diff --git a/dev-packages/node-integration-tests/suites/express/multiple-routers/complex-router/test.ts b/dev-packages/node-integration-tests/suites/express/multiple-routers/complex-router/test.ts
index 606120248e35..fe065d0dc550 100644
--- a/dev-packages/node-integration-tests/suites/express/multiple-routers/complex-router/test.ts
+++ b/dev-packages/node-integration-tests/suites/express/multiple-routers/complex-router/test.ts
@@ -1,31 +1,17 @@
-import { conditionalTest } from '../../../../utils';
 import { cleanupChildProcesses, createRunner } from '../../../../utils/runner';
 
 afterAll(() => {
   cleanupChildProcesses();
 });
 
-// Before Node 16, parametrization is not working properly here
-conditionalTest({ min: 16 })('complex-router', () => {
+describe('complex-router', () => {
   test('should construct correct url with multiple parameterized routers, when param is also contain in middle layer route and express used multiple middlewares with route', done => {
-    // parse node.js major version
-    const [major = 0] = process.versions.node.split('.').map(Number);
-    // Split test result base on major node version because regex d flag is support from node 16+
-
-    const EXPECTED_TRANSACTION =
-      major >= 16
-        ? {
-            transaction: 'GET /api/api/v1/sub-router/users/:userId/posts/:postId',
-            transaction_info: {
-              source: 'route',
-            },
-          }
-        : {
-            transaction: 'GET /api/api/v1/sub-router/users/123/posts/:postId',
-            transaction_info: {
-              source: 'route',
-            },
-          };
+    const EXPECTED_TRANSACTION = {
+      transaction: 'GET /api/api/v1/sub-router/users/:userId/posts/:postId',
+      transaction_info: {
+        source: 'route',
+      },
+    };
 
     createRunner(__dirname, 'server.ts')
       .ignore('event')
@@ -35,23 +21,12 @@ conditionalTest({ min: 16 })('complex-router', () => {
   });
 
   test('should construct correct url with multiple parameterized routers, when param is also contain in middle layer route and express used multiple middlewares with route and original url has query params', done => {
-    // parse node.js major version
-    const [major = 0] = process.versions.node.split('.').map(Number);
-    // Split test result base on major node version because regex d flag is support from node 16+
-    const EXPECTED_TRANSACTION =
-      major >= 16
-        ? {
-            transaction: 'GET /api/api/v1/sub-router/users/:userId/posts/:postId',
-            transaction_info: {
-              source: 'route',
-            },
-          }
-        : {
-            transaction: 'GET /api/api/v1/sub-router/users/123/posts/:postId',
-            transaction_info: {
-              source: 'route',
-            },
-          };
+    const EXPECTED_TRANSACTION = {
+      transaction: 'GET /api/api/v1/sub-router/users/:userId/posts/:postId',
+      transaction_info: {
+        source: 'route',
+      },
+    };
 
     createRunner(__dirname, 'server.ts')
       .ignore('event')
@@ -61,23 +36,12 @@ conditionalTest({ min: 16 })('complex-router', () => {
   });
 
   test('should construct correct url with multiple parameterized routers, when param is also contain in middle layer route and express used multiple middlewares with route and original url ends with trailing slash and has query params', done => {
-    // parse node.js major version
-    const [major = 0] = process.versions.node.split('.').map(Number);
-    // Split test result base on major node version because regex d flag is support from node 16+
-    const EXPECTED_TRANSACTION =
-      major >= 16
-        ? {
-            transaction: 'GET /api/api/v1/sub-router/users/:userId/posts/:postId',
-            transaction_info: {
-              source: 'route',
-            },
-          }
-        : {
-            transaction: 'GET /api/api/v1/sub-router/users/123/posts/:postId',
-            transaction_info: {
-              source: 'route',
-            },
-          };
+    const EXPECTED_TRANSACTION = {
+      transaction: 'GET /api/api/v1/sub-router/users/:userId/posts/:postId',
+      transaction_info: {
+        source: 'route',
+      },
+    };
 
