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test_GenomicRegionSet.py
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import unittest
from rgt.GenomicRegionSet import *
from rgt.Util import OverlapType
"""Unit Test"""
class TestGenomicRegionSet(unittest.TestCase):
def region_sets(self, listA, listB):
""" Setting two GenomicRegionSets as self.setA and self.setB for each case test. """
self.setA = GenomicRegionSet('for Unit Test')
for i in range(len(listA)):
self.setA.add(GenomicRegion(chrom=listA[i][0], initial=listA[i][1], final=listA[i][2]))
self.setB = GenomicRegionSet('for Unit Test')
for i in range(len(listB)):
self.setB.add(GenomicRegion(chrom=listB[i][0], initial=listB[i][1], final=listB[i][2]))
def test_extend(self):
"""
Two empty sets
A : none
R : none
"""
self.region_sets([],
[])
self.setA.extend(100, 100)
self.assertEqual(len(self.setA.sequences), 0)
"""
One region
A : -----
R : ---------
"""
self.region_sets([['chr1', 5, 10]],
[])
result = self.setA
result.extend(4, 4)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 14)
"""
Many region
A : ----- ------ ----- -----
R : --------=--------- ------------------
"""
self.region_sets([['chr1', 5, 10], ['chr1', 15, 20], ['chr1', 40, 50], ['chr1', 65, 75]],
[])
result = self.setA
result.extend(5, 5)
self.assertEqual(len(result), 4)
self.assertEqual(result[0].initial, 0)
self.assertEqual(result[0].final, 15)
self.assertEqual(result[1].initial, 10)
self.assertEqual(result[1].final, 25)
self.assertEqual(result[2].initial, 35)
self.assertEqual(result[2].final, 55)
self.assertEqual(result[3].initial, 60)
self.assertEqual(result[3].final, 80)
"""
Many region in different chromosome
A : ----- ------ ----- -----
R : none
"""
self.region_sets([['chr1', 5, 10], ['chr2', 15, 20], ['chr3', 40, 50], ['chr4', 65, 75]],
[])
result = self.setA
result.extend(5, 5)
self.assertEqual(len(result), 4)
self.assertEqual(result[0].initial, 0)
self.assertEqual(result[0].final, 15)
self.assertEqual(result[0].chrom, 'chr1')
self.assertEqual(result[1].initial, 10)
self.assertEqual(result[1].final, 25)
self.assertEqual(result[1].chrom, 'chr2')
self.assertEqual(result[2].initial, 35)
self.assertEqual(result[2].final, 55)
self.assertEqual(result[2].chrom, 'chr3')
self.assertEqual(result[3].initial, 60)
self.assertEqual(result[3].final, 80)
self.assertEqual(result[3].chrom, 'chr4')
"""
One region
A : -----
R : ---------
"""
self.region_sets([['chr1', 100, 200]],
[])
result = self.setA
result.extend(10, 10, percentage=True)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 90)
self.assertEqual(result[0].final, 210)
def test_sort(self):
self.region_sets([['chr1', 15, 20], ['chr1', 40, 50], ['chr1', 65, 75], ['chr1', 5, 10]],
[])
self.setA.sort()
def test_intersect(self):
"""
Two empty sets
A : none
B : none
R : none
"""
self.region_sets([],
[])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
One empty set
A : -----
B : none
R : none
"""
self.region_sets([['chr1', 5, 10]],
[])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
A : none
B : -----
R : none
"""
self.region_sets([],
[['chr1', 5, 10]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
No overlapping
A : ------ --------- -------
B : ---- ------ ------
R : none
"""
self.region_sets([['chr1', 1, 5], ['chr1', 11, 20], ['chr1', 33, 38]],
[['chr1', 7, 9], ['chr1', 20, 25], ['chr1', 26, 31]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
End-to-end attach
A : ------ ------
B : ------
R : none
"""
self.region_sets([['chr1', 1, 5], ['chr1', 11, 20]],
[['chr1', 5, 11]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
No length attach
A : . .
B : . .
