You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
The result of ipdSummary of reads that are mapped to a positive strand are shown to be in the reverse strand and vice versa:
My reference is mapped to the positive strand and I checked it with BLAST to make sure of it.
I have a bam file and I filter it using samtools to forward and reverse reads as follows:
REF=GCF_009914755.1_T2T-CHM13v2.0_genomic.fasta
min=0
max=45090682 # (I split the positions to multiple ranges in the actual script)
winid=20
ipdSummary chr21+.bam --reference $REF --referenceWindow $winid:$min-$max --identify m6A,m4C,m5C -j 30 --methylFraction --csv ch21+.csv --gff ch21+.gff
ipdSummary chr21-.bam --reference $REF --referenceWindow $winid:$min-$max --identify m6A,m4C,m5C -j 30 --methylFraction --csv ch21-.csv --gff ch21-.gff
In the results of chr21+.bam, in all '.gff' file the strand seems to be '-', and in the .csv files the strand is '1', and vice versa for the reverse strand. I checked my file to see if it was a result of a typo, but the SAM Flags of reads seem to be in the reverse strand in the chr21-.bam. I am wondering which part I might have done wrong. Am I using the ipdSummary not correctly? Is the --referenceWindow used correctly here? Something must be wrong, I would appreciate it if you could give me a hint specifically the usage of --referenceWindow argument.
The text was updated successfully, but these errors were encountered:
Marjan-Hosseini
changed the title
The results are mapped to a opposite strand, does the argument --referenceWindow remains the same for both strands?
The results are mapped to an opposite strand than their alignment, does the argument --referenceWindow remains the same for both strands?
Feb 17, 2024
The result of ipdSummary of reads that are mapped to a positive strand are shown to be in the reverse strand and vice versa:
My reference is mapped to the positive strand and I checked it with BLAST to make sure of it.
I have a bam file and I filter it using samtools to forward and reverse reads as follows:
Then I run ipdSummary on both of these files:
In the results of chr21+.bam, in all '.gff' file the strand seems to be '-', and in the .csv files the strand is '1', and vice versa for the reverse strand. I checked my file to see if it was a result of a typo, but the SAM Flags of reads seem to be in the reverse strand in the chr21-.bam. I am wondering which part I might have done wrong. Am I using the ipdSummary not correctly? Is the --referenceWindow used correctly here? Something must be wrong, I would appreciate it if you could give me a hint specifically the usage of --referenceWindow argument.
The text was updated successfully, but these errors were encountered: