diff --git a/hatch_build.py b/hatch_build.py
index 837c47f01..2bfa8d8ce 100644
--- a/hatch_build.py
+++ b/hatch_build.py
@@ -44,7 +44,16 @@ def initialize(self, version, build_data):
         )
         try:
             subprocess.check_call([npm_path, "install"], cwd="web-frontend")
-            subprocess.check_call([npm_path, "run", "build"], cwd="web-frontend")
+            subprocess.check_call(
+                [
+                    npm_path,
+                    "run",
+                    "build",
+                    "--",
+                    f"--base={os.environ.get('TILED_BUILD_PUBLIC_PATH', '/ui/')}",
+                ],
+                cwd="web-frontend",
+            )
             if Path(artifact_path).exists():
                 shutil.rmtree(artifact_path)
             shutil.copytree("web-frontend/dist", artifact_path)
diff --git a/tiled/commandline/_serve.py b/tiled/commandline/_serve.py
index 39e9d2372..01ca96782 100644
--- a/tiled/commandline/_serve.py
+++ b/tiled/commandline/_serve.py
@@ -612,6 +612,10 @@ def serve_config(
 
     # This config was already validated when it was parsed. Do not re-validate.
     logger.info(f"Using configuration from {Path(config_path).absolute()}")
+
+    if root_path := uvicorn_kwargs.get("root_path", ""):
+        parsed_config["root_path"] = root_path
+
     web_app = build_app_from_config(
         parsed_config, source_filepath=config_path, scalable=scalable
     )
diff --git a/tiled/config.py b/tiled/config.py
index 22d76dcec..0f568a06e 100644
--- a/tiled/config.py
+++ b/tiled/config.py
@@ -183,6 +183,8 @@ def construct_build_app_kwargs(
             root_tree = MapAdapter(root_mapping, access_policy=root_access_policy)
             root_tree.include_routers.extend(include_routers)
         server_settings = {}
+        if root_path := config.get("root_path", ""):
+            server_settings["root_path"] = root_path
         server_settings["allow_origins"] = config.get("allow_origins")
         server_settings["object_cache"] = config.get("object_cache", {})
         server_settings["response_bytesize_limit"] = config.get(
diff --git a/tiled/server/app.py b/tiled/server/app.py
index 0c3ac7a71..8a05854c7 100644
--- a/tiled/server/app.py
+++ b/tiled/server/app.py
@@ -266,6 +266,8 @@ async def index(
             # comments. But they are served as JSON because that is easy to deal with
             # on the client side.
             ui_settings = yaml.safe_load(Path(TILED_UI_SETTINGS).read_text())
+            if root_path := server_settings.get("root_path", ""):
+                ui_settings["api_url"] = f"{root_path}{ui_settings['api_url']}"
 
             @app.get("/tiled-ui-settings")
             async def tiled_ui_settings():
diff --git a/web-frontend/src/settings.ts b/web-frontend/src/settings.ts
index 218ab9a1e..3800061c1 100644
--- a/web-frontend/src/settings.ts
+++ b/web-frontend/src/settings.ts
@@ -1,5 +1,7 @@
-const tiledUISettingsURL = "/tiled-ui-settings";
-// TODO Enable a different URL to be chosen at build time?
+const basename = import.meta.env.BASE_URL;
+
+const tiledUISettingsURL = basename.split('/').slice(0, -2).join('/') + '/tiled-ui-settings';
+// Alternate idea
 // const tiledUISettingsURL = import.meta.env.TILED_UI_SETTINGS || "/tiled-ui-settings";
 
 interface Column {