +8755,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428809 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_Parental_RNAseq_4h_3\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428809\n SAMEA4608113\n \ E-MTAB-6681:G144_Parental_RNAseq_4h_3\n \n @@ -10442,57 +8770,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552515\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534094\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 1\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 1\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 21534233\n \n \n - \ ENA-BASE-COUNT\n 1625567863\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:48 GMT + - Wed, 17 May 2023 18:36:32 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10510,7 +8826,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -10570,7 +8886,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:48 GMT + - Wed, 17 May 2023 18:36:32 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10588,7 +8904,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -10624,7 +8940,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:48 GMT + - Wed, 17 May 2023 18:36:33 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10632,68 +8948,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534094&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzLsQrDIBCA4T1PIXTIlHJnPDXZOxdCp24aLWmRGtRSCvfwzfbzwX+SZ+hqcfvH - 82VZJI0KJsXaoFUKYGIJaAcwA0w3lDPQTHjnMToywRFpb9FHixS1CTqEeBSCYeZj5us7/US/tbbX - Xuwlt7zmJJ5VuJTyNwbxyEW07YDsX3Ft3R8AAP//AwCBx/lKjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:49 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F2CE101B33910000000000000001.m_1 - NCBI-SID: - - CC95F2CE101B3391_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F2CE101B3391_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:49 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -10704,29 +8958,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534094&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534094\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/004/ERR2534094/ERR2534094.fastq.gz\t1013301291\ta4f593c11cf4db4f5a141897ad94274c\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534094/G144_Parental_RNAseq_4h_3.fastq.gz\t1055226153\t3dad11032609517b430aab1d1df2835c\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534094\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/004/ERR2534094/ERR2534094.fastq.gz\t1013301291\ta4f593c11cf4db4f5a141897ad94274c\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534094/G144_Parental_RNAseq_4h_3.fastq.gz\t1055226153\t3dad11032609517b430aab1d1df2835c\tftp.sra.ebi.ac.uk/vol1/err/ERR253/004/ERR2534094\t671843989\ta3fc50904843ae9b102922429126c0cf\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '487' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:50 GMT + - Wed, 17 May 2023 18:36:33 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -10735,7 +8987,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -10746,7 +8998,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534095 response: @@ -10788,7 +9040,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:51 GMT + - Wed, 17 May 2023 18:36:33 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10806,7 +9058,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552516 response: @@ -10874,7 +9126,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:52 GMT + - Wed, 17 May 2023 18:36:34 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10892,14 +9144,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428810 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_WTclone_RNAseq_24h_1\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428810\n SAMEA4608114\n \ E-MTAB-6681:G144_WTclone_RNAseq_24h_1\n \n @@ -10907,57 +9159,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552516\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534095\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 2\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 2\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 21431687\n \n \n - \ ENA-BASE-COUNT\n 1618365016\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:53 GMT + - Wed, 17 May 2023 18:36:34 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10975,7 +9215,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -11035,7 +9275,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:53 GMT + - Wed, 17 May 2023 18:36:34 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11053,7 +9293,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -11089,7 +9329,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:54 GMT + - Wed, 17 May 2023 18:36:35 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11097,68 +9337,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534095&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIuwrCMBQA0L1fEXDoVLmPJE26OwvFya1NE6oEU9qICPfj9YznRGdojn3a3rNc - xpEMa/BGetQ9WwYWAnQd9B34G9IAbjB8l5AcTt5iSsyMRGg5RgyowdLiyIiIBpDrK39Vu9a6Ha3a - 9lJLKFk9DjXlXD5xUansqq7/KPMzhtr8AAAA//8DAD7fHNyOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:54 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A421F0101B33E10000000000000001.m_1 - NCBI-SID: - - C7A421F0101B33E1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A421F0101B33E1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:54 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -11169,29 +9347,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534095&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534095\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/005/ERR2534095/ERR2534095.fastq.gz\t1071271905\tf7df87aa02b5689dae89556042df7223\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534095/G144_WTclone_RNAseq_24h_1.fastq.gz\t1116056605\tc410683ea6b481add8548e826b53091d\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534095\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/005/ERR2534095/ERR2534095.fastq.gz\t1071271905\tf7df87aa02b5689dae89556042df7223\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534095/G144_WTclone_RNAseq_24h_1.fastq.gz\t1116056605\tc410683ea6b481add8548e826b53091d\tftp.sra.ebi.ac.uk/vol1/err/ERR253/005/ERR2534095\t714736283\tbcef41770242d71d9ffaa3c0b9a9b50d\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '487' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:55 GMT + - Wed, 17 May 2023 18:36:35 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -11200,7 +9376,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -11211,7 +9387,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534096 response: @@ -11253,7 +9429,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:56 GMT + - Wed, 17 May 2023 18:36:35 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11271,7 +9447,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552517 response: @@ -11339,7 +9515,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:56 GMT + - Wed, 17 May 2023 18:36:36 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11357,14 +9533,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428811 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_WTclone_RNAseq_24h_2\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428811\n SAMEA4608115\n \ E-MTAB-6681:G144_WTclone_RNAseq_24h_2\n \n @@ -11372,57 +9548,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552517\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534096\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 2\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 2\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 20910521\n \n \n - \ ENA-BASE-COUNT\n 1578993339\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:57 GMT + - Wed, 17 May 2023 18:36:36 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11440,7 +9604,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -11500,7 +9664,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:58 GMT + - Wed, 17 May 2023 18:36:37 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11518,7 +9682,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -11554,7 +9718,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:42:58 GMT + - Wed, 17 May 2023 18:36:37 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11562,68 +9726,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534096&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzLvQrCMBAA4L1PEXDoVLk789N0dxaKk1uaplQJJiQREe7h7frBd6IzdLW4/Fn4 - Os+kLhKsZm0VISplmADHAcwA9o40gZ3APFihkx7ISjXSiEB61YCbcwGP55CYWQLw7R1/ot9by7UX - uaSWfIriWYWLMX3DKrZURNsPSMsr+Nb9AQAA//8DAJ9eOruOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:42:59 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42211101B34310000000000000001.m_1 - NCBI-SID: - - C7A42211101B3431_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42211101B3431_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:42:59 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -11634,29 +9736,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534096&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534096\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/006/ERR2534096/ERR2534096.fastq.gz\t1042880074\tec7e2a6c4f5a25107d99a50c115d4f7c\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534096/G144_WTclone_RNAseq_24h_2.fastq.gz\t1086208196\teb01cfdff7dfd39cfd30e5a6be2045ef\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534096\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/006/ERR2534096/ERR2534096.fastq.gz\t1042880074\tec7e2a6c4f5a25107d99a50c115d4f7c\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534096/G144_WTclone_RNAseq_24h_2.fastq.gz\t1086208196\teb01cfdff7dfd39cfd30e5a6be2045ef\tftp.sra.ebi.ac.uk/vol1/err/ERR253/006/ERR2534096\t695211537\t2d925f829c915f6865142e9fa914b7b7\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '487' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:42:59 GMT + - Wed, 17 May 2023 18:36:38 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -11665,7 +9765,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -11676,7 +9776,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534097 response: @@ -11718,7 +9818,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:00 GMT + - Wed, 17 May 2023 18:36:38 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11736,7 +9836,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552518 response: @@ -11804,7 +9904,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:01 GMT + - Wed, 17 May 2023 18:36:39 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11822,14 +9922,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428812 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_WTclone_RNAseq_4h_1\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428812\n SAMEA4608116\n \ E-MTAB-6681:G144_WTclone_RNAseq_4h_1\n \n @@ -11837,57 +9937,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552518\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534097\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 2\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 2\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 25292323\n \n \n - \ ENA-BASE-COUNT\n 1909946064\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:02 GMT + - Wed, 17 May 2023 18:36:38 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11905,7 +9993,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -11965,7 +10053,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:02 GMT + - Wed, 17 May 2023 18:36:39 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11983,7 +10071,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -12019,7 +10107,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:03 GMT + - Wed, 17 May 2023 18:36:39 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12027,68 +10115,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534097&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrDIBAA0D1fIXTIlHKeZ9TsnQuhUzc1SlqkhsRSCvfx7RvfCc/QHbvf3oEv - 84xaETjDlpAMKiBGkHYAM4C7SZwkTGTvTKPXUS9JG59jyjbIMVqk7DSkrFVmZgLg66t8Rb+2th29 - 2PbaaqxFPA7hS6mftIhcd9HWf9TwTLF1PwAAAP//AwCFY2v6jgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:43:04 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F426101B34810000000000000001.m_1 - NCBI-SID: - - CC95F426101B3481_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F426101B3481_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:04 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -12099,29 +10125,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534097&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534097\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/007/ERR2534097/ERR2534097.fastq.gz\t1263173701\t131910cd72175e2b1218785a96cf6eff\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534097/G144_WTclone_RNAseq_4h_1.fastq.gz\t1316839373\ta47d39765d40e29c4806c97d6c1b7e8a\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534097\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/007/ERR2534097/ERR2534097.fastq.gz\t1263173701\t131910cd72175e2b1218785a96cf6eff\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534097/G144_WTclone_RNAseq_4h_1.fastq.gz\t1316839373\ta47d39765d40e29c4806c97d6c1b7e8a\tftp.sra.ebi.ac.uk/vol1/err/ERR253/007/ERR2534097\t842472284\taa05d164f498e7b9a8bdacf95cf71358\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '486' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:04 GMT + - Wed, 17 May 2023 18:36:41 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -12130,7 +10154,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -12141,7 +10165,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERR2534098 response: @@ -12183,7 +10207,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:06 GMT + - Wed, 17 May 2023 18:36:41 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12201,7 +10225,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERX2552519 response: @@ -12269,7 +10293,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:06 GMT + - Wed, 17 May 2023 18:36:41 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12287,14 +10311,14 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERS2428813 response: body: string: "\n\n\n \n + alias=\"E-MTAB-6681:G144_WTclone_RNAseq_4h_2\" center_name=\"BRIC - Biotech + Research and Innovation Centre\" broker_name=\"ArrayExpress\">\n \n \ ERS2428813\n SAMEA4608117\n \ E-MTAB-6681:G144_WTclone_RNAseq_4h_2\n \n @@ -12302,57 +10326,45 @@ interactions: \ Homo sapiens\n human\n \ \n Protocols: CRISPR mediated KO of CIC: sgRNAs (Target sequence: mESCs: GCCTTCATGATCTTCAGCAAG; G144: GGGCGAGTGGTGGTATGCCC) - \ were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into - mESCs (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). + were cloned into pSpCas9(BB)-2A-GFP (Addgene 48138) and transfected into mESCs + (Lipofectamine 2000) or G144 cells (Amaxa Nucleofector II, Program A033). GFP-positive cells were single cell sorted 48h post-transfection, expanded and screened by immunoblotting for CIC deletion. Inhibitor treatment with 1 \u03BCM MEK inhibitor (PD0325901) was performed in 80% confluent cultures for 4 and 24 h respectively. DMSO was used as mock control. Cells were washed - once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted - using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library - preparation using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer - recommendations.\n \n \n - \ \n ENA-STUDY\n ERP108370\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ ERX2552519\n \n \n - \ \n \n ENA-RUN\n - \ ERR2534098\n \n \n - \ \n \n ENA-SUBMISSION\n - \ ERA1350207\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n isolate\n - \ not applicable\n \n - \ \n organism\n Homo - sapiens\n \n \n - \ organism part\n brain\n - \ \n \n cell_type\n - \ glial cell\n \n - \ \n cell_line\n G144\n + once with PBS, lysed directly in RLT buffer (Qiagen). Total RNA was extracted + using the RNAeasy kit (Qiagen). 500 ng of total RNA was used for library preparation + using TruSeq RNA Library Prep Kit v2 (Illumina) according to manufacturer + recommendations.\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n ENA-CHECKLIST\n ERC000011\n \ \n \n disease\n \ glioblastoma multiforme\n \n + \ \n individual\n 2\n + \ \n \n organism\n + \ Homo sapiens\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ cell type\n glial cell\n + \ \n \n common + name\n human\n \n + \ \n organism part\n brain\n + \ \n \n cell + line\n G144\n \n \ \n genotype\n wild type genotype\n \n \n - \ individual\n 2\n \n - \ \n ENA-CHECKLIST\n ERC000011\n - \ \n \n ENA-SPOT-COUNT\n - \ 22815094\n \n \n - \ ENA-BASE-COUNT\n 1722860231\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-05-30\n \n - \ \n ENA-LAST-UPDATE\n + \ ENA-FIRST-PUBLIC\n 2018-05-30\n + \ \n \n ENA-LAST-UPDATE\n \ 2018-04-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:07 GMT + - Wed, 17 May 2023 18:36:41 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12370,7 +10382,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP108370 response: @@ -12430,7 +10442,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:07 GMT + - Wed, 17 May 2023 18:36:42 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12448,7 +10460,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERA1350207 response: @@ -12484,7 +10496,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:08 GMT + - Wed, 17 May 2023 18:36:43 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12492,68 +10504,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=ERR2534098&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIvQrCMBAA4L1PEXDoVLlLLn/dnYXi5NYmKVWCCW1EhHt4/cbvJM/QHftc3wtf - pklqReAdW0PKGdDAEtANYAfwN5Qj4ij1nUkhrR6NRnIRg1ySS5ZWY9OclFKRmQmAr6/8Ff3WWj16 - UffSSihZPA4x51w+KYq17KJt/yjLM4XW/QAAAP//AwA+9dR2jgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:43:08 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F471101B34C10000000000000001.m_1 - NCBI-SID: - - CC95F471101B34C1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F471101B34C1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:08 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -12564,29 +10514,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERR2534098&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534098\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/008/ERR2534098/ERR2534098.fastq.gz\t1145401804\t911c351cdd7ab7c10a10d06f0bc3e3ea\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534098/G144_WTclone_RNAseq_4h_2.fastq.gz\t1192847608\t01566d01b53ff691ae40cb37416c8335\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nERR2534098\tftp.sra.ebi.ac.uk/vol1/fastq/ERR253/008/ERR2534098/ERR2534098.fastq.gz\t1145401804\t911c351cdd7ab7c10a10d06f0bc3e3ea\t\t\t\tftp.sra.ebi.ac.uk/vol1/run/ERR253/ERR2534098/G144_WTclone_RNAseq_4h_2.fastq.gz\t1192847608\t01566d01b53ff691ae40cb37416c8335\tftp.sra.ebi.ac.uk/vol1/err/ERR253/008/ERR2534098\t764386030\tbcd1929ccf8e70fdbf8ef73684f2bbb1\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '486' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:09 GMT + - Wed, 17 May 2023 18:36:43 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -12595,5 +10543,5 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' version: 1 diff --git a/test_volume/cassettes/surveyor.sra.metadata_is_gathered_correctly.yaml b/test_volume/cassettes/surveyor.sra.metadata_is_gathered_correctly.yaml index c8e2e86a0..2e31cff57 100644 --- a/test_volume/cassettes/surveyor.sra.metadata_is_gathered_correctly.yaml +++ b/test_volume/cassettes/surveyor.sra.metadata_is_gathered_correctly.yaml @@ -9,7 +9,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR002116 response: @@ -42,7 +42,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:10 GMT + - Wed, 17 May 2023 18:36:44 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -60,7 +60,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX001563 response: @@ -116,7 +116,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:10 GMT + - Wed, 17 May 2023 18:36:44 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -134,48 +134,42 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS001521 response: body: string: "\n\n\n \n - \ DRS001521\n SAMD00004104\n - \ \n Gg_HH16_1_embryo_mRNAseq\n \n - \ 9031\n Gallus gallus\n - \ \n \n \n \n - \ ENA-STUDY\n DRP000595\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX001563\n \n \n - \ \n \n ENA-RUN\n - \ DRR002116\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA000567\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n sample_name\n + alias=\"SAMD00004104\" center_name=\"Group for Morphological Evolution, Center + for Developmental Biology, Kobe Institute, RIKEN\" broker_name=\"DDBJ\">\n + \ \n DRS001521\n SAMD00004104\n \n + \ Gg_HH16_1_embryo_mRNAseq\n \n 9031\n + \ Gallus gallus\n chicken\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n \ DRS001521\n \n \n - \ sample comment\n mRNAseq of - chicken at stage HH16 (biological replicate 1)\n \n - \ \n ENA-SPOT-COUNT\n 32568360\n - \ \n \n ENA-BASE-COUNT\n - \ 3256836000\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2013-07-20\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-08-24\n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Gallus gallus\n \n + \ \n sample comment\n mRNAseq + of chicken at stage HH16 (biological replicate 1)\n \n + \ \n sample name\n DRS001521\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2013-02-27\n + \ \n \n ENA-LAST-UPDATE\n + \ 2014-11-12\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:10 GMT + - Wed, 17 May 2023 18:36:44 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -193,7 +187,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP000595 response: @@ -236,7 +230,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:11 GMT + - Wed, 17 May 2023 18:36:44 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -254,7 +248,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA000567 response: @@ -293,7 +287,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:13 GMT + - Wed, 17 May 2023 18:36:45 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -301,66 +295,4 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR002116&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQ6CMBQF0J2vaOLAhLkttFBmdxPi5PZ40KBpbAM1xuR9vJ7xnMwZ1bFTfs9y - mSbAaO3EtKbrvRswiIFuG/QN2hvsaPVo7V0svOs4sPPwmpc5eLOSHYgogFiziHSAXF/xq+qtlHzU - Ku+pJE5RPQ5FMabPuqiQdlW2f6T5uXKpfgAAAP//AwDGlZjGjgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:43:13 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A422B4101B35110000000000000001.m_1 - NCBI-SID: - - C7A422B4101B3511_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A422B4101B3511_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:13 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK version: 1 diff --git a/test_volume/cassettes/surveyor.sra.srp_survey.yaml b/test_volume/cassettes/surveyor.sra.srp_survey.yaml index 26bcf888d..18c5eb77a 100644 --- a/test_volume/cassettes/surveyor.sra.srp_survey.yaml +++ b/test_volume/cassettes/surveyor.sra.srp_survey.yaml @@ -9,7 +9,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP068364 response: @@ -57,7 +57,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:16 GMT + - Wed, 17 May 2023 18:36:48 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -75,7 +75,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR3098579 response: @@ -119,7 +119,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:17 GMT + - Wed, 17 May 2023 18:36:49 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -137,7 +137,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX1528546 response: @@ -185,7 +185,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:18 GMT + - Wed, 17 May 2023 18:36:49 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -203,52 +203,44 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS1246178 response: body: string: "\n\n\n \n SRS1246178\n + alias=\"GSM2037738\" center_name=\"Michael Pack, Medicine/DIgestive Diseases, + University of Pennsylvania\" broker_name=\"NCBI\">\n \n SRS1246178\n \ SAMN04397505\n - \ GSM2037738\n GSM2037738\n \n Control - 1\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP068364\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX1528546\n \n \n - \ \n \n ENA-RUN\n - \ SRR3098579\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA333195\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-76780\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ liver\n \n \n - \ tissue\n liver\n \n + \ GSM2037738\n \n + \ Control 1\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n \ \n treatment\n DMSO\n - \ \n \n ENA-SPOT-COUNT\n - \ 56576210\n \n \n - \ ENA-BASE-COUNT\n 11315242000\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2017-11-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2017-11-07\n \n + \ \n \n INSDC + secondary accession\n SRS1246178\n \n + \ \n NCBI submission package\n + \ Generic.1.0\n \n + \ \n organism\n Danio + rerio\n \n \n + \ tissue\n liver\n \n + \ \n source_name\n liver\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2017-08-24\n + \ \n \n ENA-LAST-UPDATE\n + \ 2017-08-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:18 GMT + - Wed, 17 May 2023 18:36:50 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -266,7 +258,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP068364 response: @@ -314,7 +306,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:19 GMT + - Wed, 17 May 2023 18:36:50 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -332,7 +324,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA333195 response: @@ -369,7 +361,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:19 GMT + - Wed, 17 May 2023 18:36:50 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -377,68 +369,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR3098579&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISwrCMBAA0H1PEXDRVWXyz/QSQnXlrk0iVUITkogIc3h9y3cSZxhaXct7o+uy - SECnLZJBYxUCgiMB3EzAJy5uHGeJs5Z3sk4ZAxY8Omm3oHkwEnX0MirhPAYiUgB0OdKXjXvvpY2s - 1Nyzz4k9G1tTyp8Y2CNX1vd/5O0VfR9+AAAA//8DANXTVuiPAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:43:21 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A422DB101B35910000000000000001.m_1 - NCBI-SID: - - C7A422DB101B3591_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A422DB101B3591_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:21 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -449,7 +379,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&id=27102575&retmode=json&tool=refinebio&email=hello@refine.bio response: @@ -459,21 +389,21 @@ interactions: e3rSkyYYCHuy0EzgT4agAXJhHNGeoIAfsk6yjrB8nMFvATaKyqfmIHsHzR7qRVXW88Ucvoq/+qpH 250UYMKhR58W1GX1JDZkIqR/dBW0qKooOA62S/iNDHpW/J3yYPAD27R3Ao1j0ncDstjetNsFy0tb ZJ0KzpO9RInx76Z3tqS9aDNtn1IdpXbieZVpXKPthvhIEi/rTOurVIqckZSfFqU7xDf2DzupR9Ti - I3PAhkXuoS2zaJdnAAAA///MVsty2yAU3fcrGK+SmdhGkm1ZziqvaZKpM5l02ekCIxTTIOEi5Kbp - 5N97APlVJzPxrisJuId7uI8Dh/qZy4pcHgi61wbT5Pbh0KgIpWry8PlA2Lli0pCbQ0ky/kQ+lnG0 - 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'*' @@ -488,18 +418,20 @@ interactions: Content-Type: - application/json; charset=UTF-8 Date: - - Wed, 28 Jul 2021 19:43:20 GMT + - Wed, 17 May 2023 18:36:51 GMT Keep-Alive: - timeout=4, max=40 NCBI-PHID: - - D0BD3D622C4BDF7500004EFC6D94BF07.1.1.m_1 + - 322C36E9C9D0D90500005409C5B33DFE.1.1.m_1 NCBI-SID: - - F6183D46F2126CAE_F0A8SID + - B9B997ADC5BB1F2E_0B63SID + Referrer-Policy: + - origin-when-cross-origin Server: - Finatra Set-Cookie: - - ncbi_sid=F6183D46F2126CAE_F0A8SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:21 GMT + - ncbi_sid=B9B997ADC5BB1F2E_0B63SID; domain=.nih.gov; path=/; expires=Fri, 17 + May 2024 18:36:51 GMT Strict-Transport-Security: - max-age=31536000; includeSubDomains; preload Transfer-Encoding: @@ -527,29 +459,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR3098579&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR3098579\tftp.sra.ebi.ac.uk/vol1/fastq/SRR309/009/SRR3098579/SRR3098579_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR309/009/SRR3098579/SRR3098579_2.fastq.gz\t3999026139;3956970849\t9dc519ddc6a0aed81998137fcefa16f2;e7c56cceed78f71188178b7a39204c87\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR3098579\tftp.sra.ebi.ac.uk/vol1/fastq/SRR309/009/SRR3098579/SRR3098579_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR309/009/SRR3098579/SRR3098579_2.fastq.gz\t3999026139;3956970849\t9dc519ddc6a0aed81998137fcefa16f2;e7c56cceed78f71188178b7a39204c87\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR309/009/SRR3098579\t6967490908\t78466070c9837bd51d6395ec3e428c9d\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '486' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:21 GMT + - Wed, 17 May 2023 18:36:51 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -558,7 +488,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -569,7 +499,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR3098580 response: @@ -613,7 +543,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:23 GMT + - Wed, 17 May 2023 18:36:51 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -631,7 +561,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX1528547 response: @@ -679,7 +609,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:23 GMT + - Wed, 17 May 2023 18:36:52 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -697,52 +627,44 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS1246177 response: body: string: "\n\n\n \n SRS1246177\n + alias=\"GSM2037739\" center_name=\"Michael Pack, Medicine/DIgestive Diseases, + University of Pennsylvania\" broker_name=\"NCBI\">\n \n SRS1246177\n \ SAMN04397506\n - \ GSM2037739\n GSM2037739\n \n Control - 2\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP068364\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX1528547\n \n \n - \ \n \n ENA-RUN\n - \ SRR3098580\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA333195\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-76780\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ liver\n \n \n - \ tissue\n liver\n \n + \ GSM2037739\n \n + \ Control 2\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n \ \n treatment\n DMSO\n - \ \n \n ENA-SPOT-COUNT\n - \ 55503963\n \n \n - \ ENA-BASE-COUNT\n 11100792600\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2017-11-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2017-11-07\n \n + \ \n \n INSDC + secondary accession\n SRS1246177\n \n + \ \n NCBI submission package\n + \ Generic.1.0\n \n + \ \n organism\n Danio + rerio\n \n \n + \ tissue\n liver\n \n + \ \n source_name\n liver\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2017-08-24\n + \ \n \n ENA-LAST-UPDATE\n + \ 2017-08-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:23 GMT + - Wed, 17 May 2023 18:36:52 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -760,7 +682,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP068364 response: @@ -808,7 +730,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:24 GMT + - Wed, 17 May 2023 18:36:53 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -826,7 +748,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA333195 response: @@ -863,7 +785,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:24 GMT + - Wed, 17 May 2023 18:36:54 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -871,68 +793,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR3098580&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQ7CIBAA0L1fQeLQqeYOKBz9CZPq5Aa0TTVESMEYk/t4feM7yTN09fDlHfg6 - zwocjQRsiKQhNDiyBDQD4IDyJmFCM2l7Z61Wr8PmXERNTlkvbTBBw0JkXViQmTUAX17pK/q9tVJ7 - UY7ccsxJPKrwKeXPuogtH6Lt/8jhucbW/QAAAP//AwBpZSYsjwAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:43:25 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F552101B35D10000000000000001.m_1 - NCBI-SID: - - CC95F552101B35D1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F552101B35D1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:25 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -943,29 +803,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR3098580&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR3098580\tftp.sra.ebi.ac.uk/vol1/fastq/SRR309/000/SRR3098580/SRR3098580_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR309/000/SRR3098580/SRR3098580_2.fastq.gz\t4061413779;4030359471\t6a7008ed2b0ed60d82d6a89e406b6c04;343eb987bbca5ec7001f2303c1570b2b\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR3098580\tftp.sra.ebi.ac.uk/vol1/fastq/SRR309/000/SRR3098580/SRR3098580_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR309/000/SRR3098580/SRR3098580_2.fastq.gz\t4061413779;4030359471\t6a7008ed2b0ed60d82d6a89e406b6c04;343eb987bbca5ec7001f2303c1570b2b\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR309/000/SRR3098580\t6882681615\t43ea4bf99c148937a27b6b40d8879bd1\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '486' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:25 GMT + - Wed, 17 May 2023 18:36:54 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -974,7 +832,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -985,7 +843,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR3098581 response: @@ -1029,7 +887,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:26 GMT + - Wed, 17 May 2023 18:36:54 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1047,7 +905,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX1528548 response: @@ -1095,7 +953,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:27 GMT + - Wed, 17 May 2023 18:36:55 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1113,52 +971,44 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS1246176 response: body: string: "\n\n\n \n SRS1246176\n + alias=\"GSM2037740\" center_name=\"Michael Pack, Medicine/DIgestive Diseases, + University of Pennsylvania\" broker_name=\"NCBI\">\n \n SRS1246176\n \ SAMN04397507\n - \ GSM2037740\n GSM2037740\n \n Biliatresone - 1\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP068364\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX1528548\n \n \n - \ \n \n ENA-RUN\n - \ SRR3098581\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA333195\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-76780\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ liver\n \n \n - \ tissue\n liver\n \n + \ GSM2037740\n \n + \ Biliatresone 1\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n \ \n treatment\n Biliatresone\n - \ \n \n ENA-SPOT-COUNT\n - \ 56284823\n \n \n - \ ENA-BASE-COUNT\n 11256964600\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2017-11-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2017-11-07\n \n + \ \n \n INSDC + secondary accession\n SRS1246176\n \n + \ \n NCBI submission package\n + \ Generic.1.0\n \n + \ \n organism\n Danio + rerio\n \n \n + \ tissue\n liver\n \n + \ \n source_name\n liver\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2017-08-24\n + \ \n \n ENA-LAST-UPDATE\n + \ 2017-08-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:27 GMT + - Wed, 17 May 2023 18:36:55 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1176,7 +1026,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP068364 response: @@ -1224,7 +1074,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:27 GMT + - Wed, 17 May 2023 18:36:55 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1242,7 +1092,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA333195 response: @@ -1279,7 +1129,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:28 GMT + - Wed, 17 May 2023 18:36:55 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1287,68 +1137,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR3098581&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzKMQrDIBQA0D2nEDpkSvn6rdFcopB26qZGSYtUUUsp/MM36+OdxBmGVm35OLqt - K4LRF81JGSVnNWtEEsDVBHzi4s7NInEB8SDEaDYPKBC1PZbRPqKzKENEO2tPRBKAru/0Y+Pee2kj - KzX37HNiz8ZsSvkbNhZzZX0/ILtX8H34AwAA//8DANkLet6PAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:43:29 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F57C101B36110000000000000001.m_1 - NCBI-SID: - - CC95F57C101B3611_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F57C101B3611_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:29 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -1359,29 +1147,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR3098581&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR3098581\tftp.sra.ebi.ac.uk/vol1/fastq/SRR309/001/SRR3098581/SRR3098581_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR309/001/SRR3098581/SRR3098581_2.fastq.gz\t4144635913;4104985160\t08aeda9f65037f4ad5f509b7292fac64;2a49ff5629ed4f535f53bbec56cacac1\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR3098581\tftp.sra.ebi.ac.uk/vol1/fastq/SRR309/001/SRR3098581/SRR3098581_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR309/001/SRR3098581/SRR3098581_2.fastq.gz\t4144635913;4104985160\t08aeda9f65037f4ad5f509b7292fac64;2a49ff5629ed4f535f53bbec56cacac1\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR309/001/SRR3098581\t6964767833\t33f9dc032338a83398cf3ba34ef3a78c\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '486' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:29 GMT + - Wed, 17 May 2023 18:36:56 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -1390,7 +1176,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -1401,7 +1187,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR3098582 response: @@ -1445,7 +1231,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:30 GMT + - Wed, 17 May 2023 18:36:56 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1463,7 +1249,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX1528549 response: @@ -1511,7 +1297,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:30 GMT + - Wed, 17 May 2023 18:36:57 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1529,52 +1315,44 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS1246175 response: body: string: "\n\n\n \n SRS1246175\n + alias=\"GSM2037741\" center_name=\"Michael Pack, Medicine/DIgestive Diseases, + University of Pennsylvania\" broker_name=\"NCBI\">\n \n SRS1246175\n \ SAMN04397508\n - \ GSM2037741\n GSM2037741\n \n Biliatresone - 2\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP068364\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX1528549\n \n \n - \ \n \n ENA-RUN\n - \ SRR3098582\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA333195\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-76780\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ liver\n \n \n - \ tissue\n liver\n \n + \ GSM2037741\n \n + \ Biliatresone 2\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n \ \n treatment\n Biliatresone\n - \ \n \n ENA-SPOT-COUNT\n - \ 59107344\n \n \n - \ ENA-BASE-COUNT\n 11821468800\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2017-11-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2017-11-07\n \n + \ \n \n INSDC + secondary accession\n SRS1246175\n \n + \ \n NCBI submission package\n + \ Generic.1.0\n \n + \ \n organism\n Danio + rerio\n \n \n + \ tissue\n liver\n \n + \ \n source_name\n liver\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2017-08-24\n + \ \n \n ENA-LAST-UPDATE\n + \ 2017-08-24\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:31 GMT + - Wed, 17 May 2023 18:36:57 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1592,7 +1370,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP068364 response: @@ -1640,7 +1418,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:31 GMT + - Wed, 17 May 2023 18:36:57 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1658,7 +1436,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA333195 response: @@ -1695,7 +1473,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:32 GMT + - Wed, 17 May 2023 18:36:58 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1703,68 +1481,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR3098582&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISwrCMBAA0H1PEXDRVWVmMk2aXkKortxl+qFKMKWNiDCH17d8JzpDdexxe4te - h8FC6NqO1FtCJm47qwToGsAG6YahZ9sD3nWxjp2bPEVxwi3K7CcMwhJ9YIxWVRlAL6/0NfVaynbU - ZttzyWNO5nGYmFL+zJNZ8m7K+o8sz3ks1Q8AAP//AwAvJGGEjwAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:43:33 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A4234C101B36510000000000000001.m_1 - NCBI-SID: - - C7A4234C101B3651_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A4234C101B3651_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:33 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -1775,29 +1491,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR3098582&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR3098582\tftp.sra.ebi.ac.uk/vol1/fastq/SRR309/002/SRR3098582/SRR3098582_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR309/002/SRR3098582/SRR3098582_2.fastq.gz\t4316824267;4279937658\tf6c81ae89ce3d6da48175926d4503547;6315f2371bc413220feb23c1ac030c46\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR3098582\tftp.sra.ebi.ac.uk/vol1/fastq/SRR309/002/SRR3098582/SRR3098582_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR309/002/SRR3098582/SRR3098582_2.fastq.gz\t4316824267;4279937658\tf6c81ae89ce3d6da48175926d4503547;6315f2371bc413220feb23c1ac030c46\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR309/002/SRR3098582\t7321424583\tf36466d72ab6b451be7d19b4ba7941a3\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '486' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:33 GMT + - Wed, 17 May 2023 18:36:58 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -1806,7 +1520,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -1817,7 +1531,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -1865,7 +1579,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:35 GMT + - Wed, 17 May 2023 18:36:59 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1883,7 +1597,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818004 response: @@ -1924,7 +1638,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:35 GMT + - Wed, 17 May 2023 18:36:59 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1942,7 +1656,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996258 response: @@ -1999,7 +1713,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:36 GMT + - Wed, 17 May 2023 18:37:00 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2017,54 +1731,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347490 response: body: string: "\n\n\n \n SRS2347490\n - \ SAMN07343034\n - \ GSM2700326\n GSM2700326\n \n wt1aGFP_1_neg\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996258\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818004-SRR5818005\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700326\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347490\n SAMN07343034\n GSM2700326\n \n + \ wt1aGFP_1_neg\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347490\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 1\n \n + \ \n cell type\n wt1a-\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a-\n - \ \n \n pool\n - \ 1\n \n \n - \ ENA-SPOT-COUNT\n 27406051\n - \ \n \n ENA-BASE-COUNT\n - \ 1671769111\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:36 GMT + - Wed, 17 May 2023 18:37:01 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2082,7 +1782,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -2130,7 +1830,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:37 GMT + - Wed, 17 May 2023 18:37:01 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2148,7 +1848,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -2185,7 +1885,7 @@ interactions: Content-Type: - 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uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818004&result=read_run - response: - body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818004\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/004/SRR5818004/SRR5818004.fastq.gz\t672369314\tff777268831b4ce053e1320f3bd0994a\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818004\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/004/SRR5818004/SRR5818004.fastq.gz\t672369314\tff777268831b4ce053e1320f3bd0994a\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/004/SRR5818004\t484368617\t3ea332e3590ef2fec94e783e6268cf81\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:38 GMT + - Wed, 17 May 2023 18:37:02 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -2372,7 +2010,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -2383,7 +2021,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818005 response: @@ -2424,7 +2062,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:40 GMT + - Wed, 17 May 2023 18:37:02 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2442,7 +2080,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996258 response: @@ -2499,7 +2137,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:40 GMT + - Wed, 17 May 2023 18:37:02 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2517,54 +2155,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347490 response: body: string: "\n\n\n \n SRS2347490\n - \ SAMN07343034\n - \ GSM2700326\n GSM2700326\n \n wt1aGFP_1_neg\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996258\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818004-SRR5818005\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700326\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347490\n SAMN07343034\n GSM2700326\n \n + \ wt1aGFP_1_neg\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347490\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 1\n \n + \ \n cell type\n wt1a-\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a-\n - \ \n \n pool\n - \ 1\n \n \n - \ ENA-SPOT-COUNT\n 27406051\n - \ \n \n ENA-BASE-COUNT\n - \ 1671769111\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:41 GMT + - Wed, 17 May 2023 18:37:02 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2582,7 +2206,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -2630,7 +2254,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:41 GMT + - Wed, 17 May 2023 18:37:04 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2648,7 +2272,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -2685,7 +2309,7 @@ interactions: Content-Type: - 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Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F5E4101B36E10000000000000001.m_1 - NCBI-SID: - - CC95F5E4101B36E1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F5E4101B36E1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:42 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -2765,29 +2327,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818005&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818005\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/005/SRR5818005/SRR5818005.fastq.gz\t686245154\t5ce51c7ad9cacdab4fb9f3961373eb99\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818005\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/005/SRR5818005/SRR5818005.fastq.gz\t686245154\t5ce51c7ad9cacdab4fb9f3961373eb99\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/005/SRR5818005\t494652534\ta0f3c6969d3416d4055e7196d6130d5d\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:43 GMT + - Wed, 17 May 2023 18:37:05 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -2796,7 +2356,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -2807,7 +2367,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818006 response: @@ -2848,7 +2408,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:44 GMT + - Wed, 17 May 2023 18:37:05 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2866,7 +2426,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996259 response: @@ -2923,7 +2483,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:44 GMT + - Wed, 17 May 2023 18:37:05 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2941,54 +2501,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347492 response: body: string: "\n\n\n \n SRS2347492\n - \ SAMN07343033\n - \ GSM2700327\n GSM2700327\n \n wt1aGFP_1_pos\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996259\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818006-SRR5818007\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700327\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347492\n SAMN07343033\n GSM2700327\n \n + \ wt1aGFP_1_pos\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347492\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 1\n \n + \ \n cell type\n wt1a+\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a+\n - \ \n \n pool\n - \ 1\n \n \n - \ ENA-SPOT-COUNT\n 24149372\n - \ \n \n ENA-BASE-COUNT\n - \ 1473111692\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:45 GMT + - Wed, 17 May 2023 18:37:05 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3006,7 +2552,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -3054,7 +2600,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:45 GMT + - Wed, 17 May 2023 18:37:06 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3072,7 +2618,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -3109,7 +2655,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:46 GMT + - Wed, 17 May 2023 18:37:06 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3117,68 +2663,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818006&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQ7CIBAA0L1fQeLQqeYOehT6EybVyQ0opBoiTcEYk/t4feM7yTN09XD72/N1 - WcigAdA8SrKWFBBLwGmAaUC8SZyVmknfWRuwoH1Y0aNOFElK5RQYh0kZmhwzjwB8eeWv6LfW9tqL - /SithJLFowqXc/nEVaRyiLb9o/hnDK37AQAA//8DAJv5LpqOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:43:47 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42429101B37310000000000000001.m_1 - NCBI-SID: - - C7A42429101B3731_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42429101B3731_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:47 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -3189,29 +2673,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818006&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818006\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/006/SRR5818006/SRR5818006.fastq.gz\t625431044\te487dc5e2399d2144ad35a0fa0b126bc\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818006\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/006/SRR5818006/SRR5818006.fastq.gz\t625431044\te487dc5e2399d2144ad35a0fa0b126bc\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/006/SRR5818006\t425995305\t680906bcd1b16f5e5223a308a1f3857a\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:47 GMT + - Wed, 17 May 2023 18:37:06 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -3220,7 +2702,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -3231,7 +2713,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818007 response: @@ -3272,7 +2754,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:48 GMT + - Wed, 17 May 2023 18:37:07 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3290,7 +2772,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996259 response: @@ -3347,7 +2829,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:49 GMT + - Wed, 17 May 2023 18:37:07 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3365,54 +2847,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347492 response: body: string: "\n\n\n \n SRS2347492\n - \ SAMN07343033\n - \ GSM2700327\n GSM2700327\n \n wt1aGFP_1_pos\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996259\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818006-SRR5818007\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700327\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347492\n SAMN07343033\n GSM2700327\n \n + \ wt1aGFP_1_pos\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347492\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 1\n \n + \ \n cell type\n wt1a+\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a+\n - \ \n \n pool\n - \ 1\n \n \n - \ ENA-SPOT-COUNT\n 24149372\n - \ \n \n ENA-BASE-COUNT\n - \ 1473111692\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:50 GMT + - Wed, 17 May 2023 18:37:07 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3430,7 +2898,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -3478,7 +2946,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:50 GMT + - Wed, 17 May 2023 18:37:09 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3496,7 +2964,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -3533,7 +3001,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:51 GMT + - Wed, 17 May 2023 18:37:08 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3541,68 +3009,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818007&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISwrDIBAA0H1OIXSRVcr4mai5RCHtqjs1SlokSmIphTl885bvIq7QHburH0/3 - eUbDDYAmJUd7EkACuB5AD5w/BJ8kn9A+yUtUaJLyUkShk0Qc0+K8dWiTDc4QkQKg25Z/rF9bq0fP - 6l5aCSWz18FczuUbF5bKztp6RvHvGFr3BwAA//8DAAfy9XmOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:43:51 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F61E101B37710000000000000001.m_1 - NCBI-SID: - - CC95F61E101B3771_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F61E101B3771_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:43:51 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -3613,29 +3019,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818007&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818007\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/007/SRR5818007/SRR5818007.fastq.gz\t640925564\tadff66451ac81a93ba08a067ea78e492\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818007\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/007/SRR5818007/SRR5818007.fastq.gz\t640925564\tadff66451ac81a93ba08a067ea78e492\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/007/SRR5818007\t436999920\tb35458f4b32e27f3556fdab9a59f9ca8\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:43:52 GMT + - Wed, 17 May 2023 18:37:08 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -3644,7 +3048,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -3655,7 +3059,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818008 response: @@ -3696,7 +3100,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:53 GMT + - Wed, 17 May 2023 18:37:10 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3714,7 +3118,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996260 response: @@ -3771,7 +3175,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:53 GMT + - Wed, 17 May 2023 18:37:10 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3789,54 +3193,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347491 response: body: string: "\n\n\n \n SRS2347491\n - \ SAMN07343032\n - \ GSM2700328\n GSM2700328\n \n wt1aGFP_2_neg\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996260\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818008-SRR5818009\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700328\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347491\n SAMN07343032\n GSM2700328\n \n + \ wt1aGFP_2_neg\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347491\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 2\n \n + \ \n cell type\n wt1a-\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a-\n - \ \n \n pool\n - \ 2\n \n \n - \ ENA-SPOT-COUNT\n 22896376\n - \ \n \n ENA-BASE-COUNT\n - \ 1396678936\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:54 GMT + - Wed, 17 May 2023 18:37:10 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3854,7 +3244,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -3902,7 +3292,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:54 GMT + - Wed, 17 May 2023 18:37:11 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3920,7 +3310,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -3957,7 +3347,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:55 GMT + - Wed, 17 May 2023 18:37:11 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3966,7 +3356,7 @@ interactions: code: 200 message: '' - request: - body: acc=SRR5818008&accept-proto=fasp&version=2.0 + body: null headers: Accept: - '*/*' @@ -3974,92 +3364,28 @@ interactions: - gzip, deflate Connection: - keep-alive - Content-Length: - - '44' User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi + - python-requests/2.30.0 + method: GET + uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818008&result=read_run response: body: - string: !!binary | - H4sIAAAAAAAAAwzMOwrCQBAA0D6nWLBIFZmZ7GfMJYRoZbeTD1EWNyQrIszhTfuKd6IzVPsW14/o - re8dIwOwWmg944WdEmBoIDSId8Kuxc76h0JsJYgQkhMmS+LGOXg7I4uLNnjVYwC9vtPP1Esp616b - dcslDzmZ525iSvk7jWbOmynLAVle01CqPwAAAP//AwBuHZzBjgAAAA== + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818008\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/008/SRR5818008/SRR5818008.fastq.gz\t560733863\t3d0e7b490a27a68b25c95af49f959a88\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/008/SRR5818008\t403681985\t0a3b7bb2125b8242b5df764f18b5a476\n" headers: Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests + - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:01 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A4248D101B38110000000000000001.m_1 - NCBI-SID: - - C7A4248D101B3811_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A4248D101B3811_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:01 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK -- request: - body: null - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - User-Agent: - - python-requests/2.25.1 - method: GET - uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818008&result=read_run - response: - body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818008\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/008/SRR5818008/SRR5818008.fastq.gz\t560733863\t3d0e7b490a27a68b25c95af49f959a88\t\t\t\t\t\t\n" - headers: - Cache-Control: - - no-cache, no-store, max-age=0, must-revalidate - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:01 GMT - Expires: - - '0' - Pragma: - - no-cache - Server: - - Apache-Coyote/1.1 + - Wed, 17 May 2023 18:37:12 GMT + Expires: + - '0' + Pragma: + - no-cache Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -4068,7 +3394,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -4079,7 +3405,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818009 response: @@ -4120,7 +3446,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:02 GMT + - Wed, 17 May 2023 18:37:12 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4138,7 +3464,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996260 response: @@ -4195,7 +3521,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:02 GMT + - Wed, 17 May 2023 18:37:12 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4213,54 +3539,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347491 response: body: string: "\n\n\n \n SRS2347491\n - \ SAMN07343032\n - \ GSM2700328\n GSM2700328\n \n wt1aGFP_2_neg\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996260\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818008-SRR5818009\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700328\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347491\n SAMN07343032\n GSM2700328\n \n + \ wt1aGFP_2_neg\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347491\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 2\n \n + \ \n cell type\n wt1a-\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a-\n - \ \n \n pool\n - \ 2\n \n \n - \ ENA-SPOT-COUNT\n 22896376\n - \ \n \n ENA-BASE-COUNT\n - \ 1396678936\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:03 GMT + - Wed, 17 May 2023 18:37:12 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4278,7 +3590,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -4326,7 +3638,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:09 GMT + - Wed, 17 May 2023 18:37:13 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4344,7 +3656,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -4381,7 +3693,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:09 GMT + - Wed, 17 May 2023 18:37:13 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4389,68 +3701,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818009&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIwQrCMAwA0LtfUfCw0yRJ26zbTwjTk7dmrkwpdmwVEfLx+o7vSCc47Ftc36KX - cfQBA0CvDi16YNcrAXYtdC3ilXCwdnB8U0YvKUZiGyUESj0TBSteeE5OgFXVAej5lb+mWWpd98as - W6llKtk8dhNzLp/5blLZTF3+UeQ5T/XwAwAA//8DAFjevJKOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:10 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42541101B38A10000000000000001.m_1 - NCBI-SID: - - C7A42541101B38A1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42541101B38A1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:10 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -4461,29 +3711,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818009&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818009\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/009/SRR5818009/SRR5818009.fastq.gz\t573562587\t8a669d361beb0a193e85aec0e2c63792\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818009\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/009/SRR5818009/SRR5818009.fastq.gz\t573562587\t8a669d361beb0a193e85aec0e2c63792\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/009/SRR5818009\t413150649\t615bfaa263ab882f962283b5b6ef4b06\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:11 GMT + - Wed, 17 May 2023 18:37:14 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -4492,7 +3740,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -4503,7 +3751,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818010 response: @@ -4544,7 +3792,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:11 GMT + - Wed, 17 May 2023 18:37:14 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4562,7 +3810,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996261 response: @@ -4619,7 +3867,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:11 GMT + - Wed, 17 May 2023 18:37:14 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4637,54 +3885,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347493 response: body: string: "\n\n\n \n SRS2347493\n - \ SAMN07343031\n - \ GSM2700329\n GSM2700329\n \n wt1aGFP_2_pos\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996261\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818010-SRR5818011\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700329\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347493\n SAMN07343031\n GSM2700329\n \n + \ wt1aGFP_2_pos\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347493\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 2\n \n + \ \n cell type\n wt1a+\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a+\n - \ \n \n pool\n - \ 2\n \n \n - \ ENA-SPOT-COUNT\n 24971267\n - \ \n \n ENA-BASE-COUNT\n - \ 1523247287\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:12 GMT + - Wed, 17 May 2023 18:37:14 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4702,7 +3936,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -4750,7 +3984,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:13 GMT + - Wed, 17 May 2023 18:37:15 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4768,7 +4002,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -4805,7 +4039,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:13 GMT + - Wed, 17 May 2023 18:37:15 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4813,68 +4047,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818010&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrCMBAA0L1fEXDoVLlLk/TanxCqk1ub5KgSTGgjItzH6xvfSZ+hOfalvFe5 - zrMlJEAQY9ASOWtEAw4dDB3iTePU6wnwLpqC7RlG0wf2wbILMTDxSI6tceRFxADI5ZW+qt1qLUer - yp5r9jmpx6GWlPInBsV5V3X7R16f0dfmBwAA//8DADOotuKOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:14 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F705101B38E10000000000000001.m_1 - NCBI-SID: - - CC95F705101B38E1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F705101B38E1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:14 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -4885,29 +4057,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818010&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818010\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/000/SRR5818010/SRR5818010.fastq.gz\t648541432\tfc630cc3bad9750ba9269c4c0301b770\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818010\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/000/SRR5818010/SRR5818010.fastq.gz\t648541432\tfc630cc3bad9750ba9269c4c0301b770\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/000/SRR5818010\t441588654\t28d53f0943dfcd5f6dedf8f986f5468c\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:14 GMT + - Wed, 17 May 2023 18:37:16 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -4916,7 +4086,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -4927,7 +4097,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818011 response: @@ -4968,7 +4138,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:14 GMT + - Wed, 17 May 2023 18:37:17 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4986,7 +4156,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996261 response: @@ -5043,7 +4213,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:15 GMT + - Wed, 17 May 2023 18:37:17 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5061,54 +4231,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347493 response: body: string: "\n\n\n \n SRS2347493\n - \ SAMN07343031\n - \ GSM2700329\n GSM2700329\n \n wt1aGFP_2_pos\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996261\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818010-SRR5818011\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700329\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347493\n SAMN07343031\n GSM2700329\n \n + \ wt1aGFP_2_pos\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347493\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 2\n \n + \ \n cell type\n wt1a+\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a+\n - \ \n \n pool\n - \ 2\n \n \n - \ ENA-SPOT-COUNT\n 24971267\n - \ \n \n ENA-BASE-COUNT\n - \ 1523247287\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:16 GMT + - Wed, 17 May 2023 18:37:17 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5126,7 +4282,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -5174,7 +4330,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:17 GMT + - Wed, 17 May 2023 18:37:18 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5192,7 +4348,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -5229,7 +4385,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:17 GMT + - Wed, 17 May 2023 18:37:18 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5237,68 +4393,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818011&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISwrCMBAA0H1PEXDRVWVmmm8vIVRX7pqaUiU0IYmIMIfXt3wnOkNXy5Lfnq/z - rCxaQGSpRhiVsY4J0AxgBsQb4TTSBPbOWjtPagGQLsgNViQ0TiESSL1ZL5lZAvDliF/R763l2otc - UktriuJZxRJj+oSH2FIRbf9H8q+wtu4HAAD//wMAPDVhvI4AAAA= - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:18 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F721101B39210000000000000001.m_1 - NCBI-SID: - - CC95F721101B3921_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F721101B3921_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:18 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -5309,29 +4403,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818011&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818011\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/001/SRR5818011/SRR5818011.fastq.gz\t664607333\ta10130c4bf552c08705fe4965c086543\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818011\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/001/SRR5818011/SRR5818011.fastq.gz\t664607333\ta10130c4bf552c08705fe4965c086543\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/001/SRR5818011\t453035789\t669b25a0049e4f0c121795112046f8b4\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:18 GMT + - Wed, 17 May 2023 18:37:19 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -5340,7 +4432,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -5351,7 +4443,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818012 response: @@ -5392,7 +4484,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:19 GMT + - Wed, 17 May 2023 18:37:19 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5410,7 +4502,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996262 response: @@ -5467,7 +4559,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:20 GMT + - Wed, 17 May 2023 18:37:19 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5485,54 +4577,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347494 response: body: string: "\n\n\n \n SRS2347494\n - \ SAMN07343030\n - \ GSM2700330\n GSM2700330\n \n wt1aGFP_3_neg\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996262\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818012-SRR5818013\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700330\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347494\n SAMN07343030\n GSM2700330\n \n + \ wt1aGFP_3_neg\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347494\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 3\n \n + \ \n cell type\n wt1a-\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a-\n - \ \n \n pool\n - \ 3\n \n \n - \ ENA-SPOT-COUNT\n 24020655\n - \ \n \n ENA-BASE-COUNT\n - \ 1465259955\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:20 GMT + - Wed, 17 May 2023 18:37:19 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5550,7 +4628,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -5598,7 +4676,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:21 GMT + - Wed, 17 May 2023 18:37:20 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5616,7 +4694,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -5653,7 +4731,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:21 GMT + - Wed, 17 May 2023 18:37:20 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5661,68 +4739,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818012&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISw7CIBAA0H1PQeKiq5oBhl8vYVJduSsUUg0RQjHGZA6vb/lO4gzD0db69nRd - FmW5BS4IBQI6tIYEcDOBmTi/CT5LMYO9UwQVg5MgN6O9ljpJFb1CF1JSTktLRAhAl1f+snHvvR4j - q630Ekpmj4OtOZdP3FgqjfX9H8U/Y+jDDwAA//8DABfQB9eOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:22 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A428B6101B39610000000000000001.m_1 - NCBI-SID: - - C7A428B6101B3961_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A428B6101B3961_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:22 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -5733,29 +4749,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818012&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818012\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/002/SRR5818012/SRR5818012.fastq.gz\t596873240\t34bb91a39c5c162acd96838f5271558a\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818012\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/002/SRR5818012/SRR5818012.fastq.gz\t596873240\t34bb91a39c5c162acd96838f5271558a\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/002/SRR5818012\t424049487\te05ec9303d76b636f35eb549cff59638\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:22 GMT + - Wed, 17 May 2023 18:37:20 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -5764,7 +4778,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -5775,7 +4789,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818013 response: @@ -5816,7 +4830,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:23 GMT + - Wed, 17 May 2023 18:37:21 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5834,7 +4848,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996262 response: @@ -5891,7 +4905,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:24 GMT + - Wed, 17 May 2023 18:37:21 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5909,54 +4923,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347494 response: body: string: "\n\n\n \n SRS2347494\n - \ SAMN07343030\n - \ GSM2700330\n GSM2700330\n \n wt1aGFP_3_neg\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996262\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818012-SRR5818013\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700330\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347494\n SAMN07343030\n GSM2700330\n \n + \ wt1aGFP_3_neg\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347494\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 3\n \n + \ \n cell type\n wt1a-\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a-\n - \ \n \n pool\n - \ 3\n \n \n - \ ENA-SPOT-COUNT\n 24020655\n - \ \n \n ENA-BASE-COUNT\n - \ 1465259955\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:24 GMT + - Wed, 17 May 2023 18:37:22 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5974,7 +4974,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -6022,7 +5022,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:25 GMT + - Wed, 17 May 2023 18:37:22 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6040,7 +5040,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -6077,7 +5077,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:25 GMT + - Wed, 17 May 2023 18:37:22 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6085,68 +5085,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818013&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISwrCMBAA0H1PEXDRVWVmMmnaXkKortw1P6oEU9qICHN4fct3ojM0x75sbyfX - eTYDDoBaWBvoexxZCNB2YDvEG+GkcTJ8FzbOYEiEo7eRyFmNPBJjBEMhxSAiDCCXV/6qdq11O1q1 - 7aUWX7J6HGrJuXxiUKnsqq7/KO4ZfW1+AAAA//8DAL7QTpeOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:26 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F75F101B39A10000000000000001.m_1 - NCBI-SID: - - CC95F75F101B39A1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F75F101B39A1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:26 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -6157,29 +5095,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818013&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818013\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/003/SRR5818013/SRR5818013.fastq.gz\t611906971\tce77ccfd2bc4c23e9ee5501ca3e9243e\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818013\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/003/SRR5818013/SRR5818013.fastq.gz\t611906971\tce77ccfd2bc4c23e9ee5501ca3e9243e\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/003/SRR5818013\t435066194\t45b51df219c7e22b73149241e052dfed\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:26 GMT + - Wed, 17 May 2023 18:37:24 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -6188,7 +5124,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -6199,7 +5135,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818014 response: @@ -6240,7 +5176,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:27 GMT + - Wed, 17 May 2023 18:37:24 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6258,7 +5194,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996263 response: @@ -6315,7 +5251,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:28 GMT + - Wed, 17 May 2023 18:37:24 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6333,54 +5269,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347495 response: body: string: "\n\n\n \n SRS2347495\n - \ SAMN07343029\n - \ GSM2700331\n GSM2700331\n \n wt1aGFP_3_pos\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996263\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818014-SRR5818015\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700331\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347495\n SAMN07343029\n GSM2700331\n \n + \ wt1aGFP_3_pos\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347495\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 3\n \n + \ \n cell type\n wt1a+\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a+\n - \ \n \n pool\n - \ 3\n \n \n - \ ENA-SPOT-COUNT\n 25570895\n - \ \n \n ENA-BASE-COUNT\n - \ 1559824595\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:29 GMT + - Wed, 17 May 2023 18:37:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6398,7 +5320,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -6446,7 +5368,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:29 GMT + - Wed, 17 May 2023 18:37:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6464,7 +5386,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -6501,7 +5423,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:30 GMT + - Wed, 17 May 2023 18:37:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6509,68 +5431,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818014&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrCMBAA0L1fEXDoVLm73JHYnxCqk1ubVKqEJrQREe7j7fKGd6IzNPs2ls+k - t2EQjx6QlYWtt+JBCdB14DrEO2FvqWd5aLSOHI2RA7IEwDh7LzGAvUxCBKCqfHhd08+0S61lb03Z - cs0hJ/PazZhS/s7RPPNm6nJEnt5zqM0fAAD//wMALJ4MxY4AAAA= - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:30 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42964101B39E10000000000000001.m_1 - NCBI-SID: - - C7A42964101B39E1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42964101B39E1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:30 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -6581,29 +5441,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818014&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818014\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/004/SRR5818014/SRR5818014.fastq.gz\t653073940\t8ce9ecf3ee5c145072e81fdf9f4410b3\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818014\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/004/SRR5818014/SRR5818014.fastq.gz\t653073940\t8ce9ecf3ee5c145072e81fdf9f4410b3\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/004/SRR5818014\t454383580\td37272ad4c145c01de885dc039b52200\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:31 GMT + - Wed, 17 May 2023 18:37:25 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -6612,7 +5470,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -6623,7 +5481,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818015 response: @@ -6664,7 +5522,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:32 GMT + - Wed, 17 May 2023 18:37:26 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6682,7 +5540,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996263 response: @@ -6739,7 +5597,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:32 GMT + - Wed, 17 May 2023 18:37:26 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6757,54 +5615,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347495 response: body: string: "\n\n\n \n SRS2347495\n - \ SAMN07343029\n - \ GSM2700331\n GSM2700331\n \n wt1aGFP_3_pos\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996263\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818014-SRR5818015\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700331\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347495\n SAMN07343029\n GSM2700331\n \n + \ wt1aGFP_3_pos\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347495\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 3\n \n + \ \n cell type\n wt1a+\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a+\n - \ \n \n pool\n - \ 3\n \n \n - \ ENA-SPOT-COUNT\n 25570895\n - \ \n \n ENA-BASE-COUNT\n - \ 1559824595\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:33 GMT + - Wed, 17 May 2023 18:37:27 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6822,7 +5666,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -6870,7 +5714,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:34 GMT + - Wed, 17 May 2023 18:37:27 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6888,7 +5732,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -6925,7 +5769,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:34 GMT + - Wed, 17 May 2023 18:37:27 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -6933,68 +5777,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818015&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISwrCMBAA0H1PEXDRVWUm//QSQnXlLl+qBFPaiAhzeH3Ld+JnGI7db+9A12VR - Fi2gIqklOqGcIw5oJjAT4o3jLPiM7k6pZBt9FDIIpSH5Igwa6bBoy8ErT0QSgC6v+mXj2vt2jGzb - W2+xVfY4mK+1fXJipe2sr/9o4ZljH34AAAD//wMAgDyOdI4AAAA= - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:35 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F7B9101B3A310000000000000001.m_1 - NCBI-SID: - - CC95F7B9101B3A31_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F7B9101B3A31_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:35 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -7005,29 +5787,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818015&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818015\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/005/SRR5818015/SRR5818015.fastq.gz\t666679070\tcc72fb436d98e3577b504400bcc72c86\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818015\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/005/SRR5818015/SRR5818015.fastq.gz\t666679070\tcc72fb436d98e3577b504400bcc72c86\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/005/SRR5818015\t464193599\tdfe8cac34b3560daf3717491f6820a5a\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:35 GMT + - Wed, 17 May 2023 18:37:28 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -7036,7 +5816,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -7047,7 +5827,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818016 response: @@ -7088,7 +5868,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:36 GMT + - Wed, 17 May 2023 18:37:28 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7106,7 +5886,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996264 response: @@ -7163,7 +5943,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:37 GMT + - Wed, 17 May 2023 18:37:29 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7181,54 +5961,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347496 response: body: string: "\n\n\n \n SRS2347496\n - \ SAMN07343037\n - \ GSM2700332\n GSM2700332\n \n wt1aGFP_4_neg\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996264\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818016-SRR5818017\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700332\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347496\n SAMN07343037\n GSM2700332\n \n + \ wt1aGFP_4_neg\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347496\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 4\n \n + \ \n cell type\n wt1a-\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a-\n - \ \n \n pool\n - \ 4\n \n \n - \ ENA-SPOT-COUNT\n 28010081\n - \ \n \n ENA-BASE-COUNT\n - \ 1708614941\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:37 GMT + - Wed, 17 May 2023 18:37:29 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7246,7 +6012,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -7294,7 +6060,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:38 GMT + - Wed, 17 May 2023 18:37:29 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7312,7 +6078,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -7349,7 +6115,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:38 GMT + - Wed, 17 May 2023 18:37:30 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7357,68 +6123,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818016&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISwrCMBAA0H1PEXDRVSUzmebTSwjVlbskbakSTEgjIszh9S3fCc+yO6ov78DX - eR4tWAmayREp1NoySjCDNAPADWFSNI3uzls0ALSOZGFzxpGNAZWOC5oQVfCemUlKvrzSV/R7a+Xo - Ram55ZiTeBzCp5Q/6yK2XEXb/5HDc42t+wEAAP//AwCQxTb0jgAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:39 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A429C2101B3A710000000000000001.m_1 - NCBI-SID: - - C7A429C2101B3A71_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A429C2101B3A71_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:39 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -7429,29 +6133,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818016&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818016\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/006/SRR5818016/SRR5818016.fastq.gz\t692367575\ta4efd28f613dbea313df0ca2f608dd1c\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818016\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/006/SRR5818016/SRR5818016.fastq.gz\t692367575\ta4efd28f613dbea313df0ca2f608dd1c\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/006/SRR5818016\t494432668\tfc7114e5481f97948cb236cd27bc3baa\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:39 GMT + - Wed, 17 May 2023 18:37:31 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -7460,7 +6162,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -7471,7 +6173,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818017 response: @@ -7512,7 +6214,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:40 GMT + - Wed, 17 May 2023 18:37:30 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7530,7 +6232,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996264 response: @@ -7587,7 +6289,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:41 GMT + - Wed, 17 May 2023 18:37:31 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7605,54 +6307,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347496 response: body: string: "\n\n\n \n SRS2347496\n - \ SAMN07343037\n - \ GSM2700332\n GSM2700332\n \n wt1aGFP_4_neg\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996264\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818016-SRR5818017\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700332\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347496\n SAMN07343037\n GSM2700332\n \n + \ wt1aGFP_4_neg\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347496\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 4\n \n + \ \n cell type\n wt1a-\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a-\n - \ \n \n pool\n - \ 4\n \n \n - \ ENA-SPOT-COUNT\n 28010081\n - \ \n \n ENA-BASE-COUNT\n - \ 1708614941\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:41 GMT + - Wed, 17 May 2023 18:37:32 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7670,7 +6358,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -7718,7 +6406,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:43 GMT + - Wed, 17 May 2023 18:37:32 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7736,7 +6424,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -7773,7 +6461,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:43 GMT + - Wed, 17 May 2023 18:37:32 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7782,7 +6470,7 @@ interactions: code: 200 message: '' - request: - body: acc=SRR5818017&accept-proto=fasp&version=2.0 + body: null headers: Accept: - '*/*' @@ -7790,92 +6478,28 @@ interactions: - gzip, deflate Connection: - keep-alive - Content-Length: - - '44' User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi + - python-requests/2.30.0 + method: GET + uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818017&result=read_run response: body: - string: !!binary | - H4sIAAAAAAAAAwzIsQ7CIBAA0L1fQeLQqeYOOCj9CZPq5EYLphoihGKMyX28Hd87yTN0e/Xls/B1 - nmnEEdAygbUwKlQsDw5gB8SbxEnRpOWdUbvgSEUNRi5Bq0AUDKE3zhoZV8XMGoAv7/QT/dZa2XtR - am55zUk8d+FTyt8YxCNX0bYj8vKKa+v+AAAA//8DAOFojYaOAAAA + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818017\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/007/SRR5818017/SRR5818017.fastq.gz\t710518408\te4f4e0e59f8d282d8b307e33e2cef0e1\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/007/SRR5818017\t507708313\t149d953e4062bd43d55d651a69762ec3\n" headers: Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests + - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:44 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F819101B3AC10000000000000001.m_1 - NCBI-SID: - - CC95F819101B3AC1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F819101B3AC1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:44 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK -- request: - body: null - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - User-Agent: - - python-requests/2.25.1 - method: GET - uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818017&result=read_run - response: - body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818017\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/007/SRR5818017/SRR5818017.fastq.gz\t710518408\te4f4e0e59f8d282d8b307e33e2cef0e1\t\t\t\t\t\t\n" - headers: - Cache-Control: - - no-cache, no-store, max-age=0, must-revalidate - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:45 GMT - Expires: - - '0' - Pragma: - - no-cache - Server: - - Apache-Coyote/1.1 + - Wed, 17 May 2023 18:37:32 GMT + Expires: + - '0' + Pragma: + - no-cache Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -7884,7 +6508,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -7895,7 +6519,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818018 response: @@ -7936,7 +6560,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:45 GMT + - Wed, 17 May 2023 18:37:33 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -7954,7 +6578,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996265 response: @@ -8011,7 +6635,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:45 GMT + - Wed, 17 May 2023 18:37:34 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8029,54 +6653,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347497 response: body: string: "\n\n\n \n SRS2347497\n - \ SAMN07343036\n - \ GSM2700333\n GSM2700333\n \n wt1aGFP_4_pos\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996265\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818018-SRR5818019\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700333\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347497\n SAMN07343036\n GSM2700333\n \n + \ wt1aGFP_4_pos\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347497\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 4\n \n + \ \n cell type\n wt1a+\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a+\n - \ \n \n pool\n - \ 4\n \n \n - \ ENA-SPOT-COUNT\n 23930206\n - \ \n \n ENA-BASE-COUNT\n - \ 1459742566\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:47 GMT + - Wed, 17 May 2023 18:37:33 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8094,7 +6704,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -8142,7 +6752,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:47 GMT + - Wed, 17 May 2023 18:37:34 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8160,7 +6770,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -8197,7 +6807,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:47 GMT + - Wed, 17 May 2023 18:37:35 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8205,68 +6815,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818018&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISw6CMBAA0D2naOKCFWY+lI5cwgRduaMtBE1DCdQYkzm8vuU70RmqYx+3t9fb - MFhBARRtiTsRx6IE6BpwDeKdsGfq2T6UHaK4GVuEYIm9D/7SWSaJPMXYiaq2AHpd09fUSynbUZtt - zyWHnMzzMGNK+TNFM+fdlOUf2b+mUKofAAAA//8DAOFuAHyOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:48 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42A14101B3B010000000000000001.m_1 - NCBI-SID: - - C7A42A14101B3B01_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42A14101B3B01_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:48 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -8277,29 +6825,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818018&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818018\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/008/SRR5818018/SRR5818018.fastq.gz\t616926968\tad76198d7a162b845f421ab477807d57\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818018\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/008/SRR5818018/SRR5818018.fastq.gz\t616926968\tad76198d7a162b845f421ab477807d57\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/008/SRR5818018\t423688738\t371187f1410c523bbcb965328d3edd68\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:49 GMT + - Wed, 17 May 2023 18:37:35 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -8308,7 +6854,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -8319,7 +6865,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR5818019 response: @@ -8360,7 +6906,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:49 GMT + - Wed, 17 May 2023 18:37:36 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8378,7 +6924,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX2996265 response: @@ -8435,7 +6981,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:49 GMT + - Wed, 17 May 2023 18:37:35 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8453,54 +6999,40 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS2347497 response: body: string: "\n\n\n \n SRS2347497\n - \ SAMN07343036\n - \ GSM2700333\n GSM2700333\n \n wt1aGFP_4_pos\n - \ \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n SRP111553\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX2996265\n \n \n - \ \n \n ENA-RUN\n - \ SRR5818018-SRR5818019\n \n - \ \n \n \n - \ ENA-SUBMISSION\n SRA585736\n - \ \n \n \n - \ \n ARRAYEXPRESS\n E-GEOD-101204\n - \ \n \n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n + alias=\"GSM2700333\" center_name=\"Bioinformatics Unit, CNIC\" broker_name=\"NCBI\">\n + \ \n SRS2347497\n SAMN07343036\n GSM2700333\n \n + \ wt1aGFP_4_pos\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS2347497\n \n + \ \n organism\n Danio + rerio\n \n \n + \ pool\n 4\n \n + \ \n cell type\n wt1a+\n + \ \n \n source_name\n \ Heart\n \n \n - \ cell type\n wt1a+\n - \ \n \n pool\n - \ 4\n \n \n - \ ENA-SPOT-COUNT\n 23930206\n - \ \n \n ENA-BASE-COUNT\n - \ 1459742566\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-04-04\n \n + \ BioSampleModel\n Generic\n + \ \n \n ENA-FIRST-PUBLIC\n + \ 2018-03-22\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:51 GMT + - Wed, 17 May 2023 18:37:36 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8518,7 +7050,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP111553 response: @@ -8566,7 +7098,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:51 GMT + - Wed, 17 May 2023 18:37:37 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8584,7 +7116,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA585736 response: @@ -8621,7 +7153,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:52 GMT + - Wed, 17 May 2023 18:37:37 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8629,68 +7161,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR5818019&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzISwrCMBAA0H1PEXDRVWVmkpimlxCqK3f5lSqhCW1EhDm8vuU70Rm6Y3f17fk2 - z3rEEdCykoq0NUYzAZoBzIB4J5wkTTA+2Cm/BLxE7zxGtRiyQWlNSQar0UZkZgXA1y1/Rb+2Vo9e - 1L20EkoWz0O4nMsnRbGUXbT1H8W/UmjdDwAA//8DALIzE9+OAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:52 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42A3F101B3B410000000000000001.m_1 - NCBI-SID: - - C7A42A3F101B3B41_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42A3F101B3B41_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:52 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -8701,29 +7171,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR5818019&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818019\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/009/SRR5818019/SRR5818019.fastq.gz\t631703792\t4b04c53f6f028dc9fad6338662a5f1bc\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR5818019\tftp.sra.ebi.ac.uk/vol1/fastq/SRR581/009/SRR5818019/SRR5818019.fastq.gz\t631703792\t4b04c53f6f028dc9fad6338662a5f1bc\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR581/009/SRR5818019\t434259775\ta4bfc16dbab1d4f729c4552e3c9519d1\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '365' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:52 GMT + - Wed, 17 May 2023 18:37:38 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -8732,7 +7200,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -8743,7 +7211,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -8751,14 +7219,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -8767,7 +7237,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -8779,13 +7249,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:53 GMT + - Wed, 17 May 2023 18:37:37 