R : none
"""
self.region_sets([['chr1', 2, 2], ['chr1', 20, 20]],
[['chr1', 5, 5], ['chr1', 20, 20]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
Perfect overlapping
A : ------
B : ------
R : ------
"""
self.region_sets(
[['chr1', 1, 10], ['chr1', 500, 550], ['chr1', 600, 650], ['chr1', 700, 750], ['chr1', 725, 800]],
[['chr1', 1, 10], ['chr1', 500, 550], ['chr1', 600, 650], ['chr1', 700, 750], ['chr1', 725, 800]])
result = self.setA.intersect(self.setB, mode=OverlapType.OVERLAP, rm_duplicates=True)
self.assertEqual(len(result), 4)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 10)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 5)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 10)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 5)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 10)
"""
One overlapping region
A : ------
B : --------
R1: -- (overlap)
R2: ------ (original)
R3: (comp_incl)
"""
self.region_sets([['chr1', 1, 10]],
[['chr1', 7, 20]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 7)
self.assertEqual(result[0].final, 10)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 10)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
Two simple overlapping regions
A : ------- --------
B : -------------
R1: --- ---- (overlap)
R2: ------- -------- (original)
R3: (comp_incl)
"""
self.region_sets([['chr1', 1, 10], ['chr1', 26, 35]],
[['chr1', 7, 30]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 7)
self.assertEqual(result[0].final, 10)
self.assertEqual(result[1].initial, 26)
self.assertEqual(result[1].final, 30)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 10)
self.assertEqual(result[1].initial, 26)
self.assertEqual(result[1].final, 35)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
Two separately overlapping regions
A : ------- --------
B : ----- --------
R1: --- ---- (overlap)
R2: ------- -------- (original)
R3: (comp_incl)
"""
self.region_sets([['chr1', 1, 10], ['chr1', 26, 35]],
[['chr1', 7, 15], ['chr1', 30, 40]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 7)
self.assertEqual(result[0].final, 10)
self.assertEqual(result[1].initial, 30)
self.assertEqual(result[1].final, 35)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 10)
self.assertEqual(result[1].initial, 26)
self.assertEqual(result[1].final, 35)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
Many various overlapping (mixed)
A : ------------------ -------- ---------
B : ---- ------- ------ ----------
R1: -- ------- -- ---- --- (overlap)
R2: ------------------ -------- --------- (original)
R3: (comp_incl)
"""
self.region_sets([['chr1', 3, 30], ['chr1', 50, 60], ['chr1', 70, 85]],
[['chr1', 1, 5], ['chr1', 10, 19], ['chr1', 27, 35], ['chr1', 55, 75]])
result = self.setA.intersect(self.setB, mode=OverlapType.OVERLAP)
self.assertEqual(len(result), 5)
self.assertEqual(result[0].initial, 3)
self.assertEqual(result[0].final, 5)
self.assertEqual(result[1].initial, 10)
self.assertEqual(result[1].final, 19)
self.assertEqual(result[2].initial, 27)
self.assertEqual(result[2].final, 30)
self.assertEqual(result[3].initial, 55)
self.assertEqual(result[3].final, 60)
self.assertEqual(result[4].initial, 70)
self.assertEqual(result[4].final, 75)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 3)
self.assertEqual(result[0].initial, 3)
self.assertEqual(result[0].final, 30)
self.assertEqual(result[1].initial, 50)
self.assertEqual(result[1].final, 60)
self.assertEqual(result[2].initial, 70)
self.assertEqual(result[2].final, 85)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
Different chromosomes
A : chr1 -------
B : chr2 -------
R : none
"""
self.region_sets([['chr1', 1, 10]],
[['chr2', 1, 10]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 0)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
Completely included overlapping
A : ---------------------------
B : ---- ------ -----------
R1: ---- ------ ------ (overlap)
R2: --------------------------- (original)
R3: (comp_incl)
"""
self.region_sets([['chr1', 1, 50]],
[['chr1', 1, 5], ['chr1', 10, 19], ['chr1', 45, 60]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 3)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 5)
