The set of E-value (-E) did not work.

[Jiulong-Zhao]

Hi, kofam_scan_colleagues:
Recently, I run the kofam_scan software using the following command line:
exec_annotation -o 5k+_assembled_hits_to_vHMMs_ko.txt 5k+_all_assemblied_contigs.part_329_prot.fasta -k ~/database/kofam/ko_list -p ~/database/kofam/profiles/ -E 0.00001 --cpu 5
But, it seems like the "-E 0.00001" did not work. The result file contained many hits which have the e-value higher than 0.00001.
I tried "-E 0.00001" and "-E 1.0e-05", but both can not work.
My input file was attached.
Thank you all and look forward to your reply.
5k+_all_assemblied_contigs.part_329_prot.txt

[David-Madrigal]

I'm having this exact same issue. Modifying the e-value do not change anything in the output file…

Did you manage to solve it somehow?

[francesco-ricci]

I am looking for an answer to this question too