diff --git a/tracer/test/test-applications/integrations/Samples.AWS.S3/AsyncHelpers.cs b/tracer/test/test-applications/integrations/Samples.AWS.S3/AsyncHelpers.cs
index 97f77904a844..a405d80fa025 100644
--- a/tracer/test/test-applications/integrations/Samples.AWS.S3/AsyncHelpers.cs
+++ b/tracer/test/test-applications/integrations/Samples.AWS.S3/AsyncHelpers.cs
@@ -138,7 +138,7 @@ string content
                 Key = objectKey,
                 UploadId = uploadId,
                 PartNumber = partNumber,
-                InputStream = memoryStream
+                InputStream = memoryStream,
             };
 
             var response = await s3Client.UploadPartAsync(uploadRequest);
diff --git a/tracer/test/test-applications/integrations/Samples.AWS.S3/Program.cs b/tracer/test/test-applications/integrations/Samples.AWS.S3/Program.cs
index c92f87d6f05c..c667f9772cd6 100644
--- a/tracer/test/test-applications/integrations/Samples.AWS.S3/Program.cs
+++ b/tracer/test/test-applications/integrations/Samples.AWS.S3/Program.cs
@@ -18,6 +18,10 @@ private static async Task Main(string[] args)
 
         private static AmazonS3Client GetAmazonS3Client()
         {
+            // Fixes localstack breaking change in AWSSSDK.S3 versions 3.7.412.0 and above.
+            // https://github.com/aws/aws-sdk-net/issues/3610
+            Environment.SetEnvironmentVariable("AWS_REQUEST_CHECKSUM_CALCULATION", "WHEN_REQUIRED");
+
             if (Environment.GetEnvironmentVariable("AWS_ACCESSKEY") is string accessKey &&
                 Environment.GetEnvironmentVariable("AWS_SECRETKEY") is string secretKey &&
                 Environment.GetEnvironmentVariable("AWS_REGION") is string region)
diff --git a/tracer/test/test-applications/integrations/Samples.AWS.S3/SyncHelpers.cs b/tracer/test/test-applications/integrations/Samples.AWS.S3/SyncHelpers.cs
index 693dae33aace..8c72d8081343 100644
--- a/tracer/test/test-applications/integrations/Samples.AWS.S3/SyncHelpers.cs
+++ b/tracer/test/test-applications/integrations/Samples.AWS.S3/SyncHelpers.cs
@@ -141,7 +141,7 @@ string content
                 Key = objectKey,
                 UploadId = uploadId,
                 PartNumber = partNumber,
-                InputStream = memoryStream
+                InputStream = memoryStream,
             };
 
             var response = s3Client.UploadPart(request);