SBE4_image_GFPRFP.ipynb

{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": 71,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T17:06:28.226335Z",
     "start_time": "2021-05-18T17:06:28.221534Z"
    }
   },
   "outputs": [],
   "source": [
    "#!pip3 install plotly"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 82,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T17:09:27.743840Z",
     "start_time": "2021-05-18T17:09:27.736837Z"
    }
   },
   "outputs": [
    {
     "data": {
      "text/html": [
       "        <script type=\"text/javascript\">\n",
       "        window.PlotlyConfig = {MathJaxConfig: 'local'};\n",
       "        if (window.MathJax) {MathJax.Hub.Config({SVG: {font: \"STIX-Web\"}});}\n",
       "        if (typeof require !== 'undefined') {\n",
       "        require.undef(\"plotly\");\n",
       "        requirejs.config({\n",
       "            paths: {\n",
       "                'plotly': ['https://cdn.plot.ly/plotly-latest.min']\n",
       "            }\n",
       "        });\n",
       "        require(['plotly'], function(Plotly) {\n",
       "            window._Plotly = Plotly;\n",
       "        });\n",
       "        }\n",
       "        </script>\n",
       "        "
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "import pandas\n",
    "import plotly.express as px\n",
    "import plotly.graph_objects as go\n",
    "import plotly.figure_factory as ff\n",
    "from plotly.offline import download_plotlyjs, init_notebook_mode, iplot\n",
    "init_notebook_mode(connected=True)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 143,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T17:41:51.354295Z",
     "start_time": "2021-05-18T17:41:51.347407Z"
    }
   },
   "outputs": [],
   "source": [
    "### raw filename r04c11f04p01-ch2sk30fk1fl1.tiff\n",
    "ch1=\"GFP\"\n",
    "ch2=\"RFP\"\n",
    "construct_names = [\"construct SBE4\"]\n",
    "treatment_names = [\"DMSO\",\"Gherlin 75nM\", \"Gherlin 150nM\",\"Gherlin 300nM\",\"TGFbeta 2ng/mL\", \"TGFbeta 5ng/mL\", \"TGFbeta 10ng/mL\"]\n",
    "removeoutliers=True"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 144,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T17:41:52.067532Z",
     "start_time": "2021-05-18T17:41:52.020547Z"
    }
   },
   "outputs": [],
   "source": [
    "# def colinforlookup(row):\n",
    "#     colname = row[\"col\"]\n",
    "#     if colname==\"02\":\n",
    "#         return \"construct SBE4\"\n",
    "#     if colname==\"03\":\n",
    "#         return \"SBE4 construct\"\n",
    "#     if colname==\"04\":\n",
    "#         return \"SBE4 construct\"\n",
    "#     if colname==\"05\":\n",
    "#         return \"SBE4 construct\"\n",
    "#     if colname==\"06\":\n",
    "#         return \"SBE4 construct\"\n",
    "    \n",
    "def rowinforlookup(row):\n",
    "    rowname = row[\"row\"]\n",
    "    if rowname==\"r01\":\n",
    "        return \"DMSO\"\n",
    "    if rowname==\"r02\":\n",
    "        return \"Gherlin 75nM\"\n",
    "    if rowname==\"r03\":\n",
    "        return \"Gherlin 150nM\"\n",
    "    if rowname==\"r04\":\n",
    "        return \"Gherlin 300nM\"\n",
    "    \n",
    "def channellookup(row):\n",
    "    microscopechannel = row[\"colorcode\"]\n",
    "    if microscopechannel.startswith(\"ch1\"):\n",
    "        return ch1\n",
    "    if microscopechannel.startswith(\"ch2\"):\n",
    "        return ch2\n",
    "\n",
    "# def fixtime(time):\n",
    "#     if time.find('1fk1fl1-1') == 0:\n",
    "#         return '1fk1fl1'\n",
    "#     elif time.find('10fk1fl1-1') == 0:\n",
    "#         return '10fk1fl1'\n",
    "#     else:\n",
    "#         return time"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 167,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T18:08:26.670702Z",
     "start_time": "2021-05-18T18:08:26.651893Z"
    }
   },
   "outputs": [],
   "source": [
    "def findoutliers(df):\n",
    "    df1=df[df[\"color\"]==ch1]\n",
    "    df2=df[df[\"color\"]==ch2]\n",
    "    y = df1['MEDIAN']\n",
    "    removed_outliers = y.between(y.quantile(.1), y.quantile(.90))\n",
    "    df1 = df1[removed_outliers]\n",
    "    y2 = df2['MEDIAN']\n",
    "    removed_outliers = y2.between(y2.quantile(.1), y2.quantile(.90))\n",
    "    df2 = df2[removed_outliers]\n",
    "    return df1, df2"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 168,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T18:08:27.656351Z",
     "start_time": "2021-05-18T18:08:27.494228Z"
    }
   },
   "outputs": [],
   "source": [
    "def processrawdata(filename=\"/Users/jewe1055/experiments/exp64/scripts/SBE4_ImageJ.csv\"):\n",
    "    ### raw filename r04c11f04p01-ch2sk30fk1fl1.tiff\n",
    "    df = pandas.read_csv(filename)\n",
    "    df[['filename','imagename']] = df.LABEL.str.split(\".\",expand=True,)\n",
    "    df[['platepos','microscopeinfo']] = df[\"filename\"].str.split(\"-\",expand=True,n=1)\n",
    "    df[[\"colorcode\", \"time\"]]=df[\"microscopeinfo\"].str.split(\"sk\",expand=True,n=1)\n",
    "    df[\"color\"]=df.apply(lambda row : channellookup(row), axis = 1)\n",
    "    df[[\"rowcol\", \"frame\"]]=df[\"platepos\"].str.split(\"f\",expand=True,n=1)\n",
    "    df[[\"row\", \"col\"]]=df[\"rowcol\"].str.split(\"c\",expand=True,n=1)\n",
    "    df[\"condition_name\"] = df.apply(lambda row : rowinforlookup(row), axis = 1)\n",
    "    df[\"construct_name\"] = df.apply(lambda row : colinforlookup(row), axis = 1)\n",
    "    df['time']=df.apply(lambda row : fixtime(row['time']), axis = 1)\n",
    "    df = df[[\"LABEL\", 'AREA', 'MEAN', 'INTDEN', 'MEDIAN', 'RAWINTDEN', \"row\", \"col\", \"frame\", \"color\", \"time\", \"imagename\", \"condition_name\", \"construct_name\"]]\n",
    "    if removeoutliers==True:\n",
    "        df1,df2 = findoutliers(df)\n",
    "    else:\n",
    "        df1=df[df[\"color\"]==ch1]\n",
    "        df2=df[df[\"color\"]==ch2]\n",
    "    #throws away anything we don't have RFP and GFP\n",
    "    mdf = df1.merge(df2, on=[\"row\", \"col\", \"frame\",\"imagename\", \"AREA\", \"time\", \"condition_name\", \"construct_name\"], suffixes=(\"_\"+ch1, \"_\"+ch2))\n",
    "    print('mdf data shape ',df.shape)\n",
    "    #df1 and df2 should be half of samples then df\n",
    "    print('ch1 data shape ',ch1, df1.shape)\n",
    "    print('ch2 data shape ',ch2, df2.shape)\n",
    "    mdf['ratio'] = mdf[\"MEDIAN_\"+ch1]/mdf[\"MEDIAN_\"+ch2]\n",
    "    mdf_plusmissing = df1.merge(df2, on=[\"row\", \"col\", \"frame\",\"imagename\", \"AREA\", \"time\", \"condition_name\", \"construct_name\"], suffixes=(\"_\"+ch1, \"_\"+ch2), how=\"outer\")\n",
    "    print('mdf+missing data shape ', mdf_plusmissing.shape)\n",
    "    return mdf\n"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 169,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T18:08:28.534832Z",
     "start_time": "2021-05-18T18:08:28.170400Z"
    }
   },
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "mdf data shape  (1954, 14)\n",
      "ch1 data shape  GFP (801, 14)\n",
      "ch2 data shape  RFP (763, 14)\n",
      "mdf+missing data shape  (913, 20)\n"
     ]
    }
   ],
   "source": [
    "#throws away anything we don't have RFP and GFP\n",
    "mdf = processrawdata()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 170,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T18:08:29.042320Z",
     "start_time": "2021-05-18T18:08:28.857435Z"
    }
   },
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>LABEL_GFP</th>\n",
       "      <th>AREA</th>\n",
       "      <th>MEAN_GFP</th>\n",
       "      <th>INTDEN_GFP</th>\n",
       "      <th>MEDIAN_GFP</th>\n",
       "      <th>RAWINTDEN_GFP</th>\n",
       "      <th>row</th>\n",
       "      <th>col</th>\n",
       "      <th>frame</th>\n",
       "      <th>color_GFP</th>\n",
       "      <th>...</th>\n",
       "      <th>imagename</th>\n",
       "      <th>condition_name</th>\n",
       "      <th>construct_name</th>\n",
       "      <th>LABEL_RFP</th>\n",
       "      <th>MEAN_RFP</th>\n",
       "      <th>INTDEN_RFP</th>\n",
       "      <th>MEDIAN_RFP</th>\n",
       "      <th>RAWINTDEN_RFP</th>\n",
       "      <th>color_RFP</th>\n",
       "      <th>ratio</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>r01c02f01p01-ch1sk1fk1fl1.tiff:0004-0081</td>\n",
       "      <td>0.000064</td>\n",
       "      <td>553.831</td>\n",
       "      <td>0.035</td>\n",
       "      <td>553</td>\n",
       "      <td>85290</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0004-0081</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk1fk1fl1-1.tiff:0004-0081</td>\n",
       "      <td>489.636</td>\n",
       "      <td>0.031</td>\n",
       "      <td>486</td>\n",
       "      <td>75404</td>\n",
       "      <td>RFP</td>\n",
       "      <td>1.137860</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>r01c02f01p01-ch1sk1fk1fl1.tiff:0011-0346</td>\n",
       "      <td>0.000155</td>\n",
       "      <td>545.472</td>\n",
       "      <td>0.085</td>\n",
       "      <td>545</td>\n",
       "      <td>203461</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0011-0346</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk1fk1fl1-1.tiff:0011-0346</td>\n",
