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When launching this workflow :
nextflow run nf-core/spatialvi -r dev \ -profile docker \ --input /shared/samplesheet_spaceranger.csv \ --spaceranger_probeset /shared/Visium_Human_Transcriptome_Probe_Set_v1.0_GRCh38-2020-A.csv \ --spaceranger_reference /shared/homo_sapiens_chr22_reference.tar.gz \ --qc_min_counts 5 \ --qc_min_genes 3 \ --outdir /shared/test_output
I had this error at the MultiQC step :
/// MultiQC 🔍 | v1.21 | multiqc | MultiQC Version v1.23 now available! | multiqc | Search path : /home/alanl/work/c8/6af00af8c6888f021e0e867cbe27d2 | searching | ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 100% 61/61 | custom_content | nf-core-spatialvi-summary: Found 1 sample (html) | custom_content | nf-core-spatialvi-methods-description: Found 1 sample (html) | fastqc | Found 2 reports ╭────────────── Oops! The 'spaceranger' MultiQC module broke... ───────────────╮ │ Please copy this log and report it at │ │ https://github.com/MultiQC/MultiQC/issues │ │ Please attach a file that triggers the error. The last file found was: │ │ 52/web_summary.html │ │ │ │ Traceback (most recent call last): │ │ File "/usr/local/lib/python3.11/site-packages/multiqc/multiqc.py", line 75 │ │ output = mod() │ │ ^^^^^ │ │ File "/usr/local/lib/python3.11/site-packages/multiqc/modules/spaceranger/ │ │ n["count"] = self.parse_count_html() │ │ ^^^^^^^^^^^^^^^^^^^^^^^ │ │ File "/usr/local/lib/python3.11/site-packages/multiqc/modules/spaceranger/ │ │ description=self.spacerangercount_plots_conf["genomic_dna"]["description │ │ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~^^^^^^^^^^^^^^^ │ │ KeyError: 'genomic_dna' │ │ │ ╰──────────────────────────────────────────────────────────────────────────────╯ | multiqc | Report : multiqc_report.html | multiqc | Data : multiqc_data | multiqc | Plots : multiqc_plots | multiqc | MultiQC complete
Looking up issues on Github I found this was related to this issue (MultiQC/MultiQC#2428) that was fixed in 1.22 (https://github.com/MultiQC/MultiQC/releases/tag/v1.22)
I then forked spatialvi and updated the multiqc version on my fork and it worked : Alan-pad@10776c3
nextflow run nf-core/spatialvi -r dev \ -profile docker \ --input /shared/samplesheet_spaceranger.csv \ --spaceranger_probeset /shared/Visium_Human_Transcriptome_Probe_Set_v1.0_GRCh38-2020-A.csv \ --spaceranger_reference /shared/homo_sapiens_chr22_reference.tar.gz \ --qc_min_counts 5 \ --qc_min_genes 3 \ --outdir /shared/test_output /// MultiQC 🔍 | v1.21 | multiqc | MultiQC Version v1.23 now available! | multiqc | Search path : /home/alanl/work/c8/6af00af8c6888f021e0e867cbe27d2 | searching | ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 100% 61/61 | custom_content | nf-core-spatialvi-summary: Found 1 sample (html) | custom_content | nf-core-spatialvi-methods-description: Found 1 sample (html) | fastqc | Found 2 reports ╭────────────── Oops! The 'spaceranger' MultiQC module broke... ───────────────╮ │ Please copy this log and report it at │ │ https://github.com/MultiQC/MultiQC/issues │ │ Please attach a file that triggers the error. The last file found was: │ │ 52/web_summary.html │ │ │ │ Traceback (most recent call last): │ │ File "/usr/local/lib/python3.11/site-packages/multiqc/multiqc.py", line 75 │ │ output = mod() │ │ ^^^^^ │ │ File "/usr/local/lib/python3.11/site-packages/multiqc/modules/spaceranger/ │ │ n["count"] = self.parse_count_html() │ │ ^^^^^^^^^^^^^^^^^^^^^^^ │ │ File "/usr/local/lib/python3.11/site-packages/multiqc/modules/spaceranger/ │ │ description=self.spacerangercount_plots_conf["genomic_dna"]["description │ │ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~^^^^^^^^^^^^^^^ │ │ KeyError: 'genomic_dna' │ │ │ ╰──────────────────────────────────────────────────────────────────────────────╯ | multiqc | Report : multiqc_report.html | multiqc | Data : multiqc_data | multiqc | Plots : multiqc_plots | multiqc | MultiQC complete
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The text was updated successfully, but these errors were encountered:
Thanks for the reminder! The MultiQC module wasn't updated the last time I was working on this, but now it is. This should now work on the dev branch.
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Description of the bug
When launching this workflow :
I had this error at the MultiQC step :
Looking up issues on Github I found this was related to this issue (MultiQC/MultiQC#2428) that was fixed in 1.22 (https://github.com/MultiQC/MultiQC/releases/tag/v1.22)
I then forked spatialvi and updated the multiqc version on my fork and it worked : Alan-pad@10776c3
Command used and terminal output
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered: