diff --git a/test/e2e/nodenetworkpolicy_test.go b/test/e2e/nodenetworkpolicy_test.go
index 5564fd37329..dd5b287fdf9 100644
--- a/test/e2e/nodenetworkpolicy_test.go
+++ b/test/e2e/nodenetworkpolicy_test.go
@@ -29,7 +29,7 @@ import (
 
 const labelNodeHostname = "kubernetes.io/hostname"
 
-func initializeAntreaNodeNetworkPolicy(t *testing.T, data *TestData, toHostNetworkPod bool) {
+func initializeAntreaNodeNetworkPolicy(t *testing.T, data *TestData, testNodeToNode bool) {
 	p80 = 80
 	p81 = 81
 	p8080 = 8080
@@ -39,22 +39,42 @@ func initializeAntreaNodeNetworkPolicy(t *testing.T, data *TestData, toHostNetwo
 	podsPerNamespace = []string{"a"}
 	suffix := randName("")
 	namespaces = make(map[string]TestNamespaceMeta)
-	for _, ns := range []string{"x", "y", "z"} {
-		namespaces[ns] = TestNamespaceMeta{
-			Name: ns + "-" + suffix,
-		}
-	}
 	nodes = make(map[string]string)
-	nodes["x"] = controlPlaneNodeName()
-	nodes["y"] = workerNodeName(1)
 	hostNetworks := make(map[string]bool)
+
+	// Deploy a hostNetwork Pod in Namespace with prefix "x-" on a Node.
+	nodes["x"] = nodeName(0)
 	hostNetworks["x"] = true
-	if toHostNetworkPod {
+	namespaces["x"] = TestNamespaceMeta{
+		Name: "x-" + suffix,
+	}
+
+	if testNodeToNode {
+		// To test NodeNetworkPolicy between Nodes, deploy another hostNetwork Pod in Namespace prefixed with "y-" on
+		// another Node. Pod in Namespace with prefix "z-" is not needed.
+		nodes["y"] = nodeName(1)
 		hostNetworks["y"] = true
+		namespaces["y"] = TestNamespaceMeta{
+			Name: "y-" + suffix,
+		}
 	} else {
+		// To test NodeNetworkPolicy between Nodes and Pods, deploy another two non-hostNetwork Pods in Namespaces
+		// prefixed with "y-" and "z-", respectively, on another Node.
+		// It is important to note that we avoid deploying non-hostNetwork Pods and hostNetwork Pods on the same Node
+		// for this test. If so, after all test Pods are created, the UDP probing from a non-hostNetwork Pod to the
+		// hostNetwork Pod deployed on the same Node will get a failure. The reason is that the reply packets use the
+		// local Antrea gateway IP as source IP, instead of the local Node IP, which is the destination IP of the request
+		// packets.
+		nodes["y"] = nodeName(1)
 		hostNetworks["y"] = false
-		nodes["z"] = workerNodeName(1)
+		namespaces["y"] = TestNamespaceMeta{
+			Name: "y-" + suffix,
+		}
+		nodes["z"] = nodeName(1)
 		hostNetworks["z"] = false
+		namespaces["z"] = TestNamespaceMeta{
+			Name: "z-" + suffix,
+		}
 	}
 	allPods = []Pod{}
 
@@ -89,6 +109,7 @@ func TestAntreaNodeNetworkPolicy(t *testing.T) {
 	}
 	defer teardownTest(t, data)
 
+	// Test NodeNetworkPolicy between Nodes.
 	initializeAntreaNodeNetworkPolicy(t, data, true)
 
 	t.Run("Case=ACNPAllowNoDefaultIsolationTCP", func(t *testing.T) { testNodeACNPAllowNoDefaultIsolation(t, ProtocolTCP) })
@@ -115,6 +136,7 @@ func TestAntreaNodeNetworkPolicy(t *testing.T) {
 
 	k8sUtils.Cleanup(namespaces)
 
+	// Test NodeNetworkPolicy between Nodes and Pods.
 	initializeAntreaNodeNetworkPolicy(t, data, false)
 
 	t.Run("Case=ACNPNamespaceIsolation", func(t *testing.T) { testNodeACNPNamespaceIsolation(t) })