       "      <td>681.627</td>\n",
       "      <td>0.106</td>\n",
       "      <td>685</td>\n",
       "      <td>254247</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.795620</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>r01c02f01p01-ch1sk1fk1fl1.tiff:0012-0360</td>\n",
       "      <td>0.000170</td>\n",
       "      <td>464.282</td>\n",
       "      <td>0.079</td>\n",
       "      <td>453</td>\n",
       "      <td>189427</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0012-0360</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk1fk1fl1-1.tiff:0012-0360</td>\n",
       "      <td>618.995</td>\n",
       "      <td>0.105</td>\n",
       "      <td>622</td>\n",
       "      <td>252550</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.728296</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>r01c02f01p01-ch1sk1fk1fl1.tiff:0015-0406</td>\n",
       "      <td>0.000340</td>\n",
       "      <td>468.973</td>\n",
       "      <td>0.160</td>\n",
       "      <td>471</td>\n",
       "      <td>383620</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0015-0406</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk1fk1fl1-1.tiff:0015-0406</td>\n",
       "      <td>555.685</td>\n",
       "      <td>0.189</td>\n",
       "      <td>556</td>\n",
       "      <td>454550</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.847122</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>r01c02f01p01-ch1sk1fk1fl1.tiff:0017-0550</td>\n",
       "      <td>0.000369</td>\n",
       "      <td>548.068</td>\n",
       "      <td>0.202</td>\n",
       "      <td>553</td>\n",
       "      <td>485588</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0017-0550</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk1fk1fl1-1.tiff:0017-0550</td>\n",
       "      <td>575.968</td>\n",
       "      <td>0.212</td>\n",
       "      <td>576</td>\n",
       "      <td>510308</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.960069</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>r01c02f01p01-ch1sk1fk1fl1.tiff:0018-0568</td>\n",
       "      <td>0.000175</td>\n",
       "      <td>485.052</td>\n",
       "      <td>0.085</td>\n",
       "      <td>488</td>\n",
       "      <td>204207</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0018-0568</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk1fk1fl1-1.tiff:0018-0568</td>\n",
       "      <td>514.487</td>\n",
       "      <td>0.090</td>\n",
       "      <td>515</td>\n",
       "      <td>216599</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.947573</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>r01c02f01p01-ch1sk1fk1fl1.tiff:0024-0589</td>\n",
       "      <td>0.000133</td>\n",
       "      <td>473.363</td>\n",
       "      <td>0.063</td>\n",
       "      <td>473</td>\n",
       "      <td>151476</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0024-0589</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk1fk1fl1-1.tiff:0024-0589</td>\n",
       "      <td>486.413</td>\n",
       "      <td>0.065</td>\n",
       "      <td>484</td>\n",
       "      <td>155652</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.977273</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>r01c02f01p01-ch1sk1fk1fl1.tiff:0033-0636</td>\n",
       "      <td>0.000149</td>\n",
       "      <td>457.824</td>\n",
       "      <td>0.068</td>\n",
       "      <td>450</td>\n",
       "      <td>163901</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0033-0636</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk1fk1fl1-1.tiff:0033-0636</td>\n",
       "      <td>583.173</td>\n",
       "      <td>0.087</td>\n",
       "      <td>573</td>\n",
       "      <td>208776</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.785340</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>r01c02f01p01-ch1sk1fk1fl1.tiff:0035-1027</td>\n",
       "      <td>0.002000</td>\n",
       "      <td>592.269</td>\n",
       "      <td>1.228</td>\n",
       "      <td>581</td>\n",
       "      <td>2952460</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0035-1027</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk1fk1fl1-1.tiff:0035-1027</td>\n",
       "      <td>669.683</td>\n",
       "      <td>1.389</td>\n",
       "      <td>640</td>\n",
       "      <td>3338371</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.907813</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0004-0081</td>\n",
       "      <td>0.000064</td>\n",
       "      <td>542.825</td>\n",
       "      <td>0.035</td>\n",
       "      <td>545</td>\n",
       "      <td>83595</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0004-0081</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0004-0081</td>\n",
       "      <td>462.838</td>\n",
       "      <td>0.030</td>\n",
       "      <td>466</td>\n",
       "      <td>71277</td>\n",
       "      <td>RFP</td>\n",
       "      <td>1.169528</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0005-0264</td>\n",
       "      <td>0.000240</td>\n",
       "      <td>559.554</td>\n",
       "      <td>0.134</td>\n",
       "      <td>581</td>\n",
       "      <td>322303</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0005-0264</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0005-0264</td>\n",
       "      <td>472.014</td>\n",
       "      <td>0.113</td>\n",
       "      <td>477</td>\n",
       "      <td>271880</td>\n",
       "      <td>RFP</td>\n",
       "      <td>1.218029</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0011-0346</td>\n",
       "      <td>0.000155</td>\n",
       "      <td>572.660</td>\n",
       "      <td>0.089</td>\n",
       "      <td>570</td>\n",
       "      <td>213602</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0011-0346</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0011-0346</td>\n",
       "      <td>683.544</td>\n",
       "      <td>0.106</td>\n",
       "      <td>676</td>\n",
       "      <td>254962</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.843195</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0015-0406</td>\n",
       "      <td>0.000340</td>\n",
       "      <td>401.738</td>\n",
       "      <td>0.137</td>\n",
       "      <td>429</td>\n",
       "      <td>328622</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0015-0406</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0015-0406</td>\n",
       "      <td>478.061</td>\n",
       "      <td>0.163</td>\n",
       "      <td>512</td>\n",
       "      <td>391054</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.837891</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0017-0550</td>\n",
       "      <td>0.000369</td>\n",
       "      <td>571.778</td>\n",
       "      <td>0.211</td>\n",
       "      <td>580</td>\n",
       "      <td>506595</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0017-0550</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0017-0550</td>\n",
       "      <td>613.533</td>\n",
       "      <td>0.226</td>\n",
       "      <td>627</td>\n",
       "      <td>543590</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.925040</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>14</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0018-0568</td>\n",
       "      <td>0.000175</td>\n",
       "      <td>405.767</td>\n",
       "      <td>0.071</td>\n",
       "      <td>422</td>\n",
       "      <td>170828</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0018-0568</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0018-0568</td>\n",
       "      <td>435.774</td>\n",
       "      <td>0.076</td>\n",
       "      <td>438</td>\n",
       "      <td>183461</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.963470</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>15</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0024-0589</td>\n",
       "      <td>0.000133</td>\n",
       "      <td>512.597</td>\n",
       "      <td>0.068</td>\n",
       "      <td>520</td>\n",
       "      <td>164031</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0024-0589</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0024-0589</td>\n",
       "      <td>539.966</td>\n",
       "      <td>0.072</td>\n",
       "      <td>543</td>\n",
       "      <td>172789</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.957643</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>16</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0030-0632</td>\n",
       "      <td>0.000879</td>\n",
       "      <td>587.348</td>\n",
       "      <td>0.516</td>\n",
       "      <td>543</td>\n",
       "      <td>1240478</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0030-0632</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0030-0632</td>\n",
       "      <td>668.534</td>\n",
       "      <td>0.587</td>\n",
       "      <td>688</td>\n",
       "      <td>1411944</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.789244</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>17</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0033-0636</td>\n",
       "      <td>0.000149</td>\n",
       "      <td>455.757</td>\n",
       "      <td>0.068</td>\n",
