From ce5545f8a3d0c72b8ded1fbf00b9cfb1fbfaddd7 Mon Sep 17 00:00:00 2001 From: Keoni Gandall Date: Tue, 15 Oct 2024 15:42:15 -0700 Subject: [PATCH] parts --- parts/linkers.md | 15 +- parts/parts/bsub.yaml | 6 - parts/parts/ecoli/ecoli.yaml | 103 ----------- parts/parts/ecoli/origins.yaml | 52 ------ parts/parts/ecoli/promoters.yaml | 101 ----------- parts/parts/vnat.yaml | 290 ++++++++++++++++++++++++------- 6 files changed, 232 insertions(+), 335 deletions(-) delete mode 100644 parts/parts/ecoli/ecoli.yaml delete mode 100644 parts/parts/ecoli/origins.yaml delete mode 100644 parts/parts/ecoli/promoters.yaml diff --git a/parts/linkers.md b/parts/linkers.md index 4cb98b4..1bb5f8b 100644 --- a/parts/linkers.md +++ b/parts/linkers.md @@ -109,8 +109,8 @@ Often, users will want to move transcription units to new organisms of interest. * [C] AATG - ATCC (cds) * [T] ATCC - CGCT (terminator) * [B] CGCT - GTCT (linker suffix) -* [D] GTCT - AAGC (target origin of replication) -* [S] AAGC - ATAG (target selective marker) +* [S] GTCT - AAGC (target selective marker) +* [D] AAGC - ATAG (target origin of replication) * [E2] ATAG - CGAG (e coli vector 2) ``` @@ -125,10 +125,10 @@ Instead of shuttle vectors, users will sometimes want to integrate sections of D * [P] TACA - AACT (promoter) * [R] AACT - AATG (rbs) * [C] AATG - ATCC (cds) -* [T] ATCC - GTCT (terminator) -* [B] GTCT - ATAG (linker suffix) -* [D] GTCT - AAGC (target ori / downstream homology) -* [S] AAGC - ATAG (target selective marker) +* [T] ATCC - CGCT (terminator) +* [B] CGCT - GTCT (linker suffix) +* [S] GTCT - AAGC (target selective marker) +* [D] AAGC - ATAG (target upstream homology) * [EC3] ATAG - ATTA (e coli vector 3) * [U] ATTA - CGAG (upstream homology) ``` @@ -240,5 +240,4 @@ This collection has 384 linkers. There are 96 linkers for building independent t Each overhang is assigned a number. The reverse complement of each overhang is represented as the negative version of its number, which is also how we are able to flip constructs. -Linkers are named with a 1 letter + 3 number scheme, separated by underscores ( _ ) in the format `Y_X_X_X`. Y describes the direction (F, or forward, for prefix, and R, or reverse, for suffix) of the linker. The first 2 numbers describe the two overhangs which the linker itself will be cut out with, the third number describes the overhang which the linker will introduce to the construct. After a GoldenGate assembly and transformation, anything between the prefix and suffix lin -kers can be cut out with BsaI and used in another assembly. +Linkers are named with a 1 letter + 3 number scheme, separated by underscores ( _ ) in the format `Y_X_X_X`. Y describes the direction (F, or forward, for prefix, and R, or reverse, for suffix) of the linker. The first 2 numbers describe the two overhangs which the linker itself will be cut out with, the third number describes the overhang which the linker will introduce to the construct. After a GoldenGate assembly and transformation, anything between the prefix and suffix linkers can be cut out with BsaI and used in another assembly. diff --git a/parts/parts/bsub.yaml b/parts/parts/bsub.yaml index 6b3bdfa..291dc0d 100644 --- a/parts/parts/bsub.yaml +++ b/parts/parts/bsub.yaml @@ -100,12 +100,6 @@ PxylA_xylR: suffix: AACT tags: [promoter] sequence: AGCGGATAACAATTTCACACAGGAGGCCGATCGCGTTATGCAGCAATGGCAAGAACGTCCCGGGGAGCTCCTAACTTATAGGGGTAACACTTAAAAAAGAATCAATAACGATAGAAACCGCTCCTAAAGCAGGTGCATTTTTTCCTAACGAAGAAGGCAATAGTTCACATTTATTGTCTAAATGAGAATGGACTCTAGAAGAAACTTCGTTTTTAATCGTATTTAAAACAATGGGATGAGATTCAATTATATGATTTCTCAAGATAACAGCTTCTATATCAAATGTATTAAGGATATTGGTTAATCCAATTCCGATATAAAAGCCAAAGTTTTGAAGTGCATTTAACATTTCTACATCATTTTTATTTGCGCGTTCCACAATCTCTTTTCGAGAAATATTCTTTTCTTCTTTAGAGAGCGAAGCCAGTAACGCTTTTTCAGAAGCATATAATTCCCAACAGCCTCGATTTCCACAGCTGCATTTGGGTCCATTAAAATCTATCGTCATATGACCCATTTCCCCAGAAAAACCCTGAACACCTTTATACAATTCGTTGTTAATAACAAGTCCAGTTCCAATTCCGATATTAATACTGATGTAAACGATGTTTTCATAGTTTTTTGTCATACCAAATACTTTTTCACCGTATGCTCCTGCATTAGCTTCATTTTCAACAAAAACCGGAACATTAAACTCACTCTCAATTAAAAACTGCAAATCTTTGATATTCCAATTTAAGTTAGGCATGAAAATAATTTGCTGATGACGATCTACAAGGCCTGGAACACAAATTCCTATTCCGACTAGACCATAAGGGGACTCAGGCATATGGGTTACAAAACCATGAATAAGTGCAAATAAAATCTCTTTTACTTCACTAGCGGAAGAACTAGACAAGTCAGAAGTCTTCTCGAGAATAATATTTCCTTCTAAGTCGGTTAGAATTCCGTTAAGATAGTCGACTCCTATATCAATACCAATCGAGTAGCCTGCATTCTTATTAAAAACAAGCATTACAGGTCTTCTGCCGCCTCTAGATTGCCCTGCCCCAATTTCAAAAATAAAATCTTTTTCAAGCAGTGTATTTACTTGAGAGGAGACAGTAGACTTGTTTAATCCTGTAATCTCAGAGAGAGTTGCCCTGGAGACAGGGGAGTTCTTCAAAATTTCATCTAATATTAATTTTTGATTCATTTTTTTTACTAAAGCTTGATCTGCAATTTGAATAATAACCACTCCTTTGTTTATCCACCGAACTAAGTTGGTGTTTTTTGAAGCTTGAATTAGATATTTAAAAGTATCATATCTAATATTATAACTAAATTTTCTAAAAAAAACATTGAAATAAACATTTATTTTGTATATGATGAGATAAAGTTAGTTTATTGGATAAACAAACTAACTCAATTAAGATAGTTGATGGATAAACTTGTTCACTTAAATCAAAGGCTGAG -mRuby_with_RBS(Bsub): - description: mRuby with ribosomal binding site for Bacillus - prefix: AACT - suffix: ATCC - tags: [rbs_cds, reporter] - sequence: US_glmS: description: upstream glmS flank prefix: ATTA diff --git a/parts/parts/ecoli/ecoli.yaml b/parts/parts/ecoli/ecoli.yaml deleted file mode 100644 index a31b528..0000000 --- a/parts/parts/ecoli/ecoli.yaml +++ /dev/null @@ -1,103 +0,0 @@ -# CIDAR -eBFP2: - description: EBFP2 is a basic (constitutively fluorescent) blue fluorescent protein published in 2007, derived from Aequorea victoria. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity. - prefix: - suffix: - references: ["https://www.fpbase.org/protein/ebfp2/"] - sequence: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgaggggcgagggcgagggcgatgccaccaacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgagccacggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcacctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcgtcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacttcaacagccacaacatctatatcatggccgtcaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacgtggaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacagccactacctgagcacccagtccgtgctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttccgcaccgccgccgggatcactctcggcatggacgagctgtacaagtaa -E1010m: - description: Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. - sequence: atggcttcctccgaggatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaggatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaggactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa -E0040m: - description: Fluorescent protein. Green. Modified from Bba_E0040 to fix illegal site. - sequence: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa -E0030: - description: Fluorescent protein. Yellow. - sequence: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa -cre: - description: cre/lox - sequence: atgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcgaatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattag -araC: - description: Controller protein, araC repressor/activator (activates pBAD, I13453). Otherwise known as C0080 - sequence: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataa -luxR: - description: Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062). Annotated as C0062. - sequence: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataa -tetR: - description: Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) - sequence: ATGtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataa -lacI: - description: Controller protein, lacI repressor (in concert with CAP, represses pLacI, R0010) - sequence: ATGatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaggacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagatagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtag -BCD12: - description: RBS - BiCistronic Design, medium strength (RBS part type, actually contains a small transcriptional unit and second RBS) - sequence: gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctgcggagggtttct -B0032m: - description: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system - sequence: agagtcacacaggaaagtacta -BCD8: - description: RBS - BiCistronic Design, low strength (RBS part type, actually contains a small transcriptional unit and second RBS) - sequence: gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcatcggaccgtttct -BCD2: - description: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) - sequence: gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttct -B0034m: - description: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. - sequence: agagaaagaggagaaatacta -B0033m: - description: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. - sequence: agagtcacacaggactacta -B0015: - description: Double terminator (B0010,B0012) - sequence: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata -pBAD: - description: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) - seqeunce: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc - -# -# Ecoflex -# - -eCFP(Ecoli): - description: placeholder - prefix: A - suffix: GGATCC - sequence: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgcc -eGFP(Ecoli): - description: placeholder - prefix: A - suffix: GGATCC - sequence: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa -mCherry(Ecoli): - description: placeholder - prefix: A - suffix: GGATCC - sequence: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag - -# -# CIDAR extension -# -J23101: - description: MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - sequence: TTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGC -J23150: - description: MoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - sequence: TGCTGTTCCGCTGGGCATGCTTTACGGCTAGCTCAGTCCTAGGTATTATGC -J23151: - description: MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - sequence: TGCTGTTCCGCTGGGCATGCTTGATGGCTAGCTCAGTCCTAGGTACAATGC -pOR1-OR2: - description: MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - sequence: TGCTGTTCCGCTGGGCATGCTGAGCTAACACCGTGCGTGTTGACAATTTTACCTCTGGCGGTGATAATGGTTGCAGC -Ptet: - description: MoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). - sequence: CTAGCAAGGGTCCGGGTTCACCGAAATCTATCTCATTTGCTAGTTATAAAATTATGAAATTTGCGTAAATTCCCTATCAGTGATAGAGATTCAGAAGCAAT -pCin-TetO: - description: MoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). - sequence: CGGAACTCTTGTGCGTGGCGCATGTCGTTATCGCAAAACCGCTGCACACTTTTGCGCGACATGCTCTGATCCCCCTCATCTGGGGGGGCCTATCTGAGGGAATTTACTTCCCTATCAGTGATAGAGATAATGAGCACATCCCTATCAGTGATAGAGATG -pCin-LacO: - description: MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). - sequence: CGGAACTCTTGTGCGTGGCGCATGTCGTTATCGCAAAACCGCTGCACACTTTTGCGCGACATGCTCTGATCCCCCTCATCTGGGGGGGCCTATCTGAGGGAATTTACTTGTGAGCGCTCACAATTGATAATGAGCACAATTGTGAGCGGATAACAATTGCT -T7_promoter: - description: The T7 promoter binding site - sequence: TAATACGACTCACTATAG diff --git a/parts/parts/ecoli/origins.yaml b/parts/parts/ecoli/origins.yaml deleted file mode 100644 index 17a3808..0000000 --- a/parts/parts/ecoli/origins.yaml +++ /dev/null @@ -1,52 +0,0 @@ -# Origins -ColE1: - description: High copy (500-700 copies) common Escherichia coli origin - sequence: TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA -RSF1030: - description: High copy (>100 copies) Escherichia coli origin. Compatible with pUC/pBR322, p15A, ColA, CloDF13, and SC101 - sequence: AACGGAATAGCTGTTCGTTGACTTGATAGACCGATTGATTCATCATCTCATAAATAAAGAAAAACCACCGCTACCAACGGTGGTTTTCTCAAGGTTCGCTGAGCTACCAACTCTTTGAACCAAGGTAAGTGGGTTGGAGGACCGCACTCACCAAAATCTGTTCTTTCAGTTTAGCCTTAACAGGTGCATAACTTCAAGACAAAGTCCTCTAAATCAGTTACCAATGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCAACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGGCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCTGCGGCGTTGGCTTCTTCCGGTGCTTTGCTTTTTGCTCACATGTTCTTTCCGGCTTTATCCCCTGATTCTGTGGATAACCGTATTACCGCTTTTGAGTGAGCTGACACCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAGA -pBR322: - description: Medium copy (~20) common Escherichia coli origin - sequence: CCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGTGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGCAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGG -p15A: - description: Medium copy (10-12 copies) common Escherichia coli origin. pUC/pBR322, SC101, ColA, CloDF13, and RSF1030 - sequence: TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA -ColE2: - description: Medium copy plasmid. Can be engineered easily for different copy number levels. Orthogonal to R6K origin - sequence: TTAAGGGGGGCGTACAACGGGAGTTATGGTAAATGGATCGGGTTTTCGGGAAGGATCGACAGGATTTGCCGTTGGGTGTAGTGTAAGCGACTGAAAAACAAACGCCCCGTAAATCGTGCTCTCACCGCCAAGATTGATCACGAAATTACAGGGCGCCGGGTTCCGCGTTTCCCGATGGGAAAGCGCGGTTAGTTAAACTGTGTACCGAGAGAAATCGTATCACATGAGCGCCGTACTTCAACGCTTCAGGGAAAAATTACCGCACAAACCGTACTGTACGAACGATTTCGCGTACGGCGTTCGCATTCTGCCGAAAAACATTGCCATTCTTGCCCGTTTCATCCAGCAGAACCAGCCACATGCACTGTACTGGCTTCCCTTTGACGTGGACCGGACGGGGGCATCAATCGACTGGAGCGACCGGAATTGTCCGGCCCCGAACATCACCGTAAAAAATCCCCGTAACGGGCACGCGCATCTGCTCTACGCGCTCGCCCTTCCTGTGAGAACTGCGCCGGATGCATCGGCTTCGGCGCTCAGATACGCTGCCGCTATTGAGCGTGCGTTGTGTGAAAAACTGGGCGCGGATGTGAATTACAGCGGCCTGATCTGCAAAAATCCGTGCCACCCTGAATGGCAGGAAGTGGAATGGCGCGAGGAACCCTACACTCTCGACGAACTGGCTGATTATCTCGATTTGAGCGCCTCAGCGCGCCGTAGCGTCGATAAAAATTACGGGCTGGGGCGAAACTACCATCTGTTCGAAAAGGTCCGTAAATGGGCCTACAGAGCGATTCGTCAGGGCTGGCCTGTATTCTCACAATGGCTTGATGCCGTGATCCAGCGTGTCGAAATGTACAACGCATCGCTTCCCGTTCCGCTTTCTCCGGCTGAATGTCGGGCTATTGGCAAGAGCATTGCGAAATATACACACAGGAAATTCTCACCAGAGGGATTTTCCGCTGTACAGGCCGCTCGAGGTCGCAAGGGCGGAACTAAATCTAAGCGCGCAGCAGTTCCTACATCAGCACGTTCGCTGAAGCCGTGGGAGGCATTAGGGATCAGTCGAGCGACGTACTACCGAAAATTAAAATGTGATCCAGATCTCGCAAAATAAGACCAGATAAGCCTTATCAGATAACAGCGCCCTTTTGGCGTCTTTTTGAGCAGTGATCTTTCTTGCGATTTTCTCCGTTCTGC -pWV01: - description: Medium copy broad host range plasmid. - sequence: CGATTTTTTATTAAAATTTTAGCGTTTATTTCGTTTAGTTATCGGCATAATCGTTAAAACAGGCGTTATCGTAGCGTAAAAGCCCTTGAGCGTAGCGTGGCTTTGCAGCGAAGATGTTGTCTGTTAGATTATGAAAGCCGATGACTGAATGAAATAATAAGCGCAGCGCCCTTCTATTTCGGTTGGAGGAGGCTCAAGGGAGTATGAGGGAATGAAATTCCCTCATGGGTTTGATTTTAAAAATTGCTTGCAATTTTGCCGAGCGGTAGCGCTGGAAAATTTTTGAAAAAAATTTGGAATTTGGAAAAAAATGGGGGGAAAGGAAGCGAATTTTGCTTCCGTACTACGACCCCCCATTAAGTGCCGAGTGCCAATTTTTGTGCCAAAAACGCTCTATCCCAACTGGCTCAAGGGTTTAAGGGGTTTTTCAATCGCCAACGAATCGCCAACGTTTTCGCCAACGTTTTTTATAAATCTATATTTAAGTAGCTTTATTGTTGTTTTTATGATTACAAAGTGATACACTAACTTTATAAAATTATTTGATTGGAGTTTTTTAAATGGTGATTTCAGAATCGAAAAAAAGAGTTATGATTTCTCTGACAAAAGAGCAAGATAAAAAATTAACAGATATGGCGAAACAAAAAGGTTTTTCAAAATCTGCGGTTGCGGCGTTAGCTATAGAAGAATATGCAAGAAAGGAATCAGAACAAAAAAAATAAGCGAAAGCTCGCGTTTTTAGAAGGATACGAGTTTTCGCTACTTGTTTTTGATAAGGTAATTATATCATGGCTATTAAAAATACTAAAGCTAGAAATTTTGGATTTTTATTATATCCTGACTCAATTCCTAATGATTGGAAAGAAAAATTAGAGAGTTTGGGCGTATCTATGGCTGTCAGTCCTTTACACGATATGGACGAAAAAAAAGATAAAGATACATGGAATAGTAGTGATGTTATACGAAATGGAAAGCACTATAAAAAACCACACTATCACGTTATATATATTGCACGAAATCCTGTAACAATAGAAAGCGTTAGGAACAAGATTAAGCGAAAATTGGGGAATAGTTCAGTTGCTCATGTTGAGATACTTGATTATATCAAAGGTTCATATGAATATTTGACTCATGAATCAAAGGACGCTATTGCTAAGAATAAACATATATACGACAAAAAAGATATTTTGAACATTAATGATTTTGATATTGACCGCTATATAACACTTGATGAAAGCCAAAAAAGAGAATTGAAGAATTTACTTTTAGATATAGTGGATGACTATAATTTGGTAAATACAAAAGATTTAATGGCTTTTATTCGCCTTAGGGGAGCGGAGTTTGGAATTTTAAATACGAATGATGTAAAAGATATTGTTTCAACAAACTCTAGCGCCTTTAGATTATGGTTTGAGGGCAATTATCAGTGTGGATATAGAGCAAGTTATGCAAAGGTTCTTGATGCTGAAACGGGGGAAATAAAATGACAAACAAAGAAAAAGAGTTATTTGCTGAAAATGAGGAATTAAAAAAAGAAATTAAGGACTTAAAAGAGCGTATTGAAAGATACAGAGAAATGGAAGTTGAATTAAGTACAACAATAGATTTATTGAGAGGAGGGATTATTGAATAAATAAAAGCCCCCTGACGAAAGTCGAAGGGGGTTTTTATTTTGGTTTGATGTTGCGATTAATAGCAATACAATTGCAATAAACAAAATGATC -pBAV1KT5: - description: High copy (~350 E.coli, ~70 Bacillus subtilis, ~3 Agrobacterium tumefaciens, ~60 Acinetobacter baylyi, 100-500 Streptococcus pneumoniae) broad host range vector derived from pWV01. - description: CCAAGCACTAGGCGATTTTTTATTAAAATTTTAGCGTTTATTTCGTTTAGTTATCGGCATAATCGTTAAAACAGGCGTTATCGTAGCGTAAAAGCCCTTGAGCGTAGCGTGGCTTTGCAGCGAAGATGTTGTCTGTTAGATTATGAAAGCCGATGACTGAATGAAATAATAAGCGCAGCGCCCTTCTATTTCGGTTGGAGGAGGCTCAAGGGAGTATGAGGGAATGAAATTCCCTCATGGGTTTGATTTTAAAAATTGCTTGCAATTTTGCCGAGCGGTAGCGCTGGAAAATTTTTGAAAAAAATCTGGAATTTGGAAAAAAATGGGGGGAAAGGAAGCGAATTTTGCTTCCGTACTACGACCCCCCATTAAGTGCCGAGTGCCAATTTTTGTGCCAAAAACGCTCTATCCCAACTGGCTCAAGGGTTTAAGGGGTTTTTCAATCGCCAACGAATCGCCAACGTTTTCGCCAACGTTTTTTATAAATCTATATTTAAGTAGCTTTATTGTTGTTTTTATGATTACAAAGTGATACACTAACTTTATAAAATTATTTGATTGGAGTTTTTTAAATGGTGATTTCAGAATCGAAAAAAAGAGTTATGATTTCTCTGACAAAAGAGCAAGATAAAAAATTAACAGATATGGCGAAACAAAAAGGTTTTTCAAAATCTGCGGTTGCGGCGTTAGCTATAGAAGAATATGCAAGAAAGGAATCAGAACAAAAAAAATAAGCGAAAGCTCGCGTTTTTAGAAGGATACGAGTTTTCGCTACTTGTTTTTGATAAGGTAATTATATCATGGCTATTAAAAATACTAAAGCTAGAAATTTTGGATTTTTATTATATCCTGACTCAATTCCTAATGATTGGAAGGAAAAATTAGAGAGTTTGGGCGTATCTATGGCTGTCAGTCCTTTACACGATATGGACGAAAAAAAAGATAAAGATACATGGAATAGTAGTGATGTTATACGAAATGGAAAGCACTATAAAAAACCACACTATCACGTTATATATATTGCACGAAATCCTGTAACAATAGAAAGCGTTAGGAACAAGATTAAGCGAAAATTGGGGAATAGTTCAGTTGCTCATGTTGAGATACTTGATTATATCAAAGGTTCATATGAATATTTGACTCATGAATCAAAGGACGCTATTGCTAAGAATAAACATATATACGACAAAAAAGATATTTTGAACATTAATGATTTTGATATTGACCGCTATATAACACTTGATGAAAGCCAAAAAAGAGAATTGAAGAATTTACTCTTAGATATAGTGGATGACTATAATTTGGTAAATACAAAAGATTTAATGGCTTTTATTCGCCTTAGGGGAGCGGAGTTTGGAATTTTAAATACGAATGATGTAAAAGATATTGTTTCAACAAACTCTAGCGCCTTTAGATTATGGTTTGAGGGCAATTATCAGTGTGGATATAGAGCAAGTTATGCAAAGGTTCTTGATGCTGAAACGGGGGAAATAAAATGACACTAGGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTG -RSF1010: - description: Low-med copy very broad host range origin (most gram-negative and some gram-positive) - sequence: GTCCTGGCCGCCACATGAGGCATGTTCCGCGTCCTTGCAATACTGTGTTTACATACAGTCTATCGCTTAGCGGAAAGTTCTTTTACCCTCAGCCGAAATGCCTGCCGTTGCTAGACATTGCCAGCCAGTGCCCGTCACTCCCGTACTAACTGTCACGAACCCCTGCAATAACTGTCACGCCCCCCTGCAATAACTGTCACGAACCCCTGCAATAACTGTCACGCCCCCAAACCTGCAAACCCAGCAGGGGCGGGGGCTGGCGGGGTGTTGGAAAAATCCATCCATGATTATCTAAGAATAATCCACTAGGCGCGGTTATCAGCGCCCTTGTGGGGCGCTGCTGCCCTTGCCCAATATGCCCGGCCAGAGGCCGGATAGCTGGTCTATTCGCTGCGCTAGGCTACACACCGCCCCACCGCTGCGCGGCAGGGGGAAAGGCGGGCAAAGCCCGCTAAACCCCACACCAAACCCCGCAGAAATACGCTGGAGCGCTTTTAGCCGCTTTAGCGGCCTTTCCCCCTACCCGAAGGGTGGGGGCGCGTGTGCAGCCCCGCAGGGCCTGTCTCGGTCGATCATTCAGCCCGGCTCATCCTTCTGGCGTGGCGGCAGACCGAACAAGGCGCGGTCGTGGTCGCGTTCAAGGTACGCATCCATTGCCGCCATGAGCCGATCCTCCGGCCACTCGCTGCTGTTCACCTTGGCCAAAATCATGGCCCCCACCAGCACCTTGCGCCTTGTTTCGTTCTTGCGCTCTTGCTGCTGTTCCCTTGCCCGCACCCGCTGAATTTCGGCATTGATTCGCGCTCGTTGTTCTTCGAGCTTGGCCAGCCGATCCGCCGCCTTGTTGCTCCCCTTAACCATCTTGACACCCCATTGTTAATGTGCTGTCTCGTAGGCTATCATGGAGGCACAGCGGCGGCAATCCCGACCCTACTTTGTAGGGGAGGGCGCACTTACCGGTTTCTCTTCGAGAAACTGGCCTAACGGCCACCCTTCGGGCGGTGCGCTCTCCGAGGGCCATTGCATGGAGCCGAAAAGCAAAAGCAACAGCGAGGCAGCATGGCGATTTATCACCTTACGGCGAAAACCGGCAGCAGGTCGGGCGGCCAATCGGCCAGGGCCAAGGCCGACTACATCCAGCGCGAAGGCAAGTATGCCCGCGACATGGATGAAGTCTTGCACGCCGAATCCGGGCACATGCCGGAGTTCGTCGAGCGGCCCGCCGACTACTGGGATGCTGCCGACCTGTATGAACGCGCCAATGGGCGGCTGTTCAAGGAGGTCGAATTTGCCCTGCCGGTCGAGCTGACCCTCGACCAGCAGAAGGCGCTGGCGTCCGAGTTCGCCCAGCACCTGACCGGTGCCGAGCGCCTGCCGTATACGCTGGCCATCCATGCCGGTGGCGGCGAGAACCCGCACTGCCACCTGATGATCTCCGAGCGGATCAATGACGGCATCGAGCGGCCCGCCGCTCAGTGGTTCAAGCGGTACAACGGCAAGACCCCGGAGAAGGGCGGGGCACAAAAGACCGAAGCGCTCAAGCCCAAGGCATGGCTTGAGCAGACCCGCGAGGCATGGGCCGACCATGCCAACCGGGCATTAGAGCGGGCTGGCCACGACGCCCGCATTGACCACAGAACACTTGAGGCGCAGGGCATCGAGCGCCTGCCCGGTGTTCACCTGGGGCCGAACGTGGTGGAGATGGAAGGCCGGGGCATCCGCACCGACCGGGCAGACGTGGCCCTGAACATCGACACCGCCAACGCCCAGATCATCGACTTACAGGAATACCGGGAGGCAATAGACCATGAACGCAATCGACAGAGTGAAGAAATCCAGAGGCATCAACGAGTTAGCGGAGCAGATCGAACCGCTGGCCCAGAGCATGGCGACACTGGCCGACGAAGCCCGGCAGGTCATGAGCCAGACCCAGCAGGCCAGCGAGGCGCAGGCGGCGGAGTGGCTGAAAGCCCAGCGCCAGACAGGGGCGGCATGGGTGGAGCTGGCCAAAGAGTTGCGGGAGGTAGCCGCCGAGGTGAGCAGCGCCGCGCAGAGCGCCCGGAGCGCGTCGCGGGGGTGGCACTGGAAGCTATGGCTAACCGTGATGCTGGCTTCCATGATGCCTACGGTGGTGCTGCTGATCGCATCGTTGCTCTTGCTCGACCTGACGCCACTGACAACCGAGGACGGCTCGATCTGGCTGCGCTTGGTGGCCCGATGAAGAACGACAGGACTTTGCAGGCCATAGGCCGACAGCTCAAGGCCATGGGCTGTGAGCGCTTCGATATCGGCGTCAGGGACGCCACCACCGGCCAGATGATGAACCGGGAATGGTCAGCCGCCGAAGTGCTCCAGAACACGCCATGGCTCAAGCGGATGAATGCCCAGGGCAATGACGTGTATATCAGGCCCGCCGAGCAGGAGCGGCATGGTCTGGTGCTGGTGGACGACCTCAGCGAGTTTGACCTGGATGACATGAAAGCCGAGGGCCGGGAGCCTGCCCTGGTAGTGGAAACCAGCCCGAAGAACTATCAGGCATGGGTCAAGGTGGCCGACGCCGCAGGCGGTGAACTTCGGGGGCAGATTGCCCGGACGCTGGCCAGCGAGTACGACGCCGACCCGGCCAGCGCCGACAGCCGCCACTATGGCCGCTTGGCGGGCTTCACCAACCGCAAGGACAAGCACACCACCCGCGCCGGTTATCAGCCGTGGGTGCTGCTGCGTGAATCCAAGGGCAAGACCGCCACCGCTGGCCCGGCGCTGGTGCAGCAGGCTGGCCAGCAGATCGAGCAGGCCCAGCGGCAGCAGGAGAAGGCCCGCAGGCTGGCCAGCCTCGAACTGCCCGAGCGGCAGCTTAGCCGCCACCGGCGCACGGCGCTGGACGAGTACCGCAGCGAGATGGCCGGGCTGGTCAAGCGCTTCGGTGATGACCTCAGCAAGTGCGACTTTATCGCCGCGCAGAAGCTGGCCAGCCGGGGCCGCAGTGCCGAGGAAATCGGCAAGGCCATGGCCGAGGCCAGCCCAGCGCTGGCAGAGCGCAAGCCCGGCCACGAAGCGGATTACATCGAGCGCACCGTCAGCAAGGTCATGGGTCTGCCCAGCGTCCAGCTTGCGCGGGCCGAGCTGGCACGGGCACCGGCACCCCGCCAGCGAGGCATGGACAGGGGCGGGCCAGATTTCAGCATGTAGTGCTTGCGTTGGTACTCACGCCTGTTATACTATGAGTACTCACGCACAGAAGGGGGTTTTATGGAATACGAAAAAAGCGCTTCAGGGTCGGTCTACCTGATCAAAAGTGACAAGGGCTATTGGTTGCCCGGTGGCTTTGGTTATACGTCAAACAAGGCCGAGGCTGGCCGCTTTTCAGTCGCTGATATGGCCAGCCTTAACCTTGACGGCTGCACCTTGTCCTTGTTCCGCGAGGACAAGCCTTTCGGCCCCGGCAAGTTTCTCGGTGACTGATATGAAAGACCAAAAGGACAAGCAGACCGGCGACCTGCTGGCCAGCCCTGACGCTGTACGCCAAGCGCGATATGCCGAGCGCATGAAGGCCAAAGGGATGCGTCAGCGCAAGTTCTGGCTGACCGACGACGAATACGAGGCGCTGCGCGAGTGCCTGGAAGAACTCAGAGCGGCGCAGGGCGGGGGTAGTGACCCCGCCAGCGCCTAACCACCAACTGCCTGCAAAGGAGGCAATCAATGGCTACCCATAAGCCTATCAATATTCTGGAGGCGTTCGCAGCAGCGCCGCCACCGCTGGACTACGTTTTGCCCAACATGGTGGCCGGTACGGTCGGGGCGCTGGTGTCGCCCGGTGGTGCCGGTAAATCCATGCTGGCCCTGCAACTGGCCGCACAGATTGCAGGCGGGCCGGATCTGCTGGAGGTGGGCGAACTGCCCACCGGCCCGGTGATCTACCTGCCCGCCGAGGACCCGCCCACCGCCATTCATCACCGCCTGCACGCCCTTGGGGCGCACCTCAGCGCCGAGGAACGGCAAGCCGTGGCTGACGGCCTGCTGATCCAGCCGCTGATCGGCAGCCTGCCCAACATCATGGCCCCGGAGTGGTTCGACGGCCTCAAGCGCGCCGCCGAGGGCCGCCGCCTGATGGTGCTGGACACGCTGCGCCGGTTCCACATCGAGGAAGAAAACGCCAGCGGCCCCATGGCCCAGGTCATCGGTCGCATGGAGGCGATCGCCGCCGATACCGGGTGCTCTATCGTGTTCCTGCACCATGCCAGCAAGGGCGCGGCCATGATGGGCGCAGGCGACCAGCAGCAGGCCAGCCGGGGCAGCTCGGTACTGGTCGATAACATCCGCTGGCAGTCCTACCTGTCGAGCATGACCAGCGCCGAGGCCGAGGAATGGGGTGTGGACGACGACCAGCGCCGGTTCTTCGTCCGCTTCGGTGTGAGCAAGGCCAACTATGGCGCACCGTTCGCTGATCGGTGGTTCAGGCGGCATGACGGCGGGGTGCTCAAGCCCGCCGTGCTGGAGAGGCAGCGCAAGAGCAAGGGGGTGCCCCGTGGTGAAGCCTAAGAACAAGCACAGCCTCAGCCACGTCCGGCACGACCCGGCGCACTGTCTGGCCCCCGGCCTGTTCCGTGCCCTCAAGCGGGGCGAGCGCAAGCGCAGCAAGCTGGACGTGACGTATGACTACGGCGACGGCAAGCGGATCGAGTTCAGCGGCCCGGAGCCGCTGGGCGCTGATGATCTGCGCATCCTGCAAGGGCTGGTGGCCATGGCTGGGCCTAATGGCCTAGTGCTTGGCCCGGAACCCAAGACCGAAGGCGGACGGCAGCTCCGGCTGTTCCTGGAACCCAAGTGGGAGGCCGTCACCGCTGAATGCCATGTGGTCAAAGGTAGCTATCGGGCGCTGGCAAAGGAAATCGGGGCAGAGGTCGATAGTGGTGGGGCGCTCAAGCACATACAGGACTGCATCGAGCGCCTTTGGAAGGTATCCATTATCGCCCAGAATGGCCGCAAGCGGCAGGGGTTTCGGCTGCTGTCGGAGTACGCCAGCGACGAGGCGGACGGGCGCCTGTACGTGGCCCTGAACCCCTTGATCGCGCAGGCCGTCATGGGTGGCGGCCAGCATGTGCGCATCAGCATGGACGAGGTGCGGGCGCTGGACAGCGAAACCGCCCGCCTGCTGCACCAGCGGCTGTGTGGCTGGATCGACCCCGGCAAAACCGGCAAGGCTTCCATAGATACCTTGTGCGGCTATGTCTGGCCGTCAGAGGCCAGTGGTTCGACCATGCGCAAGCGCCGCCAGCGGGTGCGCGAGGCGTTGCCGGAGCTGGTCGCGCTGGGCTGGACGGTAACCGAGTTCGCGGCGGGCAAGTACGACATCACCCGGCCCAAGGCGGCAGGCTGACCCCCCCCACTCTATTGTAAACAAGACATTTTTATCTTTTATATTCAATGGCTTATTTTCCTGCTAATTGGTAATACCATGAAAAATACCATGCTCAGAAAAGGCTTAACAATATTTTGAAAAATTGCCTACTGAGCGCTGCCGCACAGCTCCATAGGCCGCTTTCCTGGCTTTGCTTCCAGATGTATGCTCTTATGCTCC -SC101: - description: Low copy (~5 copies) Escherichia coli origin. Compatible with pUC/pBR322, p15A, ColA, CloDF13, and RSF1030 - sequence: ATGCAGCGATTTGCCCGAGCTTGCGAGGGTGCTACTTAAGCCTTTAGGGTTTTAAGGTCTGTTTTGTAGAGGAGCAAACAGCGTTTGCGACATCCTTTTGTAATACTGCGGAACTGACTAAAGTAGTGAGTTATACACAGGGCTGGGATCTATTCTTTTTATCTTTTTTTATTCTTTCTTTATTCTATAAATTATAACCACTTGAATATAAACAAAAAAAACACACAAAGGTCTAGCGGAATTTACAGAGGGTCTAGCAGAATTTACAAGTTTTCCAGCAAAGGTCTAGCAGAATTTACAGATACCCACAACTCAAAGGAAAAGGACTAGTAATTATCATTGACTAGCCCATCTCAATTGGTATAGTGATTAAAATCACCTAGACCAATTGAGATGTATGTCTGAATTAGTTGTTTTCAAAGCAAATGAACTAGCGACTAGTCGCTATGACTTAACGGAGCATGAAACCAAGCTAATTTTATGCTGTGTGGCACTACTCAACCCCACGATTGAAAACCCTACAAGGAAAGAACGGACGGTATCGTTCACTTATAACCAATACGTTCAGATGATGAACATCAGTAGGGAAAATGCTTATGGTGTATTAGCTAAAGCAACCAGAGAGCTGATGACGAGAACTGTGGAAATCAGGAATCCTTTGGTTAAAGGCTTTGAGATTTTCCAGTGGACAAACTATGCCAAGTTCTCAAGCGAAAAATTAGAATTAGTTTTTAGTGAAGAGATATTGCCTTATCTTTTCCAGTTAAAAAAATTCATAAAATATAATCTGGAACATGTTAAGTCTTTTGAAAACAAATACTCTATGAGGATTTATGAGTGGTTATTAAAAGAACTAACACAAAAGAAAACTCACAAGGCAAATATAGAGATTAGCCTTGATGAATTTAAGTTCATGTTAATGCTTGAAAATAACTACCATGAGTTTAAAAGGCTTAACCAATGGGTTTTGAAACCAATAAGTAAAGATTTAAACACTTACAGCAATATGAAATTGGTGGTTGATAAGCGAGGCCGCCCGACTGATACGTTGATTTTCCAAGTTGAACTAGATAGACAAATGGATCTCGTAACCGAACTTGAGAACAACCAGATAAAAATGAATGGTGACAAAATACCAACAACCATTACATCAGATTCCTACCTACATAACGGACTAAGAAAAACACTACACGATGCTTTAACTGCAAAAATTCAGCTCACCAGTTTTGAGGCAAAATTTTTGAGTGACATGCAAAGTAAGTATGATCTCAATGGTTCGTTCTCATGGCTCACGCAAAAACAACGAACCACACTAGAGAACATACTGGCTAAATACGGAAGGATCTGAGGTTCTTATGGCTCTTGTATCTATCAGTGAAGCATCAAGACTAACAAACAAAAGTAGAACAACTGTTCACCGTTACATATCAAAGGGAAAACTGTCCATATGCACAGATGAAAACGGTGTAAAAAAGATAGATACATCAGAGCTTTTACGAGTTTTTGGTGCA -pRK2: - description: Low copy (4-7 copies) origin with broad gram-negative host range - sequence: GTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGGGGGATCAGGACCGCTGCCGGAGCGCAACCCACTCACTACAGCAGAGCCATGTAGGGCCGCCGGCGTTGTGGATACCTCGCGGAAAACTTGGCCCTCACTGACAGATGAGGGGCGGACGTTGACACTTGAGGGGCCGACTCACCCGGCGCGGCGTTGACAGATGAGGGGCAGGCTCGATTTCGGCCGGCGACGTGGAGCTGGCCAGCCTCGCAAATCGGCGAAAACGCCTGATTTTACGCGAGTTTCCCACAGATGATGTGGACAAGCCTGGGGATAAGTGCCCTGCGGTATTGACACTTGAGGGGCGCGACTACTGACAGATGAGGGGCGCGATCCTTGACACTTGAGGGGCAGAGTGCTGACAGATGAGGGGCGCACCTATTGACATTTGAGGGGCTGTCCACAGGCAGAAAATCCAGCATTTGCAAGGGTTTCCGCCCGTTTTTCGGCCACCGCTAACCTGTCTTTTAACCTGCTTTTAAACCAATATTTATAAACCTTGTTTTTAACCAGGGCTGCGCCCTGTGCGCGTGACCGCGCACGCCGAAGGGGGGTGCCCCCCCTTCTCGAACCCTCCCGGCCCGCTAACGCGGGCCTCCCATCCCCCCAGGGGCTGCGCCCCTCGGCCGCGAACGGCCTCACCCCAAAAATGGCAGCCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCGACGGATCGATCCGGGGAATTAATTCCGGGGCAATCCCGCAAGGAGGGTGAATGAATCGGACGTTTGACCGGAAGGCATACAGGCAAGAACTGATCGACGCGGGGTTTTCCGCCGAGGATGCCGAAACCATTGCAAGCCGCACCGTCATGCGTGCGCCCCGCGAAACCTTCCAGTCCGTCGGCTCGATAGTCCAGCAAGCTACGGCCAAGATCGAGCGCGACAGCGTGCAACTGGCTCCCCCTGCCCTGCCCGCGCCATCGGCCGCCGTGGAGCGTTCGCGTCGCCTCGAACAGGAGGCGGCAGGTTTGGCGAAGTCGATGACCATCGACACGCGAGGAACTATGACGACCAAGAAGCGAAAAACCGCCGGCGAGGACCTGGCAAAACAGGTCAGCGAAGCCAAGCAGGCCGCGTTGCTGAAACACACGAAGCAGCAGATCAAGGAAATGCAGCTTTCCTTGTTCGATATTGCGCCGTGGCCGGACACGATGCGAGCCATGCCAAACGACACGGCCCGCTCTGCCCTGTTCACCACGCGCAACAAGAAAATCCCGCGCGAGGCGCTGCAAAACAAGGTCATTTTCCACGTCAACAAGGACGTGAAGATCACCTACACCGGCGTCGAGCTGCGGGCCGACGATGACGAACTGGTGTGGCAGCAAGTGTTGGAGTACGCGAAGCGCACCCCTATCGGCGAGCCGATCACCTTCACGTTCTACGAGCTTTGCCAGGACCTGGGCTGGTCGATCAATGGCCGGTATTACACGAAGGCCGAGGAATGCCTGTCGCGCCTACAGGCGACGGCCATGGGCTTCACGTCCGACCGCGTTGGGCACCTGGAATCGGTGTCGCTGCTGCACCGCTTCCGCGTCCTGGACCGTGGCAAGAAAACGTCCCGTTGCCAGGTCCTGATCGACGAGGAAATCGTCGTGCTGTTTGCTGGCGACCACTACACGAAATTCATATGGGAGAAGTACCGCAAGCTGTCGCCGACGGCCCGACGGATGTTCGACTATTTCAGCTCGCACCGGGAGCCGTACCCGCTCAAGCTGGAAACCTTCCGCCTCATGTGCGGATCGGATTCCACCCGCGTGAAGAAGTGGCGCGAGCAGGTCGGCGAAGCCTGCGAAGAGTTGCGAGGCAGCGGCCTGGTGGAACACGCCTGGGTCAATGATGACCTGGTGCATTGCAAACGCTAGGGCCTTGTGGGGTCAGTTCCGGCTGGGGGTTCAGCAGCCACCTG -pRO1600: - description: Broad host range plasmid (varies per host) for gram negative cells. - sequence: GCCGCTGGTGCCGCTGGTTGGACGCCAAGGGTGAATCCGCCTCGATACCCTGATTACTCGCTTCCTGCGCCCTCTCAGGCGGCGATAGGGGACTGGTAAAACGGGGATTGCCCAGACGCCTCCCCCGCCCCTTCAGGGGCACAAATGCGGCCCCAACGGGGCCACGTAGTGGTGCGTTTTTTGCGTTTCCACCCTTTTCTTCCTTTTCCCTTTTAAACCTTTTAGGACGTCTACAGGCCACGTAATCCGTGGCCTGTAGAGTTTAAAAAGGGACGGATTTGTTGCCATTAAGGGACGGATTTGTTGTTAAGAAGGGACGGATTTGTTGTTGTAAAGGGACGGATTTGTTGTATTGTGGGACGCAGATACAGTGTCCCCTTATACACAAGGAATGTCGAACGTGGCCTCACCCCCAATGGTTTACAAAAGCAATGCCCTGGTCGAGGCCGCGTATCGCCTCAGTGTTCAGGAACAGCGGATCGTTCTGGCCTGTATTAGCCAGGTGAAGAGGAGCGAGCCTGTCACCGATGAAGTGATGTATTCAGTGACGGCGGAGGACATAGCGACGATGGCGGGTGTCCCTATCGAATCTTCCTACAACCAGCTCAAAGAAGCGGCCCTGCGCCTGAAACGGCGGGAAGTCCGGTTAACCCAAGAGCCCAATGGCAAGGGGAAAAGACCGAGTGTGATGATTACCGGCTGGGTGCAAACAATCATCTACCGGGAGGGTGAGGGCCGTGTAGAACTCAGGTTCACCAAAGACATGCTGCCGTACCTGACGGAACTCACCAAACAGTTCACCAAATACGCCTTGGCTGACGTGGCCAAGATGGACAGCACCCACGCGATCAGGCTTTACGAGCTGCTCATGCAATGGGACAGCATCGGCCAGCGCGAAATAGAAATTGACCAGCTGCGAAAGTGGTTTCAACTGGAAGGCCGGTATCCCTCGATCAAGGACTTCAAGTTGCGAGTGCTTGATCCAGCCGTGACGCAGATCAACGAGCACAGCCCGCTACAGGTGGAGTGGGCGCAGCGAAAGACCGGGCGCAAGGTCACACATCTGTTGTTCAGTTTTGGACCGAAGAAGCCCGCCAAGGCGGTGGGTAAGGCCCCAGCGAAGCGCAAGGCCGGGAAGATTTCAGATGCTGAGATCGCGAAACAGGCTCGCCCTGGTGAGACATGGGAAGCGGCCCGCGCTCGACTAACCCAGATGCCGCTGGATCTGGCCTAGAGGCCGTGGCCACCACGGCCCGGCCTGCCTTTC -ColA: - description: Medium copy (20-40 copies) Escherichia coli origin. Compatible with pUC/pBR322, p15A, RSF1030, CloDF13, and SC101 - sequence: TGGTGTCGGGAATCCGTAAAGGATCTTCTTGAGATCCTTTTACGATCGTCGTAATCTCCTGCTCTGTAAACGAAAAAACCGCCTGGGGAGGCGGTTTGATCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCTGGTAACTGGCTTTAGTGGAGCGCAGATACCAAATACTGTCCTTTCAGTGTAGCCTCTGTTAGGCCACCACTTCAAGACTCTCGATATCTAAATCCACTAATTCTCAGTTACCAATGGCTGCTGCCAGTGGCGTTTTGTCGTGTCTTTCCGGGTTGGACTCAAGATGATAGTTACCGGATAAGGCGCAGCAGTCGGGCTGAACGGGGGGTTCTTGCACACAGCCCAGCTTGGAGCGAACTGTCTACACGGAACGGGACGTGGTGATTTGGGTAAAGCCTCCACCACAACACGGACGCCGCAGGACGGGAACAGGAGAGCGCAAGAGGGAGCCATCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGTTCGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTCGCTCCGGCCTTATTGTCTCTCTGCTAAGTATCCTCCTGGCATCTTCTAGGACGTTT -BAC: - description: Single copy origin built to create Bacterial Artificial Chromosomes, which allow for cloning and stable maintenance of very large DNA fragments (up to 300kb) - sequence: CATGGAGCGGCGTAACCGTCGCACAGGAAGGACAGAGAAAGCGCGGATCTGGGAAGTGACGGACAGAACGGTCAGGACCTGGATTGGGGAGGCGGTTGCCGCCGCTGCTGCTGACGGTGTGACGTTCTCTGTTCCGGTCACACCACATACGTTCCGCCATTCCTATGCGATGCACATGCTGTATGCCGGTATACCGCTGAAAGTTCTGCAAAGCCTGATGGGACATAAGTCCATCAGTTCAACGGAAGTCTACACGAAGGTTTTTGCGCTGGATGTGGCTGCCCGGCACCGGGTGCAGTTTGCGATGCCGGAGTCTGATGCGGTTGCGATGCTGAAACAATTATCCTGAGAATAAATGCCTTGGCCTTTATATGGAAATGTGGAACTGAGTGGATATGCTGTTTTTGTCTGTTAAACAGAGAAGCTGGCTGTTATCCACTGAGAAGCGAACGAAACAGTCGGGAAAATCTCCCATTATCGTAGAGATCCGCATTATTAATCTCAGGAGCCTGTGTAGCGTTTATAGGAAGTAGTGTTCTGTCATGATGCCTGCAAGCGGTAACGAAAACGATTTGAATATGCCTTCAGGAACAATAGAAATCTTCGTGCGGTGTTACGTTGAAGTGGAGCGGATTATGTCAGCAATGGACAGAACAACCTAATGAACACAGAACCATGATGTGGTCTGTCCTTTTACAGCCAGTAGTGCTCGCCGCAGTCGAGCGACAGGGCGAAGCCCTCGAGTGAGCGAGGAAGCACCAGGGAACAGCACTTATATATTCTGCTTACACACGATGCCTGAAAAAACTTCCCTTGGGGTTATCCACTTATCCACGGGGATATTTTTATAATTATTTTTTTTATAGTTTTTAGATCTTCTTTTTTAGAGCGCCTTGTAGGCCTTTATCCATGCTGGTTCTAGAGAAGGTGTTGTGACAAATTGCCCTTTCAGTGTGACAAATCACCCTCAAATGACAGTCCTGTCTGTGACAAATTGCCCTTAACCCTGTGACAAATTGCCCTCAGAAGAAGCTGTTTTTTCACAAAGTTATCCCTGCTTATTGACTCTTTTTTATTTAGTGTGACAATCTAAAAACTTGTCACACTTCACATGGATCTGTCATGGCGGAAACAGCGGTTATCAATCACAAGAAACGTAAAAATAGCCCGCGAATCGTCCAGTCAAACGACCTCACTGAGGCGGCATATAGTCTCTCCCGGGATCAAAAACGTATGCTGTATCTGTTCGTTGACCAGATCAGAAAATCTGATGGCACCCTACAGGAACATGACGGTATCTGCGAGATCCATGTTGCTAAATATGCTGAAATATTCGGATTGACCTCTGCGGAAGCCAGTAAGGATATACGGCAGGCATTGAAGAGTTTCGCGGGGAAGGAAGTGGTTTTTTATCGCCCTGAAGAGGATGCCGGCGATGAAAAAGGCTATGAATCTTTTCCTTGGTTTATCAAACGTGCGCACAGTCCATCCAGAGGGCTTTACAGTGTACATATCAACCCATATCTCATTCCCTTCTTTATCGGGTTACAGAACCGGTTTACGCAGTTTCGGCTTAGTGAAACAAAAGAAATCACCAATCCGTATGCCATGCGTTTATACGAATCCCTGTGTCAGTATCGTAAGCCGGATGGCTCAGGCATCGTGTCTCTGAAAATCGACTGGATCATAGAGCGTTACCAGCTGCCTCAAAGTTACCAGCGTATGCCTGACTTCCGCCGCCGCTTCCTGCAGGTCTGTGTTAATGAGATCAACAGCAGAACTCCAATGCGCCTCTCATACATTGAGAAAAAGAAAGGCCGCCAGACGACTCATATCGTATTTTCCTTCCGCGATATCACTTCCATGACGACAGGATAGTCTGAGGGTTATCTGTCACAGATTTGAGGGTGGTTCGTCACATTTGTTCTGACCTACTGAGGGTAATTTGTCACAGTTTTGCTGTTTCCTTCAGCCTGCATGGATTTTCTCATACTTTTTGAACTGTAATTTTTAAGGAAGCCAAATTTGAGGGCAGTTTGTCACAGTTGATTTCCTTCTCTTTCCCTTCGTCATGTGACCTGATATCGGGGGTTAGTTCGTCATCATTGATGAGGGTTGATTATCACAGTTTATTACTCTGAATTGGCTATCCGCGTGTGTACCTCTACCTGGAGTTTTTCCCACGGTGGATATTTCTTCTTGCGCTGAGCGTAAGAGCTATCTGACAGAACAGTTCTTCTTTGCTTCCTCGCCAGTTCGCTCGCTATGCTCGGTTACACGGCTGCGGCGAGCGCTAGTGATAATAAGTGACTGAGGTATGTGCTCTTCTTATCTCCTTTTGTAGTGTTGCTCTTATTTTAAACAACTTTGCGGTTTTTTGATGACTTTGCGATTTTGTTGTTGCTTTGCAGTAAATTGCAAGATTTAATAAAAAAACGCAAAGCAATGATTAAAGGATGTTCAGAATGAAACTCATGGAAACACTTAACCAGTGCATAAACGCTGGTCATGAAATGACGAAGGCTATCGCCATTGCACAGTTTAATGATGACAGCCCGGAAGCGAGGAAAATAACCCGGCGCTGGAGAATAGGTGAAGCAGCGGATTTAGTTGGGGTTTCTTCTCAGGCTATCAGAGATGCCGAGAAAGCAGGGCGACTACCGCACCCGGATATGGAAATTCGAGGACGGGTTGAGCAACGTGTTGGTTATACAATTGAACAAATTAATCATATGCGTGATGTGTTTGGTACGCGATTGCGACGTGCTGAGGACGTATTTCCACCGGTGATCGGGGTTGCTGCCCATAAAGGTGGCGTTTACAAAACCTCAGTTTCTGTTCATCTTGCTCAGGATCTGGCTCTGAAGGGGCTACGTGTTTTGCTCGTGGAAGGTAACGACCCCCAGGGAACAGCCTCAATGTATCACGGATGGGTACCAGATCTTCATATTCATGCAGAAGATACTCTCCTGCCTTTCTATCTTGGGGAAAAGGACGATGTCACTTATGCAATAAAGCCCACTTGCTGGCCGGGGCTTGACATTATTCCTTCCTGTCTGGCTCTGCACCGTATTGAAACTGAGTTAATGGGCAAATTTGATGAAGGTAAACTGCCCACCGATCCACACCTGATGCTCCGACTGGCCATTGAAACTGTTGCTCATGACTATGATGTCATAGTTATTGACAGCGCGCCTAACCTGGGTATCGGCACGATTAATGTCGTATGTGCTGCTGATGTGCTGATTGTTCCCACGCCTGCTGAGTTGTTTGACTACACCTCCGCACTGCAGTTTTTCGATATGCTTCGTGATCTGCTCAAGAACGTTGATCTTAAAGGGTTCGAGCCTGATGTACGTATTTTGCTTACCAAATACAGCAATAGTAATGGCTCTCAGTCCCCGTGGATGGAGGAGCAAATTCGGGATGCCTGGGGAAGCATGGTTCTAAAAAATGTTGTACGTGAAACGGATGAAGTTGGTAAAGGTCAGATCCGGATGAGAACTGTTTTTGAACAGGCCATTGATCAACGCTCTTCAACTGGTGCCTGGAGAAATGCTCTTTCTATTTGGGAACCTGTCTGCAATGAAATTTTCGATCGTCTGATTAAACCACGCTGGGAGATTAGATAATGAAGCGTGCGCCTGTTATTCCAAAACATACGCTCAATACTCAACCGGTTGAAGATACTTCGTTATCGACACCAGCTGCCCCGATGGTGGATTCGTTAATTGCGCGCGTAGGAGTAATGGCTCGCGGTAATGCCATTACTTTGCCTGTATGTGGTCGGGATGTGAAGTTTACTCTTGAAGTGCTCCGGGGTGATAGTGTTGAGAAAACCTCTCGGGTATGGTCAGGTAATGAACGTGACCAGGAGCTGCTTACTGAGGACGCACTGGATGATCTCATCCCTTCTTTTCTACTGACTGGTCAACAGACACCGGCGTTCGGTCGAAGAGTATCTGGTGTCATAGAAATTGCCGATGGGAGTCGCCGTCGTAAAGCTGCTGCACTTACCGAAAGTGATTATCGTGTTCTGGTTGGCGAGCTGGATGATGAGCAGATGGCTGCATTATCCAGATTGGGTAACGATTATCGCCCAACAAGTGCTTATGAACGTGGTCAGCGTTATGCAAGCCGATTGCAGAATGAATTTGCTGGAAATATTTCTGCGCTGGCTGATGCGGAAAATATTTCACGTAAGATTATTACCCGCTGTATCAACACCGCCAAATTGCCTAAATCAGTTGTTGCTCTTTTTTCTCACCCCGGTGAACTATCTGCCCGGTCAGGTGATGCACTTCAAAAAGCCTTTACAGATAAAGAGGAATTACTTAAGCAGCAGGCATCTAACCTTCATGAGCAGAAAAAAGCTGGGGTGATATTTGAAGCTGAAGAAGTTATCACTCTTTTAACTTCTGTGCTTAAAACGTCATCTGCATCAAGAACTAGTTTAAGCTCACGACATCAGTTTGCTCCTGGAGCGACAGTATTGTATAAGGGCGATAAAATGGTGCTTAACCTGGACAGGAGCCGTGTTCCAACTGAGTGTATAGAGAAAATTGAGGCCATTCTTAAGGAACTTGAAAAGCCAGCACCCTGATGCGACCACGTTTTAGTCTACGTTTATCTGTCTTTACTTAATGTCCTTTGTTACAGGCCAGAAAGCATAACTGGCCTGAATATTCTCTCTGGGCCCACTGTTCCACTTGTATCGTCGGTCTGATAATCAGACTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATAATCAGACTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCATGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGAACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCACGATCCCACTCGTGTTGTCGGTCTGATTATCGGTCTGGGACCACGGTCCCACTTGTATTGTCGATCAGACTATCAGCGTGAGACTACGATTCCATCAATGCCTGTCAAGGGCAAGTATTGACATGTCGTCGTAACCTGTAGAACGGAGTAACCTCGGTGTGCGGTTGTATGCCTGCTGTGGATTGCTGCTGTGTCCTGCTTATCCACAACATTTTGCGCACGGTTATGTGGACAAAATACCTGGTTACC -R6K: - description: Medium copy (15-30 copies) or high copy depending on Pi expression. Requires Pi protein. Orthogonal to ColE2. - sequence: GATCTGAAGATCAGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCCTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGG -CloDF13: - description: Medium copy (20-40 copies) Escherichia coli origin. Compatible with pUC/pBR322, p15A, RSF1030, ColA, and SC101 - sequence: GATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTTTTGCCCTGTAAACGAAAAAACCACCTGGGGAGGTGGTTTGATCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCGTGGTAACTGGCTTTCGCAGAGCACAGCAACCAAATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTCAAGAGCAACCGCGTGTTTAGCTAAACAAATCCTCTGCGAACTCCCAGTTACCAATGGCTGCTGCCAGTGGCGTTTTACCGTGCTTTTCCGGGTTGGACTCAAGTGAACAGTTACCGGATAAGGCGCAGCAGTCGGGCTGAACGGGGAGTTCTTGCTTACAGCCCAGCTTGGAGCGAACGACCTACACCGAGCCGAGATACCAGTGTGTGAGCTATGAGAAAGCGCCACACTTCCCGTAAGGGAGAAAGGCGGAACAGGTATCCGGTAAACGGCAGGGTCGGAACAGGAGAGCGCAAGAGGGAGCGACCCGCCGGAAACGGTGGGGATCTTTAAGTCCTGTCGGGTTTCGCCCGTACTGTCAGATTCATGGTTGAGCCTCACGGCTCCCACAGATGCACCGGAAAAGCGTCTGTTTATGTGAACTCTGGCAGGAGGGCGGAGCCTATGGAAAAACGCCACCGGCGCGGCCCTGCTGTTTTGCCTCACATGTTAGTCCCCTGCTTATCCACGGAATCTGTGGGTAACTTTGTATGTGTCCGCAGCGC -pLG13: - description: pLG13 is a ColE1 family plasmid that natively contains the EcoRV restriction-modification system - sequence: TTGAGATCCTTTTTTTTGCGCGCAATCTGCTGTCTGTAGACGAAAAAACCACCCTGGTAGGTGGTTTTTCGAAGGTTAAATAATCCTGGCAGATTATTTAACCGTGGTAACAGGGTGTACAAGACCGCTGCCACCAAATCTGTCCTTTCAGTGTAGCCGCAGTTGGTCCTTCACTTCAAGAACTACGTATCAGCAATTCTTGTACATCCTCTACCAGTGGCTGCTGCCAGTGGCGTTAAGGCGTGACTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCAACCCGAAGGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGGTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA -pSCANS-inducible: - description: Inducible copy number vector. Sourced from pOpen_v4 - sequence: agagcttggcaggtgtcgcgtatgcatcgatcacgtgtccacgttctttaatagtggactcttgttccaaactggaacaacactcggatcgatccggcgcgcaccgtgggaaaaactccaggtagaggtacacacgcggatagccaattcagagtaataaactgtgataatcaaccctcatcaatgatgacgaactaacccccgatatcaggtcacatgacgaagggaaagagaaggaaatcaactgtgacaaactgccctcaaatttggcttccttaaaaattacagttcaaaaagtatgagaaaatccatgcaggctgaaggaaacagcaaaactgtgacaaattaccctcagtaggtcagaacaaatgtgacgaaccaccctcaaatctgtgacagataaccctcagactatcctgtcgtcatggaagtgatatcgcggaaggaaaatacgatatgagtcgtctggcggcctttctttttctcaatgtatgagaggcgcattggagttctgctgttgatctcattaacacagacttgcaggaagcggcggcggaagtcaggcatacgctggtaactttgaggcagctggtaacgctctatgatccagtcgattttcagagacacgatgcctgagccatccggcttacgatactgacacagggattcgtataaacgcatggcatacggattggtgatttcttttgtttcactaagccgaaactgcgtaaaccggttctgtaacccgataaagaagggaatgagatatgggttgatatgtacactgtaaagccctctggatggactgtgcgcacgtttgataaaccaaggaaaagattcatagcctttttcatcaccggcatcctcttcagggcgataaaaaaccacttccttccccgcgaaactcttcaatgcctgccgtatatccttactggcttccgcagaggtcaatccgaatatttcagcatatttagcaacatggatctcgcagataccgtcatgttcctgtagggtgccatcagattttctgatctggtcaacgaacagatacagcatacgtttttgatcccgggagagactatatgccgcctcagtgaggtcgtttgactggacgattcgcgggctatttttacgtttcttgtgattgataaccgctgtttccgccatgacagatccatgtgaagtgtgacaagtttttagattgtcacactaaataaaaaagagtcaataagcagggataactttgtgaaaaaacagcttcttctgagggcaatttgtcacagggttaagggcaatttgtcacagacaggactgtcatttgagggtgatttgtcacactgaaagggcaatttgtcacaacaccttctgtagaaccagcatggataaaggcctacaaggcgctctaaaaaagaagatctaaaaactataaaaaaaataattataaaaatatccccgtggataagtggataaccccaagggaagttttttcaggcatcgtgtgtaagcagaatatataagtgctgttccctggtgcttcctcgctcactcgaaattcccggggatagctttatgcttgtaaaccgttttgtgaaaaaatttttaaaataaaaaaggggacctctagggtccccaattaattagtaatataatctattaaaggtcattcaaaaggtcatccaccggatcaattcccctgctcgcgcaggctgggtgccaagctctcgggtaacatcaaggcccgatccttggagcccttgccctcccgcacgatgatcgtgccgtgatcgaaatccagatccttgacccgcagttgcaaaccctcactgatccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcggtacctgtcgcggcaacgcgctaacagacgtagtaagaaccaccagcattgtaatgctggctaaagtcactttcctgagctgtataacgatgagcgattttactttttctggctatgaattggcctgctttgtaacacactccggtctatcccgtagcgccgggcatatcctgtcgcaatgtgcaaatctcgcggcaacaaccagtgaatacttcattcacaagcctcaccgcctgatcgcggcagaaactggttatagccaatcaaccgtcgttcgtgcattccgtgaagctgtaaacaaaggtatcctgtctgtagagattgttatcggcgatcaccgtgaacgtcgcgctaacctgtaccggtttacaccatcctttttggccttcgcacaacaagccaaaaatgcgctgatagaaagcaaattaaagatctcttcagcggcaaccaaggttaaagctgttctcgctaagacattggctttatttaattttttatccacacccccatgtcaaaatgataccccctccccctgtcaggatgacgtggcaataaagaataagaagtcacaagttaaaaaaacaaaaagatcagtttccggcggtgccggaacaaccagcctcaaaaaattgacttcatggatcgctaaggcaaaagcaaaggctgacaatctgcggttatccaaaaaacgcactcaaaaacatgagttcaagcagaaagtagaggcggctgcgcggaaatatgcttacctgaagaacaagcgttcgcctgatattggcgggatatcaaacttcgataacctaccgcattgcatgacggtaaacgaagctcttaatgcggttttagccaaaaataaagataacgaacaatggggtataccggcaggattcagagggtaatgaattgctctaattataaccatgcatactttcaacacctctagtttgccatgaggcaaactcataggtgtcctggtaagaggacactgttgccaaaactggacgcc diff --git a/parts/parts/ecoli/promoters.yaml b/parts/parts/ecoli/promoters.yaml deleted file mode 100644 index e6f5a49..0000000 --- a/parts/parts/ecoli/promoters.yaml +++ /dev/null @@ -1,101 +0,0 @@ -# Anderson -BBa_J23119: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ttgacagctagctcagtcctaggtataatgctagc -BBa_J23100: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ttgacggctagctcagtcctaggtacagtgctagc -BBa_J23101: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: tttacagctagctcagtcctaggtattatgctagc -BBa_J23102: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ttgacagctagctcagtcctaggtactgtgctagc -BBa_J23103: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ctgatagctagctcagtcctagggattatgctagc -BBa_J23104: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ttgacagctagctcagtcctaggtattgtgctagc -BBa_J23105: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: tttacggctagctcagtcctaggtactatgctagc -BBa_J23106: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: tttacggctagctcagtcctaggtatagtgctagc -BBa_J23107: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: tttacggctagctcagccctaggtattatgctagc -BBa_J23108: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ctgacagctagctcagtcctaggtataatgctagc -BBa_J23109: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: tttacagctagctcagtcctagggactgtgctagc -BBa_J23110: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: tttacggctagctcagtcctaggtacaatgctagc -BBa_J23111: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ttgacggctagctcagtcctaggtatagtgctagc -BBa_J23112: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ctgatagctagctcagtcctagggattatgctagc -BBa_J23113: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ctgatggctagctcagtcctagggattatgctagc -BBa_J23114: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: tttatggctagctcagtcctaggtacaatgctagc -BBa_J23115: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: tttatagctagctcagcccttggtacaatgctagc -BBa_J23116: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ttgacagctagctcagtcctagggactatgctagc -BBa_J23117: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ttgacagctagctcagtcctagggattgtgctagc -BBa_J23118: - prefix: TACA - suffix: AACT - tags: ['promoter', 'anderson_promoter'] - sequence: ttgacggctagctcagtcctaggtattgtgctagc diff --git a/parts/parts/vnat.yaml b/parts/parts/vnat.yaml index 7664aba..000a629 100644 --- a/parts/parts/vnat.yaml +++ b/parts/parts/vnat.yaml @@ -1,178 +1,338 @@ #https://doi.org/10.1021/acssynbio.1c00126 -dummy_promoter: + +## Anderson +BBa_J23119: prefix: TACA suffix: AACT - sequence: N + tags: ['promoter', 'anderson_promoter'] + sequence: ttgacagctagctcagtcctaggtataatgctagc +BBa_J23100: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ttgacggctagctcagtcctaggtacagtgctagc +BBa_J23101: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: tttacagctagctcagtcctaggtattatgctagc +BBa_J23102: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ttgacagctagctcagtcctaggtactgtgctagc +BBa_J23103: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ctgatagctagctcagtcctagggattatgctagc +BBa_J23104: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ttgacagctagctcagtcctaggtattgtgctagc +BBa_J23105: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: tttacggctagctcagtcctaggtactatgctagc +BBa_J23106: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: tttacggctagctcagtcctaggtatagtgctagc +BBa_J23107: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: tttacggctagctcagccctaggtattatgctagc +BBa_J23108: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ctgacagctagctcagtcctaggtataatgctagc +BBa_J23109: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: tttacagctagctcagtcctagggactgtgctagc +BBa_J23110: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: tttacggctagctcagtcctaggtacaatgctagc +BBa_J23111: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ttgacggctagctcagtcctaggtatagtgctagc +BBa_J23112: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ctgatagctagctcagtcctagggattatgctagc +BBa_J23113: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ctgatggctagctcagtcctagggattatgctagc +BBa_J23114: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: tttatggctagctcagtcctaggtacaatgctagc +BBa_J23115: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: tttatagctagctcagcccttggtacaatgctagc +BBa_J23116: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ttgacagctagctcagtcctagggactatgctagc +BBa_J23117: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ttgacagctagctcagtcctagggattgtgctagc +BBa_J23118: + prefix: TACA + suffix: AACT + tags: ['promoter', 'anderson_promoter'] + sequence: ttgacggctagctcagtcctaggtattgtgctagc +# Non-Anderson +Vnat_dummy_promoter: + prefix: TACA + suffix: AACT + sequence: cccctggcgcccctt Ptrc_promoter: prefix: TACA suffix: AACT - sequence: N + sequence: gtctagggcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcagcgggtcgactcccctaggtcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgacacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctatcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccatcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccaaagctttccctcgacaattcgataaatgtgagcggataacattgacattggtgagcggataacaagatactgagcacatcagcaggacgcactgacc Ptet_promoter: prefix: TACA suffix: AACT - sequence: N + sequence: ttttgttatcaataaaaaaggccccccgttagggaggcttattgttcgtcccatcacggaaaaaggttatgctgcttttaagacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctgctctacacctagcctctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctgctaatcactctacttttatctaatcgagacatcattaattcctaatttttgttgacactctatcattgatagagttattttaccactccctatcagtgatagagaaaagtgaa Para(Vn)_promoter: prefix: TACA suffix: AACT - sequence: N + sequence: tgttatccatccactggtagaggtgagtgttcgctatacatatttgtggatttagggacatttgttagtgacaaaaatcacagcggaaaaatgtagcgaatttgtccattcatttagccagtgtggctatgacacagatctcaattatgcgaccaatgatccaaattctcagtaagcaacccaataccagcctaatgcaaacgttgaattcgctggtttcttgcttttccgacctgacagaagaaggtgttaaaaagaacacaaaaaatcgtccatgacgtttttgtccataaagttagcagacctctttatgggataacatccctcctctagctataacaacaagtagatttagtttgctgaccaaaa Prham(Vn)_promoter: prefix: TACA suffix: AACT - sequence: N + sequence: gacacactctaataaccaagccccgcaattcgcggggcttattatttttagccagccaaatgttacgccctccccgttatttcaaacagtaaatagcttgaataaataagaaaaaccacaccttttacacgcctactccacttcacttaaacccaggttttatctggcctcacgcacgagttgtcaaaagtttgaattaccgcaagagcttcttgagaaaaacgcatcgaatacgttttttcagggggatttttgaagttatttagtgcggaaaaacggtgtaatacctctaaagaacaagaggtgttaatc # dropout sfGFP / mScarlet for each +Vnat_dummy_rbs: + prefix: AACT + suffix: AATG + sequence: agagtgtcaggatacccgataatc B0029_rbs: prefix: AACT suffix: AATG - sequence: N + sequence: agagttcacacaggaaacctaatc B0030_rbs: prefix: AACT suffix: AATG - sequence: N + sequence: agagattaaagaggagaaataatc B0031_rbs: prefix: AACT suffix: AATG - sequence: N + sequence: agagtcacacaggaaacctaatc B0032_rbs: prefix: AACT suffix: AATG - sequence: N + sequence: agagtcacacaggaaagtaatc B0033_rbs: prefix: AACT suffix: AATG - sequence: N + sequence: agagtcacacaggactaatc B0034_rbs: prefix: AACT suffix: AATG - sequence: N + sequence: agagaaagaggagaaataatc B0035_rbs: prefix: AACT suffix: AATG - sequence: N + sequence: agagattaaagaggagaataatc B0064_rbs: prefix: AACT suffix: AATG - sequence: N + sequence: agagaaagaggggaaataatc # cds lux_operon(Vn): prefix: A - suffix: ATCC - sequence: N + suffix: GGATCC + sequence: atgactaaaaaaatttcattcattattaacggccaggttgaaatctttcccgaaagtgatgatttagtgcaatccattaattttggtgataatagtgtttacctgccaatattgaatgactctcatgtaaaaaacattattgattgtaatggaaataacgaattacggttgcataacattgtcaattttctctatacggtagggcaaagatggaaaaatgaagaatactcaagacgcaggacatacattcgtgacttaaaaaaatatatgggatattcagaagaaatggctaagctagaggccaattggatatctatgattttatgttctaaaggcggcctttatgatgttgtagaaaatgaacttggttctcgccatatcatggatgaatggctacctcaggatgaaagttatgttcgggcttttccgaaaggtaaatctgtacatctgttggcaggtaatgttccattatctgggatcatgtctatattacgcgcaattttaactaagaatcagtgtattataaaaacatcgtcaaccgatccttttaccgctaatgcattagcgttaagttttattgatgtagaccctaatcatccgataacgcgctctttatctgttatatattggccccaccaaggtgatacatcactcgcaaaagaaattatgcgacatgcggatgttattgtcgcttggggagggccagatgcgattaattgggcggtagagcacgcgccatcttatgctgatgtgattaaatttggttctaaaaagagtctttgcattatcgataatcctgttgatttgacgtccgcagcgacaggtgcggctcatgatgtttgtttttacgatcagcgagcttgtttttctgcccaaaacatatattacatgggaaatcattatgaggaatttaagttagcgttgatagaaaaacttaatctatatgcgcatatattaccgaatgccaaaaaagattttgatgaaaaggcggcctattctttagttcaaaaagaaagcctgtttgctggattaaaagtagaggtggatattcatcaacgttggatgattattgagtcaaatgcaggtgtggaatttaatcaaccacttggcagatgtgtgtaccttcatcacgtcgataatattgagcaaatattgccttatgttcaaaaaaataagacgcaaaccatatctatttttccttgggagtcatcatttaaatatcgagatgcgttagcattaaaaggtgcggaaaggattgtagaagcaggaatgaataacatatttcgagttggtggatctcatgacggaatgaggccgttgcaacgattagtgacatatatttctcatgaaaggccatctaactatacggctaaggatgttgcggttgaaatagaacagactcgattcctggaagaagataagttccttgtatttgtcccataataggtaaaaagtatggaaaatgaatcaaaatataaaaccatcgaccacgttatttgtgttgaaggaaataaaaaaattcatgtttgggaaacgctgccagaagaaaacagcccaaagagaaagaatgccattattattgcgtctggttttgcccgcaggatggatcattttgctggtctggcggaatatttatcgcggaatggatttcatgtgatccgctatgattcgcttcaccatgttggcttgagttcagggacaattgatgaatttacaatgtctataggaaagcagagcttgttagcagtggttgattggttaactacacgaaaaataaataacttcggtatgttggcttccagcttatctgcgcggatagcttatgcaagcctatctgaaatcaatgcttcgtttttaatcaccgcagtcggtgttgttaacttaagatattctcttgaaagagctttagggtttgattatctcagtctacccattaatgaattgccggataatctggattttgaaggccataaattgggtgctgaagtctttgcgagagattgtcttgattttggttgggaagatttagcttctacaattaataacatgatgtatcttgatataccgtttattgcttttactgcaaataacgataattgggtcaagcaagatgaagttatcacattgttatcaaatattcgtagtaatcgatgcaagatatattctttgttaggaagttcgcatgacttgagtgaaaatttagtggtcctgcgcaatttttatcaatcggttacgaaagccgctatcgcgatggataatgatcatctggatattgatgttgatattactgaaccgtcatttgaacatttaactattgcgacagtcaatgaacgccgaatgagaattgagattgaaaatcaagcaatttctctgtcttaaaatctattgagatattctatcactcaaatagcaatataaggactctctatgaaatttggaaactttttgcttacataccaacctccccaattttctcaaacagaggtaatgaaacgtttggttaaattaggtcgcatctctgaggagtgtggttttgataccgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggcgcgactaaaaaattgaatgtaggaactgccgctattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgagggctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcgcgccttagcggaatgctggtacgggctgataaagaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaaccccgcggcgtatagcagaggtggcgcaccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctcaatttggcctaccgatgatattaagttggattataaatactaacgaaaagaaagcacaacttgagctttataatgaagtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtagatcatgactcaattaaagcgaaagagatttgccggaaatttctggggcattggtatgattcttatgtgaatgctacgactatttttgatgattcagaccaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgtattgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtattgacataattcaaaaagacattgatgctacaggaatatcaaatatttgttgtggatttgaagctaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgctattatattagctaaggagaaagaaatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgtatgcaggaaataacggagtatgttgataagttgaattttgaacagattttagtgtatgaaaatcatttttcagataatggtgttgtcggcgctcctctgactgtttctggttttctgctcggtttaacagagaaaattaaaattggttcattaaatcacatcattacaactcatcatcctgtccgcatagcggaggaagcgtgcttattggatcagttaagtgaagggagatttattttagggtttagtgattgcgaaaaaaaagatgaaatgcatttttttaatcgcccggttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatccagataacgatttttatagcttccctaaaatatctgtaaatccccatgcttatacgccaggcggacctcggaaatatgtaacagcaaccagtcatcatattgttgagtgggcggccaaaaaaggtattcctctcatctttaagtgggatgattctaatgatgttagatatgaatatgctgaaagatataaagccgttgcggataaatatgacgttgacctatcagagatagaccatcagttaatgatattagttaactataacgaagatagtaataaagctaaacaagagactcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaatttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaattatacggagtgtataactgcggctaagttggcaattgaaaagtgtggtgcgaaaagtgtattgctgtcctttgaaccaatgaatgatttgatgagccaaaaaaatgtaatcaatattgttgatgataatattaagaagtaccacatggaatatacctaatagatttcgagttgcagcgaggcggcaagtgaacgaatccccaggagcatagataactatgtgactggggtgagtgaaagcagccaacaaagcagcagcttgaaagatgaagggtataaaagagtatgacagcagtgctgccatactttctaatattatcttgaggagtaaaacaggtatgacttcatatgttgataaacaagaaattacagcaagctcagaaattgatgatttgattttttcgagcgatccattagtgtggtcttacgacgagcaggaaaaaatcagaaagaaacttgtgcttgatgcatttcgtaatcattataaacattgtcgagaatatcgtcactactgtcaggcacacaaagtagatgacaatattacggaaattgatgacatacctgtattcccaacatcggtttttaagtttactcgcttattaacttctcaggaaaacgagattgaaagttggtttaccagtagcggcacgaatggtttaaaaagtcaggtggcgcgtgacagattaagtattgagagactcttaggctctgtgagttatggcatgaaatatgttggtagttggtttgatcatcaaatagaattagtcaatttgggaccagatagatttaatgctcataatatttggtttaaatatgttatgagtttggtggaattgttatatcctacgacatttaccgtaacagaagaacgaatagattttgttaaaacattgaatagtcttgaacgaataaaaaatcaagggaaagatctttgtcttattggttcgccatactttatttatttactctgccattatatgaaagataaaaaaatctcattttctggagataaaagcctttatatcataaccggaggcggctggaaaagttacgaaaaagaatctctgaaacgtgatgatttcaatcatcttttatttgatactttcaatctcagtgatattagtcagatccgagatatatttaatcaagttgaactcaacacttgtttctttgaggatgaaatgcagcgtaaacatgttccgccgtgggtatatgcgcgagcgcttgatcctgaaacgttgaaacctgtacctgatggaacgccggggttgatgagttatatggatgcgtcagcaaccagttatccagcatttattgttaccgatgatgtcgggataattagcagagaatatggtaagtatcccggcgtgctcgttgaaattttacgtcgcgtcaatacgaggacgcagaaagggtgtgctttgagcttaaccgaagcgtttgatagt azurite(Vn): prefix: A - suffix: ATCC - sequence: N + suffix: GGATCC + sequence: atgtctaaaggtgaagaactgtttaccggtgttgttccaattctggttgaactggatggtgatgttaacggtcacaaattttctgtttctggtgaaggcgaaggtgatgcaacctacggtaaactgaccctgaaatttatctgtaccactggtaaactgccagttccatggccaactctggttaccactctgtctcacggtgttcaatgtttttctcgttacccagatcacatgaaacagcacgattttttcaaaagcgcaatgccagaaggttacgttcaagaacgtaccatcttcttcaaagatgacggtaactacaaaacccgtgcggaagtgaaatttgaaggtgataccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaggatggcaacattctgggtcacaaactggaatacaactttaactctcacaacatctacatcatggcggacaaacaaaaaaacggcatcaaagtgaacttcaagattcgccacaacatcgaagatggttctgttcaactggcagatcactaccaacaaaacaccccaattggtgatggtccagttctgctgccagataaccactacctgtctacccaatctgcactgtctaaagatccaaacgaaaaacgtgatcacatggtgctgctggaatttcgtaccgcagcaggtattacccacggtatggatgaactgtacaaa mTurquoise(Vn): prefix: A - suffix: ATCC - sequence: N + suffix: GGATCC + sequence: atggtttctaaaggtgaagaactgtttaccggtgttgttccaattctggttgaactggatggtgatgttaacggtcacaaattttctgtttctggtgaaggcgaaggtgatgcaacctacggtaaactgaccctgaaatttatctgtaccactggtaaactgccagttccatggccaactctggttaccactctgtcttggggtgttcaatgttttgcacgttacccagatcacatgaaacaacacgattttttcaaaagcgcaatgccagaaggttacgttcaagaacgtaccatcttcttcaaagatgacggtaactacaaaacccgtgcggaagtgaaatttgaaggtgataccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaggatggcaacattctgggtcacaaactggaatacaactactttagcgataacgtgtacatcaccgcagataaacaaaaaaacggtatcaaggcgaacttcaaaatccgtcacaacattgaagatggtggtgttcaactggcagatcactaccaacaaaacactccaattggtgatggtccagttctgctgccagataaccactacctgtctacccaatctaaactgtctaaagacccaaacgaaaaacgtgatcacatggttctgctggaatttgttaccgcagcaggtattaccttaggtatggatgaactgtacaaa sfGFP(Vn): prefix: A - suffix: ATCC - sequence: N + suffix: GGATCC + sequence: atgcgtaaaggtgaagaactgtttaccggtgttgttccaattctggttgaactggatggtgatgttaacggtcacaaattttctgttcgtggtgaaggcgaaggtgatgcaaccaacggtaaactgaccctgaaatttatctgtaccactggtaaactgccagttccatggccaactctggttaccactctgacctacggtgttcaatgttttgcacgttacccagatcacatgaaacaacacgattttttcaaaagcgcaatgccagaaggttacgttcaagaacgtaccatctcttttaaagatgacggcacctacaaaacccgtgcggaagttaaatttgaaggtgataccctggttaaccgcattgaactgaaaggcatcgattttaaagaagatggtaacatcctgggccacaaactggaatacaactttaactctcacaacgtgtacatcaccgcagacaaacaaaaaaacggtatcaaagcgaacttcaagatccgtcacaacgttgaagatggttctgttcaactggcagatcactaccaacaaaacaccccaattggtgatggtccagttctgctgccagataaccactacctgtctacccaaagcgttctgtctaaagatccaaacgaaaaacgtgatcacatggtgctgctggaatttgttaccgcagcaggtattacccacggtatggatgaactgtacaaagca mVenus(Vn): prefix: A - suffix: ATCC - sequence: N + suffix: GGATCC + sequence: atggtttctaaaggtgaagaactgtttaccggtgttgttccaattctggttgaactggatggtgatgttaacggtcacaaattttctgtttctggtgaaggcgaaggtgatgcaacctacggtaaactgaccctgaaactgatttgtaccactggtaaactgccagttccatggccaactctggttaccaccttaggttacggtctgcaatgttttgcacgttacccagatcacatgaaacaacacgattttttcaaaagcgcaatgccagaaggttacgttcaagaacgtaccatcttcttcaaagatgacggtaactacaaaacccgtgcggaagtgaaatttgaaggtgataccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaggatggcaacattctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgcagataaacaaaaaaacggcatcaaagcgaacttcaaaatccgtcacaacattgaagatggtggtgttcaactggcagatcactaccaacaaaacaccccaattggtgatggtccagttctgctgccagataaccactacctgtcttaccaatctaaactgagcaaagacccaaacgaaaaacgtgatcacatggttctgctggaatttgttaccgcagcaggtattaccttaggtatggatgaactgtacaaa mScarlet-I(Vn): prefix: A - suffix: ATCC - sequence: N + suffix: GGATCC + sequence: atggtttctaaaggtgaagcagtgatcaaagaatttatgcgcttcaaagttcacatggaaggttctatgaacggccacgaatttgaaattgaaggtgaaggcgaaggtcgtccatacgaaggtactcaaaccgcaaaactgaaagttaccaaaggtggtccactgccattttcttgggatattctgtctccacaatttatgtacggttctcgtgcatttatcaaacacccagcagatattccagactactacaaacaatcttttccggaaggtttcaaatgggaacgtgttatgaattttgaagatggtggtgcagttacggttacccaagatacctctctggaagatggtactctgatctacaaagttaaactgcgtggtactaactttccaccagatggtccagttatgcagaaaaaaaccatgggttgggaagcatctaccgaacgtctgtacccagaagatggcgttctgaaaggtgatatcaaaatggcactgcgtctgaaagatggcggtcgttacctggcagatttcaaaaccacctacaaagcgaaaaaaccagttcaaatgccaggtgcatacaacgttgatcgtaaactggatattaccagccacaacgaagattacaccgttgttgaacaatacgaacgttctgaaggccgtcactctaccggtggtatggatgaactgtacaaa mCherry(Vn): prefix: A - suffix: ATCC - sequence: N + suffix: GGATCC + sequence: atggtttctaaaggtgaagaggataacatggcgatcatcaaagaatttatgcgcttcaaagttcacatggaaggttctgttaacggccacgaatttgaaattgaaggtgaaggcgaaggtcgtccatacgaaggtactcaaaccgcaaaactgaaagttaccaaaggtggtccactgccatttgcatgggatattctgtctccacagtttatgtacggtagcaaagcatacgttaaacacccagcagatattccagattacctgaaactgtcttttccggaaggtttcaaatgggaacgtgttatgaattttgaagatggtggtgttgttacggttacccaagattcttctctgcaagatggtgagtttatctacaaagttaaactgcgtggcaccaactttccatctgatggtccagttatgcagaaaaaaaccatgggttgggaagcatcttctgaacgtatgtacccagaagatggcgcactgaaaggtgaaattaaacaacgtctgaaacttaaagatggcggtcactacgatgcagaagttaaaaccacctacaaagcgaaaaaaccagttcaactgccaggtgcatacaacgttaacattaaactggatatcaccagccacaacgaagattacaccattgttgaacaatacgaacgtgcagaaggccgtcactctaccggtggtatggatgaactgtacaaa mKate-2(Vn): prefix: A - suffix: ATCC - sequence: N + suffix: GGATCC + sequence: atggtttctgaactgattaaagaaaacatgcacatgaaactgtacatggaaggtactgttaacaaccaccacttcaaatgtacctctgaaggtgaaggtaaaccatacgaaggtactcaaaccatgcgtattaaagcagttgaaggtggtccactgccatttgcatttgatattctggcaacctcttttatgtacggcagcaaaacctttatcaaccacactcaaggtatcccggattttttcaaacaaagctttccagaaggtttcacctgggaacgtgttaccacctacgaagatggtggtgttctgaccgcaactcaagatacctctctgcaagatggttgtctgatctacaacgttaaaatccgtggtgttaactttccatctaacggtccagttatgcagaaaaaaaccttaggttgggaagcatctaccgaaactctgtacccagcggatggtggtctggaaggtcgtgcagatatggcactgaaactggttggtggtggtcacctgatttgtaacctgaaaaccacctaccgttctaaaaaaccagcgaaaaatctgaaaatgccaggtgtttactacgttgatcgtcgtctggaacgtatcaaagaagcagataaagaaacctacgtggaacaacacgaagttgcagttgcacgttactgtgatctgccatctaaactgggtcaccgt # terminators -- make ctag versions as well B0010_terminator: prefix: ATCCTAA suffix: CGCT - sequence: N + sequence: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc B0015_terminator: prefix: ATCCTAA suffix: CGCT - sequence: N + sequence: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata B1002_terminator: prefix: ATCCTAA suffix: CGCT - sequence: N + sequence: cgcaaaaaaccccgcttcggcggggttttttcgc B1003_terminator: prefix: ATCCTAA suffix: CGCT - sequence: N + sequence: cgccaaaaaccccgcttcggcggggtttttccgc B1004_terminator: prefix: ATCCTAA suffix: CGCT - sequence: N + sequence: cgccgaaaaccccgcttcggcggggttttgccgc +B1005_terminator: + prefix: ATCCTAA + suffix: CGCT + sequence: cgccgcaaaccccgcttcggcggggtttcgccgc B1006_terminator: prefix: ATCCTAA suffix: CGCT - sequence: N + sequence: aaaaaaaaaccccgcccctgacagggcggggtttttttt B1007_terminator: prefix: ATCCTAA suffix: CGCT - sequence: N + sequence: cgcaaaaaaccccgcccctgacagggcggggttttttcgc B1009_terminator: prefix: ATCCTAA suffix: CGCT - sequence: N + sequence: cgccgaaaaccccgcccctgacagggcggggttttgccgc B1010_terminator: prefix: ATCCTAA suffix: CGCT - sequence: N + sequence: cgccgcaaaccccgcccctgacagggcggggtttcgccgc +Vnat_dummy_terminator: + prefix: ATCCTAA + suffix: CGCT + sequence: actcagttgtagtaacgagcggatagattcccagacccaccttcacgggcggtagcaggacctcaataataggattttcg +#ctag terminator +B0010_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc +B0015_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata +B1002_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: cgcaaaaaaccccgcttcggcggggttttttcgc +B1003_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: cgccaaaaaccccgcttcggcggggtttttccgc +B1004_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: cgccgaaaaccccgcttcggcggggttttgccgc +B1005_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: cgccgcaaaccccgcttcggcggggtttcgccgc +B1006_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: aaaaaaaaaccccgcccctgacagggcggggtttttttt +B1007_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: cgcaaaaaaccccgcccctgacagggcggggttttttcgc +B1009_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: cgccgaaaaccccgcccctgacagggcggggttttgccgc +B1010_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: cgccgcaaaccccgcccctgacagggcggggtttcgccgc +Vnat_dummy_terminator_ctag: + prefix: ATGT + suffix: CGCT + sequence: actcagttgtagtaacgagcggatagattcccagacccaccttcacgggcggtagcaggacctcaataataggattttcg # n and c tag # ntags: 3xFlag, 6xHis, Azurite, mTurqoise, sfGFP, mVenus, mScarlet-I, mCherry, mKate-2 3xFlag(Vn)_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: gattataaggatcatgatggtgattataaggatcatgatatcgactacaaagacgatgacgacaag 6xHis(Vn)_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: caccatcaccaccatcat Azurite(Vn)_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: atgtctaaaggtgaagaactgtttaccggtgttgttccaattctggttgaactggatggtgatgttaacggtcacaaattttctgtttctggtgaaggcgaaggtgatgcaacctacggtaaactgaccctgaaatttatctgtaccactggtaaactgccagttccatggccaactctggttaccactctgtctcacggtgttcaatgtttttctcgttacccagatcacatgaaacagcacgattttttcaaaagcgcaatgccagaaggttacgttcaagaacgtaccatcttcttcaaagatgacggtaactacaaaacccgtgcggaagtgaaatttgaaggtgataccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaggatggcaacattctgggtcacaaactggaatacaactttaactctcacaacatctacatcatggcggacaaacaaaaaaacggcatcaaagtgaacttcaagattcgccacaacatcgaagatggttctgttcaactggcagatcactaccaacaaaacaccccaattggtgatggtccagttctgctgccagataaccactacctgtctacccaatctgcactgtctaaagatccaaacgaaaaacgtgatcacatggtgctgctggaatttcgtaccgcagcaggtattacccacggtatggatgaactgtacaaa mTurquoise(Vn)_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: atggtttctaaaggtgaagaactgtttaccggtgttgttccaattctggttgaactggatggtgatgttaacggtcacaaattttctgtttctggtgaaggcgaaggtgatgcaacctacggtaaactgaccctgaaatttatctgtaccactggtaaactgccagttccatggccaactctggttaccactctgtcttggggtgttcaatgttttgcacgttacccagatcacatgaaacaacacgattttttcaaaagcgcaatgccagaaggttacgttcaagaacgtaccatcttcttcaaagatgacggtaactacaaaacccgtgcggaagtgaaatttgaaggtgataccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaggatggcaacattctgggtcacaaactggaatacaactactttagcgataacgtgtacatcaccgcagataaacaaaaaaacggtatcaaggcgaacttcaaaatccgtcacaacattgaagatggtggtgttcaactggcagatcactaccaacaaaacactccaattggtgatggtccagttctgctgccagataaccactacctgtctacccaatctaaactgtctaaagacccaaacgaaaaacgtgatcacatggttctgctggaatttgttaccgcagcaggtattaccttaggtatggatgaactgtacaaa sfGFP(Vn)_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: atgcgtaaaggtgaagaactgtttaccggtgttgttccaattctggttgaactggatggtgatgttaacggtcacaaattttctgttcgtggtgaaggcgaaggtgatgcaaccaacggtaaactgaccctgaaatttatctgtaccactggtaaactgccagttccatggccaactctggttaccactctgacctacggtgttcaatgttttgcacgttacccagatcacatgaaacaacacgattttttcaaaagcgcaatgccagaaggttacgttcaagaacgtaccatctcttttaaagatgacggcacctacaaaacccgtgcggaagttaaatttgaaggtgataccctggttaaccgcattgaactgaaaggcatcgattttaaagaagatggtaacatcctgggccacaaactggaatacaactttaactctcacaacgtgtacatcaccgcagacaaacaaaaaaacggtatcaaagcgaacttcaagatccgtcacaacgttgaagatggttctgttcaactggcagatcactaccaacaaaacaccccaattggtgatggtccagttctgctgccagataaccactacctgtctacccaaagcgttctgtctaaagatccaaacgaaaaacgtgatcacatggtgctgctggaatttgttaccgcagcaggtattacccacggtatggatgaactgtacaaagca mVenus(Vn)_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: atggtttctaaaggtgaagaactgtttaccggtgttgttccaattctggttgaactggatggtgatgttaacggtcacaaattttctgtttctggtgaaggcgaaggtgatgcaacctacggtaaactgaccctgaaactgatttgtaccactggtaaactgccagttccatggccaactctggttaccaccttaggttacggtctgcaatgttttgcacgttacccagatcacatgaaacaacacgattttttcaaaagcgcaatgccagaaggttacgttcaagaacgtaccatcttcttcaaagatgacggtaactacaaaacccgtgcggaagtgaaatttgaaggtgataccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaggatggcaacattctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgcagataaacaaaaaaacggcatcaaagcgaacttcaaaatccgtcacaacattgaagatggtggtgttcaactggcagatcactaccaacaaaacaccccaattggtgatggtccagttctgctgccagataaccactacctgtcttaccaatctaaactgagcaaagacccaaacgaaaaacgtgatcacatggttctgctggaatttgttaccgcagcaggtattaccttaggtatggatgaactgtacaaa mScarlet-I(Vn)_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: atggtttctaaaggtgaagcagtgatcaaagaatttatgcgcttcaaagttcacatggaaggttctatgaacggccacgaatttgaaattgaaggtgaaggcgaaggtcgtccatacgaaggtactcaaaccgcaaaactgaaagttaccaaaggtggtccactgccattttcttgggatattctgtctccacaatttatgtacggttctcgtgcatttatcaaacacccagcagatattccagactactacaaacaatcttttccggaaggtttcaaatgggaacgtgttatgaattttgaagatggtggtgcagttacggttacccaagatacctctctggaagatggtactctgatctacaaagttaaactgcgtggtactaactttccaccagatggtccagttatgcagaaaaaaaccatgggttgggaagcatctaccgaacgtctgtacccagaagatggcgttctgaaaggtgatatcaaaatggcactgcgtctgaaagatggcggtcgttacctggcagatttcaaaaccacctacaaagcgaaaaaaccagttcaaatgccaggtgcatacaacgttgatcgtaaactggatattaccagccacaacgaagattacaccgttgttgaacaatacgaacgttctgaaggccgtcactctaccggtggtatggatgaactgtacaaa mCherry_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: atggtttctaaaggtgaagaggataacatggcgatcatcaaagaatttatgcgcttcaaagttcacatggaaggttctgttaacggccacgaatttgaaattgaaggtgaaggcgaaggtcgtccatacgaaggtactcaaaccgcaaaactgaaagttaccaaaggtggtccactgccatttgcatgggatattctgtctccacagtttatgtacggtagcaaagcatacgttaaacacccagcagatattccagattacctgaaactgtcttttccggaaggtttcaaatgggaacgtgttatgaattttgaagatggtggtgttgttacggttacccaagattcttctctgcaagatggtgagtttatctacaaagttaaactgcgtggcaccaactttccatctgatggtccagttatgcagaaaaaaaccatgggttgggaagcatcttctgaacgtatgtacccagaagatggcgcactgaaaggtgaaattaaacaacgtctgaaacttaaagatggcggtcactacgatgcagaagttaaaaccacctacaaagcgaaaaaaccagttcaactgccaggtgcatacaacgttaacattaaactggatatcaccagccacaacgaagattacaccattgttgaacaatacgaacgtgcagaaggccgtcactctaccggtggtatggatgaactgtacaaa mKate-2_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: atggtttctgaactgattaaagaaaacatgcacatgaaactgtacatggaaggtactgttaacaaccaccacttcaaatgtacctctgaaggtgaaggtaaaccatacgaaggtactcaaaccatgcgtattaaagcagttgaaggtggtccactgccatttgcatttgatattctggcaacctcttttatgtacggcagcaaaacctttatcaaccacactcaaggtatcccggattttttcaaacaaagctttccagaaggtttcacctgggaacgtgttaccacctacgaagatggtggtgttctgaccgcaactcaagatacctctctgcaagatggttgtctgatctacaacgttaaaatccgtggtgttaactttccatctaacggtccagttatgcagaaaaaaaccttaggttgggaagcatctaccgaaactctgtacccagcggatggtggtctggaaggtcgtgcagatatggcactgaaactggttggtggtggtcacctgatttgtaacctgaaaaccacctaccgttctaaaaaaccagcgaaaaatctgaaaatgccaggtgtttactacgttgatcgtcgtctggaacgtatcaaagaagcagataaagaaacctacgtggaacaacacgaagttgcagttgcacgttactgtgatctgccatctaaactgggtcaccgt I11012_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: gcagcaaacgacgaaaactacgctgctgctgtt M0050_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: gctgctaacgacgaaaactacgctctggctgct M0051_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: gctgctaacgacgaaaactacaactacgctgacgcttct M0052_ctag: prefix: ATCC - suffix: ATGT - sequence: N + suffix: TAAATGT + sequence: gctgctaacgacgaaaactacgctgacgcttct