     createRunner(__dirname, 'server.ts')
       .ignore('event')
diff --git a/dev-packages/node-integration-tests/suites/express/multiple-routers/middle-layer-parameterized/test.ts b/dev-packages/node-integration-tests/suites/express/multiple-routers/middle-layer-parameterized/test.ts
index 4a6ae304af14..52a6ce154684 100644
--- a/dev-packages/node-integration-tests/suites/express/multiple-routers/middle-layer-parameterized/test.ts
+++ b/dev-packages/node-integration-tests/suites/express/multiple-routers/middle-layer-parameterized/test.ts
@@ -1,4 +1,3 @@
-import { conditionalTest } from '../../../../utils';
 import { cleanupChildProcesses, createRunner } from '../../../../utils/runner';
 
 afterAll(() => {
@@ -6,25 +5,14 @@ afterAll(() => {
 });
 
 // Before Node 16, parametrization is not working properly here
-conditionalTest({ min: 16 })('middle-layer-parameterized', () => {
+describe('middle-layer-parameterized', () => {
   test('should construct correct url with multiple parameterized routers, when param is also contain in middle layer route', done => {
-    // parse node.js major version
-    const [major = 0] = process.versions.node.split('.').map(Number);
-    // Split test result base on major node version because regex d flag is support from node 16+
-    const EXPECTED_TRANSACTION =
-      major >= 16
-        ? {
-            transaction: 'GET /api/v1/users/:userId/posts/:postId',
-            transaction_info: {
-              source: 'route',
-            },
-          }
-        : {
-            transaction: 'GET /api/v1/users/123/posts/:postId',
-            transaction_info: {
-              source: 'route',
-            },
-          };
+    const EXPECTED_TRANSACTION = {
+      transaction: 'GET /api/v1/users/:userId/posts/:postId',
+      transaction_info: {
+        source: 'route',
+      },
+    };
 
     createRunner(__dirname, 'server.ts')
       .ignore('event')
diff --git a/packages/node/package.json b/packages/node/package.json
index 8145cf18a7d6..52559d93ed27 100644
--- a/packages/node/package.json
+++ b/packages/node/package.json
@@ -72,7 +72,7 @@
     "@opentelemetry/instrumentation-amqplib": "^0.42.0",
     "@opentelemetry/instrumentation-connect": "0.39.0",
     "@opentelemetry/instrumentation-dataloader": "0.12.0",
-    "@opentelemetry/instrumentation-express": "0.42.0",
+    "@opentelemetry/instrumentation-express": "0.43.0",
     "@opentelemetry/instrumentation-fastify": "0.39.0",
     "@opentelemetry/instrumentation-fs": "0.15.0",
     "@opentelemetry/instrumentation-generic-pool": "0.39.0",
diff --git a/yarn.lock b/yarn.lock
index 5ab8ce9b9952..2a31856c98dd 100644
--- a/yarn.lock
+++ b/yarn.lock
@@ -7326,10 +7326,10 @@
   dependencies:
     "@opentelemetry/instrumentation" "^0.53.0"
 
-"@opentelemetry/instrumentation-express@0.42.0":
-  version "0.42.0"
-  resolved "https://registry.yarnpkg.com/@opentelemetry/instrumentation-express/-/instrumentation-express-0.42.0.tgz#279f195aa66baee2b98623a16666c6229c8e7564"
-  integrity sha512-YNcy7ZfGnLsVEqGXQPT+S0G1AE46N21ORY7i7yUQyfhGAL4RBjnZUqefMI0NwqIl6nGbr1IpF0rZGoN8Q7x12Q==
+"@opentelemetry/instrumentation-express@0.43.0":
+  version "0.43.0"
+  resolved "https://registry.yarnpkg.com/@opentelemetry/instrumentation-express/-/instrumentation-express-0.43.0.tgz#35ff5bcf40b816d9a9159d5f7814ed7e5d83f69b"
+  integrity sha512-bxTIlzn9qPXJgrhz8/Do5Q3jIlqfpoJrSUtVGqH+90eM1v2PkPHc+SdE+zSqe4q9Y1UQJosmZ4N4bm7Zj/++MA==
   dependencies:
     "@opentelemetry/core" "^1.8.0"
     "@opentelemetry/instrumentation" "^0.53.0"