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wondering about merging steps #120

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kkamiii opened this issue Oct 18, 2020 · 0 comments

kkamiii commented Oct 18, 2020 •

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hello :)

Thanks for allowing me to use your great tool 👍

I'm wondering about merging steps.

After read your thesis and documentation, I understand merging precedure like below..

UMI correction
->merging different sequence UMI which originally same sequence UMI but differentiated by sequencing error or any other reasons.
CB Correction
-> merging Cellular barcode that have similar CB (contain some of the same gene–UMI combinations)

but, when I check est_main.log information .... merging precedure was different from what I knew.

Why does the UMI merging step start after the CB merging ?

Am I misunderstanding?

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