diff --git a/lib/internal/test_runner/harness.js b/lib/internal/test_runner/harness.js
index b13ca112e5cc27..504cc1e93acd7a 100644
--- a/lib/internal/test_runner/harness.js
+++ b/lib/internal/test_runner/harness.js
@@ -25,6 +25,7 @@ const {
   setupTestReporters,
   shouldColorizeTestFiles,
 } = require('internal/test_runner/utils');
+const { queueMicrotask } = require('internal/process/task_queues');
 const { bigint: hrtime } = process.hrtime;
 
 const testResources = new SafeMap();
@@ -181,6 +182,7 @@ function setup(root) {
 
   root.harness = {
     __proto__: null,
+    allowTestsToRun: false,
     bootstrapComplete: false,
     coverage: FunctionPrototypeBind(collectCoverage, null, root, coverage),
     counters: {
@@ -219,7 +221,16 @@ function getGlobalRoot() {
 
 async function startSubtest(subtest) {
   await reportersSetup;
-  getGlobalRoot().harness.bootstrapComplete = true;
+
+  const root = getGlobalRoot();
+  if (!root.harness.bootstrapComplete) {
+    root.harness.bootstrapComplete = true;
+    queueMicrotask(() => {
+      root.harness.allowTestsToRun = true;
+      root.processPendingSubtests();
+    });
+  }
+
   await subtest.start();
 }
 
diff --git a/lib/internal/test_runner/runner.js b/lib/internal/test_runner/runner.js
index b7ac5756dad195..41fbfdab931392 100644
--- a/lib/internal/test_runner/runner.js
+++ b/lib/internal/test_runner/runner.js
@@ -538,6 +538,7 @@ function run(options = kEmptyObject) {
   }
   const runFiles = () => {
     root.harness.bootstrapComplete = true;
+    root.harness.allowTestsToRun = true;
     return SafePromiseAllSettledReturnVoid(testFiles, (path) => {
       const subtest = runTestFile(path, filesWatcher, opts);
       filesWatcher?.runningSubtests.set(path, subtest);
diff --git a/lib/internal/test_runner/test.js b/lib/internal/test_runner/test.js
index 126949d2584e38..daa3fa29c8d037 100644
--- a/lib/internal/test_runner/test.js
+++ b/lib/internal/test_runner/test.js
@@ -599,7 +599,7 @@ class Test extends AsyncResource {
     // it. Otherwise, return a Promise to the caller and mark the test as
     // pending for later execution.
     this.reporter.enqueue(this.nesting, this.loc, this.name);
-    if (!this.parent.hasConcurrency()) {
+    if (!this.root.harness.allowTestsToRun || !this.parent.hasConcurrency()) {
       const deferred = createDeferredPromise();
 
       deferred.test = this;
diff --git a/test/fixtures/test-runner/output/source_mapped_locations.snapshot b/test/fixtures/test-runner/output/source_mapped_locations.snapshot
index 24c3ee8d113446..29b70fd0d08378 100644
--- a/test/fixtures/test-runner/output/source_mapped_locations.snapshot
+++ b/test/fixtures/test-runner/output/source_mapped_locations.snapshot
@@ -21,6 +21,9 @@ not ok 1 - fails
     *
     *
     *
+    *
+    *
+    *
   ...
 1..1
 # tests 1