10x V3 Chemistry

[aelias1]

Unfortunately, DropEst is unable to work correctly with 10x V3. Should I simply use the new unfiltered barcodes for V3 and what other changes must be made?

[aelias1]

I recently posted on github: I use the code below immediately following my loading in data - which is shown in your documentation:_cds <- preprocess_cds(_cds, num_dim = 100)
_cds <- reduce_dimension(_cds)
plot_cells(_cds)I receive the error:
No trajectory to plot. Has learn_graph() been called yet?
cluster not found in colData(cds), cells will not be colored
cluster_cells() has not been called yet, can't color cells by clusterI cannot find in your Monocle 3 documentation these commands...at least to use before the commands stated above. I am using in data loaded from 10x
the Response I received was that I should use learn graph first.  However, your documentation did no thave this and led me to use the code I used. Do you have updated documentation? Thank you. 

[chlee-tabin]

See this: #105 (comment)