Reduce the speed and time for creating the lipid bilayer

[zjuradoq]

I propose to reduce the speed and time for creating the lipid bilayer from 18000 rcf for 15 min to 16000 rcf for 8 min. I found in a parallel test that more vesicles were seen at a lower centrifuge speed. I believe that the vesicles may be rupturing, as microscopy showed high background fluorescence.

This may have been pooled with PR #28.

[p-stano]

Hello, it is a good suggestion. Indeed various centrifugation speeds have been used by different groups.
Optimizing this parameter is rather easy using the background fluorescence as a measure of droplet rupture. The other parameter can be cloudness of the oil phase (when all droplets have been transfered, this appears more or less transparent). For letting TXTL reactions start only when vesicles are formed (not a premature start in the w/o droplets), centrifugation should be run at low temperature (4-6 degrees C).