self.assertEqual(result[1].initial, 10)
self.assertEqual(result[1].final, 19)
self.assertEqual(result[2].initial, 45)
self.assertEqual(result[2].final, 50)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 50)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 0)
"""
A : ---- ------ -----------
B : ---------------------------
R1: ---- ------ ------ (overlap)
R2: ---- ------ ----------- (original)
R3: ---- ------ (comp_incl)
"""
self.region_sets([['chr1', 1, 5], ['chr1', 10, 19], ['chr1', 45, 60]],
[['chr1', 1, 50]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 3)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 5)
self.assertEqual(result[1].initial, 10)
self.assertEqual(result[1].final, 19)
self.assertEqual(result[2].initial, 45)
self.assertEqual(result[2].final, 50)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 3)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 5)
self.assertEqual(result[1].initial, 10)
self.assertEqual(result[1].final, 19)
self.assertEqual(result[2].initial, 45)
self.assertEqual(result[2].final, 60)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 5)
self.assertEqual(result[1].initial, 10)
self.assertEqual(result[1].final, 19)
"""
A : -------------- -------
------
B : ----- ----------------
R1: ----- ------- (overlap)
----
R2: -------------- ------- (original)
------
R3: ------- (comp_incl)
"""
self.region_sets([['chr1', 1, 50], ['chr1', 20, 40], ['chr1', 70, 80]],
[['chr1', 25, 45], ['chr1', 65, 95]])
result = self.setA.intersect(self.setB)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 25)
self.assertEqual(result[0].final, 45)
self.assertEqual(result[1].initial, 70)
self.assertEqual(result[1].final, 80)
result = self.setA.intersect(self.setB, mode=OverlapType.ORIGINAL)
self.assertEqual(len(result), 3)
self.assertEqual(result[1].initial, 20)
self.assertEqual(result[1].final, 40)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 50)
self.assertEqual(result[2].initial, 70)
self.assertEqual(result[2].final, 80)
result = self.setA.intersect(self.setB, mode=OverlapType.COMP_INCL)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 70)
self.assertEqual(result[0].final, 80)
def test_closest(self):
"""
Two empty sets
A : none
B : none
R : none
"""
self.region_sets([],
[])
result = self.setA.closest(self.setB)
self.assertEqual(len(result), 0)
# """
# One empty set
# A : -----
# B : none
# R : none
# """
# self.region_sets([['chr1',5,10]],
# [])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 0)
# """
# A : none
# B : -----
# R : none
# """
# self.region_sets([],
# [['chr1',5,10]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 0)
# """
# Overlapping within set
# A : -----====-----
# B : ----
# R : ----
# """
# self.region_sets([['chr1',1,10],['chr1',6,15]],
# [['chr1',6,10]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 2)
# """
# A : ----
# B : -----====-----
# R : -----====-----
# """
# self.region_sets([['chr1',6,10]],
# [['chr1',1,10],['chr1',6,15]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 1)
# """
# No overlapping
# A : ------ --------- -------
# B : ---- ------ ------
# R : ------
# """
# self.region_sets([['chr1',1,5],['chr1',11,20],['chr1',33,38]],
# [['chr1',7,9],['chr1',20,25],['chr1',26,31]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 3)
# # self.assertEqual(result[0].initial, 20)
# # self.assertEqual(result[0].final, 25)
# """
# End-to-end attach
# A : ------ ------
# B : ------
# R : ------
# """
# self.region_sets([['chr1',1,5],['chr1',11,20]],
# [['chr1',5,11]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 2)
# # self.assertEqual(result[0].initial, 5)
# # self.assertEqual(result[0].final, 11)
# """
# Perfect overlapping
# A : ------
# B : ------
# R : ------
# """
# self.region_sets([['chr1',1,10]],
# [['chr1',1,10]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 1)
# self.assertEqual(result[0].initial, 1)
# self.assertEqual(result[0].final, 10)
# """
# One overlapping region
# A : ------
# B : --------
# R : --------
# """
