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If the z position is far away from the first Step in a Protocol, the time it takes to get to the correct focus is added to the first image start time and thus the second image comes too soon afterwards. The first image "clock" starts with the initiation of the moving to the right focus, not when the first image is acquired. This is most likely also occurring when a microplate Protocol "parks" at the last well imaged instead of the next to be imaged well between intervals. In this case the initiation of the XY translation will be the t=0 rather than the first image acquisition. So the first well of a multi-well Protocol is slightly different in timing, not important if the interval is 8 hours but at 6 seconds it's very noticeable.
The text was updated successfully, but these errors were encountered:
If the z position is far away from the first Step in a Protocol, the time it takes to get to the correct focus is added to the first image start time and thus the second image comes too soon afterwards. The first image "clock" starts with the initiation of the moving to the right focus, not when the first image is acquired. This is most likely also occurring when a microplate Protocol "parks" at the last well imaged instead of the next to be imaged well between intervals. In this case the initiation of the XY translation will be the t=0 rather than the first image acquisition. So the first well of a multi-well Protocol is slightly different in timing, not important if the interval is 8 hours but at 6 seconds it's very noticeable.
The text was updated successfully, but these errors were encountered: