This is the Huginn version of the EMU snakemake workflow. Basically, it's just two files, a minimal snakemake file that refers to the one in Github and a configuration file already "configured" to huginn. Please, refer to the public repo for more information.
- Preparate the metadata sheet.
- Run the pipeline
- Download the RDS file with a phyloseq object ready to analyze.
conda activate snakemake
snakemake --use-conda -n --conda-prefix /software/emu-snakemake
snakemake --use-conda -c50 --conda-prefix /software/emu-snakemake
If you want to keep intermediate output (such as unclassified sequences in a separate fasta or the alignments) use instead:
conda activate snakemake
snakemake --use-conda -n --conda-prefix /software/emu-snakemake --notemp
snakemake --use-conda -c50 --conda-prefix /software/emu-snakemake --notemp