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8803,7 +7273,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR051059 response: @@ -8837,7 +7307,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:54 GMT + - Wed, 17 May 2023 18:37:38 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8855,7 +7325,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX045951 response: @@ -8911,7 +7381,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:54 GMT + - Wed, 17 May 2023 18:37:39 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8929,55 +7399,43 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS057270 response: body: string: "\n\n\n \n - \ DRS057270\n SAMD00044993\n DRS057270\n \n Danio - rerio\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n DRP003977\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX045951\n \n \n - \ \n \n ENA-RUN\n - \ DRR051059\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA004269\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n description\n - \ Whole brain of adult zebrafish\n \n - \ \n bioproject_id\n PRJDB4470\n - \ \n \n sample_name\n - \ WB\n \n \n - \ sample_title\n zebrafish whole - brain\n \n \n - \ genotype\n WT\n \n - \ \n tissue_type\n brain\n - \ \n \n ENA-SPOT-COUNT\n - \ 125165088\n \n \n - \ ENA-BASE-COUNT\n 25283347776\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-01-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-01-07\n \n + alias=\"SAMD00044993\" center_name=\"National Institute of Genetics (Japan)\" + broker_name=\"DDBJ\">\n \n DRS057270\n + \ SAMD00044993\n + \ \n zebrafish whole brain\n \n + \ 7955\n Danio rerio\n + \ zebrafish\n \n Whole + brain of adult zebrafish\n \n \n + \ \n ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ DRS057270\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Danio rerio\n \n + \ \n tissue type\n brain\n + \ \n \n sample + name\n WB\n \n + \ \n genotype\n WT\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2018-01-06\n + \ \n \n ENA-LAST-UPDATE\n + \ 2018-01-06\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:55 GMT + - Wed, 17 May 2023 18:37:39 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -8995,7 +7453,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -9003,14 +7461,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -9019,7 +7479,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -9031,13 +7491,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:55 GMT + - Wed, 17 May 2023 18:37:40 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9055,7 +7515,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA004269 response: @@ -9089,7 +7549,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:56 GMT + - Wed, 17 May 2023 18:37:40 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9097,68 +7557,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR051059&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzJuwrCMBQA0L1fEXDoVLk3ryad3YXi5JbElCrBhCQiwv14u55z4mcYWnXl4+my - rqAQlCWcZ2ulAK6ROKCZACfQNzSLFIvid9JmlsGI44OOUYCVm3DaK8M5BiENEUkAur7Tj41776WN - rNTcc8iJPRtzKeVvfLAtV9b3A7J/xdCHPwAAAP//AwD5xduojwAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:44:57 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F88E101B3B910000000000000001.m_1 - NCBI-SID: - - CC95F88E101B3B91_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F88E101B3B91_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:44:57 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -9169,29 +7567,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=DRR051059&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051059\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051059/DRR051059_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051059/DRR051059_2.fastq.gz\t11191882751;11504627040\t5437a4dd2fe03230513793d9a5e1c999;5bc99fce97935ae4b0950745beeed48c\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051059\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051059/DRR051059_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051059/DRR051059_2.fastq.gz\t11191882751;11504627040\t5437a4dd2fe03230513793d9a5e1c999;5bc99fce97935ae4b0950745beeed48c\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/drr/DRR051/DRR051059\t17799430261\t6874c83261c6ee3094f3a6b58221c348\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '471' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:44:58 GMT + - Wed, 17 May 2023 18:37:41 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -9200,7 +7596,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -9211,7 +7607,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR051060 response: @@ -9245,7 +7641,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:59 GMT + - Wed, 17 May 2023 18:37:41 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9263,7 +7659,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX045952 response: @@ -9319,7 +7715,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:59 GMT + - Wed, 17 May 2023 18:37:41 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9337,55 +7733,43 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS057269 response: body: string: "\n\n\n \n - \ DRS057269\n SAMD00044992\n DRS057269\n \n Danio - rerio\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n DRP003977\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX045952\n \n \n - \ \n \n ENA-RUN\n - \ DRR051060\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA004270\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n description\n - \ Telencephalon of adult zebrafrish barin\n \n - \ \n bioproject_id\n PRJDB4470\n - \ \n \n sample_name\n - \ Tel\n \n \n - \ sample_title\n zebrafihsh telencephalon\n - \ \n \n genotype\n - \ WT\n \n \n - \ tissue_type\n brain\n - \ \n \n ENA-SPOT-COUNT\n - \ 99040238\n \n \n - \ ENA-BASE-COUNT\n 20006128076\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-01-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-01-07\n \n + alias=\"SAMD00044992\" center_name=\"National Institute of Genetics (Japan)\" + broker_name=\"DDBJ\">\n \n DRS057269\n + \ SAMD00044992\n + \ \n zebrafihsh telencephalon\n \n + \ 7955\n Danio rerio\n + \ zebrafish\n \n Telencephalon + of adult zebrafrish barin\n \n \n + \ \n ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ DRS057269\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Danio rerio\n \n + \ \n tissue type\n brain\n + \ \n \n sample + name\n Tel\n \n + \ \n genotype\n WT\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2018-01-06\n + \ \n \n ENA-LAST-UPDATE\n + \ 2018-01-06\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:44:59 GMT + - Wed, 17 May 2023 18:37:41 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9403,7 +7787,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -9411,14 +7795,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -9427,7 +7813,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -9439,13 +7825,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:00 GMT + - Wed, 17 May 2023 18:37:42 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9463,7 +7849,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA004270 response: @@ -9497,7 +7883,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:01 GMT + - Wed, 17 May 2023 18:37:43 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9505,68 +7891,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR051060&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrDIBAA0D1fIXTIlHKn0Wrm7oXQqZsaJS1SRS2lcB/fjO+d+BmGVm35OLqu - K0gEBYQzaKHxACcOqCfACdQd9SLUgvJBXqsguMQQLXijHDfiYiK33s6blaiIaAag2zv92Lj3XtrI - Ss09+5zYszGbUv6GjcVcWd+PyO4VfB/+AAAA//8DAGeGt82PAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:01 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F912101B3BD10000000000000001.m_1 - NCBI-SID: - - CC95F912101B3BD1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F912101B3BD1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:01 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -9577,29 +7901,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=DRR051060&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051060\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051060/DRR051060_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051060/DRR051060_2.fastq.gz\t8860709458;9122863611\tb02f28ce177a28116323c9d9b85086f3;41a443250ebd3dcf3d9c19e3a6bf4ea9\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051060\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051060/DRR051060_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051060/DRR051060_2.fastq.gz\t8860709458;9122863611\tb02f28ce177a28116323c9d9b85086f3;41a443250ebd3dcf3d9c19e3a6bf4ea9\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/drr/DRR051/DRR051060\t14083815102\tc86e3251efa0c96b29379f2aca4da516\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '469' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:02 GMT + - Wed, 17 May 2023 18:37:43 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -9608,7 +7930,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -9619,7 +7941,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR051061 response: @@ -9653,7 +7975,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:02 GMT + - Wed, 17 May 2023 18:37:44 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9671,7 +7993,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX045953 response: @@ -9728,7 +8050,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:03 GMT + - Wed, 17 May 2023 18:37:44 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9746,57 +8068,44 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS057274 response: body: string: "\n\n\n \n - \ DRS057274\n SAMD00044989\n DRS057274\n \n Danio - rerio\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n DRP003977\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX045953\n \n \n - \ \n \n ENA-RUN\n - \ DRR051061\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA004271\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n description\n - \ Collected from 10 adult fish by using FACS\n - \ \n \n bioproject_id\n - \ PRJDB4470\n \n \n - \ sample_name\n 231A_GFP_plus_WB\n - \ \n \n sample_title\n - \ GFP+ cells from whole brain of SAGFF(LF)231A;UAS:GFP - transgenic zebrafish\n \n \n - \ genotype\n SAGFF(LF)231A;UAS:GFP\n - \ \n \n tissue_type\n - \ brain\n \n \n - \ ENA-SPOT-COUNT\n 136153270\n - \ \n \n ENA-BASE-COUNT\n - \ 27230654000\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-01-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-01-07\n \n + alias=\"SAMD00044989\" center_name=\"National Institute of Genetics (Japan)\" + broker_name=\"DDBJ\">\n \n DRS057274\n + \ SAMD00044989\n + \ \n GFP+ cells from whole brain of SAGFF(LF)231A;UAS:GFP + transgenic zebrafish\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n Collected from 10 adult fish by using + FACS\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ DRS057274\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Danio rerio\n \n + \ \n tissue type\n brain\n + \ \n \n sample + name\n 231A_GFP_plus_WB\n \n + \ \n genotype\n SAGFF(LF)231A;UAS:GFP\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2018-01-06\n + \ \n \n ENA-LAST-UPDATE\n + \ 2018-01-06\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:04 GMT + - Wed, 17 May 2023 18:37:44 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9814,7 +8123,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -9822,14 +8131,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -9838,7 +8149,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -9850,13 +8161,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:04 GMT + - Wed, 17 May 2023 18:37:45 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9874,7 +8185,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA004271 response: @@ -9908,7 +8219,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:05 GMT + - Wed, 17 May 2023 18:37:46 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -9916,68 +8227,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR051061&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzJvQrCMBAA4L1PEXDoVLm7/Lazu1Cc3NIkUiWYkkZEuIe36/ed6AzdXv32Wfgy - z6ARDDJaqcGZUTpgAnQD4ADmhm5SeiJ5ZyJvZPIKQMdRaTIxJQwq2mBRRtLMfBxf3/kn+rW1be/F - VksroWTx3IXPuXxTFI9SRVsPKMsrhdb9AQAA//8DAB+PMvSPAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:05 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42B00101B3C110000000000000001.m_1 - NCBI-SID: - - C7A42B00101B3C11_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42B00101B3C11_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:05 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -9988,29 +8237,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=DRR051061&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051061\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051061/DRR051061_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051061/DRR051061_2.fastq.gz\t10995619492;10855326022\t4f996689219b960f252308db99f7be3b;dffc1260802754bb0bb1102f3351bec8\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051061\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051061/DRR051061_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051061/DRR051061_2.fastq.gz\t10995619492;10855326022\t4f996689219b960f252308db99f7be3b;dffc1260802754bb0bb1102f3351bec8\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/drr/DRR051/DRR051061\t17350869380\t22a63ea4005d94526dee1c4d7c713d25\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '471' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:05 GMT + - Wed, 17 May 2023 18:37:45 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -10019,7 +8266,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -10030,7 +8277,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR051062 response: @@ -10064,7 +8311,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:06 GMT + - Wed, 17 May 2023 18:37:46 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10082,7 +8329,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX045954 response: @@ -10139,7 +8386,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:06 GMT + - Wed, 17 May 2023 18:37:47 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10157,57 +8404,44 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS057271 response: body: string: "\n\n\n \n - \ DRS057271\n SAMD00044988\n DRS057271\n \n Danio - rerio\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n DRP003977\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX045954\n \n \n - \ \n \n ENA-RUN\n - \ DRR051062\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA004272\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n description\n - \ Collected from 40 adult fish by using FACS\n - \ \n \n bioproject_id\n - \ PRJDB4470\n \n \n - \ sample_name\n 120A_GFP_plus_Tel\n - \ \n \n sample_title\n - \ GFP+ cells from telencephalon of SAGFF(LF)120A;UAS:GFP - transgenic zebrafish\n \n \n - \ genotype\n SAGFF(LF)120A;UAS:GFP\n - \ \n \n tissue_type\n - \ brain\n \n \n - \ ENA-SPOT-COUNT\n 49090307\n - \ \n \n ENA-BASE-COUNT\n - \ 9916242014\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-01-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-01-07\n \n + alias=\"SAMD00044988\" center_name=\"National Institute of Genetics (Japan)\" + broker_name=\"DDBJ\">\n \n DRS057271\n + \ SAMD00044988\n + \ \n GFP+ cells from telencephalon of SAGFF(LF)120A;UAS:GFP + transgenic zebrafish\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n Collected from 40 adult fish by using + FACS\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ DRS057271\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Danio rerio\n \n + \ \n tissue type\n brain\n + \ \n \n sample + name\n 120A_GFP_plus_Tel\n \n + \ \n genotype\n SAGFF(LF)120A;UAS:GFP\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2018-01-06\n + \ \n \n ENA-LAST-UPDATE\n + \ 2018-01-06\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:08 GMT + - Wed, 17 May 2023 18:37:47 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10225,7 +8459,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -10233,14 +8467,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -10249,7 +8485,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -10261,13 +8497,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:08 GMT + - Wed, 17 May 2023 18:37:48 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10285,7 +8521,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA004272 response: @@ -10319,7 +8555,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:09 GMT + - Wed, 17 May 2023 18:37:48 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10327,68 +8563,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR051062&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIuwrCMBQA0L1fEXDoVLk3iUnT2V0oTm7NiyqhCUlEhPvxesZz4mcYWt3K29J1 - XeGCoDgpqbVRIJUgDjhPgBOoO84LNwuaB0XUoEwIczRSeO2M9oHLaL1F51EgEUkAuh3py8a999JG - Vmru2eXEno1tKeVP8Czmyvr+j2xfwfXhBwAA//8DAKZ6JjGOAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:09 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42B19101B3C510000000000000001.m_1 - NCBI-SID: - - C7A42B19101B3C51_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42B19101B3C51_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:09 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -10399,29 +8573,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=DRR051062&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051062\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051062/DRR051062_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051062/DRR051062_2.fastq.gz\t4036117683;4092158762\t9460e5da5ad16eb9e9dfb035acf4cfac;8ca9d614ef67f2cf435a3afb136d6b89\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051062\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051062/DRR051062_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051062/DRR051062_2.fastq.gz\t4036117683;4092158762\t9460e5da5ad16eb9e9dfb035acf4cfac;8ca9d614ef67f2cf435a3afb136d6b89\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/drr/DRR051/DRR051062\t6477960463\tf17069ee8f943d7c97de24fbdb1cd131\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '468' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:09 GMT + - Wed, 17 May 2023 18:37:49 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -10430,7 +8602,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -10441,7 +8613,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR051063 response: @@ -10475,7 +8647,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:10 GMT + - Wed, 17 May 2023 18:37:48 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10493,7 +8665,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX045955 response: @@ -10550,7 +8722,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:11 GMT + - Wed, 17 May 2023 18:37:49 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10568,57 +8740,44 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS057266 response: body: string: "\n\n\n \n - \ DRS057266\n SAMD00044987\n DRS057266\n \n Danio - rerio\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n DRP003977\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX045955\n \n \n - \ \n \n ENA-RUN\n - \ DRR051063\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA004273\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n description\n - \ These cells are from the same fish as 120A_GFP_plus_Tel\n - \ \n \n bioproject_id\n - \ PRJDB4470\n \n \n - \ sample_name\n 120A_GFP_minus_Tel\n - \ \n \n sample_title\n - \ GFP- cells from telencephalon of SAGFF(LF)120A;UAS:GFP - transgenic zebrafish\n \n \n - \ genotype\n SAGFF(LF)120A;UAS:GFP\n - \ \n \n tissue_type\n - \ brain\n \n \n - \ ENA-SPOT-COUNT\n 76294060\n - \ \n \n ENA-BASE-COUNT\n - \ 15411400120\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-01-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-01-07\n \n + alias=\"SAMD00044987\" center_name=\"National Institute of Genetics (Japan)\" + broker_name=\"DDBJ\">\n \n DRS057266\n + \ SAMD00044987\n + \ \n GFP- cells from telencephalon of SAGFF(LF)120A;UAS:GFP + transgenic zebrafish\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n These cells are from the same fish + as 120A_GFP_plus_Tel\n \n \n + \ \n ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ DRS057266\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Danio rerio\n \n + \ \n tissue type\n brain\n + \ \n \n sample + name\n 120A_GFP_minus_Tel\n \n + \ \n genotype\n SAGFF(LF)120A;UAS:GFP\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2018-01-06\n + \ \n \n ENA-LAST-UPDATE\n + \ 2018-01-06\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:11 GMT + - Wed, 17 May 2023 18:37:49 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10636,7 +8795,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -10644,14 +8803,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -10660,7 +8821,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -10672,13 +8833,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:12 GMT + - Wed, 17 May 2023 18:37:50 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10696,7 +8857,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA004273 response: @@ -10730,7 +8891,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:12 GMT + - Wed, 17 May 2023 18:37:50 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10738,68 +8899,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR051063&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIvQ6CMBQG0J2naOLAhPl6L94Cs7sJcXLjpw2ahhKoMSb34fWM50RnFMc+bO9R - r32Pi4WwWhADTqRmJdimgq0gd7JdjQ7uoQ01AgljYBIgzGgde4Z1TEStd6paA3pb49eUS87bUZpt - TzlNKZrnYYYY08fPJqTd5OUfaXz5KRc/AAAA//8DAK2QvEGPAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:13 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F9C7101B3C910000000000000001.m_1 - NCBI-SID: - - CC95F9C7101B3C91_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F9C7101B3C91_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:13 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -10810,29 +8909,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=DRR051063&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051063\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051063/DRR051063_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051063/DRR051063_2.fastq.gz\t6390520397;6493698053\t9b523f167fd92adc999437039674b9c6;3fb87f88aeaa094df7a854fed0266eb5\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051063\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051063/DRR051063_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051063/DRR051063_2.fastq.gz\t6390520397;6493698053\t9b523f167fd92adc999437039674b9c6;3fb87f88aeaa094df7a854fed0266eb5\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/drr/DRR051/DRR051063\t10230076643\t828606fbf32600fd0973e301732229e7\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '469' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:13 GMT + - Wed, 17 May 2023 18:37:51 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -10841,7 +8938,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -10852,7 +8949,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR051064 response: @@ -10886,7 +8983,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:14 GMT + - Wed, 17 May 2023 18:37:51 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10904,7 +9001,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX045956 response: @@ -10960,7 +9057,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:14 GMT + - Wed, 17 May 2023 18:37:52 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -10978,56 +9075,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS057265 response: body: string: "\n\n\n \n - \ DRS057265\n SAMD00044991\n DRS057265\n \n Danio - rerio\n \n 7955\n Danio + alias=\"SAMD00044991\" center_name=\"National Institute of Genetics (Japan)\" + broker_name=\"DDBJ\">\n \n DRS057265\n + \ SAMD00044991\n + \ \n CS_telencephalon_30 min after TWAA\n + \ \n 7955\n Danio rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n DRP003977\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX045956\n \n \n - \ \n \n ENA-RUN\n - \ DRR051064\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA004274\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n description\n - \ Telencephalon from adult zebrafrish, 30 min after light - stimulation in Two-Way Active Avoidance coditioning\n \n - \ \n bioproject_id\n PRJDB4470\n - \ \n \n sample_name\n - \ CS_Tel_30\n \n \n - \ sample_title\n CS_telencephalon_30 - min after TWAA\n \n \n - \ genotype\n WT\n \n - \ \n tissue_type\n brain\n - \ \n \n ENA-SPOT-COUNT\n - \ 146341598\n \n \n - \ ENA-BASE-COUNT\n 29268319600\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2018-01-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-01-07\n \n + \ \n Telencephalon from adult zebrafrish, + 30 min after light stimulation in Two-Way Active Avoidance coditioning\n + \ \n \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ DRS057265\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Danio rerio\n \n + \ \n tissue type\n brain\n + \ \n \n sample + name\n CS_Tel_30\n \n + \ \n genotype\n WT\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2018-01-06\n + \ \n \n ENA-LAST-UPDATE\n + \ 2018-01-06\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:15 GMT + - Wed, 17 May 2023 18:37:52 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11045,7 +9131,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -11053,14 +9139,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -11069,7 +9157,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -11081,13 +9169,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:15 GMT + - Wed, 17 May 2023 18:37:52 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11105,7 +9193,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA004274 response: @@ -11139,7 +9227,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:17 GMT + - Wed, 17 May 2023 18:37:53 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11147,68 +9235,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR051064&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQqDMBQF0N2vCHRwsty8vJjo3L0gnboZTbElNKIppfA+vm6Hc6Izqn0b10+Q - yzDAarQsBE8EOGJ3WPsGukF7075n6o29i4kdDLMDacvsTQwek7PjTDG0nWERYUCu7/RT9VLKutdq - 3XLJU07quasxpfyNs3rkTZXliBxecSrVHwAA//8DAFGgQi2PAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:17 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95F9F3101B3CD10000000000000001.m_1 - NCBI-SID: - - CC95F9F3101B3CD1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95F9F3101B3CD1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:17 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -11219,29 +9245,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=DRR051064&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051064\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051064/DRR051064_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051064/DRR051064_2.fastq.gz\t13204439635;13344814940\t6e64a0d4d298ff5b1e3c23e8c50f3a60;8d5a6f4e83fa1afdd2276cf0a7124c51\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051064\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051064/DRR051064_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051064/DRR051064_2.fastq.gz\t13204439635;13344814940\t6e64a0d4d298ff5b1e3c23e8c50f3a60;8d5a6f4e83fa1afdd2276cf0a7124c51\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/drr/DRR051/DRR051064\t20822007247\t3e90344702154483eb80c75ad2eb6934\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '471' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:17 GMT + - Wed, 17 May 2023 18:37:54 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -11250,7 +9274,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -11261,7 +9285,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR051065 response: @@ -11295,7 +9319,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:19 GMT + - Wed, 17 May 2023 18:37:54 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11313,7 +9337,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX045957 response: @@ -11369,7 +9393,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:19 GMT + - Wed, 17 May 2023 18:37:54 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11387,57 +9411,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS057272 response: body: string: "\n\n\n \n - \ DRS057272\n SAMD00044990\n DRS057272\n \n Danio - rerio\n \n 7955\n Danio + alias=\"SAMD00044990\" center_name=\"National Institute of Genetics (Japan)\" + broker_name=\"DDBJ\">\n \n DRS057272\n + \ SAMD00044990\n + \ \n CS+US_telencephalon_30 min after TWAA\n + \ \n 7955\n Danio rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n DRP003977\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX045957\n \n \n - \ \n \n ENA-RUN\n - \ DRR051065\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA004275\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n description\n - \ Telencephalon from adult zebrafrish, 30 min after light - and electrical shock association in non-trace Two-Way Active Avoidance conditioning\n - \ \n \n bioproject_id\n - \ PRJDB4470\n \n \n - \ sample_name\n CS+US_Tel_30\n - \ \n \n sample_title\n - \ CS+US_telencephalon_30 min after TWAA\n \n + \ \n Telencephalon from adult zebrafrish, + 30 min after light and electrical shock association in non-trace Two-Way Active + Avoidance conditioning\n \n \n + \ \n ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ DRS057272\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Danio rerio\n \n + \ \n tissue type\n brain\n + \ \n \n sample + name\n CS+US_Tel_30\n \n \ \n genotype\n WT\n - \ \n \n tissue_type\n - \ brain\n \n \n - \ ENA-SPOT-COUNT\n 101196646\n - \ \n \n ENA-BASE-COUNT\n - \ 20239329200\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-01-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-01-07\n \n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2018-01-06\n + \ \n \n ENA-LAST-UPDATE\n + \ 2018-01-06\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:20 GMT + - Wed, 17 May 2023 18:37:54 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11455,7 +9467,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -11463,14 +9475,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -11479,7 +9493,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -11491,13 +9505,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:20 GMT + - Wed, 17 May 2023 18:37:55 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11515,7 +9529,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA004275 response: @@ -11549,7 +9563,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:21 GMT + - Wed, 17 May 2023 18:37:55 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11557,68 +9571,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR051065&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzLuwrCMBQA0L1fEXDoVLk3ryad3YXi5JbElCrBhCQiwv14ux44J36GoVVXPp4u - 6woKQStCKayWMEupiAOaCXACfeO4SL4g3MlsYVYxaOe5scGiF8J75XTUKh7PEpEEoOs7/di4917a - yErNPYec2LMxl1L+xgfbcmV9PyD7Vwx9+AMAAP//AwB4C0SvjwAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:21 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95FC16101B3D110000000000000001.m_1 - NCBI-SID: - - CC95FC16101B3D11_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95FC16101B3D11_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:21 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -11629,29 +9581,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=DRR051065&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051065\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051065/DRR051065_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051065/DRR051065_2.fastq.gz\t9138934313;9235518567\t41d11e8c26bcb996af0be3b8eaa6b82b;21fbcb1077cbdcd82e3b5e81deb3495f\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051065\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051065/DRR051065_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051065/DRR051065_2.fastq.gz\t9138934313;9235518567\t41d11e8c26bcb996af0be3b8eaa6b82b;21fbcb1077cbdcd82e3b5e81deb3495f\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/drr/DRR051/DRR051065\t14396407445\t8fc75ec6ab289c91b33bb5a6e65e4079\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '469' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:22 GMT + - Wed, 17 May 2023 18:37:56 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -11660,7 +9610,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -11671,7 +9621,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR051066 response: @@ -11705,7 +9655,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:23 GMT + - Wed, 17 May 2023 18:37:56 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11723,7 +9673,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX045958 response: @@ -11780,7 +9730,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:23 GMT + - Wed, 17 May 2023 18:37:56 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11798,57 +9748,44 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS057275 response: body: string: "\n\n\n \n - \ DRS057275\n SAMD00044995\n DRS057275\n \n Danio - rerio\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n DRP003977\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX045958\n \n \n - \ \n \n ENA-RUN\n - \ DRR051066\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA004276\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n description\n - \ Collected from 40 adult fish by using FACS\n - \ \n \n bioproject_id\n - \ PRJDB4470\n \n \n - \ sample_name\n h62A_GFP_plus_Tel\n - \ \n \n sample_title\n - \ GFP+ cells from telencephalon of hspGFF62A;UAS:GFP transgenic - zebrafish\n \n \n - \ genotype\n hspGFF62A;UAS:GFP\n - \ \n \n tissue_type\n - \ brain\n \n \n - \ ENA-SPOT-COUNT\n 87106280\n - \ \n \n ENA-BASE-COUNT\n - \ 17421256000\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-01-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-01-07\n \n + alias=\"SAMD00044995\" center_name=\"National Institute of Genetics (Japan)\" + broker_name=\"DDBJ\">\n \n DRS057275\n + \ SAMD00044995\n + \ \n GFP+ cells from telencephalon of hspGFF62A;UAS:GFP + transgenic zebrafish\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n Collected from 40 adult fish by using + FACS\n \n \n \n + \ ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ DRS057275\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Danio rerio\n \n + \ \n tissue type\n brain\n + \ \n \n sample + name\n h62A_GFP_plus_Tel\n \n + \ \n genotype\n hspGFF62A;UAS:GFP\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2018-01-06\n + \ \n \n ENA-LAST-UPDATE\n + \ 2018-01-06\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:24 GMT + - Wed, 17 May 2023 18:37:56 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11866,7 +9803,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -11874,14 +9811,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -11890,7 +9829,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -11902,13 +9841,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:24 GMT + - Wed, 17 May 2023 18:37:58 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11926,7 +9865,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA004276 response: @@ -11958,7 +9897,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:25 GMT + - Wed, 17 May 2023 18:37:58 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -11966,68 +9905,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR051066&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAwzIsQrCMBAA0L1fEXDoVLlLz7Tp7C4UJ7c0SakSeqGJiHAfr298J32Gphwuvxe5 - zjNcEIwRRKIexoFAiwYcO8AOzF3jRDjh+BCiEOJKVvslGBs19R4t2rj2GGwYnIgQgNz29FXtVmsu - rcoHV/ac1LMolxJ/YlArH6pu/+DlFX1tfgAAAP//AwDkc5tCjwAAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:25 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95FD3E101B3D510000000000000001.m_1 - NCBI-SID: - - CC95FD3E101B3D51_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95FD3E101B3D51_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:25 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -12038,29 +9915,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=DRR051066&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051066\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051066/DRR051066_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051066/DRR051066_2.fastq.gz\t7169805313;7221515111\t2e696bf345b14809a945600e21d58496;9d1ad7f4856a258ebb7c5878a96d336a\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051066\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051066/DRR051066_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051066/DRR051066_2.fastq.gz\t7169805313;7221515111\t2e696bf345b14809a945600e21d58496;9d1ad7f4856a258ebb7c5878a96d336a\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/drr/DRR051/DRR051066\t11443087402\t44ddef492cbd69e243c1919ef31d9d7a\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '469' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:26 GMT + - Wed, 17 May 2023 18:37:59 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -12069,7 +9944,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -12080,7 +9955,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR051067 response: @@ -12114,7 +9989,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:27 GMT + - Wed, 17 May 2023 18:37:59 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12132,7 +10007,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX045959 response: @@ -12189,7 +10064,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:28 GMT + - Wed, 17 May 2023 18:37:59 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12207,57 +10082,44 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS057267 response: body: string: "\n\n\n \n - \ DRS057267\n SAMD00044994\n DRS057267\n \n Danio - rerio\n \n 7955\n Danio - rerio\n zebrafish\n - \ \n \n \n \n - \ ENA-STUDY\n DRP003977\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ DRX045959\n \n \n - \ \n \n ENA-RUN\n - \ DRR051067\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA004277\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n description\n - \ These cells are from the same fish as h62A_GFP_plus_Tel\n - \ \n \n bioproject_id\n - \ PRJDB4470\n \n \n - \ sample_name\n h62A_GFP_minus_Tel\n - \ \n \n sample_title\n - \ GFP- cells from telencephalon of hspGFF62A;UAS:GFP transgenic - zebrafish\n \n \n - \ genotype\n hspGFF62A;UAS:GFP\n - \ \n \n tissue_type\n - \ brain\n \n \n - \ ENA-SPOT-COUNT\n 89569368\n - \ \n \n ENA-BASE-COUNT\n - \ 17913873600\n \n - \ \n ENA-FIRST-PUBLIC\n - \ 2018-01-07\n \n - \ \n ENA-LAST-UPDATE\n - \ 2018-01-07\n \n + alias=\"SAMD00044994\" center_name=\"National Institute of Genetics (Japan)\" + broker_name=\"DDBJ\">\n \n DRS057267\n + \ SAMD00044994\n + \ \n GFP- cells from telencephalon of hspGFF62A;UAS:GFP + transgenic zebrafish\n \n 7955\n + \ Danio rerio\n zebrafish\n + \ \n These cells are from the same fish + as h62A_GFP_plus_Tel\n \n \n + \ \n ENA-FASTQ-FILES\n \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ DRS057267\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n organism\n + \ Danio rerio\n \n + \ \n tissue type\n brain\n + \ \n \n sample + name\n h62A_GFP_minus_Tel\n \n + \ \n genotype\n hspGFF62A;UAS:GFP\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2018-01-06\n + \ \n \n ENA-LAST-UPDATE\n + \ 2018-01-06\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:28 GMT + - Wed, 17 May 2023 18:38:00 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12275,7 +10137,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRP003977 response: @@ -12283,14 +10145,16 @@ interactions: string: "\n\n\n \n \ DRP003977\n PRJDB4470\n - \ PRJDB4470\n + \ PRJDB4470\n \ DRP003977\n \n \ \n Gene expression analysis of the - zebrafish brain\n \n Gene expression profiling by RNA-seq - of specific regions and subpopulations of neurons in the zebrafish brain that - control behaviors.\n \n \n - \ \n \n ENA-SAMPLE\n + zebrafish brain\n \n + \ Gene expression profiling by RNA-seq of specific + regions and subpopulations of neurons in the zebrafish brain that control + behaviors.\n Gene expression + profiling by RNA-seq of specific regions and subpopulations of neurons in + the zebrafish brain that control behaviors.\n \n + \ \n \n \n ENA-SAMPLE\n \ DRS057265-DRS057267,DRS057269-DRS057272,DRS057274-DRS057275\n \ \n \n \n \ \n ENA-EXPERIMENT\n @@ -12299,7 +10163,7 @@ interactions: \ ENA-RUN\n DRR051059-DRR051067\n \ \n \n \n \ \n ENA-SUBMISSION\n - \ DRA004276\n \n \n + \ DRA008860\n \n \n \ \n \n ENA-FASTQ-FILES\n \ \n \ \n \n \n @@ -12311,13 +10175,13 @@ interactions: \ ENA-BASE-COUNT\n 182690550386\n \ \n \n ENA-FIRST-PUBLIC\n \ 2018-01-08\n \n \n - \ ENA-LAST-UPDATE\n 2018-01-08\n + \ ENA-LAST-UPDATE\n 2021-08-11\n \ \n \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:28 GMT + - Wed, 17 May 2023 18:37:59 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12335,7 +10199,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRA004277 response: @@ -12369,7 +10233,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:29 GMT + - Wed, 17 May 2023 18:38:00 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -12377,68 +10241,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=DRR051067&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '43' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAAxTKsQrCMBQF0L1fEXDoVLkvTeJLZ3ehOLk1bUqVYEobEeF9vLoezkEfUe3bsL6C - nPseluBOQmTJcWvBJBrEDaiBuxJ3LXeabhIm43TU3pLBv3KY2fuRCD5iMhARA8jlmT6qXkpZ91qt - Wy55zEnddzWklN9xUnPeVFl+kMMjjqX6AgAA//8DAAVtKA+PAAAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:30 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95FD87101B3DA10000000000000001.m_1 - NCBI-SID: - - CC95FD87101B3DA1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95FD87101B3DA1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:30 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -12449,29 +10251,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=DRR051067&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051067\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051067/DRR051067_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051067/DRR051067_2.fastq.gz\t7040712780;7471523344\t83884a210f90531fc75f3e5751f93159;aba57b33a49964ab4b811b71e5827cde\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nDRR051067\tftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051067/DRR051067_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/DRR051/DRR051067/DRR051067_2.fastq.gz\t7040712780;7471523344\t83884a210f90531fc75f3e5751f93159;aba57b33a49964ab4b811b71e5827cde\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/drr/DRR051/DRR051067\t11516835081\tbd462e29514051688bf899c1109e0d40\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '469' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:31 GMT + - Wed, 17 May 2023 18:38:01 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -12480,5 +10280,5 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' version: 1 diff --git a/test_volume/cassettes/surveyor.sra.survey_nonexistant.yaml b/test_volume/cassettes/surveyor.sra.survey_nonexistant.yaml index c6b549f81..a3e30fdf2 100644 --- a/test_volume/cassettes/surveyor.sra.survey_nonexistant.yaml +++ b/test_volume/cassettes/surveyor.sra.survey_nonexistant.yaml @@ -9,19 +9,19 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/ERP006216 response: body: - string: "\n\n 1627501393717\n + string: "\n\n 1684348606015\n \ 404\n Not Found\n ERP006216 not found.\n /ena/browser/api/xml/ERP006216\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:43:13 GMT + - Wed, 17 May 2023 18:36:45 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: diff --git a/test_volume/cassettes/surveyor.sra.survey_unmated_reads.yaml b/test_volume/cassettes/surveyor.sra.survey_unmated_reads.yaml index 7590ed1bd..178edaa76 100644 --- a/test_volume/cassettes/surveyor.sra.survey_unmated_reads.yaml +++ b/test_volume/cassettes/surveyor.sra.survey_unmated_reads.yaml @@ -9,7 +9,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -56,7 +56,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:36 GMT + - Wed, 17 May 2023 18:38:04 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -74,7 +74,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603661 response: @@ -121,7 +121,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:36 GMT + - Wed, 17 May 2023 18:38:04 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -139,7 +139,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725593 response: @@ -198,7 +198,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:36 GMT + - Wed, 17 May 2023 18:38:04 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -216,52 +216,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716925 response: body: string: "\n\n\n \n - \ SRS716925\n SAMN03097499\n - \ GSM1519616\n \n + alias=\"GSM1519616\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716925\n SAMN03097499\n GSM1519616\n \n \ RNA-seq of the corpus callosum #5308\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725593\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603661\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n autism spectrum disorder\n - \ \n \n ENA-SPOT-COUNT\n - \ 162048745\n \n \n - \ ENA-BASE-COUNT\n 28185753687\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716925\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ autism spectrum disorder\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:37 GMT + - Wed, 17 May 2023 18:38:06 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -279,7 +272,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -326,7 +319,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:38 GMT + - Wed, 17 May 2023 18:38:05 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -344,7 +337,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -381,7 +374,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:38 GMT + - Wed, 17 May 2023 18:38:06 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -389,69 +382,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603661&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOPW4CMRBA4T6nsJSCCjQztseevUQkoKLzX7SJVni164CQfHiWtOnSvuZ973SA - t3UJ80/sp+MRGTQzdiRgzYJaXCdAs0fYA59JD2AGlEv3kiGzIbEB2LroEbyQ9sgxOGdT790A9I/r - 9FC7sbV53al5qa2mOqmvVYVpqveS1WddVBu3UON3Se0v5XDLMYU0ls2ktx8Q/pLci0T6DDIYGIy5 - dJJkMZBx2z+yzt4iFh9LBm+oFPkv6QkAAP//AwBM6dz2IwEAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:39 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42D50101B3E310000000000000001.m_1 - NCBI-SID: - - C7A42D50101B3E31_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42D50101B3E31_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:39 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -462,7 +392,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&id=25549968&retmode=json&tool=refinebio&email=hello@refine.bio response: @@ -472,18 +402,18 @@ interactions: vR9vZmZUTAMLNjOG6cTYIPs4jiQTVvjJ4rWzSawXK/yqUJJpQg5HPXhs3nC52ay329s7fK9+5x8/ YVelwlM8DhRywbK+WcMD97Cqk87/Gd5F6bPcOgPd5APca2eSQTEoJ7l9RktjjrxoeL44lyt251DS ehN9YDmvk064Ip3S0BYyLcGhEDkoctAVQo/Rf2jpFVvYl/bphNrib7zKkSzsCqknMmakieVv3zcA - 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'*' @@ -498,18 +428,20 @@ interactions: Content-Type: - application/json; charset=UTF-8 Date: - - Wed, 28 Jul 2021 19:45:38 GMT + - Wed, 17 May 2023 18:38:07 GMT Keep-Alive: - timeout=4, max=40 NCBI-PHID: - - 322C6078A1DD3885000034FA20798E62.1.1.m_1 + - 322C36E9C9D0D90500005E0AE30E5C73.1.1.m_1 NCBI-SID: - - 9F4B36537CC83871_AAECSID + - 9A764307C0400C6F_B567SID + Referrer-Policy: + - origin-when-cross-origin Server: - Finatra Set-Cookie: - - ncbi_sid=9F4B36537CC83871_AAECSID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:39 GMT + - ncbi_sid=9A764307C0400C6F_B567SID; domain=.nih.gov; path=/; expires=Fri, 17 + May 2024 18:38:07 GMT Strict-Transport-Security: - max-age=31536000; includeSubDomains; preload Transfer-Encoding: @@ -537,29 +469,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603661&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603661\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603661/SRR1603661.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603661/SRR1603661_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603661/SRR1603661_2.fastq.gz\t852745978;6751980628;6949912932\tbbd8e04db475a6b28fed8c74eeb9d701;502a9a482bfa5aa75865ccc0105ad13c;fffd24457418d255991f54ec82a39d57\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603661\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603661/SRR1603661.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603661/SRR1603661_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603661/SRR1603661_2.fastq.gz\t852745978;6751980628;6949912932\tbbd8e04db475a6b28fed8c74eeb9d701;502a9a482bfa5aa75865ccc0105ad13c;fffd24457418d255991f54ec82a39d57\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/001/SRR1603661\t12063691397\t89d0d64295a0657b8108923816ba775c\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:39 GMT + - Wed, 17 May 2023 18:38:07 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -568,7 +498,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -579,7 +509,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603662 response: @@ -626,7 +556,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:40 GMT + - Wed, 17 May 2023 18:38:07 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -644,7 +574,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725594 response: @@ -703,7 +633,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:41 GMT + - Wed, 17 May 2023 18:38:08 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -721,52 +651,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716926 response: body: string: "\n\n\n \n - \ SRS716926\n SAMN03097500\n - \ GSM1519617\n \n + alias=\"GSM1519617\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716926\n SAMN03097500\n GSM1519617\n \n \ RNA-seq of the corpus callosum #5144\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725594\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603662\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n autism spectrum disorder\n - \ \n \n ENA-SPOT-COUNT\n - \ 172822773\n \n \n - \ ENA-BASE-COUNT\n 29944432518\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716926\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ autism spectrum disorder\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:41 GMT + - Wed, 17 May 2023 18:38:08 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -784,7 +707,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -831,7 +754,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:42 GMT + - Wed, 17 May 2023 18:38:09 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -849,7 +772,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -886,7 +809,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:42 GMT + - Wed, 17 May 2023 18:38:08 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -894,69 +817,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603662&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - 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SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -967,29 +827,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603662&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603662\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603662/SRR1603662.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603662/SRR1603662_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603662/SRR1603662_2.fastq.gz\t998808350;7340614837;7522532959\t66f002c1cee8243129e9e73aa66a4e49;d9c461cdf14c6077f56b722fca8b6920;95eb1c28105297e66a9d70996ae69634\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603662\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603662/SRR1603662.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603662/SRR1603662_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603662/SRR1603662_2.fastq.gz\t998808350;7340614837;7522532959\t66f002c1cee8243129e9e73aa66a4e49;d9c461cdf14c6077f56b722fca8b6920;95eb1c28105297e66a9d70996ae69634\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/002/SRR1603662\t13229044906\tc439617cdebf8270d8ce75a9f8083d50\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:43 GMT + - Wed, 17 May 2023 18:38:10 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -998,7 +856,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -1009,7 +867,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603663 response: @@ -1056,7 +914,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:44 GMT + - Wed, 17 May 2023 18:38:10 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1074,7 +932,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725595 response: @@ -1133,7 +991,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:44 GMT + - Wed, 17 May 2023 18:38:10 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1151,52 +1009,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716927 response: body: string: "\n\n\n \n - \ SRS716927\n SAMN03097501\n - \ GSM1519618\n \n + alias=\"GSM1519618\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716927\n SAMN03097501\n GSM1519618\n \n \ RNA-seq of the corpus callosum #4727\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725595\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603663\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n not available\n - \ \n \n ENA-SPOT-COUNT\n - \ 143636433\n \n \n - \ ENA-BASE-COUNT\n 25287600247\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716927\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ not available\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:45 GMT + - Wed, 17 May 2023 18:38:11 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1214,7 +1065,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -1261,7 +1112,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:45 GMT + - Wed, 17 May 2023 18:38:11 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1279,7 +1130,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -1316,7 +1167,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:45 GMT + - Wed, 17 May 2023 18:38:11 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1324,69 +1175,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603663&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOPU7EMBBA4Z5TWKLYKqv5sx3nEkgL1XaxJ1ZA0TpKAghpDs8iSjrap1d8j3SG - h30b1/dsz5cLBuAQ2JKPfe8xsRgBSofQQXghGigNAleTWEuWHJglBcIU5L6SV+w1VNZiZgJgT7fl - y53m41j3k1u3drTSFve6u3FZ2uekrrbNHfM9tPw2leOv5PyhuYxlngwxEETxyD+k2EHq8JckA/ZX - q1STRi3I1TN7GTXARMqZUWtM/r+kbwAAAP//AwCIsZKKIgEAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:46 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95FED7101B3EA10000000000000001.m_1 - NCBI-SID: - - CC95FED7101B3EA1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95FED7101B3EA1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:46 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -1397,29 +1185,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603663&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603663\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/003/SRR1603663/SRR1603663.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/003/SRR1603663/SRR1603663_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/003/SRR1603663/SRR1603663_2.fastq.gz\t490810289;5442785670;5470533592\t27f4fe0eb14986a1727f080bc6db4d2b;83becc171cdf03d7c25aaf1d798b66e2;af9417bfd987797f395d201176adf9b3\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603663\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/003/SRR1603663/SRR1603663.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/003/SRR1603663/SRR1603663_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/003/SRR1603663/SRR1603663_2.fastq.gz\t490810289;5442785670;5470533592\t27f4fe0eb14986a1727f080bc6db4d2b;83becc171cdf03d7c25aaf1d798b66e2;af9417bfd987797f395d201176adf9b3\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/003/SRR1603663\t9578851934\t47fcb4b6334962196493425d18d6f3dc\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '600' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:47 GMT + - Wed, 17 May 2023 18:38:12 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -1428,7 +1214,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -1439,7 +1225,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603664 response: @@ -1486,7 +1272,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:48 GMT + - Wed, 17 May 2023 18:38:12 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1504,7 +1290,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725596 response: @@ -1563,7 +1349,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:49 GMT + - Wed, 17 May 2023 18:38:13 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1581,52 +1367,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716928 response: body: string: "\n\n\n \n - \ SRS716928\n SAMN03097502\n - \ GSM1519619\n \n + alias=\"GSM1519619\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716928\n SAMN03097502\n GSM1519619\n \n \ RNA-seq of the corpus callosum #5163\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725596\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603664\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n not available\n - \ \n \n ENA-SPOT-COUNT\n - \ 163384856\n \n \n - \ ENA-BASE-COUNT\n 28647959886\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716928\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ not available\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:49 GMT + - Wed, 17 May 2023 18:38:13 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1644,7 +1423,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -1691,7 +1470,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:50 GMT + - Wed, 17 May 2023 18:38:13 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1709,7 +1488,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -1746,7 +1525,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:50 GMT + - Wed, 17 May 2023 18:38:13 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1754,69 +1533,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603664&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOvU7DMBSG4Z2rsMTQqdV3fPybm0Aqnbo5tqOAIhwlBoR0Lp50ZkN6p3d6nvUF - T/uW1s9RXq9XcmDnjBCxjWRitF40yJwJZ7ib5oGOcJdpQo3sCudcUTBB52QQTNIUIrETEQPIy8fy - o05z7+t+UuvWesttUW+7SsvSvmtRU9tUn4/Rxvea+1/K5auMOeW5CkFHC8+MB8k/SJpviIMNA9xd - kFCt86FaMkUHNhxGU/zhjNmzKf8l/QIAAP//AwD/hMbsIwEAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:51 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95FF33101B3EF10000000000000001.m_1 - NCBI-SID: - - CC95FF33101B3EF1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95FF33101B3EF1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:51 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -1827,29 +1543,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603664&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603664\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/004/SRR1603664/SRR1603664.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/004/SRR1603664/SRR1603664_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/004/SRR1603664/SRR1603664_2.fastq.gz\t663856366;6479922904;6586111087\td79a415b33c6fbd2159ea21122539fd8;a7337c4c402bff8aaa6dd474d7743a16;51d4b7a03a427f94df589dfb4060f4cb\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603664\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/004/SRR1603664/SRR1603664.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/004/SRR1603664/SRR1603664_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/004/SRR1603664/SRR1603664_2.fastq.gz\t663856366;6479922904;6586111087\td79a415b33c6fbd2159ea21122539fd8;a7337c4c402bff8aaa6dd474d7743a16;51d4b7a03a427f94df589dfb4060f4cb\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/004/SRR1603664\t11359149957\tff0e936d3cce0d0f02ca4084a2189136\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:52 GMT + - Wed, 17 May 2023 18:38:14 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -1858,7 +1572,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -1869,7 +1583,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603665 response: @@ -1916,7 +1630,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:52 GMT + - Wed, 17 May 2023 18:38:15 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -1934,7 +1648,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725597 response: @@ -1993,7 +1707,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:53 GMT + - Wed, 17 May 2023 18:38:15 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2011,52 +1725,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716929 response: body: string: "\n\n\n \n - \ SRS716929\n SAMN03097503\n - \ GSM1519620\n \n + alias=\"GSM1519620\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716929\n SAMN03097503\n GSM1519620\n \n \ RNA-seq of the corpus callosum #5391\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725597\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603665\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n not available\n - \ \n \n ENA-SPOT-COUNT\n - \ 155224100\n \n \n - \ ENA-BASE-COUNT\n 27186376672\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716929\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ not available\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:53 GMT + - Wed, 17 May 2023 18:38:16 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2074,7 +1781,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -2121,7 +1828,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:53 GMT + - Wed, 17 May 2023 18:38:16 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2139,7 +1846,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -2176,7 +1883,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:54 GMT + - Wed, 17 May 2023 18:38:16 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2184,69 +1891,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603665&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOO27DMAwA0L2nENAhUwJS1I++RIE0UzZRluAWRmTYboICPHw/a7eub3rP9gRP - 25qXD9HX8xkDUAheEXxCZo7eqQV0R4QjhIulAWAAf9UWEQUSs8NQGUMZsWUQjJQAhUhVHYC+3OZP - c5j2fdkOZln73kufzdtm8jz3Rx1N66vZp2/o8l7L/rdyuo9ScpmqJk6IyabfUfwZWboAD+QHxKuC - K7kJ+SaMLZbKWaoD8X6slBqV/46+AAAA//8DAPEhuy4iAQAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:55 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95FF99101B3F310000000000000001.m_1 - NCBI-SID: - - CC95FF99101B3F31_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95FF99101B3F31_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:55 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -2257,29 +1901,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603665&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603665\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/005/SRR1603665/SRR1603665.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/005/SRR1603665/SRR1603665_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/005/SRR1603665/SRR1603665_2.fastq.gz\t637411585;6019011707;6096423865\tabe09a733a7f7164685e6a55c1c48e2a;0673ce2bd91a7bb8b7058af295715391;553f4ff4018716dc9fdffa9ff8cfdaa6\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603665\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/005/SRR1603665/SRR1603665.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/005/SRR1603665/SRR1603665_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/005/SRR1603665/SRR1603665_2.fastq.gz\t637411585;6019011707;6096423865\tabe09a733a7f7164685e6a55c1c48e2a;0673ce2bd91a7bb8b7058af295715391;553f4ff4018716dc9fdffa9ff8cfdaa6\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/005/SRR1603665\t10581999754\tf711b0899416e916cd1fa0b173801b33\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:45:56 GMT + - Wed, 17 May 2023 18:38:17 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -2288,7 +1930,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -2299,7 +1941,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603666 response: @@ -2346,7 +1988,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:56 GMT + - Wed, 17 May 2023 18:38:17 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2364,7 +2006,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725598 response: @@ -2423,7 +2065,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:57 GMT + - Wed, 17 May 2023 18:38:17 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2441,52 +2083,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716930 response: body: string: "\n\n\n \n - \ SRS716930\n SAMN03097504\n - \ GSM1519621\n \n + alias=\"GSM1519621\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716930\n SAMN03097504\n GSM1519621\n \n \ RNA-seq of the corpus callosum #5242\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725598\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603666\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n not available\n - \ \n \n ENA-SPOT-COUNT\n - \ 163481475\n \n \n - \ ENA-BASE-COUNT\n 28665079898\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716930\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ not available\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:57 GMT + - Wed, 17 May 2023 18:38:18 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2504,7 +2139,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -2551,7 +2186,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:58 GMT + - Wed, 17 May 2023 18:38:18 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2569,7 +2204,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -2606,7 +2241,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:45:58 GMT + - Wed, 17 May 2023 18:38:19 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2614,69 +2249,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603666&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOu07DMBQA0J2vsMTQqdV92L52fgKpdOpmOzcKKKqjxLRC8sdDZzbWM51XOsHL - vqX1K/f38xk9sPe+IzorjJEIOwHaI8IR5AI4WDcgX3tgTJTGoFbEKaBkP3GIGlEZiLT3bgH62235 - Noe5tXU/mHWrrZa6mI/dpGWpDx3NVDfT5l+o+VNL+1s53cdcUpm1o3MYMQjEZ0meJeILwkB2cOHa - J7JlkiLFUWSIScakQEEhk+XI/r+lHwAAAP//AwDIQTI1IwEAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:45:59 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42EE4101B3F710000000000000001.m_1 - NCBI-SID: - - C7A42EE4101B3F71_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42EE4101B3F71_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:45:59 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -2687,29 +2259,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603666&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603666\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/006/SRR1603666/SRR1603666.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/006/SRR1603666/SRR1603666_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/006/SRR1603666/SRR1603666_2.fastq.gz\t653021683;6452705895;6593977478\td9eb9f296bb1ed25506469e9ba292272;8730290dbb90c069cac0662d3b06a69f;5a32c8ae794dd25e5d1e85c89a751020\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603666\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/006/SRR1603666/SRR1603666.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/006/SRR1603666/SRR1603666_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/006/SRR1603666/SRR1603666_2.fastq.gz\t653021683;6452705895;6593977478\td9eb9f296bb1ed25506469e9ba292272;8730290dbb90c069cac0662d3b06a69f;5a32c8ae794dd25e5d1e85c89a751020\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/006/SRR1603666\t11547319221\t831a2ad8e4775e017b6f389e91e3022e\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:46:00 GMT + - Wed, 17 May 2023 18:38:19 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -2718,7 +2288,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -2729,7 +2299,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603667 response: @@ -2776,7 +2346,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:00 GMT + - Wed, 17 May 2023 18:38:20 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2794,7 +2364,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725599 response: @@ -2853,7 +2423,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:01 GMT + - Wed, 17 May 2023 18:38:20 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2871,52 +2441,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716931 response: body: string: "\n\n\n \n - \ SRS716931\n SAMN03097505\n - \ GSM1519622\n \n + alias=\"GSM1519622\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716931\n SAMN03097505\n GSM1519622\n \n \ RNA-seq of the corpus callosum #4899\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725599\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603667\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n autism spectrum disorder\n - \ \n \n ENA-SPOT-COUNT\n - \ 167246977\n \n \n - \ ENA-BASE-COUNT\n 29215101734\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716931\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ autism spectrum disorder\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:01 GMT + - Wed, 17 May 2023 18:38:20 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2934,7 +2497,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -2981,7 +2544,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:02 GMT + - Wed, 17 May 2023 18:38:21 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -2999,7 +2562,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -3036,7 +2599,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:02 GMT + - Wed, 17 May 2023 18:38:21 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3044,69 +2607,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603667&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOsWoDMRAE0D5fIUjhyma1klbS/UTAceVOWum4hCM67pQEw368z7U7VwMDw7x3 - PMHbtqblN8vn+awJDJEXrWPAgEjBCoK2Rw1H8BeAwcAA7irAMXNxbD2jo2qcTdHFTHsUcsmJiAWQ - j5/5pg5T78t2UMvaeuM2q69NpXlu/7Wosa2qT3vR8nfl/kw5/ZXMiacq2lq7f4A2D5J/kNBcNAzO - D4hXGSmSqfuIS8aRjC+1FOMhUQ0pcHqVdAcAAP//AwC3OmTcIwEAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:46:03 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42F75101B3FB10000000000000001.m_1 - NCBI-SID: - - C7A42F75101B3FB1_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42F75101B3FB1_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:46:03 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -3117,29 +2617,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603667&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603667\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/007/SRR1603667/SRR1603667.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/007/SRR1603667/SRR1603667_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/007/SRR1603667/SRR1603667_2.fastq.gz\t760314180;6805213530;6809395591\t658f2f20dcd16b96c7ff7e9d7da63635;333a0f373e628864dc005ab47e5c241c;b4e6c453e6d964c33ec48adfdce22900\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603667\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/007/SRR1603667/SRR1603667.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/007/SRR1603667/SRR1603667_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/007/SRR1603667/SRR1603667_2.fastq.gz\t760314180;6805213530;6809395591\t658f2f20dcd16b96c7ff7e9d7da63635;333a0f373e628864dc005ab47e5c241c;b4e6c453e6d964c33ec48adfdce22900\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/007/SRR1603667\t11982822684\t0c9bcd5c47c256e354a959b64a9d65a5\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:46:04 GMT + - Wed, 17 May 2023 18:38:21 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -3148,7 +2646,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -3159,7 +2657,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603668 response: @@ -3206,7 +2704,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:04 GMT + - Wed, 17 May 2023 18:38:22 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3224,7 +2722,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725600 response: @@ -3283,7 +2781,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:05 GMT + - Wed, 17 May 2023 18:38:22 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3301,52 +2799,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716934 response: body: string: "\n\n\n \n - \ SRS716934\n SAMN03097506\n - \ GSM1519623\n \n + alias=\"GSM1519623\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716934\n SAMN03097506\n GSM1519623\n \n \ RNA-seq of the corpus callosum #4670\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725600\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603668\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n not available\n - \ \n \n ENA-SPOT-COUNT\n - \ 166075695\n \n \n - \ ENA-BASE-COUNT\n 29040785228\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716934\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ not available\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:06 GMT + - Wed, 17 May 2023 18:38:23 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3364,7 +2855,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -3411,7 +2902,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:07 GMT + - Wed, 17 May 2023 18:38:23 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3429,7 +2920,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -3466,7 +2957,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:07 GMT + - Wed, 17 May 2023 18:38:23 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3474,69 +2965,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603668&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOPWrEMBBA4T6nEKTYapeZ0e/4EoHNVulGIxsnmJWxlYSADh9vnS7tgwffM13g - ad9k/cz99XrFADaE1BGZMLH1CToBujPCGcKN7ABhcPzWUbX4PNExFBHK4qx3XEiz2JAT9t4dQH+5 - Lz/mNLe27iezbrVVrYt5340sS/0ei5nqZtp8hJo/Rm1/KZevklV0HjtaCMw+xvggxQeJ7A148GHA - g1S8jEFsUY0eo5uIrePEGVUmSUj/Jf0CAAD//wMAMjlgZiMBAAA= - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:46:08 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A42FD2101B40010000000000000001.m_1 - NCBI-SID: - - C7A42FD2101B4001_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A42FD2101B4001_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:46:08 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -3547,29 +2975,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603668&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603668\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/008/SRR1603668/SRR1603668.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/008/SRR1603668/SRR1603668_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/008/SRR1603668/SRR1603668_2.fastq.gz\t746191256;6748922507;6789323499\t1929c6ddedde0bccd594d572548529fd;7859ec8ac9d10a54e6f9ae1249ac050a;38c8883b06c88930a116c314ea738bb1\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603668\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/008/SRR1603668/SRR1603668.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/008/SRR1603668/SRR1603668_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/008/SRR1603668/SRR1603668_2.fastq.gz\t746191256;6748922507;6789323499\t1929c6ddedde0bccd594d572548529fd;7859ec8ac9d10a54e6f9ae1249ac050a;38c8883b06c88930a116c314ea738bb1\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/008/SRR1603668\t11921893580\t1ccd5bf2160daa2ba43549d2cba36b81\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:46:08 GMT + - Wed, 17 May 2023 18:38:24 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -3578,7 +3004,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -3589,7 +3015,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603669 response: @@ -3636,7 +3062,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:09 GMT + - Wed, 17 May 2023 18:38:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3654,7 +3080,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725601 response: @@ -3713,7 +3139,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:09 GMT + - Wed, 17 May 2023 18:38:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3731,52 +3157,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716932 response: body: string: "\n\n\n \n - \ SRS716932\n SAMN03097507\n - \ GSM1519624\n \n + alias=\"GSM1519624\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716932\n SAMN03097507\n GSM1519624\n \n \ RNA-seq of the corpus callosum #5407\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725601\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603669\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n not available\n - \ \n \n ENA-SPOT-COUNT\n - \ 165948423\n \n \n - \ ENA-BASE-COUNT\n 28994442750\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716932\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ not available\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:10 GMT + - Wed, 17 May 2023 18:38:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3794,7 +3213,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -3841,7 +3260,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:10 GMT + - Wed, 17 May 2023 18:38:25 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3859,7 +3278,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -3896,7 +3315,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:11 GMT + - Wed, 17 May 2023 18:38:26 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -3904,69 +3323,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603669&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOu07DQBCF4Z6nWIkiVaKZ2fFe/BJIIVW62YtlkMVa9gJCmofHqelojn6d6num - Czztm6yfSV+vV3RgnYuKGI4EP/ioBMhnhDO4G9FoccThrrUMkSZiF10KkaWwl1wkO8w14kSqygD6 - 8rH8mNPc+7qfzLq13nJbzNtuZFnady1mapvp83G09F5z/0u5fJWUJc9V0QZmT8HCg+QfJLI3CCPT - iHRXyM6zSArABTyRq8JWxOKxrmb5L+kXAAD//wMABzwKSCMBAAA= - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:46:12 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC9501E2101B40410000000000000001.m_1 - NCBI-SID: - - CC9501E2101B4041_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC9501E2101B4041_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:46:12 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -3977,29 +3333,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603669&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603669\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/009/SRR1603669/SRR1603669.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/009/SRR1603669/SRR1603669_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/009/SRR1603669/SRR1603669_2.fastq.gz\t740881940;6633314923;6798374768\te796f40ceb70b3cd1dc0a9a542859b48;aa894e681310d52d109c9cd827e2bbc8;963d262188a629d68131281893e0f569\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603669\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/009/SRR1603669/SRR1603669.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/009/SRR1603669/SRR1603669_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/009/SRR1603669/SRR1603669_2.fastq.gz\t740881940;6633314923;6798374768\te796f40ceb70b3cd1dc0a9a542859b48;aa894e681310d52d109c9cd827e2bbc8;963d262188a629d68131281893e0f569\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/009/SRR1603669\t11816007579\ted592f24696b894ad47acdac61ce91f2\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:46:12 GMT + - Wed, 17 May 2023 18:38:27 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -4008,7 +3362,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -4019,7 +3373,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603670 response: @@ -4066,7 +3420,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:13 GMT + - Wed, 17 May 2023 18:38:27 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4084,7 +3438,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725602 response: @@ -4143,7 +3497,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:14 GMT + - Wed, 17 May 2023 18:38:27 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4161,52 +3515,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716933 response: body: string: "\n\n\n \n - \ SRS716933\n SAMN03097508\n - \ GSM1519625\n \n + alias=\"GSM1519625\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716933\n SAMN03097508\n GSM1519625\n \n \ RNA-seq of the corpus callosum #4999\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725602\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603670\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n autism spectrum disorder\n - \ \n \n ENA-SPOT-COUNT\n - \ 176741134\n \n \n - \ ENA-BASE-COUNT\n 30790991868\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716933\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ autism spectrum disorder\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:13 GMT + - Wed, 17 May 2023 18:38:27 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4224,7 +3571,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -4271,7 +3618,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:14 GMT + - Wed, 17 May 2023 18:38:28 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4289,7 +3636,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -4326,7 +3673,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:14 GMT + - Wed, 17 May 2023 18:38:28 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4334,69 +3681,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603670&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOvW7CMBRA4b1PYakDE+j+2L5OXqISZWKzHUcBRXWUGCqk+/DA3K3b0Zm+TzrA - x7bG5Zb0+3hED+wFFBmI0TO/mgDtHmEP/kTUM/coZ3URaHAudJGAkhCDjN6KZZd8GaJTVQugXz/z - w+ym1pZtZ5a1tprrbC6bifNcf8tgxrqaNr1GTdeS21/K4T6kHPNUFB1KoK4TfpPkTSI+Qehd6AnP - WgJ6zGUsriSUhBTGAC5TROtTkn+TngAAAP//AwC/Hb8rIwEAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:46:15 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95022E101B40810000000000000001.m_1 - NCBI-SID: - - CC95022E101B4081_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95022E101B4081_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:46:16 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -4407,29 +3691,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603670&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603670\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/000/SRR1603670/SRR1603670.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/000/SRR1603670/SRR1603670_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/000/SRR1603670/SRR1603670_2.fastq.gz\t867311136;7337073265;7405687750\t2b6346b111fd7b6767b5703771c05312;0cf4bd30cd674d940b5ecb1b816c2e6f;4f1f26d76abae3ef698f4c552456a54d\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603670\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/000/SRR1603670/SRR1603670.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/000/SRR1603670/SRR1603670_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/000/SRR1603670/SRR1603670_2.fastq.gz\t867311136;7337073265;7405687750\t2b6346b111fd7b6767b5703771c05312;0cf4bd30cd674d940b5ecb1b816c2e6f;4f1f26d76abae3ef698f4c552456a54d\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/000/SRR1603670\t13023163370\t5a02d5589a202b72307f647435b6eda5\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:46:21 GMT + - Wed, 17 May 2023 18:38:29 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -4438,7 +3720,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -4449,7 +3731,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603671 response: @@ -4496,7 +3778,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:21 GMT + - Wed, 17 May 2023 18:38:29 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4514,7 +3796,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725603 response: @@ -4573,7 +3855,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:22 GMT + - Wed, 17 May 2023 18:38:29 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4591,52 +3873,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716935 response: body: string: "\n\n\n \n - \ SRS716935\n SAMN03097509\n - \ GSM1519626\n \n + alias=\"GSM1519626\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716935\n SAMN03097509\n GSM1519626\n \n \ RNA-seq of the corpus callosum #5302\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725603\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603671\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n autism spectrum disorder\n - \ \n \n ENA-SPOT-COUNT\n - \ 160334858\n \n \n - \ ENA-BASE-COUNT\n 27850705155\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716935\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ autism spectrum disorder\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:22 GMT + - Wed, 17 May 2023 18:38:30 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4654,7 +3929,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -4701,7 +3976,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:24 GMT + - Wed, 17 May 2023 18:38:30 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4719,7 +3994,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -4756,7 +4031,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:24 GMT + - Wed, 17 May 2023 18:38:30 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4764,69 +4039,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603671&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOu27DMAyF4b1PIaBDpgQkRUuKX6JAmimbLhTcwqgMW0lRgA9fZ+7W7eAfDr5X - OsHLtsblnvT9ckEH1nlURKIhkGVnlQD5iHAEdyUaCUccbpqrELokMRQvHkKwIAgAnFNixqqqDKBv - X/OPOUy9L9vBLGvrLbfZfGwmznP7lmJqW02f9tDSp+T+l3J6lJRjnkSRPQfYX/2T5J8kslcI43Ae - yd40Cbnq/NkVSvu2nKnWGCKAgyJF/kv6BQAA//8DAKotCFkjAQAA - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:46:24 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - C7A4309F101B41010000000000000001.m_1 - NCBI-SID: - - C7A4309F101B4101_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=C7A4309F101B4101_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:46:24 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -4837,29 +4049,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603671&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603671\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603671/SRR1603671.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603671/SRR1603671_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603671/SRR1603671_2.fastq.gz\t819349138;6452549543;6449393711\tba486058bffc91225b827a35e209657c;b1ebed75e329fcf4f428cd91b383a0e7;c83664b7dc22a679de808221216e63ea\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603671\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603671/SRR1603671.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603671/SRR1603671_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/001/SRR1603671/SRR1603671_2.fastq.gz\t819349138;6452549543;6449393711\tba486058bffc91225b827a35e209657c;b1ebed75e329fcf4f428cd91b383a0e7;c83664b7dc22a679de808221216e63ea\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/001/SRR1603671\t11225823463\tcfe216bea8d7e708830e10004cbb441f\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:46:24 GMT + - Wed, 17 May 2023 18:38:31 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -4868,7 +4078,7 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' - request: body: null headers: @@ -4879,7 +4089,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRR1603672 response: @@ -4926,7 +4136,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:26 GMT + - Wed, 17 May 2023 18:38:31 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -4944,7 +4154,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRX725604 response: @@ -5003,7 +4213,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:26 GMT + - Wed, 17 May 2023 18:38:32 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5021,52 +4231,45 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRS716936 response: body: string: "\n\n\n \n - \ SRS716936\n SAMN03097510\n - \ GSM1519627\n \n + alias=\"GSM1519627\" center_name=\"Stanford University\" broker_name=\"NCBI\">\n + \ \n SRS716936\n SAMN03097510\n GSM1519627\n \n \ RNA-seq of the corpus callosum #5403\n \n \ 9606\n Homo sapiens\n - \ \n \n \n \n - \ ENA-STUDY\n SRP048683\n - \ \n \n \n - \ \n ENA-EXPERIMENT\n - \ SRX725604\n \n \n - \ \n \n ENA-RUN\n - \ SRR1603672\n \n \n - \ \n \n ENA-SUBMISSION\n - \ SRA188845\n \n \n - \ \n \n ARRAYEXPRESS\n - \ E-GEOD-62098\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n source_name\n - \ human brain\n \n - \ \n tissue\n frozen - postmortem brain from NICHD\n \n \n - \ disease\n autism spectrum disorder\n - \ \n \n ENA-SPOT-COUNT\n - \ 173441471\n \n \n - \ ENA-BASE-COUNT\n 30240407743\n - \ \n \n ENA-FIRST-PUBLIC\n - \ 2015-03-23\n \n - \ \n ENA-LAST-UPDATE\n - \ 2015-06-23\n \n + \ human\n \n \n + \ \n \n ENA-FASTQ-FILES\n + \ \n + \ \n \n \n \n + \ ENA-SUBMITTED-FILES\n \n + \ \n \n\n\n + \ \n INSDC secondary accession\n + \ SRS716936\n \n \n + \ NCBI submission package\n Generic.1.0\n + \ \n \n disease\n + \ autism spectrum disorder\n \n + \ \n organism\n Homo + sapiens\n \n \n + \ tissue\n frozen postmortem + brain from NICHD\n \n \n + \ source_name\n human brain\n + \ \n \n BioSampleModel\n + \ Generic\n \n \n + \ ENA-FIRST-PUBLIC\n 2015-01-01\n + \ \n \n ENA-LAST-UPDATE\n + \ 2015-01-01\n \n \ \n\n\n" headers: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:27 GMT + - Wed, 17 May 2023 18:38:33 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5084,7 +4287,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRP048683 response: @@ -5131,7 +4334,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:28 GMT + - Wed, 17 May 2023 18:38:33 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5149,7 +4352,7 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/browser/api/xml/SRA188845 response: @@ -5186,7 +4389,7 @@ interactions: Content-Type: - application/xml Date: - - Wed, 28 Jul 2021 19:46:28 GMT + - Wed, 17 May 2023 18:38:33 GMT Strict-Transport-Security: - max-age=0 Transfer-Encoding: @@ -5194,69 +4397,6 @@ interactions: status: code: 200 message: '' -- request: - body: acc=SRR1603672&accept-proto=fasp&version=2.0 - headers: - Accept: - - '*/*' - Accept-Encoding: - - gzip, deflate - Connection: - - keep-alive - Content-Length: - - '44' - User-Agent: - - python-requests/2.25.1 - method: POST - uri: https://www.ncbi.nlm.nih.gov/Traces/names/names.cgi - response: - body: - string: !!binary | - H4sIAAAAAAAAA5zOPWrEMBBA4T6nEKTYapeZ0Xgk+RKBzVbb6c84wUTGVhICc/h463SBV73qe6YL - PO1bXD+Tvl6vKGDFkSKJJXDgGZUA+YxwBrkRjQwj+7tONASPLgoy2RpqDER1KrYCQkhxUlUG0JeP - 5cec5t7X/WTWrfWW22LedhOXpX3XYqa2mT4fo6X3mvtfyuWrpBzzXBUdHR4ZxD5I7kEie4MwAh/d - FUj8AJLBScks3llOuTAULsUKDv8l/QIAAP//AwDto5TxIwEAAA== - headers: - Cache-Control: - - private - Connection: - - Keep-Alive - Content-Encoding: - - gzip - Content-Security-Policy: - - upgrade-insecure-requests - Content-Type: - - text/plain - Date: - - Wed, 28 Jul 2021 19:46:28 GMT - Keep-Alive: - - timeout=1, max=10 - NCBI-PHID: - - CC95032A101B41410000000000000001.m_1 - NCBI-SID: - - CC95032A101B4141_0000SID - Referrer-Policy: - - origin-when-cross-origin - Server: - - Apache - Set-Cookie: - - ncbi_sid=CC95032A101B4141_0000SID; domain=.nih.gov; path=/; expires=Thu, 28 - Jul 2022 19:46:28 GMT - SraNameServerOutputVersion: - - '2.0' - Strict-Transport-Security: - - max-age=31536000; includeSubDomains; preload - Transfer-Encoding: - - chunked - Vary: - - Accept-Encoding - X-UA-Compatible: - - IE=Edge - X-XSS-Protection: - - 1; mode=block - status: - code: 200 - message: OK - request: body: null headers: @@ -5267,29 +4407,27 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.25.1 + - python-requests/2.30.0 method: GET uri: https://www.ebi.ac.uk/ena/portal/api/filereport?accession=SRR1603672&result=read_run response: body: - string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603672\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603672/SRR1603672.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603672/SRR1603672_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603672/SRR1603672_2.fastq.gz\t829817369;7077631307;7106859277\t48400cd04f90d2d71139d6fa0efa1f6a;48c5e235e622c53dc0dc3e2f278cdc3d;7a09a8762df9b8982e956bf8e5b13663\t\t\t\t\t\t\n" + string: "run_accession\tfastq_ftp\tfastq_bytes\tfastq_md5\tbam_ftp\tbam_bytes\tbam_md5\tsubmitted_ftp\tsubmitted_bytes\tsubmitted_md5\tsra_ftp\tsra_bytes\tsra_md5\nSRR1603672\tftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603672/SRR1603672.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603672/SRR1603672_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR160/002/SRR1603672/SRR1603672_2.fastq.gz\t829817369;7077631307;7106859277\t48400cd04f90d2d71139d6fa0efa1f6a;48c5e235e622c53dc0dc3e2f278cdc3d;7a09a8762df9b8982e956bf8e5b13663\t\t\t\t\t\t\tftp.sra.ebi.ac.uk/vol1/srr/SRR160/002/SRR1603672\t12632070841\tf259817a61423e9ea922efd3e0109baf\n" headers: Cache-Control: - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '601' Content-Type: - text/plain Date: - - Wed, 28 Jul 2021 19:46:29 GMT + - Wed, 17 May 2023 18:38:33 GMT Expires: - '0' Pragma: - no-cache - Server: - - Apache-Coyote/1.1 Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked X-Content-Type-Options: - nosniff X-Frame-Options: @@ -5298,5 +4436,5 @@ interactions: - 1; mode=block status: code: 200 - message: OK + message: '' version: 1 From 646d1da6b8509a5046c1472249df6c08a6ddfc19 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 17 May 2023 14:51:44 -0400 Subject: [PATCH 129/220] remove NCBI ftp support --- foreman/data_refinery_foreman/surveyor/sra.py | 30 ++----------------- 1 file changed, 2 insertions(+), 28 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index 777a199ce..aaf685fc8 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -1,4 +1,3 @@ -import random import re import xml.etree.ElementTree as ET from typing import Dict, List @@ -19,7 +18,6 @@ SurveyJob, ) from data_refinery_common.rna_seq import _build_ena_file_url -from data_refinery_common.utils import get_fasp_sra_download from data_refinery_foreman.surveyor import harmony, utils from data_refinery_foreman.surveyor.external_source import ExternalSourceSurveyor @@ -33,14 +31,8 @@ "https://www.ebi.ac.uk/ena/portal/api/filereport?accession={accession}&result=read_run" ) NCBI_DOWNLOAD_URL_TEMPLATE = ( - "anonftp@ftp.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/" - "{first_three}/{first_six}/{accession}/{accession}.sra" + "https://sra-pub-run-odp.s3.amazonaws.com/sra/{accession}/{accession}" ) -NCBI_PRIVATE_DOWNLOAD_URL_TEMPLATE = ( - "anonftp@ftp-private.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/" - "{first_three}/{first_six}/{accession}/{accession}.sra" -) - class UnsupportedDataTypeError(Exception): pass @@ -338,25 +330,7 @@ def gather_all_metadata(run_accession): @staticmethod def _build_ncbi_file_url(run_accession: str): """Build the path to the hypothetical .sra file we want""" - accession = run_accession - first_three = accession[:3] - first_six = accession[:6] - - # Prefer the FASP-specific endpoints if possible.. - download_url = get_fasp_sra_download(run_accession) - - if not download_url: - # ..else, load balancing via coin flip. - if random.choice([True, False]): - download_url = NCBI_DOWNLOAD_URL_TEMPLATE.format( - first_three=first_three, first_six=first_six, accession=accession - ) - else: - download_url = NCBI_PRIVATE_DOWNLOAD_URL_TEMPLATE.format( - first_three=first_three, first_six=first_six, accession=accession - ) - - return download_url + return NCBI_DOWNLOAD_URL_TEMPLATE.format(accession=run_accession) @staticmethod def _apply_harmonized_metadata_to_sample(sample: Sample, metadata: dict): From dc133214499b3e3314c138a4ad80354a211aee8b Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 17 May 2023 14:52:13 -0400 Subject: [PATCH 130/220] update test to match new response values --- foreman/tests/surveyor/test_sra.py | 17 ++++------------- 1 file changed, 4 insertions(+), 13 deletions(-) diff --git a/foreman/tests/surveyor/test_sra.py b/foreman/tests/surveyor/test_sra.py index 0ec12f2b2..f0dbb3c9d 100644 --- a/foreman/tests/surveyor/test_sra.py +++ b/foreman/tests/surveyor/test_sra.py @@ -226,11 +226,9 @@ def test_metadata_is_gathered_correctly(self): self.assertEqual(metadata["run_ena_last_update"], "2017-08-11") self.assertEqual(metadata["run_ena_spot_count"], "32568360") self.assertEqual(metadata["sample_accession"], "DRS001521") - self.assertEqual(metadata["sample_center_name"], "BioSample") - self.assertEqual(metadata["sample_ena_base_count"], "3256836000") - self.assertEqual(metadata["sample_ena_first_public"], "2013-07-20") - self.assertEqual(metadata["sample_ena_last_update"], "2015-08-24") - self.assertEqual(metadata["sample_ena_spot_count"], "32568360") + self.assertEqual(metadata["sample_center_name"], "Group for Morphological Evolution, Center for Developmental Biology, Kobe Institute, RIKEN") + self.assertEqual(metadata["sample_ena_first_public"], "2013-02-27") + self.assertEqual(metadata["sample_ena_last_update"], "2014-11-12") self.assertEqual( metadata["sample_sample_comment"], ("mRNAseq of chicken at stage HH16 (biological " "replicate 1)"), @@ -255,14 +253,7 @@ def test_metadata_is_gathered_correctly(self): self.assertEqual(metadata["submission_title"], "Submitted by RIKEN_CDB on 19-JUL-2013") ncbi_url = SraSurveyor._build_ncbi_file_url(metadata["run_accession"]) - self.assertTrue( - ncbi_url - in [ - "anonftp@ftp.