       "      <td>464</td>\n",
       "      <td>163161</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0033-0636</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0033-0636</td>\n",
       "      <td>637.827</td>\n",
       "      <td>0.095</td>\n",
       "      <td>665</td>\n",
       "      <td>228342</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.697744</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>18</th>\n",
       "      <td>r01c02f01p01-ch1sk2fk1fl1.tiff:0035-1027</td>\n",
       "      <td>0.002000</td>\n",
       "      <td>527.892</td>\n",
       "      <td>1.095</td>\n",
       "      <td>524</td>\n",
       "      <td>2631542</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0035-1027</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk2fk1fl1.tiff:0035-1027</td>\n",
       "      <td>647.740</td>\n",
       "      <td>1.343</td>\n",
       "      <td>624</td>\n",
       "      <td>3228983</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.839744</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>19</th>\n",
       "      <td>r01c02f01p01-ch1sk30fk1fl1.tiff:0004-0081</td>\n",
       "      <td>0.000064</td>\n",
       "      <td>475.091</td>\n",
       "      <td>0.030</td>\n",
       "      <td>481</td>\n",
       "      <td>73164</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0004-0081</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk30fk1fl1.tiff:0004-0081</td>\n",
       "      <td>411.422</td>\n",
       "      <td>0.026</td>\n",
       "      <td>412</td>\n",
       "      <td>63359</td>\n",
       "      <td>RFP</td>\n",
       "      <td>1.167476</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>20</th>\n",
       "      <td>r01c02f01p01-ch1sk30fk1fl1.tiff:0010-0343</td>\n",
       "      <td>0.000153</td>\n",
       "      <td>563.832</td>\n",
       "      <td>0.086</td>\n",
       "      <td>568</td>\n",
       "      <td>207490</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0010-0343</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk30fk1fl1.tiff:0010-0343</td>\n",
       "      <td>609.812</td>\n",
       "      <td>0.093</td>\n",
       "      <td>636</td>\n",
       "      <td>224411</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.893082</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>21</th>\n",
       "      <td>r01c02f01p01-ch1sk30fk1fl1.tiff:0015-0406</td>\n",
       "      <td>0.000340</td>\n",
       "      <td>465.597</td>\n",
       "      <td>0.158</td>\n",
       "      <td>465</td>\n",
       "      <td>380858</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0015-0406</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk30fk1fl1.tiff:0015-0406</td>\n",
       "      <td>500.249</td>\n",
       "      <td>0.170</td>\n",
       "      <td>514</td>\n",
       "      <td>409204</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.904669</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>22</th>\n",
       "      <td>r01c02f01p01-ch1sk30fk1fl1.tiff:0017-0550</td>\n",
       "      <td>0.000369</td>\n",
       "      <td>460.093</td>\n",
       "      <td>0.170</td>\n",
       "      <td>447</td>\n",
       "      <td>407642</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0017-0550</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk30fk1fl1.tiff:0017-0550</td>\n",
       "      <td>498.806</td>\n",
       "      <td>0.184</td>\n",
       "      <td>493</td>\n",
       "      <td>441942</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.906694</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>23</th>\n",
       "      <td>r01c02f01p01-ch1sk30fk1fl1.tiff:0018-0568</td>\n",
       "      <td>0.000175</td>\n",
       "      <td>525.599</td>\n",
       "      <td>0.092</td>\n",
       "      <td>564</td>\n",
       "      <td>221277</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0018-0568</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk30fk1fl1.tiff:0018-0568</td>\n",
       "      <td>641.865</td>\n",
       "      <td>0.112</td>\n",
       "      <td>694</td>\n",
       "      <td>270225</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.812680</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>24</th>\n",
       "      <td>r01c02f01p01-ch1sk30fk1fl1.tiff:0024-0589</td>\n",
       "      <td>0.000133</td>\n",
       "      <td>454.525</td>\n",
       "      <td>0.061</td>\n",
       "      <td>467</td>\n",
       "      <td>145448</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0024-0589</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk30fk1fl1.tiff:0024-0589</td>\n",
       "      <td>527.966</td>\n",
       "      <td>0.070</td>\n",
       "      <td>534</td>\n",
       "      <td>168949</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.874532</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>25</th>\n",
       "      <td>r01c02f01p01-ch1sk30fk1fl1.tiff:0030-0632</td>\n",
       "      <td>0.000879</td>\n",
       "      <td>554.246</td>\n",
       "      <td>0.487</td>\n",
       "      <td>508</td>\n",
       "      <td>1170568</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0030-0632</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk30fk1fl1.tiff:0030-0632</td>\n",
       "      <td>557.796</td>\n",
       "      <td>0.490</td>\n",
       "      <td>555</td>\n",
       "      <td>1178066</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.915315</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>26</th>\n",
       "      <td>r01c02f01p01-ch1sk30fk1fl1.tiff:0033-0636</td>\n",
       "      <td>0.000149</td>\n",
       "      <td>518.760</td>\n",
       "      <td>0.077</td>\n",
       "      <td>528</td>\n",
       "      <td>185716</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0033-0636</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk30fk1fl1.tiff:0033-0636</td>\n",
       "      <td>697.824</td>\n",
       "      <td>0.104</td>\n",
       "      <td>713</td>\n",
       "      <td>249821</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.740533</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>27</th>\n",
       "      <td>r01c02f01p01-ch1sk30fk1fl1.tiff:0035-1027</td>\n",
       "      <td>0.002000</td>\n",
       "      <td>488.006</td>\n",
       "      <td>1.012</td>\n",
       "      <td>487</td>\n",
       "      <td>2432708</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0035-1027</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk30fk1fl1.tiff:0035-1027</td>\n",
       "      <td>612.195</td>\n",
       "      <td>1.270</td>\n",
       "      <td>627</td>\n",
       "      <td>3051794</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.776715</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>28</th>\n",
       "      <td>r01c02f01p01-ch1sk31fk1fl1.tiff:0010-0343</td>\n",
       "      <td>0.000153</td>\n",
       "      <td>552.562</td>\n",
       "      <td>0.085</td>\n",
       "      <td>551</td>\n",
       "      <td>203343</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0010-0343</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk31fk1fl1.tiff:0010-0343</td>\n",
       "      <td>601.315</td>\n",
       "      <td>0.092</td>\n",
       "      <td>593</td>\n",
       "      <td>221284</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.929174</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>29</th>\n",
       "      <td>r01c02f01p01-ch1sk31fk1fl1.tiff:0015-0406</td>\n",
       "      <td>0.000340</td>\n",
       "      <td>466.748</td>\n",
       "      <td>0.159</td>\n",
       "      <td>466</td>\n",
       "      <td>381800</td>\n",
       "      <td>r01</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0015-0406</td>\n",
       "      <td>DMSO</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r01c02f01p01-ch2sk31fk1fl1.tiff:0015-0406</td>\n",
       "      <td>504.518</td>\n",
       "      <td>0.172</td>\n",
       "      <td>512</td>\n",
       "      <td>412696</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.910156</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>...</th>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>638</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0012-0476</td>\n",
       "      <td>0.000552</td>\n",
       "      <td>365.396</td>\n",
       "      <td>0.202</td>\n",
       "      <td>366</td>\n",
       "      <td>484881</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0012-0476</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0012-0476</td>\n",
       "      <td>412.456</td>\n",
       "      <td>0.228</td>\n",
       "      <td>422</td>\n",
       "      <td>547329</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.867299</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>639</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0014-0511</td>\n",
       "      <td>0.000439</td>\n",
       "      <td>445.350</td>\n",
       "      <td>0.196</td>\n",
       "      <td>471</td>\n",
       "      <td>470290</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0014-0511</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0014-0511</td>\n",
       "      <td>484.253</td>\n",
       "      <td>0.213</td>\n",
       "      <td>516</td>\n",
