diff --git a/scrnabox.slurm/hto/pars/step5_par.txt b/scrnabox.slurm/hto/pars/step5_par.txt index d2c7062..a02f4cc 100644 --- a/scrnabox.slurm/hto/pars/step5_par.txt +++ b/scrnabox.slurm/hto/pars/step5_par.txt @@ -22,49 +22,52 @@ par_one_seurat= "no" ############################################################################ # If you have multiple Seurat objects, choose whether you want to integrate or merge the objects ############################################################################ +## integrate Seurat objects par_integrate_seurat= "yes" -par_merge_seurat= "no" - -############################################################################ -# Parameters for integration -############################################################################ -## Which dimensions to use from the CCA to specify the neighbour search space -par_FindIntegrationAnchors_dim= 25 +## merge Seurat objects +par_merge_seurat= "no" ############################################################################ # Parameters for normalization and scaling -# even if you opt to skip integration, adjust the following parameters +# Even if you opt to skip integration, adjust the following parameters ############################################################################ -## Assay to perform normalization and scaling. For most use cases this will be RNA +## Assay to perform normalization and scaling (prior to integration). For most use cases this will be RNA par_DefaultAssay= "RNA" ## Normalization method par_normalization.method= "LogNormalize" -## Scale factor for normalization +## Scale factor par_scale.factor= 10000 + +############################################################################ +# Parameters for integration +############################################################################ ## Method for detecting top variable features. vst, mean.var.plot (mvp), dispersion (disp) par_selection.method= "vst" -## Number of features to select as top variable features +## Number of features to select as top variable features for integration par_nfeatures= 2500 +## Which dimensions to use from the CCA to specify the neighbour search space +par_FindIntegrationAnchors_dim= 25 + ############################################################################ # Parameters for linear dimensional reduction # even if you opt to skip integration, adjust the following parameters ############################################################################ -## The number of neighbouring points used in local approximations of manifold structure -par_RunUMAP_n.neighbors= 65 - ## Total Number of PCs to compute and store for RunPCA par_RunPCA_npcs= 30 -## Which dimensions to use as input features for RunUMAP -par_RunUMAP_dims= 30 +## Which dimensions to use as input features for RunUMAP +par_RunUMAP_dims= 25 + +## The number of neighbouring points used in local approximations of manifold structure. +par_RunUMAP_n.neighbors= 45 -## whether or not to perform JackStraw computation. This computation takes a long time. +## Whether or not to perform JackStraw computation. This computation takes a long time. par_compute_jackstraw= "no"