diff --git a/disperser/apiserver/disperse_blob_v2.go b/disperser/apiserver/disperse_blob_v2.go
index 39ed0b4c19..eb0a2155fa 100644
--- a/disperser/apiserver/disperse_blob_v2.go
+++ b/disperser/apiserver/disperse_blob_v2.go
@@ -29,7 +29,7 @@ func (s *DispersalServerV2) DisperseBlob(ctx context.Context, req *pb.DisperseBl
 	if onchainState == nil {
 		return nil, api.NewErrorInternal("onchain state is nil")
 	}
-	if err := s.validateDispersalRequest(req, onchainState); err != nil {
+	if err := s.validateDispersalRequest(ctx, req, onchainState); err != nil {
 		return nil, api.NewErrorInvalidArg(fmt.Sprintf("failed to validate the request: %v", err))
 	}
 
@@ -133,6 +133,7 @@ func (s *DispersalServerV2) checkPaymentMeter(ctx context.Context, req *pb.Dispe
 }
 
 func (s *DispersalServerV2) validateDispersalRequest(
+	ctx context.Context,
 	req *pb.DisperseBlobRequest,
 	onchainState *OnchainState) error {
 
@@ -220,5 +221,13 @@ func (s *DispersalServerV2) validateDispersalRequest(
 		return errors.New("invalid blob commitment")
 	}
 
+	blobKey, err := blobHeader.BlobKey()
+	if err != nil {
+		return fmt.Errorf("failed to get blob key: %w", err)
+	}
+	if s.blobStore.CheckBlobExists(ctx, blobKey) {
+		return fmt.Errorf("blob already exists: %s", blobKey.Hex())
+	}
+
 	return nil
 }
diff --git a/disperser/apiserver/server_v2_test.go b/disperser/apiserver/server_v2_test.go
index 8dfb3802ed..84f16e2f94 100644
--- a/disperser/apiserver/server_v2_test.go
+++ b/disperser/apiserver/server_v2_test.go
@@ -111,14 +111,45 @@ func TestV2DisperseBlob(t *testing.T) {
 	assert.Greater(t, blobMetadata.RequestedAt, uint64(now.UnixNano()))
 	assert.Equal(t, blobMetadata.RequestedAt, blobMetadata.UpdatedAt)
 
-	// Try dispersing the same blob; if payment is different, blob will be considered as a differernt blob
-	// payment will cause failure before commitment check
+	// Try dispersing the same blob; blob key check will fail if the blob is already stored
 	reply, err = c.DispersalServerV2.DisperseBlob(ctx, &pbv2.DisperseBlobRequest{
 		Blob:       data,
 		Signature:  sig,
 		BlobHeader: blobHeaderProto,
 	})
 	assert.Nil(t, reply)
+	assert.ErrorContains(t, err, "blob already exists")
+
+	data2 := make([]byte, 50)
+	_, err = rand.Read(data)
+	assert.NoError(t, err)
+
+	data2 = codec.ConvertByPaddingEmptyByte(data2)
+	commitments, err = prover.GetCommitmentsForPaddedLength(data2)
+	assert.NoError(t, err)
+	commitmentProto, err = commitments.ToProtobuf()
+	assert.NoError(t, err)
+	blobHeaderProto2 := &pbcommonv2.BlobHeader{
+		Version:       0,
+		QuorumNumbers: []uint32{0, 1},
+		Commitment:    commitmentProto,
+		PaymentHeader: &pbcommonv2.PaymentHeader{
+			AccountId:         accountID.Hex(),
+			Timestamp:         5,
+			CumulativePayment: big.NewInt(100).Bytes(),
+		},
+	}
+	blobHeader2, err := corev2.BlobHeaderFromProtobuf(blobHeaderProto2)
+	assert.NoError(t, err)
+	sig2, err := signer.SignBlobRequest(blobHeader2)
+	assert.NoError(t, err)
+
+	reply, err = c.DispersalServerV2.DisperseBlob(ctx, &pbv2.DisperseBlobRequest{
+		Blob:       data2,
+		Signature:  sig2,
+		BlobHeader: blobHeaderProto2,
+	})
+	assert.Nil(t, reply)
 	assert.ErrorContains(t, err, "payment already exists")
 }
 
diff --git a/disperser/common/v2/blobstore/s3_blob_store.go b/disperser/common/v2/blobstore/s3_blob_store.go
index 433af0a765..ef06fc0631 100644
--- a/disperser/common/v2/blobstore/s3_blob_store.go
+++ b/disperser/common/v2/blobstore/s3_blob_store.go
@@ -26,13 +26,12 @@ func NewBlobStore(s3BucketName string, s3Client s3.Client, logger logging.Logger
 
 // StoreBlob adds a blob to the blob store
 func (b *BlobStore) StoreBlob(ctx context.Context, key corev2.BlobKey, data []byte) error {
-	_, err := b.s3Client.HeadObject(ctx, b.bucketName, s3.ScopedBlobKey(key))
-	if err == nil {
+	if b.CheckBlobExists(ctx, key) {
 		b.logger.Warnf("blob already exists in bucket %s: %s", b.bucketName, key)
 		return common.ErrAlreadyExists
 	}
 
-	err = b.s3Client.UploadObject(ctx, b.bucketName, s3.ScopedBlobKey(key), data)
+	err := b.s3Client.UploadObject(ctx, b.bucketName, s3.ScopedBlobKey(key), data)
 	if err != nil {
 		b.logger.Errorf("failed to upload blob in bucket %s: %v", b.bucketName, err)
 		return err
@@ -40,6 +39,12 @@ func (b *BlobStore) StoreBlob(ctx context.Context, key corev2.BlobKey, data []by
 	return nil
 }
 
+// CheckBlobExists checks if a blob exists in the blob store
+func (b *BlobStore) CheckBlobExists(ctx context.Context, key corev2.BlobKey) bool {
+	_, err := b.s3Client.HeadObject(ctx, b.bucketName, s3.ScopedBlobKey(key))
+	return err == nil
+}
+
 // GetBlob retrieves a blob from the blob store
 func (b *BlobStore) GetBlob(ctx context.Context, key corev2.BlobKey) ([]byte, error) {
 	data, err := b.s3Client.DownloadObject(ctx, b.bucketName, s3.ScopedBlobKey(key))