       "      <td>511371</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.912791</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>640</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0019-0669</td>\n",
       "      <td>0.000382</td>\n",
       "      <td>493.125</td>\n",
       "      <td>0.188</td>\n",
       "      <td>509</td>\n",
       "      <td>452196</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0019-0669</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0019-0669</td>\n",
       "      <td>533.277</td>\n",
       "      <td>0.203</td>\n",
       "      <td>554</td>\n",
       "      <td>489015</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.918773</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>641</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0020-0684</td>\n",
       "      <td>0.000740</td>\n",
       "      <td>491.595</td>\n",
       "      <td>0.364</td>\n",
       "      <td>503</td>\n",
       "      <td>874548</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0020-0684</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0020-0684</td>\n",
       "      <td>524.469</td>\n",
       "      <td>0.388</td>\n",
       "      <td>516</td>\n",
       "      <td>933031</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.974806</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>642</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0021-0685</td>\n",
       "      <td>0.000900</td>\n",
       "      <td>518.010</td>\n",
       "      <td>0.466</td>\n",
       "      <td>510</td>\n",
       "      <td>1120974</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0021-0685</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0021-0685</td>\n",
       "      <td>559.112</td>\n",
       "      <td>0.503</td>\n",
       "      <td>536</td>\n",
       "      <td>1209918</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.951493</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>643</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0022-0686</td>\n",
       "      <td>0.000635</td>\n",
       "      <td>462.233</td>\n",
       "      <td>0.293</td>\n",
       "      <td>478</td>\n",
       "      <td>704905</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0022-0686</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0022-0686</td>\n",
       "      <td>617.284</td>\n",
       "      <td>0.392</td>\n",
       "      <td>634</td>\n",
       "      <td>941358</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.753943</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>644</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0030-0882</td>\n",
       "      <td>0.000298</td>\n",
       "      <td>375.736</td>\n",
       "      <td>0.112</td>\n",
       "      <td>379</td>\n",
       "      <td>269403</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0030-0882</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0030-0882</td>\n",
       "      <td>455.165</td>\n",
       "      <td>0.136</td>\n",
       "      <td>480</td>\n",
       "      <td>326353</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.789583</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>645</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0031-0879</td>\n",
       "      <td>0.000338</td>\n",
       "      <td>436.345</td>\n",
       "      <td>0.147</td>\n",
       "      <td>432</td>\n",
       "      <td>354312</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0031-0879</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0031-0879</td>\n",
       "      <td>586.560</td>\n",
       "      <td>0.198</td>\n",
       "      <td>599</td>\n",
       "      <td>476287</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.721202</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>646</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0033-0891</td>\n",
       "      <td>0.000340</td>\n",
       "      <td>366.053</td>\n",
       "      <td>0.125</td>\n",
       "      <td>364</td>\n",
       "      <td>299431</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0033-0891</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0033-0891</td>\n",
       "      <td>474.610</td>\n",
       "      <td>0.162</td>\n",
       "      <td>521</td>\n",
       "      <td>388231</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.698656</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>647</th>\n",
       "      <td>r02c02f01p01-ch1sk17fk1fl1.tiff:0003-0069</td>\n",
       "      <td>0.000393</td>\n",
       "      <td>354.342</td>\n",
       "      <td>0.139</td>\n",
       "      <td>373</td>\n",
       "      <td>334499</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0003-0069</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk17fk1fl1.tiff:0003-0069</td>\n",
       "      <td>477.731</td>\n",
       "      <td>0.188</td>\n",
       "      <td>511</td>\n",
       "      <td>450978</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.729941</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>648</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0008-0384</td>\n",
       "      <td>0.000774</td>\n",
       "      <td>434.758</td>\n",
       "      <td>0.337</td>\n",
       "      <td>446</td>\n",
       "      <td>809085</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0008-0384</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0008-0384</td>\n",
       "      <td>563.090</td>\n",
       "      <td>0.436</td>\n",
       "      <td>578</td>\n",
       "      <td>1047911</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.771626</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>649</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0014-0511</td>\n",
       "      <td>0.000439</td>\n",
       "      <td>441.911</td>\n",
       "      <td>0.194</td>\n",
       "      <td>470</td>\n",
       "      <td>466658</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0014-0511</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0014-0511</td>\n",
       "      <td>465.562</td>\n",
       "      <td>0.205</td>\n",
       "      <td>492</td>\n",
       "      <td>491633</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.955285</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>650</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0019-0669</td>\n",
       "      <td>0.000382</td>\n",
       "      <td>497.399</td>\n",
       "      <td>0.190</td>\n",
       "      <td>509</td>\n",
       "      <td>456115</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0019-0669</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0019-0669</td>\n",
       "      <td>525.501</td>\n",
       "      <td>0.200</td>\n",
       "      <td>547</td>\n",
       "      <td>481884</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.930530</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>651</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0020-0684</td>\n",
       "      <td>0.000740</td>\n",
       "      <td>477.103</td>\n",
       "      <td>0.353</td>\n",
       "      <td>479</td>\n",
       "      <td>848766</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0020-0684</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0020-0684</td>\n",
       "      <td>489.070</td>\n",
       "      <td>0.362</td>\n",
       "      <td>461</td>\n",
       "      <td>870055</td>\n",
       "      <td>RFP</td>\n",
       "      <td>1.039046</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>652</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0021-0685</td>\n",
       "      <td>0.000900</td>\n",
       "      <td>486.860</td>\n",
       "      <td>0.438</td>\n",
       "      <td>482</td>\n",
       "      <td>1053566</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0021-0685</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0021-0685</td>\n",
       "      <td>499.098</td>\n",
       "      <td>0.449</td>\n",
       "      <td>477</td>\n",
       "      <td>1080049</td>\n",
       "      <td>RFP</td>\n",
       "      <td>1.010482</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>653</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0022-0686</td>\n",
       "      <td>0.000635</td>\n",
       "      <td>451.412</td>\n",
       "      <td>0.286</td>\n",
       "      <td>463</td>\n",
       "      <td>688404</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0022-0686</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0022-0686</td>\n",
       "      <td>573.012</td>\n",
       "      <td>0.364</td>\n",
       "      <td>578</td>\n",
       "      <td>873843</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.801038</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>654</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0030-0882</td>\n",
       "      <td>0.000298</td>\n",
       "      <td>376.321</td>\n",
       "      <td>0.112</td>\n",
       "      <td>385</td>\n",
       "      <td>269822</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0030-0882</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0030-0882</td>\n",
       "      <td>456.767</td>\n",
       "      <td>0.136</td>\n",
       "      <td>485</td>\n",
