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CDS translation is broken with bacterial trans_table=11 #86

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lfalquet opened this issue Oct 2, 2024 · 3 comments
Open

CDS translation is broken with bacterial trans_table=11 #86

lfalquet opened this issue Oct 2, 2024 · 3 comments
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@lfalquet
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lfalquet commented Oct 2, 2024

Describe the bug
The translation into protein creates an issue when the translation table is set to "11" (bacterial code).

When the start codon is NOT a canonical "ATG" (e.g., GTG), the translation misses the quotes around the sequence.

FT /transl_table=11
FT /translation=GTGIRAGGCGIGCFPGKGEMEFRILGSVQIHDERFGVRIVPAGAKQ
FT RALLGALLVRAGQAVPAECLVEELWGGHPPVNAANALQAHVARLRRLLPAPGPGGPRHV
FT WLRTSPLGYTLSLGPAATDAQRFHRLVGQGRELAATAPGRAVEVLREGLALWRGPALQG
FT SGQGPICSAEAALLEESRLRALEVLYDVCLRADRCAEITGELEELVAVHPLRERFHEQL
FT MTALHRCGRRAEALSTYDRARRRLARDLGIGPGQVLSRRREAILRSCEPAADSADRAGA
FT GTAGQGGDELGVLREELVRLRGHVELLRRQQRELSEQVARLTAHHPCGP

It works correctly for the other CDS where the start codon is the canonical "ATG"

FT /transl_table=11
FT /translation="MEIKVLGALNAEFEGISVVPSAGKPRQILALLALYPGRVVPVPTL
FT MEEIWGTDLPQSSMTTLQTYILQLRRLLGTAMGPDVPGSAKDVLATRYGGYLLQIPAEA
FT VDAFTYERLVTEGRQAYEDGEDERAATVLRRALDLWDGPALVDVRVGPVLEIEAMRLEQ
FT SRLVARERRIDADLRLGRHVELIAELTDLIARHPQHEGLHSQAMVALYRSGRQAAALDV
FT YRRLRQRLIDELGVEPSPQLQRLHQAMLAVDPRLDIVAGPRRTSTFDLYAA"

Thanks for correcting this bug.

Best greetings,
Laurent

@lfalquet
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lfalquet commented Oct 2, 2024

It also put "GTG" instead of "M", I have just noticed...

@Juke34 Juke34 added the bug label Oct 3, 2024
@Juke34
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Juke34 commented Oct 3, 2024

Thank you for reporting this bug.

Have in mind that submitting translated CDS is not mandatory. EBI will make and add the translation.

@lfalquet
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lfalquet commented Oct 4, 2024 via email

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