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/DRR/DRR002/DRR002116/DRR002116.sra", - "anonftp@ftp-private.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/DRR/DRR002/DRR002116/DRR002116.sra", - "dbtest@sra-download.ncbi.nlm.nih.gov:data/sracloud/traces/dra0/DRR/000002/DRR002116", - ] - ) + self.assertEqual(ncbi_url, "https://sra-pub-run-odp.s3.amazonaws.com/sra/DRR002116/DRR002116") def test_sra_metadata_is_harmonized(self): metadata = SraSurveyor.gather_all_metadata("SRR3098582") From 49a60f2b7cad785a43d519fc9ffd05c382849bc2 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 17 May 2023 14:52:41 -0400 Subject: [PATCH 131/220] remove unused imports --- workers/data_refinery_workers/downloaders/sra.py | 2 +- workers/tests/downloaders/test_sra.py | 1 - 2 files changed, 1 insertion(+), 2 deletions(-) diff --git a/workers/data_refinery_workers/downloaders/sra.py b/workers/data_refinery_workers/downloaders/sra.py index c0268dc5a..9008f0d4d 100644 --- a/workers/data_refinery_workers/downloaders/sra.py +++ b/workers/data_refinery_workers/downloaders/sra.py @@ -17,7 +17,7 @@ Sample, ) from data_refinery_common.rna_seq import _build_ena_file_url -from data_refinery_common.utils import download_file, get_env_variable, get_https_sra_download +from data_refinery_common.utils import download_file, get_env_variable from data_refinery_workers.downloaders import utils logger = get_and_configure_logger(__name__) diff --git a/workers/tests/downloaders/test_sra.py b/workers/tests/downloaders/test_sra.py index 8c969043c..674c2e04d 100644 --- a/workers/tests/downloaders/test_sra.py +++ b/workers/tests/downloaders/test_sra.py @@ -1,5 +1,4 @@ import os -from unittest.mock import patch from django.test import TestCase, tag From ac0fb581753c38ac388cae6a629d0b39889fef88 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 17 May 2023 15:17:00 -0400 Subject: [PATCH 132/220] formatting --- foreman/data_refinery_foreman/surveyor/sra.py | 10 ++++------ foreman/tests/surveyor/test_sra.py | 9 +++++++-- 2 files changed, 11 insertions(+), 8 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index aaf685fc8..d414dbffa 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -30,9 +30,8 @@ ENA_FILE_REPORT_URL_TEMPLATE = ( "https://www.ebi.ac.uk/ena/portal/api/filereport?accession={accession}&result=read_run" ) -NCBI_DOWNLOAD_URL_TEMPLATE = ( - "https://sra-pub-run-odp.s3.amazonaws.com/sra/{accession}/{accession}" -) +NCBI_DOWNLOAD_URL_TEMPLATE = "https://sra-pub-run-odp.s3.amazonaws.com/sra/{accession}/{accession}" + class UnsupportedDataTypeError(Exception): pass @@ -58,7 +57,6 @@ def source_type(self): @staticmethod def gather_submission_metadata(metadata: Dict) -> None: - formatted_metadata_URL = ENA_METADATA_URL_TEMPLATE.format(metadata["submission_accession"]) response = utils.requests_retry_session().get(formatted_metadata_URL) submission_xml = ET.fromstring(response.text)[0] @@ -182,7 +180,8 @@ def gather_file_report(run_accession: str) -> List[Dict]: This endpoint returns a weird format, so some custom parsing is required: run_accession fastq_ftp fastq_bytes fastq_md5 submitted_ftp submitted_bytes submitted_md5 sra_ftp sra_bytes sra_md5 - SRR7353755 ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_2.fastq.gz 25176;2856704;3140575 7ef1ba010dcb679217112efa380798b2;6bc5651b7103306d4d65018180ab8d0d;3856c14164612d9879d576a046a9879f""" + SRR7353755 ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_2.fastq.gz 25176;2856704;3140575 7ef1ba010dcb679217112efa380798b2;6bc5651b7103306d4d65018180ab8d0d;3856c14164612d9879d576a046a9879f + """ response = utils.requests_retry_session().get( ENA_FILE_REPORT_URL_TEMPLATE.format(accession=run_accession) ) @@ -603,7 +602,6 @@ def discover_experiment_and_samples(self): accessions_to_run = [] for child in study_links: if child[0][0].text == "ENA-RUN": - all_runs = child[0][1].text # Ranges can be disjoint, separated by commas diff --git a/foreman/tests/surveyor/test_sra.py b/foreman/tests/surveyor/test_sra.py index f0dbb3c9d..35e922d21 100644 --- a/foreman/tests/surveyor/test_sra.py +++ b/foreman/tests/surveyor/test_sra.py @@ -226,7 +226,10 @@ def test_metadata_is_gathered_correctly(self): self.assertEqual(metadata["run_ena_last_update"], "2017-08-11") self.assertEqual(metadata["run_ena_spot_count"], "32568360") self.assertEqual(metadata["sample_accession"], "DRS001521") - self.assertEqual(metadata["sample_center_name"], "Group for Morphological Evolution, Center for Developmental Biology, Kobe Institute, RIKEN") + self.assertEqual( + metadata["sample_center_name"], + "Group for Morphological Evolution, Center for Developmental Biology, Kobe Institute, RIKEN" + ) self.assertEqual(metadata["sample_ena_first_public"], "2013-02-27") self.assertEqual(metadata["sample_ena_last_update"], "2014-11-12") self.assertEqual( @@ -253,7 +256,9 @@ def test_metadata_is_gathered_correctly(self): self.assertEqual(metadata["submission_title"], "Submitted by RIKEN_CDB on 19-JUL-2013") ncbi_url = SraSurveyor._build_ncbi_file_url(metadata["run_accession"]) - self.assertEqual(ncbi_url, "https://sra-pub-run-odp.s3.amazonaws.com/sra/DRR002116/DRR002116") + self.assertEqual( + ncbi_url, "https://sra-pub-run-odp.s3.amazonaws.com/sra/DRR002116/DRR002116" + ) def test_sra_metadata_is_harmonized(self): metadata = SraSurveyor.gather_all_metadata("SRR3098582") From f6c5389cae172cf71fa7587032094fca7c32723a Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 17 May 2023 15:21:09 -0400 Subject: [PATCH 133/220] add trailing comma --- foreman/tests/surveyor/test_sra.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/tests/surveyor/test_sra.py b/foreman/tests/surveyor/test_sra.py index 35e922d21..1a098409a 100644 --- a/foreman/tests/surveyor/test_sra.py +++ b/foreman/tests/surveyor/test_sra.py @@ -228,7 +228,7 @@ def test_metadata_is_gathered_correctly(self): self.assertEqual(metadata["sample_accession"], "DRS001521") self.assertEqual( metadata["sample_center_name"], - "Group for Morphological Evolution, Center for Developmental Biology, Kobe Institute, RIKEN" + "Group for Morphological Evolution, Center for Developmental Biology, Kobe Institute, RIKEN", ) self.assertEqual(metadata["sample_ena_first_public"], "2013-02-27") self.assertEqual(metadata["sample_ena_last_update"], "2014-11-12") From e867dc1056ba41f2930e911a149ba7d2897ca5ce Mon Sep 17 00:00:00 2001 From: David Mejia Date: Thu, 18 May 2023 13:51:57 -0400 Subject: [PATCH 134/220] remove unused imports --- workers/tests/processors/test_no_op.py | 2 -- 1 file changed, 2 deletions(-) diff --git a/workers/tests/processors/test_no_op.py b/workers/tests/processors/test_no_op.py index 4d2cf47e6..861e3e0e2 100644 --- a/workers/tests/processors/test_no_op.py +++ b/workers/tests/processors/test_no_op.py @@ -1,4 +1,3 @@ -import math import os import shutil from pathlib import Path @@ -6,7 +5,6 @@ from django.test import TestCase, tag import pandas as pd -import scipy.stats from data_refinery_common.models import ( Organism, From 93a5c05a04cc677392392f25c1c4ad64e28f4040 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Thu, 18 May 2023 13:53:07 -0400 Subject: [PATCH 135/220] update tests utils import --- workers/tests/processors/test_no_op.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workers/tests/processors/test_no_op.py b/workers/tests/processors/test_no_op.py index 861e3e0e2..83eed5953 100644 --- a/workers/tests/processors/test_no_op.py +++ b/workers/tests/processors/test_no_op.py @@ -5,6 +5,7 @@ from django.test import TestCase, tag import pandas as pd +from tests.utils import assertMostlyAgrees from data_refinery_common.models import ( Organism, @@ -15,7 +16,6 @@ Sample, ) from data_refinery_workers.processors import no_op -from data_refinery_workers.processors.testing_utils import assertMostlyAgrees def prepare_job(job_info: dict) -> ProcessorJob: From 25eb2b13bbeda5bae6020e10966a8469a3a9430f Mon Sep 17 00:00:00 2001 From: David Mejia Date: Thu, 18 May 2023 17:25:06 -0400 Subject: [PATCH 136/220] add free-disk-space --- .github/workflows/config.yml | 25 +++++++++++++++++++++---- 1 file changed, 21 insertions(+), 4 deletions(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 94c906279..06a02840d 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -40,7 +40,11 @@ jobs: - uses: actions/checkout@v3 - name: Free up space - run: ./.github/scripts/cleanup_instance.sh + uses: jlumbroso/free-disk-space@main + with: + # this might remove tools that are actually needed, + # if set to "true" but frees about 6 GB + tool-cache: false - name: Login to Packages Container registry uses: docker/login-action@v2 @@ -128,7 +132,11 @@ jobs: - uses: actions/checkout@v3 - name: Free up space - run: ./.github/scripts/cleanup_instance.sh + uses: jlumbroso/free-disk-space@main + with: + # this might remove tools that are actually needed, + # if set to "true" but frees about 6 GB + tool-cache: false - name: Login to Packages Container registry uses: docker/login-action@v2 @@ -229,7 +237,11 @@ jobs: - uses: actions/checkout@v3 - name: Free up space - run: ./.github/scripts/cleanup_instance.sh + uses: jlumbroso/free-disk-space@main + with: + # this might remove tools that are actually needed, + # if set to "true" but frees about 6 GB + tool-cache: false - name: Login to Packages Container registry uses: docker/login-action@v2 @@ -364,8 +376,13 @@ jobs: CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} steps: - uses: actions/checkout@v3 + - name: Free up space - run: ./.github/scripts/cleanup_instance.sh + uses: jlumbroso/free-disk-space@main + with: + # this might remove tools that are actually needed, + # if set to "true" but frees about 6 GB + tool-cache: false - name: Login to Packages Container registry uses: docker/login-action@v2 From f597413bb87de2a8c359f9948fc92662fed3beef Mon Sep 17 00:00:00 2001 From: David Mejia Date: Thu, 18 May 2023 17:46:07 -0400 Subject: [PATCH 137/220] free space on downloader_no_op_tests --- .github/workflows/config.yml | 7 +++++++ 1 file changed, 7 insertions(+) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 06a02840d..1c2dc860f 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -89,6 +89,13 @@ jobs: steps: - uses: actions/checkout@v3 + - name: Free up space + uses: jlumbroso/free-disk-space@main + with: + # this might remove tools that are actually needed, + # if set to "true" but frees about 6 GB + tool-cache: false + - name: Login to Packages Container registry uses: docker/login-action@v2 with: From 778cfd57f4676ff903797337a016fa72e75d92a7 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 19 May 2023 16:02:03 -0400 Subject: [PATCH 138/220] codecov informational --- codecov.yml | 13 +++++++++++++ 1 file changed, 13 insertions(+) diff --git a/codecov.yml b/codecov.yml index 77707aa1d..59eaf09fe 100644 --- a/codecov.yml +++ b/codecov.yml @@ -1,2 +1,15 @@ github_checks: annotations: false + +ignore: + - "**/tests/**" + - "**/*.sh" + +coverage: + status: + project: + default: + informational: true + patch: + default: + informational: true From 6071787a2e4c0c03973a6af53c5a64bc229675d0 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 13 Jun 2023 09:17:43 -0700 Subject: [PATCH 139/220] Implement Docker image cache cross-environment support - Name Docker images based on the branch hash value (shasum) - Add setup.py PEP-440 workaround for common package - Reorganize GHA jobs - Utilize Docker registry cache for image cache layers sharing - Use separate Docker images (_cache) for caching - Optimize Docker images size (foreman, salmon) - Add remote builder support (used by default in GHA jobs) - Set umask explicitly to avoid cache miss due to permissions diff - Introduce API base image, derive api local/production from it - Reformat affected files for better readability - Use better names for scripts/variables - Consolidate renv related scripts location (use common/ everywhere) - Retire -d option of `prepare_image.sh` - Delete affymetrix_local image as no longer used (affymetrix cache boost satisfies our tests execution time requirements) - Sort .dockerignore entries - Update .pre-commit-config.yaml - Update README --- .dockerignore | 21 +- .github/scripts/cleanup_instance.sh | 9 +- .github/scripts/filter_tests.sh | 6 +- .github/scripts/post_deploy_cleanup.sh | 3 +- .github/scripts/pull_docker_images.sh | 5 +- .github/scripts/push_docker_images.sh | 5 +- .github/scripts/remote_deploy.sh | 21 +- .github/scripts/run_terraform.sh | 12 +- .github/scripts/slackpost_deploy.sh | 19 +- .github/scripts/slackpost_end_to_end.sh | 19 +- ...e_docker_img.sh => update_docker_image.sh} | 53 +- .github/scripts/upload_test_coverage.sh | 11 +- .github/workflows/config.yml | 678 ++++++++++-------- .pre-commit-config.yaml | 19 +- README.md | 99 +-- api/dockerfiles/Dockerfile.api_base | 29 + api/dockerfiles/Dockerfile.api_local | 31 +- api/dockerfiles/Dockerfile.api_production | 31 +- api/run_tests.sh | 39 +- api/serve.sh | 23 +- api/serve_production.sh | 27 +- common/R/renv_load.R | 15 +- {workers => common}/R/renv_save.R | 0 common/dockerfiles/Dockerfile.common_tests | 5 +- common/dockerfiles/Dockerfile.migrations | 5 +- common/run_tests.sh | 38 +- common/setup.py | 16 +- foreman/dockerfiles/Dockerfile.foreman | 16 +- foreman/run_end_to_end_tests.sh | 28 +- foreman/run_management_command.sh | 27 +- foreman/run_tests.sh | 32 +- foreman/test_survey.sh | 29 +- .../api-server-instance-user-data.tpl.sh | 54 +- infrastructure/deploy.sh | 170 ++--- infrastructure/deploy_box_instance_data.sh | 1 + infrastructure/destroy_terraform.sh | 17 +- .../foreman-server-instance-user-data.tpl.sh | 113 +-- scripts/common.sh | 112 ++- scripts/format_batch_with_env.sh | 138 ++-- scripts/install_all.sh | 165 ++--- scripts/install_db_docker.sh | 49 +- scripts/kill_all_jobs.sh | 7 +- scripts/make_migrations.sh | 40 +- scripts/prepare_image.sh | 189 ++--- scripts/rebuild_es_index.sh | 5 +- scripts/reinit_database.sh | 6 +- scripts/run_all_tests.sh | 5 +- scripts/run_es.sh | 18 +- scripts/run_manage.sh | 54 +- scripts/run_postgres.sh | 31 +- scripts/run_psql_shell.sh | 10 +- scripts/run_shell.sh | 29 +- scripts/update_docker_images.sh | 136 ++++ scripts/update_models.sh | 21 +- scripts/update_my_docker_images.sh | 126 ---- workers/R/renv_load.R | 16 - workers/dockerfiles/Dockerfile.affymetrix | 7 +- .../dockerfiles/Dockerfile.affymetrix_local | 29 - workers/dockerfiles/Dockerfile.compendia | 5 +- workers/dockerfiles/Dockerfile.downloaders | 7 +- workers/dockerfiles/Dockerfile.illumina | 7 +- workers/dockerfiles/Dockerfile.no_op | 7 +- workers/dockerfiles/Dockerfile.salmon | 12 +- workers/dockerfiles/Dockerfile.smasher | 7 +- workers/dockerfiles/Dockerfile.transcriptome | 20 +- workers/run_command.sh | 44 +- workers/run_janitor.sh | 37 +- workers/run_job.sh | 141 ++-- workers/run_tests.sh | 208 +++--- 69 files changed, 1869 insertions(+), 1545 deletions(-) rename .github/scripts/{update_docker_img.sh => update_docker_image.sh} (59%) create mode 100644 api/dockerfiles/Dockerfile.api_base rename {workers => common}/R/renv_save.R (100%) create mode 100755 scripts/update_docker_images.sh delete mode 100755 scripts/update_my_docker_images.sh delete mode 100644 workers/R/renv_load.R delete mode 100644 workers/dockerfiles/Dockerfile.affymetrix_local diff --git a/.dockerignore b/.dockerignore index cfd8a7fed..c2205b57e 100644 --- a/.dockerignore +++ b/.dockerignore @@ -1,16 +1,15 @@ -volume -*/volume -test_volume -*/test_volume -volume .git +*/batch-job-specs +*/test_volume +*/volume +config/externally_supplied_metadata/metasra/*.tab +config/externally_supplied_metadata/metasra/metasra_keywords.json +config/externally_supplied_metadata/metasra/metasra_translated.json +config/externally_supplied_metadata/metasra/SRAmetadb.sqlite dr_env -volumes_postgres/ env/ infrastructure/ terraform/ -*/batch-job-specs -config/externally_supplied_metadata/metasra/metasra_translated.json -config/externally_supplied_metadata/metasra/metasra_keywords.json -config/externally_supplied_metadata/metasra/*.tab -config/externally_supplied_metadata/metasra/SRAmetadb.sqlite +test_volume +volume +volumes_postgres/ diff --git a/.github/scripts/cleanup_instance.sh b/.github/scripts/cleanup_instance.sh index d270e1582..d359f6966 100755 --- a/.github/scripts/cleanup_instance.sh +++ b/.github/scripts/cleanup_instance.sh @@ -13,7 +13,14 @@ sudo apt-get remove -y '^ghc-8.*' sudo apt-get remove -y '^dotnet-.*' sudo apt-get remove -y '^llvm-.*' sudo apt-get remove -y 'php.*' -sudo apt-get remove -y azure-cli google-cloud-sdk hhvm google-chrome-stable firefox powershell mono-devel +sudo apt-get remove -y \ + azure-cli \ + firefox \ + google-chrome-stable \ + google-cloud-sdk \ + hhvm \ + mono-devel \ + powershell sudo apt-get autoremove -y sudo apt-get clean diff --git a/.github/scripts/filter_tests.sh b/.github/scripts/filter_tests.sh index fca0caec3..88a3dfff5 100755 --- a/.github/scripts/filter_tests.sh +++ b/.github/scripts/filter_tests.sh @@ -1,14 +1,14 @@ #!/bin/bash -# Exit on failure +# Exit on failure. set -e git log --format=oneline -n 1 "$GITHUB_SHA" if [[ $(git log --format=oneline -n 1 "$GITHUB_SHA") = *"noslow"* ]]; then - echo "Skipping slow tests.."; + echo "Skipping slow tests..." ./workers/run_tests.sh --exclude-tag=slow "$@" else - echo "Running all tests.."; + echo "Running all tests..." ./workers/run_tests.sh "$@" fi diff --git a/.github/scripts/post_deploy_cleanup.sh b/.github/scripts/post_deploy_cleanup.sh index 467cbc50e..9901a3b26 100755 --- a/.github/scripts/post_deploy_cleanup.sh +++ b/.github/scripts/post_deploy_cleanup.sh @@ -8,4 +8,5 @@ ssh -o StrictHostKeyChecking=no \ -o ServerAliveInterval=15 \ -i infrastructure/data-refinery-key.pem \ - ubuntu@"${DEPLOY_IP_ADDRESS}" "cd refinebio && git clean -f" + "ubuntu@${DEPLOY_IP_ADDRESS}" \ + "cd refinebio && git clean -f" diff --git a/.github/scripts/pull_docker_images.sh b/.github/scripts/pull_docker_images.sh index c691a0b41..532d0ccab 100755 --- a/.github/scripts/pull_docker_images.sh +++ b/.github/scripts/pull_docker_images.sh @@ -2,14 +2,15 @@ set -e -REPO=$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]') if [ -z "$IMAGES" ]; then echo "Error: must put images to pull in \$IMAGES" >&2 exit 1 fi +REPO=$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]') + for image in $IMAGES; do PACKAGE="$REPO/dr_$image" - # Only pull the package if it already exists + # Only pull the package if it already exists. (docker pull "$PACKAGE" && docker tag "$PACKAGE" "ccdlstaging/dr_$image") || true done diff --git a/.github/scripts/push_docker_images.sh b/.github/scripts/push_docker_images.sh index 738290c95..ccd79d424 100755 --- a/.github/scripts/push_docker_images.sh +++ b/.github/scripts/push_docker_images.sh @@ -1,11 +1,14 @@ #!/bin/sh -REPO=$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]') +set -e + if [ -z "$IMAGES" ]; then echo "Error: must put images to pull in \$IMAGES" >&2 exit 1 fi +REPO=$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]') + for image in $IMAGES; do PACKAGE="$REPO/dr_$image" docker tag "ccdlstaging/dr_$image" "$PACKAGE" diff --git a/.github/scripts/remote_deploy.sh b/.github/scripts/remote_deploy.sh index 318aaac0c..aabfd0b3b 100755 --- a/.github/scripts/remote_deploy.sh +++ b/.github/scripts/remote_deploy.sh @@ -18,11 +18,10 @@ # - AWS_ACCESS_KEY_ID -- The AWS key id to use when interacting with AWS. # - AWS_SECRET_ACCESS_KEY -- The AWS secret key to use when interacting with AWS. - -echo "$INSTANCE_SSH_KEY" > infrastructure/data-refinery-key.pem +echo "$INSTANCE_SSH_KEY" >infrastructure/data-refinery-key.pem chmod 600 infrastructure/data-refinery-key.pem -run_on_deploy_box () { +run_on_deploy_box() { # shellcheck disable=SC2029 ssh -o StrictHostKeyChecking=no \ -o ServerAliveInterval=15 \ @@ -32,7 +31,7 @@ run_on_deploy_box () { # Create file containing local env vars that are needed for deploy. rm -f env_vars -cat >> env_vars <>env_vars <&1 | tee -a /var/log/docker_update_$CI_TAG.log" -run_on_deploy_box "source env_vars && ./.github/scripts/update_docker_img.sh 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" +run_on_deploy_box "source env_vars && ./.github/scripts/update_docker_image.sh 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" run_on_deploy_box "source env_vars && echo -e '######\nFinished building new images for $CI_TAG\n######' 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" -# Load docker_img_exists function and $ALL_CCDL_IMAGES -source scripts/common.sh +# Load docker_image_exists function and $ALL_IMAGES. +. ./scripts/common.sh if [[ "$MASTER_OR_DEV" == "master" ]]; then DOCKERHUB_REPO=ccdl elif [[ "$MASTER_OR_DEV" == "dev" ]]; then DOCKERHUB_REPO=ccdlstaging else - echo "Why in the world was remote_deploy.sh called from a branch other than dev or master?!?!?" + echo "Why in the world was remote_deploy.sh called from a branch other than dev or master?!" exit 1 fi @@ -89,10 +88,10 @@ fi # https://github.com/AlexsLemonade/refinebio/issues/784 # Since it's not clear how that happened, the safest thing is to add # an explicit check that the Docker images were successfully updated. -for IMAGE in $ALL_CCDL_IMAGES; do +for IMAGE in $ALL_IMAGES; do image_name="$DOCKERHUB_REPO/dr_$IMAGE" - if ! docker_img_exists "$image_name" "$CI_TAG"; then - echo "Docker image $image_name:$CI_TAG doesn't exist after running update_docker_img.sh!" + if ! docker_image_exists "$image_name" "$CI_TAG"; then + echo "Docker image $image_name:$CI_TAG doesn't exist after running update_docker_image.sh!" echo "This is generally caused by a temporary error, please try the 'Rerun workflow' button." exit 1 fi diff --git a/.github/scripts/run_terraform.sh b/.github/scripts/run_terraform.sh index 72402e520..547b8c208 100755 --- a/.github/scripts/run_terraform.sh +++ b/.github/scripts/run_terraform.sh @@ -1,17 +1,15 @@ #!/bin/bash -e -# Import Hashicorps' Key. +# Import Hashicorps' key. curl https://keybase.io/hashicorp/pgp_keys.asc | gpg --import - -# Install terraform and nomad +# Install Terraform. cd TERRAFORM_VERSION=0.13.5 wget -N https://releases.hashicorp.com/terraform/$TERRAFORM_VERSION/terraform_${TERRAFORM_VERSION}_linux_amd64.zip wget -N https://releases.hashicorp.com/terraform/$TERRAFORM_VERSION/terraform_${TERRAFORM_VERSION}_SHA256SUMS wget -N https://releases.hashicorp.com/terraform/$TERRAFORM_VERSION/terraform_${TERRAFORM_VERSION}_SHA256SUMS.sig - # Verify the signature file is untampered. gpg_ok=$(gpg --verify terraform_${TERRAFORM_VERSION}_SHA256SUMS.sig terraform_${TERRAFORM_VERSION}_SHA256SUMS |& grep Good) if [[ "$gpg_ok" == "" ]]; then @@ -32,9 +30,9 @@ sudo mv terraform /usr/local/bin/ cd ~/refinebio/infrastructure # Circle won't set the branch name for us, so do it ourselves. -source ~/refinebio/scripts/common.sh -branch=$(get_master_or_dev "$CI_TAG") +. ~/refinebio/scripts/common.sh +branch=$(get_master_or_dev "$CI_TAG") if [[ $branch == "master" ]]; then ENVIRONMENT=prod BATCH_USE_ON_DEMAND_INSTANCES="false" @@ -42,7 +40,7 @@ elif [[ $branch == "dev" ]]; then ENVIRONMENT=staging BATCH_USE_ON_DEMAND_INSTANCES="true" else - echo "Why in the world was run_terraform.sh called from a branch other than dev or master?!?!?" + echo "Why in the world was run_terraform.sh called from a branch other than dev or master?!" exit 1 fi diff --git a/.github/scripts/slackpost_deploy.sh b/.github/scripts/slackpost_deploy.sh index 9d9b501fb..707de3e17 100755 --- a/.github/scripts/slackpost_deploy.sh +++ b/.github/scripts/slackpost_deploy.sh @@ -1,26 +1,23 @@ #!/bin/bash -if [[ $ENGAGEMENTBOT_WEBHOOK == "" ]] -then +if [[ $ENGAGEMENTBOT_WEBHOOK == "" ]]; then echo "No webhook url. Set ENGAGEMENTBOT_WEBHOOK in the environment variables if you want to be notified of deploys on slack" exit 0 fi # ------------ channel=$1 -if [[ $channel == "" ]] -then - echo "No channel specified" - exit 1 +if [[ $channel == "" ]]; then + echo "No channel specified" + exit 1 fi # ------------ shift username=$1 -if [[ $username == "" ]] -then - echo "No username specified" - exit 1 +if [[ $username == "" ]]; then + echo "No username specified" + exit 1 fi # ------------ @@ -35,7 +32,7 @@ fi text="New deployment! Woo! $CI_USERNAME: $CI_BRANCH $CI_TAG" -escapedText=$(echo "$text" | sed 's/"/\"/g' | sed "s/'/\'/g" ) +escapedText=$(echo "$text" | sed 's/"/\"/g' | sed "s/'/\'/g") json="{\"channel\": \"$channel\", \"username\":\"$username\", \"icon_emoji\":\":tada:\", \"attachments\":[{\"color\":\"danger\" , \"text\": \"$escapedText\"}]}" diff --git a/.github/scripts/slackpost_end_to_end.sh b/.github/scripts/slackpost_end_to_end.sh index 7fa2e71fc..821213b6e 100755 --- a/.github/scripts/slackpost_end_to_end.sh +++ b/.github/scripts/slackpost_end_to_end.sh @@ -1,31 +1,28 @@ #!/bin/bash -if [[ $ENGAGEMENTBOT_WEBHOOK == "" ]] -then +if [[ $ENGAGEMENTBOT_WEBHOOK == "" ]]; then echo "No webhook url. Set ENGAGEMENTBOT_WEBHOOK in the environment variables if you want to be notified of deploys on slack" exit 0 fi # ------------ channel=$1 -if [[ $channel == "" ]] -then - echo "No channel specified" - exit 1 +if [[ $channel == "" ]]; then + echo "No channel specified" + exit 1 fi # ------------ shift username=$1 -if [[ $username == "" ]] -then - echo "No username specified" - exit 1 +if [[ $username == "" ]]; then + echo "No username specified" + exit 1 fi text="The end-to-end tests passed in the staging stack!!!" -escapedText=$(echo "$text" | sed 's/"/\"/g' | sed "s/'/\'/g" ) +escapedText=$(echo "$text" | sed 's/"/\"/g' | sed "s/'/\'/g") json="{\"channel\": \"$channel\", \"username\":\"$username\", \"icon_emoji\":\":tada:\", \"attachments\":[{\"color\":\"danger\" , \"text\": \"$escapedText\"}]}" diff --git a/.github/scripts/update_docker_img.sh b/.github/scripts/update_docker_image.sh similarity index 59% rename from .github/scripts/update_docker_img.sh rename to .github/scripts/update_docker_image.sh index 620149463..a7aa97747 100755 --- a/.github/scripts/update_docker_img.sh +++ b/.github/scripts/update_docker_image.sh @@ -1,8 +1,8 @@ #!/bin/bash set -e -# Load docker_img_exists function and $CCDL_WORKER_IMAGES -source ~/refinebio/scripts/common.sh +# Load docker_image_exists function and $WORKER_IMAGES. +. ~/refinebio/scripts/common.sh # Github won't set the branch name for us, so do it ourselves. branch=$(get_master_or_dev "$CI_TAG") @@ -12,71 +12,74 @@ if [[ "$branch" == "master" ]]; then elif [[ "$branch" == "dev" ]]; then DOCKERHUB_REPO=ccdlstaging else - echo "Why in the world was update_docker_img.sh called from a branch other than dev or master?!?!?" + echo "Why in the world was update_docker_image.sh called from a branch other than dev or master!?" exit 1 fi -echo "$CI_TAG" > ~/refinebio/common/version +echo "$CI_TAG" >~/refinebio/common/version # Create ~/refinebio/common/dist/data-refinery-common-*.tar.gz, which is # required by the workers and data_refinery_foreman images. -## Remove old common distributions if they exist +## Remove old common distributions if they exist. rm -f ~/refinebio/common/dist/* cd ~/refinebio/common && python3 setup.py sdist -# Log into DockerHub +# Log into DockerHub. docker login -u "$DOCKER_ID" -p "$DOCKER_PASSWD" cd ~/refinebio -for IMAGE in $CCDL_WORKER_IMAGES; do +for IMAGE in $WORKER_IMAGES; do image_name="$DOCKERHUB_REPO/dr_$IMAGE" - if docker_img_exists "$image_name" "$CI_TAG"; then + if docker_image_exists "$image_name" "$CI_TAG"; then echo "Docker image exists, skipping: $image_name:$CI_TAG" else echo "Building docker image: $image_name:$CI_TAG" # Build and push image. We use the CI_TAG as the system version. docker build \ - -t "$image_name:$CI_TAG" \ - -f "workers/dockerfiles/Dockerfile.$IMAGE" \ - --build-arg SYSTEM_VERSION="$CI_TAG" . + --build-arg SYSTEM_VERSION="$CI_TAG" \ + --file "workers/dockerfiles/Dockerfile.$IMAGE" \ + --tag "$image_name:$CI_TAG" \ + . docker push "$image_name:$CI_TAG" - # Update latest version + # Update latest version. docker tag "$image_name:$CI_TAG" "$image_name:latest" docker push "$image_name:latest" - # Save some space when we're through + # Save some space when we're through. docker rmi "$image_name:$CI_TAG" fi done -# Build and push foreman image +# Build and push foreman image. FOREMAN_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_foreman" -if docker_img_exists "$FOREMAN_DOCKER_IMAGE" "$CI_TAG"; then +if docker_image_exists "$FOREMAN_DOCKER_IMAGE" "$CI_TAG"; then echo "Docker image exists, skipping: $FOREMAN_DOCKER_IMAGE:$CI_TAG" else # Build and push image. We use the CI_TAG as the system version. docker build \ - -t "$FOREMAN_DOCKER_IMAGE:$CI_TAG" \ - -f foreman/dockerfiles/Dockerfile.foreman \ - --build-arg SYSTEM_VERSION="$CI_TAG" . + --build-arg SYSTEM_VERSION="$CI_TAG" \ + --file foreman/dockerfiles/Dockerfile.foreman \ + --tag "$FOREMAN_DOCKER_IMAGE:$CI_TAG" \ + . docker push "$FOREMAN_DOCKER_IMAGE:$CI_TAG" - # Update latest version + # Update latest version. docker tag "$FOREMAN_DOCKER_IMAGE:$CI_TAG" "$FOREMAN_DOCKER_IMAGE:latest" docker push "$FOREMAN_DOCKER_IMAGE:latest" fi -# Build and push API image +# Build and push API image. API_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_api" -if docker_img_exists "$API_DOCKER_IMAGE" "$CI_TAG"; then +if docker_image_exists "$API_DOCKER_IMAGE" "$CI_TAG"; then echo "Docker image exists, skipping: $API_DOCKER_IMAGE:$CI_TAG" else # Build and push image. We use the CI_TAG as the system version. docker build \ - -t "$API_DOCKER_IMAGE:$CI_TAG" \ - -f api/dockerfiles/Dockerfile.api_production \ - --build-arg SYSTEM_VERSION="$CI_TAG" . + --build-arg SYSTEM_VERSION="$CI_TAG" \ + --file api/dockerfiles/Dockerfile.api_production \ + --tag "$API_DOCKER_IMAGE:$CI_TAG" \ + . docker push "$API_DOCKER_IMAGE:$CI_TAG" - # Update latest version + # Update latest version. docker tag "$API_DOCKER_IMAGE:$CI_TAG" "$API_DOCKER_IMAGE:latest" docker push "$API_DOCKER_IMAGE:latest" fi diff --git a/.github/scripts/upload_test_coverage.sh b/.github/scripts/upload_test_coverage.sh index 44a009cee..12068f485 100755 --- a/.github/scripts/upload_test_coverage.sh +++ b/.github/scripts/upload_test_coverage.sh @@ -3,14 +3,12 @@ # Script to upload code coverage project=$1 -if [[ $project == "" ]] -then +if [[ $project == "" ]]; then echo "No project specified" exit 1 fi -if [[ $project == "workers" ]] -then +if [[ $project == "workers" ]]; then # the workers project uses it's own test_volume directory test_volume="workers/test_volume" else @@ -19,8 +17,7 @@ fi coverage_file="${test_volume}/coverage.xml" -if [[ ! -f $coverage_file ]] -then +if [[ ! -f $coverage_file ]]; then echo "Coverage file wasn't found, were the tests run before?" exit 0 # exit this script but don't fail the tests for this. fi @@ -30,7 +27,7 @@ output_file="${test_volume}/${project}_coverage.xml" # In the test coverage report, all file paths are relative to each project # folder. We need to be relative to the repo's root directory. That's why we # append the project folder name to each file path in coverage.xml -sed "s/filename=\"/filename=\"$project\//g" "$coverage_file" > "$output_file" +sed "s/filename=\"/filename=\"$project\//g" "$coverage_file" >"$output_file" # codecov.sh is located at https://codecov.io/bash # we downloaded it for convenience diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 1c2dc860f..9bbddd958 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -1,513 +1,552 @@ -name: test-and-deploy +name: refine.bio CI/CD + +env: + CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + DEPLOY_IP_ADDRESS: ${{ secrets.DEPLOY_IP_ADDRESS }} + DOCKER_BUILDKIT: 1 + DOCKER_ID: ${{ secrets.DOCKER_ID }} + DOCKER_PASSWD: ${{ secrets.DOCKER_PASSWD }} + DOCKER_IO_USERNAME: ${{ secrets.DOCKER_IO_USERNAME }} + DOCKER_IO_PASSWORD: ${{ secrets.DOCKER_IO_PASSWORD }} + INSTANCE_SSH_KEY: ${{ secrets.INSTANCE_SSH_KEY }} on: push jobs: - syntax_test: - # ubuntu-latest is ubuntu 18.04 which has a really outdated version of shellcheck - runs-on: ubuntu-20.04 + # Code quality check jobs. + check_syntax: + name: Check Syntax env: AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - + - uses: actions/setup-python@v3 - uses: hashicorp/setup-terraform@v2 with: terraform_version: 0.13.5 - - name: Install build & test dependencies - run: | - python -m pip install --upgrade pip - pip install pre-commit - - - name: Init terraform in the infrastructure folder for validation + - name: Init Terraform in the Infrastructure Folder for Validation run: cd infrastructure && TF_VAR_user=ci TF_VAR_stage=dev ./init_terraform.sh - - name: Init terraform in the AMI folder for validation + - name: Init Terraform in the AMI Folder for Validation run: cd ami && terraform init - - name: Run pre-commit hooks on all files - run: PATH="$PATH:$HOME/.local/bin" pre-commit run --all-files --show-diff-on-failure + - uses: pre-commit/action@v3.0.0 - test_affy_agilent: - runs-on: ubuntu-latest + # Test jobs. + test_affymetrix: + name: Test Affymetrix env: IMAGES: migrations affymetrix - DOCKER_BUILDKIT: 1 - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + needs: + - test_base + runs-on: ubuntu-latest-m steps: - uses: actions/checkout@v3 - - name: Free up space - uses: jlumbroso/free-disk-space@main - with: - # this might remove tools that are actually needed, - # if set to "true" but frees about 6 GB - tool-cache: false - - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - name: Start Postgres - run: ./scripts/run_postgres.sh + run: scripts/run_postgres.sh - - name: Pause for initialization - run: sleep 30 + - name: Pause for Initialization + run: sleep 15 - - name: Install the database - run: ./scripts/install_db_docker.sh + - name: Install Database + run: scripts/install_db_docker.sh - - name: Update the models - run: | - sudo chmod -R a+wr common - ./scripts/update_models.sh + - name: Update Models + run: scripts/update_models.sh - - name: Run affymetrix tests + - name: Run Affymetrix Tests run: | sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t affymetrix - - name: Run agilent tests - run: | - sudo -E chown -R $USER:$USER workers/test_volume - .github/scripts/filter_tests.sh -t agilent - - # This runs downloader and NO_OP tests. - downloader_no_op_tests: - runs-on: ubuntu-latest + test_agilent: + name: Test Agilent env: - # We have issues when downloading the no_op image, so for now let's just not pull it - # IMAGES: migrations api_local downloaders no_op foreman - IMAGES: migrations api_local downloaders foreman - DOCKER_BUILDKIT: 1 - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + IMAGES: migrations affymetrix + needs: + - test_base + runs-on: ubuntu-latest-m steps: - uses: actions/checkout@v3 - - name: Free up space - uses: jlumbroso/free-disk-space@main - with: - # this might remove tools that are actually needed, - # if set to "true" but frees about 6 GB - tool-cache: false - - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Pull cached docker images - run: ./.github/scripts/pull_docker_images.sh + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - name: Start Postgres - run: ./scripts/run_postgres.sh - - - name: Pause for initialization - run: sleep 30 + run: scripts/run_postgres.sh - - name: Install the database - run: ./scripts/install_db_docker.sh + - name: Pause for Initialization + run: sleep 15 - - name: Update the models - run: | - sudo chmod -R a+wr common - ./scripts/update_models.sh + - name: Install Database + run: scripts/install_db_docker.sh - - name: Run downloader tests - run: | - .github/scripts/filter_tests.sh -t downloaders + - name: Update Models + run: scripts/update_models.sh - - name: Run NO_OP tests + - name: Run Agilent Tests run: | sudo -E chown -R $USER:$USER workers/test_volume - .github/scripts/filter_tests.sh -t no_op + .github/scripts/filter_tests.sh -t agilent test_api: - runs-on: ubuntu-latest + name: Test API env: - IMAGES: migrations api_local - DOCKER_BUILDKIT: 1 - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + IMAGES: migrations api_base api_local + needs: + - check_syntax + - test_api_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Free up space - uses: jlumbroso/free-disk-space@main - with: - # this might remove tools that are actually needed, - # if set to "true" but frees about 6 GB - tool-cache: false - - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Pull cached docker images - run: ./.github/scripts/pull_docker_images.sh + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - name: Start Postgres - run: ./scripts/run_postgres.sh + run: scripts/run_postgres.sh - name: Start Elasticsearch - run: ./scripts/run_es.sh + run: scripts/run_es.sh - - name: Pause for initialization - run: sleep 30 + - name: Pause for Initialization + run: sleep 15 - - name: Install the database - run: ./scripts/install_db_docker.sh + - name: Install Database + run: scripts/install_db_docker.sh - - name: Update the models - run: | - sudo chmod -R a+wr common - ./scripts/update_models.sh + - name: Update Models + run: scripts/update_models.sh - - name: Rebuild the Elasticsearch index - run: ./scripts/rebuild_es_index.sh + - name: Rebuild the Elasticsearch Index + run: scripts/rebuild_es_index.sh - - name: Run API tests + - name: Run API Tests run: | mkdir -p test_volume sudo chmod -R a+rw test_volume - ./api/run_tests.sh + api/run_tests.sh .github/scripts/upload_test_coverage.sh api - - name: Push built docker images - run: ./.github/scripts/push_docker_images.sh - - test_common: + test_api_base: + name: Test API Base + env: + IMAGES: migrations api_base + needs: + - check_syntax runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - name: Login to Packages Container Registry + uses: docker/login-action@v2 + with: + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} + + - name: Start Postgres + run: scripts/run_postgres.sh + + - name: Start Elasticsearch + run: scripts/run_es.sh + + - name: Pause for Initialization + run: sleep 15 + + - name: Install Database + run: scripts/install_db_docker.sh + + - name: Update Models + run: scripts/update_models.sh + + - name: Build the Docker Image + run: scripts/prepare_image.sh -i api_base -s api + + test_base: + name: Test Base env: - IMAGES: migrations common_tests - DOCKER_BUILDKIT: 1 - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + IMAGES: base + needs: + - check_syntax + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Login to Packages Container registry + - name: Login to Packages Container Registry + uses: docker/login-action@v2 + with: + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} + + - name: Build the Docker Image + run: scripts/prepare_image.sh -i base -s common + + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: registry: ghcr.io username: ${{ github.actor }} password: ${{ secrets.GITHUB_TOKEN }} - - name: Pull cached docker images - run: ./.github/scripts/pull_docker_images.sh + - name: Push Built Docker Images + run: .github/scripts/push_docker_images.sh + + test_common: + name: Test Common + env: + IMAGES: migrations common_tests + needs: + - test_base + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - name: Login to Packages Container Registry + uses: docker/login-action@v2 + with: + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - name: Start Postgres - run: ./scripts/run_postgres.sh + run: scripts/run_postgres.sh - name: Start Elasticsearch - run: ./scripts/run_es.sh + run: scripts/run_es.sh - - name: Pause for initialization - run: sleep 30 + - name: Pause for Initialization + run: sleep 15 - - name: Install the database - run: ./scripts/install_db_docker.sh + - name: Install Database + run: scripts/install_db_docker.sh - - name: Update the models - run: | - sudo chmod -R a+wr common - ./scripts/update_models.sh + - name: Update Models + run: scripts/update_models.sh - - name: Rebuild the Elasticsearch index - run: ./scripts/rebuild_es_index.sh + - name: Rebuild the Elasticsearch Index + run: scripts/rebuild_es_index.sh - - name: Run Common tests + - name: Run Common Tests run: | mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume - ./common/run_tests.sh + common/run_tests.sh .github/scripts/upload_test_coverage.sh common - - name: Push built docker images - run: ./.github/scripts/push_docker_images.sh + - name: Login to Packages Container Registry + uses: docker/login-action@v2 + with: + registry: ghcr.io + username: ${{ github.actor }} + password: ${{ secrets.GITHUB_TOKEN }} + + - name: Push Built Docker Images + run: .github/scripts/push_docker_images.sh - test_foreman: - runs-on: ubuntu-latest + test_downloaders_no_op: + name: Test Downloaders and No-Op env: - IMAGES: migrations foreman - DOCKER_BUILDKIT: 1 - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + IMAGES: migrations api_base api_local downloaders foreman + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Free up space - uses: jlumbroso/free-disk-space@main + - name: Login to Packages Container Registry + uses: docker/login-action@v2 with: - # this might remove tools that are actually needed, - # if set to "true" but frees about 6 GB - tool-cache: false + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - - name: Login to Packages Container registry + - name: Start Postgres + run: scripts/run_postgres.sh + + - name: Pause for Initialization + run: sleep 15 + + - name: Install Database + run: scripts/install_db_docker.sh + + - name: Update Models + run: scripts/update_models.sh + + - name: Run Downloader Tests + run: | + .github/scripts/filter_tests.sh -t downloaders + + - name: Run No-Op Tests + run: | + sudo -E chown -R $USER:$USER workers/test_volume + .github/scripts/filter_tests.sh -t no_op + + test_foreman: + name: Test Foreman + env: + IMAGES: migrations foreman + needs: + - test_base + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v3 + + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Pull cached docker images - run: ./.github/scripts/pull_docker_images.sh + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - name: Start Postgres - run: ./scripts/run_postgres.sh + run: scripts/run_postgres.sh - name: Start Elasticsearch - run: ./scripts/run_es.sh + run: scripts/run_es.sh - - name: Pause for initialization - run: sleep 30 + - name: Pause for Initialization + run: sleep 15 - - name: Install the database - run: ./scripts/install_db_docker.sh + - name: Install Database + run: scripts/install_db_docker.sh - - name: Update the models - run: | - sudo chmod -R a+wr common - ./scripts/update_models.sh + - name: Update Models + run: scripts/update_models.sh - - name: Rebuild the Elasticsearch index - run: ./scripts/rebuild_es_index.sh + - name: Rebuild the Elasticsearch Index + run: scripts/rebuild_es_index.sh - - name: Run Foreman tests + - name: Run Foreman Tests run: | mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume - ./foreman/run_tests.sh --exclude-tag=end_to_end + foreman/run_tests.sh --exclude-tag=end_to_end .github/scripts/upload_test_coverage.sh foreman - - name: Push built docker images - run: ./.github/scripts/push_docker_images.sh + - name: Login to Packages Container Registry + uses: docker/login-action@v2 + with: + registry: ghcr.io + username: ${{ github.actor }} + password: ${{ secrets.GITHUB_TOKEN }} + + - name: Push Built Docker Images + run: .github/scripts/push_docker_images.sh test_illumina: - runs-on: ubuntu-latest + name: Test Illumina env: IMAGES: migrations illumina - DOCKER_BUILDKIT: 1 - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Pull cached docker images - run: ./.github/scripts/pull_docker_images.sh + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - name: Start Postgres - run: ./scripts/run_postgres.sh + run: scripts/run_postgres.sh - - name: Pause for initialization - run: sleep 30 + - name: Pause for Initialization + run: sleep 15 - - name: Install the database - run: ./scripts/install_db_docker.sh + - name: Install Database + run: scripts/install_db_docker.sh - - name: Update the models - run: | - sudo chmod -R a+wr common - ./scripts/update_models.sh + - name: Update Models + run: scripts/update_models.sh - - name: Run Illumina tests + - name: Run Illumina Tests run: | mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t illumina .github/scripts/upload_test_coverage.sh workers - - name: Push built docker images - run: ./.github/scripts/push_docker_images.sh + - name: Login to Packages Container Registry + uses: docker/login-action@v2 + with: + registry: ghcr.io + username: ${{ github.actor }} + password: ${{ secrets.GITHUB_TOKEN }} + + - name: Push Built Docker Images + run: .github/scripts/push_docker_images.sh test_salmon: - runs-on: ubuntu-latest + name: Test Salmon env: IMAGES: migrations salmon - DOCKER_BUILDKIT: 1 - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Pull cached docker images - run: ./.github/scripts/pull_docker_images.sh + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - name: Start Postgres - run: ./scripts/run_postgres.sh + run: scripts/run_postgres.sh - - name: Pause for initialization - run: sleep 30 + - name: Pause for Initialization + run: sleep 15 - - name: Install the database - run: ./scripts/install_db_docker.sh + - name: Install Database + run: scripts/install_db_docker.sh - - name: Update the models - run: | - sudo chmod -R a+wr common - ./scripts/update_models.sh + - name: Update Models + run: scripts/update_models.sh - - name: Run Salmon tests + - name: Run Salmon Tests run: | sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t salmon .github/scripts/upload_test_coverage.sh workers - - name: Push built docker images - run: ./.github/scripts/push_docker_images.sh + - name: Login to Packages Container Registry + uses: docker/login-action@v2 + with: + registry: ghcr.io + username: ${{ github.actor }} + password: ${{ secrets.GITHUB_TOKEN }} + + - name: Push Built Docker Images + run: .github/scripts/push_docker_images.sh test_smasher: - runs-on: ubuntu-latest + name: Test Smasher env: IMAGES: migrations smasher - DOCKER_BUILDKIT: 1 - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Free up space - uses: jlumbroso/free-disk-space@main - with: - # this might remove tools that are actually needed, - # if set to "true" but frees about 6 GB - tool-cache: false - - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Pull cached docker images - run: ./.github/scripts/pull_docker_images.sh + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - name: Start Postgres - run: ./scripts/run_postgres.sh + run: scripts/run_postgres.sh - - name: Pause for initialization - run: sleep 30 + - name: Pause for Initialization + run: sleep 15 - - name: Install the database - run: ./scripts/install_db_docker.sh + - name: Install Database + run: scripts/install_db_docker.sh - - name: Update the models - run: | - sudo chmod -R a+wr common - ./scripts/update_models.sh + - name: Update Models + run: scripts/update_models.sh - - name: Run smasher tests + - name: Run Smasher Tests run: | mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t smasher .github/scripts/upload_test_coverage.sh workers - - name: Run janitor tests + - name: Run Janitor Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t janitor .github/scripts/upload_test_coverage.sh workers - - name: Run compendia tests + - name: Run Compendia Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t compendia .github/scripts/upload_test_coverage.sh workers - - name: Run QN tests + - name: Run QN Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t qn .github/scripts/upload_test_coverage.sh workers - - name: Push built docker images - run: ./.github/scripts/push_docker_images.sh + - name: Login to Packages Container Registry + uses: docker/login-action@v2 + with: + registry: ghcr.io + username: ${{ github.actor }} + password: ${{ secrets.GITHUB_TOKEN }} + + - name: Push Built Docker Images + run: .github/scripts/push_docker_images.sh test_transcriptome: - runs-on: ubuntu-latest + name: Test Transcriptome env: - IMAGES: migrations transcriptome DOCKER_BUILDKIT: 1 - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} + IMAGES: migrations transcriptome + needs: + - test_base + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 - - name: Login to Packages Container registry + - name: Login to Packages Container Registry uses: docker/login-action@v2 with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Pull cached docker images - run: ./.github/scripts/pull_docker_images.sh + username: ${{ secrets.DOCKER_IO_USERNAME }} + password: ${{ secrets.DOCKER_IO_PASSWORD }} - name: Start Postgres - run: ./scripts/run_postgres.sh + run: scripts/run_postgres.sh - - name: Pause for initialization - run: sleep 30 + - name: Pause for Initialization + run: sleep 15 - - name: Install the database - run: ./scripts/install_db_docker.sh + - name: Install Database + run: scripts/install_db_docker.sh - - name: Update the models - run: | - sudo chmod -R a+wr common - ./scripts/update_models.sh + - name: Update Models + run: scripts/update_models.sh - - name: Run Transcriptome tests + - name: Run Transcriptome Tests run: | mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t transcriptome .github/scripts/upload_test_coverage.sh workers - - name: Push built docker images - run: ./.github/scripts/push_docker_images.sh - - determine_branch: - # As far as I can tell, this is the only way to use the output of - # a script in a github conditional. - if: startsWith(github.ref, 'refs/tags/v') - runs-on: ubuntu-latest - outputs: - branch: ${{ steps.set_branch.outputs.branch }} - steps: - - uses: actions/checkout@v3 + - name: Login to Packages Container Registry + uses: docker/login-action@v2 with: - fetch-depth: 0 + registry: ghcr.io + username: ${{ github.actor }} + password: ${{ secrets.GITHUB_TOKEN }} - - id: set_branch - name: Set the $CI_TAG environment variable - run: | - source scripts/common.sh - echo "branch=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_OUTPUT + - name: Push Built Docker Images + run: .github/scripts/push_docker_images.sh + # Deploy jobs. deploy: - if: startsWith(github.ref, 'refs/tags/v') && ! endsWith(github.ref, '-hotfix') - runs-on: ubuntu-latest - timeout-minutes: 1440 + name: Deploy env: DEPLOY_IP_ADDRESS: ${{ secrets.DEPLOY_IP_ADDRESS }} DOCKER_ID: ${{ secrets.DOCKER_ID }} @@ -526,31 +565,33 @@ jobs: PROD_DJANGO_SECRET_KEY: ${{ secrets.PROD_DJANGO_SECRET_KEY }} PROD_RAVEN_DSN: ${{ secrets.PROD_RAVEN_DSN }} PROD_RAVEN_DSN_API: ${{ secrets.PROD_RAVEN_DSN_API }} + if: startsWith(github.ref, 'refs/tags/v') && ! endsWith(github.ref, '-hotfix') needs: - determine_branch - - downloader_no_op_tests - - syntax_test - - test_affy_agilent + - test_affymetrix + - test_agilent - test_api - test_common + - test_downloaders_no_op - test_foreman - test_illumina - test_salmon - test_smasher - test_transcriptome + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 with: fetch-depth: 0 - - name: Set the $MASTER_OR_DEV and $CI_TAG environment variables + - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables run: | source scripts/common.sh echo "MASTER_OR_DEV=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV - - name: Set staging specific environment variables + - name: Set Staging Specific Environment Variables if: ${{needs.determine_branch.outputs.branch == 'dev'}} run: | echo "DATABASE_PASSWORD=${STAGING_DATABASE_PASSWORD}" >> $GITHUB_ENV @@ -560,7 +601,7 @@ jobs: # Staging doesn't notify the engagementbot, so overwrite this to be blank. echo "ENGAGEMENTBOT_WEBHOOK=" >> $GITHUB_ENV - - name: Set prod specific environment variables + - name: Set Production Specific Environment Variables if: ${{needs.determine_branch.outputs.branch == 'master'}} run: | echo "DATABASE_PASSWORD=${PROD_DATABASE_PASSWORD}" >> $GITHUB_ENV @@ -570,15 +611,14 @@ jobs: echo "ENGAGEMENTBOT_WEBHOOK=${ENGAGEMENTBOT_WEBHOOK}" >> $GITHUB_ENV - name: Deploy - run: ./.github/scripts/remote_deploy.sh + run: .github/scripts/remote_deploy.sh - - name: Cleanup deploy - run: ./.github/scripts/post_deploy_cleanup.sh - - hotfix_deploy: - if: startsWith(github.ref, 'refs/tags/v') && endsWith(github.ref, '-hotfix') - runs-on: ubuntu-latest + - name: Cleanup Deploy + run: .github/scripts/post_deploy_cleanup.sh timeout-minutes: 1440 + + deploy_hotfix: + name: Deploy Hotfix env: DEPLOY_IP_ADDRESS: ${{ secrets.DEPLOY_IP_ADDRESS }} DOCKER_ID: ${{ secrets.DOCKER_ID }} @@ -597,21 +637,23 @@ jobs: PROD_DJANGO_SECRET_KEY: ${{ secrets.PROD_DJANGO_SECRET_KEY }} PROD_RAVEN_DSN: ${{ secrets.PROD_RAVEN_DSN }} PROD_RAVEN_DSN_API: ${{ secrets.PROD_RAVEN_DSN_API }} + if: startsWith(github.ref, 'refs/tags/v') && endsWith(github.ref, '-hotfix') needs: - determine_branch + runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 with: fetch-depth: 0 - - name: Set the $MASTER_OR_DEV and $CI_TAG environment variables + - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables run: | source scripts/common.sh echo "MASTER_OR_DEV=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV - - name: Set staging specific environment variables + - name: Set Staging Specific Environment Variables if: ${{needs.determine_branch.outputs.branch == 'dev'}} run: | echo "DATABASE_PASSWORD=${STAGING_DATABASE_PASSWORD}" >> $GITHUB_ENV @@ -621,7 +663,7 @@ jobs: # Staging doesn't notify the engagementbot, so overwrite this to be blank. echo "ENGAGEMENTBOT_WEBHOOK=" >> $GITHUB_ENV - - name: Set prod specific environment variables + - name: Set Production Specific Environment Variables if: ${{needs.determine_branch.outputs.branch == 'master'}} run: | echo "DATABASE_PASSWORD=${PROD_DATABASE_PASSWORD}" >> $GITHUB_ENV @@ -631,7 +673,27 @@ jobs: echo "ENGAGEMENTBOT_WEBHOOK=${ENGAGEMENTBOT_WEBHOOK}" >> $GITHUB_ENV - name: Deploy - run: ./.github/scripts/remote_deploy.sh + run: .github/scripts/remote_deploy.sh + + - name: Cleanup Deploy + run: .github/scripts/post_deploy_cleanup.sh + timeout-minutes: 1440 + + determine_branch: + name: Determene Branch + # As far as I can tell, this is the only way to use the output of + # a script in a github conditional. + if: startsWith(github.ref, 'refs/tags/v') + runs-on: ubuntu-latest + outputs: + branch: ${{ steps.set_branch.outputs.branch }} + steps: + - uses: actions/checkout@v3 + with: + fetch-depth: 0 - - name: Cleanup deploy - run: ./.github/scripts/post_deploy_cleanup.sh + - id: set_branch + name: Set the $CI_TAG Environment Variable + run: | + source scripts/common.sh + echo "branch=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_OUTPUT diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index b651ce24a..381082dd2 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,7 +1,6 @@ --- exclude: '^(.*\.pem|.*\.tfvars)$' - repos: - repo: https://github.com/pre-commit/pre-commit-hooks rev: v2.4.0 @@ -33,18 +32,10 @@ repos: - id: shell-lint # We didn't write codecov.sh, we just get it from their servers # The other one is a template. - exclude: "codecov.sh|infrastructure/workers-configuration/workers-instance-user-data.tpl.sh" + exclude: 'codecov.sh|infrastructure/workers-configuration/workers-instance-user-data.tpl.sh' args: - # SC2154: Exclude warnings about variables being used without being - # assigned, because we sometimes use lowercase environment - # variables which shellcheck does not recognize. - # SC1090,SC1091: Don't try to follow sourced files + # SC2154: Exclude warnings about variables being used without being + # assigned, because we sometimes use lowercase environment + # variables which shellcheck does not recognize. + # SC1090,SC1091: Don't try to follow sourced files - --exclude=SC2154,SC1090,SC1091 - - - repo: https://github.com/antonbabenko/pre-commit-terraform - rev: v1.45.0 - hooks: - - id: terraform_validate - # It's a small file that gets copied up temporarily during deploys. - # Because it's not where it gets run it seems to be in a module when it's not. - exclude: "infrastructure/deploy/ci_ingress.tf" diff --git a/README.md b/README.md index 35505fbfb..ec2ac8c4a 100644 --- a/README.md +++ b/README.md @@ -19,46 +19,49 @@ Refine.bio currently has four sub-projects contained within this repo: ## Table of Contents -- [Development](#development) - - [Git Workflow](#git-workflow) - - [Installation](#installation) - - [Automatic](#automatic) - - [Linux (Manual)](#linux-manual) - - [Mac (Manual)](#mac-manual) - - [Virtual Environment](#virtual-environment) - - [Services](#services) - - [Postgres](#postgres) - - [Common Dependecies](#common-dependecies) - - [ElasticSearch](#elasticsearch) - - [Testing](#testing) - - [API](#api) - - [Common](#common) - - [Foreman](#foreman) - - [Workers](#workers) - - [Style](#style) - - [Gotchas](#gotchas) - - [R](#r) -- [Running Locally](#running-locally) - - [API](#api-1) - - [Surveyor Jobs](#surveyor-jobs) - - [Sequence Read Archive](#sequence-read-archive) - - [Ensembl Transcriptome Indices](#ensembl-transcriptome-indices) - - [Downloader Jobs](#downloader-jobs) - - [Processor Jobs](#processor-jobs) - - [Creating Quantile Normalization Reference Targets](#creating-quantile-normalization-reference-targets) - - [Creating Compendia](#creating-compendia) - - [Running Tximport Early](#running-tximport-early) - - [Development Helpers](#development-helpers) -- [Cloud Deployment](#cloud-deployment) - - [Docker Images](#docker-images) - - [Terraform](#terraform) - - [Running Jobs](#running-jobs) - - [Log Consumption](#log-consumption) - - [Dumping and Restoring Database Backups](#dumping-and-restoring-database-backups) - - [Tearing Down](#tearing-down) -- [Support](#support) -- [Meta-README](#meta-readme) -- [License](#license) +- [Refine.bio ](#refinebio--) + - [Table of Contents](#table-of-contents) + - [Development](#development) + - [Git Workflow](#git-workflow) + - [Installation](#installation) + - [Automatic](#automatic) + - [Linux (Manual)](#linux-manual) + - [Mac (Manual)](#mac-manual) + - [Virtual Environment](#virtual-environment) + - [Services](#services) + - [Postgres](#postgres) + - [Common Dependecies](#common-dependecies) + - [ElasticSearch](#elasticsearch) + - [Testing](#testing) + - [API](#api) + - [Common](#common) + - [Foreman](#foreman) + - [Workers](#workers) + - [Style](#style) + - [Gotchas](#gotchas) + - [R](#r) + - [Running Locally](#running-locally) + - [API](#api-1) + - [Surveyor Jobs](#surveyor-jobs) + - [Sequence Read Archive](#sequence-read-archive) + - [Ensembl Transcriptome Indices](#ensembl-transcriptome-indices) + - [Downloader Jobs](#downloader-jobs) + - [Processor Jobs](#processor-jobs) + - [Creating Quantile Normalization Reference Targets](#creating-quantile-normalization-reference-targets) + - [Creating Compendia](#creating-compendia) + - [Running Tximport Early](#running-tximport-early) + - [Development Helpers](#development-helpers) + - [Cloud Deployment](#cloud-deployment) + - [Docker Images](#docker-images) + - [Terraform](#terraform) + - [AWS Batch](#aws-batch) + - [Running Jobs](#running-jobs) + - [Log Consumption](#log-consumption) + - [Dumping and Restoring Database Backups](#dumping-and-restoring-database-backups) + - [Tearing Down](#tearing-down) + - [Support](#support) + - [Meta-README](#meta-readme) + - [License](#license) @@ -630,17 +633,17 @@ Please try to keep the `dev` and `master` versions in sync for major and minor v Refine.bio uses a number of different Docker images to run different pieces of the system. By default, refine.bio will pull images from the Dockerhub repo `ccdlstaging`. -If you would like to use images you have built and pushed to Dockerhub yourself you can pass the `-d` option to the `deploy.sh` script. +If you would like to use images you have built and pushed to Dockerhub yourself you can pass the `-r` option to the `deploy.sh` script. -To make building and pushing your own images easier, the `scripts/update_my_docker_images.sh` has been provided. -The `-d` option will allow you to specify which repo you'd like to push to. +To make building and pushing your own images easier, the `scripts/update_docker_images.sh` has been provided. +The `-r` option will allow you to specify which repo you'd like to push to. If the Dockerhub repo requires you to be logged in, you should do so before running the script using `docker login`. The -v option allows you to specify the version, which will both end up on the Docker images you're building as the SYSTEM_VERSION environment variable and also will be the docker tag for the image. -`scripts/update_my_docker_images.sh` will not build the dr_affymetrix image, because this image requires a lot of resources and time to build. -It can instead be built with `./scripts/prepare_image.sh -i affymetrix -d `. +`scripts/update_docker_images.sh` will not build the dr_affymetrix image, because this image requires a lot of resources and time to build. +It can instead be built with `./scripts/prepare_image.sh -i affymetrix -r `. WARNING: The affymetrix image installs a lot of data-as-R-packages and needs a lot of disk space to build the image. -It's not recommended to build the image with less than 60GB of free space on the disk that Docker runs on. +It's not recommended to build the image with less than 75GB of free space on the disk that Docker runs on. ### Terraform @@ -667,7 +670,7 @@ The correct way to deploy to the cloud is by running the `deploy.sh` script. Thi configuration steps, such as setting environment variables, setting up Batch job specifications, and performing database migrations. It can be used from the `infrastructure` directory like so: ```bash -./deploy.sh -u myusername -e dev -r us-east-1 -v v1.0.0 -d my-dockerhub-repo +./deploy.sh -u myusername -e dev -d us-east-1 -v v1.0.0 -r my-dockerhub-repo ``` This will spin up the whole system. It will usually take about 15 minutes, most of which is spent waiting for the Postgres instance to start. @@ -811,7 +814,7 @@ This can take a long time (>30 minutes)! ### Tearing Down -A stack that has been spun up via `deploy.sh -u myusername -e dev` can be taken down with `destroy_terraform.sh -u myusername -e dev -r us-east-1`. +A stack that has been spun up via `deploy.sh -u myusername -e dev` can be taken down with `destroy_terraform.sh -u myusername -e dev -d us-east-1`. The same username and environment must be passed into `destroy_terraform.sh` as were used to run `deploy.sh` either via the -e and -u options or by specifying `TF_VAR_stage` or `TF_VAR_user` so that the script knows which to take down. Note that this will prompt you for confirmation before actually destroying all of your cloud resources. diff --git a/api/dockerfiles/Dockerfile.api_base b/api/dockerfiles/Dockerfile.api_base new file mode 100644 index 000000000..d1d751104 --- /dev/null +++ b/api/dockerfiles/Dockerfile.api_base @@ -0,0 +1,29 @@ +FROM python:3.8.5-buster + +# Fail in case of an error at any stage in the pipe. +SHELL ["/bin/bash", "-o", "pipefail", "-c"] + +# Install gcc and libpq-dev for psycopg2. +RUN apt-get update -qq && \ + apt-get install -y \ + gcc \ + libpq-dev && \ + groupadd user && \ + useradd --create-home --home-dir /home/user -g user user + +WORKDIR /home/user + +COPY api/requirements.txt . +RUN pip install --upgrade pip && \ + pip install --ignore-installed --no-cache-dir -r requirements.txt + +COPY common/dist/data-refinery-common-* common/ +RUN pip install --ignore-installed \ + common/$(ls common -1 | sort --version-sort | tail -1) + +COPY api/ . +COPY config/ config/ + +ENV SYSTEM_VERSION=$SYSTEM_VERSION + +ENTRYPOINT [] diff --git a/api/dockerfiles/Dockerfile.api_local b/api/dockerfiles/Dockerfile.api_local index 2018cd59b..38cd1e5b7 100644 --- a/api/dockerfiles/Dockerfile.api_local +++ b/api/dockerfiles/Dockerfile.api_local @@ -1,35 +1,10 @@ -FROM python:3.8.5-slim-buster +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_api_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Install gcc and libpq-dev for psycopg2. -RUN apt-get update -qq && \ - apt-get install -y \ - gcc \ - libpq-dev - -RUN groupadd user && \ - useradd --create-home --home-dir /home/user -g user user -WORKDIR /home/user - -COPY api/requirements.txt . -RUN pip install --upgrade pip && \ - pip install --ignore-installed -r requirements.txt && \ - rm -r /root/.cache - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) - -COPY api/ . -COPY config/ config/ -COPY setup.cfg . - -ARG SYSTEM_VERSION -ENV SYSTEM_VERSION=$SYSTEM_VERSION - USER user EXPOSE 8000 diff --git a/api/dockerfiles/Dockerfile.api_production b/api/dockerfiles/Dockerfile.api_production index 37184be03..fccc67d0f 100644 --- a/api/dockerfiles/Dockerfile.api_production +++ b/api/dockerfiles/Dockerfile.api_production @@ -1,39 +1,14 @@ -FROM python:3.8.5-buster +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_api_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] -# Install gcc and libpq-dev for psycopg2. -RUN apt-get update -qq && \ - apt-get install -y \ - gcc \ - libpq-dev - -RUN groupadd user && \ - useradd --create-home --home-dir /home/user -g user user -WORKDIR /home/user - -COPY api/requirements.txt . -RUN pip install --upgrade pip && \ - pip install --ignore-installed -r requirements.txt && \ - pip install --ignore-installed uwsgi && \ - rm -r /root/.cache - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip install --ignore-installed \ - common/$(ls common -1 | sort --version-sort | tail -1) - -COPY api/ . -COPY config/ config/ - RUN chmod +x /home/user/collect_and_run_uwsgi.sh && \ mkdir -p /tmp/www/static && \ chown user /tmp/www/static -ARG SYSTEM_VERSION -ENV SYSTEM_VERSION=$SYSTEM_VERSION - USER user # We collect Django's static files and expose them as a volume so that Nginx diff --git a/api/run_tests.sh b/api/run_tests.sh index f81309fbd..f4353c679 100755 --- a/api/run_tests.sh +++ b/api/run_tests.sh @@ -4,27 +4,31 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. -# Ensure that postgres is running +# Ensure that Postgres is running. if ! [ "$(docker ps --filter name=drdb -q)" ]; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 exit 1 fi -# Ensure that elasticsearch is running + +# Ensure that ElasticSearch is running. if ! [ "$(docker ps --filter name=dres -q)" ]; then echo "You must start elasticsearchfirst with:" >&2 echo "./scripts/run_es.sh" >&2 exit 1 fi -project_root=$(pwd) # "cd .." called above +project_root=$(pwd) # "cd .." called above. volume_directory="$project_root/test_volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" @@ -34,18 +38,23 @@ chmod -R a+rwX "$volume_directory" ./scripts/prepare_image.sh -i api_local -s api . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) ES_HOST_IP=$(get_docker_es_ip_address) -# Only run interactively if we are on a TTY +# Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi -docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --add-host=elasticsearch:"$ES_HOST_IP" \ - --env-file api/environments/test \ - --platform linux/amd64 \ - --volume "$volume_directory":/home/user/data_store \ - ccdlstaging/dr_api_local bash -c "$(run_tests_with_coverage "$@")" +# shellcheck disable=SC2086 +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --add-host=elasticsearch:"$ES_HOST_IP" \ + --env-file api/environments/test \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + $INTERACTIVE \ + "$DOCKERHUB_REPO/dr_api_local" \ + bash -c "$(run_tests_with_coverage "$@")" diff --git a/api/serve.sh b/api/serve.sh index 4bae59fe9..1cd32a622 100755 --- a/api/serve.sh +++ b/api/serve.sh @@ -4,22 +4,29 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. ./scripts/prepare_image.sh -i api_local -s api . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) ES_HOST_IP=$(get_docker_es_ip_address) docker run \ - --add-host=database:"$DB_HOST_IP" \ - --add-host=elasticsearch:"$ES_HOST_IP" \ - --env-file api/environments/local \ - -p 8000:8000 \ - -it ccdlstaging/dr_api_local python3 manage.py runserver 0.0.0.0:8000 "$@" + --add-host=database:"$DB_HOST_IP" \ + --add-host=elasticsearch:"$ES_HOST_IP" \ + --env-file api/environments/local \ + --interactive \ + --publish 8000:8000 \ + --tty \ + "$DOCKERHUB_REPO/dr_api_local" \ + python3 manage.py runserver 0.0.0.0:8000 "$@" diff --git a/api/serve_production.sh b/api/serve_production.sh index 530b237ca..c23382778 100755 --- a/api/serve_production.sh +++ b/api/serve_production.sh @@ -4,24 +4,31 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. ./scripts/prepare_image.sh -i api_production -s api . ./scripts/common.sh -DB_HOST_IP=$(get_docker_db_ip_address) +DB_HOST_IP=$(get_docker_db_ip_address) STATIC_VOLUMES=/tmp/volumes_static docker run \ - --add-host=database:"$DB_HOST_IP" \ - --env-file api/environments/local \ - --link drdb:postgres \ - -v "$STATIC_VOLUMES":/tmp/www/static \ - -p 8081:8081 \ - -it -d ccdlstaging/dr_api_production /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" + --add-host=database:"$DB_HOST_IP" \ + --detach \ + --env-file api/environments/local \ + --interactive \ + --link drdb:postgres \ + --publish 8081:8081 \ + --tty \ + --volume "$STATIC_VOLUMES":/tmp/www/static \ + "$DOCKERHUB_REPO/dr_api_production" \ + /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" diff --git a/common/R/renv_load.R b/common/R/renv_load.R index abaf13935..dd5caa080 100644 --- a/common/R/renv_load.R +++ b/common/R/renv_load.R @@ -1,13 +1,16 @@ options(warn = 2) options(repos = structure(c( - Bioconductor = "https://bioconductor.org/packages/3.11/bioc/", - BioconductorAnnotation = "https://bioconductor.org/packages/3.11/data/annotation/", - BioconductorExperiment = "https://bioconductor.org/packages/3.11/data/experiment", - CRAN = "https://cloud.r-project.org/" + Bioconductor = "https://bioconductor.org/packages/3.6/bioc", + BioconductorAnnotation = "https://bioconductor.org/packages/3.6/data/annotation", + BioconductorExperiment = "https://bioconductor.org/packages/3.6/data/experiment", + CRAN = "https://cloud.r-project.org" ))) options(Ncpus = parallel::detectCores()) +options(renv.r.version = "3.4.4") +options(renv.settings.use.cache = FALSE) -install.packages("renv") +install.packages("BiocInstaller") +install.packages("https://cran.r-project.org/src/contrib/Archive/renv/renv_0.16.0.tar.gz") renv::consent(provided = TRUE) -renv::restore(prompt = FALSE) +renv::restore(prompt = FALSE, rebuild = TRUE) diff --git a/workers/R/renv_save.R b/common/R/renv_save.R similarity index 100% rename from workers/R/renv_save.R rename to common/R/renv_save.R diff --git a/common/dockerfiles/Dockerfile.common_tests b/common/dockerfiles/Dockerfile.common_tests index 2a31940f4..26b2be946 100644 --- a/common/dockerfiles/Dockerfile.common_tests +++ b/common/dockerfiles/Dockerfile.common_tests @@ -1,4 +1,6 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] @@ -15,7 +17,6 @@ RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt COPY common/ . COPY config config -ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/common/dockerfiles/Dockerfile.migrations b/common/dockerfiles/Dockerfile.migrations index 00e704fa2..da2405265 100644 --- a/common/dockerfiles/Dockerfile.migrations +++ b/common/dockerfiles/Dockerfile.migrations @@ -1,4 +1,6 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] @@ -11,7 +13,6 @@ RUN pip install --ignore-installed --no-cache-dir -r requirements.txt COPY common/ . COPY config config -ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/common/run_tests.sh b/common/run_tests.sh index 51c564270..41eb8e3d1 100755 --- a/common/run_tests.sh +++ b/common/run_tests.sh @@ -1,24 +1,27 @@ #!/bin/sh -e -# script for executing Django PyUnit Tests within a Docker container. +# Script for executing Django PyUnit tests within a Docker container. # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. -# Ensure that postgres is running +# Ensure that Postgres is running. if ! [ "$(docker ps --filter name=drdb -q)" ]; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 exit 1 fi -project_root=$(pwd) # "cd .." called above +project_root=$(pwd) # "cd .." called above. volume_directory="$project_root/test_volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" @@ -28,18 +31,23 @@ chmod -R a+rwX "$volume_directory" ./scripts/prepare_image.sh -i common_tests -s common . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) ES_HOST_IP=$(get_docker_es_ip_address) -# Only run interactively if we are on a TTY +# Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi -docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --add-host=elasticsearch:"$ES_HOST_IP" \ - --env-file common/environments/test \ - --platform linux/amd64 \ - --volume "$volume_directory":/home/user/data_store \ - ccdlstaging/dr_common_tests bash -c "$(run_tests_with_coverage "$@")" --parallel +# shellcheck disable=SC2086 +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --add-host=elasticsearch:"$ES_HOST_IP" \ + --env-file common/environments/test \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + $INTERACTIVE \ + "$DOCKERHUB_REPO/dr_common_tests" \ + bash -c "$(run_tests_with_coverage "$@")" --parallel diff --git a/common/setup.py b/common/setup.py index 78339b2e1..4234b9ab5 100644 --- a/common/setup.py +++ b/common/setup.py @@ -1,4 +1,6 @@ import os +import re +from datetime import datetime from setuptools import find_packages, setup @@ -11,11 +13,21 @@ version_string = version_file.read().strip().split("-")[0] except OSError: print( - "Cannot read version to determine System Version." - " Please create a file common/version containing an up to date System Version." + "Cannot read version file to determine system version. " + "Please create a file common/version containing an up to date system version." ) raise +version_re = re.compile( + r"^([1-9][0-9]*!)?(0|[1-9][0-9]*)" + "(\.(0|[1-9][0-9]*))*((a|b|rc)(0|[1-9][0-9]*))" + "?(\.post(0|[1-9][0-9]*))?(\.dev(0|[1-9][0-9]*))?$" +) +if not version_re.match(version_string): + # Generate version based on the datetime.now(): e.g., 2023.5.17.dev1684352560. + now = datetime.now() + version_string = f"{now.strftime('%Y.%-m.%-d.dev')}{int(datetime.timestamp(now))}" + setup( name="data-refinery-common", version=version_string, diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 94e5c185a..89e3611f0 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -1,16 +1,19 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -ADD https://gbnci.cancer.gov/geo/GEOmetadb.sqlite.gz data/microarray/GEOmetadb.sqlite.gz -RUN gunzip data/microarray/GEOmetadb.sqlite.gz && \ - chmod 644 data/microarray/GEOmetadb.sqlite - COPY foreman/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt +RUN mkdir -p data/microarray && \ + wget -q https://gbnci.cancer.gov/geo/GEOmetadb.sqlite.gz \ + -O data/microarray/GEOmetadb.sqlite.gz && \ + gunzip data/microarray/GEOmetadb.sqlite.gz && \ + chmod 644 data/microarray/GEOmetadb.sqlite && \ + pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ @@ -21,7 +24,6 @@ COPY .boto .boto COPY config config COPY foreman/ . -ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/foreman/run_end_to_end_tests.sh b/foreman/run_end_to_end_tests.sh index 554068980..e08112ae2 100755 --- a/foreman/run_end_to_end_tests.sh +++ b/foreman/run_end_to_end_tests.sh @@ -4,7 +4,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Set up the data volume directory if it does not already exist. @@ -25,30 +28,27 @@ if [ ! -e "$reference_file_dir/$quant_file" ]; then mkdir -p "$reference_file_dir" echo "Downloading quant file for Transcriptome Index validation tests." wget -q -O "$reference_file_dir/$quant_file" \ - "$test_data_repo/$quant_file" + "$test_data_repo/$quant_file" fi # temp for testing locally. ../scripts/prepare_image.sh -i foreman -s foreman - while read -r row; do # Exporting an expansion rather than a variable, which is exactly what we want to do. # shellcheck disable=SC2163 export "${row}" -done < ../infrastructure/prod_env - +done <../infrastructure/prod_env -# Hardcode ccdlstaging because this should only ever be run in staging or -# locally, and when running locally `prepare_image.sh` makes the forman -# ccdlstaging/dr_foreman. -docker run -t \ - --env-file ../infrastructure/prod_env \ - --env RUNNING_IN_CLOUD=False \ +docker run \ --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ + --env DJANGO_SECRET_KEY="TEST_KEY_FOR_DEV" \ --env JOB_DEFINITION_PREFIX="$USER_$STAGE_" \ --env REFINEBIO_BASE_URL="http://$API_HOST/v1/" \ - --env DJANGO_SECRET_KEY="TEST_KEY_FOR_DEV" \ - --volume "$volume_directory":/home/user/data_store \ + --env RUNNING_IN_CLOUD=False \ + --env-file ../infrastructure/prod_env \ --volume "$HOME/.aws":/home/user/.aws \ - ccdlstaging/dr_foreman python3 manage.py test --no-input --parallel=2 --testrunner='tests.test_runner.NoDbTestRunner' tests.foreman.test_end_to_end + --volume "$volume_directory":/home/user/data_store \ + --tty \ + "$DOCKERHUB_REPO/dr_foreman" \ + python3 manage.py test --no-input --parallel=2 --testrunner='tests.test_runner.NoDbTestRunner' tests.foreman.test_end_to_end diff --git a/foreman/run_management_command.sh b/foreman/run_management_command.sh index a9e4ce101..d51768fd8 100755 --- a/foreman/run_management_command.sh +++ b/foreman/run_management_command.sh @@ -1,13 +1,16 @@ #!/bin/sh -# Script for running the Data Refinery Surveyor container +# Script for running the Data Refinery Surveyor container. # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to +# However, in order to give Docker access to all the code we have to # move up a level cd .. @@ -21,12 +24,16 @@ chmod -R a+rwX "$volume_directory" ./scripts/prepare_image.sh -i foreman -s foreman . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) -docker run -it \ - --add-host=database:"$DB_HOST_IP" \ - --env-file foreman/environments/local \ - --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ - --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ - --volume "$volume_directory":/home/user/data_store \ - ccdlstaging/dr_foreman python3 manage.py "$@" +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ + --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ + --env-file foreman/environments/local \ + --interactive \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + "$DOCKERHUB_REPO/dr_foreman" \ + python3 manage.py "$@" diff --git a/foreman/run_tests.sh b/foreman/run_tests.sh index 8edbdc56f..f0ae37de4 100755 --- a/foreman/run_tests.sh +++ b/foreman/run_tests.sh @@ -4,7 +4,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Set up the data volume directory if it does not already exist. @@ -18,11 +21,11 @@ if [ ! -d "$volume_directory" ]; then fi chmod -R a+rwX "$volume_directory" -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. -# First ensure postgres is running +# First ensure Postgres is running. if ! [ "$(docker ps --filter name=drdb -q)" ]; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 @@ -32,16 +35,21 @@ fi ./scripts/prepare_image.sh -i foreman -s foreman . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) -# Only run interactively if we are on a TTY +# Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi -docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --env-file foreman/environments/test \ - --platform linux/amd64 \ - --volume "$volume_directory":/home/user/data_store \ - ccdlstaging/dr_foreman bash -c "$(run_tests_with_coverage --exclude-tag=manual "$@")" +# shellcheck disable=SC2086 +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --env-file foreman/environments/test \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + $INTERACTIVE \ + "$DOCKERHUB_REPO/dr_foreman" \ + bash -c "$(run_tests_with_coverage --exclude-tag=manual "$@")" diff --git a/foreman/test_survey.sh b/foreman/test_survey.sh index 7df91a3ae..1aec44116 100755 --- a/foreman/test_survey.sh +++ b/foreman/test_survey.sh @@ -2,7 +2,7 @@ # Script for testing the surveying of an accession manually (e.g. from a dataset request) print_options() { - cat << EOF + cat <&2 echo "./scripts/run_postgres.sh" >&2 @@ -76,12 +79,16 @@ fi ./scripts/prepare_image.sh -i foreman -s foreman . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) docker run \ - --add-host=database:"$DB_HOST_IP" \ - --env-file foreman/environments/test \ - --volume "$volume_directory":/home/user/data_store \ - -e SURVEYOR="$SURVEYOR" \ - -e ACCESSION="$ACCESSION" \ - -it ccdlstaging/dr_foreman bash -c "python3 manage.py test --tag=manual ." + --add-host=database:"$DB_HOST_IP" \ + --env ACCESSION="$ACCESSION" \ + --env SURVEYOR="$SURVEYOR" \ + --env-file foreman/environments/test \ + --interactive \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + "$DOCKERHUB_REPO/dr_foreman" \ + bash -c "python3 manage.py test --tag=manual ." diff --git a/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh b/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh index 3f475c98b..0dfb15ddc 100644 --- a/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh +++ b/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh @@ -15,7 +15,7 @@ cd /home/ubuntu || exit # Install and configure Nginx. -cat <<"EOF" > nginx.conf +cat <<"EOF" >nginx.conf ${nginx_config} EOF apt-get update -y @@ -107,7 +107,7 @@ echo " size 20k daily maxage 3 -}" >> /etc/logrotate.conf +}" >>/etc/logrotate.conf echo " /tmp/access.log { missingok @@ -116,10 +116,10 @@ echo " size 20k daily maxage 3 -}" >> /etc/logrotate.conf +}" >>/etc/logrotate.conf # Install our environment variables -cat <<"EOF" > environment +cat <<"EOF" >environment ${api_environment} EOF @@ -135,36 +135,40 @@ docker pull "${dockerhub_repo}/${api_docker_image}" # These database values are created after TF # is run, so we have to pass them in programatically docker run \ - --env-file environment \ - -e DATABASE_HOST="${database_host}" \ - -e DATABASE_NAME="${database_name}" \ - -e DATABASE_USER="${database_user}" \ - -e DATABASE_PASSWORD="${database_password}" \ - -e ELASTICSEARCH_HOST="${elasticsearch_host}" \ - -e ELASTICSEARCH_PORT="${elasticsearch_port}" \ - -v "$STATIC_VOLUMES":/tmp/www/static \ - --log-driver=awslogs \ - --log-opt awslogs-region="${region}" \ - --log-opt awslogs-group="${log_group}" \ - --log-opt awslogs-stream="${log_stream}" \ - -p 8081:8081 \ - --name=dr_api \ - -it -d "${dockerhub_repo}/${api_docker_image}" /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" + --detach \ + --env DATABASE_HOST="${database_host}" \ + --env DATABASE_NAME="${database_name}" \ + --env DATABASE_PASSWORD="${database_password}" \ + --env DATABASE_USER="${database_user}" \ + --env ELASTICSEARCH_HOST="${elasticsearch_host}" \ + --env ELASTICSEARCH_PORT="${elasticsearch_port}" \ + --env-file environment \ + --interactive \ + --log-driver=awslogs \ + --log-opt awslogs-group="${log_group}" \ + --log-opt awslogs-region="${region}" \ + --log-opt awslogs-stream="${log_stream}" \ + --name=dr_api \ + --tty \ + --volume "$STATIC_VOLUMES":/tmp/www/static \ + -publish 8081:8081 \ + "${dockerhub_repo}/${api_docker_image}" \ + /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" # Nuke and rebuild the search index. It shouldn't take too long. sleep 30 -docker exec dr_api python3 manage.py search_index --delete -f; -docker exec dr_api python3 manage.py search_index --rebuild -f; -docker exec dr_api python3 manage.py search_index --populate -f; +docker exec dr_api python3 manage.py search_index --delete -f +docker exec dr_api python3 manage.py search_index --rebuild -f +docker exec dr_api python3 manage.py search_index --populate -f # Let's use this instance to call the populate command every twenty minutes. -crontab -l > tempcron +crontab -l >tempcron # echo new cron into cron file # TODO: stop logging this to api_cron.log once we figure out why it # hasn't been working. -echo -e "SHELL=/bin/bash\nPATH=/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin\n*/20 * * * * docker exec dr_api python3 manage.py update_es_index >> /var/log/api_cron.log 2>&1" >> tempcron +echo -e "SHELL=/bin/bash\nPATH=/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin\n*/20 * * * * docker exec dr_api python3 manage.py update_es_index >> /var/log/api_cron.log 2>&1" >>tempcron # Post a summary of downloads every Monday at 12:00 UTC -echo -e "SHELL=/bin/bash\nPATH=/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin\n0 12 * * MON docker exec dr_api python3 manage.py post_downloads_summary >> /var/log/api_cron.log 2>&1" >> tempcron +echo -e "SHELL=/bin/bash\nPATH=/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin\n0 12 * * MON docker exec dr_api python3 manage.py post_downloads_summary >> /var/log/api_cron.log 2>&1" >>tempcron # install new cron file crontab tempcron rm tempcron diff --git a/infrastructure/deploy.sh b/infrastructure/deploy.sh index d8175b423..2298c2d8a 100755 --- a/infrastructure/deploy.sh +++ b/infrastructure/deploy.sh @@ -2,7 +2,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit print_description() { @@ -21,7 +24,7 @@ print_options() { echo ' "-e prod" will deploy the production stack. This should only be used from a CD machine.' echo ' "-e staging" will deploy the staging stack. This should only be used from a CD machine.' echo ' "-e dev" will deploy a dev stack which is appropriate for a single developer to use to test.' - echo '-d May be used to override the Dockerhub repo where the images will be pulled from.' + echo '-r May be used to override the Dockerhub repo where the images will be pulled from.' echo ' This may also be specified by setting the TF_VAR_dockerhub_repo environment variable.' echo ' If unset, defaults to "ccdlstaging" if the version contains "-dev" and "ccdl" otherwise.' echo ' for dev and staging environments and "ccdl" for prod.' @@ -31,7 +34,7 @@ print_options() { echo '-v specifies the version of the system which is being deployed and is not optional.' echo "-u specifies the username of the deployer. Should be the developer's name in development stacks." echo ' This option may be omitted, in which case the TF_VAR_user variable MUST be set instead.' - echo '-r specifies the AWS region to deploy the stack to. Defaults to us-east-1.' + echo '-d specifies the AWS region to deploy the stack to. Defaults to us-east-1.' } while getopts ":e:d:i:v:u:r:h" opt; do @@ -40,7 +43,7 @@ while getopts ":e:d:i:v:u:r:h" opt; do export env=$OPTARG export TF_VAR_stage=$OPTARG ;; - d) + r) export TF_VAR_dockerhub_repo=$OPTARG ;; i) @@ -53,7 +56,7 @@ while getopts ":e:d:i:v:u:r:h" opt; do u) export TF_VAR_user=$OPTARG ;; - r) + d) export TF_VAR_region=$OPTARG ;; h) @@ -102,31 +105,29 @@ if [[ -z $TF_VAR_region ]]; then TF_VAR_region=us-east-1 fi - # We have terraform output environment variables via a single output # variable, which we then read in as json using the command line tool # `jq`, so that we can use them via bash. -format_environment_variables () { - json_env_vars=$(terraform output -json environment_variables | jq -c '.[]') - for row in $json_env_vars; do - name=$(echo "$row" | jq -r ".name") - value=$(echo "$row" | jq -r ".value") - env_var_assignment="$name=$value" - # Exporting an expansion rather than a variable, which is exactly what we want to do. - # shellcheck disable=SC2163 - export "${env_var_assignment?}" - echo "$env_var_assignment" >> prod_env - done +format_environment_variables() { + json_env_vars=$(terraform output -json environment_variables | jq -c '.[]') + for row in $json_env_vars; do + name=$(echo "$row" | jq -r ".name") + value=$(echo "$row" | jq -r ".value") + env_var_assignment="$name=$value" + # Exporting an expansion rather than a variable, which is exactly what we want to do. + # shellcheck disable=SC2163 + export "${env_var_assignment?}" + echo "$env_var_assignment" >>prod_env + done } - -# Load $ALL_CCDL_IMAGES and helper functions -source ../scripts/common.sh +# Load $ALL_IMAGES and helper functions. +. ../scripts/common.sh # Make our IP address known to terraform. TF_VAR_host_ip="$(dig +short myip.opendns.com @resolver1.opendns.com)" export TF_VAR_host_ip -for IMAGE in $ALL_CCDL_IMAGES; do +for IMAGE in $ALL_IMAGES; do # For each image we need to set the env var that is used by our # scripts and the env var that gets picked up by terraform because # it is preceeded with TF_VAR. @@ -140,27 +141,26 @@ cp deploy/ci_ingress.tf . # Check if a new ccdl-ubuntu ami will be needed for this region if [[ $(aws ec2 describe-images \ - --region $TF_VAR_region --owners 589864003899 \ - --filters 'Name=name,Values=ccdl-ubuntu-18.04-*' \ - --query 'length(Images)') \ - -eq 0 ]]; then + --region "$TF_VAR_region" --owners 589864003899 \ + --filters 'Name=name,Values=ccdl-ubuntu-18.04-*' \ + --query 'length(Images)') -eq 0 ]]; then echo "No ccdl-ubuntu-18.04 AMI found for this region, creating a new one" # Find most recent ccdl-ubuntu ami from us-east-1 template_ami_id=$(aws ec2 describe-images \ - --region us-east-1 --owners 589864003899 \ - --filters 'Name=name,Values=ccdl-ubuntu-18.04-*' \ - --query 'sort_by(Images,&CreationDate)[-1].ImageId' \ - --output text) + --region us-east-1 --owners 589864003899 \ + --filters 'Name=name,Values=ccdl-ubuntu-18.04-*' \ + --query 'sort_by(Images,&CreationDate)[-1].ImageId' \ + --output text) # Make a copy into this region new_ami_name="ccdl-ubuntu-18.04-$(date "+%Y-%m-%dT%H.%M.