       "      <td>327502</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.793814</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>655</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0031-0879</td>\n",
       "      <td>0.000338</td>\n",
       "      <td>431.844</td>\n",
       "      <td>0.146</td>\n",
       "      <td>425</td>\n",
       "      <td>350657</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0031-0879</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0031-0879</td>\n",
       "      <td>564.272</td>\n",
       "      <td>0.191</td>\n",
       "      <td>575</td>\n",
       "      <td>458189</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.739130</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>656</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0033-0891</td>\n",
       "      <td>0.000340</td>\n",
       "      <td>352.996</td>\n",
       "      <td>0.120</td>\n",
       "      <td>367</td>\n",
       "      <td>288751</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0033-0891</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0033-0891</td>\n",
       "      <td>434.361</td>\n",
       "      <td>0.148</td>\n",
       "      <td>439</td>\n",
       "      <td>355307</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.835991</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>657</th>\n",
       "      <td>r02c02f01p01-ch1sk18fk1fl1.tiff:0003-0069</td>\n",
       "      <td>0.000393</td>\n",
       "      <td>363.964</td>\n",
       "      <td>0.143</td>\n",
       "      <td>382</td>\n",
       "      <td>343582</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0003-0069</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk18fk1fl1.tiff:0003-0069</td>\n",
       "      <td>473.531</td>\n",
       "      <td>0.186</td>\n",
       "      <td>496</td>\n",
       "      <td>447013</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.770161</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>658</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0008-0384</td>\n",
       "      <td>0.000774</td>\n",
       "      <td>442.850</td>\n",
       "      <td>0.343</td>\n",
       "      <td>457</td>\n",
       "      <td>824143</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0008-0384</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0008-0384</td>\n",
       "      <td>611.382</td>\n",
       "      <td>0.473</td>\n",
       "      <td>644</td>\n",
       "      <td>1137782</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.709627</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>659</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0012-0476</td>\n",
       "      <td>0.000552</td>\n",
       "      <td>371.795</td>\n",
       "      <td>0.205</td>\n",
       "      <td>370</td>\n",
       "      <td>493372</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0012-0476</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0012-0476</td>\n",
       "      <td>437.625</td>\n",
       "      <td>0.242</td>\n",
       "      <td>451</td>\n",
       "      <td>580728</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.820399</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>660</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0014-0511</td>\n",
       "      <td>0.000439</td>\n",
       "      <td>461.214</td>\n",
       "      <td>0.203</td>\n",
       "      <td>488</td>\n",
       "      <td>487042</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0014-0511</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0014-0511</td>\n",
       "      <td>500.688</td>\n",
       "      <td>0.220</td>\n",
       "      <td>542</td>\n",
       "      <td>528727</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.900369</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>661</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0019-0669</td>\n",
       "      <td>0.000382</td>\n",
       "      <td>498.410</td>\n",
       "      <td>0.190</td>\n",
       "      <td>509</td>\n",
       "      <td>457042</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0019-0669</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0019-0669</td>\n",
       "      <td>553.228</td>\n",
       "      <td>0.211</td>\n",
       "      <td>573</td>\n",
       "      <td>507310</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.888307</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>662</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0020-0684</td>\n",
       "      <td>0.000740</td>\n",
       "      <td>499.659</td>\n",
       "      <td>0.370</td>\n",
       "      <td>517</td>\n",
       "      <td>888893</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0020-0684</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0020-0684</td>\n",
       "      <td>558.211</td>\n",
       "      <td>0.413</td>\n",
       "      <td>565</td>\n",
       "      <td>993057</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.915044</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>663</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0021-0685</td>\n",
       "      <td>0.000900</td>\n",
       "      <td>518.022</td>\n",
       "      <td>0.466</td>\n",
       "      <td>516</td>\n",
       "      <td>1121000</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0021-0685</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0021-0685</td>\n",
       "      <td>583.122</td>\n",
       "      <td>0.525</td>\n",
       "      <td>565</td>\n",
       "      <td>1261877</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.913274</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>664</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0022-0686</td>\n",
       "      <td>0.000635</td>\n",
       "      <td>465.980</td>\n",
       "      <td>0.296</td>\n",
       "      <td>475</td>\n",
       "      <td>710620</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0022-0686</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0022-0686</td>\n",
       "      <td>641.907</td>\n",
       "      <td>0.407</td>\n",
       "      <td>667</td>\n",
       "      <td>978908</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.712144</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>665</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0030-0882</td>\n",
       "      <td>0.000298</td>\n",
       "      <td>370.755</td>\n",
       "      <td>0.111</td>\n",
       "      <td>374</td>\n",
       "      <td>265831</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0030-0882</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0030-0882</td>\n",
       "      <td>464.840</td>\n",
       "      <td>0.139</td>\n",
       "      <td>481</td>\n",
       "      <td>333290</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.777547</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>666</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0031-0879</td>\n",
       "      <td>0.000338</td>\n",
       "      <td>438.303</td>\n",
       "      <td>0.148</td>\n",
       "      <td>435</td>\n",
       "      <td>355902</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0031-0879</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0031-0879</td>\n",
       "      <td>611.695</td>\n",
       "      <td>0.207</td>\n",
       "      <td>617</td>\n",
       "      <td>496696</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.705024</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>667</th>\n",
       "      <td>r02c02f01p01-ch1sk19fk1fl1.tiff:0003-0069</td>\n",
       "      <td>0.000393</td>\n",
       "      <td>371.397</td>\n",
       "      <td>0.146</td>\n",
       "      <td>386</td>\n",
       "      <td>350599</td>\n",
       "      <td>r02</td>\n",
       "      <td>02</td>\n",
       "      <td>01p01</td>\n",
       "      <td>GFP</td>\n",
       "      <td>...</td>\n",
       "      <td>tiff:0003-0069</td>\n",
       "      <td>Gherlin 75nM</td>\n",
       "      <td>SBE4 construct</td>\n",
       "      <td>r02c02f01p01-ch2sk19fk1fl1.tiff:0003-0069</td>\n",
       "      <td>515.318</td>\n",
       "      <td>0.202</td>\n",
       "      <td>546</td>\n",
       "      <td>486460</td>\n",
       "      <td>RFP</td>\n",
       "      <td>0.706960</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>668 rows × 21 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "                                     LABEL_GFP      AREA  MEAN_GFP  \\\n",
       "0     r01c02f01p01-ch1sk1fk1fl1.tiff:0004-0081  0.000064   553.831   \n",
       "1     r01c02f01p01-ch1sk1fk1fl1.tiff:0011-0346  0.000155   545.472   \n",
       "2     r01c02f01p01-ch1sk1fk1fl1.tiff:0012-0360  0.000170   464.282   \n",
       "3     r01c02f01p01-ch1sk1fk1fl1.tiff:0015-0406  0.000340   468.973   \n",
       "4     r01c02f01p01-ch1sk1fk1fl1.tiff:0017-0550  0.000369   548.068   \n",
       "5     r01c02f01p01-ch1sk1fk1fl1.tiff:0018-0568  0.000175   485.052   \n",
       "6     r01c02f01p01-ch1sk1fk1fl1.tiff:0024-0589  0.000133   473.363   \n",
       "7     r01c02f01p01-ch1sk1fk1fl1.tiff:0033-0636  0.000149   457.824   \n",
       "8     r01c02f01p01-ch1sk1fk1fl1.tiff:0035-1027  0.002000   592.269   \n",
       "9     r01c02f01p01-ch1sk2fk1fl1.tiff:0004-0081  0.000064   542.825   \n",
