This workshop, conducted by the Monash Bioinformatics Platform, will cover how to extend the Seurat 3K PBMC tutorial with third party tools SingleR and Harmony
To render the Rmd file to HTML and R use:
Rscript build.R
Current order of play and nominated instructors (TBC)
| Time | Section | Lead Instructor | Timing Estimates |
|---|---|---|---|
| 9:30 - 10:00 | Workshop overview | Adele | 30 min |
| 10:00 - 10:10 | Setup | 10 min | |
| 10:10 - 10:30 | Seurat Data Objects | Adele | 20 min |
| 10:30 - 11:00 | Data QC | Adele | 30 min |
| 11:00 - 11:15 | Break | 15 min | |
| 11:15 - 12:00 | Normalisation | Paul | 45 min |
| 12:00 - 1:00 | Lunch break | 60 min | |
| 1:00 - 1:45 | Dimensionality Reduction | Paul | 45 min |
| 1:45 - 2:30 | Clustering | Paul/Laura | 45 min |
| 2:30 - 2:45 | Break | 15 min | |
| 2:45 - 3:30 | Cluster Identification | Adele | 45 min |
| 3:30 - 4:00 | Single Cell further (SingleR annotation content) | Adele | 30 min |
| 4:00 - 4:30 | Harmony, Q&A, etc | Paul/Open | 30 min |