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An awk script for split each amplicon data from multiple samples NGS fastq file.

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split_amplicon

An awk script for split each amplicon data from multiple samples NGS fastq file.

Just run this script as below line:

bash 1.extractFq_v0.2.sh path_to_your_barcode path_to_your_multiple_samples_fastq out_path

Attention: multiple_samples_fastq should be decompressed. The barcode file should be in Tab-Separated format. The barcoded primer contain 6 nt unique barcode, but we use more longer seq (contain protection bases around barcode) than 6nt can improve the specificity.

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An awk script for split each amplicon data from multiple samples NGS fastq file.

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