       "10    r01c02f01p01-ch1sk2fk1fl1.tiff:0005-0264  0.000240   559.554   \n",
       "11    r01c02f01p01-ch1sk2fk1fl1.tiff:0011-0346  0.000155   572.660   \n",
       "12    r01c02f01p01-ch1sk2fk1fl1.tiff:0015-0406  0.000340   401.738   \n",
       "13    r01c02f01p01-ch1sk2fk1fl1.tiff:0017-0550  0.000369   571.778   \n",
       "14    r01c02f01p01-ch1sk2fk1fl1.tiff:0018-0568  0.000175   405.767   \n",
       "15    r01c02f01p01-ch1sk2fk1fl1.tiff:0024-0589  0.000133   512.597   \n",
       "16    r01c02f01p01-ch1sk2fk1fl1.tiff:0030-0632  0.000879   587.348   \n",
       "17    r01c02f01p01-ch1sk2fk1fl1.tiff:0033-0636  0.000149   455.757   \n",
       "18    r01c02f01p01-ch1sk2fk1fl1.tiff:0035-1027  0.002000   527.892   \n",
       "19   r01c02f01p01-ch1sk30fk1fl1.tiff:0004-0081  0.000064   475.091   \n",
       "20   r01c02f01p01-ch1sk30fk1fl1.tiff:0010-0343  0.000153   563.832   \n",
       "21   r01c02f01p01-ch1sk30fk1fl1.tiff:0015-0406  0.000340   465.597   \n",
       "22   r01c02f01p01-ch1sk30fk1fl1.tiff:0017-0550  0.000369   460.093   \n",
       "23   r01c02f01p01-ch1sk30fk1fl1.tiff:0018-0568  0.000175   525.599   \n",
       "24   r01c02f01p01-ch1sk30fk1fl1.tiff:0024-0589  0.000133   454.525   \n",
       "25   r01c02f01p01-ch1sk30fk1fl1.tiff:0030-0632  0.000879   554.246   \n",
       "26   r01c02f01p01-ch1sk30fk1fl1.tiff:0033-0636  0.000149   518.760   \n",
       "27   r01c02f01p01-ch1sk30fk1fl1.tiff:0035-1027  0.002000   488.006   \n",
       "28   r01c02f01p01-ch1sk31fk1fl1.tiff:0010-0343  0.000153   552.562   \n",
       "29   r01c02f01p01-ch1sk31fk1fl1.tiff:0015-0406  0.000340   466.748   \n",
       "..                                         ...       ...       ...   \n",
       "638  r02c02f01p01-ch1sk17fk1fl1.tiff:0012-0476  0.000552   365.396   \n",
       "639  r02c02f01p01-ch1sk17fk1fl1.tiff:0014-0511  0.000439   445.350   \n",
       "640  r02c02f01p01-ch1sk17fk1fl1.tiff:0019-0669  0.000382   493.125   \n",
       "641  r02c02f01p01-ch1sk17fk1fl1.tiff:0020-0684  0.000740   491.595   \n",
       "642  r02c02f01p01-ch1sk17fk1fl1.tiff:0021-0685  0.000900   518.010   \n",
       "643  r02c02f01p01-ch1sk17fk1fl1.tiff:0022-0686  0.000635   462.233   \n",
       "644  r02c02f01p01-ch1sk17fk1fl1.tiff:0030-0882  0.000298   375.736   \n",
       "645  r02c02f01p01-ch1sk17fk1fl1.tiff:0031-0879  0.000338   436.345   \n",
       "646  r02c02f01p01-ch1sk17fk1fl1.tiff:0033-0891  0.000340   366.053   \n",
       "647  r02c02f01p01-ch1sk17fk1fl1.tiff:0003-0069  0.000393   354.342   \n",
       "648  r02c02f01p01-ch1sk18fk1fl1.tiff:0008-0384  0.000774   434.758   \n",
       "649  r02c02f01p01-ch1sk18fk1fl1.tiff:0014-0511  0.000439   441.911   \n",
       "650  r02c02f01p01-ch1sk18fk1fl1.tiff:0019-0669  0.000382   497.399   \n",
       "651  r02c02f01p01-ch1sk18fk1fl1.tiff:0020-0684  0.000740   477.103   \n",
       "652  r02c02f01p01-ch1sk18fk1fl1.tiff:0021-0685  0.000900   486.860   \n",
       "653  r02c02f01p01-ch1sk18fk1fl1.tiff:0022-0686  0.000635   451.412   \n",
       "654  r02c02f01p01-ch1sk18fk1fl1.tiff:0030-0882  0.000298   376.321   \n",
       "655  r02c02f01p01-ch1sk18fk1fl1.tiff:0031-0879  0.000338   431.844   \n",
       "656  r02c02f01p01-ch1sk18fk1fl1.tiff:0033-0891  0.000340   352.996   \n",
       "657  r02c02f01p01-ch1sk18fk1fl1.tiff:0003-0069  0.000393   363.964   \n",
       "658  r02c02f01p01-ch1sk19fk1fl1.tiff:0008-0384  0.000774   442.850   \n",
       "659  r02c02f01p01-ch1sk19fk1fl1.tiff:0012-0476  0.000552   371.795   \n",
       "660  r02c02f01p01-ch1sk19fk1fl1.tiff:0014-0511  0.000439   461.214   \n",
       "661  r02c02f01p01-ch1sk19fk1fl1.tiff:0019-0669  0.000382   498.410   \n",
       "662  r02c02f01p01-ch1sk19fk1fl1.tiff:0020-0684  0.000740   499.659   \n",
       "663  r02c02f01p01-ch1sk19fk1fl1.tiff:0021-0685  0.000900   518.022   \n",
       "664  r02c02f01p01-ch1sk19fk1fl1.tiff:0022-0686  0.000635   465.980   \n",
       "665  r02c02f01p01-ch1sk19fk1fl1.tiff:0030-0882  0.000298   370.755   \n",
       "666  r02c02f01p01-ch1sk19fk1fl1.tiff:0031-0879  0.000338   438.303   \n",
       "667  r02c02f01p01-ch1sk19fk1fl1.tiff:0003-0069  0.000393   371.397   \n",
       "\n",
       "     INTDEN_GFP  MEDIAN_GFP  RAWINTDEN_GFP  row col  frame color_GFP  \\\n",
       "0         0.035         553          85290  r01  02  01p01       GFP   \n",
       "1         0.085         545         203461  r01  02  01p01       GFP   \n",
       "2         0.079         453         189427  r01  02  01p01       GFP   \n",
       "3         0.160         471         383620  r01  02  01p01       GFP   \n",
       "4         0.202         553         485588  r01  02  01p01       GFP   \n",
       "5         0.085         488         204207  r01  02  01p01       GFP   \n",
       "6         0.063         473         151476  r01  02  01p01       GFP   \n",
       "7         0.068         450         163901  r01  02  01p01       GFP   \n",
       "8         1.228         581        2952460  r01  02  01p01       GFP   \n",
       "9         0.035         545          83595  r01  02  01p01       GFP   \n",
       "10        0.134         581         322303  r01  02  01p01       GFP   \n",
       "11        0.089         570         213602  r01  02  01p01       GFP   \n",
       "12        0.137         429         328622  r01  02  01p01       GFP   \n",
       "13        0.211         580         506595  r01  02  01p01       GFP   \n",
       "14        0.071         422         170828  r01  02  01p01       GFP   \n",
       "15        0.068         520         164031  r01  02  01p01       GFP   \n",
       "16        0.516         543        1240478  r01  02  01p01       GFP   \n",
       "17        0.068         464         163161  r01  02  01p01       GFP   \n",
       "18        1.095         524        2631542  r01  02  01p01       GFP   \n",
       "19        0.030         481          73164  r01  02  01p01       GFP   \n",
       "20        0.086         568         207490  r01  02  01p01       GFP   \n",
       "21        0.158         465         380858  r01  02  01p01       GFP   \n",
       "22        0.170         447         407642  r01  02  01p01       GFP   \n",
       "23        0.092         564         221277  r01  02  01p01       GFP   \n",
       "24        0.061         467         145448  r01  02  01p01       GFP   \n",
       "25        0.487         508        1170568  r01  02  01p01       GFP   \n",
       "26        0.077         528         185716  r01  02  01p01       GFP   \n",
       "27        1.012         487        2432708  r01  02  01p01       GFP   \n",
       "28        0.085         551         203343  r01  02  01p01       GFP   \n",
       "29        0.159         466         381800  r01  02  01p01       GFP   \n",
       "..          ...         ...            ...  ...  ..    ...       ...   \n",
       "638       0.202         366         484881  r02  02  01p01       GFP   \n",
       "639       0.196         471         470290  r02  02  01p01       GFP   \n",
       "640       0.188         509         452196  r02  02  01p01       GFP   \n",
       "641       0.364         503         874548  r02  02  01p01       GFP   \n",
       "642       0.466         510        1120974  r02  02  01p01       GFP   \n",
       "643       0.293         478         704905  r02  02  01p01       GFP   \n",
       "644       0.112         379         269403  r02  02  01p01       GFP   \n",
       "645       0.147         432         354312  r02  02  01p01       GFP   \n",
       "646       0.125         364         299431  r02  02  01p01       GFP   \n",
       "647       0.139         373         334499  r02  02  01p01       GFP   \n",
       "648       0.337         446         809085  r02  02  01p01       GFP   \n",
       "649       0.194         470         466658  r02  02  01p01       GFP   \n",
       "650       0.190         509         456115  r02  02  01p01       GFP   \n",
       "651       0.353         479         848766  r02  02  01p01       GFP   \n",
       "652       0.438         482        1053566  r02  02  01p01       GFP   \n",
       "653       0.286         463         688404  r02  02  01p01       GFP   \n",
       "654       0.112         385         269822  r02  02  01p01       GFP   \n",
       "655       0.146         425         350657  r02  02  01p01       GFP   \n",
       "656       0.120         367         288751  r02  02  01p01       GFP   \n",
       "657       0.143         382         343582  r02  02  01p01       GFP   \n",
       "658       0.343         457         824143  r02  02  01p01       GFP   \n",
       "659       0.205         370         493372  r02  02  01p01       GFP   \n",
       "660       0.203         488         487042  r02  02  01p01       GFP   \n",
       "661       0.190         509         457042  r02  02  01p01       GFP   \n",
       "662       0.370         517         888893  r02  02  01p01       GFP   \n",
       "663       0.466         516        1121000  r02  02  01p01       GFP   \n",
       "664       0.296         475         710620  r02  02  01p01       GFP   \n",
       "665       0.111         374         265831  r02  02  01p01       GFP   \n",
       "666       0.148         435         355902  r02  02  01p01       GFP   \n",
