diff --git a/google-cloud-spanner/src/test/java/com/google/cloud/spanner/RetryOnDifferentGrpcChannelMockServerTest.java b/google-cloud-spanner/src/test/java/com/google/cloud/spanner/RetryOnDifferentGrpcChannelMockServerTest.java
index 267c6077ad..c436947336 100644
--- a/google-cloud-spanner/src/test/java/com/google/cloud/spanner/RetryOnDifferentGrpcChannelMockServerTest.java
+++ b/google-cloud-spanner/src/test/java/com/google/cloud/spanner/RetryOnDifferentGrpcChannelMockServerTest.java
@@ -59,6 +59,7 @@
 import org.junit.Test;
 import org.junit.runner.RunWith;
 import org.junit.runners.JUnit4;
+import static org.junit.Assume.assumeFalse;
 
 @RunWith(JUnit4.class)
 public class RetryOnDifferentGrpcChannelMockServerTest extends AbstractMockServerTest {
@@ -133,6 +134,9 @@ public void testReadWriteTransaction_retriesOnNewChannel() {
     AtomicInteger attempts = new AtomicInteger();
 
     try (Spanner spanner = builder.build().getService()) {
+      assumeFalse(
+          "RetryOnDifferentGrpcChannel handler is not implemented for read-write with multiplexed sessions",
+          isMultiplexedSessionsEnabledForRW(spanner));
       DatabaseClient client = spanner.getDatabaseClient(DatabaseId.of("p", "i", "d"));
       client
           .readWriteTransaction()
@@ -168,6 +172,9 @@ public void testReadWriteTransaction_stopsRetrying() {
         SimulatedExecutionTime.ofStickyException(Status.DEADLINE_EXCEEDED.asRuntimeException()));
 
     try (Spanner spanner = builder.build().getService()) {
+      assumeFalse(
+          "RetryOnDifferentGrpcChannel handler is not implemented for read-write with multiplexed sessions",
+          isMultiplexedSessionsEnabledForRW(spanner));
       DatabaseClient client = spanner.getDatabaseClient(DatabaseId.of("p", "i", "d"));
       SpannerException exception =
           assertThrows(
@@ -211,6 +218,9 @@ public void testDenyListedChannelIsCleared() {
         SimulatedExecutionTime.ofStickyException(Status.DEADLINE_EXCEEDED.asRuntimeException()));
 
     try (Spanner spanner = builder.build().getService()) {
+      assumeFalse(
+          "RetryOnDifferentGrpcChannel handler is not implemented for read-write with multiplexed sessions",
+          isMultiplexedSessionsEnabledForRW(spanner));
       DatabaseClient client = spanner.getDatabaseClient(DatabaseId.of("p", "i", "d"));
 
       // Retry until all channels have been deny-listed.
@@ -339,6 +349,9 @@ public void testReadWriteTransaction_withGrpcContextDeadline_doesNotRetry() {
         SimulatedExecutionTime.ofMinimumAndRandomTime(500, 500));
 
     try (Spanner spanner = builder.build().getService()) {
+      assumeFalse(
+          "RetryOnDifferentGrpcChannel handler is not implemented for read-write with multiplexed sessions",
+          isMultiplexedSessionsEnabledForRW(spanner));
       DatabaseClient client = spanner.getDatabaseClient(DatabaseId.of("p", "i", "d"));
       ScheduledExecutorService service = Executors.newScheduledThreadPool(1);
       Context context =
@@ -365,4 +378,11 @@ public void testReadWriteTransaction_withGrpcContextDeadline_doesNotRetry() {
     // up.
     assertEquals(1, mockSpanner.countRequestsOfType(BeginTransactionRequest.class));
   }
+
+  private boolean isMultiplexedSessionsEnabledForRW(Spanner spanner) {
+    if (spanner.getOptions() == null || spanner.getOptions().getSessionPoolOptions() == null) {
+      return false;
+    }
+    return spanner.getOptions().getSessionPoolOptions().getUseMultiplexedSessionForRW();
+  }
 }