diff --git a/python/lsst/daf/butler/datastores/inMemoryDatastore.py b/python/lsst/daf/butler/datastores/inMemoryDatastore.py
index a09b7d080d..eff6e86d88 100644
--- a/python/lsst/daf/butler/datastores/inMemoryDatastore.py
+++ b/python/lsst/daf/butler/datastores/inMemoryDatastore.py
@@ -39,6 +39,7 @@
 from urllib.parse import urlencode
 
 from lsst.daf.butler import DatasetId, DatasetRef, StorageClass
+from lsst.daf.butler._exceptions import DatasetTypeNotSupportedError
 from lsst.daf.butler.datastore import DatasetRefURIs, DatastoreConfig
 from lsst.daf.butler.datastore.generic_base import GenericBaseDatastore, post_process_get
 from lsst.daf.butler.datastore.record_data import DatastoreRecordData
@@ -407,7 +408,11 @@ def put(self, inMemoryDataset: Any, ref: DatasetRef) -> None:
         if not delegate or not delegate.can_accept(inMemoryDataset):
             inMemoryDataset = ref.datasetType.storageClass.coerce_type(inMemoryDataset)
 
-        self._validate_put_parameters(inMemoryDataset, ref)
+        if not self.constraints.isAcceptable(ref):
+            # Raise rather than use boolean return value.
+            raise DatasetTypeNotSupportedError(
+                f"Dataset {ref} has been rejected by this datastore via configuration."
+            )
 
         self.datasets[ref.id] = inMemoryDataset
         log.debug("Store %s in %s", ref, self.name)