Some erroneous output alongwith correct results

[GurpreethgnisBayer]

Hi,
I have been trying to run CAS-OFFINDER using the binary files that i have downloaded from https://github.com/snugel/cas-offinder/releases/tag/3.0.0b3.

The input to the binary file is as follows

var/chromosome/human_hg38
NNNNNNNNNNNNNNNNNNNNNGG 0 0
GACCCCCTCCACCCCGCCTCNNN 5 Seq0

Looking at the output, i see it does expected output for the test case, GACCCCCTCCACCCCGCCTC with selected PAM as SpCas9 from Streptococcus pyogenes: 5'-NGG-3'. However, there are some erroneous rows as shown below

#Id	Bulge Type	crRNA	DNA	Chromosome	Location	Direction	Mismatches	Bulge Size

Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GACCCCCTCCACCCCGCCTCCGG | chr6 | 43770818 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | cttaCataagtCgCaGatggTGG | chr16 | 90227832 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tgaCttacatAagtCGCagaTGG | chr16 | 90227835 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | aAaaCCacagtgtCtGtaTCTGG | chr16 | 90227868 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tttCtCactgttgaaGtgggAGG | chr16 | 90227801 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | agCtttCTCactgttGaagtGGG | chr16 | 90227804 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GAatagaatCtgtagGCtgaAGG | chr16 | 90227965 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tgCCCtCagCcCgCCcgCcCGGG | chr13 | 114353556 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GtgCCCtcagcCCgCcCgcCCGG | chr13 | 114353557 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | ccgCCCtcgCgatgCtCtcCGGG | chr13 | 114353464 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tcCgCCCTCgcgatgctCTCCGG | chr13 | 114353465 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | acttgaaTgacCttatgagaAGG | chr20 | 64330031 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tAttgCtTtgtttCtcagctTGG | chr20 | 64333338 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | cttgtgCTCtgCaCatatatTGG | chr20 | 64330056 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | agCttttTgagtgagtgCTCAGG | chr20 | 64333448 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GctttttgagtgagtGCtcaGGG | chr20 | 64333449 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GtgagtgctCAgggtGttctAGG | chr20 | 64333458 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tcttCCtagCAaaCtttCTtTGG | chr20 | 64330126 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | ctttggtaaagaatgaataaAGG | chr20 | 64330143 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | cttatCCaCagCtgaGCtcaCGG | chr20 | 64330169 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | ccaCagCTgagCtCacggTtAGG | chr20 | 64330174 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | cACagCtgagctCaCGgtTaGGG | chr20 | 64330175 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | cttCagtataAtgttGgCTgTGG | chr20 | 64333669 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | ttCagtaTaatgttgGCtgtGGG | chr20 | 64333670 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GgCtgtgggtttgtCaCagaTGG | chr20 | 64333684 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | agatggCTtttattacCtTaAGG | chr20 | 64333701 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tttCtatgtagattttgtTgAGG | chr20 | 64333732 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | ttCtatgTagAttttGttgaGGG | chr20 | 64333733 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tAaCaaaaattCtgtttaTgTGG | chr20 | 64333820 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | cAttCaagtCtggCacCacgTGG | chr20 | 64330017 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | ctCataaggtcattCaagTCTGG | chr20 | 64330027 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | agCaCaagttcCttCtCaTaAGG | chr20 | 64330041 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | aAtgaatTCagtaaaGttTCAGG | chr20 | 64333412 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | agtttgCTaggaagataaatGGG | chr20 | 64330118 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | aAgtttgctaggaagataaaTGG | chr20 | 64330119 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tttaCCaaagAaagtttgctAGG | chr20 | 64330130 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | cctaaCCgtgAgCtCagCTgTGG | chr20 | 64330174 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | cAgCCaacattataCtgaagGGG | chr20 | 64333666 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | acagCCaaCattatactgaaGGG | chr20 | 64333667 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | cACagCCaaCAttataCtgaAGG | chr20 | 64333668 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | atagaaaaaaACatacCtTaAGG | chr20 | 64333716 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tggaaaggaCACgggaagTCTGG | chrY | 57214618 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GgaaaggaCacgggaagtctGGG | chrY | 57214619 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tggCaCaggCcagCaGttgCTGG | chrY | 57214533 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | tACaCatgaacagaaagaagAGG | chrY | 57214657 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GAaagaagaggtCaaagaaaAGG | chrY | 57214669 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GAggtCaaagAaaagGCtgaCGG | chrY | 57214676 | + | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GcaaCtgctggCCtgtgCcaGGG | chrY | 57214530 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | agCaaCtgCtggCCtGtgcCAGG | chrY | 57214531 | - | 0 | 0
Seq0 | X | GACCCCCTCCACCCCGCCTCNNN | GtCtgaCTtCcagCaaCtgCTGG | chrY | 57214542 | - | 0 | 0

This is different from the output that i get on the hosted server at http://www.rgenome.net/cas-offinder/

Is there something wrong in the input? or Is there something that is dont post processing?

[pjb7687]

Duplicate #46