This dataset contains live-cell time-lapse microscopy of pyroptosis in SH-SY5Y cells. The dataset was generated by Saguaro BioSciences and University of Montreal's imaging platform.
The dataset was generated by imaging SH-SY5Y cells that were treated with pyroptosis-inducing agents. The cells were imaged every 3 hours for 48 hours using a 20x objective on a Yokogawa CQ1 cell voyager spinning disk confocal microscope. The images were acquired with the following parameters:
- 20x objective - NA 0.80
- 96 wells on a 384-well plate
- 5 fields of view (FOV) per well
- 1 z-slice per FOV
- 4 channels
The images were acquired in 4 channels:
| Channel # | Channel Name | Excitation (nm) | Emission (nm) | Dye | Exposure (ms) |
|---|---|---|---|---|---|
| 1 | GSDMD | 488 | 525/50 | GFP | 50 |
| 2 | CL488 | 488 | 685/40 | Chromalive 488 | 400 |
| 3 | CL561 | 561 | 617/73 | Chromalive 561 | 700 |
| 4 | DNA | 405 | 447/60 | Hoechst 33342 | 600 |
| 5 | Brightfield | 200 |
The wave 1 plate map can be viewed in the Wave1_data/platemap_readme.md.
This wave 1 data is preliminary and generated to identify the best conditions for imaging pyroptosis in SH-SY5Y cells. The data has the following naming convention:
YYYYMMDDTHHMMSS_W####F####T####Z###C#.tifYYYYMMDDTHHMMSS: Date and time of image acquisitionW####: Well numberF####: Field of view numberT####: Timepoint numberZ###: Z-slice numberC#: Channel number
These raw data are preprocessed into single well, single field of view, single timepoint, single z-slice images directories with all channels. This allows for each FOV to be processed independently.