diff --git a/neofox/__init__.py b/neofox/__init__.py
index 5212506a..c6321016 100755
--- a/neofox/__init__.py
+++ b/neofox/__init__.py
@@ -16,7 +16,7 @@
 #
 # You should have received a copy of the GNU General Public License
 # along with this program. If not, see <http://www.gnu.org/licenses/>.#
-VERSION = "0.6.3"
+VERSION = "0.6.4"
 
 
 REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER"
diff --git a/requirements.txt b/requirements.txt
index 9e719883..0a64df35 100755
--- a/requirements.txt
+++ b/requirements.txt
@@ -1,14 +1,13 @@
-biopython~=1.76
+biopython==1.76
 mock~=4.0.3
 pandas>=1.1.5
 numpy>=1.21
 scipy>=1.5.4
-pickle-mixin~=1.0.2
 scikit-learn~=0.22.1
 logzero>=1.5.0
 python-dotenv~=0.12.0
 betterproto~=1.2.5
-pysam~=0.16.0.1
+pysam~=0.19.1
 dask[complete]>=2021.10.0
 distributed>=2021.10.0
 faker~=6.6.2