-
Notifications
You must be signed in to change notification settings - Fork 2
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
added script for generating napari 3D viewer screenshots
- Loading branch information
Showing
1 changed file
with
113 additions
and
0 deletions.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,113 @@ | ||
# %% | ||
from pathlib import Path | ||
|
||
import napari | ||
from brainglobe_utils.IO.image import load_any, load_nii | ||
|
||
# %% | ||
# Define input/output paths | ||
|
||
work_dir = Path.home() / "Dropbox/NIU/resources/figures/BlackCap_atlas_v1" | ||
out_dir = work_dir / "img" | ||
subject_id = "sub-BC73" | ||
subject_image_name = f"{subject_id}_asym-brain.nii.gz" | ||
subject_img_path = work_dir / "data" / subject_image_name | ||
|
||
brainglobe_dir = Path.home() / ".brainglobe" | ||
atlas_name = "oldenburg_blackcap_25um_v1.1" | ||
average_img_path = brainglobe_dir / atlas_name / "reference.tiff" | ||
|
||
# %% | ||
# Load the images | ||
|
||
subject_img = load_nii(subject_img_path, as_array=True, as_numpy=True) | ||
print("Loaded subject image: ", subject_image_name) | ||
print("Subject image shape: ", subject_img.shape) | ||
|
||
template_img = load_any(average_img_path) | ||
print("Loaded template image for ", atlas_name) | ||
print("Template image shape: ", template_img.shape) | ||
|
||
# %% | ||
# Start napari viewer | ||
viewer = napari.Viewer(ndisplay=3) | ||
|
||
# Shared options for image layers | ||
image_layer_opts = { | ||
"rendering": "attenuated_mip", | ||
"depiction": "volume", | ||
} | ||
|
||
# Define napari viewer angles for each camera view | ||
camera_views = dict( | ||
front=(0, 0, 90), | ||
back=(0, 180, 90), | ||
top=(180, 0, 0), | ||
bottom=(180, 180, 0), | ||
left=(0, 90, 90), | ||
right=(0, -90, 90), | ||
oblique=(20, -40, 60), | ||
) | ||
|
||
# %% | ||
# Add the average template image to the viewer | ||
template_layer = viewer.add_image( | ||
template_img, | ||
name="average_template", | ||
experimental_clipping_planes=[ | ||
{ | ||
"position": [210, 0, 0], | ||
"normal": [1, 0, 0], | ||
"enabled": True, | ||
} | ||
], | ||
**image_layer_opts, | ||
) | ||
|
||
# %% | ||
# Take screenshot of the viewer at each pre-defined angle | ||
for view_name, view_angles in camera_views.items(): | ||
template_layer.experimental_clipping_planes[0].enabled = ( | ||
True if view_name == "oblique" else False | ||
) | ||
template_layer.attenuation = 0.2 if view_name == "oblique" else 0.03 | ||
|
||
viewer.camera.angles = view_angles | ||
viewer.screenshot( | ||
out_dir / f"{template_layer.name}_view-{view_name}.png", | ||
canvas_only=True, | ||
) | ||
# Hide the template layer | ||
template_layer.visible = False | ||
|
||
# %% | ||
# Add the subject image to the viewer | ||
subject_layer = viewer.add_image( | ||
subject_img, | ||
name=subject_id, | ||
contrast_limits=[50, 2000], | ||
experimental_clipping_planes=[ | ||
{ | ||
"position": [205, 0, 0], | ||
"normal": [1, 0, 0], | ||
"enabled": True, | ||
} | ||
], | ||
**image_layer_opts, | ||
) | ||
|
||
# %% | ||
# Take screenshot of the viewer at each pre-defined angle | ||
for view_name, view_angles in camera_views.items(): | ||
subject_layer.experimental_clipping_planes[0].enabled = ( | ||
True if view_name == "oblique" else False | ||
) | ||
subject_layer.attenuation = 0.2 if view_name == "oblique" else 0.03 | ||
|
||
viewer.camera.angles = view_angles | ||
viewer.screenshot( | ||
out_dir / f"{subject_layer.name}_view-{view_name}.png", | ||
canvas_only=True, | ||
) | ||
|
||
# %% |