       "667       0.146         386         350599  r02  02  01p01       GFP   \n",
       "\n",
       "       ...          imagename condition_name  construct_name  \\\n",
       "0      ...     tiff:0004-0081           DMSO  SBE4 construct   \n",
       "1      ...     tiff:0011-0346           DMSO  SBE4 construct   \n",
       "2      ...     tiff:0012-0360           DMSO  SBE4 construct   \n",
       "3      ...     tiff:0015-0406           DMSO  SBE4 construct   \n",
       "4      ...     tiff:0017-0550           DMSO  SBE4 construct   \n",
       "5      ...     tiff:0018-0568           DMSO  SBE4 construct   \n",
       "6      ...     tiff:0024-0589           DMSO  SBE4 construct   \n",
       "7      ...     tiff:0033-0636           DMSO  SBE4 construct   \n",
       "8      ...     tiff:0035-1027           DMSO  SBE4 construct   \n",
       "9      ...     tiff:0004-0081           DMSO  SBE4 construct   \n",
       "10     ...     tiff:0005-0264           DMSO  SBE4 construct   \n",
       "11     ...     tiff:0011-0346           DMSO  SBE4 construct   \n",
       "12     ...     tiff:0015-0406           DMSO  SBE4 construct   \n",
       "13     ...     tiff:0017-0550           DMSO  SBE4 construct   \n",
       "14     ...     tiff:0018-0568           DMSO  SBE4 construct   \n",
       "15     ...     tiff:0024-0589           DMSO  SBE4 construct   \n",
       "16     ...     tiff:0030-0632           DMSO  SBE4 construct   \n",
       "17     ...     tiff:0033-0636           DMSO  SBE4 construct   \n",
       "18     ...     tiff:0035-1027           DMSO  SBE4 construct   \n",
       "19     ...     tiff:0004-0081           DMSO  SBE4 construct   \n",
       "20     ...     tiff:0010-0343           DMSO  SBE4 construct   \n",
       "21     ...     tiff:0015-0406           DMSO  SBE4 construct   \n",
       "22     ...     tiff:0017-0550           DMSO  SBE4 construct   \n",
       "23     ...     tiff:0018-0568           DMSO  SBE4 construct   \n",
       "24     ...     tiff:0024-0589           DMSO  SBE4 construct   \n",
       "25     ...     tiff:0030-0632           DMSO  SBE4 construct   \n",
       "26     ...     tiff:0033-0636           DMSO  SBE4 construct   \n",
       "27     ...     tiff:0035-1027           DMSO  SBE4 construct   \n",
       "28     ...     tiff:0010-0343           DMSO  SBE4 construct   \n",
       "29     ...     tiff:0015-0406           DMSO  SBE4 construct   \n",
       "..     ...                ...            ...             ...   \n",
       "638    ...     tiff:0012-0476   Gherlin 75nM  SBE4 construct   \n",
       "639    ...     tiff:0014-0511   Gherlin 75nM  SBE4 construct   \n",
       "640    ...     tiff:0019-0669   Gherlin 75nM  SBE4 construct   \n",
       "641    ...     tiff:0020-0684   Gherlin 75nM  SBE4 construct   \n",
       "642    ...     tiff:0021-0685   Gherlin 75nM  SBE4 construct   \n",
       "643    ...     tiff:0022-0686   Gherlin 75nM  SBE4 construct   \n",
       "644    ...     tiff:0030-0882   Gherlin 75nM  SBE4 construct   \n",
       "645    ...     tiff:0031-0879   Gherlin 75nM  SBE4 construct   \n",
       "646    ...     tiff:0033-0891   Gherlin 75nM  SBE4 construct   \n",
       "647    ...     tiff:0003-0069   Gherlin 75nM  SBE4 construct   \n",
       "648    ...     tiff:0008-0384   Gherlin 75nM  SBE4 construct   \n",
       "649    ...     tiff:0014-0511   Gherlin 75nM  SBE4 construct   \n",
       "650    ...     tiff:0019-0669   Gherlin 75nM  SBE4 construct   \n",
       "651    ...     tiff:0020-0684   Gherlin 75nM  SBE4 construct   \n",
       "652    ...     tiff:0021-0685   Gherlin 75nM  SBE4 construct   \n",
       "653    ...     tiff:0022-0686   Gherlin 75nM  SBE4 construct   \n",
       "654    ...     tiff:0030-0882   Gherlin 75nM  SBE4 construct   \n",
       "655    ...     tiff:0031-0879   Gherlin 75nM  SBE4 construct   \n",
       "656    ...     tiff:0033-0891   Gherlin 75nM  SBE4 construct   \n",
       "657    ...     tiff:0003-0069   Gherlin 75nM  SBE4 construct   \n",
       "658    ...     tiff:0008-0384   Gherlin 75nM  SBE4 construct   \n",
       "659    ...     tiff:0012-0476   Gherlin 75nM  SBE4 construct   \n",
       "660    ...     tiff:0014-0511   Gherlin 75nM  SBE4 construct   \n",
       "661    ...     tiff:0019-0669   Gherlin 75nM  SBE4 construct   \n",
       "662    ...     tiff:0020-0684   Gherlin 75nM  SBE4 construct   \n",
       "663    ...     tiff:0021-0685   Gherlin 75nM  SBE4 construct   \n",
       "664    ...     tiff:0022-0686   Gherlin 75nM  SBE4 construct   \n",
       "665    ...     tiff:0030-0882   Gherlin 75nM  SBE4 construct   \n",
       "666    ...     tiff:0031-0879   Gherlin 75nM  SBE4 construct   \n",
       "667    ...     tiff:0003-0069   Gherlin 75nM  SBE4 construct   \n",
       "\n",
       "                                      LABEL_RFP MEAN_RFP  INTDEN_RFP  \\\n",
       "0    r01c02f01p01-ch2sk1fk1fl1-1.tiff:0004-0081  489.636       0.031   \n",
       "1    r01c02f01p01-ch2sk1fk1fl1-1.tiff:0011-0346  681.627       0.106   \n",
       "2    r01c02f01p01-ch2sk1fk1fl1-1.tiff:0012-0360  618.995       0.105   \n",
       "3    r01c02f01p01-ch2sk1fk1fl1-1.tiff:0015-0406  555.685       0.189   \n",
       "4    r01c02f01p01-ch2sk1fk1fl1-1.tiff:0017-0550  575.968       0.212   \n",
       "5    r01c02f01p01-ch2sk1fk1fl1-1.tiff:0018-0568  514.487       0.090   \n",
       "6    r01c02f01p01-ch2sk1fk1fl1-1.tiff:0024-0589  486.413       0.065   \n",
       "7    r01c02f01p01-ch2sk1fk1fl1-1.tiff:0033-0636  583.173       0.087   \n",
       "8    r01c02f01p01-ch2sk1fk1fl1-1.tiff:0035-1027  669.683       1.389   \n",
       "9      r01c02f01p01-ch2sk2fk1fl1.tiff:0004-0081  462.838       0.030   \n",
       "10     r01c02f01p01-ch2sk2fk1fl1.tiff:0005-0264  472.014       0.113   \n",
       "11     r01c02f01p01-ch2sk2fk1fl1.tiff:0011-0346  683.544       0.106   \n",
       "12     r01c02f01p01-ch2sk2fk1fl1.tiff:0015-0406  478.061       0.163   \n",
       "13     r01c02f01p01-ch2sk2fk1fl1.tiff:0017-0550  613.533       0.226   \n",
       "14     r01c02f01p01-ch2sk2fk1fl1.tiff:0018-0568  435.774       0.076   \n",
       "15     r01c02f01p01-ch2sk2fk1fl1.tiff:0024-0589  539.966       0.072   \n",
       "16     r01c02f01p01-ch2sk2fk1fl1.tiff:0030-0632  668.534       0.587   \n",
       "17     r01c02f01p01-ch2sk2fk1fl1.tiff:0033-0636  637.827       0.095   \n",
       "18     r01c02f01p01-ch2sk2fk1fl1.tiff:0035-1027  647.740       1.343   \n",
       "19    r01c02f01p01-ch2sk30fk1fl1.tiff:0004-0081  411.422       0.026   \n",
       "20    r01c02f01p01-ch2sk30fk1fl1.tiff:0010-0343  609.812       0.093   \n",
       "21    r01c02f01p01-ch2sk30fk1fl1.tiff:0015-0406  500.249       0.170   \n",
       "22    r01c02f01p01-ch2sk30fk1fl1.tiff:0017-0550  498.806       0.184   \n",
       "23    r01c02f01p01-ch2sk30fk1fl1.tiff:0018-0568  641.865       0.112   \n",
       "24    r01c02f01p01-ch2sk30fk1fl1.tiff:0024-0589  527.966       0.070   \n",
       "25    r01c02f01p01-ch2sk30fk1fl1.tiff:0030-0632  557.796       0.490   \n",
       "26    r01c02f01p01-ch2sk30fk1fl1.tiff:0033-0636  697.824       0.104   \n",
       "27    r01c02f01p01-ch2sk30fk1fl1.tiff:0035-1027  612.195       1.270   \n",
       "28    r01c02f01p01-ch2sk31fk1fl1.tiff:0010-0343  601.315       0.092   \n",
       "29    r01c02f01p01-ch2sk31fk1fl1.tiff:0015-0406  504.518       0.172   \n",
       "..                                          ...      ...         ...   \n",
       "638   r02c02f01p01-ch2sk17fk1fl1.tiff:0012-0476  412.456       0.228   \n",
       "639   r02c02f01p01-ch2sk17fk1fl1.tiff:0014-0511  484.253       0.213   \n",
       "640   r02c02f01p01-ch2sk17fk1fl1.tiff:0019-0669  533.277       0.203   \n",
       "641   r02c02f01p01-ch2sk17fk1fl1.tiff:0020-0684  524.469       0.388   \n",
       "642   r02c02f01p01-ch2sk17fk1fl1.tiff:0021-0685  559.112       0.503   \n",
       "643   r02c02f01p01-ch2sk17fk1fl1.tiff:0022-0686  617.284       0.392   \n",
       "644   r02c02f01p01-ch2sk17fk1fl1.tiff:0030-0882  455.165       0.136   \n",
       "645   r02c02f01p01-ch2sk17fk1fl1.tiff:0031-0879  586.560       0.198   \n",
       "646   r02c02f01p01-ch2sk17fk1fl1.tiff:0033-0891  474.610       0.162   \n",
       "647   r02c02f01p01-ch2sk17fk1fl1.tiff:0003-0069  477.731       0.188   \n",
       "648   r02c02f01p01-ch2sk18fk1fl1.tiff:0008-0384  563.090       0.436   \n",
       "649   r02c02f01p01-ch2sk18fk1fl1.tiff:0014-0511  465.562       0.205   \n",
       "650   r02c02f01p01-ch2sk18fk1fl1.tiff:0019-0669  525.501       0.200   \n",
       "651   r02c02f01p01-ch2sk18fk1fl1.tiff:0020-0684  489.070       0.362   \n",
       "652   r02c02f01p01-ch2sk18fk1fl1.tiff:0021-0685  499.098       0.449   \n",
       "653   r02c02f01p01-ch2sk18fk1fl1.tiff:0022-0686  573.012       0.364   \n",
       "654   r02c02f01p01-ch2sk18fk1fl1.tiff:0030-0882  456.767       0.136   \n",
       "655   r02c02f01p01-ch2sk18fk1fl1.tiff:0031-0879  564.272       0.191   \n",
       "656   r02c02f01p01-ch2sk18fk1fl1.tiff:0033-0891  434.361       0.148   \n",
       "657   r02c02f01p01-ch2sk18fk1fl1.tiff:0003-0069  473.531       0.186   \n",
       "658   r02c02f01p01-ch2sk19fk1fl1.tiff:0008-0384  611.382       0.473   \n",