%S")" new_ami_id=$(aws ec2 copy-image \ - --source-image-id "$template_ami_id" \ - --source-region us-east-1 \ - --region "$TF_VAR_region" \ - --name "$new_ami_name" \ - --output text) + --source-image-id "$template_ami_id" \ + --source-region us-east-1 \ + --region "$TF_VAR_region" \ + --name "$new_ami_name" \ + --output text) echo "Created new AMI for $TF_VAR_region" echo " name: $new_ami_name" echo " id: $new_ami_id" @@ -174,16 +174,16 @@ fi terraform taint module.batch.aws_launch_template.data_refinery_worker || true terraform taint module.batch.aws_launch_template.data_refinery_compendia || true if terraform state list | grep -q module.batch.aws_batch_job_queue.data_refinery_; then - terraform state list \ - | grep module.batch.aws_batch_job_queue.data_refinery_ \ - | xargs -L 1 terraform taint \ - || true + terraform state list | + grep module.batch.aws_batch_job_queue.data_refinery_ | + xargs -L 1 terraform taint || + true fi if terraform state list | grep -q module.batch.aws_batch_compute_environment.data_refinery__; then - terraform state list \ - | grep module.batch.aws_batch_compute_environment.data_refinery_ \ - | xargs -L 1 terraform taint \ - || true + terraform state list | + grep module.batch.aws_batch_compute_environment.data_refinery_ | + xargs -L 1 terraform taint || + true fi if terraform output | grep -q 'No outputs found'; then @@ -232,7 +232,7 @@ format_environment_variables # Make sure to clear out any old batch job templates since we # will register everything in this directory. if [ -e batch-job-templates ]; then - rm -r batch-job-templates + rm -r batch-job-templates fi # Template the environment variables for production into the Batch Job @@ -272,47 +272,49 @@ docker pull "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" # Test that the pg_bouncer instance is up. 15 minutes should be more than enough. start_time=$(date +%s) diff=0 -until pg_isready -d "$DATABASE_NAME" -h "$DATABASE_PUBLIC_HOST" -p "$DATABASE_PORT" -U "$DATABASE_USER" &> /dev/null || [ "$diff" -gt "900" ] -do +until pg_isready -d "$DATABASE_NAME" -h "$DATABASE_PUBLIC_HOST" -p "$DATABASE_PORT" -U "$DATABASE_USER" &>/dev/null || [ "$diff" -gt "900" ]; do echo "Waiting for the pg_bouncer instance to come online ..." sleep 10 - (( diff = $(date +%s) - start_time )) + ((diff = $(date +%s) - start_time)) done -if ! pg_isready -d "$DATABASE_NAME" -h "$DATABASE_PUBLIC_HOST" -p "$DATABASE_PORT" -U "$DATABASE_USER" &> /dev/null; then +if ! pg_isready -d "$DATABASE_NAME" -h "$DATABASE_PUBLIC_HOST" -p "$DATABASE_PORT" -U "$DATABASE_USER" &>/dev/null; then echo "pg_bouncer instance failed to come up after 15 minutes." exit 1 fi # Migrate auth. docker run \ - --env-file prod_env \ - --env RUNNING_IN_CLOUD=False \ - --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ - "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" python3 manage.py migrate auth + --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ + --env RUNNING_IN_CLOUD=False \ + --env-file prod_env \ + "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ + python3 manage.py migrate auth # Apply general migrations. docker run \ - --env-file prod_env \ - --env RUNNING_IN_CLOUD=False \ - --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ - "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" python3 manage.py migrate + --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ + --env RUNNING_IN_CLOUD=False \ + --env-file prod_env \ + "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ + python3 manage.py migrate # Create the cache table if it does not already exist. docker run \ - --env-file prod_env \ - --env RUNNING_IN_CLOUD=False \ - --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ - "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" python3 manage.py createcachetable + --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ + --env RUNNING_IN_CLOUD=False \ + --env-file prod_env \ + "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ + python3 manage.py createcachetable # Terraform doesn't manage these well, so they need to be tainted to # ensure they won't require manual intervention. terraform taint module.batch.aws_launch_template.data_refinery_worker terraform taint module.batch.aws_launch_template.data_refinery_compendia -terraform state list \ - | grep module.batch.aws_batch_job_queue.data_refinery_ \ - | xargs -L 1 terraform taint \ - || true +terraform state list | + grep module.batch.aws_batch_job_queue.data_refinery_ | + xargs -L 1 terraform taint || + true # Ensure the latest image version is being used for the Foreman terraform taint aws_instance.foreman_server_1 @@ -339,10 +341,10 @@ API_IP_ADDRESS=$(terraform output -json api_server_1_ip | tr -d '"') # it's not found then grep will return a non-zero exit code so in that # case return an empty string. container_running=$(ssh -o StrictHostKeyChecking=no \ - -o ServerAliveInterval=15 \ - -o ConnectTimeout=5 \ - -i data-refinery-key.pem \ - "ubuntu@$API_IP_ADDRESS" "docker ps -a" 2> /dev/null | grep dr_api || echo "") + -o ServerAliveInterval=15 \ + -o ConnectTimeout=5 \ + -i data-refinery-key.pem \ + "ubuntu@$API_IP_ADDRESS" "docker ps -a" 2>/dev/null | grep dr_api || echo "") # If $container_running is empty, then it's because the container isn't running. # If the container isn't running, then it's because the instance is spinning up. @@ -377,21 +379,25 @@ if [[ -n $container_running ]]; then -o ConnectTimeout=5 \ -i data-refinery-key.pem \ "ubuntu@$API_IP_ADDRESS" "docker run \ - --env-file environment \ - -e DATABASE_HOST=$DATABASE_HOST \ - -e DATABASE_NAME=$DATABASE_NAME \ - -e DATABASE_USER=$DATABASE_USER \ - -e DATABASE_PASSWORD=$DATABASE_PASSWORD \ - -e ELASTICSEARCH_HOST=$ELASTICSEARCH_HOST \ - -e ELASTICSEARCH_PORT=$ELASTICSEARCH_PORT \ - -v /tmp/volumes_static:/tmp/www/static \ - --log-driver=awslogs \ - --log-opt awslogs-region=$AWS_REGION \ - --log-opt awslogs-group=data-refinery-log-group-$USER-$STAGE \ - --log-opt awslogs-stream=log-stream-api-$USER-$STAGE \ - -p 8081:8081 \ - --name=dr_api \ - -it -d $DOCKERHUB_REPO/$API_DOCKER_IMAGE /bin/sh -c /home/user/collect_and_run_uwsgi.sh" + --detach \ + --env DATABASE_HOST=$DATABASE_HOST \ + --env DATABASE_NAME=$DATABASE_NAME \ + --env DATABASE_PASSWORD=$DATABASE_PASSWORD \ + --env DATABASE_USER=$DATABASE_USER \ + --env ELASTICSEARCH_HOST=$ELASTICSEARCH_HOST \ + --env ELASTICSEARCH_PORT=$ELASTICSEARCH_PORT \ + --env-file environment \ + --interactive \ + --log-driver=awslogs \ + --log-opt awslogs-group=data-refinery-log-group-$USER-$STAGE \ + --log-opt awslogs-region=$AWS_REGION \ + --log-opt awslogs-stream=log-stream-api-$USER-$STAGE \ + --name=dr_api \ + --tty \ + --volume /tmp/volumes_static:/tmp/www/static \ + --publish 8081:8081 \ + $DOCKERHUB_REPO/$API_DOCKER_IMAGE \ + /bin/sh -c /home/user/collect_and_run_uwsgi.sh" # Don't leave secrets lying around. ssh -o StrictHostKeyChecking=no \ diff --git a/infrastructure/deploy_box_instance_data.sh b/infrastructure/deploy_box_instance_data.sh index 25f7bf5c7..dffe93ecf 100644 --- a/infrastructure/deploy_box_instance_data.sh +++ b/infrastructure/deploy_box_instance_data.sh @@ -76,5 +76,6 @@ chown ubuntu:ubuntu /var/log/deploy.log # Checkout the repo onto the box. cd /home/ubuntu +umask 0022 git clone https://github.com/AlexsLemonade/refinebio.git chown -R ubuntu:ubuntu refinebio diff --git a/infrastructure/destroy_terraform.sh b/infrastructure/destroy_terraform.sh index cf91053e4..4329cf885 100755 --- a/infrastructure/destroy_terraform.sh +++ b/infrastructure/destroy_terraform.sh @@ -2,7 +2,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit print_description() { @@ -10,17 +13,17 @@ print_description() { } print_options() { - echo 'This script accepts the following arguments: -e, -u, -r, and -h.' - echo 'Neither -e, -u or -r is optional unless TF_VAR_stage, TF_VAR_user,' + echo 'This script accepts the following arguments: -e, -u, -d, and -h.' + echo 'Neither -e, -u or -d is optional unless TF_VAR_stage, TF_VAR_user,' echo 'or TF_VAR_region is set, respectively.' echo '-h prints this help message and exits.' echo '-e specifies the environment you would like to destroy.' echo '-u specifies the username you used to spin up the stack.' - echo '-r specifies the region of the stack to destroy.' + echo '-d specifies the region of the stack to destroy.' echo 'All arguments are needed to determine which stack to destroy.' } -while getopts ":e:u:r:h" opt; do +while getopts ":e:u:d:h" opt; do case $opt in e) export env=$OPTARG @@ -29,7 +32,7 @@ while getopts ":e:u:r:h" opt; do u) export TF_VAR_user=$OPTARG ;; - r) + d) export TF_VAR_region=$OPTARG ;; h) @@ -62,7 +65,7 @@ if [[ -z $TF_VAR_user ]]; then fi if [[ -z $TF_VAR_region ]]; then - echo 'Error: must specify region by either providing the -r argument or setting TF_VAR_region.' + echo 'Error: must specify region by either providing the -d argument or setting TF_VAR_region.' exit 1 fi diff --git a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh index c53d368bf..efc292163 100644 --- a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh +++ b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh @@ -15,30 +15,34 @@ cd /home/ubuntu || exit # Install our environment variables -cat <<"EOF" > environment +cat <<"EOF" >environment ${foreman_environment} EOF # These database values are created after TF # is run, so we have to pass them in programatically -cat >> /home/ubuntu/run_foreman.sh <>/home/ubuntu/run_foreman.sh <> /home/ubuntu/run_management_command.sh + --detach \\ + --env DATABASE_HOST=${database_host} \\ + --env DATABASE_NAME=${database_name} \\ + --env DATABASE_PASSWORD=${database_password} \\ + --env DATABASE_USER=${database_user} \\ + --env-file /home/ubuntu/environment \\ + --interactive \\ + --tty \\ + --volume /tmp:/tmp \\ + ${dockerhub_repo}/${foreman_docker_image} \\ + python3 manage.py \"\$@\" +" >>/home/ubuntu/run_management_command.sh chmod +x /home/ubuntu/run_management_command.sh echo " @@ -70,21 +78,24 @@ echo " # the first argument followed by the management command to run. docker run \\ - --env-file /home/ubuntu/environment \\ - -e DATABASE_HOST=${database_host} \\ - -e DATABASE_NAME=${database_name} \\ - -e DATABASE_USER=${database_user} \\ - -e DATABASE_PASSWORD=${database_password} \\ - -v /tmp:/tmp \\ - -it ${dockerhub_repo}/dr_\"\$1\" python3 manage.py \"\$2\" -" >> /home/ubuntu/run_manage_command.sh + --env DATABASE_HOST=${database_host} \\ + --env DATABASE_NAME=${database_name} \\ + --env DATABASE_PASSWORD=${database_password} \\ + --env DATABASE_USER=${database_user} \\ + --env-file /home/ubuntu/environment \\ + --interactive \\ + --tty \\ + --volume /tmp:/tmp \\ + ${dockerhub_repo}/dr_\"\$1\" \\ + python3 manage.py \"\$2\" +" >>/home/ubuntu/run_manage_command.sh chmod +x /home/ubuntu/run_manage_command.sh # Use Monit to ensure the Foreman is always running apt-get -y update apt-get -y install monit htop -date +%s > /tmp/foreman_last_time +date +%s >/tmp/foreman_last_time chown ubuntu:ubuntu /tmp/foreman_last_time # shellcheck disable=2016 echo ' @@ -96,7 +107,7 @@ if (( $difftime > 1800 )); then exit 1; fi exit 0; -' >> /home/ubuntu/foreman_status.sh +' >>/home/ubuntu/foreman_status.sh chmod +x /home/ubuntu/foreman_status.sh echo ' @@ -105,13 +116,13 @@ check program foreman with path "/bin/bash /home/ubuntu/foreman_status.sh" as ui if status != 0 then restart set daemon 900 -' >> /etc/monit/monitrc +' >>/etc/monit/monitrc service monit restart # Install the cron job tests -crontab -l > tempcron -cat <> tempcron +crontab -l >tempcron +cat <>tempcron 0 12 * * MON /bin/bash /home/ubuntu/run_manage_command.sh affymetrix check_brainarray_gene_agreement >> /var/log/affymetrix_checks.log 2>&1 0 12 * * MON /bin/bash /home/ubuntu/run_manage_command.sh affymetrix check_tx_index_transcript_agreement >> /var/log/affymetrix_checks.log 2>&1 0 12 * * ${accession_gathering_job_run_day} /bin/bash /home/ubuntu/run_manage_command.sh foreman gather_weekly_accessions >> /var/log/weekly_accessions.log 2>&1 @@ -122,18 +133,22 @@ rm tempcron # Make sure every downloader job has a processor job! docker run \ - --env-file /home/ubuntu/environment \ - -e DATABASE_HOST="${database_host}" \ - -e DATABASE_NAME="${database_name}" \ - -e DATABASE_USER="${database_user}" \ - -e DATABASE_PASSWORD="${database_password}" \ - -v /tmp:/tmp \ - --log-driver=awslogs \ - --log-opt awslogs-region="${region}" \ - --log-opt awslogs-group="${log_group}" \ - --log-opt awslogs-stream="log-stream-foreman-${user}-${stage}" \ - --name=job_filler \ - -it -d "${dockerhub_repo}/${foreman_docker_image}" python3 manage.py create_missing_processor_jobs + --detach \ + --env DATABASE_HOST="${database_host}" \ + --env DATABASE_NAME="${database_name}" \ + --env DATABASE_PASSWORD="${database_password}" \ + --env DATABASE_USER="${database_user}" \ + --env-file /home/ubuntu/environment \ + --interactive \ + --log-driver=awslogs \ + --log-opt awslogs-group="${log_group}" \ + --log-opt awslogs-region="${region}" \ + --log-opt awslogs-stream="log-stream-foreman-${user}-${stage}" \ + --name=job_filler \ + --tty \ + --volume /tmp:/tmp \ + "${dockerhub_repo}/${foreman_docker_image}" \ + python3 manage.py create_missing_processor_jobs # Delete the cloudinit and syslog in production. export STAGE=${stage} diff --git a/scripts/common.sh b/scripts/common.sh index 612de1a7b..b30416abc 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -2,45 +2,40 @@ # These are lists of docker images that we use. The actual names end # up being /dr_ but this is useful for scripting. -export ALL_CCDL_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders foreman api" +export ALL_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders foreman api" # Sometimes we only want to work with the worker images. -export CCDL_WORKER_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders" +export WORKER_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders" -get_docker_db_ip_address () { - docker inspect -f '{{range .NetworkSettings.Networks}}{{.IPAddress}}{{end}}' drdb 2> /dev/null -} - -get_docker_es_ip_address () { - docker inspect -f '{{range .NetworkSettings.Networks}}{{.IPAddress}}{{end}}' dres 2> /dev/null -} - -# `coverage report -m` will always have an exit code of 0 which makes -# it seem like the test is passing. Therefore we store the exit code -# of running the tests as $exit_code, then report the coverage, and -# then exit with the appropriate code. -# This is done a function so arguments to the tests can be passed through. -run_tests_with_coverage () { - COVERAGE="coverage run --source=\".\" manage.py test --settings=tests.settings --no-input $*; exit_code=\$?;" - SAVE_REPORT="coverage xml -o data_store/coverage.xml;" - PRINT_REPORT="coverage report -m;" - RETURN="exit \$exit_code" - - echo "$COVERAGE $PRINT_REPORT $SAVE_REPORT $RETURN" -} +# Default Docker registry. +if [ -z "$DOCKERHUB_REPO" ]; then + export DOCKERHUB_REPO="ccdlstaging" +fi # This function checks whether a given docker image name ($1:$2) # exists in Docker Hub or not using Docker Hub API V2. Based on: # https://stackoverflow.com/questions/32113330/check-if-imagetag-combination-already-exists-on-docker-hub -docker_img_exists() { +docker_image_exists() { TOKEN=$(curl -s -H "Content-Type: application/json" -X POST \ - -d '{"username": "'"${DOCKER_ID}"'", "password": "'"${DOCKER_PASSWD}"'"}' \ - https://hub.docker.com/v2/users/login/ | jq -r .token) + -d '{"username": "'"${DOCKER_ID}"'", "password": "'"${DOCKER_PASSWD}"'"}' \ + https://hub.docker.com/v2/users/login/ | jq -r .token) EXISTS=$(curl -s -H "Authorization: JWT ${TOKEN}" \ - "https://hub.docker.com/v2/repositories/$1/tags/?page_size=10000" \ - | jq -r "[.results | .[] | .name == \"$2\"] | any" 2> /dev/null) + "https://hub.docker.com/v2/repositories/$1/tags/?page_size=10000" | + jq -r "[.results | .[] | .name == \"$2\"] | any" 2>/dev/null) test -n "$EXISTS" -a "$EXISTS" = true } +get_branch_hash() { + git rev-parse --abbrev-ref HEAD | shasum | awk '{print $1}' +} + +get_docker_db_ip_address() { + docker inspect -f '{{range .NetworkSettings.Networks}}{{.IPAddress}}{{end}}' drdb 2>/dev/null +} + +get_docker_es_ip_address() { + docker inspect -f '{{range .NetworkSettings.Networks}}{{.IPAddress}}{{end}}' dres 2>/dev/null +} + # A tag is linked to a commit hash, not a branch. A single commit hash # can end up on multiple branches. So we first check to see if we're # on master, then on dev, then error out because we should only deploy master or dev. @@ -57,10 +52,69 @@ get_master_or_dev() { # All dev versions should end with '-dev' or '-dev-hotfix' and all master versions should not. if [ -n "$master_check" ] && ! echo "$version" | grep -Eq "\-dev(\-hotfix)?$"; then echo "master" - elif [ -n "$dev_check" ] ; then + elif [ -n "$dev_check" ]; then echo "dev" else echo "unknown" fi fi } + +# `coverage report -m` will always have an exit code of 0 which makes +# it seem like the test is passing. Therefore we store the exit code +# of running the tests as $exit_code, then report the coverage, and +# then exit with the appropriate code. +# This is done a function so arguments to the tests can be passed through. +run_tests_with_coverage() { + COVERAGE="coverage run --source=\".\" manage.py test --settings=tests.settings --no-input $*; exit_code=\$?;" + SAVE_REPORT="coverage xml -o data_store/coverage.xml;" + PRINT_REPORT="coverage report -m;" + RETURN="exit \$exit_code" + + echo "$COVERAGE $PRINT_REPORT $SAVE_REPORT $RETURN" +} + +# Create docker-container/desktop-linux Docker builder if none provided. +# Set the builder as currently used. +set_up_docker_builder() { + if [ -z "$DOCKER_BUILDER" ]; then + echo "Setting up refine.bio Docker builder." + + if test "$GITHUB_ACTION"; then + echo "$INSTANCE_SSH_KEY" >infrastructure/data-refinery-key.pem + chmod 600 infrastructure/data-refinery-key.pem + + # shellcheck disable=SC2046 + eval $(ssh-agent) + ssh-add infrastructure/data-refinery-key.pem + + if [ ! -d ~/.ssh ]; then + mkdir -m 700 ~/.ssh + fi + cat >~/.ssh/config </dev/null || + true + else + DOCKER_BUILDER="refinebio_local_builder" + echo "Creating Docker builder $DOCKER_BUILDER." + docker buildx create \ + --driver=docker-container \ + --name="$DOCKER_BUILDER" \ + --platform=linux/amd64 2>/dev/null || + true + fi + fi + + echo "Using Docker builder $DOCKER_BUILDER." + docker buildx use "$DOCKER_BUILDER" +} diff --git a/scripts/format_batch_with_env.sh b/scripts/format_batch_with_env.sh index a86ee36b5..21938e1e5 100755 --- a/scripts/format_batch_with_env.sh +++ b/scripts/format_batch_with_env.sh @@ -24,34 +24,34 @@ print_options() { while getopts ":p:e:o:v:h" opt; do case $opt in - p) - export project="$OPTARG" - ;; - e) - export env="$OPTARG" - ;; - o) - export output_dir="$OPTARG" - ;; - v) - export system_version="$OPTARG" - ;; - h) - print_description - echo - print_options - exit 0 - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - print_options >&2 - exit 1 - ;; - :) - echo "Option -$OPTARG requires an argument." >&2 - print_options >&2 - exit 1 - ;; + p) + export project="$OPTARG" + ;; + e) + export env="$OPTARG" + ;; + o) + export output_dir="$OPTARG" + ;; + v) + export system_version="$OPTARG" + ;; + h) + print_description + echo + print_options + exit 0 + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + print_options >&2 + exit 1 + ;; + :) + echo "Option -$OPTARG requires an argument." >&2 + print_options >&2 + exit 1 + ;; esac done @@ -102,10 +102,12 @@ if [ -z "$COMPENDIA_DOCKER_IMAGE" ]; then export COMPENDIA_DOCKER_IMAGE="dr_compendia:$system_version" fi - # This script should always run from the context of the directory of # the project it is building. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" project_directory="$script_directory/.." @@ -142,7 +144,7 @@ while read -r line; do # shellcheck disable=SC2163 export "$line" fi -done < "$environment_file" +done <"$environment_file" # If output_dir wasn't specified then assume the same folder we're # getting the templates from. @@ -154,7 +156,6 @@ if [ ! -d "$output_dir" ]; then mkdir "$output_dir" fi - # Not quite sure how to deal with this just yet, so punt. # export INDEX=0 @@ -163,7 +164,7 @@ fi if [ "$project" = "workers" ]; then # Iterate over all the template files in the directory. for template in batch-job-templates/*.tpl.json; do - template="$(basename "$template")" + template="$(basename "$template")" # Strip off the trailing .tpl for once we've formatted it. OUTPUT_BASE="$(basename "$template" .tpl.json)" FILETYPE=".json" @@ -173,66 +174,62 @@ if [ "$project" = "workers" ]; then if [ "$OUTPUT_FILE" = "downloader.json" ]; then rams="1024 4096 16384" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/downloader$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done echo "Made $output_dir/$OUTPUT_FILE" elif [ "$OUTPUT_FILE" = "create_compendia.json" ]; then rams="30000 950000" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/$OUTPUT_BASE$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done echo "Made $output_dir/$OUTPUT_FILE" elif [ "$OUTPUT_FILE" = "create_quantpendia.json" ]; then rams="30000 131000" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/$OUTPUT_BASE$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done echo "Made $output_dir/$OUTPUT_FILE" # From https://unix.stackexchange.com/a/111517 elif (echo "$NO_RAM_JOB_FILES" | grep -Fqw "$OUTPUT_FILE"); then perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$output_dir/$OUTPUT_FILE" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$output_dir/$OUTPUT_FILE" \ + 2>/dev/null echo "Made $output_dir/$OUTPUT_FILE" else rams="2048 4096 8192 12288 16384 32768 65536" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/$OUTPUT_BASE$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done fi @@ -241,7 +238,7 @@ elif [ "$project" = "surveyor" ]; then # Iterate over all the template files in the directory. for template in batch-job-templates/*.tpl.json; do - template="$(basename "$template")" + template="$(basename "$template")" # Strip off the trailing .tpl for once we've formatted it. OUTPUT_BASE="$(basename "$template" .tpl.json)" FILETYPE=".json" @@ -249,21 +246,20 @@ elif [ "$project" = "surveyor" ]; then if [ "$OUTPUT_FILE" = "surveyor_dispatcher.json" ]; then perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$output_dir/$OUTPUT_FILE" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$output_dir/$OUTPUT_FILE" \ + 2>/dev/null echo "Made $output_dir/$OUTPUT_FILE" else rams="1024 4096 16384" - for r in $rams - do + for r in $rams; do export RAM_POSTFIX="_$r" export RAM="$r" FILEPATH="$output_dir/$OUTPUT_BASE$RAM_POSTFIX$FILETYPE" perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < "batch-job-templates/$template" \ - > "$FILEPATH" \ - 2> /dev/null + <"batch-job-templates/$template" \ + >"$FILEPATH" \ + 2>/dev/null echo "Made $FILEPATH" done fi @@ -272,12 +268,12 @@ elif [ "$project" = "surveyor" ]; then elif [ "$project" = "foreman" ]; then # foreman sub-project perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < environment.tpl \ - > "$output_dir/environment" \ - 2> /dev/null + "$output_dir/environment" \ + 2>/dev/null elif [ "$project" = "api" ]; then perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ - < environment.tpl \ - > "$output_dir/environment" \ - 2> /dev/null + "$output_dir/environment" \ + 2>/dev/null fi diff --git a/scripts/install_all.sh b/scripts/install_all.sh index 0d389712e..30a34c080 100755 --- a/scripts/install_all.sh +++ b/scripts/install_all.sh @@ -1,6 +1,6 @@ #!/bin/sh -# Exit on error +# Exit on error. set -e # Config variables @@ -8,7 +8,10 @@ TERRAFORM_VERSION="0.13.5" # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit print_description() { @@ -37,117 +40,117 @@ confirm() { printf "%s [y/N] " "$1" read -r confirmation if ! [ "$confirmation" = "y" ]; then - echo "Confirmation failure" >&2 + echo "Confirmation failure" >&2 exit 1 fi } while getopts "hv" opt; do case $opt in - h) - print_description - echo - print_usage - exit 0 - ;; - v) - OUTPUT="/dev/stdout" - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - print_usage >&2 - exit 1 - ;; + h) + print_description + echo + print_usage + exit 0 + ;; + v) + OUTPUT="/dev/stdout" + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + print_usage >&2 + exit 1 + ;; esac done # Unless output was set to stdout by the verbose flag, set it to /dev/null -# to hide the stdout of package management commands +# to hide the stdout of package management commands. if [ -z "$OUTPUT" ]; then OUTPUT="/dev/null" fi if [ -z "$INSTALL_CMD" ]; then case "$(uname)" in - "Darwin") - if ! command -v brew >/dev/null; then - confirm "Would you like to install Homebrew?" - /usr/bin/ruby -e "$(curl -fsSL https://raw.githubusercontent.com/Homebrew/install/master/install)" - fi - - INSTALL_CMD="brew install" - INSTALL_CASK_CMD="brew cask install" - BREW=true - ;; - "Linux") - if command -v apt >/dev/null; then - sudo apt-get update - INSTALL_CMD="sudo apt-get install --assume-yes" - APT=true - else - echo "Your Linux distribution is not officially supported," >&2 - echo "but it *should* be able to run the required services. You need to manually" >&2 - echo "install dependencies or give the command to install dependencies with \$INSTALL_CMD." >&2 - exit 1 - fi - ;; - *) - echo "$(uname) is an unsupported operating system." >&2 - echo "You can try to provide a package manager command with \$INSTALL_CMD," >&2 - echo "but your mileage may vary." >&2 + "Darwin") + if ! command -v brew >/dev/null; then + confirm "Would you like to install Homebrew?" + /usr/bin/ruby -e "$(curl -fsSL https://raw.githubusercontent.com/Homebrew/install/master/install)" + fi + + INSTALL_CMD="brew install" + INSTALL_CASK_CMD="brew cask install" + BREW=true + ;; + "Linux") + if command -v apt >/dev/null; then + sudo apt-get update + INSTALL_CMD="sudo apt-get install --assume-yes" + APT=true + else + echo "Your Linux distribution is not officially supported," >&2 + echo "but it *should* be able to run the required services. You need to manually" >&2 + echo "install dependencies or give the command to install dependencies with \$INSTALL_CMD." >&2 exit 1 - ;; + fi + ;; + *) + echo "$(uname) is an unsupported operating system." >&2 + echo "You can try to provide a package manager command with \$INSTALL_CMD," >&2 + echo "but your mileage may vary." >&2 + exit 1 + ;; esac fi -if ! command -v docker > /dev/null; then +if ! command -v docker >/dev/null; then echo "Installing Docker..." - # On macOS, install docker desktop with Homebrew cask - if [ $BREW ]; then - $INSTALL_CASK_CMD docker > $OUTPUT + # On macOS, install docker desktop with Homebrew cask. + if [ "$BREW" ]; then + $INSTALL_CASK_CMD docker >"$OUTPUT" else - $INSTALL_CMD docker.io > $OUTPUT || (echo "You must manually install docker" && exit 1) + $INSTALL_CMD docker.io >"$OUTPUT" || (echo "You must manually install Docker" && exit 1) - echo "Fixing docker permissions..." + echo "Fixing Docker permissions..." sudo groupadd -f docker sudo usermod -aG docker "$USER" - echo - echo "Logout and log back in to apply the permissions changes, then execute this script again." - exit 0 + echo + echo "Logout and log back in to apply the permissions changes, then execute this script again." + exit 0 fi fi -if ! command -v pip3 > /dev/null && ! [ $BREW ]; then # Don't reinstall python on macOS - echo "Installing python and pip..." - $INSTALL_CMD python3-pip > $OUTPUT || (echo "You must manually install python and pip" && exit 1) +if ! command -v pip3 >/dev/null && ! [ "$BREW" ]; then # Don't reinstall python on macOS + echo "Installing Python and pip..." + $INSTALL_CMD python3-pip >"$OUTPUT" || (echo "You must manually install Python and pip" && exit 1) fi -if ! command -v terraform > /dev/null; then - echo "Installing terraform..." - if [ $BREW ]; then - $INSTALL_CMD terraform > $OUTPUT - elif [ $APT ] || confirm "Would you like to automatically install Terraform for amd64 linux?"; then - $INSTALL_CMD unzip > $OUTPUT +if ! command -v terraform >/dev/null; then + echo "Installing Terraform..." + if [ "$BREW" ]; then + $INSTALL_CMD terraform >"$OUTPUT" + elif [ "$APT" ] || confirm "Would you like to automatically install Terraform for amd64 Linux?"; then + $INSTALL_CMD unzip >"$OUTPUT" curl -0s "https://releases.hashicorp.com/terraform/${TERRAFORM_VERSION}/terraform_${TERRAFORM_VERSION}_linux_amd64.zip" \ - > "terraform_${TERRAFORM_VERSION}_linux_amd64.zip" + >"terraform_${TERRAFORM_VERSION}_linux_amd64.zip" sudo unzip -d /usr/bin "terraform_${TERRAFORM_VERSION}_linux_amd64.zip" sudo chmod a+rx /usr/bin/terraform - rm "terraform_${TERRAFORM_VERSION}_linux_amd64.zip" + rm "terraform_${TERRAFORM_VERSION}_linux_amd64.zip" else echo "You need to manually install Terraform before continuing..." >&2 exit 1 fi fi -if ! command -v pre-commit > /dev/null; then +if ! command -v pre-commit >/dev/null; then message="Would you like to automatically install pre-commit? \ Note: This will install all the required dependencies (black, isort, etc) \ using an additional ~185MB of disk space." - if [ $APT ] || confirm "$message"; then + if [ "$APT" ] || confirm "$message"; then echo "Installing pre-commit..." - $INSTALL_CMD shellcheck > $OUTPUT + $INSTALL_CMD shellcheck >"$OUTPUT" pip3 install pre-commit pre-commit install else @@ -155,32 +158,32 @@ using an additional ~185MB of disk space." fi fi -if ! command -v jq > /dev/null; then +if ! command -v jq >/dev/null; then echo "Installing jq..." - $INSTALL_CMD jq > $OUTPUT || (echo "You must manually install jq" && exit 1) + $INSTALL_CMD jq >"$OUTPUT" || (echo "You must manually install jq" && exit 1) fi -if ! command -v ip > /dev/null; then - if [ $BREW ]; then - $INSTALL_CMD iproute2mac > $OUTPUT +if ! command -v ip >/dev/null; then + if [ "$BREW" ]; then + $INSTALL_CMD iproute2mac >"$OUTPUT" else - $INSTALL_CMD iproute2 > $OUTPUT || (echo "You must manually install iproute2" && exit 1) + $INSTALL_CMD iproute2 >"$OUTPUT" || (echo "You must manually install iproute2" && exit 1) fi fi echo "Starting postgres and installing the database..." -./run_postgres.sh > $OUTPUT -./install_db_docker.sh > $OUTPUT +./run_postgres.sh >"$OUTPUT" +./install_db_docker.sh >"$OUTPUT" echo "Starting elasticsearch and building the ES Indexes..." -./run_es.sh > $OUTPUT -./rebuild_es_index.sh > $OUTPUT +./run_es.sh >"$OUTPUT" +./rebuild_es_index.sh >"$OUTPUT" echo "Creating virtual environment..." -./create_virtualenv.sh > $OUTPUT +./create_virtualenv.sh >"$OUTPUT" echo "Run \`source dr_env/bin/activate\` to activate the virtual environment." echo "Updating common dependencies..." -# Source the virtual environment first +# Source the virtual environment first. . ../dr_env/bin/activate -./update_models.sh > $OUTPUT +./update_models.sh >"$OUTPUT" diff --git a/scripts/install_db_docker.sh b/scripts/install_db_docker.sh index 52d2e3138..8dafc2354 100755 --- a/scripts/install_db_docker.sh +++ b/scripts/install_db_docker.sh @@ -1,8 +1,45 @@ #! /bin/sh -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c "create database data_refinery" -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c "CREATE ROLE data_refinery_user WITH LOGIN PASSWORD 'data_refinery_password';" -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c 'GRANT ALL PRIVILEGES ON DATABASE data_refinery TO data_refinery_user;' -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c 'ALTER USER data_refinery_user CREATEDB;' -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c 'ALTER ROLE data_refinery_user superuser;' -h postgres -U postgres -docker run -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -c 'CREATE EXTENSION IF NOT EXISTS hstore;' -h postgres -U postgres -d data_refinery +POSTGRES_VERSION="9.6.6" + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c "create database data_refinery" -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c "CREATE ROLE data_refinery_user WITH LOGIN PASSWORD 'data_refinery_password';" -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c 'GRANT ALL PRIVILEGES ON DATABASE data_refinery TO data_refinery_user;' -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c 'ALTER USER data_refinery_user CREATEDB;' -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c 'ALTER ROLE data_refinery_user superuser;' -h postgres -U postgres + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --link drdb:postgres \ + --rm \ + "postgres:$POSTGRES_VERSION" \ + psql -c 'CREATE EXTENSION IF NOT EXISTS hstore;' -h postgres -U postgres -d data_refinery diff --git a/scripts/kill_all_jobs.sh b/scripts/kill_all_jobs.sh index 6ef70be94..4da0eafa4 100755 --- a/scripts/kill_all_jobs.sh +++ b/scripts/kill_all_jobs.sh @@ -2,13 +2,16 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit while read -r row; do # Exporting an expansion rather than a variable, which is exactly what we want to do. # shellcheck disable=SC2163 export "${row}" -done < ../infrastructure/prod_env +done <../infrastructure/prod_env python3 kill_all_jobs.py diff --git a/scripts/make_migrations.sh b/scripts/make_migrations.sh index 25ef9e6c8..807531569 100755 --- a/scripts/make_migrations.sh +++ b/scripts/make_migrations.sh @@ -3,34 +3,44 @@ # Script for migrating the database using a Docker container so no # virtual environment is needed on the host machine. -# Exit on error +# Exit on error. set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit ./prepare_image.sh -i migrations -s common . ./common.sh + DB_HOST_IP=$(get_docker_db_ip_address) docker run \ - --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ - --add-host=database:"$DB_HOST_IP" \ - --env-file ../common/environments/local \ - --interactive \ - ccdlstaging/dr_migrations python3 manage.py makemigrations data_refinery_common + --add-host=database:"$DB_HOST_IP" \ + --env-file ../common/environments/local \ + --interactive \ + --platform linux/amd64 \ + --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ + "$DOCKERHUB_REPO/dr_migrations" \ + python3 manage.py makemigrations data_refinery_common docker run \ - --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ - --add-host=database:"$DB_HOST_IP" \ - --env-file ../common/environments/local \ - ccdlstaging/dr_migrations python3 manage.py migrate + --add-host=database:"$DB_HOST_IP" \ + --env-file ../common/environments/local \ + --platform linux/amd64 \ + --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ + "$DOCKERHUB_REPO/dr_migrations" \ + python3 manage.py migrate docker run \ - --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ - --add-host=database:"$DB_HOST_IP" \ - --env-file ../common/environments/local \ - ccdlstaging/dr_migrations python3 manage.py createcachetable + --add-host=database:"$DB_HOST_IP" \ + --env-file ../common/environments/local \ + --platform linux/amd64 \ + --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ + "$DOCKERHUB_REPO/dr_migrations" \ + python3 manage.py createcachetable diff --git a/scripts/prepare_image.sh b/scripts/prepare_image.sh index 81975da90..4bdff97c4 100755 --- a/scripts/prepare_image.sh +++ b/scripts/prepare_image.sh @@ -2,10 +2,12 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# Import the functions in common.sh . ./common.sh # We need access to all of the projects @@ -18,108 +20,129 @@ print_description() { print_options() { echo "Options:" - echo " -h Prints the help message" + echo " -h Prints the help message." echo " -i IMAGE The image to be prepared. This must be specified." echo " -s SERVICE The service to seach for a dockerfile." - echo " The default option is 'workers'" - echo " -p Pull the latest version of the image from Dockerhub" - echo " -d REPO The docker repo to pull images from." - echo " The default option is 'ccdl'" + echo " The default option is 'workers'." + echo " -u Push the built image to the Dockerhub." + echo " -r REPO The docker registry to use for pull/push actions." + echo " The default option is 'ccdlstaging'." echo echo "Examples:" echo " Build the image ccdl/dr_downloaders:" - echo " ./scripts/prepare_image.sh -i downloaders -d ccdl" + echo " ./scripts/prepare_image.sh -i downloaders -r ccdlstaging" } -while getopts "phi:d:s:" opt; do +while getopts "uhi:r:s:" opt; do case $opt in - i) - image=$OPTARG - ;; - d) - dockerhub_repo=$OPTARG - ;; - p) - pull="True" - ;; - s) - service=$OPTARG - ;; - h) - print_description - echo - print_options - exit 0 - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - print_options >&2 - exit 1 - ;; - :) - echo "Option -$OPTARG requires an argument." >&2 - print_options >&2 - exit 1 - ;; + i) + IMAGE_NAME="$OPTARG" + ;; + r) + DOCKERHUB_REPO="$OPTARG" + ;; + + s) + SERVICE="$OPTARG" + ;; + u) + DOCKER_ACTION="--push" + ;; + h) + print_description + echo + print_options + exit 0 + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + print_options >&2 + exit 1 + ;; + :) + echo "Option -$OPTARG requires an argument." >&2 + print_options >&2 + exit 1 + ;; esac done -if [ -z "$image" ]; then +if [ -z "$IMAGE_NAME" ]; then echo "Error: you must specify an image with -i" >&2 exit 1 fi - -if [ -z "$service" ]; then - service="workers" +if [ -z "$SERVICE" ]; then + SERVICE="workers" fi -if [ -z "$dockerhub_repo" ]; then - dockerhub_repo="ccdlstaging" +if [ -z "$DOCKERHUB_REPO" ]; then + DOCKERHUB_REPO="ccdlstaging" fi -# Default to "local" for system version if we're not running in the cloud. +# Defaults to commit hash value for if we're not running in the cloud. if [ -z "$SYSTEM_VERSION" ]; then - SYSTEM_VERSION="local$(date +%s)" + SYSTEM_VERSION="$(get_branch_hash)" fi -# We want to check if a test image has been built for this branch. If -# it has we should use that rather than building it slowly. -image_name="$dockerhub_repo/dr_$image" -# shellcheck disable=SC2086 -if [ "$(docker_img_exists $image_name $branch_name)" ] ; then - docker pull "$image_name:$branch_name" -elif [ -n "$pull" ]; then - docker pull "$image_name" -else - echo "" - echo "Rebuilding the $image_name image." - finished=1 - attempts=0 - while [ $finished != 0 ] && [ $attempts -lt 3 ]; do - if [ $attempts -gt 0 ]; then - echo "Failed to build $image_name, trying again." - fi - - - if test "$GITHUB_ACTIONS"; then - # docker needs repositories to be lowercase - CACHE_REPO="$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]')" - CACHED_PACKAGE="$CACHE_REPO/dr_$image" - CACHE="--build-arg BUILDKIT_INLINE_CACHE=1 --cache-from $CACHED_PACKAGE" - fi - - docker build \ - -t "$image_name" \ - -f "$service/dockerfiles/Dockerfile.$image" \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ - $CACHE . - finished=$? - attempts=$((attempts+1)) - done - - if [ $finished != 0 ] && [ $attempts -ge 3 ]; then - echo "Could not build $image_name after three attempts." - exit 1 +if [ -z "$DOCKER_ACTION" ]; then + DOCKER_ACTION="--load" +fi + +DOCKERHUB_IMAGE="$DOCKERHUB_REPO/dr_$IMAGE_NAME" + +CACHE_FROM_LATEST="cache-from=type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest" +CACHE_FROM_VERSION="cache-from=type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION" +CACHE_TO_LATEST="cache-to=type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" +CACHE_TO_VERSION="cache-to=type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" + +if test "$GITHUB_ACTION"; then + CACHE_TO_LATEST="cache-to=type=gha" + CACHE_TO_VERSION="cache-to=type=gha" + DOCKER_ACTION="--push" +fi + +DOCKER_FILE_PATH="$SERVICE/dockerfiles/Dockerfile.$IMAGE_NAME" + +echo +echo "Building the $IMAGE_NAME:$SYSTEM_VERSION image from $DOCKER_FILE_PATH." +echo + +attempt=0 +attempts=3 +finished=1 +while [ $finished != 0 ] && [ $attempt -lt $attempts ]; do + if [ $attempt -gt 0 ]; then + echo "Failed to build $IMAGE_NAME:$SYSTEM_VERSION image, trying again." fi + + set_up_docker_builder + + docker buildx build \ + --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ + --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ + --"$CACHE_FROM_LATEST" \ + --"$CACHE_FROM_VERSION" \ + --"$CACHE_TO_LATEST" \ + --"$CACHE_TO_VERSION" \ + --file "$DOCKER_FILE_PATH" \ + --platform linux/amd64 \ + --tag "$DOCKERHUB_IMAGE:latest" \ + --tag "$DOCKERHUB_IMAGE:$SYSTEM_VERSION" \ + "$DOCKER_ACTION" \ + . + + finished=$? + attempt=$((attempt + 1)) +done + +if [ $finished -ne 0 ] && [ $attempt -ge $attempts ]; then + echo "Could not build $DOCKERHUB_IMAGE after $attempt attempts." + exit 1 +fi + +if test "$GITHUB_ACTION"; then + docker pull \ + --platform linux/amd64 \ + "$DOCKERHUB_IMAGE" fi diff --git a/scripts/rebuild_es_index.sh b/scripts/rebuild_es_index.sh index 30fded027..c3ac7745b 100755 --- a/scripts/rebuild_es_index.sh +++ b/scripts/rebuild_es_index.sh @@ -2,7 +2,10 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit ./run_manage.sh -i api_local -s api search_index --rebuild -f diff --git a/scripts/reinit_database.sh b/scripts/reinit_database.sh index 18107000d..d397c9c14 100755 --- a/scripts/reinit_database.sh +++ b/scripts/reinit_database.sh @@ -2,10 +2,12 @@ # Reintializes the database so there's no data or migrations run against it. - # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Clear it out. diff --git a/scripts/run_all_tests.sh b/scripts/run_all_tests.sh index 9372e43f2..20f7b4a14 100755 --- a/scripts/run_all_tests.sh +++ b/scripts/run_all_tests.sh @@ -7,7 +7,10 @@ set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Get access to all of the refinebio project diff --git a/scripts/run_es.sh b/scripts/run_es.sh index c300aca2f..9c2a9681a 100755 --- a/scripts/run_es.sh +++ b/scripts/run_es.sh @@ -1,13 +1,21 @@ #! /bin/sh -docker rm -f dres 2> /dev/null +docker rm -f dres 2>/dev/null -# Check if a docker database named "dres" exists, and if so just start it +# Check if a docker database named "dres" exists, and if so just start it. if [ "$(docker ps -a --filter name=dres -q)" ]; then - docker start dres > /dev/null -# Otherwise, run it with `docker run` + docker start dres >/dev/null +# Otherwise, run it with `docker run`. else - docker run --name dres -p 9200:9200 -p 9300:9300 -e "discovery.type=single-node" -e "indices.query.bool.max_clause_count=16384" -d docker.elastic.co/elasticsearch/elasticsearch:6.5.4 + docker run \ + --detach \ + --env "discovery.type=single-node" \ + --env "indices.query.bool.max_clause_count=16384" \ + --name dres \ + --platform linux/amd64 \ + --publish 9200:9200 \ + --publish 9300:9300 \ + docker.elastic.co/elasticsearch/elasticsearch:6.5.4 fi echo "Started ElasticSearch." diff --git a/scripts/run_manage.sh b/scripts/run_manage.sh index 51cc2f550..899d05c29 100755 --- a/scripts/run_manage.sh +++ b/scripts/run_manage.sh @@ -6,13 +6,16 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# Import the functions in common.sh +# Import the functions in common.sh. . ./common.sh -# We need access to all of the projects +# We need access to all of the projects. cd .. print_description() { @@ -44,7 +47,7 @@ if [ "$1" = "-i" ]; then shift if [ -z "$1" ]; then echo "Error: Missing argument for -i" >&2 - echo + echo print_options >&2 exit 1 fi @@ -62,7 +65,7 @@ if [ "$1" = "-s" ]; then shift if [ -z "$1" ]; then echo "Error: Missing argument for -s" >&2 - echo + echo print_options >&2 exit 1 fi @@ -72,29 +75,44 @@ else service="foreman" fi -# Set up the data volume directory if it does not already exist +if [ -z "$SYSTEM_VERSION" ]; then + SYSTEM_VERSION="$(get_branch_hash)" +fi + +# Set up the data volume directory if it does not already exist. volume_directory="$script_directory/../api/volume" if [ ! -d "$volume_directory" ]; then mkdir "$volume_directory" fi chmod -R a+rwX "$volume_directory" -docker build -t dr_shell -f "$service/dockerfiles/Dockerfile.$image" . +docker build \ + --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ + --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ + --file "$service/dockerfiles/Dockerfile.$image" \ + --platform linux/amd64 \ + --tag dr_shell \ + . DB_HOST_IP=$(get_docker_db_ip_address) ES_HOST_IP=$(get_docker_es_ip_address) -# Only run interactively if we are on a TTY +# Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi -docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --add-host=elasticsearch:"$ES_HOST_IP" \ - --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ - --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ - --env-file "$service/environments/local" \ - --volume /tmp:/tmp \ - --volume "$volume_directory":/home/user/data_store \ - dr_shell python3 manage.py "$@" +# shellcheck disable=SC2086 +docker run \ + --add-host=database:"$DB_HOST_IP" \ + --add-host=elasticsearch:"$ES_HOST_IP" \ + --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ + --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ + --env-file "$service/environments/local" \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + --volume /tmp:/tmp \ + $INTERACTIVE \ + dr_shell \ + python3 manage.py "$@" diff --git a/scripts/run_postgres.sh b/scripts/run_postgres.sh index 2798dc04b..6ad077bce 100755 --- a/scripts/run_postgres.sh +++ b/scripts/run_postgres.sh @@ -2,30 +2,39 @@ # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Get access to all of refinebio cd .. -# CircleCI Docker won't make this by default for some reason +# CircleCI Docker won't make this by default for some reason. # This doubly nested directory is a hacky workaround to prevent permissions issues. # Suggested here: # https://github.com/docker/for-linux/issues/380#issuecomment-436419102 VOLUMES="$script_directory/../volumes_postgres/volumes_postgres" if [ ! -d "$VOLUMES" ]; then - mkdir -p "$VOLUMES" + mkdir -p "$VOLUMES" fi # Check if a docker database named "drdb" exists, and if so just run it if [ "$(docker ps -a --filter name=drdb -q)" ]; then - docker start drdb > /dev/null - echo "Started database." -# Otherwise, install it from docker hub + docker start drdb >/dev/null + echo "Started database." +# Otherwise, install it from Docker Hub. else - # via https://hub.docker.com/_/postgres/ - # 9.6.6 is the current (as of Jan 23 2018) RDS most recent version. - # Password can be exposed to git/CI because this is only for dev/testing purposes, not real data. - echo "Installing database..." - docker run -p 5432:5432 --name drdb -v "$VOLUMES":/var/lib/postgresql/data -e POSTGRES_PASSWORD=mysecretpassword -d postgres:9.6.6 + # via https://hub.docker.com/_/postgres/ + # 9.6.6 is the current (as of Jan 23 2018) RDS most recent version. + # Password can be exposed to git/CI because this is only for dev/testing purposes, not real data. + echo "Installing database..." + docker run \ + --detach \ + --env POSTGRES_PASSWORD=mysecretpassword \ + --name drdb \ + --publish 5432:5432 \ + --volume "$VOLUMES":/var/lib/postgresql/data \ + postgres:9.6.6 fi diff --git a/scripts/run_psql_shell.sh b/scripts/run_psql_shell.sh index 9d99de96a..98939c0a0 100755 --- a/scripts/run_psql_shell.sh +++ b/scripts/run_psql_shell.sh @@ -1,2 +1,10 @@ #! /bin/sh -docker run -it -e PGPASSWORD=mysecretpassword --rm --link drdb:postgres postgres:9.6.6 psql -h postgres -U postgres -d data_refinery + +docker run \ + --env PGPASSWORD=mysecretpassword \ + --interactive \ + --link drdb:postgres \ + --rm \ + --tty \ + postgres:9.6.6 \ + psql -h postgres -U postgres -d data_refinery diff --git a/scripts/run_shell.sh b/scripts/run_shell.sh index cfb60d00c..7760d9f2e 100755 --- a/scripts/run_shell.sh +++ b/scripts/run_shell.sh @@ -12,7 +12,10 @@ set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # Import functions in common.sh @@ -28,15 +31,21 @@ if [ ! -d "$volume_directory" ]; then fi chmod -R a+rwX "$volume_directory" -docker build -t dr_shell -f foreman/dockerfiles/Dockerfile.foreman . +docker build \ + --file foreman/dockerfiles/Dockerfile.foreman \ + --tag dr_shell \ + . DB_HOST_IP=$(get_docker_db_ip_address) -docker run -it \ - --add-host="database:$DB_HOST_IP" \ - --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ - --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ - --env-file foreman/environments/local \ - --volume /tmp:/tmp \ - --volume "$volume_directory":/home/user/data_store \ - --interactive dr_shell python3 manage.py shell +docker run \ + --add-host="database:$DB_HOST_IP" \ + --env AWS_ACCESS_KEY_ID="$AWS_ACCESS_KEY_ID" \ + --env AWS_SECRET_ACCESS_KEY="$AWS_SECRET_ACCESS_KEY" \ + --env-file foreman/environments/local \ + --interactive \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + --volume /tmp:/tmp \ + dr_shell \ + python3 manage.py shell diff --git a/scripts/update_docker_images.sh b/scripts/update_docker_images.sh new file mode 100755 index 000000000..f051e8317 --- /dev/null +++ b/scripts/update_docker_images.sh @@ -0,0 +1,136 @@ +#!/bin/sh + +# Exit on failure. +set -e + +# This script should always run as if it were being called from +# the directory it lives in. +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" +cd "$script_directory" || exit + +. ./common.sh + +# Get access to all of refinebio. +cd .. + +print_description() { + echo 'This script will re-build all refine.bio docker images and push ' + echo 'them to the specified Dockerhub repository.' +} + +print_options() { + cat <&2 + print_options >&2 + exit 1 + ;; + :) + echo "Option -$OPTARG requires an argument." >&2 + print_options >&2 + exit 1 + ;; + esac +done + +if [ -z "$DOCKERHUB_REPO" ]; then + echo 'Error: must specify the Dockerhub repository with -r' + exit 1 +fi + +if [ -z "$SYSTEM_VERSION" ]; then + SYSTEM_VERSION="$(get_branch_hash)" +fi + +# Intentionally omit affymetrix unless specifically requested since it is so +# intense to build. +image_names="base migrations common_tests foreman api_base api_production api_local \ + transcriptome smasher salmon no_op illumina downloaders compendia" +if [ "$BUILD_AFFYMETRIX" ]; then + image_names="$image_names affymetrix" +fi + +# Set the version for the common project. +echo "$SYSTEM_VERSION" >common/version + +# Create common/dist/data-refinery-common-*.tar.gz, which is +# required by the workers and data_refinery_foreman images. +# Remove old common distributions if they exist. +rm -f common/dist/* +(cd common && python3 setup.py sdist 1>/dev/null) # Run quietly in a subshell. + +# shellcheck disable=SC2086 +for image_name in $image_names; do + case $image_name in + api_base | api_local | api_production) + DOCKER_FILE_PATH="api/dockerfiles/Dockerfile.$image_name" + ;; + base | common_tests | migrations) + DOCKER_FILE_PATH="common/dockerfiles/Dockerfile.$image_name" + ;; + foreman) + DOCKER_FILE_PATH="foreman/dockerfiles/Dockerfile.$image_name" + ;; + *) + DOCKER_FILE_PATH="workers/dockerfiles/Dockerfile.$image_name" + ;; + esac + + echo + echo "Building the $image_name:$SYSTEM_VERSION image from $DOCKER_FILE_PATH." + echo + + DOCKERHUB_IMAGE="$DOCKERHUB_REPO/dr_$image_name" + CACHE_FROM_LATEST="cache-from=type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest" + CACHE_FROM_VERSION="cache-from=type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION" + CACHE_TO_LATEST="cache-to=type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" + CACHE_TO_VERSION="cache-to=type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" + + set_up_docker_builder + + docker buildx build \ + --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ + --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ + --"$CACHE_FROM_LATEST" \ + --"$CACHE_FROM_VERSION" \ + --"$CACHE_TO_LATEST" \ + --"$CACHE_TO_VERSION" \ + --file "$DOCKER_FILE_PATH" \ + --platform linux/amd64 \ + --push \ + --tag "$DOCKERHUB_IMAGE:latest" \ + --tag "$DOCKERHUB_IMAGE:$SYSTEM_VERSION" \ + . +done diff --git a/scripts/update_models.sh b/scripts/update_models.sh index d6648df8f..d04fd6810 100755 --- a/scripts/update_models.sh +++ b/scripts/update_models.sh @@ -1,31 +1,36 @@ #! /bin/sh -# Makes migrations and re-installs so Docker images update locally +# Makes migrations and re-installs so Docker images update locally. -# Exit on fail +# Exit on fail. set -e # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# Get access to all of refinebio +# Get access to all of refinebio. cd .. -if ! docker ps | tail -n +2 | awk '{ print $NF }' | grep drdb > /dev/null; then +if ! docker ps | tail -n +2 | awk '{ print $NF }' | grep drdb >/dev/null; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 exit 1 fi -# Default to "0.0.0.dev" for system version if we're not running in the cloud. +. ./scripts/common.sh + +# Default to the branch name hash for system version if we're not running in the cloud. if [ -z "$SYSTEM_VERSION" ]; then - SYSTEM_VERSION="0.0.0.dev$(date +%s)" + SYSTEM_VERSION="$(get_branch_hash)" export SYSTEM_VERSION fi # Put this in place for common to read from. -echo "$SYSTEM_VERSION" > common/version +echo "$SYSTEM_VERSION" >common/version # Ensure there is only one distribution to copy over. rm -f common/dist/* diff --git a/scripts/update_my_docker_images.sh b/scripts/update_my_docker_images.sh deleted file mode 100755 index e49d1d542..000000000 --- a/scripts/update_my_docker_images.sh +++ /dev/null @@ -1,126 +0,0 @@ -#!/bin/sh - -# This script is very similar to .circleci/update_docker_images.sh but it has less -# production/cloud related checks. - -# Exit on failure -set -e - -# This script should always run as if it were being called from -# the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" -cd "$script_directory" || exit - -# Get access to all of refinebio -cd .. - -print_description() { - echo 'This script will re-build all refine.bio docker images and push them to' - echo 'the specified Dockerhub repo.' -} - -print_options() { - cat << EOF -There are two required arguments for this script: --d specifies the Dockerhub repo you would like to deploy to. --v specifies the version you would like to build. This version will passed into - the Docker image as the environment variable SYSTEM_VERSION. - It also will be used as the tag for the Docker images built. - -There is also one optional argument: --a also build the affymetrix image - (we normally don't because it is so intense to build) -EOF -} - -while getopts ":d:v:ah" opt; do - case $opt in - d) - export DOCKERHUB_REPO="$OPTARG" - ;; - v) - export SYSTEM_VERSION="$OPTARG" - ;; - a) - AFFYMETRIX=true - ;; - h) - print_description - echo - print_options - exit 0 - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - print_options >&2 - exit 1 - ;; - :) - echo "Option -$OPTARG requires an argument." >&2 - print_options >&2 - exit 1 - ;; - esac -done - -if [ -z "$DOCKERHUB_REPO" ]; then - echo 'Error: must specify the Dockerhub repo with -d' - exit 1 -fi - -if [ -z "$SYSTEM_VERSION" ]; then - echo 'Error: must specify the version repo with -v' - exit 1 -fi - -# Intentionally omit affymetrix unless specifically requested since it is so intense to build. -CCDL_WORKER_IMGS="salmon transcriptome no_op downloaders illumina smasher compendia" -if [ "$AFFYMETRIX" ]; then - CCDL_WORKER_IMGS="$CCDL_WORKER_IMGS affymetrix" -fi - -# Set the version for the common project. -echo "$SYSTEM_VERSION" > common/version - -# Create common/dist/data-refinery-common-*.tar.gz, which is -# required by the workers and data_refinery_foreman images. -## Remove old common distributions if they exist -rm -f common/dist/* -(cd common && python3 setup.py sdist) - -for IMG in $CCDL_WORKER_IMGS; do - image_name="$DOCKERHUB_REPO/dr_$IMG" - - echo "Building docker image: $image_name:$SYSTEM_VERSION" - # Build and push image. - docker build \ - -t "$image_name:$SYSTEM_VERSION" \ - -f "workers/dockerfiles/Dockerfile.$IMG" \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" . - docker push "$image_name:$SYSTEM_VERSION" - # Update latest version - docker tag "$image_name:$SYSTEM_VERSION" "$image_name:latest" - docker push "$image_name:latest" -done - -# Build and push Foreman image. -FOREMAN_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_foreman" -docker build \ - -t "$FOREMAN_DOCKER_IMAGE:$SYSTEM_VERSION" \ - -f foreman/dockerfiles/Dockerfile.foreman \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" . -docker push "$FOREMAN_DOCKER_IMAGE:$SYSTEM_VERSION" -# Update latest version -docker tag "$FOREMAN_DOCKER_IMAGE:$SYSTEM_VERSION" "$FOREMAN_DOCKER_IMAGE:latest" -docker push "$FOREMAN_DOCKER_IMAGE:latest" - -# Build and push API image. -API_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_api" -docker build \ - -t "$API_DOCKER_IMAGE:$SYSTEM_VERSION" \ - -f api/dockerfiles/Dockerfile.api_production \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" . -docker push "$API_DOCKER_IMAGE:$SYSTEM_VERSION" -# Update latest version -docker tag "$API_DOCKER_IMAGE:$SYSTEM_VERSION" "$API_DOCKER_IMAGE:latest" -docker push "$API_DOCKER_IMAGE:latest" diff --git a/workers/R/renv_load.R b/workers/R/renv_load.R deleted file mode 100644 index dd5caa080..000000000 --- a/workers/R/renv_load.R +++ /dev/null @@ -1,16 +0,0 @@ -options(warn = 2) -options(repos = structure(c( - Bioconductor = "https://bioconductor.org/packages/3.6/bioc", - BioconductorAnnotation = "https://bioconductor.org/packages/3.6/data/annotation", - BioconductorExperiment = "https://bioconductor.org/packages/3.6/data/experiment", - CRAN = "https://cloud.r-project.org" -))) -options(Ncpus = parallel::detectCores()) -options(renv.r.version = "3.4.4") -options(renv.settings.use.cache = FALSE) - -install.packages("BiocInstaller") -install.packages("https://cran.r-project.org/src/contrib/Archive/renv/renv_0.16.0.tar.gz") - -renv::consent(provided = TRUE) -renv::restore(prompt = FALSE, rebuild = TRUE) diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index 6ac42f4ef..8dad322b3 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -1,4 +1,6 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] @@ -6,7 +8,7 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/affymetrix/renv.lock . -COPY workers/R/renv_load.R . +COPY common/R/renv_load.R . RUN Rscript renv_load.R COPY workers/R/dependencies/affymetrix/install_ensg_pkgs.R . @@ -26,7 +28,6 @@ COPY workers/ . RUN rm -rf /root/.cache/* -ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.affymetrix_local b/workers/dockerfiles/Dockerfile.affymetrix_local deleted file mode 100644 index 7fe881ab0..000000000 --- a/workers/dockerfiles/Dockerfile.affymetrix_local +++ /dev/null @@ -1,29 +0,0 @@ -FROM ccdlstaging/dr_affymetrix:latest - -# Fail in case of an error at any stage in the pipe. -SHELL ["/bin/bash", "-o", "pipefail", "-c"] - -USER root - -WORKDIR /home/user - -# Remove the version of common already installed. -RUN rm -rf common && \ - pip3 uninstall -y data_refinery_common - -# Get the latest version from the dist directory. -COPY common/dist/data-refinery-common-* common/ -RUN pip3 install --ignore-installed --no-cache-dir \ - common/$(ls common -1 | sort --version-sort | tail -1) - -COPY config/ config/ -COPY workers/ . - -RUN rm -rf /root/.cache/* - -ARG SYSTEM_VERSION -ENV SYSTEM_VERSION=$SYSTEM_VERSION - -USER user - -ENTRYPOINT [] diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 7b8240b78..371abf2c7 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -68,7 +68,7 @@ WORKDIR /home/user ENV R_LIBS=/usr/local/lib/R/site-library COPY workers/R/dependencies/compendia/renv.lock . -COPY workers/R/renv_load.R . +COPY common/R/renv_load.R . RUN Rscript renv_load.R COPY workers/data_refinery_workers/requirements/compendia.txt requirements.txt @@ -82,7 +82,8 @@ RUN wget -q "https://bitbucket.org/ariya/phantomjs/downloads/\ phantomjs-2.1.1-linux-x86_64.tar.bz2" && \ tar xvjf phantomjs-2.1.1-linux-x86_64.tar.bz2 -C /usr/local/share/ && \ ln -s /usr/local/share/phantomjs-2.1.1-linux-x86_64/bin/phantomjs \ - /usr/local/bin/ + /usr/local/bin/ && \ + rm phantomjs-2.1.1-linux-x86_64.tar.bz2 # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 1b19485e2..5361db49f 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -1,4 +1,6 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] @@ -6,7 +8,7 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/downloaders/renv.lock . -COPY workers/R/renv_load.R . +COPY common/R/renv_load.R . RUN Rscript renv_load.R COPY workers/data_refinery_workers/downloaders/requirements.txt . @@ -34,7 +36,6 @@ COPY workers/ . RUN rm -rf /root/.cache/* -ARG SYSTEM_VERSION ENV PATH="$PATH:/home/user/.aspera/ascli/sdk" ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index b89630096..1d771b322 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -1,4 +1,6 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] @@ -6,7 +8,7 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/illumina/renv.lock . -COPY workers/R/renv_load.R . +COPY common/R/renv_load.R . RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . @@ -25,7 +27,6 @@ COPY workers/illumina_probe_maps/ probe_maps/ RUN rm -rf /root/.cache/* -ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index 11729dba4..7e33a14e3 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -1,4 +1,6 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] @@ -6,7 +8,7 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/no_op/renv.lock . -COPY workers/R/renv_load.R . +COPY common/R/renv_load.R . RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . @@ -35,7 +37,6 @@ COPY workers/ . RUN rm -rf /root/.cache/* -ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index d9cd8818e..08a3d3299 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -1,4 +1,6 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] @@ -6,7 +8,7 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/salmon/renv.lock . -COPY workers/R/renv_load.R . +COPY common/R/renv_load.R . RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . @@ -29,7 +31,7 @@ v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ -C "Salmon-${SALMON_VERSION}_linux_x86_64" --strip-components 1 && \ ln -sf "$(pwd)/Salmon-${SALMON_VERSION}_linux_x86_64/bin/salmon" \ /usr/local/bin/ && \ - rm -f "Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" + rm "Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" # End Salmon installation. ENV SRA_VERSION=2.9.1 @@ -45,7 +47,8 @@ RUN git clone https://github.com/COMBINE-lab/SalmonTools.git && \ wget -q "https://ftp.ncbi.nlm.nih.gov/sra/sdk/${SRA_VERSION}/\ sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ tar zxfv "sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" && \ - cp -r "sratoolkit.${SRA_VERSION}-ubuntu64/bin/"* /usr/bin + cp -r "sratoolkit.${SRA_VERSION}-ubuntu64/bin/"* /usr/bin && \ + rm "sratoolkit.${SRA_VERSION}-ubuntu64.tar.gz" # Get the latest version from the dist directory. COPY common/dist/data-refinery-common-* common/ @@ -58,7 +61,6 @@ COPY workers/ . RUN rm -rf /root/.cache/* -ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index 29bba5cd8..f95da5016 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -1,4 +1,6 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] @@ -6,7 +8,7 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/smasher/renv.lock . -COPY workers/R/renv_load.R . +COPY common/R/renv_load.R . RUN Rscript renv_load.R COPY workers/data_refinery_workers/processors/requirements.txt . @@ -23,7 +25,6 @@ COPY workers/ . RUN rm -rf /root/.cache/* -ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index 3aec1395b..e3c5328a4 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -1,17 +1,12 @@ -FROM ccdlstaging/dr_base:latest +ARG DOCKERHUB_REPO +ARG SYSTEM_VERSION +FROM $DOCKERHUB_REPO/dr_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user -COPY workers/data_refinery_workers/processors/requirements.txt . -RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ - # It's annoying that this can only be installed via git. - git clone https://github.com/deweylab/RSEM.git && \ - cd RSEM && make install && \ - rm -rf RSEM - # Install Salmon. # Tximport requires all experiments to be processed with the same version of # Salmon to work https://github.com/AlexsLemonade/refinebio/issues/1496. @@ -19,6 +14,7 @@ RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ # all samples from incomplete experiments must have salmon run on them again. ENV SALMON_VERSION=0.13.1 +COPY workers/data_refinery_workers/processors/requirements.txt . # Salmon can extract to a different directory than the name of the tar file. RUN wget -q "https://github.com/COMBINE-lab/salmon/releases/download/\ v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ @@ -30,6 +26,13 @@ v${SALMON_VERSION}/Salmon-${SALMON_VERSION}_linux_x86_64.tar.gz" && \ rm -r Salmon* # End Salmon installation. +COPY workers/data_refinery_workers/processors/requirements.txt . +RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt && \ + # It's annoying that this can only be installed via git. + git clone https://github.com/deweylab/RSEM.git && \ + cd RSEM && make install && \ + rm -rf RSEM + COPY common/dist/data-refinery-common-* common/ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) @@ -40,7 +43,6 @@ COPY workers/ . RUN rm -rf /root/.cache/* -ARG SYSTEM_VERSION ENV SYSTEM_VERSION=$SYSTEM_VERSION USER user diff --git a/workers/run_command.sh b/workers/run_command.sh index da50660b1..51dc3b6c9 100755 --- a/workers/run_command.sh +++ b/workers/run_command.sh @@ -7,18 +7,18 @@ set -e while getopts "i:" opt; do case $opt in - i) - image=$OPTARG - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - exit 1 - ;; + i) + IMAGE="$OPTARG" + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + exit 1 + ;; esac done -if [ -z "$image" ]; then - image="smasher" +if [ -z "$IMAGE" ]; then + IMAGE="smasher" else shift shift @@ -26,7 +26,10 @@ fi # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to @@ -47,16 +50,19 @@ fi chmod -R a+rwX "$volume_directory" . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) -./scripts/prepare_image.sh -i "$image" -s workers -image_name=ccdlstaging/dr_"$image" +./scripts/prepare_image.sh -i "$IMAGE" -s workers docker run \ - --add-host=database:"$DB_HOST_IP" \ - --env-file workers/environments/local \ - --env AWS_ACCESS_KEY_ID \ - --env AWS_SECRET_ACCESS_KEY \ - --volume "$volume_directory":/home/user/data_store \ - --link drdb:postgres \ - -it "$image_name" bash -c "$@" + --add-host=database:"$DB_HOST_IP" \ + --env AWS_ACCESS_KEY_ID \ + --env AWS_SECRET_ACCESS_KEY \ + --env-file workers/environments/local \ + --ineractive \ + --link drdb:postgres \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + "$DOCKERHUB_REPO/dr_$IMAGE" \ + bash -c "$@" diff --git a/workers/run_janitor.sh b/workers/run_janitor.sh index 27c6ed6f5..70d9dcbde 100755 --- a/workers/run_janitor.sh +++ b/workers/run_janitor.sh @@ -1,19 +1,20 @@ #!/bin/sh -# the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +# The directory it lives in. +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to -# move up a level +# However, in order to give Docker access to all the code we have to +# move up a level. cd .. ./scripts/prepare_image.sh -i smasher -image_name="ccdlstaging/dr_smasher" - -volume_directory="$script_directory/volume" . ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) AWS_ACCESS_KEY_ID="$(~/bin/aws configure get default.aws_access_key_id)" @@ -22,13 +23,15 @@ AWS_SECRET_ACCESS_KEY="$(~/bin/aws configure get default.aws_secret_access_key)" export AWS_SECRET_ACCESS_KEY docker run \ - -it \ - -m 500m \ - --add-host=database:"$DB_HOST_IP" \ - --env-file workers/environments/local \ - --env AWS_ACCESS_KEY_ID \ - --env AWS_SECRET_ACCESS_KEY \ - --entrypoint ./manage.py \ - --volume "$volume_directory":/home/user/data_store \ - --link drdb:postgres \ - "$image_name" run_janitor + --add-host=database:"$DB_HOST_IP" \ + --entrypoint ./manage.py \ + --env AWS_ACCESS_KEY_ID \ + --env AWS_SECRET_ACCESS_KEY \ + --env-file workers/environments/local \ + --interactive \ + --link drdb:postgres \ + --memory 500m \ + --tty \ + --volume "$script_directory/volume":/home/user/data_store \ + "$DOCKERHUB_REPO/dr_smasher" \ + run_janitor diff --git a/workers/run_job.sh b/workers/run_job.sh index 7342103bc..47d95f192 100755 --- a/workers/run_job.sh +++ b/workers/run_job.sh @@ -1,67 +1,70 @@ #!/bin/bash -# Script for running a django management command to test the worker. +# Script for running a Django management command to test the worker. while getopts "hi:" opt; do case $opt in - i) - image=$OPTARG - ;; - h) - echo "Runs a downloader or processor job. The following arguments are supported:" - echo "-h : Print this help message and exit." - echo "-i : The image to use. Options are:" - echo " downloaders (default)" - echo " salmon" - echo " transcriptome" - echo " no_op" - echo " downloaders" - echo " illumina" - echo " affymetrix" - echo " : What kind of job to run." - echo " Must be either 'run_downloader_job' or 'run_processor_job'." - echo "--job-name= : The type of job to run." - echo " For processor jobs, options are:" - echo " AFFY_TO_PCL" - echo " AGILENT_TWOCOLOR_TO_PCL" - echo " SALMON" - echo " ILLUMINA_TO_PCL" - echo " TRANSCRIPTOME_INDEX_LONG" - echo " TRANSCRIPTOME_INDEX_SHORT" - echo " NO_OP" - echo " For downloader jobs, options are:" - echo " ARRAY_EXPRESS" - echo " SRA" - echo " TRANSCRIPTOME_INDEX" - echo " GEO" - echo "--job-id= : The id of the job you want to run. Must already exist in the database." - echo "" - echo "Note that the must correspond to the ." - echo " AGILENT_TWOCOLOR_TO_PCL is a special case because it requires the 'affymetrix' image." - echo "" - echo "Examples:" - echo " ./workers/run_job.sh run_downloader_job --job-name=SRA --job-id=12345" - echo " ./workers/run_job.sh -i affymetrix run_processor_job --job-name=AGILENT_TWOCOLOR_TO_PCL --job-id=54321" - exit 0 - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - exit 1 - ;; - :) - echo "Option -$OPTARG requires an argument." >&2 - exit 1 - ;; + i) + IMAGE="$OPTARG" + ;; + h) + echo "Runs a downloader or processor job. The following arguments are supported:" + echo "-h : Print this help message and exit." + echo "-i : The image to use. Options are:" + echo " downloaders (default)" + echo " salmon" + echo " transcriptome" + echo " no_op" + echo " downloaders" + echo " illumina" + echo " affymetrix" + echo " : What kind of job to run." + echo " Must be either 'run_downloader_job' or 'run_processor_job'." + echo "--job-name= : The type of job to run." + echo " For processor jobs, options are:" + echo " AFFY_TO_PCL" + echo " AGILENT_TWOCOLOR_TO_PCL" + echo " SALMON" + echo " ILLUMINA_TO_PCL" + echo " TRANSCRIPTOME_INDEX_LONG" + echo " TRANSCRIPTOME_INDEX_SHORT" + echo " NO_OP" + echo " For downloader jobs, options are:" + echo " ARRAY_EXPRESS" + echo " SRA" + echo " TRANSCRIPTOME_INDEX" + echo " GEO" + echo "--job-id= : The id of the job you want to run. Must already exist in the database." + echo "" + echo "Note that the must correspond to the ." + echo " AGILENT_TWOCOLOR_TO_PCL is a special case because it requires the 'affymetrix' image." + echo "" + echo "Examples:" + echo " ./workers/run_job.sh run_downloader_job --job-name=SRA --job-id=12345" + echo " ./workers/run_job.sh -i affymetrix run_processor_job --job-name=AGILENT_TWOCOLOR_TO_PCL --job-id=54321" + exit 0 + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + exit 1 + ;; + :) + echo "Option -$OPTARG requires an argument." >&2 + exit 1 + ;; esac done -if [[ -z "$image" ]]; then - image="downloaders" +if [[ -z "$IMAGE" ]]; then + IMAGE="downloaders" fi # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit # However in order to give Docker access to all the code we have to @@ -69,12 +72,12 @@ cd "$script_directory" || exit cd .. # Agilent uses the same image as affymetrix -if [[ "$image" == "affymetrix" || "$image" == "agilent" ]]; then - ./scripts/prepare_image.sh -p -i affymetrix - image_name="ccdlstaging/dr_affymetrix" +if [[ "$IMAGE" == "affymetrix" || "$IMAGE" == "agilent" ]]; then + ./scripts/prepare_image.sh -i affymetrix + IMAGE_NAME="$DOCKERHUB_REPO/dr_affymetrix" else - ./scripts/prepare_image.sh -i "$image" - image_name="ccdlstaging/dr_$image" + ./scripts/prepare_image.sh -i "$IMAGE" + IMAGE_NAME="$DOCKERHUB_REPO/dr_$IMAGE" fi volume_directory="$script_directory/volume" @@ -83,16 +86,20 @@ if [ ! -d "$volume_directory" ]; then fi chmod -R a+rwX "$volume_directory" -source scripts/common.sh +. scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) docker run \ - -it \ - --add-host=database:"$DB_HOST_IP" \ - --env-file workers/environments/local \ - --env AWS_ACCESS_KEY_ID \ - --env AWS_SECRET_ACCESS_KEY \ - --entrypoint ./manage.py \ - --volume "$volume_directory":/home/user/data_store \ - --link drdb:postgres \ - "$image_name" "${@: -3}" "${@: -2}" "${@: -1}" + --add-host=database:"$DB_HOST_IP" \ + --entrypoint ./manage.py \ + --env AWS_ACCESS_KEY_ID \ + --env AWS_SECRET_ACCESS_KEY \ + --env-file workers/environments/local \ + --interactive \ + --link drdb:postgres \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + "$IMAGE_NAME" \ + "${@: -3}" "${@: -2}" "${@: -1}" diff --git a/workers/run_tests.sh b/workers/run_tests.sh index 3e03e2ada..a66d0a2f7 100755 --- a/workers/run_tests.sh +++ b/workers/run_tests.sh @@ -3,7 +3,7 @@ # Script for executing Django PyUnit tests within a Docker container. -# Exit on failure +# Exit on failure. set -e print_description() { @@ -20,47 +20,49 @@ print_options() { while getopts ":t:h" opt; do case $opt in - t) - tag=$OPTARG - ;; - h) - print_description - echo - print_options - exit 0 - ;; - \?) - echo "Invalid option: -$OPTARG" >&2 - print_options >&2 - exit 1 - ;; - :) - echo "Option -$OPTARG requires an argument." >&2 - print_options >&2 - exit 1 - ;; + t) + tag="$OPTARG" + ;; + h) + print_description + echo + print_options + exit 0 + ;; + \?) + echo "Invalid option: -$OPTARG" >&2 + print_options >&2 + exit 1 + ;; + :) + echo "Option -$OPTARG requires an argument." >&2 + print_options >&2 + exit 1 + ;; esac done # This script should always run as if it were being called from # the directory it lives in. -script_directory="$(cd "$(dirname "$0")" || exit; pwd)" +script_directory="$( + cd "$(dirname "$0")" || exit + pwd +)" cd "$script_directory" || exit -# However in order to give Docker access to all the code we have to +# However, in order to give Docker access to all the code we have to # move up a level cd .. -# Ensure that postgres is running +# Ensure that Postgres is running. if ! [ "$(docker ps --filter name=drdb -q)" ]; then echo "You must start Postgres first with:" >&2 echo "./scripts/run_postgres.sh" >&2 exit 1 fi -volume_directory="$script_directory/test_volume" - test_data_repo="https://s3.amazonaws.com/data-refinery-test-assets" +volume_directory="$script_directory/test_volume" if [ -z "$tag" ] || [ "$tag" = "salmon" ]; then # Download "salmon quant" test data The `newer` file was to @@ -77,11 +79,11 @@ if [ -z "$tag" ] || [ "$tag" = "salmon" ]; then rm "$volume_directory"/salmon_tests.tar.gz fi - # salmontools test data + # SalmonTools test data. salmontools_test_zip="$test_data_repo/salmontools_test_data.tar.gz" salmontools_test_dir="$volume_directory/salmontools" - # Clean the test data directory + # Clean the test data directory. rm -rf "$salmontools_test_dir" mkdir -p "$salmontools_test_dir" @@ -102,21 +104,21 @@ if [ -z "$tag" ] || [ "$tag" = "salmon" ]; then mkdir -p "$rna_seq_test_raw_dir" echo "Downloading $read_1_name for Salmon tests." wget -q -O "$rna_seq_test_data_1" \ - "$test_data_repo/$read_1_name" + "$test_data_repo/$read_1_name" echo "Downloading $read_2_name for Salmon tests." wget -q -O "$rna_seq_test_data_2" \ - "$test_data_repo/$read_2_name" + "$test_data_repo/$read_2_name" fi if [ ! -e "$dotsra" ]; then mkdir -p "$rna_seq_test_raw_dir" echo "Downloading $dotsra_name for Salmon tests." wget -q -O "$dotsra" \ - "$test_data_repo/$dotsra_name" + "$test_data_repo/$dotsra_name" fi fi if [ -z "$tag" ] || [ "$tag" = "affymetrix" ]; then - # Make sure CEL for test is downloaded from S3 + # Make sure CEL for test is downloaded from S3. cel_name="GSM1426071_CD_colon_active_1.CEL" cel_name2="GSM45588.CEL" cel_name3="GSM1364667_U_110208_7-02-10_S2.CEL" @@ -135,35 +137,35 @@ if [ -z "$tag" ] || [ "$tag" = "affymetrix" ]; then mkdir -p "$cel_test_raw_dir" echo "Downloading CEL for tests." wget -q -O "$cel_test_data_1" \ - "$test_data_repo/$cel_name" + "$test_data_repo/$cel_name" fi if [ ! -e "$cel_test_data_2" ]; then echo "Downloading Non-Brainarray CEL for tests." wget -q -O "$cel_test_data_2" \ - "$test_data_repo/$cel_name2" + "$test_data_repo/$cel_name2" fi if [ ! -e "$cel_test_data_3" ]; then echo "Downloading Huex Brain Array CEL for tests." wget -q -O "$cel_test_data_3" \ - "$test_data_repo/$cel_name3" + "$test_data_repo/$cel_name3" fi if [ ! -e "$pcl_test_data_1" ]; then mkdir -p "$pcl_test_dir" echo "Downloading pre-computed PCL for tests." wget -q -O "$pcl_test_data_1" \ - "$test_data_repo/$pcl_name" + "$test_data_repo/$pcl_name" fi if [ ! -e "$pcl_test_data_2" ]; then mkdir -p "$pcl_test_dir" echo "Downloading pre-computed Non-Brainarray PCL for tests." wget -q -O "$pcl_test_data_2" \ - "$test_data_repo/$pcl_name2" + "$test_data_repo/$pcl_name2" fi if [ ! -e "$pcl_test_data_3" ]; then mkdir -p "$pcl_test_dir" echo "Downloading pre-computed Huex Brain Array PCL for tests." wget -q -O "$pcl_test_data_3" \ - "$test_data_repo/$pcl_name3" + "$test_data_repo/$pcl_name3" fi fi @@ -176,14 +178,14 @@ if [ -z "$tag" ] || [ "$tag" = "transcriptome" ]; then mkdir -p "$tx_index_test_raw_dir" echo "Downloading fasta file for Transcriptome Index tests." wget -q -O "$tx_index_test_raw_dir/$fasta_file" \ - "$test_data_repo/$fasta_file" + "$test_data_repo/$fasta_file" fi gtf_file="aegilops_tauschii_short.gtf.gz" if [ ! -e "$tx_index_test_raw_dir/$gtf_file" ]; then mkdir -p "$tx_index_test_raw_dir" echo "Downloading gtf file for Transcriptome Index tests." wget -q -O "$tx_index_test_raw_dir/$gtf_file" \ - "$test_data_repo/$gtf_file" + "$test_data_repo/$gtf_file" fi tx_index_test_raw_dir2="$volume_directory/raw/TEST/TRANSCRIPTOME_INDEX/" gtf_file2="Homo_sapiens_testdata.gtf" @@ -191,7 +193,7 @@ if [ -z "$tag" ] || [ "$tag" = "transcriptome" ]; then mkdir -p "$tx_index_test_raw_dir2" echo "Downloading second gtf file for Transcriptome Index tests." wget -q -O "$tx_index_test_raw_dir2/$gtf_file2" \ - "$test_data_repo/$gtf_file2" + "$test_data_repo/$gtf_file2" fi fi @@ -210,18 +212,17 @@ GSE41355_non-normalized.txt GSE100301_non-normalized.txt' "$test_data_repo/$ilu_file" fi - i=$(( i + 1 )) + i=$((i + 1)) done unset i - ilu_test_ref_dir="$volume_directory/raw/TEST/ILLUMINA/reference" ilu_ref_file="Ad-Cre-2.AVG_Signal.tsv" if [ ! -e "$ilu_test_ref_dir/$ilu_ref_file" ]; then mkdir -p "$ilu_test_ref_dir" echo "Downloading Illumin reference file for Illumina tests." wget -q -O "$ilu_test_ref_dir/$ilu_ref_file" \ - "$test_data_repo/$ilu_ref_file" + "$test_data_repo/$ilu_ref_file" fi fi @@ -233,7 +234,7 @@ if [ -z "$tag" ] || [ "$tag" = "agilent" ]; then mkdir -p "$at_test_raw_dir" echo "Downloading Agilent file for A2C tests." wget -q -O "$at_test_raw_dir/$at_file" \ - "$test_data_repo/$at_file" + "$test_data_repo/$at_file" fi fi if [ -z "$tag" ] || [ "$tag" = "no_op" ]; then @@ -243,37 +244,37 @@ if [ -z "$tag" ] || [ "$tag" = "no_op" ]; then mkdir -p "$no_test_raw_dir" echo "Downloading NOOP file1." wget -q -O "$no_test_raw_dir/$no_file1" \ - "$test_data_repo/$no_file1" + "$test_data_repo/$no_file1" fi no_file2="GSM1234847_sample_table.txt" if [ ! -e "$no_test_raw_dir/$no_file2" ]; then mkdir -p "$no_test_raw_dir" echo "Downloading NOOP file2." wget -q -O "$no_test_raw_dir/$no_file2" \ - "$test_data_repo/$no_file2" + "$test_data_repo/$no_file2" fi no_file3="GSM1234847_sample_table_headerless.txt" if [ ! -e "$no_test_raw_dir/$no_file3" ]; then mkdir -p "$no_test_raw_dir" echo "Processing NOOP file3." - tail -n +2 "$no_test_raw_dir/$no_file2" > "$no_test_raw_dir/$no_file3" + tail -n +2 "$no_test_raw_dir/$no_file2" >"$no_test_raw_dir/$no_file3" fi no_file4="GSM1089291-tbl-1.txt" if [ ! -e "$no_test_raw_dir/$no_file4" ]; then mkdir -p "$no_test_raw_dir" echo "Downloading NOOP file4." wget -q -O "$no_test_raw_dir/$no_file4" \ - "$test_data_repo/$no_file4" + "$test_data_repo/$no_file4" fi no_file5="GSM1089291-tbl-1-modified.txt" if [ ! -e "$no_test_raw_dir/$no_file5" ]; then mkdir -p "$no_test_raw_dir" echo "Downloading NOOP file5." wget -q -O "$no_test_raw_dir/$no_file5" \ - "$test_data_repo/$no_file5" + "$test_data_repo/$no_file5" fi - # Reference files + # Reference files. no_test_exp_dir="$volume_directory/TEST/NO_OP/EXPECTED" no_test_exp_files='gene_converted_GSM557500-tbl-1.txt GSM269747.PCL gene_converted_GSM1234847-tbl-1.txt gene_converted_GSM1089291-tbl-1.txt' mkdir -p "$no_test_exp_dir" @@ -283,16 +284,16 @@ if [ -z "$tag" ] || [ "$tag" = "no_op" ]; then if ! [ -e "$no_test_exp_dir/$no_test_exp_file" ]; then echo "Downloading NOOP expected file$i." wget -O "$no_test_exp_dir/$no_test_exp_file" \ - "$test_data_repo/$no_test_exp_file" + "$test_data_repo/$no_test_exp_file" fi - i=$(( i + 1 )) + i=$((i + 1)) done unset i fi if [ -z "$tag" ] || [ "$tag" = "smasher" ] || [ "$tag" = "compendia" ]; then - # Make sure PCL for test is downloaded from S3 + # Make sure PCL for test is downloaded from S3. pcl_name="GSM1237810_T09-1084.PCL" pcl_name2="GSM1237812_S97-PURE.PCL" pcl_name3="GSM1238108-tbl-1.txt" @@ -334,101 +335,101 @@ if [ -z "$tag" ] || [ "$tag" = "smasher" ] || [ "$tag" = "compendia" ]; then mkdir -p "$pcl_test_raw_dir" echo "Downloading PCL for tests." wget -q -O "$pcl_test_data_1" \ - "$test_data_repo/$pcl_name" + "$test_data_repo/$pcl_name" fi if [ ! -e "$pcl_test_data_2" ]; then echo "Downloading PCL2 for tests." wget -q -O "$pcl_test_data_2" \ - "$test_data_repo/$pcl_name2" + "$test_data_repo/$pcl_name2" fi if [ ! -e "$pcl_test_data_3" ]; then echo "Downloading PCL3 for tests." wget -q -O "$pcl_test_data_3" \ - "$test_data_repo/$pcl_name3" + "$test_data_repo/$pcl_name3" fi if [ ! -e "$pcl_test_data_4" ]; then echo "Downloading PCL4 for tests." wget -q -O "$pcl_test_data_4" \ - "$test_data_repo/$pcl_name4" + "$test_data_repo/$pcl_name4" fi if [ ! -e "$pcl_test_data_5" ]; then echo "Downloading PCL5 for tests." wget -q -O "$pcl_test_data_5" \ - "$test_data_repo/$pcl_name5" + "$test_data_repo/$pcl_name5" fi if [ ! -e "$pcl_test_data_6" ]; then echo "Downloading PCL6 for tests." wget -q -O "$pcl_test_data_6" \ - "$test_data_repo/$pcl_name6" + "$test_data_repo/$pcl_name6" fi if [ ! -e "$pcl_test_data_7" ]; then echo "Downloading PCL7 for tests." wget -q -O "$pcl_test_data_7" \ - "$test_data_repo/$pcl_name7" + "$test_data_repo/$pcl_name7" fi if [ ! -e "$pcl_test_data_gs1" ]; then echo "Downloading PCLGS1 for tests." wget -q -O "$pcl_test_data_gs1" \ - "$test_data_repo/$pcl_name_gs1" + "$test_data_repo/$pcl_name_gs1" fi if [ ! -e "$pcl_test_data_gs2" ]; then echo "Downloading PCLGS2 for tests." wget -q -O "$pcl_test_data_gs2" \ - "$test_data_repo/$pcl_name_gs2" + "$test_data_repo/$pcl_name_gs2" fi if [ ! -e "$pcl_test_data_ts1" ]; then echo "Downloading PCLTS1 for tests." wget -q -O "$pcl_test_data_ts1" \ - "$test_data_repo/$pcl_name_ts1" + "$test_data_repo/$pcl_name_ts1" fi if [ ! -e "$pcl_test_data_ts2" ]; then echo "Downloading PCLTS2 for tests." wget -q -O "$pcl_test_data_ts2" \ - "$test_data_repo/$pcl_name_ts2" + "$test_data_repo/$pcl_name_ts2" fi if [ ! -e "$pcl_test_data_ta1" ]; then echo "Downloading PCLTA1 for tests." wget -q -O "$pcl_test_data_ta1" \ - "$test_data_repo/$pcl_name_ta1" + "$test_data_repo/$pcl_name_ta1" fi if [ ! -e "$bad_test_data_1" ]; then mkdir -p "$bad_test_raw_dir" echo "Downloading Bad PCL for tests." wget -q -O "$bad_test_data_1" \ - "$test_data_repo/$bad_name" + "$test_data_repo/$bad_name" fi if [ ! -e "$bad_test_data_2" ]; then mkdir -p "$bad_test_raw_dir" echo "Downloading Bad PCL for tests." wget -q -O "$bad_test_data_2" \ - "$test_data_repo/$bad_name2" + "$test_data_repo/$bad_name2" fi if [ ! -e "$bad_test_data_3" ]; then mkdir -p "$bad_test_raw_dir" echo "Downloading Bad PCL for tests." wget -q -O "$bad_test_data_3" \ - "$test_data_repo/$bad_name3" + "$test_data_repo/$bad_name3" fi if [ ! -e "$quant_test_data_1" ]; then mkdir -p "$quant_test_raw_dir" echo "Downloading Quant files for tests." wget -q -O "$quant_test_data_1" \ - "$test_data_repo/$quant_name" + "$test_data_repo/$quant_name" fi if [ ! -e "$quant_test_data_2" ]; then mkdir -p "$quant_test_raw_dir" echo "Downloading Quant files for tests." wget -q -O "$quant_test_data_2" \ - "$test_data_repo/$quant_name_2" + "$test_data_repo/$quant_name_2" fi # Mock out the AWS keys since we use VCR to mock out the request with these - # as the AWS credentials + # as the AWS credentials. export AWS_ACCESS_KEY_ID=XXX export AWS_SECRET_ACCESS_KEY=XXX fi if [ -z "$tag" ] || [ "$tag" = "qn" ]; then - # Make sure PCL for test is downloaded from S3 + # Make sure PCL for test is downloaded from S3. qn_name="1.tsv" qn_test_raw_dir="$volume_directory/QN" qn_test_data_1="$qn_test_raw_dir/$qn_name" @@ -436,7 +437,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_1" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="2.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -445,7 +446,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_2" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="3.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -454,7 +455,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_3" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="4.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -463,7 +464,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_4" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="5.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -472,7 +473,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_5" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="6.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -481,7 +482,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_6" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi qn_name="7.tsv" qn_test_raw_dir="$volume_directory/QN" @@ -490,7 +491,7 @@ if [ -z "$tag" ] || [ "$tag" = "qn" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for tests." wget -q -O "$qn_test_data_7" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi fi if [ -z "$tag" ] || [ "$tag" = "compendia" ]; then @@ -522,58 +523,61 @@ if [ -z "$tag" ] || [ "$tag" = "compendia" ]; then mkdir -p "$qn_test_raw_dir" echo "Downloading QN for compendia tests." wget -q -O "$qn_test_data_1" \ - "$test_data_repo/$qn_name" + "$test_data_repo/$qn_name" fi fi -. scripts/common.sh +. ./scripts/common.sh + DB_HOST_IP=$(get_docker_db_ip_address) # Ensure permissions are set for everything within the test data directory. chmod -R a+rwX "$volume_directory" -worker_images="salmon transcriptome no_op downloaders smasher illumina agilent affymetrix qn affymetrix_local janitor compendia" +worker_images="salmon transcriptome no_op downloaders smasher illumina agilent affymetrix qn janitor compendia" for image in $worker_images; do if [ -z "$tag" ] || [ "$tag" = "$image" ]; then if [ "$image" = "agilent" ] || [ "$image" = "affymetrix" ]; then - # Agilent uses the same docker image as Affymetrix - ./scripts/prepare_image.sh -p -i affymetrix -s workers - ./scripts/prepare_image.sh -i affymetrix_local -d ccdlstaging - docker tag ccdlstaging/dr_affymetrix_local:latest ccdlstaging/dr_affymetrix:latest - image_name=ccdlstaging/dr_affymetrix + # Agilent uses the same docker image as Affymetrix. + ./scripts/prepare_image.sh -i affymetrix -s workers + image_name="$DOCKERHUB_REPO/dr_affymetrix" elif [ "$tag" = "qn" ]; then ./scripts/prepare_image.sh -i smasher -s workers - image_name=ccdlstaging/dr_smasher + image_name="$DOCKERHUB_REPO/dr_smasher" elif [ "$tag" = "janitor" ]; then ./scripts/prepare_image.sh -i smasher -s workers - image_name=ccdlstaging/dr_smasher + image_name="$DOCKERHUB_REPO/dr_smasher" else ./scripts/prepare_image.sh -i "$image" -s workers - image_name=ccdlstaging/dr_$image + image_name="$DOCKERHUB_REPO/dr_$image" fi - # Strip out tag argument + # Strip out tag argument. # shellcheck disable=2001 args_without_tag="$(echo "$@" | sed "s/-t $tag//")" # shellcheck disable=2086 test_command="$(run_tests_with_coverage --tag="$image" $args_without_tag)" - # Only run interactively if we are on a TTY + # Only run interactively if we are on a TTY. if [ -t 1 ]; then - INTERACTIVE="-i" + INTERACTIVE="--interactive" fi echo "Running tests with the following command:" echo "$test_command" - docker run -t $INTERACTIVE \ - --add-host=database:"$DB_HOST_IP" \ - --env-file workers/environments/test \ - --env AWS_ACCESS_KEY_ID \ - --env AWS_SECRET_ACCESS_KEY \ - --memory=5G \ - --platform linux/amd64 \ - --volume "$volume_directory":/home/user/data_store \ - "$image_name" bash -c "$test_command" + # shellcheck disable=SC2086 + docker run \ + --add-host=database:"$DB_HOST_IP" \ + --env AWS_ACCESS_KEY_ID \ + --env AWS_SECRET_ACCESS_KEY \ + --env-file workers/environments/test \ + --memory=5G \ + --platform linux/amd64 \ + --tty \ + --volume "$volume_directory":/home/user/data_store \ + $INTERACTIVE \ + "$image_name" \ + bash -c "$test_command" fi done From ee4f563a0589b6f9761882013f6037ceb7bf877c Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 28 Jun 2023 17:11:11 -0400 Subject: [PATCH 140/220] linting --- .../data_refinery_foreman/surveyor/harmony.py | 94 +++++++++++-------- 1 file changed, 57 insertions(+), 37 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 433206de9..ba9725111 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -5,7 +5,7 @@ `sex`, `age`, `specimen_part`, - `genetic_information`, + `genotype`, `disease`, `disease_stage`, `cell_line`, @@ -245,6 +245,7 @@ """ import csv +import re from io import StringIO from typing import Dict, List @@ -253,6 +254,9 @@ logger = get_and_configure_logger(__name__) +# re.Pattern / re._pattern_type were not defined in 3.8? +RE_TYPE = re.compile("").__class__ + TITLE_FIELDS = [ "sample name", @@ -292,7 +296,7 @@ def create_variants(fields_list: List): Ex, given 'cell line', add the ability to hit on 'characteristic [cell_line]' as well. """ - variants = [] + variants = set() for field in fields_list: space_variants = [field] @@ -303,24 +307,27 @@ def create_variants(fields_list: List): space_variants.append(field.replace(" ", "")) for space_variant in space_variants: - variants.append(space_variant) - variants.append("characteristic [" + space_variant + "]") - variants.append("characteristic[" + space_variant + "]") - variants.append("characteristics [" + space_variant + "]") - variants.append("characteristics[" + space_variant + "]") - variants.append("comment [" + space_variant + "]") - variants.append("comment[" + space_variant + "]") - variants.append("comments [" + space_variant + "]") - variants.append("comments[" + space_variant + "]") - variants.append("factorvalue[" + space_variant + "]") - variants.append("factor value[" + space_variant + "]") - variants.append("factorvalue [" + space_variant + "]") - variants.append("factor value [" + space_variant + "]") - variants.append("sample_" + space_variant) - variants.append("sample_host" + space_variant) - variants.append("sample_sample_" + space_variant) # Yes, seriously. - - return variants + variants.add(space_variant) + variants.add("characteristic [" + space_variant + "]") + variants.add("characteristic[" + space_variant + "]") + variants.add("characteristics [" + space_variant + "]") + variants.add("characteristics[" + space_variant + "]") + variants.add("comment [" + space_variant + "]") + variants.add("comment[" + space_variant + "]") + variants.add("comments [" + space_variant + "]") + variants.add("comments[" + space_variant + "]") + variants.add("factorvalue[" + space_variant + "]") + variants.add("factor value[" + space_variant + "]") + variants.add("factorvalue [" + space_variant + "]") + variants.add("factor value [" + space_variant + "]") + variants.add("sample_" + space_variant) + variants.add("sample_host" + space_variant) + variants.add("sample_sample_" + space_variant) # Yes, seriously. + + # handle SRA flattened json response + variants.add(re.compile("characteristics_" + space_variant + "(?:_\d+_text)?$")) + + return list(variants) def parse_sdrf(sdrf_url: str) -> List: @@ -443,8 +450,7 @@ def __init__(self): "characteristic [organism part]", "characteristics [organism part]", # SRA - "cell_type", - "organismpart", + "organismpart", # this isnt seen? # GEO "isolation source", "tissue sampled", @@ -452,7 +458,7 @@ def __init__(self): ] self.specimen_part_fields = create_variants(specimen_part_fields) - genetic_information_fields = [ + genotype_fields = [ "strain/background", "strain", "strain or line", @@ -465,7 +471,7 @@ def __init__(self): "cultivar", "strain/genotype", ] - self.genetic_information_fields = create_variants(genetic_information_fields) + self.genotype_fields = create_variants(genotype_fields) disease_fields = [ "disease", @@ -493,8 +499,8 @@ def __init__(self): cell_line_fields = [ "cell line", - "sample strain", ] + self.cell_line_fields = create_variants(cell_line_fields) treatment_fields = [ @@ -531,7 +537,7 @@ def __init__(self): "donor id", "donor", # SRA - "sample_source_name", + # create_variants will find above attributes in characteristics ] self.subject_fields = create_variants(subject_fields) @@ -539,6 +545,8 @@ def __init__(self): "developmental stage", "development stage", "development stages", + # SRA. + "dev stage", ] self.developmental_stage_fields = create_variants(developmental_stage_fields) @@ -585,25 +593,37 @@ def harmonize_value(self, field_name: str, value): else: return value.lower().strip() - def harmonize_field(self, sample_metadata: Dict, harmonized_sample: Dict, field: str): - field_name = field.split("_fields")[0] - + def harmonize_field( + self, sample_metadata: Dict, harmonized_sample: Dict, variants_attribute: str + ): + sample_attribute = variants_attribute.split("_fields")[0] + harmonized_values = set() for key, value in sample_metadata.items(): lower_key = key.lower().strip() - if lower_key in getattr(self, field): - harmonized_value = self.harmonize_value(field_name, value) + key_variants = getattr(self, variants_attribute) + key_regexes = [r for r in key_variants if isinstance(r, RE_TYPE)] + key_strings = [s for s in key_variants if isinstance(s, str)] + if lower_key in key_strings: + harmonized_value = self.harmonize_value(sample_attribute, value) if harmonized_value: - harmonized_sample[field_name] = harmonized_value - break + harmonized_values.add(harmonized_value) + else: + for key_regex in key_regexes: + if key_regex.fullmatch(lower_key): + harmonized_value = self.harmonize_value(sample_attribute, value) + if harmonized_value: + harmonized_values.add(harmonized_value) + + harmonized_sample[sample_attribute] = ";".join([str(v) for v in harmonized_values]) def harmonize_sample(self, sample_metadata: Dict, title_field: str = None) -> Dict: - fields = [ + variants_attributes = [ "sex_fields", "age_fields", "specimen_part_fields", - "genetic_information_fields", + "genotype_fields", "disease_fields", "disease_stage_fields", "cell_line_fields", @@ -617,8 +637,8 @@ def harmonize_sample(self, sample_metadata: Dict, title_field: str = None) -> Di harmonized_sample = {} harmonized_sample["title"] = extract_title(sample_metadata, title_field) - for field in fields: - self.harmonize_field(sample_metadata, harmonized_sample, field) + for variants_attribute in variants_attributes: + self.harmonize_field(sample_metadata, harmonized_sample, variants_attribute) return harmonized_sample From dc16fecd31b0d5ef008708a74038e13ca1bc70de Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 28 Jun 2023 17:11:11 -0400 Subject: [PATCH 141/220] Intial Harmonizer changes for new ENA surveyor --- .../data_refinery_foreman/surveyor/harmony.py | 94 +++++++++++-------- 1 file changed, 57 insertions(+), 37 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 433206de9..ba9725111 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -5,7 +5,7 @@ `sex`, `age`, `specimen_part`, - `genetic_information`, + `genotype`, `disease`, `disease_stage`, `cell_line`, @@ -245,6 +245,7 @@ """ import csv +import re from io import StringIO from typing import Dict, List @@ -253,6 +254,9 @@ logger = get_and_configure_logger(__name__) +# re.Pattern / re._pattern_type were not defined in 3.8? +RE_TYPE = re.compile("").__class__ + TITLE_FIELDS = [ "sample name", @@ -292,7 +296,7 @@ def create_variants(fields_list: List): Ex, given 'cell line', add the ability to hit on 'characteristic [cell_line]' as well. """ - variants = [] + variants = set() for field in fields_list: space_variants = [field] @@ -303,24 +307,27 @@ def create_variants(fields_list: List): space_variants.append(field.replace(" ", "")) for space_variant in space_variants: - variants.append(space_variant) - variants.append("characteristic [" + space_variant + "]") - variants.append("characteristic[" + space_variant + "]") - variants.append("characteristics [" + space_variant + "]") - variants.append("characteristics[" + space_variant + "]") - variants.append("comment [" + space_variant + "]") - variants.append("comment[" + space_variant + "]") - variants.append("comments [" + space_variant + "]") - variants.append("comments[" + space_variant + "]") - variants.append("factorvalue[" + space_variant + "]") - variants.append("factor value[" + space_variant + "]") - variants.append("factorvalue [" + space_variant + "]") - variants.append("factor value [" + space_variant + "]") - variants.append("sample_" + space_variant) - variants.append("sample_host" + space_variant) - variants.append("sample_sample_" + space_variant) # Yes, seriously. - - return variants + variants.add(space_variant) + variants.add("characteristic [" + space_variant + "]") + variants.add("characteristic[" + space_variant + "]") + variants.add("characteristics [" + space_variant + "]") + variants.add("characteristics[" + space_variant + "]") + variants.add("comment [" + space_variant + "]") + variants.add("comment[" + space_variant + "]") + variants.add("comments [" + space_variant + "]") + variants.add("comments[" + space_variant + "]") + variants.add("factorvalue[" + space_variant + "]") + variants.add("factor value[" + space_variant + "]") + variants.add("factorvalue [" + space_variant + "]") + variants.add("factor value [" + space_variant + "]") + variants.add("sample_" + space_variant) + variants.add("sample_host" + space_variant) + variants.add("sample_sample_" + space_variant) # Yes, seriously. + + # handle SRA flattened json response + variants.add(re.compile("characteristics_" + space_variant + "(?:_\d+_text)?$")) + + return list(variants) def parse_sdrf(sdrf_url: str) -> List: @@ -443,8 +450,7 @@ def __init__(self): "characteristic [organism part]", "characteristics [organism part]", # SRA - "cell_type", - "organismpart", + "organismpart", # this isnt seen? # GEO "isolation source", "tissue sampled", @@ -452,7 +458,7 @@ def __init__(self): ] self.specimen_part_fields = create_variants(specimen_part_fields) - genetic_information_fields = [ + genotype_fields = [ "strain/background", "strain", "strain or line", @@ -465,7 +471,7 @@ def __init__(self): "cultivar", "strain/genotype", ] - self.genetic_information_fields = create_variants(genetic_information_fields) + self.genotype_fields = create_variants(genotype_fields) disease_fields = [ "disease", @@ -493,8 +499,8 @@ def __init__(self): cell_line_fields = [ "cell line", - "sample strain", ] + self.cell_line_fields = create_variants(cell_line_fields) treatment_fields = [ @@ -531,7 +537,7 @@ def __init__(self): "donor id", "donor", # SRA - "sample_source_name", + # create_variants will find above attributes in characteristics ] self.subject_fields = create_variants(subject_fields) @@ -539,6 +545,8 @@ def __init__(self): "developmental stage", "development stage", "development stages", + # SRA. + "dev stage", ] self.developmental_stage_fields = create_variants(developmental_stage_fields) @@ -585,25 +593,37 @@ def harmonize_value(self, field_name: str, value): else: return value.lower().strip() - def harmonize_field(self, sample_metadata: Dict, harmonized_sample: Dict, field: str): - field_name = field.split("_fields")[0] - + def harmonize_field( + self, sample_metadata: Dict, harmonized_sample: Dict, variants_attribute: str + ): + sample_attribute = variants_attribute.split("_fields")[0] + harmonized_values = set() for key, value in sample_metadata.items(): lower_key = key.lower().strip() - if lower_key in getattr(self, field): - harmonized_value = self.harmonize_value(field_name, value) + key_variants = getattr(self, variants_attribute) + key_regexes = [r for r in key_variants if isinstance(r, RE_TYPE)] + key_strings = [s for s in key_variants if isinstance(s, str)] + if lower_key in key_strings: + harmonized_value = self.harmonize_value(sample_attribute, value) if harmonized_value: - harmonized_sample[field_name] = harmonized_value - break + harmonized_values.add(harmonized_value) + else: + for key_regex in key_regexes: + if key_regex.fullmatch(lower_key): + harmonized_value = self.harmonize_value(sample_attribute, value) + if harmonized_value: + harmonized_values.add(harmonized_value) + + harmonized_sample[sample_attribute] = ";".join([str(v) for v in harmonized_values]) def harmonize_sample(self, sample_metadata: Dict, title_field: str = None) -> Dict: - fields = [ + variants_attributes = [ "sex_fields", "age_fields", "specimen_part_fields", - "genetic_information_fields", + "genotype_fields", "disease_fields", "disease_stage_fields", "cell_line_fields", @@ -617,8 +637,8 @@ def harmonize_sample(self, sample_metadata: Dict, title_field: str = None) -> Di harmonized_sample = {} harmonized_sample["title"] = extract_title(sample_metadata, title_field) - for field in fields: - self.harmonize_field(sample_metadata, harmonized_sample, field) + for variants_attribute in variants_attributes: + self.harmonize_field(sample_metadata, harmonized_sample, variants_attribute) return harmonized_sample From c26358aeeb8b8872d47e7a52119f6ea86350b97b Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 5 Jul 2023 19:31:05 -0700 Subject: [PATCH 142/220] Address review comments --- scripts/prepare_image.sh | 20 ++++++++++---------- scripts/update_docker_images.sh | 16 ++++++++-------- 2 files changed, 18 insertions(+), 18 deletions(-) diff --git a/scripts/prepare_image.sh b/scripts/prepare_image.sh index 4bdff97c4..d9a49c68d 100755 --- a/scripts/prepare_image.sh +++ b/scripts/prepare_image.sh @@ -91,14 +91,14 @@ fi DOCKERHUB_IMAGE="$DOCKERHUB_REPO/dr_$IMAGE_NAME" -CACHE_FROM_LATEST="cache-from=type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest" -CACHE_FROM_VERSION="cache-from=type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION" -CACHE_TO_LATEST="cache-to=type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" -CACHE_TO_VERSION="cache-to=type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" +CACHE_FROM_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest" +CACHE_FROM_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION" +CACHE_TO_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" +CACHE_TO_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" if test "$GITHUB_ACTION"; then - CACHE_TO_LATEST="cache-to=type=gha" - CACHE_TO_VERSION="cache-to=type=gha" + CACHE_TO_LATEST="type=gha" + CACHE_TO_VERSION="type=gha" DOCKER_ACTION="--push" fi @@ -121,10 +121,10 @@ while [ $finished != 0 ] && [ $attempt -lt $attempts ]; do docker buildx build \ --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ - --"$CACHE_FROM_LATEST" \ - --"$CACHE_FROM_VERSION" \ - --"$CACHE_TO_LATEST" \ - --"$CACHE_TO_VERSION" \ + --cache-from "$CACHE_FROM_LATEST" \ + --cache-from "$CACHE_FROM_VERSION" \ + --cache-to "$CACHE_TO_LATEST" \ + --cache-to "$CACHE_TO_VERSION" \ --file "$DOCKER_FILE_PATH" \ --platform linux/amd64 \ --tag "$DOCKERHUB_IMAGE:latest" \ diff --git a/scripts/update_docker_images.sh b/scripts/update_docker_images.sh index f051e8317..d3910854a 100755 --- a/scripts/update_docker_images.sh +++ b/scripts/update_docker_images.sh @@ -113,20 +113,20 @@ for image_name in $image_names; do echo DOCKERHUB_IMAGE="$DOCKERHUB_REPO/dr_$image_name" - CACHE_FROM_LATEST="cache-from=type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest" - CACHE_FROM_VERSION="cache-from=type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION" - CACHE_TO_LATEST="cache-to=type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" - CACHE_TO_VERSION="cache-to=type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" + CACHE_FROM_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest" + CACHE_FROM_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION" + CACHE_TO_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" + CACHE_TO_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" set_up_docker_builder docker buildx build \ --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ - --"$CACHE_FROM_LATEST" \ - --"$CACHE_FROM_VERSION" \ - --"$CACHE_TO_LATEST" \ - --"$CACHE_TO_VERSION" \ + --cache-from "$CACHE_FROM_LATEST" \ + --cache-from "$CACHE_FROM_VERSION" \ + --cache-to "$CACHE_TO_LATEST" \ + --cache-to "$CACHE_TO_VERSION" \ --file "$DOCKER_FILE_PATH" \ --platform linux/amd64 \ --push \ From 169d687a0d076e10b44eea3ca065bb6372f290a4 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 16:01:33 -0400 Subject: [PATCH 143/220] updating example SRA metadata comment --- .../data_refinery_foreman/surveyor/harmony.py | 382 +++++++++++++----- 1 file changed, 276 insertions(+), 106 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index ba9725111..2e8e50174 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -45,112 +45,282 @@ 'sex': 'male'} SRA Example: - {'alias': 'GSM2997959_r1', - 'broker_name': 'GEO', - 'center_name': 'GEO', - 'center_project_name': 'GSE99065', - 'ena-base-count': '279111754922', - 'ena-spot-count': '1152605026', - 'experiment_accession': 'SRX3691797', - 'experiment_design_description': None, - 'experiment_title': 'NextSeq 500 paired end sequencing; GSM2997959: INOF_FRT; ' - 'Homo sapiens; RNA-Seq', - 'lab_name': '', - 'library_construction_protocol': 'cDNA library produced with TGIRT RNA was ' - 'isolated and DNase treated using RNEasy ' - 'mini kit (Qiagen 74106) according to the ' - 'manufacturer protocol. 