diff --git a/sample-apps/python-manual-instrumentation-sample-app/requirements.txt b/sample-apps/python-manual-instrumentation-sample-app/requirements.txt
index 2e79195f..cff047f0 100644
--- a/sample-apps/python-manual-instrumentation-sample-app/requirements.txt
+++ b/sample-apps/python-manual-instrumentation-sample-app/requirements.txt
@@ -1,4 +1,4 @@
-boto3==1.34.19
+boto3==1.34.25
 Flask==2.3.3
 opentelemetry-distro==0.43b0
 opentelemetry-exporter-otlp==1.22.0