       "659   r02c02f01p01-ch2sk19fk1fl1.tiff:0012-0476  437.625       0.242   \n",
       "660   r02c02f01p01-ch2sk19fk1fl1.tiff:0014-0511  500.688       0.220   \n",
       "661   r02c02f01p01-ch2sk19fk1fl1.tiff:0019-0669  553.228       0.211   \n",
       "662   r02c02f01p01-ch2sk19fk1fl1.tiff:0020-0684  558.211       0.413   \n",
       "663   r02c02f01p01-ch2sk19fk1fl1.tiff:0021-0685  583.122       0.525   \n",
       "664   r02c02f01p01-ch2sk19fk1fl1.tiff:0022-0686  641.907       0.407   \n",
       "665   r02c02f01p01-ch2sk19fk1fl1.tiff:0030-0882  464.840       0.139   \n",
       "666   r02c02f01p01-ch2sk19fk1fl1.tiff:0031-0879  611.695       0.207   \n",
       "667   r02c02f01p01-ch2sk19fk1fl1.tiff:0003-0069  515.318       0.202   \n",
       "\n",
       "     MEDIAN_RFP  RAWINTDEN_RFP  color_RFP     ratio  \n",
       "0           486          75404        RFP  1.137860  \n",
       "1           685         254247        RFP  0.795620  \n",
       "2           622         252550        RFP  0.728296  \n",
       "3           556         454550        RFP  0.847122  \n",
       "4           576         510308        RFP  0.960069  \n",
       "5           515         216599        RFP  0.947573  \n",
       "6           484         155652        RFP  0.977273  \n",
       "7           573         208776        RFP  0.785340  \n",
       "8           640        3338371        RFP  0.907813  \n",
       "9           466          71277        RFP  1.169528  \n",
       "10          477         271880        RFP  1.218029  \n",
       "11          676         254962        RFP  0.843195  \n",
       "12          512         391054        RFP  0.837891  \n",
       "13          627         543590        RFP  0.925040  \n",
       "14          438         183461        RFP  0.963470  \n",
       "15          543         172789        RFP  0.957643  \n",
       "16          688        1411944        RFP  0.789244  \n",
       "17          665         228342        RFP  0.697744  \n",
       "18          624        3228983        RFP  0.839744  \n",
       "19          412          63359        RFP  1.167476  \n",
       "20          636         224411        RFP  0.893082  \n",
       "21          514         409204        RFP  0.904669  \n",
       "22          493         441942        RFP  0.906694  \n",
       "23          694         270225        RFP  0.812680  \n",
       "24          534         168949        RFP  0.874532  \n",
       "25          555        1178066        RFP  0.915315  \n",
       "26          713         249821        RFP  0.740533  \n",
       "27          627        3051794        RFP  0.776715  \n",
       "28          593         221284        RFP  0.929174  \n",
       "29          512         412696        RFP  0.910156  \n",
       "..          ...            ...        ...       ...  \n",
       "638         422         547329        RFP  0.867299  \n",
       "639         516         511371        RFP  0.912791  \n",
       "640         554         489015        RFP  0.918773  \n",
       "641         516         933031        RFP  0.974806  \n",
       "642         536        1209918        RFP  0.951493  \n",
       "643         634         941358        RFP  0.753943  \n",
       "644         480         326353        RFP  0.789583  \n",
       "645         599         476287        RFP  0.721202  \n",
       "646         521         388231        RFP  0.698656  \n",
       "647         511         450978        RFP  0.729941  \n",
       "648         578        1047911        RFP  0.771626  \n",
       "649         492         491633        RFP  0.955285  \n",
       "650         547         481884        RFP  0.930530  \n",
       "651         461         870055        RFP  1.039046  \n",
       "652         477        1080049        RFP  1.010482  \n",
       "653         578         873843        RFP  0.801038  \n",
       "654         485         327502        RFP  0.793814  \n",
       "655         575         458189        RFP  0.739130  \n",
       "656         439         355307        RFP  0.835991  \n",
       "657         496         447013        RFP  0.770161  \n",
       "658         644        1137782        RFP  0.709627  \n",
       "659         451         580728        RFP  0.820399  \n",
       "660         542         528727        RFP  0.900369  \n",
       "661         573         507310        RFP  0.888307  \n",
       "662         565         993057        RFP  0.915044  \n",
       "663         565        1261877        RFP  0.913274  \n",
       "664         667         978908        RFP  0.712144  \n",
       "665         481         333290        RFP  0.777547  \n",
       "666         617         496696        RFP  0.705024  \n",
       "667         546         486460        RFP  0.706960  \n",
       "\n",
       "[668 rows x 21 columns]"
      ]
     },
     "execution_count": 170,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "mdf"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 171,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T18:08:29.453157Z",
     "start_time": "2021-05-18T18:08:29.443161Z"
    }
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "Gherlin 75nM    389\n",
       "DMSO            279\n",
       "Name: condition_name, dtype: int64"
      ]
     },
     "execution_count": 171,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "mdf[\"condition_name\"].value_counts()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ghrelin"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 172,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T18:08:30.926264Z",
     "start_time": "2021-05-18T18:08:30.858397Z"
    }
   },
   "outputs": [],
   "source": [
    "#https://plotly.com/python/violin/\n",
    "\n",
    "\n",
    "# def graphaparamater(graphof, treatment):\n",
    "def graphaparamater(graphof):\n",
    "    whichtreatment = treatment_names[1]\n",
    "#     print(whichtreatment)\n",
    "    controltreatment = treatment_names[0]\n",
    "#     print(controltreatment)\n",
    "    controlrowdf = mdf[mdf[\"condition_name\"]==controltreatment]\n",
    "    onerowdf = mdf[mdf[\"condition_name\"]==whichtreatment]\n",
    "\n",
    "    onetypedf = pandas.concat([controlrowdf, onerowdf])\n",
    "#     onetypedf = onetypedf[onetypedf['condition_name']==treatment]\n",
    "#     print(onerowdf)\n",
    "\n",
    "    fig = go.Figure()\n",
    "    fig.add_trace(go.Violin(x=onetypedf['time'][ onetypedf['condition_name'] == controltreatment],\n",
    "                            y=onetypedf[graphof][ onetypedf['condition_name'] == controltreatment],\n",
    "                            legendgroup=controltreatment, scalegroup=controltreatment, name=controltreatment,\n",
    "                            side='negative',\n",
    "                            line_color='green', points='all')\n",
    "                 )\n",
    "    fig.add_trace(go.Violin(x=onetypedf['time'][ onetypedf['condition_name'] == whichtreatment ],\n",
    "                            y=onetypedf[graphof][ onetypedf['condition_name'] == whichtreatment ],\n",
    "                            legendgroup=whichtreatment, scalegroup=whichtreatment, name=whichtreatment,\n",
    "                            side='positive',\n",
    "                            line_color='orange', points='all')\n",
    "                 )    \n",
    "    \n",
    "    \n",
    "               \n",
    "    fig.update_traces(meanline_visible=True)\n",
    "# #     title=graphof+' '+treatment\n",
    "#     fig.update_layout(violingap=0, violinmode='overlay',title=title)\n",
    "    fig.update_layout(violingap=0, violinmode='overlay')\n",
    "    fig.show()\n"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 173,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T18:08:31.895117Z",
     "start_time": "2021-05-18T18:08:31.824070Z"
    }
   },
   "outputs": [
    {
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   ],
   "source": [
    "graphof = \"MEDIAN_RFP\"\n",
    "graphaparamater(graphof)\n",
    "\n",
    "# treatment = 'DMSO'\n",
    "# treatment = 'Gherlin 75nM'\n",
    "# graphaparamater(graphof,treatment)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 174,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T18:08:33.750702Z",
     "start_time": "2021-05-18T18:08:33.683554Z"
    }
   },
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   ],
   "source": [
    "graphof = \"MEDIAN_GFP\"\n",
    "graphaparamater(graphof)\n",
    "# treatment = 'DMSO'\n",
    "# graphaparamater(graphof,treatment)\n",
    "# treatment = 'gherlin 75nM'\n",
    "# graphaparamater(graphof,treatment)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 175,
   "metadata": {
    "ExecuteTime": {
     "end_time": "2021-05-18T18:08:34.804043Z",
     "start_time": "2021-05-18T18:08:34.717768Z"
    }
   },
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   "source": [
    "graphof = \"ratio\"\n",
    "graphaparamater(graphof)\n",
    "# treatment = 'DMSO'\n",
    "# graphaparamater(graphof,treatment)\n",
    "# treatment = 'gherlin 75nM'\n",
    "# graphaparamater(graphof,treatment)"
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