# self.region_sets([['chr1',1,10]],
# [['chr1',7,20]])
# result = self.setA.closest(self.setB)
# self.assertEqual(result[0].initial, 7)
# self.assertEqual(result[0].final, 20)
# """
# Two simple overlapping regions
# A : ------- --------
# B : -------------
# R : -------------
# """
# self.region_sets([['chr1',1,10],['chr1',26,35]],
# [['chr1',7,30]])
# result = self.setA.closest(self.setB)
# self.assertEqual(result[0].initial, 7)
# self.assertEqual(result[0].final, 30)
# """
# Two separately overlapping regions
# A : ------- --------
# B : ----- --------
# R : none
# """
# self.region_sets([['chr1',1,10],['chr1',26,35]],
# [['chr1',7,15],['chr1',30,40]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 2)
# """
# Many various overlapping (mixed)
# A : ------------------ -------- ---------
# B : ---- ------- ------ ----------
# R : none
# """
# self.region_sets([['chr1',3,30],['chr1',50,60],['chr1',70,85]],
# [['chr1',1,5],['chr1',10,19],['chr1',27,35],['chr1',55,75]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 4)
# """
# Different chromosomes
# A : chr1 -------
# B : chr2 -------
# R : chr2 -------
#
# """
# self.region_sets([['chr1',1,10]],
# [['chr2',1,10]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 0)
# """
# Completely included overlapping
# A : ---------------------------
# B : ---- ------ -----------
# R : ---- ------ -----------
# """
# self.region_sets([['chr1',1,50]],
# [['chr1',1,5],['chr1',10,19],['chr1',45,60]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 3)
# """
# A : ---- ------ -----------
# B : ---------------------------
# R : none
# """
# self.region_sets([['chr1',1,5],['chr1',10,19],['chr1',45,60]],
# [['chr1',1,50]])
# result = self.setA.closest(self.setB)
# self.assertEqual(result, False)
# """
# A : ---- ------ ---
# B : --- -----
# R : ---
# """
# self.region_sets([['chr1',1,5],['chr1',27,45],['chr1',85,95]],
# [['chr1',15,20],['chr1',55,65]])
# result = self.setA.closest(self.setB)
# self.assertEqual(len(result), 1)
# self.assertEqual(result[0].initial, 15)
# self.assertEqual(result[0].final, 20)
def test_remove_duplicates(self):
"""
A : ===== -----
R : ----- -----
"""
self.region_sets([['chr1', 1, 10], ['chr1', 1, 10], ['chr1', 15, 25]],
[])
self.setA.remove_duplicates()
result = self.setA
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 10)
self.assertEqual(result[1].initial, 15)
self.assertEqual(result[1].final, 25)
"""
A : =====--- -----
R : =====--- -----
"""
self.region_sets([['chr1', 1, 10], ['chr1', 1, 15], ['chr1', 20, 25]],
[])
self.setA.remove_duplicates()
result = self.setA
self.assertEqual(len(result), 3)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 10)
self.assertEqual(result[1].initial, 1)
self.assertEqual(result[1].final, 15)
self.assertEqual(result[2].initial, 20)
self.assertEqual(result[2].final, 25)
"""
A : ===== ----- ------ ====
R : ----- ----- ------ ----
"""
self.region_sets(
[['chr1', 1, 10], ['chr1', 1, 10], ['chr1', 15, 25], ['chr1', 30, 35], ['chr1', 40, 45], ['chr1', 40, 45]],
[])
self.setA.remove_duplicates()
result = self.setA
self.assertEqual(len(result), 4)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 10)
self.assertEqual(result[1].initial, 15)
self.assertEqual(result[1].final, 25)
self.assertEqual(result[2].initial, 30)
self.assertEqual(result[2].final, 35)
self.assertEqual(result[3].initial, 40)
self.assertEqual(result[3].final, 45)
def test_window(self):
"""
A : -------
B : ------[ 99 ] [ 199 ]---
window = 100
R : - only one base overlaps with extending A
"""
self.region_sets([['chr1', 200, 300]],
[['chr1', 1, 101], ['chr1', 499, 550]])
result = self.setA.window(self.setB, adding_length=100)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 100)
self.assertEqual(result[0].final, 101)
"""
A : -------
B : ------[ 99 ] [ 199 ]---
window = 200
R : ------ -
left-hand side is covered, and the right-hand side is only one base overlapped
"""
self.region_sets([['chr1', 200, 300]],
[['chr1', 1, 101], ['chr1', 499, 550]])
result = self.setA.window(self.setB, adding_length=200)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 1) # GenomicRegion.extend will choose 1 rather than 0
