Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Option to create (strand-specific) coverage bigwigs #14

Open
SamBryce-Smith opened this issue Jan 14, 2021 · 1 comment
Open

Option to create (strand-specific) coverage bigwigs #14

SamBryce-Smith opened this issue Jan 14, 2021 · 1 comment
Labels
enhancement New feature or request low priority

Comments

@SamBryce-Smith
Copy link
Member

Following on from this week's concerns, it could be a good idea to have the option to generate plus & minus bigwigs for each BAM file, so can visualise strand-specific coverage over regions.

I can't see too many benefits on the splicing side, but for APA this could be particularly handy. Especially for regions where there are overlapping genes on opposite strands, it's not always super clear from the IGV BAM coverage profile whether it comes from one gene or the other.

Bigwigs are supposed to be smaller than BAMs, so could make IGV browsing more lightweight too...

Deeptools seems like a good suite to use for this, but will look into it on the side. If we do end up implementing this should definitely be optional

@SamBryce-Smith SamBryce-Smith added the enhancement New feature or request label Jan 14, 2021
@SamBryce-Smith
Copy link
Member Author

The conda env env/align.yaml has deeptools installed but haven't done anything with it. Hooking up should be straightforward (even with multiqc, some of their QC modules look handy)

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
enhancement New feature or request low priority
Projects
None yet
Development

No branches or pull requests

1 participant