5ug DNA-free total ' - 'RNA was then ribodepleted using Ribo-zero ' - 'Gold (Illumina RZG1224 ) according to the ' - 'manufacturer protocol and purified using a ' - 'modified ZYMO RNA Clean and Concentrator ' - '(R1016) protocol where 8 volumes EtOH ' - 'instead of 4. rRNA depleted RNA was ' - 'fragmented with NEBNext Magnesium RNA ' - 'Fragmentation Module (E6150) followed by ' - 'dephosphorylation using T4PNK (mandel ) ' - 'and purified by same modified ZYMO ' - 'protocol. cDNAs were synthesized via TGIRT ' - 'template-switching with 1µM TGIRT-III ' - 'reverse transcriptase (Ingex, LLC) for 15 ' - 'min at 60o C, during which a DNA ' - 'oligonucleotide containing the complement ' - 'of an Illumina Read 2 sequencing ' - 'primer-binding site becomes seamlessly ' - "linked to the 5' cDNA end. After reaction " - 'cleanup (Qiagen MinElute Reaction cleanup ' - "28206), a 5' adenylated DNA oligonucleotide " - 'containing the complement of an Illumina ' - 'Read 1 sequencing primer-binding site is ' - "then ligated to the 3' cDNA end with " - "Thermostable 5' AppDNA / RNA Ligase (New " - 'England Biolabs M0319). Properly ligated ' - 'cDNAs were amplified by PCR (12 cycles) to ' - 'synthesize the second strand and add ' - 'Illumina flowcell capture and index ' - 'sequences. Library was size-selected with ' - 'Ampure XP beads (Beckman-Coulter) and ' - 'quantified with Qubit and evaluated on an ' - 'Agilent 2100 Bioanalyzer.', - 'library_layout': 'PAIRED', - 'library_selection': 'cDNA', - 'library_source': 'TRANSCRIPTOMIC', - 'library_strategy': 'RNA-Seq', - 'organism_id': '9606', - 'organism_name': 'HOMO SAPIENS', - 'platform_instrument_model': 'NextSeq500', - 'run_accession': 'SRR6718414', - 'run_ena_base_count': '1773379870', - 'run_ena_first_public': '2018-02-17', - 'run_ena_last_update': '2018-02-17', - 'run_ena_spot_count': '15046271', - 'sample_accession': 'SRS2951393', - 'sample_cell_type': 'Immortalized normal ovarian fibroblast', - 'sample_ena_base_count': '1773379870', - 'sample_ena_first_public': '2018-02-14', - 'sample_ena_last_update': '2018-02-14', - 'sample_ena_spot_count': '15046271', - 'sample_source_name': 'INOF cell line', - 'sample_title': 'INOF_FRT', - 'sample_treatment': 'none', - 'study_abstract': 'The ability to compare the abundance of one RNA molecule ' - 'to another is a crucial step for understanding how gene ' - 'expression is modulated to shape the transcriptome ' - 'landscape. However, little information is available about ' - 'the relative expression of the different classes of coding ' - 'and non-coding RNA or even between RNA of the same class. ' - 'In this study, we present a complete portrait of the human ' - 'transcriptome that depicts the relationship of all classes ' - 'of non-ribosomal RNA longer than sixty nucleotides. The ' - 'results show that the most abundant RNA in the human ' - 'rRNA-depleted transcriptome is tRNA followed by ' - 'spliceosomal RNA. Surprisingly, the signal recognition ' - 'particle RNA 7SL by itself occupied 8% of the ribodepleted ' - 'transcriptome producing a similar number of transcripts as ' - 'that produced by all snoRNA genes combined. In general, ' - 'the most abundant RNA are non-coding but many more protein ' - 'coding than non-coding genes produce more than 1 ' - 'transcript per million. Examination of gene functions ' - 'suggests that RNA abundance reflects both gene and cell ' - 'function. Together, the data indicate that the human ' - 'transcriptome is shaped by a small number of highly ' - 'expressed non-coding genes and a large number of ' - 'moderately expressed protein coding genes that reflect ' - 'cellular phenotypes. Overall design: RNA was isolated from ' - 'SKOV3ip1 and INOF human cell lines and selected with ' - 'different methods. 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LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T-cells and NK-like and a minor one of myeloid-like cells. Single cell transcriptomes are matched with FACS index sorting data (GFP, forward and side light scatter and dead cell staining)", + "study_ena_secondary_study_alias": "E-MTAB-4617", + "study_ena_scientific_name": "", + "study_ena_study_alias": "E-MTAB-4617", + "study_ena_breed": "", + "study_ena_tax_id": "", + "study_ena_parent_study_accession": "", + "study_ena_study_accession": "PRJEB14175", + "study_ena_submission_tool": "", + "study_ena_cultivar": "", + "study_ena_tax_division": "", + "study_ena_status": "public"} GEO: ex: From af81393a8b91f83186fcd659da1ea2940006f398 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 16:13:55 -0400 Subject: [PATCH 144/220] move separating strings and regex out of loop --- foreman/data_refinery_foreman/surveyor/harmony.py | 9 ++++----- 1 file changed, 4 insertions(+), 5 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 2e8e50174..888302b5b 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -768,13 +768,12 @@ def harmonize_field( ): sample_attribute = variants_attribute.split("_fields")[0] harmonized_values = set() + key_variants = getattr(self, variants_attribute) + key_regexes = [r for r in key_variants if isinstance(r, RE_TYPE)] + key_strings = [s for s in key_variants if isinstance(s, str)] + for key, value in sample_metadata.items(): lower_key = key.lower().strip() - - key_variants = getattr(self, variants_attribute) - key_regexes = [r for r in key_variants if isinstance(r, RE_TYPE)] - key_strings = [s for s in key_variants if isinstance(s, str)] - if lower_key in key_strings: harmonized_value = self.harmonize_value(sample_attribute, value) if harmonized_value: From 04db2c7e30d379b08062da82d224a7c7956cb0c1 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 17:07:00 -0400 Subject: [PATCH 145/220] always do regex checks and limit age to one value --- .../data_refinery_foreman/surveyor/harmony.py | 18 +++++++++++------- 1 file changed, 11 insertions(+), 7 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 888302b5b..ffe4b9c84 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -778,14 +778,18 @@ def harmonize_field( harmonized_value = self.harmonize_value(sample_attribute, value) if harmonized_value: harmonized_values.add(harmonized_value) - else: - for key_regex in key_regexes: - if key_regex.fullmatch(lower_key): - harmonized_value = self.harmonize_value(sample_attribute, value) - if harmonized_value: - harmonized_values.add(harmonized_value) - harmonized_sample[sample_attribute] = ";".join([str(v) for v in harmonized_values]) + for key_regex in key_regexes: + if key_regex.fullmatch(lower_key): + harmonized_value = self.harmonize_value(sample_attribute, value) + if harmonized_value: + harmonized_values.add(harmonized_value) + + # Age can only be one value in the database. + if sample_attribute == "age" and len(harmonized_values) > 0: + harmonized_sample[sample_attribute] = harmonized_values.pop() + else: + harmonized_sample[sample_attribute] = ";".join([str(v) for v in harmonized_values]) def harmonize_sample(self, sample_metadata: Dict, title_field: str = None) -> Dict: variants_attributes = [ From 8810e9d7046d18bb7ea0eedcaf6ef7287ec38a26 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 17:15:00 -0400 Subject: [PATCH 146/220] add back source name for subject --- foreman/data_refinery_foreman/surveyor/harmony.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index ffe4b9c84..a668351ca 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -707,7 +707,7 @@ def __init__(self): "donor id", "donor", # SRA - # create_variants will find above attributes in characteristics + "source name", ] self.subject_fields = create_variants(subject_fields) From a1361321ff9ac9852e2cf1726f4053a7c20c4afc Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 17:26:00 -0400 Subject: [PATCH 147/220] update harmonizer --- foreman/tests/surveyor/test_sra.py | 12 +++++++----- 1 file changed, 7 insertions(+), 5 deletions(-) diff --git a/foreman/tests/surveyor/test_sra.py b/foreman/tests/surveyor/test_sra.py index 1a098409a..4a6a89234 100644 --- a/foreman/tests/surveyor/test_sra.py +++ b/foreman/tests/surveyor/test_sra.py @@ -261,19 +261,21 @@ def test_metadata_is_gathered_correctly(self): ) def test_sra_metadata_is_harmonized(self): - metadata = SraSurveyor.gather_all_metadata("SRR3098582") + experiment_metadata, samples_metadata = SraSurveyor.gather_all_metadata("SRR3098582") + sample_metadata = samples_metadata[0] + sample = Sample() - SraSurveyor._apply_harmonized_metadata_to_sample(sample, metadata) + SraSurveyor._apply_harmonized_metadata_to_sample(sample, sample_metadata) self.assertEqual(sample.treatment, "biliatresone") self.assertEqual(sample.subject, "liver") self.assertEqual(sample.specimen_part, "liver") experiment = Experiment() - SraSurveyor._apply_metadata_to_experiment(experiment, metadata) + SraSurveyor._apply_metadata_to_experiment(experiment, experiment_metadata) self.assertEqual( experiment.title, "Transcriptional profiling through RNA-seq of zebrafish larval" " liver after exposure to biliatresone, a biliary toxin.", ) - self.assertEqual(experiment.source_first_published, datetime.date(2017, 9, 25)) - self.assertEqual(experiment.source_last_modified, datetime.date(2017, 9, 25)) + self.assertEqual(experiment.source_first_published, datetime.date(2017, 8, 25)) + self.assertEqual(experiment.source_last_modified, datetime.date(2023, 5, 19)) From 908c67e552bf233ae4683608d07628cf70b93672 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 17:36:16 -0400 Subject: [PATCH 148/220] remove redundant organismpart field --- foreman/data_refinery_foreman/surveyor/harmony.py | 2 -- 1 file changed, 2 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index a668351ca..123ef5236 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -619,8 +619,6 @@ def __init__(self): "organ", "characteristic [organism part]", "characteristics [organism part]", - # SRA - "organismpart", # this isnt seen? # GEO "isolation source", "tissue sampled", From 53a5663c2b4baf9cae34e011606b1435e241d757 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 28 Jun 2023 17:11:11 -0400 Subject: [PATCH 149/220] Intial Harmonizer changes for new ENA surveyor --- .../data_refinery_foreman/surveyor/harmony.py | 94 +++++++++++-------- 1 file changed, 57 insertions(+), 37 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 433206de9..ba9725111 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -5,7 +5,7 @@ `sex`, `age`, `specimen_part`, - `genetic_information`, + `genotype`, `disease`, `disease_stage`, `cell_line`, @@ -245,6 +245,7 @@ """ import csv +import re from io import StringIO from typing import Dict, List @@ -253,6 +254,9 @@ logger = get_and_configure_logger(__name__) +# re.Pattern / re._pattern_type were not defined in 3.8? +RE_TYPE = re.compile("").__class__ + TITLE_FIELDS = [ "sample name", @@ -292,7 +296,7 @@ def create_variants(fields_list: List): Ex, given 'cell line', add the ability to hit on 'characteristic [cell_line]' as well. """ - variants = [] + variants = set() for field in fields_list: space_variants = [field] @@ -303,24 +307,27 @@ def create_variants(fields_list: List): space_variants.append(field.replace(" ", "")) for space_variant in space_variants: - variants.append(space_variant) - variants.append("characteristic [" + space_variant + "]") - variants.append("characteristic[" + space_variant + "]") - variants.append("characteristics [" + space_variant + "]") - variants.append("characteristics[" + space_variant + "]") - variants.append("comment [" + space_variant + "]") - variants.append("comment[" + space_variant + "]") - variants.append("comments [" + space_variant + "]") - variants.append("comments[" + space_variant + "]") - variants.append("factorvalue[" + space_variant + "]") - variants.append("factor value[" + space_variant + "]") - variants.append("factorvalue [" + space_variant + "]") - variants.append("factor value [" + space_variant + "]") - variants.append("sample_" + space_variant) - variants.append("sample_host" + space_variant) - variants.append("sample_sample_" + space_variant) # Yes, seriously. - - return variants + variants.add(space_variant) + variants.add("characteristic [" + space_variant + "]") + variants.add("characteristic[" + space_variant + "]") + variants.add("characteristics [" + space_variant + "]") + variants.add("characteristics[" + space_variant + "]") + variants.add("comment [" + space_variant + "]") + variants.add("comment[" + space_variant + "]") + variants.add("comments [" + space_variant + "]") + variants.add("comments[" + space_variant + "]") + variants.add("factorvalue[" + space_variant + "]") + variants.add("factor value[" + space_variant + "]") + variants.add("factorvalue [" + space_variant + "]") + variants.add("factor value [" + space_variant + "]") + variants.add("sample_" + space_variant) + variants.add("sample_host" + space_variant) + variants.add("sample_sample_" + space_variant) # Yes, seriously. + + # handle SRA flattened json response + variants.add(re.compile("characteristics_" + space_variant + "(?:_\d+_text)?$")) + + return list(variants) def parse_sdrf(sdrf_url: str) -> List: @@ -443,8 +450,7 @@ def __init__(self): "characteristic [organism part]", "characteristics [organism part]", # SRA - "cell_type", - "organismpart", + "organismpart", # this isnt seen? # GEO "isolation source", "tissue sampled", @@ -452,7 +458,7 @@ def __init__(self): ] self.specimen_part_fields = create_variants(specimen_part_fields) - genetic_information_fields = [ + genotype_fields = [ "strain/background", "strain", "strain or line", @@ -465,7 +471,7 @@ def __init__(self): "cultivar", "strain/genotype", ] - self.genetic_information_fields = create_variants(genetic_information_fields) + self.genotype_fields = create_variants(genotype_fields) disease_fields = [ "disease", @@ -493,8 +499,8 @@ def __init__(self): cell_line_fields = [ "cell line", - "sample strain", ] + self.cell_line_fields = create_variants(cell_line_fields) treatment_fields = [ @@ -531,7 +537,7 @@ def __init__(self): "donor id", "donor", # SRA - "sample_source_name", + # create_variants will find above attributes in characteristics ] self.subject_fields = create_variants(subject_fields) @@ -539,6 +545,8 @@ def __init__(self): "developmental stage", "development stage", "development stages", + # SRA. + "dev stage", ] self.developmental_stage_fields = create_variants(developmental_stage_fields) @@ -585,25 +593,37 @@ def harmonize_value(self, field_name: str, value): else: return value.lower().strip() - def harmonize_field(self, sample_metadata: Dict, harmonized_sample: Dict, field: str): - field_name = field.split("_fields")[0] - + def harmonize_field( + self, sample_metadata: Dict, harmonized_sample: Dict, variants_attribute: str + ): + sample_attribute = variants_attribute.split("_fields")[0] + harmonized_values = set() for key, value in sample_metadata.items(): lower_key = key.lower().strip() - if lower_key in getattr(self, field): - harmonized_value = self.harmonize_value(field_name, value) + key_variants = getattr(self, variants_attribute) + key_regexes = [r for r in key_variants if isinstance(r, RE_TYPE)] + key_strings = [s for s in key_variants if isinstance(s, str)] + if lower_key in key_strings: + harmonized_value = self.harmonize_value(sample_attribute, value) if harmonized_value: - harmonized_sample[field_name] = harmonized_value - break + harmonized_values.add(harmonized_value) + else: + for key_regex in key_regexes: + if key_regex.fullmatch(lower_key): + harmonized_value = self.harmonize_value(sample_attribute, value) + if harmonized_value: + harmonized_values.add(harmonized_value) + + harmonized_sample[sample_attribute] = ";".join([str(v) for v in harmonized_values]) def harmonize_sample(self, sample_metadata: Dict, title_field: str = None) -> Dict: - fields = [ + variants_attributes = [ "sex_fields", "age_fields", "specimen_part_fields", - "genetic_information_fields", + "genotype_fields", "disease_fields", "disease_stage_fields", "cell_line_fields", @@ -617,8 +637,8 @@ def harmonize_sample(self, sample_metadata: Dict, title_field: str = None) -> Di harmonized_sample = {} harmonized_sample["title"] = extract_title(sample_metadata, title_field) - for field in fields: - self.harmonize_field(sample_metadata, harmonized_sample, field) + for variants_attribute in variants_attributes: + self.harmonize_field(sample_metadata, harmonized_sample, variants_attribute) return harmonized_sample From 7721edb3af30a1eb15d4f006b9dd0450cf329648 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 16:01:33 -0400 Subject: [PATCH 150/220] updating example SRA metadata comment --- .../data_refinery_foreman/surveyor/harmony.py | 382 +++++++++++++----- 1 file changed, 276 insertions(+), 106 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index ba9725111..2e8e50174 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -45,112 +45,282 @@ 'sex': 'male'} SRA Example: - {'alias': 'GSM2997959_r1', - 'broker_name': 'GEO', - 'center_name': 'GEO', - 'center_project_name': 'GSE99065', - 'ena-base-count': '279111754922', - 'ena-spot-count': '1152605026', - 'experiment_accession': 'SRX3691797', - 'experiment_design_description': None, - 'experiment_title': 'NextSeq 500 paired end sequencing; GSM2997959: INOF_FRT; ' - 'Homo sapiens; RNA-Seq', - 'lab_name': '', - 'library_construction_protocol': 'cDNA library produced with TGIRT RNA was ' - 'isolated and DNase treated using RNEasy ' - 'mini kit (Qiagen 74106) according to the ' - 'manufacturer protocol. 5ug DNA-free total ' - 'RNA was then ribodepleted using Ribo-zero ' - 'Gold (Illumina RZG1224 ) according to the ' - 'manufacturer protocol and purified using a ' - 'modified ZYMO RNA Clean and Concentrator ' - '(R1016) protocol where 8 volumes EtOH ' - 'instead of 4. rRNA depleted RNA was ' - 'fragmented with NEBNext Magnesium RNA ' - 'Fragmentation Module (E6150) followed by ' - 'dephosphorylation using T4PNK (mandel ) ' - 'and purified by same modified ZYMO ' - 'protocol. cDNAs were synthesized via TGIRT ' - 'template-switching with 1µM TGIRT-III ' - 'reverse transcriptase (Ingex, LLC) for 15 ' - 'min at 60o C, during which a DNA ' - 'oligonucleotide containing the complement ' - 'of an Illumina Read 2 sequencing ' - 'primer-binding site becomes seamlessly ' - "linked to the 5' cDNA end. After reaction " - 'cleanup (Qiagen MinElute Reaction cleanup ' - "28206), a 5' adenylated DNA oligonucleotide " - 'containing the complement of an Illumina ' - 'Read 1 sequencing primer-binding site is ' - "then ligated to the 3' cDNA end with " - "Thermostable 5' AppDNA / RNA Ligase (New " - 'England Biolabs M0319). Properly ligated ' - 'cDNAs were amplified by PCR (12 cycles) to ' - 'synthesize the second strand and add ' - 'Illumina flowcell capture and index ' - 'sequences. Library was size-selected with ' - 'Ampure XP beads (Beckman-Coulter) and ' - 'quantified with Qubit and evaluated on an ' - 'Agilent 2100 Bioanalyzer.', - 'library_layout': 'PAIRED', - 'library_selection': 'cDNA', - 'library_source': 'TRANSCRIPTOMIC', - 'library_strategy': 'RNA-Seq', - 'organism_id': '9606', - 'organism_name': 'HOMO SAPIENS', - 'platform_instrument_model': 'NextSeq500', - 'run_accession': 'SRR6718414', - 'run_ena_base_count': '1773379870', - 'run_ena_first_public': '2018-02-17', - 'run_ena_last_update': '2018-02-17', - 'run_ena_spot_count': '15046271', - 'sample_accession': 'SRS2951393', - 'sample_cell_type': 'Immortalized normal ovarian fibroblast', - 'sample_ena_base_count': '1773379870', - 'sample_ena_first_public': '2018-02-14', - 'sample_ena_last_update': '2018-02-14', - 'sample_ena_spot_count': '15046271', - 'sample_source_name': 'INOF cell line', - 'sample_title': 'INOF_FRT', - 'sample_treatment': 'none', - 'study_abstract': 'The ability to compare the abundance of one RNA molecule ' - 'to another is a crucial step for understanding how gene ' - 'expression is modulated to shape the transcriptome ' - 'landscape. However, little information is available about ' - 'the relative expression of the different classes of coding ' - 'and non-coding RNA or even between RNA of the same class. ' - 'In this study, we present a complete portrait of the human ' - 'transcriptome that depicts the relationship of all classes ' - 'of non-ribosomal RNA longer than sixty nucleotides. The ' - 'results show that the most abundant RNA in the human ' - 'rRNA-depleted transcriptome is tRNA followed by ' - 'spliceosomal RNA. Surprisingly, the signal recognition ' - 'particle RNA 7SL by itself occupied 8% of the ribodepleted ' - 'transcriptome producing a similar number of transcripts as ' - 'that produced by all snoRNA genes combined. In general, ' - 'the most abundant RNA are non-coding but many more protein ' - 'coding than non-coding genes produce more than 1 ' - 'transcript per million. Examination of gene functions ' - 'suggests that RNA abundance reflects both gene and cell ' - 'function. Together, the data indicate that the human ' - 'transcriptome is shaped by a small number of highly ' - 'expressed non-coding genes and a large number of ' - 'moderately expressed protein coding genes that reflect ' - 'cellular phenotypes. Overall design: RNA was isolated from ' - 'SKOV3ip1 and INOF human cell lines and selected with ' - 'different methods. 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A non-transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide (PI) staining was used to exclude dead cells. Individual cells were sorted, using a Becton Dickinson Influx sorter with 488- and 561 nm lasers (Schulte et al., 2015) and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X-100 supplemented with 1 U/uL SUPERase In RNAse inhibitor (Ambion). The size, granularity and level of fluorescence for each cell were simultaneously recorded. The Smart-seq2 protocol (Picelli et al., 2014) was used to amplify the whole transcriptome and prepare libraries. 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A non-transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide (PI) staining was used to exclude dead cells. Individual cells were sorted, using a Becton Dickinson Influx sorter with 488- and 561 nm lasers (Schulte et al., 2015) and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X-100 supplemented with 1 U/uL SUPERase In RNAse inhibitor (Ambion). The size, granularity and level of fluorescence for each cell were simultaneously recorded. The Smart-seq2 protocol (Picelli et al., 2014) was used to amplify the whole transcriptome and prepare libraries. Twenty-five cycles of PCR amplification were performed.", + "characteristics_fsc_0_text": "23555", + "characteristics_fsc_0_tag": "attribute", + "characteristics_gfp_0_text": "109", + "characteristics_gfp_0_tag": "attribute", + "characteristics_individual_0_text": "2", + "characteristics_individual_0_tag": "attribute", + "characteristics_organism_0_text": "Danio rerio", + "characteristics_organism_0_ontologyTerms_0": "http://purl.obolibrary.org/obo/NCBITaxon_7955", + "characteristics_organism_1_text": "Danio rerio", + "characteristics_organism_1_ontologyTerms_0": "http://purl.obolibrary.org/obo/NCBITaxon_7955", + "characteristics_organism_1_tag": "attribute", + "characteristics_pi_0_text": "9", + "characteristics_pi_0_tag": "attribute", + "characteristics_plate_0_text": "4", + "characteristics_plate_0_tag": "attribute", + "characteristics_ssc_0_text": "10994", + "characteristics_ssc_0_tag": "attribute", + "characteristics_tissue_0_text": "Spleen", + "characteristics_tissue_0_ontologyTerms_0": "http://purl.obolibrary.org/obo/UBERON_0002106", + "characteristics_tissue_0_tag": "attribute", + "characteristics_title_0_text": "LCK_4#90", + "characteristics_well_0_text": "B12", + "characteristics_well_0_tag": "attribute", + "relationships_0_source": "SAMEG321352", + "relationships_0_type": "has member", + "relationships_0_target": "SAMEA4011859", + "externalReferences_0_url": "https://www.ebi.ac.uk/ena/browser/view/SAMEA4011859", + "submittedVia": "JSON_API", + "create": "2018-12-07T06:18:17.244Z", + "_links_self_href": "https://www.ebi.ac.uk/biosamples/samples/SAMEA4011859", + "_links_curationDomain_href": "https://www.ebi.ac.uk/biosamples/samples/SAMEA4011859{?curationdomain}", + "_links_curationDomain_templated": true, + "_links_curationLinks_href": "https://www.ebi.ac.uk/biosamples/samples/SAMEA4011859/curationlinks", + "_links_curationLink_href": "https://www.ebi.ac.uk/biosamples/samples/SAMEA4011859/curationlinks/{hash}", + "_links_curationLink_templated": true, + "_links_structuredData_href": "https://www.ebi.ac.uk/biosamples/structureddata/SAMEA4011859", + "study_ena_accession": "PRJEB14175", + "study_ena_keywords": "", + "study_ena_description": "Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T-cells and NK-like and a minor one of myeloid-like cells. Single cell transcriptomes are matched with FACS index sorting data (GFP, forward and side light scatter and dead cell staining)", + "study_ena_study_name": "Single-cell RNA sequencing of spleen-derived LCK cells from adult Zebrafish", + "study_ena_project_name": "Single-cell RNA sequencing of spleen-derived LCK cells from adult Zebrafish", + "study_ena_geo_accession": "", + "study_ena_isolate": "", + "study_ena_center_name": "Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics", + "study_ena_secondary_study_accession": "ERP015799", + "study_ena_tag": "ena", + "study_ena_secondary_study_center_name": "", + "study_ena_strain": "", + "study_ena_study_title": "Single-cell RNA sequencing of spleen-derived LCK cells from adult Zebrafish", + "study_ena_last_updated": "2016-05-26", + "study_ena_first_public": "2017-02-03", + "study_ena_broker_name": "ArrayExpress", + "study_ena_study_description": "Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T-cells and NK-like and a minor one of myeloid-like cells. Single cell transcriptomes are matched with FACS index sorting data (GFP, forward and side light scatter and dead cell staining)", + "study_ena_secondary_study_alias": "E-MTAB-4617", + "study_ena_scientific_name": "", + "study_ena_study_alias": "E-MTAB-4617", + "study_ena_breed": "", + "study_ena_tax_id": "", + "study_ena_parent_study_accession": "", + "study_ena_study_accession": "PRJEB14175", + "study_ena_submission_tool": "", + "study_ena_cultivar": "", + "study_ena_tax_division": "", + "study_ena_status": "public"} GEO: ex: From 95cdac5c75082f6a798ab557b29cc18d7738f78c Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 16:13:55 -0400 Subject: [PATCH 151/220] move separating strings and regex out of loop --- foreman/data_refinery_foreman/surveyor/harmony.py | 9 ++++----- 1 file changed, 4 insertions(+), 5 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 2e8e50174..888302b5b 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -768,13 +768,12 @@ def harmonize_field( ): sample_attribute = variants_attribute.split("_fields")[0] harmonized_values = set() + key_variants = getattr(self, variants_attribute) + key_regexes = [r for r in key_variants if isinstance(r, RE_TYPE)] + key_strings = [s for s in key_variants if isinstance(s, str)] + for key, value in sample_metadata.items(): lower_key = key.lower().strip() - - key_variants = getattr(self, variants_attribute) - key_regexes = [r for r in key_variants if isinstance(r, RE_TYPE)] - key_strings = [s for s in key_variants if isinstance(s, str)] - if lower_key in key_strings: harmonized_value = self.harmonize_value(sample_attribute, value) if harmonized_value: From a47ae6e3c9020de588d4e3919018f06e8800e773 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 17:07:00 -0400 Subject: [PATCH 152/220] always do regex checks and limit age to one value --- .../data_refinery_foreman/surveyor/harmony.py | 18 +++++++++++------- 1 file changed, 11 insertions(+), 7 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 888302b5b..ffe4b9c84 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -778,14 +778,18 @@ def harmonize_field( harmonized_value = self.harmonize_value(sample_attribute, value) if harmonized_value: harmonized_values.add(harmonized_value) - else: - for key_regex in key_regexes: - if key_regex.fullmatch(lower_key): - harmonized_value = self.harmonize_value(sample_attribute, value) - if harmonized_value: - harmonized_values.add(harmonized_value) - harmonized_sample[sample_attribute] = ";".join([str(v) for v in harmonized_values]) + for key_regex in key_regexes: + if key_regex.fullmatch(lower_key): + harmonized_value = self.harmonize_value(sample_attribute, value) + if harmonized_value: + harmonized_values.add(harmonized_value) + + # Age can only be one value in the database. + if sample_attribute == "age" and len(harmonized_values) > 0: + harmonized_sample[sample_attribute] = harmonized_values.pop() + else: + harmonized_sample[sample_attribute] = ";".join([str(v) for v in harmonized_values]) def harmonize_sample(self, sample_metadata: Dict, title_field: str = None) -> Dict: variants_attributes = [ From e174f0c3f4e80f0f33d54bef92565291b86e700d Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 17:15:00 -0400 Subject: [PATCH 153/220] add back source name for subject --- foreman/data_refinery_foreman/surveyor/harmony.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index ffe4b9c84..a668351ca 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -707,7 +707,7 @@ def __init__(self): "donor id", "donor", # SRA - # create_variants will find above attributes in characteristics + "source name", ] self.subject_fields = create_variants(subject_fields) From 7e37c5b09d3c65bba4c582f606070a0fa6fe96e8 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 17:26:00 -0400 Subject: [PATCH 154/220] update harmonizer --- foreman/tests/surveyor/test_sra.py | 12 +++++++----- 1 file changed, 7 insertions(+), 5 deletions(-) diff --git a/foreman/tests/surveyor/test_sra.py b/foreman/tests/surveyor/test_sra.py index 1a098409a..4a6a89234 100644 --- a/foreman/tests/surveyor/test_sra.py +++ b/foreman/tests/surveyor/test_sra.py @@ -261,19 +261,21 @@ def test_metadata_is_gathered_correctly(self): ) def test_sra_metadata_is_harmonized(self): - metadata = SraSurveyor.gather_all_metadata("SRR3098582") + experiment_metadata, samples_metadata = SraSurveyor.gather_all_metadata("SRR3098582") + sample_metadata = samples_metadata[0] + sample = Sample() - SraSurveyor._apply_harmonized_metadata_to_sample(sample, metadata) + SraSurveyor._apply_harmonized_metadata_to_sample(sample, sample_metadata) self.assertEqual(sample.treatment, "biliatresone") self.assertEqual(sample.subject, "liver") self.assertEqual(sample.specimen_part, "liver") experiment = Experiment() - SraSurveyor._apply_metadata_to_experiment(experiment, metadata) + SraSurveyor._apply_metadata_to_experiment(experiment, experiment_metadata) self.assertEqual( experiment.title, "Transcriptional profiling through RNA-seq of zebrafish larval" " liver after exposure to biliatresone, a biliary toxin.", ) - self.assertEqual(experiment.source_first_published, datetime.date(2017, 9, 25)) - self.assertEqual(experiment.source_last_modified, datetime.date(2017, 9, 25)) + self.assertEqual(experiment.source_first_published, datetime.date(2017, 8, 25)) + self.assertEqual(experiment.source_last_modified, datetime.date(2023, 5, 19)) From ac09e2a022a181a2bff136a343f91d77fe7f0971 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 7 Jul 2023 17:36:16 -0400 Subject: [PATCH 155/220] remove redundant organismpart field --- foreman/data_refinery_foreman/surveyor/harmony.py | 2 -- 1 file changed, 2 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index a668351ca..123ef5236 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -619,8 +619,6 @@ def __init__(self): "organ", "characteristic [organism part]", "characteristics [organism part]", - # SRA - "organismpart", # this isnt seen? # GEO "isolation source", "tissue sampled", From 9cf58e39b5bc14ec4357811bd758adb0c24c84d5 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 10 Jul 2023 17:28:43 -0700 Subject: [PATCH 156/220] Optimize Github Action scripts - Refactor scripts to use `common::update_docker_image()` - Use GH codecov action instead of `codecov.sh` - Delete no longer needed files (cleanup_instance.sh, codecov.sh, pull_docker_image.sh) - Use `set -e` consistently --- .github/scripts/cleanup_instance.sh | 27 - .github/scripts/codecov.sh | 1660 ----------------- .github/scripts/filter_tests.sh | 4 +- .github/scripts/post_deploy_cleanup.sh | 4 +- ...t_coverage.sh => prepare_test_coverage.sh} | 10 +- .github/scripts/pull_docker_images.sh | 16 - .github/scripts/push_docker_images.sh | 4 +- .github/scripts/remote_deploy.sh | 33 +- .github/scripts/run_terraform.sh | 17 +- ..._ca_certs.sh => update_ca_certificates.sh} | 0 .github/scripts/update_docker_image.sh | 85 - .github/scripts/update_docker_images.sh | 55 + .github/workflows/config.yml | 75 +- scripts/common.sh | 52 +- scripts/prepare_image.sh | 41 +- scripts/update_docker_images.sh | 48 +- 16 files changed, 219 insertions(+), 1912 deletions(-) delete mode 100755 .github/scripts/cleanup_instance.sh delete mode 100755 .github/scripts/codecov.sh rename .github/scripts/{upload_test_coverage.sh => prepare_test_coverage.sh} (72%) delete mode 100755 .github/scripts/pull_docker_images.sh rename .github/scripts/{fix_ca_certs.sh => update_ca_certificates.sh} (100%) delete mode 100755 .github/scripts/update_docker_image.sh create mode 100755 .github/scripts/update_docker_images.sh diff --git a/.github/scripts/cleanup_instance.sh b/.github/scripts/cleanup_instance.sh deleted file mode 100755 index d359f6966..000000000 --- a/.github/scripts/cleanup_instance.sh +++ /dev/null @@ -1,27 +0,0 @@ -#!/bin/bash - -rm -rf /usr/local/android-sdk-linux -rm -rf /usr/local/go -rm -rf /usr/local/android-ndk -rm -rf /home/ubuntu/nvm -rm -rf /home/ubuntu/.android -rm -rf /home/ubuntu/.phpenv -rm -rf /home/ubuntu/.rvm - -# Suggestions from https://github.com/actions/virtual-environments/issues/709#issuecomment-615370473 -sudo apt-get remove -y '^ghc-8.*' -sudo apt-get remove -y '^dotnet-.*' -sudo apt-get remove -y '^llvm-.*' -sudo apt-get remove -y 'php.*' -sudo apt-get remove -y \ - azure-cli \ - firefox \ - google-chrome-stable \ - google-cloud-sdk \ - hhvm \ - mono-devel \ - powershell -sudo apt-get autoremove -y -sudo apt-get clean - -rm -rf /usr/share/dotnet/ diff --git a/.github/scripts/codecov.sh b/.github/scripts/codecov.sh deleted file mode 100755 index 8648ee4d2..000000000 --- a/.github/scripts/codecov.sh +++ /dev/null @@ -1,1660 +0,0 @@ -#!/usr/bin/env bash - -# Apache License Version 2.0, January 2004 -# https://github.com/codecov/codecov-bash/blob/master/LICENSE - - -set -e +o pipefail - -VERSION="20191211-b8db533" - -url="https://codecov.io" -env="$CODECOV_ENV" -service="" -token="" -search_in="" -flags="" -exit_with=0 -curlargs="" -curlawsargs="" -dump="0" -clean="0" -curl_s="-s" -name="$CODECOV_NAME" -include_cov="" -exclude_cov="" -ddp="$(echo ~)/Library/Developer/Xcode/DerivedData" -xp="" -files="" -cacert="$CODECOV_CA_BUNDLE" -gcov_ignore="-not -path './bower_components/**' -not -path './node_modules/**' -not -path './vendor/**'" -gcov_include="" - -ft_gcov="1" -ft_coveragepy="1" -ft_fix="1" -ft_search="1" -ft_s3="1" -ft_network="1" -ft_xcodellvm="1" -ft_xcodeplist="0" -ft_gcovout="1" - -_git_root=$(git rev-parse --show-toplevel 2>/dev/null || hg root 2>/dev/null || echo $PWD) -git_root="$_git_root" -codecov_yml="" -remote_addr="" -if [ "$git_root" = "$PWD" ]; -then - git_root="." -fi - -url_o="" -pr_o="" -build_o="" -commit_o="" -search_in_o="" -tag_o="" -branch_o="" -slug_o="" -prefix_o="" - -commit="$VCS_COMMIT_ID" -branch="$VCS_BRANCH_NAME" -pr="$VCS_PULL_REQUEST" -slug="$VCS_SLUG" -tag="$VCS_TAG" -build_url="$CI_BUILD_URL" -build="$CI_BUILD_ID" -job="$CI_JOB_ID" - -beta_xcode_partials="" - -proj_root="$git_root" -gcov_exe="gcov" -gcov_arg="" - -b="\033[0;36m" -g="\033[0;32m" -r="\033[0;31m" -e="\033[0;90m" -x="\033[0m" - -show_help() { -cat << EOF - - Codecov Bash $VERSION - - Global report uploading tool for Codecov - Documentation at https://docs.codecov.io/docs - Contribute at https://github.com/codecov/codecov-bash - - - 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If not present, - the parent will be determined using the API of your repository provider. - When using the repository provider's API, the parent is determined via finding - the closest ancestor to the commit. - - -R root dir Used when not in git/hg project to identify project root directory - -y conf file Used to specify the location of the .codecov.yml config file - -F flag Flag the upload to group coverage metrics - - -F unittests This upload is only unittests - -F integration This upload is only integration tests - -F ui,chrome This upload is Chrome - UI tests - - -c Move discovered coverage reports to the trash - -Z Exit with 1 if not successful. Default will Exit with 0 - - -- xcode -- - -D Custom Derived Data Path for Coverage.profdata and gcov processing - Default '~/Library/Developer/Xcode/DerivedData' - -J Specify packages to build coverage. - This can significantly reduces time to build coverage reports. - - -J 'MyAppName' Will match "MyAppName" and "MyAppNameTests" - -J '^ExampleApp$' Will match only "ExampleApp" not "ExampleAppTests" - - -- gcov -- - -g GLOB Paths to ignore during gcov gathering - -G GLOB Paths to include during gcov gathering - -p dir Project root directory - Also used when preparing gcov - -k prefix Prefix filepaths to help resolve path fixing: https://github.com/codecov/support/issues/472 - -x gcovexe gcov executable to run. Defaults to 'gcov' - -a gcovargs extra arguments to pass to gcov - - -- Override CI Environment Variables -- - These variables are automatically detected by popular CI providers - - -B branch Specify the branch name - -C sha Specify the commit sha - -P pr Specify the pull request number - -b build Specify the build number - -T tag Specify the git tag - - -- Enterprise -- - -u URL Set the target url for Enterprise customers - Not required when retrieving the bash uploader from your CCE - (option) Set environment variable CODECOV_URL=https://my-hosted-codecov.com - -r SLUG owner/repo slug used instead of the private repo token in Enterprise - (option) set environment variable CODECOV_SLUG=:owner/:repo - (option) set in your codecov.yml "codecov.slug" - -S PATH File path to your cacert.pem file used to verify ssl with Codecov Enterprise (optional) - (option) Set environment variable: CODECOV_CA_BUNDLE="/path/to/ca.pem" - -U curlargs Extra curl arguments to communicate with Codecov. e.g., -U "--proxy http://http-proxy" - -A curlargs Extra curl arguments to communicate with AWS. - - -- Debugging -- - -d Don't upload, but dump upload file to stdout - -K Remove color from the output - -v Verbose mode - -EOF -} - - -say() { - echo -e "$1" -} - - -urlencode() { - echo "$1" | curl -Gso /dev/null -w %{url_effective} --data-urlencode @- "" | cut -c 3- | sed -e 's/%0A//' -} - - -swiftcov() { - _dir=$(dirname "$1" | sed 's/\(Build\).*/\1/g') - for _type in app framework xctest - do - find "$_dir" -name "*.$_type" | while read f - do - _proj=${f##*/} - _proj=${_proj%."$_type"} - if [ "$2" = "" ] || [ "$(echo "$_proj" | grep -i "$2")" != "" ]; - then - say " $g+$x Building reports for $_proj $_type" - dest=$([ -f "$f/$_proj" ] && echo "$f/$_proj" || echo "$f/Contents/MacOS/$_proj") - _proj_name=$(echo "$_proj" | sed -e 's/[[:space:]]//g') - xcrun llvm-cov show $beta_xcode_partials -instr-profile "$1" "$dest" > "$_proj_name.$_type.coverage.txt" \ - || say " ${r}x>${x} llvm-cov failed to produce results for $dest" - fi - done - done -} - - -# Credits to: https://gist.github.com/pkuczynski/8665367 -parse_yaml() { - local prefix=$2 - local s='[[:space:]]*' w='[a-zA-Z0-9_]*' fs=$(echo @|tr @ '\034') - sed -ne "s|^\($s\)\($w\)$s:$s\"\(.*\)\"$s\$|\1$fs\2$fs\3|p" \ - -e "s|^\($s\)\($w\)$s:$s\(.*\)$s\$|\1$fs\2$fs\3|p" $1 | - awk -F$fs '{ - indent = length($1)/2; - vname[indent] = $2; - for (i in vname) {if (i > indent) {delete vname[i]}} - if (length($3) > 0) { - vn=""; if (indent > 0) {vn=(vn)(vname[0])("_")} - printf("%s%s%s=\"%s\"\n", "'$prefix'",vn, $2, $3); - } - }' -} - - -if [ $# != 0 ]; -then - while getopts "a:A:b:B:cC:dD:e:f:F:g:G:hJ:k:Kn:p:P:r:R:y:s:S:t:T:u:U:vx:X:ZN:" o - do - case "$o" in - "N") - parent=$OPTARG - ;; - "a") - gcov_arg=$OPTARG - ;; - "A") - curlawsargs="$OPTARG" - ;; - "b") - build_o="$OPTARG" - ;; - "B") - branch_o="$OPTARG" - ;; - "c") - clean="1" - ;; - "C") - commit_o="$OPTARG" - ;; - "d") - dump="1" - ;; - "D") - ddp="$OPTARG" - ;; - "e") - env="$env,$OPTARG" - ;; - "f") - if [ "${OPTARG::1}" = "!" ]; - then - exclude_cov="$exclude_cov -not -path '${OPTARG:1}'" - - elif [[ "$OPTARG" = *"*"* ]]; - then - include_cov="$include_cov -or -name '$OPTARG'" - - else - ft_search=0 - if [ "$files" = "" ]; - then - files="$OPTARG" - else - files="$files -$OPTARG" - fi - fi - ;; - "F") - if [ "$flags" = "" ]; - then - flags="$OPTARG" - else - flags="$flags,$OPTARG" - fi - ;; - "g") - gcov_ignore="$gcov_ignore -not -path '$OPTARG'" - ;; - "G") - gcov_include="$gcov_include -path '$OPTARG'" - ;; - "h") - show_help - exit 0; - ;; - "J") - ft_xcodellvm="1" - ft_xcodeplist="0" - if [ "$xp" = "" ]; - then - xp="$OPTARG" - else - xp="$xp\|$OPTARG" - fi - ;; - "k") - prefix_o=$(echo "$OPTARG" | sed -e 's:^/*::' -e 's:/*$::') - ;; - "K") - b="" - g="" - r="" - e="" - x="" - ;; - "n") - name="$OPTARG" - ;; - "p") - proj_root="$OPTARG" - ;; - "P") - pr_o="$OPTARG" - ;; - "r") - slug_o="$OPTARG" - ;; - "R") - git_root="$OPTARG" - ;; - "s") - if [ "$search_in_o" = "" ]; - then - search_in_o="$OPTARG" - else - search_in_o="$search_in_o $OPTARG" - fi - ;; - "S") - cacert="--cacert \"$OPTARG\"" - ;; - "t") - if [ "${OPTARG::1}" = "@" ]; - then - token=$(cat "${OPTARG:1}" | tr -d ' \n') - else - token="$OPTARG" - fi - ;; - "T") - tag_o="$OPTARG" - ;; - "u") - url_o=$(echo "$OPTARG" | sed -e 's/\/$//') - ;; - "U") - curlargs="$OPTARG" - ;; - "v") - set -x - curl_s="" - ;; - "x") - gcov_exe=$OPTARG - ;; - "X") - if [ "$OPTARG" = "gcov" ]; - then - ft_gcov="0" - elif [ "$OPTARG" = "coveragepy" ] || [ "$OPTARG" = "py" ]; - then - ft_coveragepy="0" - elif [ "$OPTARG" = "gcovout" ]; - then - ft_gcovout="0" - elif [ "$OPTARG" = "xcodellvm" ]; - then - ft_xcodellvm="1" - ft_xcodeplist="0" - elif [ "$OPTARG" = "fix" ] || [ "$OPTARG" = "fixes" ]; - then - ft_fix="0" - elif [ "$OPTARG" = "xcode" ]; - then - ft_xcodellvm="0" - ft_xcodeplist="0" - elif [ "$OPTARG" = "search" ]; - then - ft_search="0" - elif [ "$OPTARG" = "xcodepartials" ]; - then - beta_xcode_partials="-use-color" - elif [ "$OPTARG" = "network" ]; - then - ft_network="0" - elif [ "$OPTARG" = "s3" ]; - then - ft_s3="0" - fi - ;; - "y") - codecov_yml="$OPTARG" - ;; - "Z") - exit_with=1 - ;; - esac - done -fi - -say " - _____ _ - / ____| | | -| | ___ __| | ___ ___ _____ __ -| | / _ \\ / _\` |/ _ \\/ __/ _ \\ \\ / / -| |___| (_) | (_| | __/ (_| (_) \\ V / - \\_____\\___/ \\__,_|\\___|\\___\\___/ \\_/ - Bash-$VERSION - -" - -search_in="$proj_root" - -if [ "$JENKINS_URL" != "" ]; -then - say "$e==>$x Jenkins CI detected." - # https://wiki.jenkins-ci.org/display/JENKINS/Building+a+software+project - # https://wiki.jenkins-ci.org/display/JENKINS/GitHub+pull+request+builder+plugin#GitHubpullrequestbuilderplugin-EnvironmentVariables - service="jenkins" - - if [ "$ghprbSourceBranch" != "" ]; - then - branch="$ghprbSourceBranch" - elif [ "$GIT_BRANCH" != "" ]; - then - branch="$GIT_BRANCH" - elif [ "$BRANCH_NAME" != "" ]; - then - branch="$BRANCH_NAME" - fi - - if [ "$ghprbActualCommit" != "" ]; - then - commit="$ghprbActualCommit" - elif [ "$GIT_COMMIT" != "" ]; - then - commit="$GIT_COMMIT" - fi - - if [ "$ghprbPullId" != "" ]; - then - pr="$ghprbPullId" - elif [ "$CHANGE_ID" != "" ]; - then - pr="$CHANGE_ID" - fi - - build="$BUILD_NUMBER" - build_url=$(urlencode "$BUILD_URL") - -elif [ "$CI" = "true" ] && [ "$TRAVIS" = "true" ] && [ "$SHIPPABLE" != "true" ]; -then - say "$e==>$x Travis CI detected." - # https://docs.travis-ci.com/user/environment-variables/ - service="travis" - commit="${TRAVIS_PULL_REQUEST_SHA:-$TRAVIS_COMMIT}" - build="$TRAVIS_JOB_NUMBER" - pr="$TRAVIS_PULL_REQUEST" - job="$TRAVIS_JOB_ID" - slug="$TRAVIS_REPO_SLUG" - env="$env,TRAVIS_OS_NAME" - tag="$TRAVIS_TAG" - if [ "$TRAVIS_BRANCH" != "$TRAVIS_TAG" ]; - then - branch="$TRAVIS_BRANCH" - fi - - language=$(compgen -A variable | grep "^TRAVIS_.*_VERSION$" | head -1) - if [ "$language" != "" ]; - then - env="$env,${!language}" - fi - -elif [ "$CODEBUILD_BUILD_ARN" != "" ]; -then - say "$e==>$x AWS Codebuild detected." - # https://docs.aws.amazon.com/codebuild/latest/userguide/build-env-ref-env-vars.html - service="codebuild" - commit="$CODEBUILD_RESOLVED_SOURCE_VERSION" - build="$CODEBUILD_BUILD_ID" - branch="$(echo $CODEBUILD_WEBHOOK_HEAD_REF | sed 's/^refs\/heads\///')" - if [ "${CODEBUILD_SOURCE_VERSION/pr}" = "$CODEBUILD_SOURCE_VERSION" ] ; then - pr="false" - else - pr="$(echo $CODEBUILD_SOURCE_VERSION | sed 's/^pr\///')" - fi - job="$CODEBUILD_BUILD_ID" - slug="$(echo $CODEBUILD_SOURCE_REPO_URL | sed 's/^.*github.com\///' | sed 's/\.git$//')" - -elif [ "$DOCKER_REPO" != "" ]; -then - say "$e==>$x Docker detected." - # https://docs.docker.com/docker-cloud/builds/advanced/ - service="docker" - branch="$SOURCE_BRANCH" - commit="$SOURCE_COMMIT" - slug="$DOCKER_REPO" - tag="$CACHE_TAG" - env="$env,IMAGE_NAME" - -elif [ "$CI" = "true" ] && [ "$CI_NAME" = "codeship" ]; -then - say "$e==>$x Codeship CI detected." - # https://www.codeship.io/documentation/continuous-integration/set-environment-variables/ - service="codeship" - branch="$CI_BRANCH" - build="$CI_BUILD_NUMBER" - build_url=$(urlencode "$CI_BUILD_URL") - commit="$CI_COMMIT_ID" - -elif [ ! -z "$CF_BUILD_URL" ] && [ ! -z "$CF_BUILD_ID" ]; -then - say "$e==>$x Codefresh CI detected." - # https://docs.codefresh.io/v1.0/docs/variables - service="codefresh" - branch="$CF_BRANCH" - build="$CF_BUILD_ID" - build_url=$(urlencode "$CF_BUILD_URL") - commit="$CF_REVISION" - -elif [ "$TEAMCITY_VERSION" != "" ]; -then - say "$e==>$x TeamCity CI detected." - # https://confluence.jetbrains.com/display/TCD8/Predefined+Build+Parameters - # https://confluence.jetbrains.com/plugins/servlet/mobile#content/view/74847298 - if [ "$TEAMCITY_BUILD_BRANCH" = '' ]; - then - echo " Teamcity does not automatically make build parameters available as environment variables." - echo " Add the following environment parameters to the build configuration" - echo " env.TEAMCITY_BUILD_BRANCH = %teamcity.build.branch%" - echo " env.TEAMCITY_BUILD_ID = %teamcity.build.id%" - echo " env.TEAMCITY_BUILD_URL = %teamcity.serverUrl%/viewLog.html?buildId=%teamcity.build.id%" - echo " env.TEAMCITY_BUILD_COMMIT = %system.build.vcs.number%" - echo " env.TEAMCITY_BUILD_REPOSITORY = %vcsroot..url%" - fi - service="teamcity" - branch="$TEAMCITY_BUILD_BRANCH" - build="$TEAMCITY_BUILD_ID" - build_url=$(urlencode "$TEAMCITY_BUILD_URL") - if [ "$TEAMCITY_BUILD_COMMIT" != "" ]; - then - commit="$TEAMCITY_BUILD_COMMIT" - else - commit="$BUILD_VCS_NUMBER" - fi - remote_addr="$TEAMCITY_BUILD_REPOSITORY" - -elif [ "$CI" = "true" ] && [ "$CIRCLECI" = "true" ]; -then - say "$e==>$x Circle CI detected." - # https://circleci.com/docs/environment-variables - service="circleci" - branch="$CIRCLE_BRANCH" - build="$CIRCLE_BUILD_NUM" - job="$CIRCLE_NODE_INDEX" - if [ "$CIRCLE_PROJECT_REPONAME" != "" ]; - then - slug="$CIRCLE_PROJECT_USERNAME/$CIRCLE_PROJECT_REPONAME" - else - # git@github.com:owner/repo.git - slug="${CIRCLE_REPOSITORY_URL##*:}" - # owner/repo.git - slug="${slug%%.git}" - fi - pr="$CIRCLE_PR_NUMBER" - commit="$CIRCLE_SHA1" - search_in="$search_in $CIRCLE_ARTIFACTS $CIRCLE_TEST_REPORTS" - -elif [ "$BUDDYBUILD_BRANCH" != "" ]; -then - say "$e==>$x buddybuild detected" - # http://docs.buddybuild.com/v6/docs/custom-prebuild-and-postbuild-steps - service="buddybuild" - branch="$BUDDYBUILD_BRANCH" - build="$BUDDYBUILD_BUILD_NUMBER" - build_url="https://dashboard.buddybuild.com/public/apps/$BUDDYBUILD_APP_ID/build/$BUDDYBUILD_BUILD_ID" - # BUDDYBUILD_TRIGGERED_BY - if [ "$ddp" = "$(echo ~)/Library/Developer/Xcode/DerivedData" ]; - then - ddp="/private/tmp/sandbox/${BUDDYBUILD_APP_ID}/bbtest" - fi - -elif [ "${bamboo_planRepository_revision}" != "" ]; -then - say "$e==>$x Bamboo detected" - # https://confluence.atlassian.com/bamboo/bamboo-variables-289277087.html#Bamboovariables-Build-specificvariables - service="bamboo" - commit="${bamboo_planRepository_revision}" - branch="${bamboo_planRepository_branch}" - build="${bamboo_buildNumber}" - build_url="${bamboo_buildResultsUrl}" - remote_addr="${bamboo_planRepository_repositoryUrl}" - -elif [ "$CI" = "true" ] && [ "$BITRISE_IO" = "true" ]; -then - # http://devcenter.bitrise.io/faq/available-environment-variables/ - say "$e==>$x Bitrise CI detected." - service="bitrise" - branch="$BITRISE_GIT_BRANCH" - build="$BITRISE_BUILD_NUMBER" - build_url=$(urlencode "$BITRISE_BUILD_URL") - pr="$BITRISE_PULL_REQUEST" - if [ "$GIT_CLONE_COMMIT_HASH" != "" ]; - then - commit="$GIT_CLONE_COMMIT_HASH" - fi - -elif [ "$CI" = "true" ] && [ "$SEMAPHORE" = "true" ]; -then - say "$e==>$x Semaphore CI detected." - # https://semaphoreapp.com/docs/available-environment-variables.html - service="semaphore" - branch="$BRANCH_NAME" - build="$SEMAPHORE_BUILD_NUMBER" - job="$SEMAPHORE_CURRENT_THREAD" - pr="$PULL_REQUEST_NUMBER" - slug="$SEMAPHORE_REPO_SLUG" - commit="$REVISION" - env="$env,SEMAPHORE_TRIGGER_SOURCE" - -elif [ "$CI" = "true" ] && [ "$BUILDKITE" = "true" ]; -then - say "$e==>$x Buildkite CI detected." - # https://buildkite.com/docs/guides/environment-variables - service="buildkite" - branch="$BUILDKITE_BRANCH" - build="$BUILDKITE_BUILD_NUMBER" - job="$BUILDKITE_JOB_ID" - build_url=$(urlencode "$BUILDKITE_BUILD_URL") - slug="$BUILDKITE_PROJECT_SLUG" - commit="$BUILDKITE_COMMIT" - if [[ "$BUILDKITE_PULL_REQUEST" != "false" ]]; then - pr="$BUILDKITE_PULL_REQUEST" - fi - tag="$BUILDKITE_TAG" - -elif [ "$CI" = "drone" ] || [ "$DRONE" = "true" ]; -then - say "$e==>$x Drone CI detected." - # http://docs.drone.io/env.html - # drone commits are not full shas - service="drone.io" - branch="$DRONE_BRANCH" - build="$DRONE_BUILD_NUMBER" - build_url=$(urlencode "${DRONE_BUILD_LINK}") - pr="$DRONE_PULL_REQUEST" - job="$DRONE_JOB_NUMBER" - tag="$DRONE_TAG" - -elif [ "$HEROKU_TEST_RUN_BRANCH" != "" ]; -then - say "$e==>$x Heroku CI detected." - # https://devcenter.heroku.com/articles/heroku-ci#environment-variables - service="heroku" - branch="$HEROKU_TEST_RUN_BRANCH" - build="$HEROKU_TEST_RUN_ID" - -elif [ "$CI" = "True" ] && [ "$APPVEYOR" = "True" ]; -then - say "$e==>$x Appveyor CI detected." - # http://www.appveyor.com/docs/environment-variables - service="appveyor" - branch="$APPVEYOR_REPO_BRANCH" - build=$(urlencode "$APPVEYOR_JOB_ID") - pr="$APPVEYOR_PULL_REQUEST_NUMBER" - job="$APPVEYOR_ACCOUNT_NAME%2F$APPVEYOR_PROJECT_SLUG%2F$APPVEYOR_BUILD_VERSION" - slug="$APPVEYOR_REPO_NAME" - commit="$APPVEYOR_REPO_COMMIT" - build_url=$(urlencode "${APPVEYOR_URL}/project/${APPVEYOR_REPO_NAME}/builds/$APPVEYOR_BUILD_ID/job/${APPVEYOR_JOB_ID}") -elif [ "$CI" = "true" ] && [ "$WERCKER_GIT_BRANCH" != "" ]; -then - say "$e==>$x Wercker CI detected." - # http://devcenter.wercker.com/articles/steps/variables.html - service="wercker" - branch="$WERCKER_GIT_BRANCH" - build="$WERCKER_MAIN_PIPELINE_STARTED" - slug="$WERCKER_GIT_OWNER/$WERCKER_GIT_REPOSITORY" - commit="$WERCKER_GIT_COMMIT" - -elif [ "$CI" = "true" ] && [ "$MAGNUM" = "true" ]; -then - say "$e==>$x Magnum CI detected." - # https://magnum-ci.com/docs/environment - service="magnum" - branch="$CI_BRANCH" - build="$CI_BUILD_NUMBER" - commit="$CI_COMMIT" - -elif [ "$SHIPPABLE" = "true" ]; -then - say "$e==>$x Shippable CI detected." - # http://docs.shippable.com/ci_configure/ - service="shippable" - branch=$([ "$HEAD_BRANCH" != "" ] && echo "$HEAD_BRANCH" || echo "$BRANCH") - build="$BUILD_NUMBER" - build_url=$(urlencode "$BUILD_URL") - pr="$PULL_REQUEST" - slug="$REPO_FULL_NAME" - commit="$COMMIT" - -elif [ "$TDDIUM" = "true" ]; -then - say "Solano CI detected." - # http://docs.solanolabs.com/Setup/tddium-set-environment-variables/ - service="solano" - commit="$TDDIUM_CURRENT_COMMIT" - branch="$TDDIUM_CURRENT_BRANCH" - build="$TDDIUM_TID" - pr="$TDDIUM_PR_ID" - -elif [ "$GREENHOUSE" = "true" ]; -then - say "$e==>$x Greenhouse CI detected." - # http://docs.greenhouseci.com/docs/environment-variables-files - service="greenhouse" - branch="$GREENHOUSE_BRANCH" - build="$GREENHOUSE_BUILD_NUMBER" - build_url=$(urlencode "$GREENHOUSE_BUILD_URL") - pr="$GREENHOUSE_PULL_REQUEST" - commit="$GREENHOUSE_COMMIT" - search_in="$search_in $GREENHOUSE_EXPORT_DIR" - -elif [ "$GITLAB_CI" != "" ]; -then - say "$e==>$x GitLab CI detected." - # http://doc.gitlab.com/ce/ci/variables/README.html - service="gitlab" - branch="${CI_BUILD_REF_NAME:-$CI_COMMIT_REF_NAME}" - build="${CI_BUILD_ID:-$CI_JOB_ID}" - remote_addr="${CI_BUILD_REPO:-$CI_REPOSITORY_URL}" - commit="${CI_BUILD_REF:-$CI_COMMIT_SHA}" - slug="${CI_PROJECT_PATH}" - -elif [ "$GITHUB_ACTION" != "" ]; -then - say "$e==>$x GitHub Actions detected." - - # https://github.com/features/actions - service="github-actions" - - # https://help.github.com/en/articles/virtual-environments-for-github-actions#environment-variables - branch="${GITHUB_REF#refs/heads/}" - commit="${GITHUB_SHA}" - slug="${GITHUB_REPOSITORY}" - -elif [ "$SYSTEM_TEAMFOUNDATIONSERVERURI" != "" ]; -then - say "$e==>$x Azure Pipelines detected." - # https://docs.microsoft.com/en-us/azure/devops/pipelines/build/variables?view=vsts - service="azure_pipelines" - commit="$BUILD_SOURCEVERSION" - build="$BUILD_BUILDNUMBER" - if [ -z "$PULL_REQUEST_NUMBER" ]; - then - pr="$PULL_REQUEST_ID" - else - pr="$PULL_REQUEST_NUMBER" - fi - project="${SYSTEM_TEAMPROJECT}" - server_uri="${SYSTEM_TEAMFOUNDATIONSERVERURI}" - job="${BUILD_BUILDID}" - branch="$BUILD_SOURCEBRANCHNAME" - build_url=$(urlencode "${SYSTEM_TEAMFOUNDATIONSERVERURI}${SYSTEM_TEAMPROJECT}/_build/results?buildId=${BUILD_BUILDID}") -elif [ "$CI" = "true" ] && [ "$BITBUCKET_BUILD_NUMBER" != "" ]; -then - say "$e==>$x Bitbucket detected." - # https://confluence.atlassian.com/bitbucket/variables-in-pipelines-794502608.html - service="bitbucket" - branch="$BITBUCKET_BRANCH" - build="$BITBUCKET_BUILD_NUMBER" - slug="$BITBUCKET_REPO_OWNER/$BITBUCKET_REPO_SLUG" - job="$BITBUCKET_BUILD_NUMBER" - pr="$BITBUCKET_PR_ID" - commit="$BITBUCKET_COMMIT" - # See https://jira.atlassian.com/browse/BCLOUD-19393 - if [ "${#commit}" = 12 ]; - then - commit=$(git rev-parse "$BITBUCKET_COMMIT") - fi -elif [ "$CIRRUS_CI" != "" ]; -then - say "$e==>$x Cirrus CI detected." - # https://cirrus-ci.org/guide/writing-tasks/#environment-variables - service="cirrus-ci" - slug="$CIRRUS_REPO_FULL_NAME" - branch="$CIRRUS_BRANCH" - pr="$CIRRUS_PR" - commit="$CIRRUS_CHANGE_IN_REPO" - build="$CIRRUS_TASK_ID" - job="$CIRRUS_TASK_NAME" -else - say "${r}x>${x} No CI provider detected." - say " Testing inside Docker? ${b}http://docs.codecov.io/docs/testing-with-docker${x}" - say " Testing with Tox? ${b}https://docs.codecov.io/docs/python#section-testing-with-tox${x}" - -fi - -say " ${e}project root:${x} $git_root" - -# find branch, commit, repo from git command -if [ "$GIT_BRANCH" != "" ]; -then - branch="$GIT_BRANCH" - -elif [ "$branch" = "" ]; -then - branch=$(git rev-parse --abbrev-ref HEAD 2>/dev/null || hg branch 2>/dev/null || echo "") - if [ "$branch" = "HEAD" ]; - then - branch="" - fi -fi - -if [ "$commit_o" = "" ]; -then - # merge commit -> actual commit - mc= - if [ -n "$pr" ] && [ "$pr" != false ]; - then - mc=$(git show --no-patch --format="%P" 2>/dev/null || echo "") - fi - if [[ "$mc" =~ ^[a-z0-9]{40}[[:space:]][a-z0-9]{40}$ ]]; - then - say " Fixing merge commit SHA" - commit=$(echo "$mc" | cut -d' ' -f2) - elif [ "$GIT_COMMIT" != "" ]; - then - commit="$GIT_COMMIT" - elif [ "$commit" = "" ]; - then - commit=$(git log -1 --format="%H" 2>/dev/null || hg id -i --debug 2>/dev/null | tr -d '+' || echo "") - fi -else - commit="$commit_o" -fi - -if [ "$CODECOV_TOKEN" != "" ] && [ "$token" = "" ]; -then - say "${e}-->${x} token set from env" - token="$CODECOV_TOKEN" -fi - -if [ "$CODECOV_URL" != "" ] && [ "$url_o" = "" ]; -then - say "${e}-->${x} url set from env" - url_o=$(echo "$CODECOV_URL" | sed -e 's/\/$//') -fi - -if [ "$CODECOV_SLUG" != "" ]; -then - say "${e}-->${x} slug set from env" - slug_o="$CODECOV_SLUG" - -elif [ "$slug" = "" ]; -then - if [ "$remote_addr" = "" ]; - then - remote_addr=$(git config --get remote.origin.url || hg paths default || echo '') - fi - if [ "$remote_addr" != "" ]; - then - if echo "$remote_addr" | grep -q "//"; then - # https - slug=$(echo "$remote_addr" | cut -d / -f 4,5 | sed -e 's/\.git$//') - else - # ssh - slug=$(echo "$remote_addr" | cut -d : -f 2 | sed -e 's/\.git$//') - fi - fi - if [ "$slug" = "/" ]; - then - slug="" - fi -fi - -yaml=$(test -n "$codecov_yml" && echo "$codecov_yml" \ - || cd "$git_root" && \ - git ls-files "*codecov.yml" "*codecov.yaml" 2>/dev/null \ - || hg locate "*codecov.yml" "*codecov.yaml" 2>/dev/null \ - || cd $proj_root && find . -type f -name '*codecov.y*ml' -depth 1 2>/dev/null \ - || echo '') -yaml=$(echo "$yaml" | head -1) - -if [ "$yaml" != "" ]; -then - say " ${e}Yaml found at:${x} $yaml" - config=$(parse_yaml "$git_root/$yaml" || echo '') - - # TODO validate the yaml here - - if [ "$(echo "$config" | grep 'codecov_token="')" != "" ] && [ "$token" = "" ]; - then - say "${e}-->${x} token set from yaml" - token="$(echo "$config" | grep 'codecov_token="' | sed -e 's/codecov_token="//' | sed -e 's/"\.*//')" - fi - - if [ "$(echo "$config" | grep 'codecov_url="')" != "" ] && [ "$url_o" = "" ]; - then - say "${e}-->${x} url set from yaml" - url_o="$(echo "$config" | grep 'codecov_url="' | sed -e 's/codecov_url="//' | sed -e 's/"\.*//')" - fi - - if [ "$(echo "$config" | grep 'codecov_slug="')" != "" ] && [ "$slug_o" = "" ]; - then - say "${e}-->${x} slug set from yaml" - slug_o="$(echo "$config" | grep 'codecov_slug="' | sed -e 's/codecov_slug="//' | sed -e 's/"\.*//')" - fi -else - say " ${g}Yaml not found, that's ok! Learn more at${x} ${b}http://docs.codecov.io/docs/codecov-yaml${x}" - -fi - -if [ "$branch_o" != "" ]; -then - branch=$(urlencode "$branch_o") -else - branch=$(urlencode "$branch") -fi - -query="branch=$branch\ - &commit=$commit\ - &build=$([ "$build_o" = "" ] && echo "$build" || echo "$build_o")\ - &build_url=$build_url\ - &name=$(urlencode "$name")\ - &tag=$([ "$tag_o" = "" ] && echo "$tag" || echo "$tag_o")\ - &slug=$([ "$slug_o" = "" ] && urlencode "$slug" || urlencode "$slug_o")\ - &service=$service\ - &flags=$flags\ - &pr=$([ "$pr_o" = "" ] && echo "${pr##\#}" || echo "${pr_o##\#}")\ - &job=$job" - -if [ ! -z "$project" ] && [ ! -z "$server_uri" ]; -then - query=$(echo "$query&project=$project&server_uri=$server_uri" | tr -d ' ') -fi - -if [ "$parent" != "" ]; -then - query=$(echo "parent=$parent&$query" | tr -d ' ') -fi - -if [ "$ft_search" = "1" ]; -then - # detect bower comoponents location - bower_components="bower_components" - bower_rc=$(cd "$git_root" && cat .bowerrc 2>/dev/null || echo "") - if [ "$bower_rc" != "" ]; - then - bower_components=$(echo "$bower_rc" | tr -d '\n' | grep '"directory"' | cut -d'"' -f4 | sed -e 's/\/$//') - if [ "$bower_components" = "" ]; - then - bower_components="bower_components" - fi - fi - - # Swift Coverage - if [ "$ft_xcodellvm" = "1" ] && [ -d "$ddp" ]; - then - say "${e}==>${x} Processing Xcode reports via llvm-cov" - say " DerivedData folder: $ddp" - profdata_files=$(find "$ddp" -name '*.profdata' 2>/dev/null || echo '') - if [ "$profdata_files" != "" ]; - then - # xcode via profdata - if [ "$xp" = "" ]; - then - # xp=$(xcodebuild -showBuildSettings 2>/dev/null | grep -i "^\s*PRODUCT_NAME" | sed -e 's/.*= \(.*\)/\1/') - # say " ${e}->${x} Speed up Xcode processing by adding ${e}-J '$xp'${x}" - say " ${g}hint${x} Speed up Swift processing by using use ${g}-J 'AppName'${x} (regexp accepted)" - say " ${g}hint${x} This will remove Pods/ from your report. Also ${b}https://docs.codecov.io/docs/ignoring-paths${x}" - fi - while read -r profdata; - do - if [ "$profdata" != "" ]; - then - swiftcov "$profdata" "$xp" - fi - done <<< "$profdata_files" - else - say " ${e}->${x} No Swift coverage found" - fi - - # Obj-C Gcov Coverage - if [ "$ft_gcov" = "1" ]; - then - say " ${e}->${x} Running $gcov_exe for Obj-C" - if [ "$ft_gcovout" = "1" ]; - then - # suppress gcov output - bash -c "find $ddp -type f -name '*.gcda' $gcov_include $gcov_ignore -exec $gcov_exe -p $gcov_arg {} +" || true 2>/dev/null - else - bash -c "find $ddp -type f -name '*.gcda' $gcov_include $gcov_ignore -exec $gcov_exe -p $gcov_arg {} +" || true - fi - fi - fi - - if [ "$ft_xcodeplist" = "1" ] && [ -d "$ddp" ]; - then - say "${e}==>${x} Processing Xcode plists" - plists_files=$(find "$ddp" -name '*.xccoverage' 2>/dev/null || echo '') - if [ "$plists_files" != "" ]; - then - while read -r plist; - do - if [ "$plist" != "" ]; - then - say " ${g}Found${x} plist file at $plist" - plutil -convert xml1 -o "$(basename "$plist").plist" -- $plist - fi - done <<< "$plists_files" - fi - fi - - # Gcov Coverage - if [ "$ft_gcov" = "1" ]; - then - say "${e}==>${x} Running gcov in $proj_root ${e}(disable via -X gcov)${x}" - bash -c "find $proj_root -type f -name '*.gcno' $gcov_include $gcov_ignore -execdir $gcov_exe -pb $gcov_arg {} +" || true - else - say "${e}==>${x} gcov disabled" - fi - - # Python Coverage - if [ "$ft_coveragepy" = "1" ]; - then - if [ ! -f coverage.xml ]; - then - if which coverage >/dev/null 2>&1; - then - say "${e}==>${x} Python coveragepy exists ${e}disable via -X coveragepy${x}" - - dotcoverage=$(find "$git_root" -name '.coverage' -or -name '.coverage.*' | head -1 || echo '') - if [ "$dotcoverage" != "" ]; - then - cd "$(dirname "$dotcoverage")" - if [ ! -f .coverage ]; - then - say " ${e}->${x} Running coverage combine" - coverage combine -a - fi - say " ${e}->${x} Running coverage xml" - if [ "$(coverage xml -i)" != "No data to report." ]; - then - files="$files -$PWD/coverage.xml" - else - say " ${r}No data to report.${x}" - fi - cd "$proj_root" - else - say " ${r}No .coverage file found.${x}" - fi - else - say "${e}==>${x} Python coveragepy not found" - fi - fi - else - say "${e}==>${x} Python coveragepy disabled" - fi - - if [ "$search_in_o" != "" ]; - then - # location override - search_in="$search_in_o" - fi - - say "$e==>$x Searching for coverage reports in:" - for _path in $search_in - do - say " ${g}+${x} $_path" - done - - patterns="find $search_in \( \ - -name vendor \ - -or -name htmlcov \ - -or -name virtualenv \ - -or -name js/generated/coverage \ - -or -name .virtualenv \ - -or -name virtualenvs \ - -or -name .virtualenvs \ - -or -name .env \ - -or -name .envs \ - -or -name env \ - -or -name .yarn-cache \ - -or -name envs \ - -or -name .venv \ - -or -name .venvs \ - -or -name venv \ - -or -name venvs \ - -or -name .git \ - -or -name .hg \ - -or -name .tox \ - -or -name __pycache__ \ - -or -name '.egg-info*' \ - -or -name '$bower_components' \ - -or -name node_modules \ - -or -name 'conftest_*.c.gcov' \ - \) -prune -or \ - -type f \( -name '*coverage*.*' \ - -or -name 'nosetests.xml' \ - -or -name 'jacoco*.xml' \ - -or -name 'clover.xml' \ - -or -name 'report.xml' \ - -or -name '*.codecov.*' \ - -or -name 'codecov.*' \ - -or -name 'cobertura.xml' \ - -or -name 'excoveralls.json' \ - -or -name 'luacov.report.out' \ - -or -name 'coverage-final.json' \ - -or -name 'naxsi.info' \ - -or -name 'lcov.info' \ - -or -name 'lcov.dat' \ - -or -name '*.lcov' \ - -or -name '*.clover' \ - -or -name 'cover.out' \ - -or -name 'gcov.info' \ - -or -name '*.gcov' \ - -or -name '*.lst' \ - $include_cov \) \ - $exclude_cov \ - -not -name '*.profdata' \ - -not -name 'coverage-summary.json' \ - -not -name 'phpunit-code-coverage.xml' \ - -not -name '*/classycle/report.xml' \ - -not -name 'remapInstanbul.coverage*.json' \ - -not -name 'phpunit-coverage.xml' \ - -not -name '*codecov.yml' \ - -not -name '*.serialized' \ - -not -name '.coverage*' \ - -not -name '.*coveragerc' \ - -not -name '*.sh' \ - -not -name '*.bat' \ - -not -name '*.ps1' \ - -not -name '*.env' \ - -not -name '*.cmake' \ - -not -name '*.dox' \ - -not -name '*.ec' \ - -not -name '*.rst' \ - -not -name '*.h' \ - -not -name '*.scss' \ - -not -name '*.o' \ - -not -name '*.proto' \ - -not -name '*.sbt' \ - -not -name '*.xcoverage.*' \ - -not -name '*.gz' \ - -not -name '*.conf' \ - -not -name '*.p12' \ - -not -name '*.csv' \ - -not -name '*.rsp' \ - -not -name '*.m4' \ - -not -name '*.pem' \ - -not -name '*~' \ - -not -name '*.exe' \ - -not -name '*.am' \ - -not -name '*.template' \ - -not -name '*.cp' \ - -not -name '*.bw' \ - -not -name '*.crt' \ - -not -name '*.log' \ - -not -name '*.cmake' \ - -not -name '*.pth' \ - -not -name '*.in' \ - -not -name '*.jar*' \ - -not -name '*.pom*' \ - -not -name '*.png' \ - -not -name '*.jpg' \ - -not -name '*.sql' \ - -not -name '*.jpeg' \ - -not -name '*.svg' \ - -not -name '*.gif' \ - -not -name '*.csv' \ - -not -name '*.snapshot' \ - -not -name '*.mak*' \ - -not -name '*.bash' \ - -not -name '*.data' \ - -not -name '*.py' \ - -not -name '*.class' \ - -not -name '*.xcconfig' \ - -not -name '*.ec' \ - -not -name '*.coverage' \ - -not -name '*.pyc' \ - -not -name '*.cfg' \ - -not -name '*.egg' \ - -not -name '*.ru' \ - -not -name '*.css' \ - -not -name '*.less' \ - -not -name '*.pyo' \ - -not -name '*.whl' \ - -not -name '*.html' \ - -not -name '*.ftl' \ - -not -name '*.erb' \ - -not -name '*.rb' \ - -not -name '*.js' \ - -not -name '*.jade' \ - -not -name '*.db' \ - -not -name '*.md' \ - -not -name '*.cpp' \ - -not -name '*.gradle' \ - -not -name '*.tar.tz' \ - -not -name '*.scss' \ - -not -name 'include.lst' \ - -not -name 'fullLocaleNames.lst' \ - -not -name 'inputFiles.lst' \ - -not -name 'createdFiles.lst' \ - -not -name 'scoverage.measurements.*' \ - -not -name 'test_*_coverage.txt' \ - -not -name 'testrunner-coverage*' \ - -print 2>/dev/null" - files=$(eval "$patterns" || echo '') - -elif [ "$include_cov" != "" ]; -then - files=$(eval "find $search_in -type f \( ${include_cov:5} \)$exclude_cov 2>/dev/null" || echo '') -fi - -num_of_files=$(echo "$files" | wc -l | tr -d ' ') -if [ "$num_of_files" != '' ] && [ "$files" != '' ]; -then - say " ${e}->${x} Found $num_of_files reports" -fi - -# no files found -if [ "$files" = "" ]; -then - say "${r}-->${x} No coverage report found." - say " Please visit ${b}http://docs.codecov.io/docs/supported-languages${x}" - exit ${exit_with}; -fi - -if [ "$ft_network" == "1" ]; -then - say "${e}==>${x} Detecting git/mercurial file structure" - network=$(cd "$git_root" && git ls-files 2>/dev/null || hg locate 2>/dev/null || echo "") - if [ "$network" = "" ]; - then - network=$(find "$git_root" \( \ - -name virtualenv \ - -name .virtualenv \ - -name virtualenvs \ - -name .virtualenvs \ - -name '*.png' \ - -name '*.gif' \ - -name '*.jpg' \ - -name '*.jpeg' \ - -name '*.md' \ - -name .env \ - -name .envs \ - -name env \ - -name envs \ - -name .venv \ - -name .venvs \ - -name venv \ - -name venvs \ - -name .git \ - -name .egg-info \ - -name shunit2-2.1.6 \ - -name vendor \ - -name __pycache__ \ - -name node_modules \ - -path '*/$bower_components/*' \ - -path '*/target/delombok/*' \ - -path '*/build/lib/*' \ - -path '*/js/generated/coverage/*' \ - \) -prune -or \ - -type f -print 2>/dev/null || echo '') - fi - - if [ "$prefix_o" != "" ]; - then - network=$(echo "$network" | awk "{print \"$prefix_o/\"\$0}") - fi -fi - -upload_file=`mktemp /tmp/codecov.XXXXXX` -adjustments_file=`mktemp /tmp/codecov.adjustments.XXXXXX` - -cleanup() { - rm -f $upload_file $adjustments_file $upload_file.gz -} - -trap cleanup INT ABRT TERM - -if [ "$env" != "" ]; -then - inc_env="" - say "${e}==>${x} Appending build variables" - for varname in $(echo "$env" | tr ',' ' ') - do - if [ "$varname" != "" ]; - then - say " ${g}+${x} $varname" - inc_env="${inc_env}${varname}=$(eval echo "\$${varname}") -" - fi - done - -echo "$inc_env<<<<<< ENV" >> $upload_file -fi - -# Append git file list -# write discovered yaml location -echo "$yaml" >> $upload_file -if [ "$ft_network" == "1" ]; -then - i="woff|eot|otf" # fonts - i="$i|gif|png|jpg|jpeg|psd" # images - i="$i|ptt|pptx|numbers|pages|md|txt|xlsx|docx|doc|pdf|html|csv" # docs - i="$i|yml|yaml|.gitignore" # supporting docs - echo "$network" | grep -vwE "($i)$" >> $upload_file -fi -echo "<<<<<< network" >> $upload_file - -fr=0 -say "${e}==>${x} Reading reports" -while IFS='' read -r file; -do - # read the coverage file - if [ "$(echo "$file" | tr -d ' ')" != '' ]; - then - if [ -f "$file" ]; - then - report_len=$(wc -c < "$file") - if [ "$report_len" -ne 0 ]; - then - say " ${g}+${x} $file ${e}bytes=$(echo "$report_len" | tr -d ' ')${x}" - # append to to upload - _filename=$(basename "$file") - if [ "${_filename##*.}" = 'gcov' ]; - then - echo "# path=$(echo "$file.reduced" | sed "s|^$git_root/||")" >> $upload_file - # get file name - head -1 $file >> $upload_file - # 1. remove source code - # 2. remove ending bracket lines - # 3. remove whitespace - # 4. remove contextual lines - # 5. remove function names - awk -F': *' '{print $1":"$2":"}' $file \ - | sed '\/: *} *$/d' \ - | sed 's/^ *//' \ - | sed '/^-/d' \ - | sed 's/^function.*/func/' >> $upload_file - else - echo "# path=$(echo "$file" | sed "s|^$git_root/||")" >> $upload_file - cat "$file" >> $upload_file - fi - echo "<<<<<< EOF" >> $upload_file - fr=1 - if [ "$clean" = "1" ]; - then - rm "$file" - fi - else - say " ${r}-${x} Skipping empty file $file" - fi - else - say " ${r}-${x} file not found at $file" - fi - fi -done <<< "$(echo -e "$files")" - -if [ "$fr" = "0" ]; -then - say "${r}-->${x} No coverage data found." - say " Please visit ${b}http://docs.codecov.io/docs/supported-languages${x}" - say " search for your projects language to learn how to collect reports." - exit ${exit_with}; -fi - -if [ "$ft_fix" = "1" ]; -then - say "${e}==>${x} Appending adjustments" - say " ${b}http://docs.codecov.io/docs/fixing-reports${x}" - - empty_line='^[[:space:]]*$' - # // - syntax_comment='^[[:space:]]*//.*' - # /* or */ - syntax_comment_block='^[[:space:]]*(\/\*|\*\/)[[:space:]]*$' - # { or } - syntax_bracket='^[[:space:]]*[\{\}][[:space:]]*(//.*)?$' - # [ or ] - syntax_list='^[[:space:]]*[][][[:space:]]*(//.*)?$' - - skip_dirs="-not -path '*/$bower_components/*' \ - -not -path '*/node_modules/*'" - - cut_and_join() { - awk 'BEGIN { FS=":" } - $3 ~ /\/\*/ || $3 ~ /\*\// { print $0 ; next } - $1!=key { if (key!="") print out ; key=$1 ; out=$1":"$2 ; next } - { out=out","$2 } - END { print out }' 2>/dev/null - } - - if echo "$network" | grep -m1 '.kt$' 1>/dev/null; - then - # skip brackets and comments - find "$git_root" -type f \ - -name '*.kt' \ - -exec \ - grep -nIHE -e $syntax_bracket \ - -e $syntax_comment_block {} \; \ - | cut_and_join \ - >> $adjustments_file \ - || echo '' - - # last line in file - find "$git_root" -type f \ - -name '*.kt' -exec \ - wc -l {} \; \ - | while read l; do echo "EOF: $l"; done \ - 2>/dev/null \ - >> $adjustments_file \ - || echo '' - - fi - - if echo "$network" | grep -m1 '.go$' 1>/dev/null; - then - # skip empty lines, comments, and brackets - find "$git_root" -not -path '*/vendor/*' \ - -type f \ - -name '*.go' \ - -exec \ - grep -nIHE \ - -e $empty_line \ - -e $syntax_comment \ - -e $syntax_comment_block \ - -e $syntax_bracket \ - {} \; \ - | cut_and_join \ - >> $adjustments_file \ - || echo '' - fi - - if echo "$network" | grep -m1 '.dart$' 1>/dev/null; - then - # skip brackets - find "$git_root" -type f \ - -name '*.dart' \ - -exec \ - grep -nIHE \ - -e $syntax_bracket \ - {} \; \ - | cut_and_join \ - >> $adjustments_file \ - || echo '' - fi - - if echo "$network" | grep -m1 '.php$' 1>/dev/null; - then - # skip empty lines, comments, and brackets - find "$git_root" -not -path "*/vendor/*" \ - -type f \ - -name '*.php' \ - -exec \ - grep -nIHE \ - -e $syntax_list \ - -e $syntax_bracket \ - -e '^[[:space:]]*\);[[:space:]]*(//.*)?$' \ - {} \; \ - | cut_and_join \ - >> $adjustments_file \ - || echo '' - fi - - if echo "$network" | grep -m1 '\(.cpp\|.h\|.cxx\|.c\|.hpp\|.m\)$' 1>/dev/null; - then - # skip brackets - find "$git_root" -type f \ - $skip_dirs \ - \( \ - -name '*.h' \ - -or -name '*.cpp' \ - -or -name '*.cxx' \ - -or -name '*.m' \ - -or -name '*.c' \ - -or -name '*.hpp' \ - \) -exec \ - grep -nIHE \ - -e $empty_line \ - -e $syntax_bracket \ - -e '// LCOV_EXCL' \ - {} \; \ - | cut_and_join \ - >> $adjustments_file \ - || echo '' - - # skip brackets - find "$git_root" -type f \ - $skip_dirs \ - \( \ - -name '*.h' \ - -or -name '*.cpp' \ - -or -name '*.cxx' \ - -or -name '*.m' \ - -or -name '*.c' \ - -or -name '*.hpp' \ - \) -exec \ - grep -nIH '// LCOV_EXCL' \ - {} \; \ - >> $adjustments_file \ - || echo '' - - fi - - found=$(cat $adjustments_file | tr -d ' ') - - if [ "$found" != "" ]; - then - say " ${g}+${x} Found adjustments" - echo "# path=fixes" >> $upload_file - cat $adjustments_file >> $upload_file - echo "<<<<<< EOF" >> $upload_file - rm -rf $adjustments_file - else - say " ${e}->${x} No adjustments found" - fi -fi - -if [ "$url_o" != "" ]; -then - url="$url_o" -fi - -if [ "$dump" != "0" ]; -then - # trim whitespace from query - say " ${e}->${x} Dumping upload file (no upload)" - echo "$url/upload/v4?$(echo "package=bash-$VERSION&token=$token&$query" | tr -d ' ')" - cat $upload_file -else - - say "${e}==>${x} Gzipping contents" - gzip -nf9 $upload_file - - query=$(echo "${query}" | tr -d ' ') - say "${e}==>${x} Uploading reports" - say " ${e}url:${x} $url" - say " ${e}query:${x} $query" - - # Full query without token (to display on terminal output) - queryNoToken=$(echo "package=bash-$VERSION&token=secret&$query" | tr -d ' ') - # now add token to query - query=$(echo "package=bash-$VERSION&token=$token&$query" | tr -d ' ') - - if [ "$ft_s3" = "1" ]; - then - i="0" - while [ $i -lt 4 ] - do - i=$[$i+1] - say " ${e}->${x} Pinging Codecov" - say "$url/upload/v4?$queryNoToken" - res=$(curl $curl_s -X POST $curlargs $cacert \ - -H 'X-Reduced-Redundancy: false' \ - -H 'X-Content-Type: application/x-gzip' \ - "$url/upload/v4?$query" || true) - # a good replay is "https://codecov.io" + "\n" + "https://codecov.s3.amazonaws.com/..." - status=$(echo "$res" | head -1 | grep 'HTTP ' | cut -d' ' -f2) - if [ "$status" = "" ]; - then - s3target=$(echo "$res" | sed -n 2p) - say " ${e}->${x} Uploading" - - - s3=$(curl $curl_s -fiX PUT $curlawsargs \ - --data-binary @$upload_file.gz \ - -H 'Content-Type: application/x-gzip' \ - -H 'Content-Encoding: gzip' \ - -H 'x-amz-acl: public-read' \ - "$s3target" || true) - - - if [ "$s3" != "" ]; - then - say " ${g}->${x} View reports at ${b}$(echo "$res" | sed -n 1p)${x}" - exit 0 - else - say " ${r}X>${x} Failed to upload" - fi - elif [ "$status" = "400" ]; - then - # 400 Error - say "${g}${res}${x}" - exit ${exit_with} - fi - say " ${e}->${x} Sleeping for 30s and trying again..." - sleep 30 - done - fi - - say " ${e}->${x} Uploading to Codecov" - i="0" - while [ $i -lt 4 ] - do - i=$[$i+1] - - res=$(curl $curl_s -X POST $curlargs $cacert \ - --data-binary @$upload_file.gz \ - -H 'Content-Type: text/plain' \ - -H 'Content-Encoding: gzip' \ - -H 'X-Content-Encoding: gzip' \ - -H 'Accept: text/plain' \ - "$url/upload/v2?$query" || echo 'HTTP 500') - # HTTP 200 - # http://.... - status=$(echo "$res" | head -1 | cut -d' ' -f2) - if [ "$status" = "" ]; - then - say " View reports at ${b}$(echo "$res" | head -2 | tail -1)${x}" - exit 0 - - elif [ "${status:0:1}" = "5" ]; - then - say " ${e}->${x} Sleeping for 30s and trying again..." - sleep 30 - - else - say " ${g}${res}${x}" - exit 0 - exit ${exit_with} - fi - - done - - say " ${r}X> Failed to upload coverage reports${x}" -fi - -exit ${exit_with} diff --git a/.github/scripts/filter_tests.sh b/.github/scripts/filter_tests.sh index 88a3dfff5..a01ea5840 100755 --- a/.github/scripts/filter_tests.sh +++ b/.github/scripts/filter_tests.sh @@ -1,9 +1,9 @@ #!/bin/bash -# Exit on failure. set -e git log --format=oneline -n 1 "$GITHUB_SHA" + if [[ $(git log --format=oneline -n 1 "$GITHUB_SHA") = *"noslow"* ]]; then echo "Skipping slow tests..." ./workers/run_tests.sh --exclude-tag=slow "$@" @@ -11,5 +11,3 @@ else echo "Running all tests..." ./workers/run_tests.sh "$@" fi - -./.github/scripts/upload_test_coverage.sh workers diff --git a/.github/scripts/post_deploy_cleanup.sh b/.github/scripts/post_deploy_cleanup.sh index 9901a3b26..a8c1f2fdd 100755 --- a/.github/scripts/post_deploy_cleanup.sh +++ b/.github/scripts/post_deploy_cleanup.sh @@ -1,4 +1,6 @@ -#!/bin/bash -e +#!/bin/bash + +set -e # TODO: this should also be responsible for running another # deploy to remove ci_ingress after the end to end tests run. diff --git a/.github/scripts/upload_test_coverage.sh b/.github/scripts/prepare_test_coverage.sh similarity index 72% rename from .github/scripts/upload_test_coverage.sh rename to .github/scripts/prepare_test_coverage.sh index 12068f485..2ca2510d3 100755 --- a/.github/scripts/upload_test_coverage.sh +++ b/.github/scripts/prepare_test_coverage.sh @@ -1,6 +1,6 @@ #!/bin/bash -# Script to upload code coverage +# Script to prepare code coverage. project=$1 if [[ $project == "" ]]; then @@ -9,7 +9,7 @@ if [[ $project == "" ]]; then fi if [[ $project == "workers" ]]; then - # the workers project uses it's own test_volume directory + # The workers project uses its own test_volume directory. test_volume="workers/test_volume" else test_volume="test_volume" @@ -28,9 +28,3 @@ output_file="${test_volume}/${project}_coverage.xml" # folder. We need to be relative to the repo's root directory. That's why we # append the project folder name to each file path in coverage.xml sed "s/filename=\"/filename=\"$project\//g" "$coverage_file" >"$output_file" - -# codecov.sh is located at https://codecov.io/bash -# we downloaded it for convenience -./.github/scripts/codecov.sh -f "$output_file" -Z -X s3 -F "$project" - -rm -f "$coverage_file" "$output_file" diff --git a/.github/scripts/pull_docker_images.sh b/.github/scripts/pull_docker_images.sh deleted file mode 100755 index 532d0ccab..000000000 --- a/.github/scripts/pull_docker_images.sh +++ /dev/null @@ -1,16 +0,0 @@ -#!/bin/sh - -set -e - -if [ -z "$IMAGES" ]; then - echo "Error: must put images to pull in \$IMAGES" >&2 - exit 1 -fi - -REPO=$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]') - -for image in $IMAGES; do - PACKAGE="$REPO/dr_$image" - # Only pull the package if it already exists. - (docker pull "$PACKAGE" && docker tag "$PACKAGE" "ccdlstaging/dr_$image") || true -done diff --git a/.github/scripts/push_docker_images.sh b/.github/scripts/push_docker_images.sh index ccd79d424..991b54936 100755 --- a/.github/scripts/push_docker_images.sh +++ b/.github/scripts/push_docker_images.sh @@ -7,10 +7,10 @@ if [ -z "$IMAGES" ]; then exit 1 fi -REPO=$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]') +DOCKERHUB_REPO=$(echo "ghcr.io/$GITHUB_REPOSITORY" | tr '[:upper:]' '[:lower:]') for image in $IMAGES; do - PACKAGE="$REPO/dr_$image" + PACKAGE="$DOCKERHUB_REPO/dr_$image" docker tag "ccdlstaging/dr_$image" "$PACKAGE" docker push "$PACKAGE" done diff --git a/.github/scripts/remote_deploy.sh b/.github/scripts/remote_deploy.sh index aabfd0b3b..f93c337c4 100755 --- a/.github/scripts/remote_deploy.sh +++ b/.github/scripts/remote_deploy.sh @@ -26,7 +26,8 @@ run_on_deploy_box() { ssh -o StrictHostKeyChecking=no \ -o ServerAliveInterval=15 \ -i infrastructure/data-refinery-key.pem \ - ubuntu@"${DEPLOY_IP_ADDRESS}" "cd refinebio && $1" + ubuntu@"${DEPLOY_IP_ADDRESS}" \ + "cd refinebio && $1" } # Create file containing local env vars that are needed for deploy. @@ -62,21 +63,21 @@ scp -o StrictHostKeyChecking=no \ -i infrastructure/data-refinery-key.pem \ -r infrastructure/data-refinery-key.pem ubuntu@"$DEPLOY_IP_ADDRESS":refinebio/infrastructure/data-refinery-key.pem -# Output to CircleCI echo "Building new images" # Output to the docker update log. run_on_deploy_box "sudo touch /var/log/docker_update_$CI_TAG.log" run_on_deploy_box "sudo chown ubuntu:ubuntu /var/log/docker_update_$CI_TAG.log" -run_on_deploy_box "source env_vars && echo -e '######\nBuilding new images for $CI_TAG\n######' 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" -run_on_deploy_box "source env_vars && ./.github/scripts/update_docker_image.sh 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" -run_on_deploy_box "source env_vars && echo -e '######\nFinished building new images for $CI_TAG\n######' 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" +run_on_deploy_box ". env_vars && echo -e '######\nBuilding new images for $CI_TAG\n######' 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" +run_on_deploy_box ". env_vars && .github/scripts/update_docker_images.sh 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" +run_on_deploy_box ". env_vars && echo -e '######\nFinished building new images for $CI_TAG\n######' 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" # Load docker_image_exists function and $ALL_IMAGES. +# shellcheck disable=1091 . ./scripts/common.sh -if [[ "$MASTER_OR_DEV" == "master" ]]; then +if [[ "$BRANCH" == "master" ]]; then DOCKERHUB_REPO=ccdl -elif [[ "$MASTER_OR_DEV" == "dev" ]]; then +elif [[ "$BRANCH" == "dev" ]]; then DOCKERHUB_REPO=ccdlstaging else echo "Why in the world was remote_deploy.sh called from a branch other than dev or master?!" @@ -88,10 +89,10 @@ fi # https://github.com/AlexsLemonade/refinebio/issues/784 # Since it's not clear how that happened, the safest thing is to add # an explicit check that the Docker images were successfully updated. -for IMAGE in $ALL_IMAGES; do - image_name="$DOCKERHUB_REPO/dr_$IMAGE" +for image in $ALL_IMAGES; do + image_name="$DOCKERHUB_REPO/dr_$image" if ! docker_image_exists "$image_name" "$CI_TAG"; then - echo "Docker image $image_name:$CI_TAG doesn't exist after running update_docker_image.sh!" + echo "Docker image $image_name:$CI_TAG doesn't exist after running update_docker_images.sh!" echo "This is generally caused by a temporary error, please try the 'Rerun workflow' button." exit 1 fi @@ -102,18 +103,18 @@ echo "Finished building new images, running run_terraform.sh." run_on_deploy_box "sudo touch /var/log/deploy_$CI_TAG.log" run_on_deploy_box "sudo chown ubuntu:ubuntu /var/log/deploy_$CI_TAG.log" -run_on_deploy_box "source env_vars && echo -e '######\nStarting new deploy for $CI_TAG\n######' >> /var/log/deploy_$CI_TAG.log 2>&1" -run_on_deploy_box "sudo ./.github/scripts/fix_ca_certs.sh >> /var/log/deploy_$CI_TAG.log 2>&1" +run_on_deploy_box ". env_vars && echo -e '######\nStarting new deploy for $CI_TAG\n######' >> /var/log/deploy_$CI_TAG.log 2>&1" +run_on_deploy_box "sudo ./.github/scripts/update_ca_certificates.sh >> /var/log/deploy_$CI_TAG.log 2>&1" # This should never be logged to Github Actions in case it exposes any secrets as terraform output. -run_on_deploy_box "source env_vars && ./.github/scripts/run_terraform.sh >> /var/log/deploy_$CI_TAG.log 2>&1" +run_on_deploy_box ". env_vars && ./.github/scripts/run_terraform.sh >> /var/log/deploy_$CI_TAG.log 2>&1" -run_on_deploy_box "source env_vars && echo -e '######\nDeploying $CI_TAG finished!\n######' >> /var/log/deploy_$CI_TAG.log 2>&1" +run_on_deploy_box ". env_vars && echo -e '######\nDeploying $CI_TAG finished!\n######' >> /var/log/deploy_$CI_TAG.log 2>&1" ./.github/scripts/slackpost_deploy.sh robots deploybot -if [[ "$MASTER_OR_DEV" == "dev" ]]; then - run_on_deploy_box "source env_vars && ./foreman/run_end_to_end_tests.sh" +if [[ "$BRANCH" == "dev" ]]; then + run_on_deploy_box ". env_vars && ./foreman/run_end_to_end_tests.sh" ./.github/scripts/slackpost_end_to_end.sh robots deploybot fi diff --git a/.github/scripts/run_terraform.sh b/.github/scripts/run_terraform.sh index 547b8c208..ed2e0295c 100755 --- a/.github/scripts/run_terraform.sh +++ b/.github/scripts/run_terraform.sh @@ -1,4 +1,6 @@ -#!/bin/bash -e +#!/bin/bash + +set -e # Import Hashicorps' key. curl https://keybase.io/hashicorp/pgp_keys.asc | gpg --import @@ -30,15 +32,16 @@ sudo mv terraform /usr/local/bin/ cd ~/refinebio/infrastructure # Circle won't set the branch name for us, so do it ourselves. +# shellcheck disable=SC1090 . ~/refinebio/scripts/common.sh -branch=$(get_master_or_dev "$CI_TAG") -if [[ $branch == "master" ]]; then - ENVIRONMENT=prod +BRANCH=$(get_deploy_branch "$CI_TAG") +if [[ $BRANCH == "master" ]]; then BATCH_USE_ON_DEMAND_INSTANCES="false" -elif [[ $branch == "dev" ]]; then - ENVIRONMENT=staging + ENVIRONMENT=prod +elif [[ $BRANCH == "dev" ]]; then BATCH_USE_ON_DEMAND_INSTANCES="true" + ENVIRONMENT=staging else echo "Why in the world was run_terraform.sh called from a branch other than dev or master?!" exit 1 @@ -46,4 +49,4 @@ fi # New deployment (use -u circleci since we used to run on CircleCI and we don't # want to recreate all of our resources) -./deploy.sh -e "$ENVIRONMENT" -v "$CI_TAG" -u circleci -i "$BATCH_USE_ON_DEMAND_INSTANCES" +./deploy.sh -e "$ENVIRONMENT" -i "$BATCH_USE_ON_DEMAND_INSTANCES" -u circleci -v "$CI_TAG" diff --git a/.github/scripts/fix_ca_certs.sh b/.github/scripts/update_ca_certificates.sh similarity index 100% rename from .github/scripts/fix_ca_certs.sh rename to .github/scripts/update_ca_certificates.sh diff --git a/.github/scripts/update_docker_image.sh b/.github/scripts/update_docker_image.sh deleted file mode 100755 index a7aa97747..000000000 --- a/.github/scripts/update_docker_image.sh +++ /dev/null @@ -1,85 +0,0 @@ -#!/bin/bash -set -e - -# Load docker_image_exists function and $WORKER_IMAGES. -. ~/refinebio/scripts/common.sh - -# Github won't set the branch name for us, so do it ourselves. -branch=$(get_master_or_dev "$CI_TAG") - -if [[ "$branch" == "master" ]]; then - DOCKERHUB_REPO=ccdl -elif [[ "$branch" == "dev" ]]; then - DOCKERHUB_REPO=ccdlstaging -else - echo "Why in the world was update_docker_image.sh called from a branch other than dev or master!?" - exit 1 -fi - -echo "$CI_TAG" >~/refinebio/common/version - -# Create ~/refinebio/common/dist/data-refinery-common-*.tar.gz, which is -# required by the workers and data_refinery_foreman images. -## Remove old common distributions if they exist. -rm -f ~/refinebio/common/dist/* -cd ~/refinebio/common && python3 setup.py sdist - -# Log into DockerHub. -docker login -u "$DOCKER_ID" -p "$DOCKER_PASSWD" - -cd ~/refinebio -for IMAGE in $WORKER_IMAGES; do - image_name="$DOCKERHUB_REPO/dr_$IMAGE" - if docker_image_exists "$image_name" "$CI_TAG"; then - echo "Docker image exists, skipping: $image_name:$CI_TAG" - else - echo "Building docker image: $image_name:$CI_TAG" - # Build and push image. We use the CI_TAG as the system version. - docker build \ - --build-arg SYSTEM_VERSION="$CI_TAG" \ - --file "workers/dockerfiles/Dockerfile.$IMAGE" \ - --tag "$image_name:$CI_TAG" \ - . - docker push "$image_name:$CI_TAG" - # Update latest version. - docker tag "$image_name:$CI_TAG" "$image_name:latest" - docker push "$image_name:latest" - - # Save some space when we're through. - docker rmi "$image_name:$CI_TAG" - fi -done - -# Build and push foreman image. -FOREMAN_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_foreman" -if docker_image_exists "$FOREMAN_DOCKER_IMAGE" "$CI_TAG"; then - echo "Docker image exists, skipping: $FOREMAN_DOCKER_IMAGE:$CI_TAG" -else - # Build and push image. We use the CI_TAG as the system version. - docker build \ - --build-arg SYSTEM_VERSION="$CI_TAG" \ - --file foreman/dockerfiles/Dockerfile.foreman \ - --tag "$FOREMAN_DOCKER_IMAGE:$CI_TAG" \ - . - docker push "$FOREMAN_DOCKER_IMAGE:$CI_TAG" - # Update latest version. - docker tag "$FOREMAN_DOCKER_IMAGE:$CI_TAG" "$FOREMAN_DOCKER_IMAGE:latest" - docker push "$FOREMAN_DOCKER_IMAGE:latest" -fi - -# Build and push API image. -API_DOCKER_IMAGE="$DOCKERHUB_REPO/dr_api" -if docker_image_exists "$API_DOCKER_IMAGE" "$CI_TAG"; then - echo "Docker image exists, skipping: $API_DOCKER_IMAGE:$CI_TAG" -else - # Build and push image. We use the CI_TAG as the system version. - docker build \ - --build-arg SYSTEM_VERSION="$CI_TAG" \ - --file api/dockerfiles/Dockerfile.api_production \ - --tag "$API_DOCKER_IMAGE:$CI_TAG" \ - . - docker push "$API_DOCKER_IMAGE:$CI_TAG" - # Update latest version. - docker tag "$API_DOCKER_IMAGE:$CI_TAG" "$API_DOCKER_IMAGE:latest" - docker push "$API_DOCKER_IMAGE:latest" -fi diff --git a/.github/scripts/update_docker_images.sh b/.github/scripts/update_docker_images.sh new file mode 100755 index 000000000..06bdabbfd --- /dev/null +++ b/.github/scripts/update_docker_images.sh @@ -0,0 +1,55 @@ +#!/bin/bash + +set -e + +# Load docker_image_exists function and $WORKER_IMAGES. +# shellcheck disable=SC1090 +. ~/refinebio/scripts/common.sh + +# Github won't set the branch name for us, so do it ourselves. +BRANCH=$(get_deploy_branch "$CI_TAG") +DOCKER_ACTION="--push" + +if [[ "$BRANCH" == "master" ]]; then + DOCKERHUB_REPO=ccdl +elif [[ "$BRANCH" == "dev" ]]; then + DOCKERHUB_REPO=ccdlstaging +else + echo "Why in the world was update_docker_images.sh called from a branch other than dev or master!?" + exit 1 +fi + +echo "$CI_TAG" >~/refinebio/common/version + +# Create ~/refinebio/common/dist/data-refinery-common-*.tar.gz, which is +# required by the workers and data_refinery_foreman images. +## Remove old common distributions if they exist. +rm -f ~/refinebio/common/dist/* +cd ~/refinebio/common && python3 setup.py sdist + +cd ~/refinebio + +# Log into DockerHub. +docker login -u "$DOCKER_ID" -p "$DOCKER_PASSWD" + +IMAGE_NAMES="$WORKER_IMAGES foreman api" +for IMAGE_NAME in $IMAGE_NAMES; do + case $IMAGE_NAME in + api) + DOCKER_FILE_PATH="api/dockerfiles/Dockerfile.api_production" + ;; + foreman) + DOCKER_FILE_PATH="foreman/dockerfiles/Dockerfile.$IMAGE_NAME" + ;; + *) + DOCKER_FILE_PATH="workers/dockerfiles/Dockerfile.$IMAGE_NAME" + ;; + esac + + if docker_image_exists "$IMAGE_NAME" "$CI_TAG"; then + echo "Docker image exists, skipping: $IMAGE_NAME:$CI_TAG" + else + echo "Building the $IMAGE_NAME:$CI_TAG image from $DOCKER_FILE_PATH." + update_docker_image "$DOCKERHUB_REPO" "$IMAGE_NAME" "$CI_TAG" "$DOCKER_FILE_PATH" "$DOCKER_ACTION" + fi +done diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 902cd0765..fbe8dbec3 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -3,7 +3,6 @@ name: refine.bio CI/CD on: push env: - CODECOV_TOKEN: ${{ secrets.CODECOV_TOKEN }} DEPLOY_IP_ADDRESS: ${{ secrets.DEPLOY_IP_ADDRESS }} DOCKER_BUILDKIT: 1 DOCKER_ID: ${{ secrets.DOCKER_ID }} @@ -142,7 +141,13 @@ jobs: mkdir -p test_volume sudo chmod -R a+rw test_volume api/run_tests.sh - .github/scripts/upload_test_coverage.sh api + .github/scripts/prepare_test_coverage.sh api + + - name: Upload Test Coverage to Codecov + uses: codecov/codecov-action@v3 + with: + files: ./test_volume/api_coverage.xml + name: API Tests test_api_base: name: Test API Base @@ -246,7 +251,13 @@ jobs: mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume common/run_tests.sh - .github/scripts/upload_test_coverage.sh common + .github/scripts/prepare_test_coverage.sh common + + - name: Upload Test Coverage to Codecov + uses: codecov/codecov-action@v3 + with: + files: ./test_volume/common_coverage.xml + name: Common Tests - name: Login to Packages Container Registry uses: docker/login-action@v2 @@ -334,7 +345,13 @@ jobs: mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume foreman/run_tests.sh --exclude-tag=end_to_end - .github/scripts/upload_test_coverage.sh foreman + .github/scripts/prepare_test_coverage.sh foreman + + - name: Upload Test Coverage to Codecov + uses: codecov/codecov-action@v3 + with: + files: ./test_volume/foreman_coverage.xml + name: Foreman Tests - name: Login to Packages Container Registry uses: docker/login-action@v2 @@ -379,7 +396,13 @@ jobs: mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t illumina - .github/scripts/upload_test_coverage.sh workers + .github/scripts/prepare_test_coverage.sh workers + + - name: Upload Test Coverage to Codecov + uses: codecov/codecov-action@v3 + with: + files: ./workers/test_volume/workers_coverage.xml + name: Illumina Tests - name: Login to Packages Container Registry uses: docker/login-action@v2 @@ -423,7 +446,13 @@ jobs: run: | sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t salmon - .github/scripts/upload_test_coverage.sh workers + .github/scripts/prepare_test_coverage.sh workers + + - name: Upload Test Coverage to Codecov + uses: codecov/codecov-action@v3 + with: + files: ./workers/test_volume/workers_coverage.xml + name: Salmon Tests - name: Login to Packages Container Registry uses: docker/login-action@v2 @@ -468,25 +497,43 @@ jobs: mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t smasher - .github/scripts/upload_test_coverage.sh workers + .github/scripts/prepare_test_coverage.sh workers - name: Run Janitor Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t janitor - .github/scripts/upload_test_coverage.sh workers + .github/scripts/prepare_test_coverage.sh workers + + - name: Upload Test Coverage to Codecov + uses: codecov/codecov-action@v3 + with: + files: ./workers/test_volume/workers_coverage.xml + name: Janitor Tests - name: Run Compendia Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t compendia - .github/scripts/upload_test_coverage.sh workers + .github/scripts/prepare_test_coverage.sh workers + + - name: Upload Test Coverage to Codecov + uses: codecov/codecov-action@v3 + with: + files: ./workers/test_volume/workers_coverage.xml + name: Compendia Tests - name: Run QN Tests run: | sudo -E chown -R $USER:$USER workers/test_volume .github/scripts/filter_tests.sh -t qn - .github/scripts/upload_test_coverage.sh workers + .github/scripts/prepare_test_coverage.sh workers + + - name: Upload Test Coverage to Codecov + uses: codecov/codecov-action@v3 + with: + files: ./workers/test_volume/workers_coverage.xml + name: QN Tests - name: Login to Packages Container Registry uses: docker/login-action@v2 @@ -532,7 +579,13 @@ jobs: mkdir -p workers/test_volume sudo chmod -R a+rw workers/test_volume .github/scripts/filter_tests.sh -t transcriptome - .github/scripts/upload_test_coverage.sh workers + .github/scripts/prepare_test_coverage.sh workers + + - name: Upload Test Coverage to Codecov + uses: codecov/codecov-action@v3 + with: + files: ./workers/test_volume/workers_coverage.xml + name: Transcriptome Tests - name: Login to Packages Container Registry uses: docker/login-action@v2 diff --git a/scripts/common.sh b/scripts/common.sh index b30416abc..acf22354d 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -39,20 +39,20 @@ get_docker_es_ip_address() { # A tag is linked to a commit hash, not a branch. A single commit hash # can end up on multiple branches. So we first check to see if we're # on master, then on dev, then error out because we should only deploy master or dev. -get_master_or_dev() { +get_deploy_branch() { # Takes the version that is being deployed as its only parameter version="$1" if [ -z "$version" ]; then - echo "You must pass the version to get_master_or_dev." + echo "You must pass the version to get_deploy_branch()." else - master_check=$(git log origin/master --decorate=full | grep "$version" || true) - dev_check=$(git log origin/dev --decorate=full | grep "$version" || true) + is_master=$(git log origin/master --decorate=full | grep "$version" || true) + is_dev=$(git log origin/dev --decorate=full | grep "$version" || true) # All dev versions should end with '-dev' or '-dev-hotfix' and all master versions should not. - if [ -n "$master_check" ] && ! echo "$version" | grep -Eq "\-dev(\-hotfix)?