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Here, we show how to design and perform BFG-PCA experiments.

======================This part is still under construction =================

Table of contents

1. Designing the space

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2. Prepare the barcoded expression vectors

3. Estimate the required experiment scale

4. BFG-PCA screening

   • Haploid cell culture
   • En masse mating
   • Diploid selection
   • Selection
   • Dox induction
   • Plasmid prep
   • Library PCR
   • Sequencing run
   • Data analysis
     • Barcode calling
     • Normalization
     • Performance measure
     • Visualization

Note:

We are happy to share an example of the sequencing data from our publication to execute these codes. In that case please send me an email at daney[at]sfc.keio.ac.jp . (We initially tried to submit the data to SRA, but their scope was out of the barcode amplicon sequencing.)