What is the file of Snakefile?

[leegene92]

Hello, I am trying to use MarkerScan to check cobiont of the assembly. But from the manual, the command is
singularity run markerscan_latest.sif snakemake --cores $threads --use-conda --conda-prefix /opt/conda/ -s /MarkerScanPipeline/Snakefile --configfile $configfile
What is the Snakefile here? I tried it several times, it always suggest no Snakefile found.
Meanwhile, in the config file, the first one is "reads: zipped fasta read file".
Is it a PacBio hifi fasta reads, but not fastq?
Many thanks.