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4. Deconvolution
To perform deconvolution (Richardson Lucy), you will need the PSFs. We have provided simulated PSFs for the Zeiss lattice lightsheet generated by Zen software here. In the napari-lattice inerface, tick Deconvolution and then click the box Click to Select PSFs for deconvolution
. You can choose a multichannel psf image in the first box or choose psfs for each channel in every box. The order of the PSF images/channels must follow the order of the image channels. To accelerate deconvolution, under Choose processing device
, select cuda_gpu
, which will use pycudadecon
library for deconvolution and requires you to have an NVIDIA GPU. The cpu
options uses Richardson-Lucy algorithm from scikit-image library. You can also define the number of iterations for running RL deconvolution.
You can couple deconvolution with Cropping
and Workflows
as well.