self.assertEqual(result[0].final, 101)
self.assertEqual(result[1].initial, 499)
self.assertEqual(result[1].final, 500)
"""
A : ---- ----
B : -------- ----
window = 1000 (default)
R : ---- ----
"""
self.region_sets([['chr1', 3000, 3500], ['chr1', 4000, 4500]],
[['chr1', 1500, 2500], ['chr1', 5000, 5500]])
result = self.setA.window(self.setB)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 2000)
self.assertEqual(result[0].final, 2500)
self.assertEqual(result[1].initial, 5000)
self.assertEqual(result[1].final, 5500)
"""
A : ---- ----
B : -------- ----
window = 2000
R : -------- ----
---- ----
window = 100
R : none
"""
self.region_sets([['chr1', 3000, 3500], ['chr1', 4000, 4500]],
[['chr1', 1500, 2500], ['chr1', 5000, 5500]])
result = self.setA.window(self.setB, adding_length=2000)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 1500)
self.assertEqual(result[0].final, 2500)
self.assertEqual(result[1].initial, 5000)
self.assertEqual(result[1].final, 5500)
result = self.setA.window(self.setB, adding_length=100)
self.assertEqual(len(result), 0)
def test_subtract(self):
"""
A : none
B : ------
R : none
"""
self.region_sets([],
[['chr1', 6, 15]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 0)
"""
A : ------
B : none
R : ------
"""
self.region_sets([['chr1', 6, 15]],
[])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 6)
self.assertEqual(result[0].final, 15)
"""
A : ------
B : ------
R : ---
"""
self.region_sets([['chr1', 1, 10]],
[['chr1', 6, 15]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 6)
"""
A : ------
B : ------
R : ---
"""
self.region_sets([['chr1', 6, 15]],
[['chr1', 1, 10]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 1)
self.assertEqual(result[0].initial, 10)
self.assertEqual(result[0].final, 15)
"""
A : ---
B : ---------
R : none
"""
self.region_sets([['chr1', 6, 10]],
[['chr1', 1, 15]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 0)
"""
A : ---------
B : ---
R : --- ---
"""
self.region_sets([['chr1', 1, 15]],
[['chr1', 6, 10]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 1)
self.assertEqual(result[0].final, 6)
self.assertEqual(result[1].initial, 10)
self.assertEqual(result[1].final, 15)
"""
A : ------
B : ------
R : none
"""
self.region_sets([['chr1', 6, 15]],
[['chr1', 6, 15]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 0)
"""
A : ---------- ------
B : ---------- ----
R : ------- ------
"""
self.region_sets([['chr1', 5, 30], ['chr1', 70, 85]],
[['chr1', 20, 50], ['chr1', 100, 110]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 5)
self.assertEqual(result[0].final, 20)
self.assertEqual(result[1].initial, 70)
self.assertEqual(result[1].final, 85)
"""
A : ------ -----
B : ------
R : ---- -----
"""
self.region_sets([['chr1', 20, 30], ['chr1', 35, 55]],
[['chr1', 10, 23], ['chr1', 100, 110]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 2)
self.assertEqual(result[0].initial, 23)
self.assertEqual(result[0].final, 30)
self.assertEqual(result[1].initial, 35)
self.assertEqual(result[1].final, 55)
"""
A : ch1 ---------------------
ch2 -------------------------
B : ch1 ------
ch2 ------
R : ch1 -------- -------
ch2 -------------------
"""
self.region_sets([['chr1', 0, 30000], ['chr2', 0, 35000]],
[['chr1', 20000, 23000], ['chr2', 31000, 35000]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 3)
self.assertEqual(result[0].initial, 0)
self.assertEqual(result[0].final, 20000)
self.assertEqual(result[1].initial, 23000)
self.assertEqual(result[1].final, 30000)
self.assertEqual(result[2].initial, 0)
self.assertEqual(result[2].final, 31000)
"""
A : -----------------------------------------------------------
B : --- --------- ---- ----
R : - ---- --------- ----------- --------------
"""
self.region_sets([['chr1', 5, 1000]],
[['chr1', 10, 15], ['chr1', 30, 70], ['chr1', 120, 140], ['chr1', 200, 240]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 5)
"""
By default, subtract merges any overlapping region. We represent the modified region set as A' below:
A : ----------------------- ------
----- ----- -----------