$"; then + if [ -n "$is_master" ] && ! echo "$version" | grep -Eq "\-dev(\-hotfix)?$"; then echo "master" - elif [ -n "$dev_check" ]; then + elif [ -n "$is_dev" ]; then echo "dev" else echo "unknown" @@ -118,3 +118,43 @@ EOF echo "Using Docker builder $DOCKER_BUILDER." docker buildx use "$DOCKER_BUILDER" } + +update_docker_image() { + DOCKERHUB_REPO="$1" + IMAGE_NAME="$2" + SYSTEM_VERSION="$3" + DOCKER_FILE_PATH="$4" + DOCKER_ACTION="$5" + + if [ -z "$DOCKER_ACTION" ]; then + DOCKER_ACTION="--load" + fi + + DOCKERHUB_IMAGE="$DOCKERHUB_REPO/dr_$IMAGE_NAME" + CACHE_FROM_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest" + CACHE_FROM_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION" + CACHE_TO_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" + CACHE_TO_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" + + if test "$GITHUB_ACTION"; then + CACHE_TO_LATEST="type=gha" + CACHE_TO_VERSION="type=gha" + DOCKER_ACTION="--push" + fi + + set_up_docker_builder + + docker buildx build \ + --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ + --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ + --cache-from "$CACHE_FROM_LATEST" \ + --cache-from "$CACHE_FROM_VERSION" \ + --cache-to "$CACHE_TO_LATEST" \ + --cache-to "$CACHE_TO_VERSION" \ + --file "$DOCKER_FILE_PATH" \ + --platform linux/amd64 \ + --tag "$DOCKERHUB_IMAGE:latest" \ + --tag "$DOCKERHUB_IMAGE:$SYSTEM_VERSION" \ + "$DOCKER_ACTION" \ + . +} diff --git a/scripts/prepare_image.sh b/scripts/prepare_image.sh index d9a49c68d..f6c4c8af9 100755 --- a/scripts/prepare_image.sh +++ b/scripts/prepare_image.sh @@ -8,6 +8,7 @@ script_directory="$( )" cd "$script_directory" || exit +# shellcheck disable=SC1091 . ./common.sh # We need access to all of the projects @@ -89,54 +90,24 @@ if [ -z "$DOCKER_ACTION" ]; then DOCKER_ACTION="--load" fi -DOCKERHUB_IMAGE="$DOCKERHUB_REPO/dr_$IMAGE_NAME" - -CACHE_FROM_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest" -CACHE_FROM_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION" -CACHE_TO_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" -CACHE_TO_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" - -if test "$GITHUB_ACTION"; then - CACHE_TO_LATEST="type=gha" - CACHE_TO_VERSION="type=gha" - DOCKER_ACTION="--push" -fi - DOCKER_FILE_PATH="$SERVICE/dockerfiles/Dockerfile.$IMAGE_NAME" -echo -echo "Building the $IMAGE_NAME:$SYSTEM_VERSION image from $DOCKER_FILE_PATH." -echo - attempt=0 -attempts=3 +max_attempts=3 finished=1 -while [ $finished != 0 ] && [ $attempt -lt $attempts ]; do +while [ $finished != 0 ] && [ $attempt -lt $max_attempts ]; do if [ $attempt -gt 0 ]; then echo "Failed to build $IMAGE_NAME:$SYSTEM_VERSION image, trying again." fi - set_up_docker_builder - - docker buildx build \ - --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ - --cache-from "$CACHE_FROM_LATEST" \ - --cache-from "$CACHE_FROM_VERSION" \ - --cache-to "$CACHE_TO_LATEST" \ - --cache-to "$CACHE_TO_VERSION" \ - --file "$DOCKER_FILE_PATH" \ - --platform linux/amd64 \ - --tag "$DOCKERHUB_IMAGE:latest" \ - --tag "$DOCKERHUB_IMAGE:$SYSTEM_VERSION" \ - "$DOCKER_ACTION" \ - . + echo "Building the $IMAGE_NAME:$SYSTEM_VERSION image from $DOCKER_FILE_PATH." + update_docker_image "$DOCKERHUB_REPO" "$IMAGE_NAME" "$SYSTEM_VERSION" "$DOCKER_FILE_PATH" "$DOCKER_ACTION" finished=$? attempt=$((attempt + 1)) done -if [ $finished -ne 0 ] && [ $attempt -ge $attempts ]; then +if [ $finished -ne 0 ] && [ $attempt -ge $max_attempts ]; then echo "Could not build $DOCKERHUB_IMAGE after $attempt attempts." exit 1 fi diff --git a/scripts/update_docker_images.sh b/scripts/update_docker_images.sh index d3910854a..299974310 100755 --- a/scripts/update_docker_images.sh +++ b/scripts/update_docker_images.sh @@ -1,6 +1,5 @@ #!/bin/sh -# Exit on failure. set -e # This script should always run as if it were being called from @@ -11,6 +10,7 @@ script_directory="$( )" cd "$script_directory" || exit +# shellcheck disable=SC1091 . ./common.sh # Get access to all of refinebio. @@ -74,12 +74,14 @@ if [ -z "$SYSTEM_VERSION" ]; then SYSTEM_VERSION="$(get_branch_hash)" fi +DOCKER_ACTION="--push" + # Intentionally omit affymetrix unless specifically requested since it is so # intense to build. -image_names="base migrations common_tests foreman api_base api_production api_local \ +IMAGE_NAMES="base migrations common_tests foreman api_base api_production api_local \ transcriptome smasher salmon no_op illumina downloaders compendia" if [ "$BUILD_AFFYMETRIX" ]; then - image_names="$image_names affymetrix" + IMAGE_NAMES="$IMAGE_NAMES affymetrix" fi # Set the version for the common project. @@ -91,46 +93,22 @@ echo "$SYSTEM_VERSION" >common/version rm -f common/dist/* (cd common && python3 setup.py sdist 1>/dev/null) # Run quietly in a subshell. -# shellcheck disable=SC2086 -for image_name in $image_names; do - case $image_name in +for IMAGE_NAME in $IMAGE_NAMES; do + case $IMAGE_NAME in api_base | api_local | api_production) - DOCKER_FILE_PATH="api/dockerfiles/Dockerfile.$image_name" + DOCKER_FILE_PATH="api/dockerfiles/Dockerfile.$IMAGE_NAME" ;; base | common_tests | migrations) - DOCKER_FILE_PATH="common/dockerfiles/Dockerfile.$image_name" + DOCKER_FILE_PATH="common/dockerfiles/Dockerfile.$IMAGE_NAME" ;; foreman) - DOCKER_FILE_PATH="foreman/dockerfiles/Dockerfile.$image_name" + DOCKER_FILE_PATH="foreman/dockerfiles/Dockerfile.$IMAGE_NAME" ;; *) - DOCKER_FILE_PATH="workers/dockerfiles/Dockerfile.$image_name" + DOCKER_FILE_PATH="workers/dockerfiles/Dockerfile.$IMAGE_NAME" ;; esac - echo - echo "Building the $image_name:$SYSTEM_VERSION image from $DOCKER_FILE_PATH." - echo - - DOCKERHUB_IMAGE="$DOCKERHUB_REPO/dr_$image_name" - CACHE_FROM_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest" - CACHE_FROM_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION" - CACHE_TO_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" - CACHE_TO_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" - - set_up_docker_builder - - docker buildx build \ - --build-arg DOCKERHUB_REPO="$DOCKERHUB_REPO" \ - --build-arg SYSTEM_VERSION="$SYSTEM_VERSION" \ - --cache-from "$CACHE_FROM_LATEST" \ - --cache-from "$CACHE_FROM_VERSION" \ - --cache-to "$CACHE_TO_LATEST" \ - --cache-to "$CACHE_TO_VERSION" \ - --file "$DOCKER_FILE_PATH" \ - --platform linux/amd64 \ - --push \ - --tag "$DOCKERHUB_IMAGE:latest" \ - --tag "$DOCKERHUB_IMAGE:$SYSTEM_VERSION" \ - . + echo "Building the $IMAGE_NAME:$SYSTEM_VERSION image from $DOCKER_FILE_PATH." + update_docker_image "$DOCKERHUB_REPO" "$IMAGE_NAME" "$SYSTEM_VERSION" "$DOCKER_FILE_PATH" "$DOCKER_ACTION" done From 62039f6945e52ff63d7fb3732efa619972f06339 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 12 Jul 2023 11:20:16 -0700 Subject: [PATCH 157/220] Fix flaky API throttling test Update test_api_general::APITestCases::test_all_endpoints --- api/tests/views/test_api_general.py | 9 +++++++-- 1 file changed, 7 insertions(+), 2 deletions(-) diff --git a/api/tests/views/test_api_general.py b/api/tests/views/test_api_general.py index 8d0b76358..fd817ccd2 100644 --- a/api/tests/views/test_api_general.py +++ b/api/tests/views/test_api_general.py @@ -347,9 +347,14 @@ def test_all_endpoints(self): # Tenth call since reset_cache() should be throttled, three have happened. # Make more than necessary to ensure we get the throttle. - for i in range(15): + response_codes = [] + for _ in range(15): response = self.client.get(reverse("survey_jobs", kwargs={"version": API_VERSION})) - self.assertEqual(response.status_code, status.HTTP_429_TOO_MANY_REQUESTS) + response_codes.append(response.status_code) + self.assertGreater( + len([rc for rc in response_codes if rc == status.HTTP_429_TOO_MANY_REQUESTS]), + 0, + ) def test_experiment_multiple_accessions(self): response = self.client.get( From 7ff4becc1db277634e8bd47c2d5f8baa91386846 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 14 Jul 2023 13:46:59 -0400 Subject: [PATCH 158/220] sort multiple values alphanumerical ascending --- foreman/data_refinery_foreman/surveyor/harmony.py | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 123ef5236..f706df68e 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -787,7 +787,9 @@ def harmonize_field( if sample_attribute == "age" and len(harmonized_values) > 0: harmonized_sample[sample_attribute] = harmonized_values.pop() else: - harmonized_sample[sample_attribute] = ";".join([str(v) for v in harmonized_values]) + harmonized_sample[sample_attribute] = ";".join( + sorted([str(v) for v in harmonized_values]) + ) def harmonize_sample(self, sample_metadata: Dict, title_field: str = None) -> Dict: variants_attributes = [ From a5e78e1649c7dbc785e4d87a9b814fffb001f8ef Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 14 Jul 2023 14:42:21 -0400 Subject: [PATCH 159/220] add util functions for SRA --- .../data_refinery_foreman/surveyor/utils.py | 24 ++++++++++++++ foreman/tests/surveyor/test_utils.py | 31 +++++++++++++++++++ 2 files changed, 55 insertions(+) create mode 100644 foreman/tests/surveyor/test_utils.py diff --git a/foreman/data_refinery_foreman/surveyor/utils.py b/foreman/data_refinery_foreman/surveyor/utils.py index d14c7d7df..7992dac33 100644 --- a/foreman/data_refinery_foreman/surveyor/utils.py +++ b/foreman/data_refinery_foreman/surveyor/utils.py @@ -1,4 +1,5 @@ import collections +from typing import Dict, List, Union import requests from requests.adapters import HTTPAdapter @@ -43,6 +44,29 @@ def flatten(d, parent_key="", sep="_"): return dict(items) +# TODO: Merge this with above method after updating array express +# to support recursive flattening. +def flatten_dict(data: Union[Dict, List], prefix: str = "", sep="_") -> dict: + """Flattens a nested dictionary or list""" + flattened = {} + iterator = enumerate(data) if isinstance(data, list) else data.items() + + for k, v in iterator: + key = (k if not prefix else f"{prefix}{sep}{k}").replace(" ", sep) + if isinstance(v, dict) or isinstance(v, list): + flattened.update(flatten_dict(v, key)) + else: + flattened[key] = v + + return flattened + + +def get_nonempty(hash: dict, key: str, fallback): + if key in hash and hash[key]: + return hash[key] + return fallback + + def get_title_and_authors_for_pubmed_id(pmid): """Given a PMID, return that PMID's (title, [authors]).""" diff --git a/foreman/tests/surveyor/test_utils.py b/foreman/tests/surveyor/test_utils.py new file mode 100644 index 000000000..4228173c5 --- /dev/null +++ b/foreman/tests/surveyor/test_utils.py @@ -0,0 +1,31 @@ +from django.test import TestCase + +from data_refinery_foreman.surveyor import utils + + +class UtilsTestCase(TestCase): + def test_flatten_dict(self): + """ + Tests that flattening dictionaries and lists behaves as expected. + """ + source_dict = { + "first foo": "first_value", + "second bar": { + "nested": True, + }, + "third fuzz": ["another_nested", {"nested_object": True}], + } + + flattened_dict = utils.flatten_dict(source_dict) + + self.assertEqual(flattened_dict["first_foo"], "first_value") + self.assertEqual(flattened_dict["second_bar_nested"], True) + self.assertEqual(flattened_dict["third_fuzz_0"], "another_nested") + self.assertEqual(flattened_dict["third_fuzz_1_nested_object"], True) + + prefix_flattened_dict = utils.flatten_dict(source_dict, "test") + + self.assertEqual(prefix_flattened_dict["test_first_foo"], "first_value") + self.assertEqual(prefix_flattened_dict["test_second_bar_nested"], True) + self.assertEqual(prefix_flattened_dict["test_third_fuzz_0"], "another_nested") + self.assertEqual(prefix_flattened_dict["test_third_fuzz_1_nested_object"], True) From 11d90c6adb686e06c3edcd6d8562114cc7d89684 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 14 Jul 2023 14:42:47 -0400 Subject: [PATCH 160/220] update cassette to new json response --- ...or.sra.metadata_is_gathered_correctly.yaml | 325 +++++------------- 1 file changed, 93 insertions(+), 232 deletions(-) diff --git a/test_volume/cassettes/surveyor.sra.metadata_is_gathered_correctly.yaml b/test_volume/cassettes/surveyor.sra.metadata_is_gathered_correctly.yaml index 2e31cff57..6608b8d63 100644 --- a/test_volume/cassettes/surveyor.sra.metadata_is_gathered_correctly.yaml +++ b/test_volume/cassettes/surveyor.sra.metadata_is_gathered_correctly.yaml @@ -9,44 +9,43 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.30.0 + - python-requests/2.31.0 method: GET - uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRR002116 + uri: https://www.ebi.ac.uk/ena/portal/api/search/?format=json&includeAccessions=DRR002116&result=read_run&fields=all response: body: - string: "\n\n\n \n DRR002116\n - \ DRR002116\n - \ \n Illumina HiSeq 2000 sequencing; Exp_Gg_HH16_1_embryo_mRNAseq\n - \ \n - \ \n \n \n ENA-STUDY\n - \ DRP000595\n \n \n - \ \n \n ENA-SAMPLE\n - \ DRS001521\n \n \n - \ \n \n ENA-SUBMISSION\n - \ DRA000567\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n \n - \ \n ENA-SPOT-COUNT\n 32568360\n - \ \n \n ENA-BASE-COUNT\n - \ 3256836000\n \n \n - \ ENA-FIRST-PUBLIC\n 2013-07-19\n - \ \n \n ENA-LAST-UPDATE\n - \ 2017-08-11\n \n \n\n\n" + string: '[ + + {"run_accession":"DRR002116","submission_accession":"DRA000567","library_min_fragment_size":"","bam_md5":"","assembly_software":"","library_prep_longitude":"","library_selection":"RANDOM","pcr_isolation_protocol":"","chip_protocol":"","sequencing_primer_provider":"","serotype":"","environment_feature":"","last_updated":"2017-08-11","submitted_galaxy":"","extraction_protocol":"","germline":"","secondary_project":"","culture_collection":"","submission_tool":"","sra_bytes":"2324796808","read_strand":"","rna_purity_280_ratio":"","hi_c_protocol":"","collected_by":"","submitted_ftp":"","restriction_enzyme_target_sequence":"","isolate":"","fastq_bytes":"2969261051","instrument_platform":"ILLUMINA","variety":"","sequencing_date_format":"","temperature":"","sra_aspera":"fasp.sra.ebi.ac.uk:/vol1/drr/DRR002/DRR002116","ecotype":"","submitted_aspera":"","sampling_campaign":"","bam_ftp":"","tissue_lib":"","environmental_sample":"","control_experiment":"","sex":"","submitted_md5":"","checklist":"","fastq_galaxy":"ftp.sra.ebi.ac.uk/vol1/fastq/DRR002/DRR002116/DRR002116.fastq.gz","library_gen_protocol":"","specimen_voucher":"","library_prep_latitude":"","submitted_bytes":"","taxonomic_identity_marker":"","run_date":"1314831600000","country":"","ncbi_reporting_standard":"Generic","sample_description":"Gg_HH16_1_embryo_mRNAseq","experiment_title":"Illumina 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Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked + X-Content-Type-Options: + - nosniff + X-Frame-Options: + - DENY + X-XSS-Protection: + - 1; mode=block status: code: 200 message: '' @@ -60,67 +59,46 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.30.0 + - python-requests/2.31.0 method: GET - uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRX001563 + uri: https://www.ebi.ac.uk/ena/portal/api/search/?format=json&includeAccessions=DRP000595&result=study&fields=all response: body: - string: "\n\n\n \n - \ DRX001563\n DRX001563\n - \ \n Illumina HiSeq 2000 sequencing; Exp_Gg_HH16_1_embryo_mRNAseq\n - \ \n - \ \n DRP000595\n - \ PRJDB90\n - \ DRP000595\n - \ \n \n \n Experiment - for mRNAseq of chicken at stage HH16 (biological replicate 1)\n - \ \n \n DRS001521\n - \ SAMD00004104\n - \ DRS001521\n - \ \n \n \n - \ Gg_HH16_1_embryo_mRNAseq\n RNA-Seq\n - \ TRANSCRIPTOMIC\n RANDOM\n - \ \n \n \n - \ \n \n \n - \ 100\n \n - \ 0\n Application - Read\n Forward\n - \ 1\n \n - \ \n \n \n - \ \n \n Illumina - HiSeq 2000\n \n \n \n - \ \n \n 1\n - \ NIL\n \n - \ \n \n \n - \ \n \n \n - \ \n ENA-SAMPLE\n DRS001521\n - \ \n \n \n - \ \n ENA-RUN\n DRR002116\n - \ \n \n \n - \ \n ENA-SUBMISSION\n - \ DRA000567\n \n \n - \ \n \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n ENA-SPOT-COUNT\n - \ 32568360\n \n - \ \n ENA-BASE-COUNT\n - \ 3256836000\n \n - \ \n\n\n" + string: '[ + + {"accession":"PRJDB90","keywords":"","description":"Evolutionary origin of + turtles, and Evo-Devo aspect of turtle shell were investigated in this project.","study_name":"Pelodiscus + sinensis japonicus strain:Eggs bought from local farmer in Japan","geo_accession":"","isolate":"","center_name":"RIKEN + Center for Developmental Biology","secondary_study_accession":"DRP000670;DRP000595","tag":"ena","secondary_study_center_name":"","strain":"Eggs + bought from local farmer in Japan","study_title":"P. sinensis EvoDevo project","last_updated":"2023-05-19","first_public":"2013-12-14","broker_name":"","study_description":"Evolutionary + origin of turtles, and Evo-Devo aspect of turtle shell were investigated in + this project.","secondary_study_alias":"DRP000670;DRP000595","scientific_name":"Pelodiscus + sinensis japonicus","study_alias":"PRJDB90","breed":"","tax_id":"34908","parent_study_accession":"PRJDB1384;PRJNA171777","study_accession":"PRJDB90","submission_tool":"","cultivar":"","tax_division":"VRT","status":"public"} + + ] + + ' headers: + Cache-Control: + - no-cache, no-store, max-age=0, must-revalidate + Content-Length: + - '1025' Content-Type: - - application/xml + - application/json Date: - - Wed, 17 May 2023 18:36:44 GMT + - Wed, 07 Jun 2023 20:31:08 GMT + Expires: + - '0' + Pragma: + - no-cache Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked + X-Content-Type-Options: + - nosniff + X-Frame-Options: + - DENY + X-XSS-Protection: + - 1; mode=block status: code: 200 message: '' @@ -134,164 +112,47 @@ interactions: Connection: - keep-alive User-Agent: - - python-requests/2.30.0 + - python-requests/2.31.0 method: GET - uri: https://www.ebi.ac.uk/ena/browser/api/xml/DRS001521 + uri: https://www.ebi.ac.uk/biosamples/samples/SAMD00004104?format=json response: body: - string: "\n\n\n - \ \n DRS001521\n SAMD00004104\n \n - \ Gg_HH16_1_embryo_mRNAseq\n \n 9031\n - \ Gallus gallus\n chicken\n - \ \n \n \n \n - \ ENA-FASTQ-FILES\n \n - \ \n \n \n \n - \ ENA-SUBMITTED-FILES\n \n - \ \n \n\n\n - \ \n INSDC secondary accession\n - \ DRS001521\n \n \n - \ NCBI submission package\n Generic.1.0\n - \ \n \n organism\n - \ Gallus gallus\n \n - \ \n sample comment\n mRNAseq - of chicken at stage HH16 (biological replicate 1)\n \n - \ \n sample name\n DRS001521\n - \ \n \n BioSampleModel\n - \ Generic\n \n \n - \ ENA-FIRST-PUBLIC\n 2013-02-27\n - \ \n \n ENA-LAST-UPDATE\n - \ 2014-11-12\n \n - \ \n\n\n" - headers: - Content-Type: - - application/xml - Date: - - 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\n \n - \ \n ENA-FASTQ-FILES\n - \ \n - \ \n \n \n - \ \n ENA-SUBMITTED-FILES\n - \ \n - \ \n \n \n - \ \n \n ENA-SPOT-COUNT\n - \ 1371813555\n \n - \ \n ENA-BASE-COUNT\n - \ 158881910957\n \n - \ \n\n\n" + RIKEN"}],"INSDC first public":[{"text":"2013-02-27T00:00:00Z"}],"INSDC last + update":[{"text":"2014-11-12T03:28:52Z"}],"INSDC secondary accession":[{"text":"DRS001521"}],"INSDC + status":[{"text":"live"}],"NCBI submission model":[{"text":"Generic"}],"NCBI + submission package":[{"text":"Generic.1.0"}],"SRA accession":[{"text":"DRS001521"}],"organism":[{"text":"Gallus + gallus","ontologyTerms":["http://purl.obolibrary.org/obo/NCBITaxon_9031"]}],"sample + comment":[{"text":"mRNAseq of chicken at stage HH16 (biological replicate + 1)","tag":"attribute"}],"sample name":[{"text":"DRS001521","tag":"attribute"}],"title":[{"text":"Gg_HH16_1_embryo_mRNAseq"}]},"externalReferences":[{"url":"https://www.ebi.ac.uk/ena/data/view/SAMD00004104","duo":null}],"submittedVia":"JSON_API","create":"2013-07-03T08:05:45.306Z","_links":{"self":{"href":"https://www.ebi.ac.uk/biosamples/samples/SAMD00004104"},"curationDomain":{"href":"https://www.ebi.ac.uk/biosamples/samples/SAMD00004104{?curationdomain}","templated":true},"curationLinks":{"href":"https://www.ebi.ac.uk/biosamples/samples/SAMD00004104/curationlinks"},"curationLink":{"href":"https://www.ebi.ac.uk/biosamples/samples/SAMD00004104/curationlinks/{hash}","templated":true},"structuredData":{"href":"https://www.ebi.ac.uk/biosamples/structureddata/SAMD00004104"}}}' headers: + Cache-Control: + - max-age=60, public + Content-Length: + - '1732' Content-Type: - - application/xml + - application/json;charset=UTF-8 Date: - - Wed, 17 May 2023 18:36:45 GMT + - Wed, 07 Jun 2023 20:31:08 GMT + ETag: + - '"092374b249b8b19f3dcc3ac478762eae2"' Strict-Transport-Security: - max-age=0 - Transfer-Encoding: - - chunked + Vary: + - Origin + - Access-Control-Request-Method + - Access-Control-Request-Headers + - Origin + - Access-Control-Request-Method + - Access-Control-Request-Headers + X-Content-Type-Options: + - nosniff + X-Frame-Options: + - DENY + X-XSS-Protection: + - 1; mode=block status: code: 200 message: '' From 5fbecefa7d36a61462edeb610e203749fd837b09 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 14 Jul 2023 14:43:21 -0400 Subject: [PATCH 161/220] add utils to import tests --- foreman/tests/test_imports.py | 1 + 1 file changed, 1 insertion(+) diff --git a/foreman/tests/test_imports.py b/foreman/tests/test_imports.py index 9c2717e0e..1544f77d9 100644 --- a/foreman/tests/test_imports.py +++ b/foreman/tests/test_imports.py @@ -57,4 +57,5 @@ def test_imports(self): import tests.surveyor.test_harmony import tests.surveyor.test_sra import tests.surveyor.test_surveyor + import tests.surveyor.test_utils import tests.surveyor.test_transcriptome_index From 6581d7f1f29ca421aededcc9bab881add6026961 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 14 Jul 2023 14:50:00 -0400 Subject: [PATCH 162/220] rework to support new ENA endpoint --- foreman/data_refinery_foreman/surveyor/sra.py | 768 ++++++------------ 1 file changed, 269 insertions(+), 499 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index d414dbffa..bff26a741 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -1,6 +1,5 @@ import re -import xml.etree.ElementTree as ET -from typing import Dict, List +from typing import List from django.utils.dateparse import parse_date @@ -25,12 +24,11 @@ DOWNLOAD_SOURCE = "NCBI" # or "ENA". Change this to download from NCBI (US) or ENA (UK). -ENA_URL_TEMPLATE = "https://www.ebi.ac.uk/ena/browser/view/{}" -ENA_METADATA_URL_TEMPLATE = "https://www.ebi.ac.uk/ena/browser/api/xml/{}" -ENA_FILE_REPORT_URL_TEMPLATE = ( - "https://www.ebi.ac.uk/ena/portal/api/filereport?accession={accession}&result=read_run" -) NCBI_DOWNLOAD_URL_TEMPLATE = "https://sra-pub-run-odp.s3.amazonaws.com/sra/{accession}/{accession}" +ENA_URL_TEMPLATE = "https://www.ebi.ac.uk/ena/browser/view/{}" +ENA_API = "https://www.ebi.ac.uk/ena/portal/api/" +ENA_BIOSAMPLE_API = "https://www.ebi.ac.uk/biosamples/samples" +ENA_PUBMED_API_TEMPLATE = "https://www.ebi.ac.uk/ena/xref/rest/json/search?accession={accession}&expanded=true&source=PubMed" class UnsupportedDataTypeError(Exception): @@ -56,275 +54,137 @@ def source_type(self): return Downloaders.SRA.value @staticmethod - def gather_submission_metadata(metadata: Dict) -> None: - formatted_metadata_URL = ENA_METADATA_URL_TEMPLATE.format(metadata["submission_accession"]) - response = utils.requests_retry_session().get(formatted_metadata_URL) - submission_xml = ET.fromstring(response.text)[0] - submission_metadata = submission_xml.attrib - - # We already have these - submission_metadata.pop("accession", "") - submission_metadata.pop("alias", "") + def get_ena_json_api(endpoint: str, accession, params: dict = {}): + query_params = "&".join([f"{k}={params[k]}" for k in params]) - metadata.update(submission_metadata) + if query_params: + query_params = f"&{query_params}" - for child in submission_xml: - if child.tag == "TITLE": - metadata["submission_title"] = child.text - elif child.tag == "SUBMISSION_ATTRIBUTES": - for grandchild in child: - metadata[grandchild.find("TAG").text.lower()] = grandchild.find("VALUE").text + return f"{ENA_API}{endpoint}/?format=json&includeAccessions={accession}{query_params}" @staticmethod - def gather_library_metadata(metadata: Dict, library: ET.Element) -> None: - for child in library: - if child.tag == "LIBRARY_LAYOUT": - metadata["library_layout"] = child[0].tag - else: - metadata[child.tag.lower()] = child.text - - # SRA contains several types of data. We only want RNA-Seq for now. - if metadata["library_strategy"] != "RNA-Seq": - raise UnsupportedDataTypeError("library_strategy not RNA-Seq.") - if metadata["library_source"] not in ["TRANSCRIPTOMIC", "OTHER"]: - raise UnsupportedDataTypeError( - "library_source: " + metadata["library_source"] + " not TRANSCRIPTOMIC or OTHER." + def get_ena_json_api_response(endpoint: str, accession, params: dict = {}): + response = utils.requests_retry_session().get( + SraSurveyor.get_ena_json_api(endpoint, accession, params) + ) + # handle logging of errors here consistently + if response.status_code != 200: + logger.error( + "Could not discover {}.".format(endpoint), + accession=accession, + status_code=response.status_code, + url=response.url, ) + return {} + return response.json() @staticmethod - def parse_read_spec(metadata: Dict, read_spec: ET.Element, counter: int) -> None: - for child in read_spec: - key = "read_spec_{}_{}".format(str(counter), child.tag.replace("READ_", "").lower()) - metadata[key] = child.text + def gather_experiment_metadata(experiment_accession: str): + experiments = SraSurveyor.get_ena_json_api_response( + "search", + experiment_accession, + { + "result": "study", + "fields": "all", + }, + ) + if len(experiments) > 0: + return experiments[0] + return {} @staticmethod - def gather_spot_metadata(metadata: Dict, spot: ET.Element) -> None: - """We don't actually know what this metadata is about. - - SPOT_DECODE_SPECs have a variable number of READ_SPECs so we - have to add a counter to the keys in order to make them work - within the flat dictionary we're building. - """ - for child in spot: - if child.tag == "SPOT_DECODE_SPEC": - read_spec_counter = 0 - for grandchild in child: - if grandchild.tag == "SPOT_LENGTH": - metadata["spot_length"] = grandchild.text - elif grandchild.tag == "READ_SPEC": - SraSurveyor.parse_read_spec(metadata, grandchild, read_spec_counter) - read_spec_counter = read_spec_counter + 1 + def gather_experiment_runs_metadata(accession: str): + # if accession is experiment it will return all samples + # if accession is sample it will return just the sample + samples_metadata = SraSurveyor.get_ena_json_api_response( + "search", + accession, + { + "result": "read_run", + "fields": "all", + }, + ) - @staticmethod - def gather_experiment_metadata(metadata: Dict) -> None: - formatted_metadata_URL = ENA_METADATA_URL_TEMPLATE.format(metadata["experiment_accession"]) - response = utils.requests_retry_session().get(formatted_metadata_URL) - experiment_xml = ET.fromstring(response.text) - - experiment = experiment_xml[0] - for child in experiment: - if child.tag == "TITLE": - metadata["experiment_title"] = child.text - elif child.tag == "DESIGN": - for grandchild in child: - if grandchild.tag == "DESIGN_DESCRIPTION": - metadata["experiment_design_description"] = grandchild.text - elif grandchild.tag == "LIBRARY_DESCRIPTOR": - SraSurveyor.gather_library_metadata(metadata, grandchild) - elif grandchild.tag == "SPOT_DESCRIPTOR": - SraSurveyor.gather_spot_metadata(metadata, grandchild) - elif child.tag == "PLATFORM": - # This structure is extraneously nested. - metadata["platform_instrument_model"] = child[0][0].text + samples_metadata = [SraSurveyor.gather_biosample_metadata(s) for s in samples_metadata] - @staticmethod - def parse_run_link(run_link: ET.ElementTree) -> (str, str): - key = "" - value = "" - - # The first level in this element is a XREF_LINK which is - # really just an unnecessary level - for child in run_link[0]: - if child.tag == "DB": - # The key is prefixed with "ena-" which is unnecessary - # since we know this is coming from ENA - key = child.text.lower().replace("ena-", "") + "_accession" - elif child.tag == "ID": - value = child.text - - return (key, value) + return samples_metadata @staticmethod - def parse_attribute(attribute: ET.ElementTree, key_prefix: str = "") -> (str, str): - """Parse an XML attribute. - - Takes an optional key_prefix which is used to differentiate - keys which may be shared between different object types. For - example "title" could be the experiment_title or the - sample_title. - """ - key = "" - value = "" - - for child in attribute: - if child.tag == "TAG": - key = key_prefix + child.text.lower().replace("-", "_").replace(" ", "_") - elif child.tag == "VALUE": - value = child.text - - return (key, value) + def gather_file_report(run_accession: str): + return SraSurveyor.get_ena_json_api_response( + "filereport", + run_accession, + { + "fields": "all", + }, + ) @staticmethod - def gather_file_report(run_accession: str) -> List[Dict]: - """Get stats about files and check for unmated reads. - - This endpoint returns a weird format, so some custom parsing is required: - run_accession fastq_ftp fastq_bytes fastq_md5 submitted_ftp submitted_bytes submitted_md5 sra_ftp sra_bytes sra_md5 - SRR7353755 ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_1.fastq.gz;ftp.sra.ebi.ac.uk/vol1/fastq/SRR735/005/SRR7353755/SRR7353755_2.fastq.gz 25176;2856704;3140575 7ef1ba010dcb679217112efa380798b2;6bc5651b7103306d4d65018180ab8d0d;3856c14164612d9879d576a046a9879f - """ + def gather_experiment_biosample_metadata(biosample_accession: str): response = utils.requests_retry_session().get( - ENA_FILE_REPORT_URL_TEMPLATE.format(accession=run_accession) + f"{ENA_BIOSAMPLE_API}/{biosample_accession}?format=json" ) - lines = response.text.split("\n") - split_lines = [line.split("\t") for line in lines] - header_row = split_lines[0] - sample_row = split_lines[1] - - file_info = [] - for i, key in enumerate(header_row): - if key in ["fastq_ftp", "fastq_bytes", "fastq_md5"]: - for i, value in enumerate(sample_row[i].split(";")): - if i >= len(file_info): - file_info.append({key: value}) - else: - file_info[i][key] = value - - return file_info + if response.status_code != 200: + logger.error( + "Could not discover Biosample.", + accession=biosample_accession, + status_code=response.status_code, + url=response.url, + ) + return {} + return response.json() @staticmethod - def gather_run_metadata(run_accession: str) -> Dict: - """A run refers to a specific read in an experiment.""" - - discoverable_accessions = ["study_accession", "sample_accession", "submission_accession"] - + def gather_experiment_pubmed_metadata(accession: str): response = utils.requests_retry_session().get( - ENA_METADATA_URL_TEMPLATE.format(run_accession) + ENA_PUBMED_API_TEMPLATE.format(accession=accession) ) - try: - run_xml = ET.fromstring(response.text) - except Exception: - logger.exception("Unable to decode response", response=response.text) - return {} - - # Necessary because ERP000263 has only one ROOT element containing this error: - # Entry: ERR15562 display type is either not supported or entry is not found. - if len(run_xml) == 0: - return {} - run_item = run_xml[0] - - useful_attributes = ["center_name", "run_center", "run_date", "broker_name", "alias"] - metadata = {} - for attribute in useful_attributes: - if attribute in run_item.attrib: - metadata[attribute] = run_item.attrib[attribute] - metadata["run_accession"] = run_accession - - for child in run_item: - if child.tag == "EXPERIMENT_REF": - metadata["experiment_accession"] = child.attrib["accession"] - elif child.tag == "RUN_LINKS": - for grandchild in child: - key, value = SraSurveyor.parse_run_link(grandchild) - if value != "" and key in discoverable_accessions: - metadata[key] = value - elif child.tag == "RUN_ATTRIBUTES": - for grandchild in child: - key, value = SraSurveyor.parse_attribute(grandchild, "run_") - metadata[key] = value - - return metadata + if response.status_code != 200: + logger.error( + "Could not discover Pubmed ID.", + accession=accession, + status_code=response.status_code, + url=response.url, + ) + return [] + return response.json() @staticmethod - def gather_sample_metadata(metadata: Dict) -> None: - formatted_metadata_URL = ENA_METADATA_URL_TEMPLATE.format(metadata["sample_accession"]) - response = utils.requests_retry_session().get(formatted_metadata_URL) - sample_xml = ET.fromstring(response.text) - - sample = sample_xml[0] - - if "center_name" in sample.attrib: - metadata["sample_center_name"] = sample.attrib["center_name"] - - for child in sample: - if child.tag == "TITLE": - metadata["sample_title"] = child.text - elif child.tag == "SAMPLE_NAME": - for grandchild in child: - if grandchild.tag == "TAXON_ID": - metadata["organism_id"] = grandchild.text - elif grandchild.tag == "SCIENTIFIC_NAME": - metadata["organism_name"] = grandchild.text.upper() - elif child.tag == "SAMPLE_ATTRIBUTES": - for grandchild in child: - key, value = SraSurveyor.parse_attribute(grandchild, "sample_") - metadata[key] = value + def gather_biosample_metadata(sample_metadata): + biosample_metadata = SraSurveyor.gather_experiment_biosample_metadata( + sample_metadata["sample_accession"] + ) + biosample_metadata.pop( + "accession", None + ) # dont overwrite accession with biosample accession + biosample_metadata.pop("status", None) # these should match but biosample is all uppercase + return {**sample_metadata, **utils.flatten_dict(biosample_metadata)} @staticmethod - def gather_study_metadata(metadata: Dict) -> None: - formatted_metadata_URL = ENA_METADATA_URL_TEMPLATE.format(metadata["study_accession"]) - response = utils.requests_retry_session().get(formatted_metadata_URL) - study_xml = ET.fromstring(response.text) - - study = study_xml[0] - for child in study: - if child.tag == "DESCRIPTOR": - for grandchild in child: - # STUDY_TYPE is the only tag which uses attributes - # instead of the text for whatever reason - if grandchild.tag == "STUDY_TYPE": - metadata[grandchild.tag.lower()] = grandchild.attrib["existing_study_type"] - else: - metadata[grandchild.tag.lower()] = grandchild.text - elif child.tag == "STUDY_ATTRIBUTES": - for grandchild in child: - key, value = SraSurveyor.parse_attribute(grandchild, "study_") - metadata[key] = value - elif child.tag == "STUDY_LINKS": - for grandchild in child: - for ggc in grandchild: - if ggc.getchildren()[0].text == "pubmed": - metadata["pubmed_id"] = ggc.getchildren()[1].text - break - elif child.tag == "IDENTIFIERS": - for grandchild in child: - if ( - # Check for GEO accessions. These live inside an - # EXTERNAL_ID tag with namespace GEO - grandchild.tag == "EXTERNAL_ID" - and grandchild.attrib.get("namespace", "") == "GEO" - and re.match(r"^GSE\d{2,6}", grandchild.text) - ) or ( - # Check for ArrayExpress accessions. These live inside a - # SUBMITTER_ID tag, but the namespace is not standardized - grandchild.tag == "SUBMITTER_ID" - and re.match(r"^E-[A-Z]{4}-\d{2,6}", grandchild.text) - ): - metadata["external_id"] = grandchild.text - break + def gather_all_metadata(accession: str): + # check if experiment, if not fetch the sample, biosample and experiment + samples_metadata = SraSurveyor.gather_experiment_runs_metadata(accession) - @staticmethod - def gather_all_metadata(run_accession): - metadata = SraSurveyor.gather_run_metadata(run_accession) + if not re.match(r"^(S|E|D)RP\d+", accession): + experiment_accession = samples_metadata[0]["secondary_study_accession"] + else: + experiment_accession = accession + + experiment_metadata = SraSurveyor.gather_experiment_metadata(experiment_accession) + + if "accession" in experiment_metadata: + pubmed_metadata = SraSurveyor.gather_experiment_pubmed_metadata( + experiment_metadata["accession"] + ) + if len(pubmed_metadata) > 0: + experiment_metadata["pubmed_id"] = pubmed_metadata["Source Primary Accession"] - if metadata != {}: - SraSurveyor.gather_experiment_metadata(metadata) - SraSurveyor.gather_sample_metadata(metadata) - SraSurveyor.gather_study_metadata(metadata) - SraSurveyor.gather_submission_metadata(metadata) + for sample_metadata in samples_metadata: + sample_metadata.update(utils.flatten_dict(experiment_metadata, "study_ena")) - return metadata + return experiment_metadata, samples_metadata @staticmethod def _build_ncbi_file_url(run_accession: str): @@ -345,220 +205,186 @@ def _apply_metadata_to_experiment(experiment: Experiment, metadata: dict): experiment.source_database = "SRA" experiment.technology = "RNA-SEQ" - # We don't get this value from the API, unfortunately. - # experiment.platform_accession_code = experiment["platform_accession_code"] - - if not experiment.description: - experiment.description = "No description." - - if "study_title" in metadata: - experiment.title = metadata["study_title"] - if "study_abstract" in metadata: - experiment.description = metadata["study_abstract"] - if "lab_name" in metadata: - experiment.submitter_institution = metadata["lab_name"] - if "experiment_design_description" in metadata: - experiment.protocol_description = metadata["experiment_design_description"] - if "pubmed_id" in metadata: - experiment.pubmed_id = metadata["pubmed_id"] - experiment.has_publication = True - if "study_ena_first_public" in metadata: - experiment.source_first_published = parse_date(metadata["study_ena_first_public"]) - if "study_ena_last_update" in metadata: - experiment.source_last_modified = parse_date(metadata["study_ena_last_update"]) - - if metadata.get("external_id", None) is not None: - experiment.alternate_accession_code = metadata["external_id"] - - # Rare, but it happens. - if not experiment.protocol_description: - # metadata.get() doesn't work here because sometimes the - # key is present but its value is None, in which case None - # is returned, causing our database constraint to be - # violated. - if ( - "library_construction_protocol" in metadata - and metadata["library_construction_protocol"] - ): - experiment.protocol_description = metadata["library_construction_protocol"] - else: - experiment.protocol_description = "Protocol was never provided." - + experiment.title = metadata.get("study_title", "") + experiment.description = utils.get_nonempty( + metadata, "study_description", "No description." + ) + experiment.submitter_institution = utils.get_nonempty(metadata, "center_name", "") + experiment.source_first_published = parse_date(metadata["first_public"]) + experiment.source_last_modified = parse_date(metadata["last_updated"]) + experiment.alternate_accession_code = utils.get_nonempty(metadata, "geo_accession", None) + experiment.protocol_description = utils.get_nonempty(metadata, "protocol", []) + + # need pubmed_id + experiment.pubmed_id = metadata.get("pubmed_id", "") + experiment.has_publication = "pubmed_id" in metadata # Scrape publication title and authorship from Pubmed if experiment.pubmed_id: - pubmed_metadata = utils.get_title_and_authors_for_pubmed_id(experiment.pubmed_id) - experiment.publication_title = pubmed_metadata[0] - experiment.publication_authors = pubmed_metadata[1] + title, authors = utils.get_title_and_authors_for_pubmed_id(experiment.pubmed_id) + experiment.publication_title = title + experiment.publication_authors = authors + + @staticmethod + def get_files_urls(run_accession, is_paired): + if DOWNLOAD_SOURCE == "NCBI": + return [SraSurveyor._build_ncbi_file_url(run_accession)] + if is_paired: + return [ + _build_ena_file_url(run_accession, "_1"), + _build_ena_file_url(run_accession, "_2"), + ] + return [_build_ena_file_url(run_accession)] + + @staticmethod + def _generate_original_files(sample, sample_metadata): + is_paired = sample_metadata["library_layout"] == "PAIRED" + files_urls = SraSurveyor.get_files_urls(sample.accession_code, is_paired) + file_reports = SraSurveyor.gather_file_report(sample.accession_code) + + if len(file_reports) <= 2: + for file_url in files_urls: + original_file, _ = OriginalFile.objects.get_or_create( + source_url=file_url, source_filename=file_url.split("/")[-1], has_raw=True + ) + OriginalFileSampleAssociation.objects.get_or_create( + original_file=original_file, sample=sample + ) + else: + # If there's 3 fastq files in ENA's FTP server that's + # because one is the unmated reads and we can't + # reliably convert from the .SRA. Therefore set the + # file_urls to be the ENA FTP URLs. + for file_report in file_reports: + file_url = file_report["fastq_ftp"] + + # Skip the one for unmated reads: the unmated + # reads file lacks a _X postfix. It's be better if + # it was clearly labeled, but it seems to be + # consistent. The unmated reads file also seems to + # be the smallest by far, but I'm unsure how + # reliable that is. + if "_1.fastq.gz" in file_url or "_2.fastq.gz" in file_url: + original_file, _ = OriginalFile.objects.get_or_create( + source_url=file_url, + source_filename=file_url.split("/")[-1], + has_raw=True, + expected_size_in_bytes=file_report["fastq_bytes"], + expected_md5=file_report["fastq_md5"], + ) + OriginalFileSampleAssociation.objects.get_or_create( + original_file=original_file, sample=sample + ) def _generate_experiment_and_samples( - self, run_accession: str, study_accession: str = None + self, experiment_or_sample_accession: str ) -> (Experiment, List[Sample]): """Generates Experiments and Samples for the provided run_accession.""" - metadata = SraSurveyor.gather_all_metadata(run_accession) - - if metadata == {}: - if study_accession: - logger.error( - "Could not discover any metadata for run.", - accession=run_accession, - study_accession=study_accession, - ) - else: - logger.error("Could not discover any metadata for run.", accession=run_accession) - return (None, None) # This will cascade properly + experiment_metadata, samples_metadata = SraSurveyor.gather_all_metadata( + experiment_or_sample_accession + ) - if DOWNLOAD_SOURCE == "ENA": - if metadata["library_layout"] == "PAIRED": - files_urls = [ - _build_ena_file_url(run_accession, "_1"), - _build_ena_file_url(run_accession, "_2"), - ] - else: - files_urls = [_build_ena_file_url(run_accession)] - else: - files_urls = [SraSurveyor._build_ncbi_file_url(run_accession)] + experiment = self._generate_experment(experiment_metadata) + samples = [ + self._generate_sample(experiment, sample_metadata) + for sample_metadata in samples_metadata + ] - # Figure out the Organism for this sample - organism_name = metadata.pop("organism_name", None) - if not organism_name: - logger.error("Could not discover organism type for run.", accession=run_accession) - return (None, None) # This will cascade properly + return experiment, samples - organism_name = organism_name.upper() - organism = Organism.get_object_for_name(organism_name) + def _generate_experiment(self, experiment_metadata: dict) -> Experiment: + """Generates an Experiment for the provided experiment_accession.""" - ## - # Experiment - ## + if not experiment_metadata: + return None - experiment_accession_code = metadata.get("study_accession") - try: - experiment_object = Experiment.objects.get(accession_code=experiment_accession_code) + # secondary_study_accession is DRP, SRP etc + # study_accession is PRJDB etc + experiment_accession = experiment_metadata.get("secondary_study_accession") + + experiment, is_created = Experiment.get_or_create(accession_code=experiment_accession) + + if is_created: + SraSurveyor._apply_metadata_to_experiment(experiment, experiment_metadata) + experiment.save() + experiment_annotation = ExperimentAnnotation() + experiment_annotation.experiment = experiment + experiment_annotation.data = experiment_metadata + experiment_annotation.is_ccdl = False + experiment_annotation.save() + else: logger.debug( "Experiment already exists, skipping object creation.", - experiment_accession_code=experiment_accession_code, + experiment_accession_code=experiment_accession, survey_job=self.survey_job.id, ) - except Experiment.DoesNotExist: - experiment_object = Experiment() - experiment_object.accession_code = experiment_accession_code - SraSurveyor._apply_metadata_to_experiment(experiment_object, metadata) - experiment_object.save() - - ## - # Experiment Metadata - ## - json_xa = ExperimentAnnotation() - json_xa.experiment = experiment_object - json_xa.data = metadata - json_xa.is_ccdl = False - json_xa.save() - - ## - # Samples - ## - - sample_accession_code = metadata.pop("run_accession") - # Create the sample object - try: - sample_object = Sample.objects.get(accession_code=sample_accession_code) - # If current experiment includes new protocol information, - # merge it into the sample's existing protocol_info. - protocol_info, is_updated = self.update_sample_protocol_info( - sample_object.protocol_info, - experiment_object.protocol_description, - experiment_object.source_url, - ) - if is_updated: - sample_object.protocol_info = protocol_info - sample_object.save() - logger.debug( - "Sample %s already exists, skipping object creation.", - sample_accession_code, - experiment_accession_code=experiment_object.accession_code, - survey_job=self.survey_job.id, - ) - except Sample.DoesNotExist: - sample_object = Sample() - sample_object.source_database = "SRA" - sample_object.accession_code = sample_accession_code - sample_object.organism = organism + return experiment + + def _generate_sample(self, experiment: Experiment, sample_metadata: dict) -> Sample: + """Generates or updates a Sample for the provided Experiment and sample metadata.""" + + run_accession = sample_metadata["run_accession"] + + # Figure out the Organism for this sample + organism_name = sample_metadata.pop("scientific_name", None) + if not organism_name: + logger.error("Could not discover organism type for run.", accession=run_accession) + return (None, None) # This will cascade properly - sample_object.platform_name = metadata.get("platform_instrument_model", "UNKNOWN") + organism = Organism.get_object_for_name(organism_name) + + sample_accession = sample_metadata.pop("run_accession") + sample, sample_created = Sample.objects.get_or_create(accession_code=sample_accession) + + if sample_created: + sample.source_database = "SRA" + sample.organism = organism + sample.platform_name = sample_metadata.get("instrument_model", "UNKNOWN") # The platform_name is human readable and contains spaces, # accession codes shouldn't have spaces though: - sample_object.platform_accession_code = sample_object.platform_name.replace(" ", "") - sample_object.technology = "RNA-SEQ" - if ( - "ILLUMINA" in sample_object.platform_name.upper() - or "NEXTSEQ" in sample_object.platform_name.upper() - ): - sample_object.manufacturer = "ILLUMINA" - elif "ION TORRENT" in sample_object.platform_name.upper(): - sample_object.manufacturer = "ION_TORRENT" + sample.platform_accession_code = sample.platform_name.replace(" ", "") + sample.technology = "RNA-SEQ" + + platform_name = sample.platform_name.upper() + if platform_name in ["ILLUMINA", "NEXTSEQ"]: + sample.manufacturer = "ILLUMINA" + elif "ION TORRENT" in platform_name: + sample.manufacturer = "ION_TORRENT" else: - sample_object.manufacturer = "UNKNOWN" + sample.manufacturer = "UNKNOWN" - SraSurveyor._apply_harmonized_metadata_to_sample(sample_object, metadata) + SraSurveyor._apply_harmonized_metadata_to_sample(sample, sample_metadata) - protocol_info, is_updated = self.update_sample_protocol_info( - existing_protocols=[], - experiment_protocol=experiment_object.protocol_description, - experiment_url=experiment_object.source_url, + self._generate_original_files(sample, sample_metadata) + sample.save() + else: + logger.debug( + "Sample %s already exists, skipping object creation.", + sample_accession_code=sample_accession, + experiment_accession_code=experiment.accession_code, + survey_job=self.survey_job.id, ) - # Do not check is_updated the first time because we must - # save a list so we can append to it later. - sample_object.protocol_info = protocol_info - sample_object.save() - - file_reports = SraSurveyor.gather_file_report(sample_accession_code) + # i think we should be collecting the sample protocols + # on the experiment here instead + protocol_info, protocol_updated = self.update_sample_protocol_info( + existing_protocols=sample.protocol_info, + experiment_protocol=experiment.protocol_description, + experiment_url=experiment.source_url, + ) - if len(file_reports) < 3: - for file_url in files_urls: - original_file = OriginalFile.objects.get_or_create( - source_url=file_url, source_filename=file_url.split("/")[-1], has_raw=True - )[0] - OriginalFileSampleAssociation.objects.get_or_create( - original_file=original_file, sample=sample_object - ) - else: - # If there's 3 fastq files in ENA's FTP server that's - # because one is the unmated reads and we can't - # reliably convert from the .SRA. Therefore set the - # file_urls to be the ENA FTP URLs. - for file_report in file_reports: - file_url = file_report["fastq_ftp"] - - # Skip the one for unmated reads: the unmated - # reads file lacks a _X postfix. It's be better if - # it was clearly labeled, but it seems to be - # consistent. The unmated reads file also seems to - # be the smallest by far, but I'm unsure how - # reliable that is. - if "_1.fastq.gz" in file_url or "_2.fastq.gz" in file_url: - original_file = OriginalFile.objects.get_or_create( - source_url=file_url, - source_filename=file_url.split("/")[-1], - has_raw=True, - expected_size_in_bytes=file_report["fastq_bytes"], - expected_md5=file_report["fastq_md5"], - )[0] - OriginalFileSampleAssociation.objects.get_or_create( - original_file=original_file, sample=sample_object - ) + if sample_created or protocol_updated: + sample.protocol_info = protocol_info + sample.save() # Create associations if they don't already exist - ExperimentSampleAssociation.objects.get_or_create( - experiment=experiment_object, sample=sample_object - ) + ExperimentSampleAssociation.objects.get_or_create(experiment=experiment, sample=sample) ExperimentOrganismAssociation.objects.get_or_create( - experiment=experiment_object, organism=organism + experiment=experiment, organism=organism ) - return experiment_object, [sample_object] + return sample @staticmethod def update_sample_protocol_info(existing_protocols, experiment_protocol, experiment_url): @@ -569,16 +395,23 @@ def update_sample_protocol_info(existing_protocols, experiment_protocol, experim may or may not have been updated) and the second is a boolean indicating whether exisiting_protocols has been updated. """ + # ensure we are working with a list + # since the default value for a JSON field is an empty dict + existing_protocols_list = [] + if existing_protocols is not {}: + existing_protocols_list = existing_protocols if experiment_protocol == "Protocol was never provided.": - return (existing_protocols, False) + return (existing_protocols_list, False) - existing_descriptions = [protocol["Description"] for protocol in existing_protocols] + existing_descriptions = [protocol["Description"] for protocol in existing_protocols_list] if experiment_protocol in existing_descriptions: - return (existing_protocols, False) + return (existing_protocols_list, False) - existing_protocols.append({"Description": experiment_protocol, "Reference": experiment_url}) - return (existing_protocols, True) + existing_protocols_list.append( + {"Description": experiment_protocol, "Reference": experiment_url} + ) + return (existing_protocols_list, True) def discover_experiment_and_samples(self): """Returns an experiment and a list of samples for an SRA accession""" @@ -586,68 +419,5 @@ def discover_experiment_and_samples(self): survey_job_properties = survey_job.get_properties() accession = survey_job_properties["experiment_accession_code"] - # SRA Surveyor is mainly designed for SRRs, this handles SRPs - if "SRP" in accession or "ERP" in accession or "DRP" in accession: - response = utils.requests_retry_session().get( - ENA_METADATA_URL_TEMPLATE.format(accession) - ) - - # If the status code is 404, then SRA doesn't know about this accession - if response.status_code == 404: - return None, None - - experiment_xml = ET.fromstring(response.text)[0] - study_links = experiment_xml[2] # STUDY_LINKS - - accessions_to_run = [] - for child in study_links: - if child[0][0].text == "ENA-RUN": - all_runs = child[0][1].text - - # Ranges can be disjoint, separated by commas - run_segments = all_runs.split(",") - for segment in run_segments: - if "-" in segment: - start, end = segment.split("-") - else: - start = segment - end = segment - start_id = start[3::] - end_id = end[3::] - - for run_id in range(int(start_id), int(end_id) + 1): - run_id = str(run_id).zfill(len(start_id)) - accessions_to_run.append(accession[0] + "RR" + run_id) - break - - experiment = None - all_samples = [] - for run_id in accessions_to_run: - logger.debug( - "Surveying SRA Run Accession %s for Experiment %s", - run_id, - accession, - survey_job=self.survey_job.id, - ) - - returned_experiment, samples = self._generate_experiment_and_samples( - run_id, accession - ) - - # Some runs may return (None, None). If this happens - # we don't want to set experiment to None. - if returned_experiment: - experiment = returned_experiment - - if samples: - all_samples += samples - - # So we prevent duplicate downloads, ex for SRP111553 - all_samples = list(set(all_samples)) - - # Experiment will always be the same - return experiment, all_samples - - else: - logger.debug("Surveying SRA Run Accession %s", accession, survey_job=self.survey_job.id) - return self._generate_experiment_and_samples(accession) + logger.debug("Surveying SRA Accession %s", accession, survey_job=self.survey_job.id) + return self._generate_experiment_and_samples(accession) From bd4396455354baad89ee741c305c8766da73acc5 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 14 Jul 2023 14:52:19 -0400 Subject: [PATCH 163/220] update tests for new ENA response --- foreman/tests/surveyor/test_sra.py | 115 +++++++++++++++-------------- 1 file changed, 58 insertions(+), 57 deletions(-) diff --git a/foreman/tests/surveyor/test_sra.py b/foreman/tests/surveyor/test_sra.py index 4a6a89234..15da5ddbc 100644 --- a/foreman/tests/surveyor/test_sra.py +++ b/foreman/tests/surveyor/test_sra.py @@ -184,78 +184,66 @@ def test_arrayexpress_alternate_accession(self): ) def test_metadata_is_gathered_correctly(self): - metadata = SraSurveyor.gather_all_metadata("DRR002116") + _, samples_metadata = SraSurveyor.gather_all_metadata("DRR002116") - self.assertEqual(metadata["broker_name"], "DDBJ") - self.assertEqual(metadata["center_name"], "RIKEN_CDB") - self.assertEqual(metadata["ena-base-count"], "158881910957") - self.assertEqual(metadata["ena-spot-count"], "1371813555") - self.assertEqual(metadata["experiment_accession"], "DRX001563") - self.assertEqual( - metadata["experiment_design_description"], - ("Experiment for mRNAseq of chicken at stage " "HH16 (biological replicate 1)"), - ) + sample_metadata = samples_metadata[0] + self.assertEqual(sample_metadata["broker_name"], "") # used to be DDBJ + self.assertEqual(sample_metadata["center_name"], "RIKEN_CDB") + self.assertEqual(sample_metadata["experiment_accession"], "DRX001563") self.assertEqual( - metadata["experiment_title"], - ("Illumina HiSeq 2000 sequencing; " "Exp_Gg_HH16_1_embryo_mRNAseq"), + sample_metadata["characteristics_sample_comment_0_text"], + "mRNAseq of chicken at stage HH16 (biological replicate 1)", ) self.assertEqual( - metadata["lab_name"], + sample_metadata["experiment_title"], ( - "Group for Morphological Evolution, Center for Developmental " - "Biology, Kobe Institute, RIKEN" + "Illumina HiSeq 2000 sequencing; " + "Exp_Gg_HH16_1_embryo_mRNAseq;" + "Exp_Gg_HH16_1_embryo_mRNAseq" ), ) - self.assertEqual(metadata["library_layout"], "SINGLE") - self.assertEqual(metadata["library_name"], "Gg_HH16_1_embryo_mRNAseq") - self.assertEqual(metadata["library_selection"], "RANDOM") - self.assertEqual(metadata["library_source"], "TRANSCRIPTOMIC") - self.assertEqual(metadata["library_strategy"], "RNA-Seq") - self.assertEqual(metadata["organism_id"], "9031") - self.assertEqual(metadata["organism_name"], "GALLUS GALLUS") - self.assertEqual(metadata["platform_instrument_model"], "Illumina HiSeq 2000") - self.assertEqual(metadata["read_spec_0_base_coord"], "1") - self.assertEqual(metadata["read_spec_0_class"], "Application Read") - self.assertEqual(metadata["read_spec_0_index"], "0") - self.assertEqual(metadata["read_spec_0_type"], "Forward") - self.assertEqual(metadata["run_accession"], "DRR002116") - self.assertEqual(metadata["run_center"], "RIKEN_CDB") - self.assertEqual(metadata["run_date"], "2011-09-01T00:00:00+09:00") - self.assertEqual(metadata["run_ena_base_count"], "3256836000") - self.assertEqual(metadata["run_ena_first_public"], "2013-07-19") - self.assertEqual(metadata["run_ena_last_update"], "2017-08-11") - self.assertEqual(metadata["run_ena_spot_count"], "32568360") - self.assertEqual(metadata["sample_accession"], "DRS001521") self.assertEqual( - metadata["sample_center_name"], + sample_metadata["study_ena_center_name"], + "RIKEN Center for Developmental Biology", + ) + self.assertEqual(sample_metadata["library_layout"], "SINGLE") + self.assertEqual(sample_metadata["library_name"], "Gg_HH16_1_embryo_mRNAseq") + self.assertEqual(sample_metadata["library_selection"], "RANDOM") + self.assertEqual(sample_metadata["library_source"], "TRANSCRIPTOMIC") + self.assertEqual(sample_metadata["library_strategy"], "RNA-Seq") + self.assertEqual(sample_metadata["tax_id"], "9031") # aka organism_id + self.assertEqual(sample_metadata["scientific_name"], "Gallus gallus") # aka organism_name + self.assertEqual( + sample_metadata["instrument_model"], "Illumina HiSeq 2000" + ) # aka platform_instrument_model + self.assertEqual(sample_metadata["run_accession"], "DRR002116") + self.assertEqual(sample_metadata["run_date"], "1314831600000") + self.assertEqual(sample_metadata["base_count"], "3256836000") + self.assertEqual(sample_metadata["first_public"], "2013-07-19") + self.assertEqual(sample_metadata["last_updated"], "2017-08-11") + self.assertEqual(sample_metadata["read_count"], "32568360") + self.assertEqual(sample_metadata["secondary_sample_accession"], "DRS001521") + self.assertEqual( + sample_metadata["characteristics_INSDC_center_name_0_text"], "Group for Morphological Evolution, Center for Developmental Biology, Kobe Institute, RIKEN", ) - self.assertEqual(metadata["sample_ena_first_public"], "2013-02-27") - self.assertEqual(metadata["sample_ena_last_update"], "2014-11-12") self.assertEqual( - metadata["sample_sample_comment"], - ("mRNAseq of chicken at stage HH16 (biological " "replicate 1)"), + sample_metadata["characteristics_INSDC_first_public_0_text"], "2013-02-27T00:00:00Z" ) - self.assertEqual(metadata["sample_sample_name"], "DRS001521") - self.assertEqual(metadata["sample_title"], "Gg_HH16_1_embryo_mRNAseq") - self.assertEqual(metadata["spot_length"], "100") - self.assertEqual(metadata["study_ena_first_public"], "2013-07-19") - self.assertEqual(metadata["study_ena_last_update"], "2015-06-22") - self.assertEqual(metadata["study_accession"], "DRP000595") - self.assertEqual(metadata["submission_accession"], "DRA000567") self.assertEqual( - metadata["submission_comment"], - ( - "Time course gene expression profiles of turtle " - "(Pelodiscus sinensis) and chicken (Gallus gallus) " - "embryos were examined. Whole transcriptome of turtle " - "was also determined by uding stranded sequencing " - "methods." - ), + sample_metadata["characteristics_sample_comment_0_text"], + "mRNAseq of chicken at stage HH16 (biological replicate 1)", + ) + self.assertEqual(sample_metadata["secondary_sample_accession"], "DRS001521") + self.assertEqual( + sample_metadata["characteristics_title_0_text"], "Gg_HH16_1_embryo_mRNAseq" ) - self.assertEqual(metadata["submission_title"], "Submitted by RIKEN_CDB on 19-JUL-2013") + self.assertEqual(sample_metadata["study_ena_first_public"], "2013-12-14") + self.assertEqual(sample_metadata["study_ena_last_updated"], "2023-05-19") + self.assertEqual(sample_metadata["secondary_study_accession"], "DRP000595") + self.assertEqual(sample_metadata["submission_accession"], "DRA000567") - ncbi_url = SraSurveyor._build_ncbi_file_url(metadata["run_accession"]) + ncbi_url = SraSurveyor._build_ncbi_file_url(sample_metadata["run_accession"]) self.assertEqual( ncbi_url, "https://sra-pub-run-odp.s3.amazonaws.com/sra/DRR002116/DRR002116" ) @@ -279,3 +267,16 @@ def test_sra_metadata_is_harmonized(self): ) self.assertEqual(experiment.source_first_published, datetime.date(2017, 8, 25)) self.assertEqual(experiment.source_last_modified, datetime.date(2023, 5, 19)) + + def test_sra_ena_api_endpoint_construction(self): + expected_filereport_url = "https://www.ebi.ac.uk/ena/portal/api/filereport/?format=json&accession=SRP047410&result=read_run" + filereport_url = SraSurveyor.get_ena_json_api( + "filereport", "SRP047410", {"result": "read_run"} + ) + self.assertEqual(expected_filereport_url, filereport_url) + + expected_metadata_url = ( + "https://www.ebi.ac.uk/ena/portal/api/search/?format=json&accession=SRP047410" + ) + metadata_url = SraSurveyor.get_ena_json_api("search", "SRP047410", {}) + self.assertEquual(expected_metadata_url, metadata_url) From e31aed0fb5463d5634378c514511417bc123d2c0 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 14 Jul 2023 15:12:23 -0400 Subject: [PATCH 164/220] key_strings as set --- foreman/data_refinery_foreman/surveyor/harmony.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index f706df68e..7cf178fcc 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -768,7 +768,7 @@ def harmonize_field( harmonized_values = set() key_variants = getattr(self, variants_attribute) key_regexes = [r for r in key_variants if isinstance(r, RE_TYPE)] - key_strings = [s for s in key_variants if isinstance(s, str)] + key_strings = set([s for s in key_variants if isinstance(s, str)]) for key, value in sample_metadata.items(): lower_key = key.lower().strip() From e9876b2aabd14a2fa1b53955649b839f6245671b Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 17 Jul 2023 10:27:07 -0400 Subject: [PATCH 165/220] add test for pubmed metadata --- foreman/data_refinery_foreman/surveyor/sra.py | 3 +- foreman/tests/surveyor/test_sra.py | 30 +++++++++++++++++++ 2 files changed, 31 insertions(+), 2 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index bff26a741..9491a4999 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -179,7 +179,7 @@ def gather_all_metadata(accession: str): experiment_metadata["accession"] ) if len(pubmed_metadata) > 0: - experiment_metadata["pubmed_id"] = pubmed_metadata["Source Primary Accession"] + experiment_metadata["pubmed_id"] = pubmed_metadata[0]["Source Primary Accession"] for sample_metadata in samples_metadata: sample_metadata.update(utils.flatten_dict(experiment_metadata, "study_ena")) @@ -215,7 +215,6 @@ def _apply_metadata_to_experiment(experiment: Experiment, metadata: dict): experiment.alternate_accession_code = utils.get_nonempty(metadata, "geo_accession", None) experiment.protocol_description = utils.get_nonempty(metadata, "protocol", []) - # need pubmed_id experiment.pubmed_id = metadata.get("pubmed_id", "") experiment.has_publication = "pubmed_id" in metadata # Scrape publication title and authorship from Pubmed diff --git a/foreman/tests/surveyor/test_sra.py b/foreman/tests/surveyor/test_sra.py index 15da5ddbc..b177553db 100644 --- a/foreman/tests/surveyor/test_sra.py +++ b/foreman/tests/surveyor/test_sra.py @@ -268,6 +268,36 @@ def test_sra_metadata_is_harmonized(self): self.assertEqual(experiment.source_first_published, datetime.date(2017, 8, 25)) self.assertEqual(experiment.source_last_modified, datetime.date(2023, 5, 19)) + def test_sra_pubmed_metdata_is_applied(self): + experiment_metadata, _ = SraSurveyor.gather_all_metadata("SRR3098582") + + experiment = Experiment() + SraSurveyor._apply_metadata_to_experiment(experiment, experiment_metadata) + self.assertEqual(experiment.pubmed_id, "27102575") + self.assertEqual(experiment.has_publication, True) + self.assertEqual( + experiment.publication_title, + "Glutathione antioxidant pathway activity and reserve determine toxicity and specificity of the biliary toxin biliatresone in zebrafish.", + ) + self.assertEqual( + experiment.publication_authors, + [ + "Zhao X", + "Lorent K", + "Wilkins BJ", + "Marchione DM", + "Gillespie K", + "Waisbourd-Zinman O", + "So J", + "Koo KA", + "Shin D", + "Porter JR", + "Wells RG", + "Blair I", + "Pack M", + ], + ) + def test_sra_ena_api_endpoint_construction(self): expected_filereport_url = "https://www.ebi.ac.uk/ena/portal/api/filereport/?format=json&accession=SRP047410&result=read_run" filereport_url = SraSurveyor.get_ena_json_api( From 6a28c93498251d41e60fab0ac842df7ca361fe79 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 17 Jul 2023 10:31:07 -0400 Subject: [PATCH 166/220] set comprehension notation --- foreman/data_refinery_foreman/surveyor/harmony.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 7cf178fcc..d1194f1f3 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -768,7 +768,7 @@ def harmonize_field( harmonized_values = set() key_variants = getattr(self, variants_attribute) key_regexes = [r for r in key_variants if isinstance(r, RE_TYPE)] - key_strings = set([s for s in key_variants if isinstance(s, str)]) + key_strings = {s for s in key_variants if isinstance(s, str)} for key, value in sample_metadata.items(): lower_key = key.lower().strip() From b2363f1f5923c9e5cac63238576c212ac5a086e0 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 17 Jul 2023 10:48:29 -0400 Subject: [PATCH 167/220] fix typo --- foreman/data_refinery_foreman/surveyor/harmony.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index f706df68e..f22496acb 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -570,7 +570,7 @@ def __init__(self): "sex", "gender", "subject gender", - "subjext sex", + "subject sex", # This looks reduntant, but there are some samples which use # Characteristic[Characteristic[sex]] "characteristic [sex]", From f6847e681cac2874d17f63182aab393a07e67f63 Mon Sep 17 00:00:00 2001 From: David Date: Mon, 17 Jul 2023 10:52:13 -0400 Subject: [PATCH 168/220] type hint accession Co-authored-by: Joshua Shapiro --- foreman/data_refinery_foreman/surveyor/sra.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index 9491a4999..c4e20b690 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -54,7 +54,7 @@ def source_type(self): return Downloaders.SRA.value @staticmethod - def get_ena_json_api(endpoint: str, accession, params: dict = {}): + def get_ena_json_api(endpoint: str, accession: str, params: dict = {}): query_params = "&".join([f"{k}={params[k]}" for k in params]) if query_params: From d7abfbddd45a96091844252b98adbc0b7c20aea3 Mon Sep 17 00:00:00 2001 From: David Date: Mon, 17 Jul 2023 10:52:37 -0400 Subject: [PATCH 169/220] type hint separator Co-authored-by: Joshua Shapiro --- foreman/data_refinery_foreman/surveyor/utils.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/utils.py b/foreman/data_refinery_foreman/surveyor/utils.py index 7992dac33..33b47efcf 100644 --- a/foreman/data_refinery_foreman/surveyor/utils.py +++ b/foreman/data_refinery_foreman/surveyor/utils.py @@ -46,7 +46,7 @@ def flatten(d, parent_key="", sep="_"): # TODO: Merge this with above method after updating array express # to support recursive flattening. -def flatten_dict(data: Union[Dict, List], prefix: str = "", sep="_") -> dict: +def flatten_dict(data: Union[Dict, List], prefix: str = "", sep: str = "_") -> dict: """Flattens a nested dictionary or list""" flattened = {} iterator = enumerate(data) if isinstance(data, list) else data.items() From cf7afca49a8c5fe2d8c53e565f978ca77e22fec6 Mon Sep 17 00:00:00 2001 From: David Date: Mon, 17 Jul 2023 11:08:40 -0400 Subject: [PATCH 170/220] pass multiple types to check Co-authored-by: Arkadii Yakovets --- foreman/data_refinery_foreman/surveyor/utils.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/utils.py b/foreman/data_refinery_foreman/surveyor/utils.py index 33b47efcf..7fcd8f025 100644 --- a/foreman/data_refinery_foreman/surveyor/utils.py +++ b/foreman/data_refinery_foreman/surveyor/utils.py @@ -53,7 +53,7 @@ def flatten_dict(data: Union[Dict, List], prefix: str = "", sep: str = "_") -> d for k, v in iterator: key = (k if not prefix else f"{prefix}{sep}{k}").replace(" ", sep) - if isinstance(v, dict) or isinstance(v, list): + if isinstance(v, (dict, list)): flattened.update(flatten_dict(v, key)) else: flattened[key] = v From 34f6c27392a329203b3ba0b940c87ee6769b09d3 Mon Sep 17 00:00:00 2001 From: David Date: Mon, 17 Jul 2023 11:09:24 -0400 Subject: [PATCH 171/220] use short circuit Co-authored-by: Arkadii Yakovets --- foreman/data_refinery_foreman/surveyor/utils.py | 4 +--- 1 file changed, 1 insertion(+), 3 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/utils.py b/foreman/data_refinery_foreman/surveyor/utils.py index 7fcd8f025..5d7b1ec2b 100644 --- a/foreman/data_refinery_foreman/surveyor/utils.py +++ b/foreman/data_refinery_foreman/surveyor/utils.py @@ -62,9 +62,7 @@ def flatten_dict(data: Union[Dict, List], prefix: str = "", sep: str = "_") -> d def get_nonempty(hash: dict, key: str, fallback): - if key in hash and hash[key]: - return hash[key] - return fallback + return hash.get(key) or fallback def get_title_and_authors_for_pubmed_id(pmid): From 2ab11ff39a24ad40aa6206f6cf62f7b1b4d822df Mon Sep 17 00:00:00 2001 From: David Date: Mon, 17 Jul 2023 14:50:55 -0400 Subject: [PATCH 172/220] fstring interpolation Co-authored-by: Joshua Shapiro --- foreman/data_refinery_foreman/surveyor/sra.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index c4e20b690..8a3181e7a 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -70,7 +70,7 @@ def get_ena_json_api_response(endpoint: str, accession, params: dict = {}): # handle logging of errors here consistently if response.status_code != 200: logger.error( - "Could not discover {}.".format(endpoint), + f"Could not discover {endpoint}.", accession=accession, status_code=response.status_code, url=response.url, From bd7be129d4d2eb30203f12c170e25b73818265bb Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 17 Jul 2023 14:52:38 -0400 Subject: [PATCH 173/220] instrument_platform is manufacturer --- foreman/data_refinery_foreman/surveyor/sra.py | 12 ++---------- 1 file changed, 2 insertions(+), 10 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index c4e20b690..c6c06058f 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -338,23 +338,15 @@ def _generate_sample(self, experiment: Experiment, sample_metadata: dict) -> Sam if sample_created: sample.source_database = "SRA" sample.organism = organism + sample.manufacturer = sample_metadata.get("instrument_platform", "UNKNOWN") sample.platform_name = sample_metadata.get("instrument_model", "UNKNOWN") - # The platform_name is human readable and contains spaces, - # accession codes shouldn't have spaces though: sample.platform_accession_code = sample.platform_name.replace(" ", "") sample.technology = "RNA-SEQ" - platform_name = sample.platform_name.upper() - if platform_name in ["ILLUMINA", "NEXTSEQ"]: - sample.manufacturer = "ILLUMINA" - elif "ION TORRENT" in platform_name: - sample.manufacturer = "ION_TORRENT" - else: - sample.manufacturer = "UNKNOWN" - SraSurveyor._apply_harmonized_metadata_to_sample(sample, sample_metadata) self._generate_original_files(sample, sample_metadata) + sample.save() else: logger.debug( From 6c4f92a0ec3d6feb608af72bf06949bd2a717df3 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 17 Jul 2023 15:49:49 -0400 Subject: [PATCH 174/220] add edge case check back --- foreman/data_refinery_foreman/surveyor/sra.py | 7 +++++++ 1 file changed, 7 insertions(+) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index 2bd22a133..379b1e464 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -343,6 +343,13 @@ def _generate_sample(self, experiment: Experiment, sample_metadata: dict) -> Sam sample.platform_accession_code = sample.platform_name.replace(" ", "") sample.technology = "RNA-SEQ" + # In the rare case that we get something we don't expect + # we don't want to process this sample so we can set it to UNKNOWN. + if sample.manufacturer not in ["ILLUMINA", "ION_TORRENT"]: + sample.manufacturer = "UNKNOWN" + sample.platform_name = "UNKNOWN" + sample.platform_accession_code = "UNKNOWN" + SraSurveyor._apply_harmonized_metadata_to_sample(sample, sample_metadata) self._generate_original_files(sample, sample_metadata) From 36efa6bab21dbffcaf15366517f62169adb954b2 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 17 Jul 2023 14:52:54 -0700 Subject: [PATCH 175/220] Update tests running scripts - Use versioned docker images - Exclude tests from coverage run --- .github/workflows/config.yml | 70 -------------------- api/dockerfiles/Dockerfile.api_base | 1 + api/run_tests.sh | 3 +- common/dockerfiles/Dockerfile.base | 3 + common/dockerfiles/Dockerfile.common_tests | 1 - common/dockerfiles/Dockerfile.migrations | 1 - common/run_tests.sh | 3 +- foreman/dockerfiles/Dockerfile.foreman | 1 - foreman/run_tests.sh | 3 +- scripts/common.sh | 15 +++-- scripts/prepare_image.sh | 10 ++- setup.cfg | 11 +-- workers/dockerfiles/Dockerfile.affymetrix | 1 - workers/dockerfiles/Dockerfile.compendia | 1 + workers/dockerfiles/Dockerfile.downloaders | 1 - workers/dockerfiles/Dockerfile.illumina | 1 - workers/dockerfiles/Dockerfile.no_op | 1 - workers/dockerfiles/Dockerfile.salmon | 1 - workers/dockerfiles/Dockerfile.smasher | 1 - workers/dockerfiles/Dockerfile.transcriptome | 1 - workers/run_tests.sh | 4 +- 21 files changed, 35 insertions(+), 99 deletions(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 902cd0765..11197af48 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -197,16 +197,6 @@ jobs: - name: Build the Docker Image run: scripts/prepare_image.sh -i base -s common - - name: Login to Packages Container Registry - uses: docker/login-action@v2 - with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Push Built Docker Images - run: .github/scripts/push_docker_images.sh - test_common: name: Test Common env: @@ -248,16 +238,6 @@ jobs: common/run_tests.sh .github/scripts/upload_test_coverage.sh common - - name: Login to Packages Container Registry - uses: docker/login-action@v2 - with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Push Built Docker Images - run: .github/scripts/push_docker_images.sh - test_downloaders_no_op: name: Test Downloaders and No-Op env: @@ -336,16 +316,6 @@ jobs: foreman/run_tests.sh --exclude-tag=end_to_end .github/scripts/upload_test_coverage.sh foreman - - name: Login to Packages Container Registry - uses: docker/login-action@v2 - with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Push Built Docker Images - run: .github/scripts/push_docker_images.sh - test_illumina: name: Test Illumina env: @@ -381,16 +351,6 @@ jobs: .github/scripts/filter_tests.sh -t illumina .github/scripts/upload_test_coverage.sh workers - - name: Login to Packages Container Registry - uses: docker/login-action@v2 - with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Push Built Docker Images - run: .github/scripts/push_docker_images.sh - test_salmon: name: Test Salmon env: @@ -425,16 +385,6 @@ jobs: .github/scripts/filter_tests.sh -t salmon .github/scripts/upload_test_coverage.sh workers - - name: Login to Packages Container Registry - uses: docker/login-action@v2 - with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Push Built Docker Images - run: .github/scripts/push_docker_images.sh - test_smasher: name: Test Smasher env: @@ -488,16 +438,6 @@ jobs: .github/scripts/filter_tests.sh -t qn .github/scripts/upload_test_coverage.sh workers - - name: Login to Packages Container Registry - uses: docker/login-action@v2 - with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Push Built Docker Images - run: .github/scripts/push_docker_images.sh - test_transcriptome: name: Test Transcriptome env: @@ -534,16 +474,6 @@ jobs: .github/scripts/filter_tests.sh -t transcriptome .github/scripts/upload_test_coverage.sh workers - - name: Login to Packages Container Registry - uses: docker/login-action@v2 - with: - registry: ghcr.io - username: ${{ github.actor }} - password: ${{ secrets.GITHUB_TOKEN }} - - - name: Push Built Docker Images - run: .github/scripts/push_docker_images.sh - # Deploy jobs. deploy: name: Deploy diff --git a/api/dockerfiles/Dockerfile.api_base b/api/dockerfiles/Dockerfile.api_base index d1d751104..44bf4c787 100644 --- a/api/dockerfiles/Dockerfile.api_base +++ b/api/dockerfiles/Dockerfile.api_base @@ -23,6 +23,7 @@ RUN pip install --ignore-installed \ COPY api/ . COPY config/ config/ +COPY setup.cfg . ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/api/run_tests.sh b/api/run_tests.sh index f4353c679..0b4072131 100755 --- a/api/run_tests.sh +++ b/api/run_tests.sh @@ -35,6 +35,7 @@ if [ ! -d "$volume_directory" ]; then fi chmod -R a+rwX "$volume_directory" +./scripts/prepare_image.sh -i api_base -s api ./scripts/prepare_image.sh -i api_local -s api . ./scripts/common.sh @@ -56,5 +57,5 @@ docker run \ --tty \ --volume "$volume_directory":/home/user/data_store \ $INTERACTIVE \ - "$DOCKERHUB_REPO/dr_api_local" \ + "$DOCKERHUB_REPO/dr_api_local:$SYSTEM_VERSION" \ bash -c "$(run_tests_with_coverage "$@")" diff --git a/common/dockerfiles/Dockerfile.base b/common/dockerfiles/Dockerfile.base index 13987e894..ef5d13c3b 100644 --- a/common/dockerfiles/Dockerfile.base +++ b/common/dockerfiles/Dockerfile.base @@ -66,4 +66,7 @@ RUN apt-get update && \ ENV R_LIBS=/usr/local/lib/R/site-library +COPY config/ config/ +COPY setup.cfg . + ENTRYPOINT [] diff --git a/common/dockerfiles/Dockerfile.common_tests b/common/dockerfiles/Dockerfile.common_tests index 26b2be946..d914a52f0 100644 --- a/common/dockerfiles/Dockerfile.common_tests +++ b/common/dockerfiles/Dockerfile.common_tests @@ -15,7 +15,6 @@ COPY common/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt COPY common/ . -COPY config config ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/common/dockerfiles/Dockerfile.migrations b/common/dockerfiles/Dockerfile.migrations index da2405265..516ce642b 100644 --- a/common/dockerfiles/Dockerfile.migrations +++ b/common/dockerfiles/Dockerfile.migrations @@ -11,7 +11,6 @@ COPY common/requirements.txt . RUN pip install --ignore-installed --no-cache-dir -r requirements.txt COPY common/ . -COPY config config ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/common/run_tests.sh b/common/run_tests.sh index 41eb8e3d1..25af0edb3 100755 --- a/common/run_tests.sh +++ b/common/run_tests.sh @@ -28,6 +28,7 @@ if [ ! -d "$volume_directory" ]; then fi chmod -R a+rwX "$volume_directory" +./scripts/prepare_image.sh -i base -s common ./scripts/prepare_image.sh -i common_tests -s common . ./scripts/common.sh @@ -49,5 +50,5 @@ docker run \ --tty \ --volume "$volume_directory":/home/user/data_store \ $INTERACTIVE \ - "$DOCKERHUB_REPO/dr_common_tests" \ + "$DOCKERHUB_REPO/dr_common_tests:$SYSTEM_VERSION" \ bash -c "$(run_tests_with_coverage "$@")" --parallel diff --git a/foreman/dockerfiles/Dockerfile.foreman b/foreman/dockerfiles/Dockerfile.foreman index 89e3611f0..026f5159f 100644 --- a/foreman/dockerfiles/Dockerfile.foreman +++ b/foreman/dockerfiles/Dockerfile.foreman @@ -21,7 +21,6 @@ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto -COPY config config COPY foreman/ . ENV SYSTEM_VERSION=$SYSTEM_VERSION diff --git a/foreman/run_tests.sh b/foreman/run_tests.sh index f0ae37de4..7ec565510 100755 --- a/foreman/run_tests.sh +++ b/foreman/run_tests.sh @@ -32,6 +32,7 @@ if ! [ "$(docker ps --filter name=drdb -q)" ]; then exit 1 fi +./scripts/prepare_image.sh -i base -s common ./scripts/prepare_image.sh -i foreman -s foreman . ./scripts/common.sh @@ -51,5 +52,5 @@ docker run \ --tty \ --volume "$volume_directory":/home/user/data_store \ $INTERACTIVE \ - "$DOCKERHUB_REPO/dr_foreman" \ + "$DOCKERHUB_REPO/dr_foreman:$SYSTEM_VERSION" \ bash -c "$(run_tests_with_coverage --exclude-tag=manual "$@")" diff --git a/scripts/common.sh b/scripts/common.sh index b30416abc..f995b801a 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -6,11 +6,6 @@ export ALL_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no # Sometimes we only want to work with the worker images. export WORKER_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders" -# Default Docker registry. -if [ -z "$DOCKERHUB_REPO" ]; then - export DOCKERHUB_REPO="ccdlstaging" -fi - # This function checks whether a given docker image name ($1:$2) # exists in Docker Hub or not using Docker Hub API V2. Based on: # https://stackoverflow.com/questions/32113330/check-if-imagetag-combination-already-exists-on-docker-hub @@ -118,3 +113,13 @@ EOF echo "Using Docker builder $DOCKER_BUILDER." docker buildx use "$DOCKER_BUILDER" } + +# Default Docker registry. +if [ -z "$DOCKERHUB_REPO" ]; then + export DOCKERHUB_REPO="ccdlstaging" +fi + +# Defaults to commit hash value for if we're not running in the cloud. +if [ -z "$SYSTEM_VERSION" ]; then + SYSTEM_VERSION="$(get_branch_hash)" +fi diff --git a/scripts/prepare_image.sh b/scripts/prepare_image.sh index d9a49c68d..4aee4b79e 100755 --- a/scripts/prepare_image.sh +++ b/scripts/prepare_image.sh @@ -86,7 +86,7 @@ if [ -z "$SYSTEM_VERSION" ]; then fi if [ -z "$DOCKER_ACTION" ]; then - DOCKER_ACTION="--load" + DOCKER_ACTION="--push" fi DOCKERHUB_IMAGE="$DOCKERHUB_REPO/dr_$IMAGE_NAME" @@ -141,8 +141,6 @@ if [ $finished -ne 0 ] && [ $attempt -ge $attempts ]; then exit 1 fi -if test "$GITHUB_ACTION"; then - docker pull \ - --platform linux/amd64 \ - "$DOCKERHUB_IMAGE" -fi +docker pull \ + --platform linux/amd64 \ + "$DOCKERHUB_IMAGE:$SYSTEM_VERSION" diff --git a/setup.cfg b/setup.cfg index 8b9a8a747..a4b2eb30b 100644 --- a/setup.cfg +++ b/setup.cfg @@ -1,8 +1,11 @@ -[coverage:report] -include = - data_refinery_api/* +[coverage:run] omit = - data_refinery_api/wsgi.py + tests/* + manage.py + settings.py + setup.py + urls.py + wsgi.py [pep8] max-line-length = 100 diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index 8dad322b3..69983d365 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -23,7 +23,6 @@ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto -COPY config/ config/ COPY workers/ . RUN rm -rf /root/.cache/* diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 371abf2c7..23957c892 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -92,6 +92,7 @@ RUN pip3 install --ignore-installed --no-cache-dir \ COPY .boto .boto COPY config/ config/ +COPY setup.cfg . COPY workers/ . RUN rm -rf /root/.cache/* diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 5361db49f..284203b35 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -31,7 +31,6 @@ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto -COPY config config COPY workers/ . RUN rm -rf /root/.cache/* diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index 1d771b322..ae00c06ca 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -20,7 +20,6 @@ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto -COPY config/ config/ COPY workers/ . COPY workers/data_refinery_workers/processors/detect_database.R . COPY workers/illumina_probe_maps/ probe_maps/ diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index 7e33a14e3..f37a21ce4 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -32,7 +32,6 @@ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto -COPY config/ config/ COPY workers/ . RUN rm -rf /root/.cache/* diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index 08a3d3299..6fb5885cb 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -56,7 +56,6 @@ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto -COPY config/ config/ COPY workers/ . RUN rm -rf /root/.cache/* diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index f95da5016..e04410251 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -20,7 +20,6 @@ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto -COPY config/ config/ COPY workers/ . RUN rm -rf /root/.cache/* diff --git a/workers/dockerfiles/Dockerfile.transcriptome b/workers/dockerfiles/Dockerfile.transcriptome index e3c5328a4..a27cc15aa 100644 --- a/workers/dockerfiles/Dockerfile.transcriptome +++ b/workers/dockerfiles/Dockerfile.transcriptome @@ -38,7 +38,6 @@ RUN pip3 install --ignore-installed --no-cache-dir \ common/$(ls common -1 | sort --version-sort | tail -1) COPY .boto .boto -COPY config/ config/ COPY workers/ . RUN rm -rf /root/.cache/* diff --git a/workers/run_tests.sh b/workers/run_tests.sh index a66d0a2f7..092f189bc 100755 --- a/workers/run_tests.sh +++ b/workers/run_tests.sh @@ -529,6 +529,8 @@ fi . ./scripts/common.sh +./scripts/prepare_image.sh -i base -s common + DB_HOST_IP=$(get_docker_db_ip_address) # Ensure permissions are set for everything within the test data directory. @@ -577,7 +579,7 @@ for image in $worker_images; do --tty \ --volume "$volume_directory":/home/user/data_store \ $INTERACTIVE \ - "$image_name" \ + "$image_name:$SYSTEM_VERSION" \ bash -c "$test_command" fi done From 9574462d82b9938b9345a4ccceff8af3b79101fb Mon Sep 17 00:00:00 2001 From: David Mejia Date: Tue, 18 Jul 2023 09:48:04 -0400 Subject: [PATCH 176/220] log when encountering unknown manufacturer --- foreman/data_refinery_foreman/surveyor/sra.py | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index 379b1e464..01a1fde78 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -349,6 +349,12 @@ def _generate_sample(self, experiment: Experiment, sample_metadata: dict) -> Sam sample.manufacturer = "UNKNOWN" sample.platform_name = "UNKNOWN" sample.platform_accession_code = "UNKNOWN" + logger.debug( + "Sample %s has unrecognized manufacturer.", + sample_accession_code=sample_accession, + experiment_accession_code=experiment.accession_code, + survey_job=self.survey_job.id, + ) SraSurveyor._apply_harmonized_metadata_to_sample(sample, sample_metadata) From 9409d2d471fcc9b0d2ff36385eedfe99461e6ffc Mon Sep 17 00:00:00 2001 From: David Mejia Date: Tue, 18 Jul 2023 12:30:38 -0400 Subject: [PATCH 177/220] gather metadata returns tuple --- .../management/commands/update_experiment_metadata.py | 7 +++---- 1 file changed, 3 insertions(+), 4 deletions(-) diff --git a/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py b/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py index 21ef3fe11..9e6696435 100644 --- a/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py +++ b/foreman/data_refinery_foreman/foreman/management/commands/update_experiment_metadata.py @@ -8,8 +8,7 @@ from data_refinery_common.logging import get_and_configure_logger from data_refinery_common.models import Experiment from data_refinery_common.performant_pagination.pagination import PerformantPaginator -from data_refinery_foreman.surveyor import utils -from data_refinery_foreman.surveyor.array_express import EXPERIMENTS_URL, ArrayExpressSurveyor +from data_refinery_foreman.surveyor.array_express import ArrayExpressSurveyor from data_refinery_foreman.surveyor.geo import GeoSurveyor from data_refinery_foreman.surveyor.sra import SraSurveyor @@ -56,10 +55,10 @@ def handle(self, *args, **options): ) try: if experiment.source_database == "SRA": - metadata = SraSurveyor.gather_all_metadata( + experiment_metadata, sample_metadata = SraSurveyor.gather_all_metadata( experiment.samples.first().accession_code ) - SraSurveyor._apply_metadata_to_experiment(experiment, metadata) + SraSurveyor._apply_metadata_to_experiment(experiment, experiment_metadata) elif experiment.source_database == "GEO": gse = GEOparse.get_GEO( From cb2f48b781fd2e7b3818647a0529ade87970191b Mon Sep 17 00:00:00 2001 From: David Mejia Date: Tue, 18 Jul 2023 12:31:38 -0400 Subject: [PATCH 178/220] use urlencode --- foreman/data_refinery_foreman/surveyor/sra.py | 9 ++++----- foreman/tests/surveyor/test_sra.py | 7 ++++--- 2 files changed, 8 insertions(+), 8 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index 01a1fde78..144423133 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -1,5 +1,6 @@ import re from typing import List +from urllib.parse import urlencode from django.utils.dateparse import parse_date @@ -55,15 +56,13 @@ def source_type(self): @staticmethod def get_ena_json_api(endpoint: str, accession: str, params: dict = {}): - query_params = "&".join([f"{k}={params[k]}" for k in params]) - if query_params: - query_params = f"&{query_params}" + params.update({"format": "json", "includeAccessions": accession}) - return f"{ENA_API}{endpoint}/?format=json&includeAccessions={accession}{query_params}" + return f"{ENA_API}{endpoint}/?{urlencode(params, )}" @staticmethod - def get_ena_json_api_response(endpoint: str, accession, params: dict = {}): + def get_ena_json_api_response(endpoint: str, accession: str, params: dict = {}): response = utils.requests_retry_session().get( SraSurveyor.get_ena_json_api(endpoint, accession, params) ) diff --git a/foreman/tests/surveyor/test_sra.py b/foreman/tests/surveyor/test_sra.py index b177553db..65e4eaf74 100644 --- a/foreman/tests/surveyor/test_sra.py +++ b/foreman/tests/surveyor/test_sra.py @@ -299,14 +299,15 @@ def test_sra_pubmed_metdata_is_applied(self): ) def test_sra_ena_api_endpoint_construction(self): - expected_filereport_url = "https://www.ebi.ac.uk/ena/portal/api/filereport/?format=json&accession=SRP047410&result=read_run" + expected_filereport_url = "https://www.ebi.ac.uk/ena/portal/api/filereport/?result=read_run&format=json&includeAccessions=SRP047410" + filereport_url = SraSurveyor.get_ena_json_api( "filereport", "SRP047410", {"result": "read_run"} ) self.assertEqual(expected_filereport_url, filereport_url) expected_metadata_url = ( - "https://www.ebi.ac.uk/ena/portal/api/search/?format=json&accession=SRP047410" + "https://www.ebi.ac.uk/ena/portal/api/search/?format=json&includeAccessions=SRP047410" ) metadata_url = SraSurveyor.get_ena_json_api("search", "SRP047410", {}) - self.assertEquual(expected_metadata_url, metadata_url) + self.assertEqual(expected_metadata_url, metadata_url) From 0d7ff958fed3e9bb6cd7f4a6f169e52c1828a12d Mon Sep 17 00:00:00 2001 From: David Mejia Date: Tue, 18 Jul 2023 13:37:34 -0400 Subject: [PATCH 179/220] remove ignore, setup.cfg instead --- codecov.yml | 4 ---- 1 file changed, 4 deletions(-) diff --git a/codecov.yml b/codecov.yml index 59eaf09fe..7d640b009 100644 --- a/codecov.yml +++ b/codecov.yml @@ -1,10 +1,6 @@ github_checks: annotations: false -ignore: - - "**/tests/**" - - "**/*.sh" - coverage: status: project: From bec7b89bb44a282bce6017883de8da3870187400 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 19 Jul 2023 09:26:21 -0700 Subject: [PATCH 180/220] Update smasher email sender address --- .../processors/smasher.py | 2 +- workers/tests/processors/test_smasher.py | 43 ++++++++++++++----- 2 files changed, 34 insertions(+), 11 deletions(-) diff --git a/workers/data_refinery_workers/processors/smasher.py b/workers/data_refinery_workers/processors/smasher.py index fa832925f..5a5c152a3 100644 --- a/workers/data_refinery_workers/processors/smasher.py +++ b/workers/data_refinery_workers/processors/smasher.py @@ -431,7 +431,7 @@ def _notify_send_email(job_context): """Send email notification to the user if the dataset succeded or failed.""" dataset_url = "https://www.refine.bio/dataset/" + str(job_context["dataset"].id) - SENDER = "Refine.bio Mail Robot " + SENDER = "refine.bio Datasets " RECIPIENT = job_context["dataset"].email_address SLACK_CCDL_EMAIL = "z7m4g8w4o6f5e0e7@alexslemonade.slack.com" CHARSET = "UTF-8" diff --git a/workers/tests/processors/test_smasher.py b/workers/tests/processors/test_smasher.py index 38e7760df..a1e8e2f72 100644 --- a/workers/tests/processors/test_smasher.py +++ b/workers/tests/processors/test_smasher.py @@ -1329,6 +1329,10 @@ def test_notify(self, mock_boto_client): self.assertTrue(final_context.get("success", True)) mock_boto_client.assert_called_once_with("ses", region_name="dummy region") ses_client_mock.send_email.assert_called_once() + self.assertEqual( + ses_client_mock.send_email.call_args.kwargs["Source"], + "refine.bio Datasets ", + ) # Now try explicitly setting notify_me false mock_boto_client.reset_mock() @@ -1383,7 +1387,10 @@ def setUp(self): "experiments": { "E-GEOD-44719": { "accession_code": "E-GEOD-44719", - "sample_accession_codes": ["IFNa DC_LB016_IFNa", "undefined_sample"], + "sample_accession_codes": [ + "IFNa DC_LB016_IFNa", + "undefined_sample", + ], } }, "samples": { @@ -1429,7 +1436,10 @@ def setUp(self): "name": "Sample_source_name", "value": "pineal glands at CT18, after light exposure", }, - {"name": "Sample_title", "value": "Pineal_Light_CT18"}, + { + "name": "Sample_title", + "value": "Pineal_Light_CT18", + }, ], }, # For single-key object whose key is "name", @@ -1466,7 +1476,8 @@ def setUp(self): # Create a sample attribute to check for contribution, _ = Contribution.objects.get_or_create( - source_name="MetaSRA", methods_url="https://pubmed.ncbi.nlm.nih.gov/28535296/" + source_name="MetaSRA", + methods_url="https://pubmed.ncbi.nlm.nih.gov/28535296/", ) s1 = Sample() @@ -1518,7 +1529,10 @@ def test_all_samples(self): "metadata": self.metadata, "dataset": Dataset.objects.create( aggregate_by="ALL", - data={"GSE56409": ["GSM1361050"], "E-GEOD-44719": ["E-GEOD-44719-GSM1089311"]}, + data={ + "GSE56409": ["GSM1361050"], + "E-GEOD-44719": ["E-GEOD-44719-GSM1089311"], + }, ), } smashing_utils.write_tsv_json(job_context) @@ -1562,7 +1576,10 @@ def test_experiment(self): "metadata": self.metadata, "dataset": Dataset.objects.create( aggregate_by="EXPERIMENT", - data={"GSE56409": ["GSM1361050"], "E-GEOD-44719": ["E-GEOD-44719-GSM1089311"]}, + data={ + "GSE56409": ["GSM1361050"], + "E-GEOD-44719": ["E-GEOD-44719-GSM1089311"], + }, ), } smashing_utils.write_tsv_json(job_context) @@ -1600,7 +1617,11 @@ def test_unicode_writer(self): "refinebio_accession_code": "eyy", "refinebio_annotations": [ # annotation #1 - {"😎😎": "😎😎", "detection_percentage": 98.44078, "mapped_percentage": 100.0} + { + "😎😎": "😎😎", + "detection_percentage": 98.44078, + "mapped_percentage": 100.0, + } ], # end of annotations }, # end of sample #1 }, # end of "samples" @@ -1675,7 +1696,10 @@ def test_species(self): "metadata": self.metadata, "dataset": Dataset.objects.create( aggregate_by="SPECIES", - data={"GSE56409": ["GSM1361050"], "E-GEOD-44719": ["E-GEOD-44719-GSM1089311"]}, + data={ + "GSE56409": ["GSM1361050"], + "E-GEOD-44719": ["E-GEOD-44719-GSM1089311"], + }, ), "group_by_keys": ["homo_sapiens", "fake_species"], "input_files": { @@ -1738,14 +1762,14 @@ def test_species(self): self.assertEqual(species_metadada["species"], "fake_species") self.assertEqual(len(species_metadada["samples"]), 1) self.assertEqual( - species_metadada["samples"][0]["refinebio_accession_code"], "E-GEOD-44719-GSM1089311" + species_metadada["samples"][0]["refinebio_accession_code"], + "E-GEOD-44719-GSM1089311", ) self.assertEqual(species_metadada["samples"][0]["MetaSRA_age"]["value"], 3.0) os.remove(json_filename) @tag("smasher") def test_bad_smash(self): - pj = ProcessorJob() pj.pipeline_applied = "SMASHER" pj.save() @@ -1852,7 +1876,6 @@ def test_bad_smash(self): @tag("smasher") def test_bad_overlap(self): - homo_sapiens = Organism(name="HOMO_SAPIENS", taxonomy_id=9606) homo_sapiens.save() From 2043f63a04901fedb2e139e6b093bcd27ac5d6fe Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 19 Jul 2023 11:05:24 -0700 Subject: [PATCH 181/220] Update docker credentials --- .github/scripts/update_docker_images.