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F = Number of times primer is in FG
B = Number of times primer is in BG
Ex = Exclusion penalty
C1 = First Scalar
C2 = -Scalar
Ra = Rolling Amplification (multiple primer sets are under the same stretch) = sum(Lfg) / sum(Lbg) BestSet = C1 * sum(Temp) / n + C2 * sum(Ex trues) + Ra
After completing formula, rank from best to worst (individual score), then split data into x bins, randomly select 1 - x number, then randomly select a value inside that bin, then calculate the bestSet score for that set.
Then run this about 10000 times, and select the best one.
The text was updated successfully, but these errors were encountered:
F = Number of times primer is in FG
B = Number of times primer is in BG
Ex = Exclusion penalty
C1 = First Scalar
C2 = -Scalar
Ra = Rolling Amplification (multiple primer sets are under the same stretch) = sum(Lfg) / sum(Lbg)
BestSet = C1 * sum(Temp) / n + C2 * sum(Ex trues) + Ra
After completing formula, rank from best to worst (individual score), then split data into x bins, randomly select 1 - x number, then randomly select a value inside that bin, then calculate the bestSet score for that set.
Then run this about 10000 times, and select the best one.
The text was updated successfully, but these errors were encountered: