From 7a390217e72718f9a13c60025b9877a550bca7d0 Mon Sep 17 00:00:00 2001 From: Xiaoyan Qian Date: Mon, 3 Apr 2017 11:16:38 +0200 Subject: [PATCH] Update readme.txt --- readme.txt | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/readme.txt b/readme.txt index 4264289..6f72312 100644 --- a/readme.txt +++ b/readme.txt @@ -8,7 +8,8 @@ Install clustalW, add to path Install Python 2, add to path Add folder Scripts in Python installation folder to path Open command window, type: pip install numpy -From ftp.ncbi.nlm.nih.gov download refseq mouse and human mRNA sequences and decompress to a desired location +From ftp.ncbi.nlm.nih.gov download refseq mouse and human mRNA sequences and umcompress to a desired location +Download the repository and umcompress to a desired location Modify config.py file to specify where you have saved mRNA fasta files Modify config.py to specify maximum number of parallel threads for MSA (multiple sequence alignment) and blast search Double click probedesign.py to run