From d24daae4a8d51b8ff3e2e10506ffe78bcd57582a Mon Sep 17 00:00:00 2001 From: Tony Zhou <386346852@qq.com> Date: Fri, 21 Jun 2024 14:21:28 +0800 Subject: [PATCH] Update usage.md --- docs/usage.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index b8de516..21411e9 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -93,7 +93,7 @@ nf-core launch singleron-RD/sccite ### Tag barcode -`tag_barcode_fasta` Path to the tag barcode fasta file. The read name in the fasta is the antibody ID and the sequence is the tag barcode. Example: +`tag_barcode_fasta` Path to the tag barcode fasta file. The read names in the fasta are the antibody ID and the sequences are the tag barcodes. Example: ``` >A0001_CD4 AACAAGACCCTTGAG @@ -101,7 +101,7 @@ AACAAGACCCTTGAG TACCCGTAATAGCGT ``` -`r2_pattern` Specifies how to extract the index barcode sequence from the R2 read. Example: `L21C15` means the first 21 bases are the linker, the tag barcode starts at the 22nd base and is 15 bases long. +`r2_pattern` Specifies how to locate the tag barcode sequence in R2 reads. Example: `L21C15` means the first 21 bases are the linker, the tag barcode starts at the 22nd base and is 15 bases long. ### Running the pipeline with test data