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/scripts/update_docker_images.sh b/.github/scripts/update_docker_images.sh index 06bdabbfd..881dbfceb 100755 --- a/.github/scripts/update_docker_images.sh +++ b/.github/scripts/update_docker_images.sh @@ -30,7 +30,7 @@ cd ~/refinebio/common && python3 setup.py sdist cd ~/refinebio # Log into DockerHub. -docker login -u "$DOCKER_ID" -p "$DOCKER_PASSWD" +docker login -u "$DOCKER_IO_USERNAME" -p "$DOCKER_IO_PASSWORD" IMAGE_NAMES="$WORKER_IMAGES foreman api" for IMAGE_NAME in $IMAGE_NAMES; do From 601a6f59343af4ee28c00eca0ed484984ab54804 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 19 Jul 2023 11:28:29 -0700 Subject: [PATCH 182/220] Update images --- .github/scripts/remote_deploy.sh | 2 +- .github/scripts/update_docker_images.sh | 10 ++++++---- .../{Dockerfile.api_production => Dockerfile.api} | 0 api/serve_production.sh | 4 ++-- scripts/common.sh | 4 +++- scripts/update_docker_images.sh | 4 ++-- 6 files changed, 14 insertions(+), 10 deletions(-) rename api/dockerfiles/{Dockerfile.api_production => Dockerfile.api} (100%) diff --git a/.github/scripts/remote_deploy.sh b/.github/scripts/remote_deploy.sh index f93c337c4..a82818a31 100755 --- a/.github/scripts/remote_deploy.sh +++ b/.github/scripts/remote_deploy.sh @@ -68,7 +68,7 @@ echo "Building new images" run_on_deploy_box "sudo touch /var/log/docker_update_$CI_TAG.log" run_on_deploy_box "sudo chown ubuntu:ubuntu /var/log/docker_update_$CI_TAG.log" run_on_deploy_box ". env_vars && echo -e '######\nBuilding new images for $CI_TAG\n######' 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" -run_on_deploy_box ". env_vars && .github/scripts/update_docker_images.sh 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" +run_on_deploy_box ". env_vars && ./.github/scripts/update_docker_images.sh 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" run_on_deploy_box ". env_vars && echo -e '######\nFinished building new images for $CI_TAG\n######' 2>&1 | tee -a /var/log/docker_update_$CI_TAG.log" # Load docker_image_exists function and $ALL_IMAGES. diff --git a/.github/scripts/update_docker_images.sh b/.github/scripts/update_docker_images.sh index 881dbfceb..093e40885 100755 --- a/.github/scripts/update_docker_images.sh +++ b/.github/scripts/update_docker_images.sh @@ -32,11 +32,13 @@ cd ~/refinebio # Log into DockerHub. docker login -u "$DOCKER_IO_USERNAME" -p "$DOCKER_IO_PASSWORD" -IMAGE_NAMES="$WORKER_IMAGES foreman api" -for IMAGE_NAME in $IMAGE_NAMES; do +for IMAGE_NAME in $ALL_IMAGES; do case $IMAGE_NAME in - api) - DOCKER_FILE_PATH="api/dockerfiles/Dockerfile.api_production" + api_base | api) + DOCKER_FILE_PATH="api/dockerfiles/Dockerfile.$IMAGE_NAME" + ;; + base) + DOCKER_FILE_PATH="common/dockerfiles/Dockerfile.$IMAGE_NAME" ;; foreman) DOCKER_FILE_PATH="foreman/dockerfiles/Dockerfile.$IMAGE_NAME" diff --git a/api/dockerfiles/Dockerfile.api_production b/api/dockerfiles/Dockerfile.api similarity index 100% rename from api/dockerfiles/Dockerfile.api_production rename to api/dockerfiles/Dockerfile.api diff --git a/api/serve_production.sh b/api/serve_production.sh index c23382778..5b04a69d0 100755 --- a/api/serve_production.sh +++ b/api/serve_production.sh @@ -14,7 +14,7 @@ cd "$script_directory" || exit # move up a level. cd .. -./scripts/prepare_image.sh -i api_production -s api +./scripts/prepare_image.sh -i api -s api . ./scripts/common.sh @@ -30,5 +30,5 @@ docker run \ --publish 8081:8081 \ --tty \ --volume "$STATIC_VOLUMES":/tmp/www/static \ - "$DOCKERHUB_REPO/dr_api_production" \ + "$DOCKERHUB_REPO/dr_api" \ /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" diff --git a/scripts/common.sh b/scripts/common.sh index acf22354d..f7d4cd705 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -2,10 +2,12 @@ # These are lists of docker images that we use. The actual names end # up being /dr_ but this is useful for scripting. -export ALL_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders foreman api" + # Sometimes we only want to work with the worker images. export WORKER_IMAGES="smasher compendia illumina affymetrix salmon transcriptome no_op downloaders" +export ALL_IMAGES="base api_base api foreman $WORKER_IMAGES" + # Default Docker registry. if [ -z "$DOCKERHUB_REPO" ]; then export DOCKERHUB_REPO="ccdlstaging" diff --git a/scripts/update_docker_images.sh b/scripts/update_docker_images.sh index 299974310..086301af9 100755 --- a/scripts/update_docker_images.sh +++ b/scripts/update_docker_images.sh @@ -78,7 +78,7 @@ DOCKER_ACTION="--push" # Intentionally omit affymetrix unless specifically requested since it is so # intense to build. -IMAGE_NAMES="base migrations common_tests foreman api_base api_production api_local \ +IMAGE_NAMES="base migrations common_tests foreman api_base api api_local \ transcriptome smasher salmon no_op illumina downloaders compendia" if [ "$BUILD_AFFYMETRIX" ]; then IMAGE_NAMES="$IMAGE_NAMES affymetrix" @@ -95,7 +95,7 @@ rm -f common/dist/* for IMAGE_NAME in $IMAGE_NAMES; do case $IMAGE_NAME in - api_base | api_local | api_production) + api_base | api | api_local) DOCKER_FILE_PATH="api/dockerfiles/Dockerfile.$IMAGE_NAME" ;; base | common_tests | migrations) From 3db25de589027b687209c263eb23e9d06a0a13e1 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 19 Jul 2023 12:13:09 -0700 Subject: [PATCH 183/220] Rename `get_master_or_dev` to `get_deploy_branch` everywhere --- .github/workflows/config.yml | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 13a43eef1..624562d09 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -570,7 +570,7 @@ jobs: - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables run: | source scripts/common.sh - echo "MASTER_OR_DEV=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV + echo "MASTER_OR_DEV=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV @@ -632,7 +632,7 @@ jobs: - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables run: | source scripts/common.sh - echo "MASTER_OR_DEV=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV + echo "MASTER_OR_DEV=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV @@ -679,4 +679,4 @@ jobs: name: Set the $CI_TAG Environment Variable run: | source scripts/common.sh - echo "branch=$(get_master_or_dev ${GITHUB_REF#refs/tags/})" >> $GITHUB_OUTPUT + echo "branch=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_OUTPUT From 5f62765cbc8b079165a434e961fd09683c89a7f5 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 19 Jul 2023 12:15:46 -0700 Subject: [PATCH 184/220] Use . instead of source --- .github/workflows/config.yml | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 624562d09..175e3a330 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -569,7 +569,7 @@ jobs: - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables run: | - source scripts/common.sh + . scripts/common.sh echo "MASTER_OR_DEV=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV @@ -631,7 +631,7 @@ jobs: - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables run: | - source scripts/common.sh + . scripts/common.sh echo "MASTER_OR_DEV=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV @@ -678,5 +678,5 @@ jobs: - id: set_branch name: Set the $CI_TAG Environment Variable run: | - source scripts/common.sh + . scripts/common.sh echo "branch=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_OUTPUT From 8ddba851f1a1e8cafd4073c934df9a3653ad4a2a Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Wed, 19 Jul 2023 19:32:39 -0700 Subject: [PATCH 185/220] Fix staging deploy --- .github/scripts/remote_deploy.sh | 8 ++++---- .github/workflows/config.yml | 8 ++++---- api/dockerfiles/Dockerfile.api | 3 ++- foreman/run_end_to_end_tests.sh | 5 +---- infrastructure/batch.tf | 2 +- infrastructure/instances.tf | 2 +- scripts/common.sh | 7 +------ 7 files changed, 14 insertions(+), 21 deletions(-) diff --git a/.github/scripts/remote_deploy.sh b/.github/scripts/remote_deploy.sh index a82818a31..ce6f00855 100755 --- a/.github/scripts/remote_deploy.sh +++ b/.github/scripts/remote_deploy.sh @@ -104,18 +104,18 @@ echo "Finished building new images, running run_terraform.sh." run_on_deploy_box "sudo touch /var/log/deploy_$CI_TAG.log" run_on_deploy_box "sudo chown ubuntu:ubuntu /var/log/deploy_$CI_TAG.log" run_on_deploy_box ". env_vars && echo -e '######\nStarting new deploy for $CI_TAG\n######' >> /var/log/deploy_$CI_TAG.log 2>&1" -run_on_deploy_box "sudo ./.github/scripts/update_ca_certificates.sh >> /var/log/deploy_$CI_TAG.log 2>&1" +run_on_deploy_box "sudo .github/scripts/update_ca_certificates.sh >> /var/log/deploy_$CI_TAG.log 2>&1" # This should never be logged to Github Actions in case it exposes any secrets as terraform output. -run_on_deploy_box ". env_vars && ./.github/scripts/run_terraform.sh >> /var/log/deploy_$CI_TAG.log 2>&1" +run_on_deploy_box ". env_vars && .github/scripts/run_terraform.sh >> /var/log/deploy_$CI_TAG.log 2>&1" run_on_deploy_box ". env_vars && echo -e '######\nDeploying $CI_TAG finished!\n######' >> /var/log/deploy_$CI_TAG.log 2>&1" -./.github/scripts/slackpost_deploy.sh robots deploybot +.github/scripts/slackpost_deploy.sh robots deploybot if [[ "$BRANCH" == "dev" ]]; then run_on_deploy_box ". env_vars && ./foreman/run_end_to_end_tests.sh" - ./.github/scripts/slackpost_end_to_end.sh robots deploybot + .github/scripts/slackpost_end_to_end.sh robots deploybot fi # Don't leave secrets lying around. diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 175e3a330..69b040603 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -567,10 +567,10 @@ jobs: with: fetch-depth: 0 - - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables + - name: Set the $BRANCH and $CI_TAG Environment Variables run: | . scripts/common.sh - echo "MASTER_OR_DEV=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV + echo "BRANCH=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV @@ -629,10 +629,10 @@ jobs: with: fetch-depth: 0 - - name: Set the $MASTER_OR_DEV and $CI_TAG Environment Variables + - name: Set the $BRANCH and $CI_TAG Environment Variables run: | . scripts/common.sh - echo "MASTER_OR_DEV=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV + echo "BRANCH=$(get_deploy_branch ${GITHUB_REF#refs/tags/})" >> $GITHUB_ENV # Remove /ref/tags/ from the beginning of the tag name echo "CI_TAG=${GITHUB_REF#refs/tags/}" >> $GITHUB_ENV diff --git a/api/dockerfiles/Dockerfile.api b/api/dockerfiles/Dockerfile.api index fccc67d0f..bbdf1af3e 100644 --- a/api/dockerfiles/Dockerfile.api +++ b/api/dockerfiles/Dockerfile.api @@ -5,7 +5,8 @@ FROM $DOCKERHUB_REPO/dr_api_base:$SYSTEM_VERSION # Fail in case of an error at any stage in the pipe. SHELL ["/bin/bash", "-o", "pipefail", "-c"] -RUN chmod +x /home/user/collect_and_run_uwsgi.sh && \ +RUN pip install --ignore-installed --no-cache-dir uwsgi && \ + chmod +x /home/user/collect_and_run_uwsgi.sh && \ mkdir -p /tmp/www/static && \ chown user /tmp/www/static diff --git a/foreman/run_end_to_end_tests.sh b/foreman/run_end_to_end_tests.sh index e08112ae2..c9879adc3 100755 --- a/foreman/run_end_to_end_tests.sh +++ b/foreman/run_end_to_end_tests.sh @@ -31,9 +31,6 @@ if [ ! -e "$reference_file_dir/$quant_file" ]; then "$test_data_repo/$quant_file" fi -# temp for testing locally. -../scripts/prepare_image.sh -i foreman -s foreman - while read -r row; do # Exporting an expansion rather than a variable, which is exactly what we want to do. # shellcheck disable=SC2163 @@ -50,5 +47,5 @@ docker run \ --volume "$HOME/.aws":/home/user/.aws \ --volume "$volume_directory":/home/user/data_store \ --tty \ - "$DOCKERHUB_REPO/dr_foreman" \ + "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ python3 manage.py test --no-input --parallel=2 --testrunner='tests.test_runner.NoDbTestRunner' tests.foreman.test_end_to_end diff --git a/infrastructure/batch.tf b/infrastructure/batch.tf index ae1ab8216..66bd1bb91 100644 --- a/infrastructure/batch.tf +++ b/infrastructure/batch.tf @@ -20,7 +20,7 @@ module "batch" { "workers-configuration/workers-instance-user-data.tpl.sh", { database_host = aws_instance.pg_bouncer.private_ip - database_name = aws_db_instance.postgres_db.name + database_name = aws_db_instance.postgres_db.db_name database_password = var.database_password database_port = var.database_port database_user = var.database_user diff --git a/infrastructure/instances.tf b/infrastructure/instances.tf index 0628c31d3..4fce9e5ac 100644 --- a/infrastructure/instances.tf +++ b/infrastructure/instances.tf @@ -195,7 +195,7 @@ resource "aws_instance" "foreman_server_1" { { accession_gathering_job_run_day = var.accession_gathering_job_run_day database_host = aws_instance.pg_bouncer.private_ip - database_name = aws_db_instance.postgres_db.name + database_name = aws_db_instance.postgres_db.db_name database_password = var.database_password database_user = var.database_user dockerhub_repo = var.dockerhub_repo diff --git a/scripts/common.sh b/scripts/common.sh index 29609f6ad..8f9b5e59a 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -133,12 +133,6 @@ update_docker_image() { CACHE_TO_LATEST="type=registry,ref=${DOCKERHUB_IMAGE}_cache:latest,mode=max" CACHE_TO_VERSION="type=registry,ref=${DOCKERHUB_IMAGE}_cache:$SYSTEM_VERSION,mode=max" - if test "$GITHUB_ACTION"; then - CACHE_TO_LATEST="type=gha" - CACHE_TO_VERSION="type=gha" - DOCKER_ACTION="--push" - fi - set_up_docker_builder docker buildx build \ @@ -155,6 +149,7 @@ update_docker_image() { "$DOCKER_ACTION" \ . } + # Default Docker registry. if [ -z "$DOCKERHUB_REPO" ]; then export DOCKERHUB_REPO="ccdlstaging" From 30276efe73eeffbfb68a0b1a136db2015f78bd26 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 20 Jul 2023 07:39:40 -0700 Subject: [PATCH 186/220] Fix common/foreman tests --- .github/workflows/config.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 69b040603..be68e8709 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -207,7 +207,7 @@ jobs: env: IMAGES: migrations common_tests needs: - - test_base + - test_api runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 @@ -291,7 +291,7 @@ jobs: env: IMAGES: migrations foreman needs: - - test_base + - test_api runs-on: ubuntu-latest steps: - uses: actions/checkout@v3 From 7a71dfbfae022b0248abc7bd06300319e0135c51 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 20 Jul 2023 10:35:24 -0700 Subject: [PATCH 187/220] Move downloaders and no_op tests to larger runners --- .github/workflows/config.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index be68e8709..41e956897 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -255,7 +255,7 @@ jobs: IMAGES: migrations api_base api_local downloaders foreman needs: - test_base - runs-on: ubuntu-latest + runs-on: ubuntu-latest-m steps: - uses: actions/checkout@v3 From a7c99df94fe7e559ac191839b2491efa1b4bbb1a Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 24 Jul 2023 15:14:21 -0400 Subject: [PATCH 188/220] prevent age from being set as string, adds test --- .../data_refinery_foreman/surveyor/harmony.py | 5 +- foreman/tests/surveyor/test_geo.py | 14 + .../surveyor.geo.geo_survey_GSE94793.yaml | 38965 ++++++++++++++++ 3 files changed, 38982 insertions(+), 2 deletions(-) create mode 100644 test_volume/cassettes/surveyor.geo.geo_survey_GSE94793.yaml diff --git a/foreman/data_refinery_foreman/surveyor/harmony.py b/foreman/data_refinery_foreman/surveyor/harmony.py index 2706adb89..b0fb7996d 100644 --- a/foreman/data_refinery_foreman/surveyor/harmony.py +++ b/foreman/data_refinery_foreman/surveyor/harmony.py @@ -784,8 +784,9 @@ def harmonize_field( harmonized_values.add(harmonized_value) # Age can only be one value in the database. - if sample_attribute == "age" and len(harmonized_values) > 0: - harmonized_sample[sample_attribute] = harmonized_values.pop() + if sample_attribute == "age": + if len(harmonized_values) > 0: + harmonized_sample[sample_attribute] = harmonized_values.pop() else: harmonized_sample[sample_attribute] = ";".join( sorted([str(v) for v in harmonized_values]) diff --git a/foreman/tests/surveyor/test_geo.py b/foreman/tests/surveyor/test_geo.py index cd93b282f..85793c685 100644 --- a/foreman/tests/surveyor/test_geo.py +++ b/foreman/tests/surveyor/test_geo.py @@ -84,6 +84,20 @@ def test_geo_survey_GSE6532(self, mock_send_task): self.assertEqual(741, Sample.objects.all().count()) + @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.geo.geo_survey_GSE94793.yaml") + @patch("data_refinery_foreman.surveyor.external_source.send_job") + def test_geo_survey_GSE94793(self, mock_send_task): + """This experiment revealed an issue with age being saved as a string. + + Seemed like a good test to make permanent. + """ + self.prep_test("GSE94793") + + geo_surveyor = GeoSurveyor(self.survey_job) + geo_surveyor.survey() + + self.assertEqual(24, Sample.objects.all().count()) + @vcr.use_cassette("/home/user/data_store/cassettes/surveyor.geo.geo_survey_not_agilent.yaml") @patch("data_refinery_foreman.surveyor.external_source.send_job") def test_geo_survey_not_agilent(self, mock_send_task): diff --git a/test_volume/cassettes/surveyor.geo.geo_survey_GSE94793.yaml b/test_volume/cassettes/surveyor.geo.geo_survey_GSE94793.yaml new file mode 100644 index 000000000..0cca817d9 --- /dev/null +++ b/test_volume/cassettes/surveyor.geo.geo_survey_GSE94793.yaml @@ -0,0 +1,38965 @@ +interactions: +- request: + body: null + headers: + Accept: + - 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status: + code: 200 + message: OK +version: 1 From bd5ee0eb02a2adca1b27c0caa1eafb44bd869353 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 25 Jul 2023 07:53:24 -0700 Subject: [PATCH 189/220] Correct SRA surveyor method typo --- foreman/data_refinery_foreman/surveyor/sra.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index 144423133..6a53f6107 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -281,7 +281,7 @@ def _generate_experiment_and_samples( experiment_or_sample_accession ) - experiment = self._generate_experment(experiment_metadata) + experiment = self._generate_experiment(experiment_metadata) samples = [ self._generate_sample(experiment, sample_metadata) for sample_metadata in samples_metadata From 65d01d32afdb428f301dfe2902ee4191067a5685 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 25 Jul 2023 17:14:45 -0700 Subject: [PATCH 190/220] Update experiment generation method to obtain experiment object correctly --- foreman/data_refinery_foreman/surveyor/sra.py | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index 6a53f6107..a258a803d 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -299,7 +299,9 @@ def _generate_experiment(self, experiment_metadata: dict) -> Experiment: # study_accession is PRJDB etc experiment_accession = experiment_metadata.get("secondary_study_accession") - experiment, is_created = Experiment.get_or_create(accession_code=experiment_accession) + experiment, is_created = Experiment.objects.get_or_create( + accession_code=experiment_accession + ) if is_created: SraSurveyor._apply_metadata_to_experiment(experiment, experiment_metadata) From 71dd9f2c3ed7fb279d61658b45dabe94d4c573e4 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 31 Jul 2023 11:49:34 -0400 Subject: [PATCH 191/220] sra file report params --- foreman/data_refinery_foreman/surveyor/sra.py | 7 ++++++- 1 file changed, 6 insertions(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index a258a803d..007f737f0 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -57,7 +57,10 @@ def source_type(self): @staticmethod def get_ena_json_api(endpoint: str, accession: str, params: dict = {}): - params.update({"format": "json", "includeAccessions": accession}) + params.update({"format": "json"}) + + if "accession" not in params: + params.update({"includeAccessions": accession}) return f"{ENA_API}{endpoint}/?{urlencode(params, )}" @@ -114,6 +117,8 @@ def gather_file_report(run_accession: str): "filereport", run_accession, { + "accession": run_accession, + "result": "read_run", "fields": "all", }, ) From 013703e04c1c12943c82996fcdeeb099538eaa44 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Tue, 1 Aug 2023 11:59:55 -0400 Subject: [PATCH 192/220] refactor sample_protocols update --- foreman/data_refinery_foreman/surveyor/sra.py | 34 +++++++++---------- 1 file changed, 16 insertions(+), 18 deletions(-) diff --git a/foreman/data_refinery_foreman/surveyor/sra.py b/foreman/data_refinery_foreman/surveyor/sra.py index 007f737f0..aa7d00835 100644 --- a/foreman/data_refinery_foreman/surveyor/sra.py +++ b/foreman/data_refinery_foreman/surveyor/sra.py @@ -217,7 +217,9 @@ def _apply_metadata_to_experiment(experiment: Experiment, metadata: dict): experiment.source_first_published = parse_date(metadata["first_public"]) experiment.source_last_modified = parse_date(metadata["last_updated"]) experiment.alternate_accession_code = utils.get_nonempty(metadata, "geo_accession", None) - experiment.protocol_description = utils.get_nonempty(metadata, "protocol", []) + experiment.protocol_description = utils.get_nonempty( + metadata, "protocol", "Protocol was never provided." + ) experiment.pubmed_id = metadata.get("pubmed_id", "") experiment.has_publication = "pubmed_id" in metadata @@ -375,13 +377,7 @@ def _generate_sample(self, experiment: Experiment, sample_metadata: dict) -> Sam survey_job=self.survey_job.id, ) - # i think we should be collecting the sample protocols - # on the experiment here instead - protocol_info, protocol_updated = self.update_sample_protocol_info( - existing_protocols=sample.protocol_info, - experiment_protocol=experiment.protocol_description, - experiment_url=experiment.source_url, - ) + protocol_info, protocol_updated = self.update_sample_protocol_info(sample, experiment) if sample_created or protocol_updated: sample.protocol_info = protocol_info @@ -397,7 +393,7 @@ def _generate_sample(self, experiment: Experiment, sample_metadata: dict) -> Sam return sample @staticmethod - def update_sample_protocol_info(existing_protocols, experiment_protocol, experiment_url): + def update_sample_protocol_info(sample: Sample, experiment: Experiment): """Compares experiment_protocol with a sample's existing_protocols and update the latter if the former is new. @@ -407,21 +403,23 @@ def update_sample_protocol_info(existing_protocols, experiment_protocol, experim """ # ensure we are working with a list # since the default value for a JSON field is an empty dict - existing_protocols_list = [] - if existing_protocols is not {}: - existing_protocols_list = existing_protocols + sample_protocols = sample.protocol_info if isinstance(sample.protocol_info, list) else [] + # Exit if no protocol provided. + experiment_protocol = experiment.protocol_description if experiment_protocol == "Protocol was never provided.": - return (existing_protocols_list, False) + return sample_protocols, False - existing_descriptions = [protocol["Description"] for protocol in existing_protocols_list] + # Exit if protocol is already accounted for. + existing_descriptions = [protocol["Description"] for protocol in sample_protocols] if experiment_protocol in existing_descriptions: - return (existing_protocols_list, False) + return sample_protocols, False - existing_protocols_list.append( - {"Description": experiment_protocol, "Reference": experiment_url} + sample_protocols.append( + {"Description": experiment.protocol_description, "Reference": experiment.source_url} ) - return (existing_protocols_list, True) + + return sample_protocols, True def discover_experiment_and_samples(self): """Returns an experiment and a list of samples for an SRA accession""" From 4261bb9fdb416894934c2d72cd2ca1ff360fea0b Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 2 Aug 2023 16:38:44 -0400 Subject: [PATCH 193/220] update test for working sample --- workers/tests/downloaders/test_sra.py | 11 ++++++----- 1 file changed, 6 insertions(+), 5 deletions(-) diff --git a/workers/tests/downloaders/test_sra.py b/workers/tests/downloaders/test_sra.py index 674c2e04d..53e40c2d3 100644 --- a/workers/tests/downloaders/test_sra.py +++ b/workers/tests/downloaders/test_sra.py @@ -26,17 +26,17 @@ def insert_objects(self): @tag("downloaders_sra") def test_download_file(self): dlj = DownloaderJob() - dlj.accession_code = "ERR036" + dlj.accession_code = "SRR24086949" dlj.save() og = OriginalFile() - og.source_filename = "ERR036000.fastq.gz" - og.source_url = "ftp.sra.ebi.ac.uk/vol1/fastq/ERR036/ERR036000/ERR036000_1.fastq.gz" + og.source_filename = "SRR24086949.fastq.gz" + og.source_url = "ftp.sra.ebi.ac.uk/vol1/fastq/SRR240/049/SRR24086949/SRR24086949_1.fastq.gz" og.is_archive = True og.save() sample = Sample() - sample.accession_code = "ERR036000" + sample.accession_code = "SRR24086949" sample.save() assoc = OriginalFileSampleAssociation() @@ -52,7 +52,8 @@ def test_download_file(self): result, downloaded_files = sra.download_sra(dlj.pk) self.assertTrue(result) - self.assertEqual(downloaded_files[0].sha1, "1dfe5460a4101fe87feeffec0cb2e053f6695961") + self.assertEqual(downloaded_files[0].sha1, "ef52900be820e4001988e64b7d664f7af595e581") + print(downloaded_files[0].sha1) self.assertTrue(os.path.exists(downloaded_files[0].absolute_file_path)) @tag("downloaders") From 2b293e8ddc245182885d501ea0d75b045c5355cb Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 2 Aug 2023 16:40:21 -0400 Subject: [PATCH 194/220] add ENA info logs --- workers/data_refinery_workers/downloaders/sra.py | 2 ++ 1 file changed, 2 insertions(+) diff --git a/workers/data_refinery_workers/downloaders/sra.py b/workers/data_refinery_workers/downloaders/sra.py index 9008f0d4d..4003919e7 100644 --- a/workers/data_refinery_workers/downloaders/sra.py +++ b/workers/data_refinery_workers/downloaders/sra.py @@ -100,11 +100,13 @@ def _download_file_aspera( # and we are not using any kind of rate limiting. command_str = "ascp -QT -l 300m -P 33001 -i keys/asperaweb_id_dsa.openssh {src} {dest}" formatted_command = command_str.format(src=download_url, dest=target_file_path) + logger.info("Starting ENA ascp", time=str(timezone.now())) completed_command = subprocess.run( formatted_command.split(), stdout=subprocess.PIPE, stderr=subprocess.PIPE, ) + logger.info("Ending ENA ascp", time=str(timezone.now())) else: # NCBI requires encryption and recommends -k1 resume, as # well as the 450m limit and -Q (play fair). From 17cde61df0243271133989dab63fefc53c58de74 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Wed, 2 Aug 2023 16:41:29 -0400 Subject: [PATCH 195/220] add original_file parameter on retry --- workers/data_refinery_workers/downloaders/sra.py | 9 +++++++-- 1 file changed, 7 insertions(+), 2 deletions(-) diff --git a/workers/data_refinery_workers/downloaders/sra.py b/workers/data_refinery_workers/downloaders/sra.py index 4003919e7..c759f7542 100644 --- a/workers/data_refinery_workers/downloaders/sra.py +++ b/workers/data_refinery_workers/downloaders/sra.py @@ -147,7 +147,12 @@ def _download_file_aspera( else: time.sleep(5) return _download_file_aspera( - download_url, downloader_job, target_file_path, attempt + 1, source + download_url, + downloader_job, + target_file_path, + attempt + 1, + original_file, + source, ) except Exception: logger.exception( @@ -176,7 +181,7 @@ def _download_file_aspera( ) time.sleep(10) return _download_file_aspera( - download_url, downloader_job, target_file_path, attempt + 1, source + download_url, downloader_job, target_file_path, attempt + 1, original_file, source ) return True From ee62a16f021c5464c88a4cb549e922e40a3deadb Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 3 Aug 2023 09:59:54 -0700 Subject: [PATCH 196/220] Fix API server user data error --- .../api-configuration/api-server-instance-user-data.tpl.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh b/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh index 0dfb15ddc..0a830bc2b 100644 --- a/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh +++ b/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh @@ -151,7 +151,7 @@ docker run \ --name=dr_api \ --tty \ --volume "$STATIC_VOLUMES":/tmp/www/static \ - -publish 8081:8081 \ + --publish 8081:8081 \ "${dockerhub_repo}/${api_docker_image}" \ /bin/sh -c "/home/user/collect_and_run_uwsgi.sh" From 179b15877f5671de5264094e1102be25563753e8 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Thu, 3 Aug 2023 13:25:21 -0400 Subject: [PATCH 197/220] adjust asserted counts for missing AE samples --- foreman/tests/foreman/test_end_to_end.py | 20 +++++++++++++++++--- 1 file changed, 17 insertions(+), 3 deletions(-) diff --git a/foreman/tests/foreman/test_end_to_end.py b/foreman/tests/foreman/test_end_to_end.py index 918db83d7..76ba09b64 100644 --- a/foreman/tests/foreman/test_end_to_end.py +++ b/foreman/tests/foreman/test_end_to_end.py @@ -54,6 +54,11 @@ "SRP094706", # 4 samples of SACCHAROMYCES_CEREVISIAE RNA-Seq data ] +# TODO: remove after array express samples are enabled. +ARRAY_EXPRESS_SAMPLES_COUNT = 39 + +EXPECTED_TOTAL_SAMPLES_COUNT = 191 - ARRAY_EXPRESS_SAMPLES_COUNT + EXPERIMENT_ACCESSION_CODES = MICROARRAY_ACCESSION_CODES + RNA_SEQ_ACCESSION_CODES TEST_DATA_BUCKET = "data-refinery-test-assets" @@ -285,7 +290,9 @@ def process_experiments(self): experiment__accession_code__in=EXPERIMENT_ACCESSION_CODES ).values_list("id") - self.assertEqual(Sample.objects.filter(id__in=sample_id_list).count(), 191) + self.assertEqual( + Sample.objects.filter(id__in=sample_id_list).count(), EXPECTED_TOTAL_SAMPLES_COUNT + ) samples = [] for accession_code in EXPERIMENT_ACCESSION_CODES: @@ -329,7 +336,11 @@ def process_experiments(self): self.assertTrue(wait_for_job(processor_job)) # Because SRR1583739 fails, the 26 samples from SRP047410 won't be processed - self.assertEqual(Sample.processed_objects.filter(id__in=sample_id_list).count(), 165) + EXPECTED_SAMPLE_FAILURES = 26 + self.assertEqual( + Sample.processed_objects.filter(id__in=sample_id_list).count(), + EXPECTED_TOTAL_SAMPLES_COUNT - EXPECTED_SAMPLE_FAILURES, + ) print("Finally, need to run tximport to finish an experiment with one bad sample.") tximport_jobs = run_tximport_for_all_eligible_experiments(dispatch_jobs=False) @@ -338,7 +349,10 @@ def process_experiments(self): self.assertTrue(wait_for_job(tximport_jobs[0])) # This is the full total of jobs minus one. - self.assertEqual(Sample.processed_objects.filter(id__in=sample_id_list).count(), 190) + self.assertEqual( + Sample.processed_objects.filter(id__in=sample_id_list).count(), + EXPECTED_TOTAL_SAMPLES_COUNT - 1, + ) def check_transcriptome_index(self): # Make sure that a processed file using our new transcriptome index is From 8e80458f85814189a8c3f8c09961741d374a1238 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 7 Aug 2023 16:21:58 -0400 Subject: [PATCH 198/220] adding debug lines --- foreman/tests/foreman/test_end_to_end.py | 3 +++ 1 file changed, 3 insertions(+) diff --git a/foreman/tests/foreman/test_end_to_end.py b/foreman/tests/foreman/test_end_to_end.py index 76ba09b64..f6104bcfe 100644 --- a/foreman/tests/foreman/test_end_to_end.py +++ b/foreman/tests/foreman/test_end_to_end.py @@ -59,6 +59,9 @@ EXPECTED_TOTAL_SAMPLES_COUNT = 191 - ARRAY_EXPRESS_SAMPLES_COUNT +print("Array Express samples count:", ARRAY_EXPRESS_SAMPLES_COUNT) +print("Expected total samples count:", EXPECTED_TOTAL_SAMPLES_COUNT) + EXPERIMENT_ACCESSION_CODES = MICROARRAY_ACCESSION_CODES + RNA_SEQ_ACCESSION_CODES TEST_DATA_BUCKET = "data-refinery-test-assets" From fe66ff1b9cfa1f96c43053bc353b195d69058dc5 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 10 Aug 2023 10:16:39 -0700 Subject: [PATCH 199/220] Update deploy configuration - Explicitly set docker commands platform option - Update S3 buckets ACL/lifecycle configuration syntax --- foreman/run_end_to_end_tests.sh | 1 + .../api-server-instance-user-data.tpl.sh | 4 +- infrastructure/deploy.sh | 5 +- infrastructure/disk.tf | 256 +++++++++++++++--- 4 files changed, 220 insertions(+), 46 deletions(-) diff --git a/foreman/run_end_to_end_tests.sh b/foreman/run_end_to_end_tests.sh index c9879adc3..36a16e969 100755 --- a/foreman/run_end_to_end_tests.sh +++ b/foreman/run_end_to_end_tests.sh @@ -44,6 +44,7 @@ docker run \ --env REFINEBIO_BASE_URL="http://$API_HOST/v1/" \ --env RUNNING_IN_CLOUD=False \ --env-file ../infrastructure/prod_env \ + --platform linux/amd64 \ --volume "$HOME/.aws":/home/user/.aws \ --volume "$volume_directory":/home/user/data_store \ --tty \ diff --git a/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh b/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh index 0a830bc2b..8ed9ba8d8 100644 --- a/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh +++ b/infrastructure/api-configuration/api-server-instance-user-data.tpl.sh @@ -144,11 +144,11 @@ docker run \ --env ELASTICSEARCH_PORT="${elasticsearch_port}" \ --env-file environment \ --interactive \ - --log-driver=awslogs \ + --log-driver awslogs \ --log-opt awslogs-group="${log_group}" \ --log-opt awslogs-region="${region}" \ --log-opt awslogs-stream="${log_stream}" \ - --name=dr_api \ + --name dr_api \ --tty \ --volume "$STATIC_VOLUMES":/tmp/www/static \ --publish 8081:8081 \ diff --git a/infrastructure/deploy.sh b/infrastructure/deploy.sh index 2298c2d8a..2a49e0f14 100755 --- a/infrastructure/deploy.sh +++ b/infrastructure/deploy.sh @@ -267,7 +267,7 @@ done echo "Job registrations have been fired off." # Get an image to run the migrations with. -docker pull "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" +docker pull --platform linux/amd64 "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" # Test that the pg_bouncer instance is up. 15 minutes should be more than enough. start_time=$(date +%s) @@ -288,6 +288,7 @@ docker run \ --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ --env RUNNING_IN_CLOUD=False \ --env-file prod_env \ + --platform linux/amd64 \ "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ python3 manage.py migrate auth @@ -296,6 +297,7 @@ docker run \ --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ --env RUNNING_IN_CLOUD=False \ --env-file prod_env \ + --platform linux/amd64 \ "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ python3 manage.py migrate @@ -304,6 +306,7 @@ docker run \ --env DATABASE_HOST="$DATABASE_PUBLIC_HOST" \ --env RUNNING_IN_CLOUD=False \ --env-file prod_env \ + --platform linux/amd64 \ "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" \ python3 manage.py createcachetable diff --git a/infrastructure/disk.tf b/infrastructure/disk.tf index e7b357132..0754378fa 100644 --- a/infrastructure/disk.tf +++ b/infrastructure/disk.tf @@ -1,126 +1,268 @@ resource "aws_s3_bucket" "data_refinery_bucket" { - bucket = "data-refinery-s3-${var.user}-${var.stage}" - acl = "private" + bucket = "data-refinery-s3-${var.user}-${var.stage}" force_destroy = var.static_bucket_prefix == "dev" ? true : false tags = merge( var.default_tags, { - Name = "data-refinery-s3-${var.user}-${var.stage}" + Name = "data-refinery-s3-${var.user}-${var.stage}" Environment = var.stage } ) } +resource "aws_s3_bucket_ownership_controls" "data_refinery_bucket" { + bucket = aws_s3_bucket.data_refinery_bucket.id + rule { + object_ownership = "BucketOwnerPreferred" + } +} + resource "aws_s3_bucket_public_access_block" "data_refinery_bucket" { bucket = aws_s3_bucket.data_refinery_bucket.id block_public_acls = true block_public_policy = true + ignore_public_acls = true +} + +resource "aws_s3_bucket_acl" "data_refinery_bucket" { + depends_on = [ + aws_s3_bucket_ownership_controls.data_refinery_bucket, + aws_s3_bucket_public_access_block.data_refinery_bucket, + ] + + bucket = aws_s3_bucket.data_refinery_bucket.id + acl = "private" } resource "aws_s3_bucket" "data_refinery_results_bucket" { - bucket = "data-refinery-s3-results-${var.user}-${var.stage}" - acl = "private" + bucket = "data-refinery-s3-results-${var.user}-${var.stage}" force_destroy = var.static_bucket_prefix == "dev" ? true : false tags = merge( var.default_tags, { - Name = "data-refinery-s3-results-${var.user}-${var.stage}" + Name = "data-refinery-s3-results-${var.user}-${var.stage}" Environment = var.stage } ) - lifecycle_rule { - id = "auto-delete-after-7-days-${var.user}-${var.stage}" - prefix = "" - enabled = true - abort_incomplete_multipart_upload_days = 1 +} + +resource "aws_s3_bucket_ownership_controls" "data_refinery_results_bucket" { + bucket = aws_s3_bucket.data_refinery_results_bucket.id + rule { + object_ownership = "BucketOwnerPreferred" + } +} + +resource "aws_s3_bucket_public_access_block" "data_refinery_results_bucket" { + bucket = aws_s3_bucket.data_refinery_results_bucket.id + + block_public_acls = true + block_public_policy = true + ignore_public_acls = true +} + +resource "aws_s3_bucket_acl" "data_refinery_results_bucket" { + depends_on = [ + aws_s3_bucket_ownership_controls.data_refinery_results_bucket, + aws_s3_bucket_public_access_block.data_refinery_results_bucket, + ] + + bucket = aws_s3_bucket.data_refinery_results_bucket.id + acl = "private" +} + +resource "aws_s3_bucket_lifecycle_configuration" "data_refinery_results_bucket" { + bucket = aws_s3_bucket.data_refinery_results_bucket.id + + rule { + id = "auto-delete-after-7-days-${var.user}-${var.stage}" + status = "Enabled" + + abort_incomplete_multipart_upload { + days_after_initiation = 1 + } expiration { - days = 7 + days = 7 expired_object_delete_marker = true } noncurrent_version_expiration { - days = 1 + noncurrent_days = 1 } } } + resource "aws_s3_bucket" "data_refinery_transcriptome_index_bucket" { - bucket = "data-refinery-s3-transcriptome-index-${var.user}-${var.stage}" - acl = "public-read" + bucket = "data-refinery-s3-transcriptome-index-${var.user}-${var.stage}" force_destroy = var.static_bucket_prefix == "dev" ? true : false tags = merge( var.default_tags, { - Name = "data-refinery-s3-transcriptome-index-${var.user}-${var.stage}" + Name = "data-refinery-s3-transcriptome-index-${var.user}-${var.stage}" Environment = var.stage } ) } +resource "aws_s3_bucket_ownership_controls" "data_refinery_transcriptome_index_bucket" { + bucket = aws_s3_bucket.data_refinery_transcriptome_index_bucket.id + rule { + object_ownership = "BucketOwnerPreferred" + } +} + +resource "aws_s3_bucket_public_access_block" "data_refinery_transcriptome_index_bucket" { + bucket = aws_s3_bucket.data_refinery_transcriptome_index_bucket.id + + block_public_acls = false + block_public_policy = false + ignore_public_acls = false + restrict_public_buckets = false +} + +resource "aws_s3_bucket_acl" "data_refinery_transcriptome_index_bucket" { + depends_on = [ + aws_s3_bucket_ownership_controls.data_refinery_transcriptome_index_bucket, + aws_s3_bucket_public_access_block.data_refinery_transcriptome_index_bucket, + ] + + bucket = aws_s3_bucket.data_refinery_transcriptome_index_bucket.id + acl = "public-read" +} + resource "aws_s3_bucket" "data_refinery_qn_target_bucket" { - bucket = "data-refinery-s3-qn-target-${var.user}-${var.stage}" - acl = "public-read" + bucket = "data-refinery-s3-qn-target-${var.user}-${var.stage}" force_destroy = var.static_bucket_prefix == "dev" ? true : false tags = merge( var.default_tags, { - Name = "data-refinery-s3-qn-target-${var.user}-${var.stage}" + Name = "data-refinery-s3-qn-target-${var.user}-${var.stage}" Environment = var.stage } ) } +resource "aws_s3_bucket_ownership_controls" "data_refinery_qn_target_bucket" { + bucket = aws_s3_bucket.data_refinery_qn_target_bucket.id + rule { + object_ownership = "BucketOwnerPreferred" + } +} + +resource "aws_s3_bucket_public_access_block" "data_refinery_qn_target_bucket" { + bucket = aws_s3_bucket.data_refinery_qn_target_bucket.id + + block_public_acls = false + block_public_policy = false + ignore_public_acls = false + restrict_public_buckets = false +} + +resource "aws_s3_bucket_acl" "data_refinery_qn_target_bucket" { + depends_on = [ + aws_s3_bucket_ownership_controls.data_refinery_qn_target_bucket, + aws_s3_bucket_public_access_block.data_refinery_qn_target_bucket, + ] + + bucket = aws_s3_bucket.data_refinery_qn_target_bucket.id + acl = "public-read" +} + resource "aws_s3_bucket" "data_refinery_compendia_bucket" { - bucket = "data-refinery-s3-compendia-${var.user}-${var.stage}" - acl = "private" + bucket = "data-refinery-s3-compendia-${var.user}-${var.stage}" force_destroy = var.static_bucket_prefix == "dev" ? true : false tags = merge( var.default_tags, { - Name = "data-refinery-s3-compendia-${var.user}-${var.stage}" + Name = "data-refinery-s3-compendia-${var.user}-${var.stage}" Environment = var.stage } ) } +resource "aws_s3_bucket_ownership_controls" "data_refinery_compendia_bucket" { + bucket = aws_s3_bucket.data_refinery_compendia_bucket.id + rule { + object_ownership = "BucketOwnerPreferred" + } +} + resource "aws_s3_bucket_public_access_block" "data_refinery_compendia_bucket" { bucket = aws_s3_bucket.data_refinery_compendia_bucket.id block_public_acls = true block_public_policy = true + ignore_public_acls = true +} + +resource "aws_s3_bucket_acl" "data_refinery_compendia_bucket" { + depends_on = [ + aws_s3_bucket_ownership_controls.data_refinery_compendia_bucket, + aws_s3_bucket_public_access_block.data_refinery_compendia_bucket, + ] + + bucket = aws_s3_bucket.data_refinery_compendia_bucket.id + acl = "private" } + resource "aws_s3_bucket" "data_refinery_cloudtrail_logs_bucket" { - bucket = "data-refinery-s3-cloudtrail-logs-${var.user}-${var.stage}" - acl = "private" + bucket = "data-refinery-s3-cloudtrail-logs-${var.user}-${var.stage}" force_destroy = var.static_bucket_prefix == "dev" ? true : false tags = merge( var.default_tags, { - Name = "data-refinery-s3-cloudtrail-logs-${var.user}-${var.stage}" + Name = "data-refinery-s3-cloudtrail-logs-${var.user}-${var.stage}" Environment = var.stage } ) } +resource "aws_s3_bucket_ownership_controls" "data_refinery_cloudtrail_logs_bucket" { + bucket = aws_s3_bucket.data_refinery_cloudtrail_logs_bucket.id + rule { + object_ownership = "BucketOwnerPreferred" + } +} + +resource "aws_s3_bucket_public_access_block" "data_refinery_cloudtrail_logs_bucket" { + bucket = aws_s3_bucket.data_refinery_cloudtrail_logs_bucket.id + + block_public_acls = true + block_public_policy = true + ignore_public_acls = true +} + +resource "aws_s3_bucket_acl" "data_refinery_cloudtrail_logs_bucket" { + depends_on = [ + aws_s3_bucket_ownership_controls.data_refinery_cloudtrail_logs_bucket, + aws_s3_bucket_public_access_block.data_refinery_cloudtrail_logs_bucket, + ] + + bucket = aws_s3_bucket.data_refinery_cloudtrail_logs_bucket.id + acl = "private" +} + + # Passing the name attribute as `EntireBucket` enables request metrics for the bucket. # ref: https://www.terraform.io/docs/providers/aws/r/s3_bucket_metric.html resource "aws_s3_bucket_metric" "compendia_bucket_metrics" { bucket = aws_s3_bucket.data_refinery_compendia_bucket.bucket - name = "EntireBucket" + name = "EntireBucket" } resource "aws_cloudwatch_event_rule" "compendia_object_metrics" { - name = "data-refinery-compendia-object-metric-${var.user}-${var.stage}" + name = "data-refinery-compendia-object-metric-${var.user}-${var.stage}" description = "Download Compendia Events" event_pattern = < Date: Wed, 16 Aug 2023 14:07:38 -0700 Subject: [PATCH 200/220] Use unique names for docker renv loading commands --- common/dockerfiles/Dockerfile.common_tests | 4 ++-- workers/dockerfiles/Dockerfile.affymetrix | 4 ++-- workers/dockerfiles/Dockerfile.compendia | 4 ++-- workers/dockerfiles/Dockerfile.downloaders | 12 +++++++----- workers/dockerfiles/Dockerfile.illumina | 4 ++-- workers/dockerfiles/Dockerfile.no_op | 4 ++-- workers/dockerfiles/Dockerfile.salmon | 4 ++-- workers/dockerfiles/Dockerfile.smasher | 4 ++-- 8 files changed, 21 insertions(+), 19 deletions(-) diff --git a/common/dockerfiles/Dockerfile.common_tests b/common/dockerfiles/Dockerfile.common_tests index d914a52f0..3d5cf4f05 100644 --- a/common/dockerfiles/Dockerfile.common_tests +++ b/common/dockerfiles/Dockerfile.common_tests @@ -8,8 +8,8 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY common/R/dependencies/common_tests/renv.lock . -COPY common/R/renv_load.R . -RUN Rscript renv_load.R +COPY common/R/renv_load.R renv_load_common_tests.R +RUN Rscript renv_load_common_tests.R COPY common/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt diff --git a/workers/dockerfiles/Dockerfile.affymetrix b/workers/dockerfiles/Dockerfile.affymetrix index 69983d365..4d6482afe 100644 --- a/workers/dockerfiles/Dockerfile.affymetrix +++ b/workers/dockerfiles/Dockerfile.affymetrix @@ -8,8 +8,8 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/affymetrix/renv.lock . -COPY common/R/renv_load.R . -RUN Rscript renv_load.R +COPY common/R/renv_load.R renv_load_affymetrix.R +RUN Rscript renv_load_affymetrix.R COPY workers/R/dependencies/affymetrix/install_ensg_pkgs.R . RUN Rscript install_ensg_pkgs.R diff --git a/workers/dockerfiles/Dockerfile.compendia b/workers/dockerfiles/Dockerfile.compendia index 23957c892..dd3352e8a 100644 --- a/workers/dockerfiles/Dockerfile.compendia +++ b/workers/dockerfiles/Dockerfile.compendia @@ -68,8 +68,8 @@ WORKDIR /home/user ENV R_LIBS=/usr/local/lib/R/site-library COPY workers/R/dependencies/compendia/renv.lock . -COPY common/R/renv_load.R . -RUN Rscript renv_load.R +COPY common/R/renv_load.R renv_load_compendia.R +RUN Rscript renv_load_compendia.R COPY workers/data_refinery_workers/requirements/compendia.txt requirements.txt RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 284203b35..9f48e98d4 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -8,16 +8,18 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/downloaders/renv.lock . -COPY common/R/renv_load.R . -RUN Rscript renv_load.R +COPY common/R/renv_load.R renv_load_downloaders.R +RUN Rscript renv_load_downloaders.R COPY workers/data_refinery_workers/downloaders/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Install Aspera. -RUN gpg --keyserver keyserver.ubuntu.com --recv-keys \ - 409B6B1796C275462A1703113804BB82D39DC0E3 \ - 7D2BAF1CF37B13E2069D6956105BD0E739499BDB && \ +# Disable IPv6 to avoid "Cannot assign requested address" error. +RUN mkdir ~/.gnupg/ && \ + echo "disable-ipv6" >> ~/.gnupg/dirmngr.conf && \ + gpg --keyserver keyserver.ubuntu.com --recv-keys \ + 409B6B1796C275462A1703113804BB82D39DC0E3 7D2BAF1CF37B13E2069D6956105BD0E739499BDB && \ curl -sSL https://get.rvm.io | bash -s stable --ruby --gems=aspera-cli USER user diff --git a/workers/dockerfiles/Dockerfile.illumina b/workers/dockerfiles/Dockerfile.illumina index ae00c06ca..5748788d9 100644 --- a/workers/dockerfiles/Dockerfile.illumina +++ b/workers/dockerfiles/Dockerfile.illumina @@ -8,8 +8,8 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/illumina/renv.lock . -COPY common/R/renv_load.R . -RUN Rscript renv_load.R +COPY common/R/renv_load.R renv_load_illumina.R +RUN Rscript renv_load_illumina.R COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt diff --git a/workers/dockerfiles/Dockerfile.no_op b/workers/dockerfiles/Dockerfile.no_op index f37a21ce4..a9ffe09dd 100644 --- a/workers/dockerfiles/Dockerfile.no_op +++ b/workers/dockerfiles/Dockerfile.no_op @@ -8,8 +8,8 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/no_op/renv.lock . -COPY common/R/renv_load.R . -RUN Rscript renv_load.R +COPY common/R/renv_load.R renv_load_no_op.R +RUN Rscript renv_load_no_op.R COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt diff --git a/workers/dockerfiles/Dockerfile.salmon b/workers/dockerfiles/Dockerfile.salmon index 6fb5885cb..ec2f49971 100644 --- a/workers/dockerfiles/Dockerfile.salmon +++ b/workers/dockerfiles/Dockerfile.salmon @@ -8,8 +8,8 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/salmon/renv.lock . -COPY common/R/renv_load.R . -RUN Rscript renv_load.R +COPY common/R/renv_load.R renv_load_salmon.R +RUN Rscript renv_load_salmon.R COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt diff --git a/workers/dockerfiles/Dockerfile.smasher b/workers/dockerfiles/Dockerfile.smasher index e04410251..077e6e110 100644 --- a/workers/dockerfiles/Dockerfile.smasher +++ b/workers/dockerfiles/Dockerfile.smasher @@ -8,8 +8,8 @@ SHELL ["/bin/bash", "-o", "pipefail", "-c"] WORKDIR /home/user COPY workers/R/dependencies/smasher/renv.lock . -COPY common/R/renv_load.R . -RUN Rscript renv_load.R +COPY common/R/renv_load.R renv_load_smasher.R +RUN Rscript renv_load_smasher.R COPY workers/data_refinery_workers/processors/requirements.txt . RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt From f4d7801f2255aa83e75efe1fdc60024f55c6cbf6 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 17 Aug 2023 09:54:30 -0700 Subject: [PATCH 201/220] Update deploy configuration - Set initial setup umask - Show additional docker builder information - Use versioned migrations image - Update downloaders docker image --- infrastructure/deploy_box_instance_data.sh | 2 +- scripts/common.sh | 3 ++- scripts/make_migrations.sh | 6 +++--- workers/dockerfiles/Dockerfile.downloaders | 4 ++-- 4 files changed, 8 insertions(+), 7 deletions(-) diff --git a/infrastructure/deploy_box_instance_data.sh b/infrastructure/deploy_box_instance_data.sh index dffe93ecf..5191b5e68 100644 --- a/infrastructure/deploy_box_instance_data.sh +++ b/infrastructure/deploy_box_instance_data.sh @@ -76,6 +76,6 @@ chown ubuntu:ubuntu /var/log/deploy.log # Checkout the repo onto the box. cd /home/ubuntu -umask 0022 +echo "umask 0022" > /home/ubuntu/.profile git clone https://github.com/AlexsLemonade/refinebio.git chown -R ubuntu:ubuntu refinebio diff --git a/scripts/common.sh b/scripts/common.sh index 8f9b5e59a..da6d677b0 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -112,8 +112,9 @@ EOF fi fi - echo "Using Docker builder $DOCKER_BUILDER." docker buildx use "$DOCKER_BUILDER" + echo "Using Docker builder $DOCKER_BUILDER:" + docker buildx inspect } update_docker_image() { diff --git a/scripts/make_migrations.sh b/scripts/make_migrations.sh index 807531569..523ede986 100755 --- a/scripts/make_migrations.sh +++ b/scripts/make_migrations.sh @@ -26,7 +26,7 @@ docker run \ --interactive \ --platform linux/amd64 \ --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ - "$DOCKERHUB_REPO/dr_migrations" \ + "$DOCKERHUB_REPO/dr_migrations:$SYSTEM_VERSION" \ python3 manage.py makemigrations data_refinery_common docker run \ @@ -34,7 +34,7 @@ docker run \ --env-file ../common/environments/local \ --platform linux/amd64 \ --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ - "$DOCKERHUB_REPO/dr_migrations" \ + "$DOCKERHUB_REPO/dr_migrations:$SYSTEM_VERSION" \ python3 manage.py migrate docker run \ @@ -42,5 +42,5 @@ docker run \ --env-file ../common/environments/local \ --platform linux/amd64 \ --volume "$script_directory/../common/data_refinery_common":/home/user/data_refinery_common \ - "$DOCKERHUB_REPO/dr_migrations" \ + "$DOCKERHUB_REPO/dr_migrations:$SYSTEM_VERSION" \ python3 manage.py createcachetable diff --git a/workers/dockerfiles/Dockerfile.downloaders b/workers/dockerfiles/Dockerfile.downloaders index 9f48e98d4..decf0cc80 100644 --- a/workers/dockerfiles/Dockerfile.downloaders +++ b/workers/dockerfiles/Dockerfile.downloaders @@ -16,14 +16,14 @@ RUN pip3 install --ignore-installed --no-cache-dir -r requirements.txt # Install Aspera. # Disable IPv6 to avoid "Cannot assign requested address" error. -RUN mkdir ~/.gnupg/ && \ +RUN mkdir -m 700 ~/.gnupg/ && \ echo "disable-ipv6" >> ~/.gnupg/dirmngr.conf && \ gpg --keyserver keyserver.ubuntu.com --recv-keys \ 409B6B1796C275462A1703113804BB82D39DC0E3 7D2BAF1CF37B13E2069D6956105BD0E739499BDB && \ curl -sSL https://get.rvm.io | bash -s stable --ruby --gems=aspera-cli USER user -RUN source /usr/local/rvm/scripts/rvm && \ +RUN . /usr/local/rvm/scripts/rvm && \ ascli conf ascp install USER root From 056fe9ef857d8faaabe68789ecca069b1c5a8802 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 17 Aug 2023 14:34:40 -0700 Subject: [PATCH 202/220] Fix instance data --- infrastructure/deploy_box_instance_data.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/infrastructure/deploy_box_instance_data.sh b/infrastructure/deploy_box_instance_data.sh index 5191b5e68..ba03c1ef3 100644 --- a/infrastructure/deploy_box_instance_data.sh +++ b/infrastructure/deploy_box_instance_data.sh @@ -76,6 +76,6 @@ chown ubuntu:ubuntu /var/log/deploy.log # Checkout the repo onto the box. cd /home/ubuntu -echo "umask 0022" > /home/ubuntu/.profile +echo "umask 0022" >> /home/ubuntu/.profile git clone https://github.com/AlexsLemonade/refinebio.git chown -R ubuntu:ubuntu refinebio From 1f5cd98e1ebdb5fe1db24bb80c9fe5c6b8ffda36 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Mon, 21 Aug 2023 11:06:28 -0400 Subject: [PATCH 203/220] add s3 to sra urls to check --- workers/data_refinery_workers/processors/salmon.py | 11 +++++------ 1 file changed, 5 insertions(+), 6 deletions(-) diff --git a/workers/data_refinery_workers/processors/salmon.py b/workers/data_refinery_workers/processors/salmon.py index 476deda1b..e87669e18 100644 --- a/workers/data_refinery_workers/processors/salmon.py +++ b/workers/data_refinery_workers/processors/salmon.py @@ -120,8 +120,11 @@ def _prepare_files(job_context: Dict) -> Dict: return job_context # Detect that this is an SRA file from the source URL - if ("ncbi.nlm.nih.gov" in job_context["original_files"][0].source_url) or ( - job_context["input_file_path"][-4:].upper() == ".SRA" + source_url = job_context["original_files"][0].source_url + if ( + ("ncbi.nlm.nih.gov" in source_url) + or ("sra-pub-run-odp.s3.amazonaws.com" in source_url) + or (job_context["input_file_path"][-4:].upper() == ".SRA") ): new_input_file_path = os.path.join(job_context["work_dir"], original_files[0].filename) shutil.copyfile(job_context["input_file_path"], new_input_file_path) @@ -461,7 +464,6 @@ def _find_or_download_index(job_context: Dict) -> Dict: def _run_tximport_for_experiment( job_context: Dict, experiment: Experiment, quant_files: List[ComputedFile] ) -> Dict: - # Download all the quant.sf fles for this experiment. Write all # their paths to a file so we can pass a path to that to # tximport.R rather than having to pass in one argument per @@ -688,10 +690,8 @@ def _run_salmon(job_context: Dict) -> Dict: # SRA files also get processed differently as we don't want to use fasterq-dump to extract # them to disk. if job_context.get("sra_input_file_path", None): - # Single reads if job_context["sra_num_reads"] == 1: - fifo = "/tmp/barney" os.mkfifo(fifo) @@ -715,7 +715,6 @@ def _run_salmon(job_context: Dict) -> Dict: ) # Paired are trickier else: - # Okay, for some reason I can't explain, this only works # in the temp directory, otherwise the `tee` part will # only output to one or the other of the streams From 2d631394eb87e326bc27b4b66b7287ceee4d3487 Mon Sep 17 00:00:00 2001 From: David Date: Mon, 21 Aug 2023 13:00:52 -0400 Subject: [PATCH 204/220] Update workers/data_refinery_workers/processors/salmon.py Co-authored-by: Arkadii Yakovets --- workers/data_refinery_workers/processors/salmon.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/workers/data_refinery_workers/processors/salmon.py b/workers/data_refinery_workers/processors/salmon.py index e87669e18..8344a3123 100644 --- a/workers/data_refinery_workers/processors/salmon.py +++ b/workers/data_refinery_workers/processors/salmon.py @@ -122,9 +122,9 @@ def _prepare_files(job_context: Dict) -> Dict: # Detect that this is an SRA file from the source URL source_url = job_context["original_files"][0].source_url if ( - ("ncbi.nlm.nih.gov" in source_url) - or ("sra-pub-run-odp.s3.amazonaws.com" in source_url) - or (job_context["input_file_path"][-4:].upper() == ".SRA") + "ncbi.nlm.nih.gov" in source_url + or "sra-pub-run-odp.s3.amazonaws.com" in source_url + or job_context["input_file_path"][-4:].upper() == ".SRA" ): new_input_file_path = os.path.join(job_context["work_dir"], original_files[0].filename) shutil.copyfile(job_context["input_file_path"], new_input_file_path) From 18a94691c79fa7df70c2e23103238c73ae251eff Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 22 Aug 2023 12:30:14 -0700 Subject: [PATCH 205/220] Add e2e tests troubleshooting changes - Increase batch launch template volume size from 300G to 500G - Fix random syntax/comments/style/typo issues --- .../models/original_file.py | 4 ++-- foreman/data_refinery_foreman/surveyor/geo.py | 2 +- foreman/tests/foreman/test_end_to_end.py | 5 +---- infrastructure/batch/compute.tf | 18 ++++++++---------- infrastructure/batch/launch_templates.tf | 2 +- scripts/format_batch_with_env.sh | 5 ++++- .../data_refinery_workers/downloaders/geo.py | 15 ++++++--------- .../data_refinery_workers/processors/utils.py | 2 +- workers/run_command.sh | 2 +- 9 files changed, 25 insertions(+), 30 deletions(-) diff --git a/common/data_refinery_common/models/original_file.py b/common/data_refinery_common/models/original_file.py index eba916929..49b541e21 100644 --- a/common/data_refinery_common/models/original_file.py +++ b/common/data_refinery_common/models/original_file.py @@ -31,7 +31,7 @@ class Meta: ] def __str__(self): - return "OriginalFile: " + self.get_display_name() + return f"OriginalFile: {self.get_display_name()}" # Managers objects = models.Manager() @@ -242,7 +242,7 @@ def needs_downloading(self, own_processor_id=None) -> bool: without getting processed. """ # If the file is downloaded and the file actually exists on disk, - # then it doens't need to be downloaded. + # then it doesn't need to be downloaded. if self.absolute_file_path and os.path.exists(self.absolute_file_path): # ok a file exists, if this file has an SHA1 ensure that it's the same existing_file_sha1 = calculate_sha1(self.absolute_file_path) diff --git a/foreman/data_refinery_foreman/surveyor/geo.py b/foreman/data_refinery_foreman/surveyor/geo.py index 347eb5693..94d0a6cf5 100644 --- a/foreman/data_refinery_foreman/surveyor/geo.py +++ b/foreman/data_refinery_foreman/surveyor/geo.py @@ -268,7 +268,7 @@ def create_experiment_and_samples_from_api( # Okay, here's the situation! # Sometimes, samples have a direct single representation for themselves. - # Othertimes, there is a single file with references to every sample in it. + # Other times, there is a single file with references to every sample in it. created_samples = [] for sample_accession_code, sample in gse.gsms.items(): try: diff --git a/foreman/tests/foreman/test_end_to_end.py b/foreman/tests/foreman/test_end_to_end.py index f6104bcfe..8eff635b9 100644 --- a/foreman/tests/foreman/test_end_to_end.py +++ b/foreman/tests/foreman/test_end_to_end.py @@ -59,9 +59,6 @@ EXPECTED_TOTAL_SAMPLES_COUNT = 191 - ARRAY_EXPRESS_SAMPLES_COUNT -print("Array Express samples count:", ARRAY_EXPRESS_SAMPLES_COUNT) -print("Expected total samples count:", EXPECTED_TOTAL_SAMPLES_COUNT) - EXPERIMENT_ACCESSION_CODES = MICROARRAY_ACCESSION_CODES + RNA_SEQ_ACCESSION_CODES TEST_DATA_BUCKET = "data-refinery-test-assets" @@ -361,7 +358,7 @@ def check_transcriptome_index(self): # Make sure that a processed file using our new transcriptome index is # similar enough to a reference file print( - "Now we are going to verify that the outputs of salmon look okay with" + "Now we are going to verify that the outputs of salmon look okay with " "the transcriptome index we generated." ) diff --git a/infrastructure/batch/compute.tf b/infrastructure/batch/compute.tf index 7e69fa3b8..f260867d6 100644 --- a/infrastructure/batch/compute.tf +++ b/infrastructure/batch/compute.tf @@ -1,5 +1,5 @@ -# This file creates the compute environments used by the queues -# The default environment is a 2 vCPU spot cluster +# This file creates the compute environments used by the queues. +# The default environment is a 2 vCPU spot cluster. locals { on_demand_environment_type = "EC2" @@ -26,7 +26,7 @@ resource "aws_batch_compute_environment" "data_refinery_workers" { max_vcpus = 32 min_vcpus = 0 - # standard launch template + # Standard launch template. launch_template { launch_template_id = aws_launch_template.data_refinery_worker.id } @@ -53,15 +53,13 @@ resource "aws_batch_compute_environment" "data_refinery_workers" { tags = var.default_tags } -# A separate queue for smasher jobs so they don't get stuck behind a -# large backlog. +# A separate queue for smasher jobs so they don't get stuck behind a large backlog. resource "aws_batch_compute_environment" "data_refinery_smasher" { compute_environment_name = "data-refinery-smasher-compute-${var.user}-${var.stage}" compute_resources { instance_role = aws_iam_instance_profile.ecs_instance_profile.arn instance_type = [ - # Just large enough to run one job at a time. We haven't had a - # backlog yet. + # Just large enough to run one job at a time. We haven't had a backlog yet. "m5.2xlarge", "r5.xlarge" ] @@ -72,7 +70,7 @@ resource "aws_batch_compute_environment" "data_refinery_smasher" { max_vcpus = 32 min_vcpus = 0 - # standard launch template + # Standard launch template. launch_template { launch_template_id = aws_launch_template.data_refinery_worker.id } @@ -105,7 +103,7 @@ resource "aws_batch_compute_environment" "data_refinery_compendia" { compute_resources { instance_role = aws_iam_instance_profile.ecs_instance_profile.arn instance_type = [ - # 976 GB RAM + # 976 GB RAM. "x1.16xlarge" ] @@ -116,7 +114,7 @@ resource "aws_batch_compute_environment" "data_refinery_compendia" { max_vcpus = 64 min_vcpus = 0 - # standard launch template + # Standard launch template. launch_template { launch_template_id = aws_launch_template.data_refinery_compendia.id } diff --git a/infrastructure/batch/launch_templates.tf b/infrastructure/batch/launch_templates.tf index 988c68ab7..a221c683e 100644 --- a/infrastructure/batch/launch_templates.tf +++ b/infrastructure/batch/launch_templates.tf @@ -16,7 +16,7 @@ resource "aws_launch_template" "data_refinery_worker" { device_name = "/dev/sdf" ebs { volume_type = "st1" - volume_size = 300 + volume_size = 500 encrypted = true delete_on_termination = true } diff --git a/scripts/format_batch_with_env.sh b/scripts/format_batch_with_env.sh index 21938e1e5..81d6e6169 100755 --- a/scripts/format_batch_with_env.sh +++ b/scripts/format_batch_with_env.sh @@ -213,6 +213,7 @@ if [ "$project" = "workers" ]; then echo "Made $FILEPATH" done echo "Made $output_dir/$OUTPUT_FILE" + # From https://unix.stackexchange.com/a/111517 elif (echo "$NO_RAM_JOB_FILES" | grep -Fqw "$OUTPUT_FILE"); then perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ @@ -220,6 +221,7 @@ if [ "$project" = "workers" ]; then >"$output_dir/$OUTPUT_FILE" \ 2>/dev/null echo "Made $output_dir/$OUTPUT_FILE" + else rams="2048 4096 8192 12288 16384 32768 65536" for r in $rams; do @@ -234,8 +236,8 @@ if [ "$project" = "workers" ]; then done fi done -elif [ "$project" = "surveyor" ]; then +elif [ "$project" = "surveyor" ]; then # Iterate over all the template files in the directory. for template in batch-job-templates/*.tpl.json; do template="$(basename "$template")" @@ -271,6 +273,7 @@ elif [ "$project" = "foreman" ]; then "$output_dir/environment" \ 2>/dev/null + elif [ "$project" = "api" ]; then perl -p -e 's/\$\{\{([^}]+)\}\}/defined $ENV{$1} ? $ENV{$1} : $&/eg' \ Date: Thu, 24 Aug 2023 17:07:21 -0700 Subject: [PATCH 206/220] Populate jobs worker_version field with the system version - Propagate SYSTEM_VERSION to api/foreman/worker environments - Update unit tests - Update end to end tests --- api/environment.tpl | 1 + api/tests/views/test_api_general.py | 5 +++++ foreman/batch-job-templates/surveyor.tpl.json | 1 + foreman/environment.tpl | 1 + foreman/tests/foreman/test_end_to_end.py | 11 +++++++---- infrastructure/deploy.sh | 11 ++++++----- .../foreman-server-instance-user-data.tpl.sh | 8 ++++---- workers/batch-job-templates/affy_to_pcl.tpl.json | 1 + workers/batch-job-templates/create_compendia.tpl.json | 1 + workers/batch-job-templates/create_qn_target.tpl.json | 1 + .../batch-job-templates/create_quantpendia.tpl.json | 1 + workers/batch-job-templates/downloader.tpl.json | 1 + workers/batch-job-templates/illumina.tpl.json | 1 + workers/batch-job-templates/janitor.tpl.json | 1 + workers/batch-job-templates/no_op.tpl.json | 1 + workers/batch-job-templates/qn_dispatcher.tpl.json | 1 + workers/batch-job-templates/qn_reference.tpl.json | 1 + workers/batch-job-templates/salmon.tpl.json | 1 + workers/batch-job-templates/smasher.tpl.json | 1 + .../batch-job-templates/transcriptome_index.tpl.json | 1 + workers/batch-job-templates/tximport.tpl.json | 1 + 21 files changed, 39 insertions(+), 13 deletions(-) diff --git a/api/environment.tpl b/api/environment.tpl index 2a02ed6e5..fa47b2032 100644 --- a/api/environment.tpl +++ b/api/environment.tpl @@ -1,3 +1,4 @@ +SYSTEM_VERSION=${{SYSTEM_VERSION}} DJANGO_SECRET_KEY=${{DJANGO_SECRET_KEY}} DJANGO_DEBUG=${{DJANGO_DEBUG}} DATABASE_PORT=${{DATABASE_PORT}} diff --git a/api/tests/views/test_api_general.py b/api/tests/views/test_api_general.py index fd817ccd2..39ebfd83d 100644 --- a/api/tests/views/test_api_general.py +++ b/api/tests/views/test_api_general.py @@ -1,4 +1,6 @@ import json +import os +from unittest import mock from django.core.cache import cache from django.urls import reverse @@ -31,6 +33,7 @@ from data_refinery_common.utils import get_env_variable API_VERSION = "v1" +SYSTEM_VERSION = "v2.0.0" class APITestCases(APITestCase): @@ -212,9 +215,11 @@ def tearDown(self): Sample.objects.all().delete() SampleAnnotation.objects.all().delete() + @mock.patch.dict(os.environ, {"SYSTEM_VERSION": SYSTEM_VERSION}) def test_all_endpoints(self): response = self.client.get(reverse("experiments", kwargs={"version": API_VERSION})) self.assertEqual(response.status_code, status.HTTP_200_OK) + self.assertTrue(response["X-Source-Revision"], SYSTEM_VERSION) self.assertEqual(response["X-Source-Revision"], get_env_variable("SYSTEM_VERSION")) cache.clear() diff --git a/foreman/batch-job-templates/surveyor.tpl.json b/foreman/batch-job-templates/surveyor.tpl.json index 6cb9567d7..53f04a163 100644 --- a/foreman/batch-job-templates/surveyor.tpl.json +++ b/foreman/batch-job-templates/surveyor.tpl.json @@ -26,6 +26,7 @@ } ], "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/foreman/environment.tpl b/foreman/environment.tpl index 8e253b0f6..86aab20ff 100644 --- a/foreman/environment.tpl +++ b/foreman/environment.tpl @@ -1,3 +1,4 @@ +SYSTEM_VERSION=${{SYSTEM_VERSION}} USER=${{USER}} STAGE=${{STAGE}} ENGAGEMENTBOT_WEBHOOK=${{ENGAGEMENTBOT_WEBHOOK}} diff --git a/foreman/tests/foreman/test_end_to_end.py b/foreman/tests/foreman/test_end_to_end.py index 8eff635b9..356dc9250 100644 --- a/foreman/tests/foreman/test_end_to_end.py +++ b/foreman/tests/foreman/test_end_to_end.py @@ -24,6 +24,7 @@ SampleComputedFileAssociation, SampleResultAssociation, ) +from data_refinery_common.utils import get_env_variable from data_refinery_foreman.foreman.management.commands.create_compendia import create_compendia from data_refinery_foreman.foreman.management.commands.create_quantpendia import create_quantpendia from data_refinery_foreman.foreman.management.commands.run_tximport import ( @@ -63,6 +64,8 @@ TEST_DATA_BUCKET = "data-refinery-test-assets" +SYSTEM_VERSION = get_env_variable("SYSTEM_VERSION") + def wait_for_job(job) -> bool: """Waits for a job and all of its retries.""" @@ -70,7 +73,6 @@ def wait_for_job(job) -> bool: is_done = False while not is_done: - if job.end_time is None and job.success is None: print(f"Polling {type(job).__name__}s. Currently waiting for job id: {job.id}") sleep(20) @@ -238,11 +240,8 @@ def test_all_the_things(self): purge_experiment(accession_code) self.process_experiments() - self.check_transcriptome_index() - self.create_qn_reference() - self.create_compendia() def process_experiments(self): @@ -315,6 +314,7 @@ def process_experiments(self): for downloader_job in downloader_jobs: self.assertTrue(wait_for_job(downloader_job)) + self.assertEqual(downloader_job.worker_version, SYSTEM_VERSION) processor_jobs = [] for sample in samples: @@ -334,6 +334,7 @@ def process_experiments(self): for processor_job in processor_jobs: self.assertTrue(wait_for_job(processor_job)) + self.assertEqual(downloader_job.worker_version, SYSTEM_VERSION) # Because SRR1583739 fails, the 26 samples from SRP047410 won't be processed EXPECTED_SAMPLE_FAILURES = 26 @@ -425,6 +426,7 @@ def create_qn_reference(self): self.assertIsNotNone(qn_job) self.assertTrue(wait_for_job(qn_job)) + self.assertEqual(qn_job.worker_version, SYSTEM_VERSION) def create_compendia(self): """Create both a compendium and a quantpendium""" @@ -433,3 +435,4 @@ def create_compendia(self): for job in compendia_jobs + quantpendia_jobs: self.assertTrue(wait_for_job(job)) + self.assertEqual(job.worker_version, SYSTEM_VERSION) diff --git a/infrastructure/deploy.sh b/infrastructure/deploy.sh index 2a49e0f14..3c9203187 100755 --- a/infrastructure/deploy.sh +++ b/infrastructure/deploy.sh @@ -109,6 +109,7 @@ fi # variable, which we then read in as json using the command line tool # `jq`, so that we can use them via bash. format_environment_variables() { + echo "SYSTEM_VERSION=$SYSTEM_VERSION" >>prod_env json_env_vars=$(terraform output -json environment_variables | jq -c '.[]') for row in $json_env_vars; do name=$(echo "$row" | jq -r ".name") @@ -203,7 +204,7 @@ if terraform output | grep -q 'No outputs found'; then terraform apply -var-file="environments/$env.tfvars" -auto-approve fi -# We have to do this once before the initial deploy.. +# We have to do this once before the initial deploy... rm -f prod_env format_environment_variables @@ -212,7 +213,7 @@ format_environment_variables if [[ -z $ran_init_build ]]; then # Open up ingress to AWS for Circle, stop jobs, migrate DB. - echo "Deploying with ingress.." + echo "Deploying with ingress..." # Output the plan for debugging deployments later. # Until terraform plan supports -var-file the plan is wrong. @@ -226,7 +227,7 @@ fi # `docker run` commands when running migrations. rm -f prod_env -# (cont'd) ..and once again after the update when this is re-run. +# (cont'd) ...and once again after the update when this is re-run. format_environment_variables # Make sure to clear out any old batch job templates since we @@ -273,7 +274,7 @@ docker pull --platform linux/amd64 "$DOCKERHUB_REPO/$FOREMAN_DOCKER_IMAGE" start_time=$(date +%s) diff=0 until pg_isready -d "$DATABASE_NAME" -h "$DATABASE_PUBLIC_HOST" -p "$DATABASE_PORT" -U "$DATABASE_USER" &>/dev/null || [ "$diff" -gt "900" ]; do - echo "Waiting for the pg_bouncer instance to come online ..." + echo "Waiting for the pg_bouncer instance to come online..." sleep 10 ((diff = $(date +%s) - start_time)) done @@ -327,7 +328,7 @@ terraform taint aws_instance.foreman_server_1 # tests can run and have access to the database since they don't use # test databases. if [ "$env" = "prod" ]; then - echo "Removing ingress.." + echo "Removing ingress..." rm ci_ingress.tf fi diff --git a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh index efc292163..0c509b04b 100644 --- a/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh +++ b/infrastructure/foreman-configuration/foreman-server-instance-user-data.tpl.sh @@ -34,11 +34,11 @@ docker run \\ --env ELASTICSEARCH_PORT="${elasticsearch_port}" \\ --env-file /home/ubuntu/environment \\ --interactive \\ - --log-driver=awslogs \\ + --log-driver awslogs \\ --log-opt awslogs-group="${log_group}" \\ --log-opt awslogs-region="${region}" \\ --log-opt awslogs-stream="log-stream-foreman-${user}-${stage}" \\ - --name=dr_foreman \\ + --name dr_foreman \\ --tty \\ --volume /tmp:/tmp \\ "${dockerhub_repo}/${foreman_docker_image}" \\ @@ -140,11 +140,11 @@ docker run \ --env DATABASE_USER="${database_user}" \ --env-file /home/ubuntu/environment \ --interactive \ - --log-driver=awslogs \ + --log-driver awslogs \ --log-opt awslogs-group="${log_group}" \ --log-opt awslogs-region="${region}" \ --log-opt awslogs-stream="log-stream-foreman-${user}-${stage}" \ - --name=job_filler \ + --name job_filler \ --tty \ --volume /tmp:/tmp \ "${dockerhub_repo}/${foreman_docker_image}" \ diff --git a/workers/batch-job-templates/affy_to_pcl.tpl.json b/workers/batch-job-templates/affy_to_pcl.tpl.json index c6594509f..bf0456737 100644 --- a/workers/batch-job-templates/affy_to_pcl.tpl.json +++ b/workers/batch-job-templates/affy_to_pcl.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/create_compendia.tpl.json b/workers/batch-job-templates/create_compendia.tpl.json index f8e97e70b..b5b3468c6 100644 --- a/workers/batch-job-templates/create_compendia.tpl.json +++ b/workers/batch-job-templates/create_compendia.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/create_qn_target.tpl.json b/workers/batch-job-templates/create_qn_target.tpl.json index eec119d06..dc7979b41 100644 --- a/workers/batch-job-templates/create_qn_target.tpl.json +++ b/workers/batch-job-templates/create_qn_target.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/create_quantpendia.tpl.json b/workers/batch-job-templates/create_quantpendia.tpl.json index 511400d9a..47107b10a 100644 --- a/workers/batch-job-templates/create_quantpendia.tpl.json +++ b/workers/batch-job-templates/create_quantpendia.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/downloader.tpl.json b/workers/batch-job-templates/downloader.tpl.json index 33b373806..64cf515c2 100644 --- a/workers/batch-job-templates/downloader.tpl.json +++ b/workers/batch-job-templates/downloader.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/illumina.tpl.json b/workers/batch-job-templates/illumina.tpl.json index e432c7777..f1dd73896 100644 --- a/workers/batch-job-templates/illumina.tpl.json +++ b/workers/batch-job-templates/illumina.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/janitor.tpl.json b/workers/batch-job-templates/janitor.tpl.json index 3ff27b738..1d178d160 100644 --- a/workers/batch-job-templates/janitor.tpl.json +++ b/workers/batch-job-templates/janitor.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/no_op.tpl.json b/workers/batch-job-templates/no_op.tpl.json index 1407e4084..249d2fd0c 100644 --- a/workers/batch-job-templates/no_op.tpl.json +++ b/workers/batch-job-templates/no_op.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/qn_dispatcher.tpl.json b/workers/batch-job-templates/qn_dispatcher.tpl.json index c1a32d793..abe0ec46e 100644 --- a/workers/batch-job-templates/qn_dispatcher.tpl.json +++ b/workers/batch-job-templates/qn_dispatcher.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/qn_reference.tpl.json b/workers/batch-job-templates/qn_reference.tpl.json index f1f53c716..bf69cf5f3 100644 --- a/workers/batch-job-templates/qn_reference.tpl.json +++ b/workers/batch-job-templates/qn_reference.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/salmon.tpl.json b/workers/batch-job-templates/salmon.tpl.json index ef38c3090..cd6e41114 100644 --- a/workers/batch-job-templates/salmon.tpl.json +++ b/workers/batch-job-templates/salmon.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/smasher.tpl.json b/workers/batch-job-templates/smasher.tpl.json index 8c7846b3b..973a11501 100644 --- a/workers/batch-job-templates/smasher.tpl.json +++ b/workers/batch-job-templates/smasher.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/transcriptome_index.tpl.json b/workers/batch-job-templates/transcriptome_index.tpl.json index 14892eca0..c671dd6f6 100644 --- a/workers/batch-job-templates/transcriptome_index.tpl.json +++ b/workers/batch-job-templates/transcriptome_index.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, diff --git a/workers/batch-job-templates/tximport.tpl.json b/workers/batch-job-templates/tximport.tpl.json index 7297e8d2a..5c52ad18d 100644 --- a/workers/batch-job-templates/tximport.tpl.json +++ b/workers/batch-job-templates/tximport.tpl.json @@ -28,6 +28,7 @@ ], "jobRoleArn": "${{WORKER_ROLE_ARN}}", "environment": [ + {"name": "SYSTEM_VERSION", "value": "${{SYSTEM_VERSION}}"}, {"name": "DJANGO_DEBUG", "value": "${{DJANGO_DEBUG}}"}, {"name": "DATABASE_NAME", "value": "${{DATABASE_NAME}}"}, {"name": "DATABASE_USER", "value": "${{DATABASE_USER}}"}, From 4bdc42ed6df570cc68cd37c61595cebed6931027 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 25 Aug 2023 08:09:35 -0700 Subject: [PATCH 207/220] Update API tests --- api/tests/views/test_api_general.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/api/tests/views/test_api_general.py b/api/tests/views/test_api_general.py index 39ebfd83d..559bb2080 100644 --- a/api/tests/views/test_api_general.py +++ b/api/tests/views/test_api_general.py @@ -219,7 +219,7 @@ def tearDown(self): def test_all_endpoints(self): response = self.client.get(reverse("experiments", kwargs={"version": API_VERSION})) self.assertEqual(response.status_code, status.HTTP_200_OK) - self.assertTrue(response["X-Source-Revision"], SYSTEM_VERSION) + self.assertEqual(response["X-Source-Revision"], SYSTEM_VERSION) self.assertEqual(response["X-Source-Revision"], get_env_variable("SYSTEM_VERSION")) cache.clear() From 275c1ee73eb7fa36707b55f8f63ad9e0b63be5d8 Mon Sep 17 00:00:00 2001 From: David Mejia Date: Fri, 25 Aug 2023 15:31:30 -0400 Subject: [PATCH 208/220] check if SHORT and LONG indices exist --- common/data_refinery_common/rna_seq.py | 15 +++++++++------ 1 file changed, 9 insertions(+), 6 deletions(-) diff --git a/common/data_refinery_common/rna_seq.py b/common/data_refinery_common/rna_seq.py index a9aee68cc..dc9137bb9 100644 --- a/common/data_refinery_common/rna_seq.py +++ b/common/data_refinery_common/rna_seq.py @@ -28,13 +28,16 @@ EARLY_TXIMPORT_MIN_PERCENT = 0.80 -def get_latest_organism_index(organism): +def get_latest_organism_indices(organism): # Salmon version gets saved as what salmon outputs, which includes this prefix. current_salmon_version = "salmon " + get_env_variable("SALMON_VERSION", "0.13.1") + indices = OrganismIndex.objects.filter( + salmon_version=current_salmon_version, organism=organism + ).order_by("-created_at") + return ( - OrganismIndex.objects.filter(salmon_version=current_salmon_version, organism=organism) - .order_by("-created_at") - .first() + indices.filter(index_type="TRANSCRIPTOME_LONG").first(), + indices.filter(index_type="TRANSCRIPTOME_SHORT").first(), ) @@ -45,7 +48,7 @@ def get_tximport_inputs_if_eligible(experiment: Experiment, is_tximport_job: boo """ organism_indices = {} for organism in experiment.organisms.all(): - organism_indices[organism.id] = get_latest_organism_index(organism) + organism_indices[organism.id] = get_latest_organism_indices(organism) good_quant_files = [] num_unprocessable_samples = 0 @@ -58,7 +61,7 @@ def get_tximport_inputs_if_eligible(experiment: Experiment, is_tximport_job: boo and sample.most_recent_quant_file.s3_key is not None ): sample_organism_index = sample.most_recent_quant_file.result.organism_index - if sample_organism_index == organism_indices[sample.organism.id]: + if sample_organism_index in organism_indices[sample.organism.id]: good_quant_files.append(sample.most_recent_quant_file) num_quantified = len(good_quant_files) From 57ae90e961a8356b70d275caa160b656cf42939c Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 25 Aug 2023 15:10:25 -0700 Subject: [PATCH 209/220] Update batch job definitions clean up process - Narrow down expected exceptions for batch job queue deletion - Use nextToken for 100+ duplicate entries cases - Add `create_qn_target.json` to the list of fixed RAM jobs --- infrastructure/delete_batch_job_queue.py | 11 ++++-- infrastructure/deploy.sh | 2 +- .../deregister_batch_job_definitions.py | 39 ++++++++++++------- infrastructure/destroy_terraform.sh | 2 +- scripts/format_batch_with_env.sh | 2 +- 5 files changed, 35 insertions(+), 21 deletions(-) diff --git a/infrastructure/delete_batch_job_queue.py b/infrastructure/delete_batch_job_queue.py index 55602cc94..911486148 100644 --- a/infrastructure/delete_batch_job_queue.py +++ b/infrastructure/delete_batch_job_queue.py @@ -2,19 +2,22 @@ from time import sleep import boto3 +from botocore.exceptions import ClientError -AWS_REGION = os.environ["AWS_REGION"] AWS_BATCH_QUEUE_ALL_NAMES = os.environ["REFINEBIO_JOB_QUEUE_ALL_NAMES"].split(",") -batch = boto3.client("batch", region_name=AWS_REGION) +batch = boto3.client("batch", region_name=os.environ["AWS_REGION"]) # First disable each job queue. for batch_queue_name in AWS_BATCH_QUEUE_ALL_NAMES: try: batch.update_job_queue(jobQueue=batch_queue_name, state="DISABLED") - except Exception as e: + except ClientError as e: # If the job queue doesn't exist, that's cool, we were trying to delete it anyway. - pass + if str(e).endswith(" does not exist."): + pass + else: + raise e # Then wait for each one to be disabled so it can be deleted. for batch_queue_name in AWS_BATCH_QUEUE_ALL_NAMES: diff --git a/infrastructure/deploy.sh b/infrastructure/deploy.sh index 3c9203187..5f4808bd7 100755 --- a/infrastructure/deploy.sh +++ b/infrastructure/deploy.sh @@ -256,7 +256,7 @@ python3 delete_batch_job_queue.py python3 deregister_batch_job_definitions.py # Re-register Batch jobs (skip those that end in .tpl) -echo "Registering new job specifications.." +echo "Registering new job specifications..." export AWS_DEFAULT_REGION=$AWS_REGION # SC2010: Don't use ls | grep. Use a glob or a for loop with a condition to allow non-alphanumeric filenames. # We are using a glob, but we want to limit it to a specific directory. Seems like an over aggressive check. diff --git a/infrastructure/deregister_batch_job_definitions.py b/infrastructure/deregister_batch_job_definitions.py index 552175d21..7d9d945c6 100644 --- a/infrastructure/deregister_batch_job_definitions.py +++ b/infrastructure/deregister_batch_job_definitions.py @@ -2,25 +2,36 @@ import boto3 -AWS_REGION = os.environ["AWS_REGION"] - -batch = boto3.client("batch", region_name=AWS_REGION) +batch = boto3.client("batch", region_name=os.environ["AWS_REGION"]) # TODO: stop repeating this construction everywhere. Just set it once somewhere. JOB_DEFINITION_PREFIX = os.environ["USER"] + "_" + os.environ["STAGE"] + "_" -job_definition_files = os.listdir("batch-job-templates") - -job_definition_list = list( - {JOB_DEFINITION_PREFIX + job_def.upper().split(".")[0] for job_def in job_definition_files} +job_names = ( + JOB_DEFINITION_PREFIX + batch_job_template.upper().split(".")[0] + for batch_job_template in os.listdir("batch-job-templates") ) +nextToken = "" # Have to go one by one because providing a list of job names doesn't work: # https://github.com/boto/boto3/issues/2908 -for job_definition in job_definition_list: - job_definitions = batch.describe_job_definitions( - jobDefinitionName=job_definition, status="ACTIVE" - ) - # There can be multiple revisions per job definition. We want them all gone. - for job_definition_revision in job_definitions["jobDefinitions"]: - batch.deregister_job_definition(jobDefinition=job_definition_revision["jobDefinitionArn"]) +for job_name in sorted(job_names): + while True: + data = { + "jobDefinitionName": job_name, + "maxResults": 100, + "status": "ACTIVE", + } + if nextToken: + data["nextToken"] = nextToken + + response = batch.describe_job_definitions(**data) + nextToken = response.get("nextToken", "") + + job_definitions = response.get("jobDefinitions") + if not job_definitions: + break + + # There can be multiple revisions per job definition. We want them all gone. + for job_definition in job_definitions: + batch.deregister_job_definition(jobDefinition=job_definition["jobDefinitionArn"]) diff --git a/infrastructure/destroy_terraform.sh b/infrastructure/destroy_terraform.sh index 4329cf885..24b1b98df 100755 --- a/infrastructure/destroy_terraform.sh +++ b/infrastructure/destroy_terraform.sh @@ -71,7 +71,7 @@ fi terraform destroy -var-file="environments/$env.tfvars" -# These aren't managed by terraform so we have to deregistr them manually.. +# These aren't managed by terraform so we have to deregistr them manually. export USER="$TF_VAR_user" export STAGE="$TF_VAR_stage" export AWS_REGION="$TF_VAR_region" diff --git a/scripts/format_batch_with_env.sh b/scripts/format_batch_with_env.sh index 81d6e6169..accc7cc83 100755 --- a/scripts/format_batch_with_env.sh +++ b/scripts/format_batch_with_env.sh @@ -170,7 +170,7 @@ if [ "$project" = "workers" ]; then FILETYPE=".json" OUTPUT_FILE="$OUTPUT_BASE$FILETYPE" - NO_RAM_JOB_FILES="smasher.json tximport.json qn_dispatcher.json janitor.json" + NO_RAM_JOB_FILES="create_qn_target.json smasher.json tximport.json qn_dispatcher.json janitor.json" if [ "$OUTPUT_FILE" = "downloader.json" ]; then rams="1024 4096 16384" From 54afa48292107b7a0b8ccd43ef1822138f65c5fe Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Fri, 25 Aug 2023 16:48:26 -0700 Subject: [PATCH 210/220] Increase launch templates /dev/xvda volume size --- infrastructure/batch/launch_templates.tf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/infrastructure/batch/launch_templates.tf b/infrastructure/batch/launch_templates.tf index a221c683e..cac79476d 100644 --- a/infrastructure/batch/launch_templates.tf +++ b/infrastructure/batch/launch_templates.tf @@ -6,7 +6,7 @@ resource "aws_launch_template" "data_refinery_worker" { block_device_mappings { device_name = "/dev/xvda" ebs { - volume_size = 64 + volume_size = 128 encrypted = true delete_on_termination = true } @@ -43,7 +43,7 @@ resource "aws_launch_template" "data_refinery_compendia" { block_device_mappings { device_name = "/dev/xvda" ebs { - volume_size = 64 + volume_size = 128 encrypted = true delete_on_termination = true } From 7844f1e601d841e80bd5f9d26bcebc66cfb6fd9f Mon Sep 17 00:00:00 2001 From: Jaclyn Taroni <19534205+jaclyn-taroni@users.noreply.github.com> Date: Mon, 28 Aug 2023 12:49:37 -0400 Subject: [PATCH 211/220] Add new airway pathogens to organism-strain mapping file --- config/organism_strain_mapping.csv | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/config/organism_strain_mapping.csv b/config/organism_strain_mapping.csv index 7d8a7a3f6..7ec9ce5dd 100644 --- a/config/organism_strain_mapping.csv +++ b/config/organism_strain_mapping.csv @@ -2,3 +2,8 @@ organism,ensembl_division,strain,assembly PSEUDOMONAS_AERUGINOSA,EnsemblBacteria,PAO1,ASM676v1 CANDIDA_ALBICANS,EnsemblFungi,SC5314,Cand_albi_SC5314_V4 ESCHERICHIA_COLI,EnsemblBacteria,ATCC 25922,Escherichia coli ATCC 25922 +STAPHYLOCOCCUS_AUREUS,EnsemblBacteria,USA300_FPR3757,ASM1346v1 +STREPTOCOCCUS_PNEUMONIAE,EnsemblBacteria,TIGR4 ATCC BAA-334,ASM688v1 +STENOTROPHOMONAS_MALTOPHILIA,EnsemblBacteria,K279a,ASM7248v1 +ASPERGILLUS_FUMIGATUS,EnsemblFungi,Af293,ASM265v1 +KLEBSIELLA_PNEUMONIAE,EnsemblBacteria,ATCC 700721 MGH 78578,ASM1630v1 From de3c21466c6c2e78c712c59d7f6cdc923924cc67 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 28 Aug 2023 12:01:16 -0700 Subject: [PATCH 212/220] Rename illumina job template Make it consistent with the job name --- .../{illumina.tpl.json => illumina_to_pcl.tpl.json} | 0 1 file changed, 0 insertions(+), 0 deletions(-) rename workers/batch-job-templates/{illumina.tpl.json => illumina_to_pcl.tpl.json} (100%) diff --git a/workers/batch-job-templates/illumina.tpl.json b/workers/batch-job-templates/illumina_to_pcl.tpl.json similarity index 100% rename from workers/batch-job-templates/illumina.tpl.json rename to workers/batch-job-templates/illumina_to_pcl.tpl.json From e08469bbdd9a3dd2cd11343c19befc0e6de2b9fc Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Mon, 28 Aug 2023 17:59:19 -0700 Subject: [PATCH 213/220] Remove remote docker builder for Github tests Move Foreman tests to larger runners. --- .github/workflows/config.yml | 2 +- scripts/common.sh | 42 ++++++------------------------------ 2 files changed, 8 insertions(+), 36 deletions(-) diff --git a/.github/workflows/config.yml b/.github/workflows/config.yml index 41e956897..851201cf7 100644 --- a/.github/workflows/config.yml +++ b/.github/workflows/config.yml @@ -292,7 +292,7 @@ jobs: IMAGES: migrations foreman needs: - test_api - runs-on: ubuntu-latest + runs-on: ubuntu-latest-m steps: - uses: actions/checkout@v3 diff --git a/scripts/common.sh b/scripts/common.sh index da6d677b0..14931c6df 100755 --- a/scripts/common.sh +++ b/scripts/common.sh @@ -75,41 +75,13 @@ run_tests_with_coverage() { # Set the builder as currently used. set_up_docker_builder() { if [ -z "$DOCKER_BUILDER" ]; then - echo "Setting up refine.bio Docker builder." - - if test "$GITHUB_ACTION"; then - echo "$INSTANCE_SSH_KEY" >infrastructure/data-refinery-key.pem - chmod 600 infrastructure/data-refinery-key.pem - - # shellcheck disable=SC2046 - eval $(ssh-agent) - ssh-add infrastructure/data-refinery-key.pem - - if [ ! -d ~/.ssh ]; then - mkdir -m 700 ~/.ssh - fi - cat >~/.ssh/config </dev/null || - true - else - DOCKER_BUILDER="refinebio_local_builder" - echo "Creating Docker builder $DOCKER_BUILDER." - docker buildx create \ - --driver=docker-container \ - --name="$DOCKER_BUILDER" \ - --platform=linux/amd64 2>/dev/null || - true - fi + DOCKER_BUILDER="refinebio_local_builder" + echo "Creating Docker builder $DOCKER_BUILDER." + docker buildx create \ + --driver=docker-container \ + --name="$DOCKER_BUILDER" \ + --platform=linux/amd64 2>/dev/null || + true fi docker buildx use "$DOCKER_BUILDER" From 8462803a0446c5fe488904af8930f3f0872f9664 Mon Sep 17 00:00:00 2001 From: Dom <97384583+tosemml@users.noreply.github.com> Date: Tue, 29 Aug 2023 01:30:19 -0700 Subject: [PATCH 214/220] use list comp --- foreman/data_refinery_foreman/foreman/utils.py | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/foreman/data_refinery_foreman/foreman/utils.py b/foreman/data_refinery_foreman/foreman/utils.py index 681424780..93212c3f4 100644 --- a/foreman/data_refinery_foreman/foreman/utils.py +++ b/foreman/data_refinery_foreman/foreman/utils.py @@ -66,10 +66,10 @@ def check_hung_jobs(object_list): batch_jobs = batch.describe_jobs(jobs=job_ids)["jobs"] running_job_batch_ids = {job["jobId"] for job in batch_jobs if job["status"] == "RUNNING"} - - for job in page: - if job.batch_job_id and job.batch_job_id not in running_job_batch_ids: - hung_jobs.append(job) + hung_jobs += [ + job for job in page + if job.batch_job_id and job.batch_job_id not in running_job_batch_ids + ] return hung_jobs From 3e102e4447b343c598659aa9ca04287423ddca80 Mon Sep 17 00:00:00 2001 From: Dom <97384583+tosemml@users.noreply.github.com> Date: Tue, 29 Aug 2023 01:32:08 -0700 Subject: [PATCH 215/220] use list comp --- foreman/data_refinery_foreman/foreman/utils.py | 5 +---- 1 file changed, 1 insertion(+), 4 deletions(-) diff --git a/foreman/data_refinery_foreman/foreman/utils.py b/foreman/data_refinery_foreman/foreman/utils.py index 93212c3f4..1460f0ec0 100644 --- a/foreman/data_refinery_foreman/foreman/utils.py +++ b/foreman/data_refinery_foreman/foreman/utils.py @@ -91,9 +91,6 @@ def check_lost_jobs(object_list): # have yet gotten to that point. ignore = ["SUBMITTED", "PENDING", "RUNNABLE", "STARTING", "RUNNING"] ignore_job_batch_ids = {job["jobId"] for job in batch_jobs if job["status"] in ignore} - - for job in page: - if not job.batch_job_id or job.batch_job_id not in ignore_job_batch_ids: - lost_jobs.append(job) + lost_jobs += [job for job in page if not job.batch_job_id or job.batch_job_id not in ignore_job_batch_ids] return lost_jobs From e31b4fae840126099b469aa3b9d0f892f3c34b1f Mon Sep 17 00:00:00 2001 From: Dom <97384583+tosemml@users.noreply.github.com> Date: Tue, 29 Aug 2023 01:35:39 -0700 Subject: [PATCH 216/220] indentation --- foreman/data_refinery_foreman/foreman/utils.py | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/foreman/data_refinery_foreman/foreman/utils.py b/foreman/data_refinery_foreman/foreman/utils.py index 1460f0ec0..c88be2998 100644 --- a/foreman/data_refinery_foreman/foreman/utils.py +++ b/foreman/data_refinery_foreman/foreman/utils.py @@ -91,6 +91,9 @@ def check_lost_jobs(object_list): # have yet gotten to that point. ignore = ["SUBMITTED", "PENDING", "RUNNABLE", "STARTING", "RUNNING"] ignore_job_batch_ids = {job["jobId"] for job in batch_jobs if job["status"] in ignore} - lost_jobs += [job for job in page if not job.batch_job_id or job.batch_job_id not in ignore_job_batch_ids] + lost_jobs += [ + job for job in page + if not job.batch_job_id or job.batch_job_id not in ignore_job_batch_ids + ] return lost_jobs From 123c59066af09e9bb99463d9c98d257b4f0e2e79 Mon Sep 17 00:00:00 2001 From: Dom <97384583+tosemml@users.noreply.github.com> Date: Tue, 29 Aug 2023 01:36:57 -0700 Subject: [PATCH 217/220] remove space --- foreman/data_refinery_foreman/foreman/utils.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/foreman/data_refinery_foreman/foreman/utils.py b/foreman/data_refinery_foreman/foreman/utils.py index c88be2998..c368f647a 100644 --- a/foreman/data_refinery_foreman/foreman/utils.py +++ b/foreman/data_refinery_foreman/foreman/utils.py @@ -67,7 +67,7 @@ def check_hung_jobs(object_list): running_job_batch_ids = {job["jobId"] for job in batch_jobs if job["status"] == "RUNNING"} hung_jobs += [ - job for job in page + job for job in page if job.batch_job_id and job.batch_job_id not in running_job_batch_ids ] @@ -92,7 +92,7 @@ def check_lost_jobs(object_list): ignore = ["SUBMITTED", "PENDING", "RUNNABLE", "STARTING", "RUNNING"] ignore_job_batch_ids = {job["jobId"] for job in batch_jobs if job["status"] in ignore} lost_jobs += [ - job for job in page + job for job in page if not job.batch_job_id or job.batch_job_id not in ignore_job_batch_ids ] From 09dd604c88229e8b2544620389bc6de84e4f3770 Mon Sep 17 00:00:00 2001 From: Jaclyn Taroni <19534205+jaclyn-taroni@users.noreply.github.com> Date: Tue, 29 Aug 2023 10:41:43 -0400 Subject: [PATCH 218/220] Update supported_microarray_platforms.csv --- config/supported_microarray_platforms.csv | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/config/supported_microarray_platforms.csv b/config/supported_microarray_platforms.csv index eceb027c1..e1ba3db1c 100644 --- a/config/supported_microarray_platforms.csv +++ b/config/supported_microarray_platforms.csv @@ -35,7 +35,7 @@ clariomdhuman,A-GEOD-22844,y clariomshumanht,GPL24324,y clariomshuman,GPL23159,y clariomsmouse,GPL23038,y -clariomsmouse,GPL24242,y +clariomsmouseht,GPL24242,y clariomsrat,GPL23040,y drogene10st,GPL20842,y drogene10st,GPL18094,y From 6aba26483be5df38a0569fe061b9ebb235415bc0 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Tue, 29 Aug 2023 11:02:10 -0700 Subject: [PATCH 219/220] Run pre-commit --- foreman/data_refinery_foreman/foreman/utils.py | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/foreman/data_refinery_foreman/foreman/utils.py b/foreman/data_refinery_foreman/foreman/utils.py index c368f647a..f4bb6b431 100644 --- a/foreman/data_refinery_foreman/foreman/utils.py +++ b/foreman/data_refinery_foreman/foreman/utils.py @@ -67,7 +67,8 @@ def check_hung_jobs(object_list): running_job_batch_ids = {job["jobId"] for job in batch_jobs if job["status"] == "RUNNING"} hung_jobs += [ - job for job in page + job + for job in page if job.batch_job_id and job.batch_job_id not in running_job_batch_ids ] @@ -92,7 +93,8 @@ def check_lost_jobs(object_list): ignore = ["SUBMITTED", "PENDING", "RUNNABLE", "STARTING", "RUNNING"] ignore_job_batch_ids = {job["jobId"] for job in batch_jobs if job["status"] in ignore} lost_jobs += [ - job for job in page + job + for job in page if not job.batch_job_id or job.batch_job_id not in ignore_job_batch_ids ] From 1c833d3e6cc2a191a5f4f97d42ba7b88b00ec358 Mon Sep 17 00:00:00 2001 From: Arkadii Yakovets Date: Thu, 31 Aug 2023 09:34:24 -0700 Subject: [PATCH 220/220] Update smasher email templates: use new ALSF address --- .../data_refinery_workers/processors/smasher_email.html | 7 +++---- .../processors/smasher_email.min.html | 2 +- .../processors/smasher_email_error.html | 7 +++---- .../processors/smasher_email_error.min.html | 2 +- 4 files changed, 8 insertions(+), 10 deletions(-) diff --git a/workers/data_refinery_workers/processors/smasher_email.html b/workers/data_refinery_workers/processors/smasher_email.html index f5ba5710d..d3035bc4d 100644 --- a/workers/data_refinery_workers/processors/smasher_email.html +++ b/workers/data_refinery_workers/processors/smasher_email.html @@ -1840,10 +1840,9 @@

