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2)Add a text file to the ./plasmids directory, with the name of the file before the suffix equal to the value from the previous step
e.g.
pNew1.txt, with the following inside:
ForwardOverlap:GGGGGTTTTTAAACCCCC
ReverseOverlap:TTTTTTCCCCCAAAAGGGG
Comment:pNew1 Gibson primer for :
Don't screw up colons etc.
3)Thats the minimum needed. To have the script make the .gb plasmid files automatically, add a .gb file with several Ns to indicate where the insert should go
e.g.
pNew1.gb, and inside it has capital Ns designating the insert: