diff --git a/Bio/Seq.py b/Bio/Seq.py
index 7c5fe58b223..c841e69e327 100644
--- a/Bio/Seq.py
+++ b/Bio/Seq.py
@@ -104,7 +104,7 @@ def __repr__(self):
             # Shows the last three letters as it is often useful to see if
             # there is a stop codon at the end of a sequence.
             # Note total length is 54+3+3=60
-            return f"{self.__class__.__name__}('{str(self)[:54]}...{str(self)[-3:]}')"
+            return f"{self.__class__.__name__}('{str(self[:54])}...{str(self[-3:])}')"
         else:
             return f"{self.__class__.__name__}({self._data!r})"