MSA problem in R Studio

[oliver7423]

Hi! I'm working for my midterms and I need answers ASAP

I downloaded sequences from NCBI (Complete Record > Fasta) and I installed necessary packages. By the time I reached using msa, the error appears. I don't know what to do anymore. Help! This is the codes by the way.

AlignNeeded = TRUE # The program reads fasta file and aligns it
if (AlignNeeded) {

file <- readDNAStringSet(fname)#for reading multiple DNA sequences from msa package
file
}

cb<- msa(file) # multiple sequence alignment from msa package
cb # aligned the 120 sequences in the file

use default substitution matrix
Error in convertAlnRows(result$msa, type) : There is an invalid aln file!

AFTER READING A SOLUTION HERE, I TRIED IT OUT, HERE'S THE PROBLEM

cb<- msa(file) # multiple sequence alignment from msa package
use default substitution matrix
Error in convertAlnRows(result$msa, type) : There is an invalid aln file!
library(Biostrings)
file_path <- "sequence-5.fasta"
cb <- msa(file_path, filetype = "sequence-5.fasta", type = "DNA")
Error in msaFun(inputSeqs = inputSeqs, cluster = cluster, gapOpening = gapOpening, :
The following parameters are not known
(or have been specifiedmore often than once):
filetype

PLEASE HELP ME

[oliver7423]

im good. i just needed an aligned file.