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error in adding specific sequences when building kraken2-custom database #913

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alarasutcu opened this issue Feb 13, 2025 · 0 comments
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@alarasutcu
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Hi,

I am trying to build a custom database. I am having some issues with being able to add my sequences to the database.

I am using specified line to add ~38k viral sequences to the database,
kraken2-build --add-to-library <path_to_sequences.fna> --db <kraken_path> but I am not certain that they are being added.

When it comes time to building the database kraken2-build --build --db <kraken_path> it is saying that 0 sequences were processed.

Image

And when I inspect the output this is what I see:

Image

Do you have any suggestions of what to do? Also, it would be helpful to understand the limitations of what can be added to the database?

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