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<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"/>
<title>Gibson FASTA auto cloning</title>
</head>
<body>
<p><h1>Gibson FASTA auto cloning</h1></p>
<form enctype="multipart/form-data" action="/upload" method="post">
Upload FASTA (nucleotide CDS) File: <input type="file" name="filearg" />
<br />
or
<br />
Copy paste raw CDS nucleotide sequence (only 1), or multiple CDS nucleotide sequences in FASTA format:
<br />
<TEXTAREA name="fasta_text"
rows="20" cols="80"
tabindex="40">
</TEXTAREA>
<br />
Sequence ID:<input type="text" name="record_id" value="no_id">
<br>
Sequence name:<input type="text" name="record_name" value="no_name">
<br>
Sequence description:<input type="text" name="record_description" value="no_description">
<br />
(if providing a FASTA files of CDS sequences, the FASTA record descriptions will be used instead)
<br />
<br />
Primer prefix:<input type="text" name="primer_prefix" value="TRF_x">
<br>
Primer start index:<input type="text" name="primer_index" value="1234">
<br>
Starting well (if 96-well plate):<input type="text" name="starting_well" value="A1">
<br>
<br>
Destination plasmid:
<br/>
<input type="radio" name="plasmid_name" value="pHis8_4" checked> pHis8_4<br>
<input type="radio" name="plasmid_name" value="pHis8_4B" checked> pHis8_4B<br>
<input type="radio" name="plasmid_name" value="p4XX"> p4XX yeast vectors<br>
<input type="radio" name="plasmid_name" value="PVP57K">PVP57K<br>
<input type="radio" name="plasmid_name" value="pTYB12">pTYB12<br>
<input type="radio" name="plasmid_name" value="pYTK001">pYTK001 Type3S part (no tags or fusions) - Golden Gate Assembly<br>
<input type="radio" name="plasmid_name" value="pEAQ-HT">pEAQ-HT (XhoI, AgeI)<br>
<input type="radio" name="plasmid_name" value="L4440">L4440<br>
<br>
IDT output format:
<input type="radio" name="IDT_format" value="single" checked>single primers
<input type="radio" name="IDT_format" value="plate">96 well plate<br>
<input type="submit" value="Submit" />
</form>
<a title="Server source code" href="https://github.com/photocyte/auto_clone">Server source code</a>
</body>
</html>