A': --------------------------------- ------
B : --- --------- ---- ----
R : - ---- --------- --- ----
"""
self.region_sets([['chr1', 5, 100], ['chr1', 20, 40], ['chr1', 60, 80], ['chr1', 95, 150], ['chr1', 180, 220]],
[['chr1', 10, 15], ['chr1', 30, 70], ['chr1', 120, 140], ['chr1', 200, 240]])
result = self.setA.subtract(self.setB)
res_list = [(r.initial, r.final) for r in result]
self.assertListEqual(res_list, [(5, 10), (15, 30), (70, 120), (140, 150), (180, 200)])
"""
When we disable merging, the result is usually bigger and the output regions will have different bounds:
A : ----------------------- ------
----- ----- -----------
B : --- --------- ---- ----
R : - ---- ------ ----
--- --- ---- ---
"""
self.region_sets([['chr1', 5, 100], ['chr1', 20, 40], ['chr1', 60, 80], ['chr1', 95, 150], ['chr1', 180, 220]],
[['chr1', 10, 15], ['chr1', 30, 70], ['chr1', 120, 140], ['chr1', 200, 240]])
result = self.setA.subtract(self.setB, merge=False)
res_list = [(r.initial, r.final) for r in result]
self.assertListEqual(res_list, [(5, 10), (15, 30), (70, 100), (20, 30),
(70, 80), (95, 120), (140, 150), (180, 200)])
"""
A : -----------------------------------------------------------
B : --- --------- ---- ----
R : - ---- --------- ----------- --------------
"""
self.region_sets([['chr1', 5, 1000], ['chr2', 5, 1000], ['chr4', 5, 1000]],
[['chr1', 10, 15], ['chr1', 30, 70], ['chr1', 120, 140], ['chr1', 200, 240],
['chr2', 10, 15], ['chr2', 30, 70], ['chr2', 120, 140], ['chr2', 200, 240],
['chr4', 10, 15], ['chr4', 30, 70], ['chr4', 120, 140], ['chr4', 200, 240]])
result = self.setA.subtract(self.setB)
self.assertEqual(len(result), 15)
# test exact subtraction
# the arguments merge and whole_region must be ignored, so we set their defaults in the most disrupting way:
# whole_region=False: allows partial subtraction (unless exact=True)
# merge=True: any overlapping regions in the two GRSs are merged (unless exact=True)
"""
Subtracting one region (and its duplicate), ignoring regions before/after and overlapping
A : ---
-----
----------
----------
------------------
-----
B : ----------
R : ---
-----
------------------
-----
"""
self.region_sets([['chr1', 10, 13], ['chr1', 13, 18],
['chr1', 13, 23], ['chr1', 13, 23],
['chr1', 15, 35], ['chr1', 25, 30]],
[['chr1', 13, 23]])
result = self.setA.subtract(self.setB, exact=True, merge=True, whole_region=False)
self.assertEqual(len(result), 4)
res_list = [(r.initial, r.final) for r in result]
self.assertListEqual(res_list, [(10, 13), (13, 18), (15, 35), (25, 30)])
"""
Subtracting two different but overlapping regions, and one duplicate region gets removed
A : ---
-----
----------
----------
------------------
-----
B : -----
------------------
R : ---
----------
-----
"""
self.region_sets([['chr1', 10, 13], ['chr1', 13, 18],
['chr1', 13, 23], ['chr1', 13, 23],
['chr1', 15, 35], ['chr1', 25, 30]],
[['chr1', 13, 18], ['chr1', 15, 35]])
result = self.setA.subtract(self.setB, exact=True, merge=True, whole_region=False)
self.assertEqual(len(result), 3)
res_list = [(r.initial, r.final) for r in result]
self.assertListEqual(res_list, [(10, 13), (13, 23), (25, 30)])
"""
Subtracting two different but overlapping regions that are on different chromosomes,
and one duplicate region gets removed
A : ---
-----
----------
----------
------------------
-----
B : ----- (chr5)
------------------ (chr9)
R : ---
-----
----------
------------------
-----
"""
self.region_sets([['chr1', 10, 13], ['chr1', 13, 18],
['chr1', 13, 23], ['chr1', 13, 23],
['chr1', 15, 35], ['chr1', 25, 30]],
[['chr5', 13, 18], ['chr9', 15, 35]])
result = self.setA.subtract(self.setB, exact=True, merge=True, whole_region=False)
self.assertEqual(len(result), 5)
res_list = [(r.initial, r.final) for r in result]
self.assertListEqual(res_list, [(10, 13), (13, 18), (13, 23), (15, 35), (25, 30)])
"""
Subtracting two different but not overlapping regions, and one duplicate region gets removed
A : ---
-----
----------
------------------
------------------
-----
B : --- -----
R : -----
----------
------------------
"""
self.region_sets([['chr1', 10, 13], ['chr1', 13, 18],