diff --git a/.github/workflows/gh-ci.yaml b/.github/workflows/gh-ci.yaml
index 5240308..397a5a2 100644
--- a/.github/workflows/gh-ci.yaml
+++ b/.github/workflows/gh-ci.yaml
@@ -31,13 +31,13 @@ jobs:
         matrix:
           os: [ubuntu-latest, macOS-latest, windows-latest]
           mdanalysis-version: ["latest", "develop"]
-          python-version: ["3.10", "3.11", "3.12"]
+          python-version: ["3.10", "3.11", "3.12", "3.13"]
           exclude:
             # Entries here exclude particular combinations of the matrix
             # Edit or remove as particular combinations come into or out of date
             # Below we exclude runs with the latest release and Python 3.12
             - mdanalysis-version: "latest"
-              python-version: "3.12"
+              python-version: "3.13"
 
     steps:
     - uses: actions/checkout@v4
@@ -56,7 +56,6 @@ jobs:
         python-version: ${{ matrix.python-version }}
         environment-file: devtools/conda-envs/test_env.yaml
         add-pip-as-python-dependency: true
-        architecture: x64
 
         miniforge-version: "latest"
         use-mamba: true
diff --git a/pyproject.toml b/pyproject.toml
index e0936ce..9c3cf67 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -16,7 +16,7 @@ maintainers = [
     {name = "Your name (or your organization/company/team)", email = "fiona@mdanalysis.org"},
 ]
 readme = "README.md"
-requires-python = ">=3.9"
+requires-python = ">=3.10"
 dependencies = [
     "MDAnalysis>=2.0.0",
 ]