- Alex's Lemonade Stand Foundation
- 111 Presidential Blvd, Suite 203, Bala - Cynwyd, PA 19004 + Alex’s Lemonade Stand Foundation for Childhood Cancer
+ 333 E. Lancaster Ave, #414, Wynnewood, PA 19096 USA +

diff --git a/workers/data_refinery_workers/processors/smasher_email.min.html b/workers/data_refinery_workers/processors/smasher_email.min.html index a74f8638a..b2ddd7aac 100644 --- a/workers/data_refinery_workers/processors/smasher_email.min.html +++ b/workers/data_refinery_workers/processors/smasher_email.min.html @@ -1 +1 @@ -Title




Hot off the presses!


Your dataset is ready to be downloaded.


Download Now

Terms of Use

Alex's Lemonade Stand Foundation
111 Presidential Blvd, Suite 203, Bala Cynwyd, PA 19004


+Title




Hot off the presses!


Your dataset is ready to be downloaded.


Download Now

Terms of Use

Alex’s Lemonade Stand Foundation for Childhood Cancer
333 E. Lancaster Ave, #414, Wynnewood, PA 19096 USA


diff --git a/workers/data_refinery_workers/processors/smasher_email_error.html b/workers/data_refinery_workers/processors/smasher_email_error.html index 2f43801a1..9cb43092a 100644 --- a/workers/data_refinery_workers/processors/smasher_email_error.html +++ b/workers/data_refinery_workers/processors/smasher_email_error.html @@ -1847,10 +1847,9 @@

- Alex's Lemonade Stand Foundation
- 111 Presidential Blvd, Suite 203, Bala - Cynwyd, PA 19004 + Alex’s Lemonade Stand Foundation for Childhood Cancer
+ 333 E. Lancaster Ave, #414, Wynnewood, PA 19096 USA +

diff --git a/workers/data_refinery_workers/processors/smasher_email_error.min.html b/workers/data_refinery_workers/processors/smasher_email_error.min.html index 915cc0709..94101663b 100644 --- a/workers/data_refinery_workers/processors/smasher_email_error.min.html +++ b/workers/data_refinery_workers/processors/smasher_email_error.min.html @@ -1 +1 @@ -Title




We were unable to process your dataset


We have been notified and are looking into this problem. We apologize for the inconvenience. You can try downloading your dataset after sometime here.

If the error persists, feel free to file an issue on Github or email us at requests@ccdatalab.org with the error details below:

REPLACE_ERROR_TEXT


Terms of Use

Alex's Lemonade Stand Foundation
111 Presidential Blvd, Suite 203, Bala Cynwyd, PA 19004


+Title




We were unable to process your dataset


We have been notified and are looking into this problem. We apologize for the inconvenience. You can try downloading your dataset after sometime here.

If the error persists, feel free to file an issue on Github or email us at requests@ccdatalab.org with the error details below:

REPLACE_ERROR_TEXT


Terms of Use

Alex’s Lemonade Stand Foundation for Childhood Cancer
333 E. Lancaster Ave, #414, Wynnewood, PA 19096 USA