single end, input fail fusion calling

[charbel2998]

Description of the bug

I have single end RNA seq, I created the sample sheet and kept the fastq_2 empty:
sample,fastq_1,fastq_2,strandedness
432,432.fastq.gz,,unstranded
6151,6151.fastq.gz,,unstranded

No matter how I change the sample sheet, it keeps asking for fast1_2,

any input?

Command used and terminal output

nextflow run nf-core/rnafusion -r 3.0.2 -profile singularity --input ~/block_storage/fusionsamples.csv --genomes_base ~/block_storage/humangenome/ --outdir ~/block_storage/humangenome/fusioncalling --max_memory '80 GB' --max_cpus 30



ERROR ~ ERROR: Validation of 'input' file failed!

 -- Check '.nextflow.log' file for details
The following errors have been detected:

* -- Entry 1: Missing required value: fastq_2
* -- Entry 2: Missing required value: fastq_2

 -- Check script '/home/ubuntu/.nextflow/assets/nf-core/rnafusion/./workflows/../subworkflows/local/utils_nfcore_rnafusion_pipeline/../../nf-core/utils_nfvalidation_plugin/main.nf' at line: 57 or see '.nextflow.log' file for more details

Relevant files

No response

System information

No response

[tdanhorn]

See also https://nfcore.slack.com/archives/CGB1CL0SC/p1714404757300169, which includes the solution.