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Update 04-sequence_distance.md
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Czirion authored Sep 26, 2023
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Expand Up @@ -150,13 +150,13 @@ $ head -n4 output_blast/mini-genomes.blast
{: .output}

> ## Exercise 1: Remote blast search
> We already know how to perform a BLAST search of one FASTA file with many sequences to a custom database of the same sequences.
> What if we want to make a search against the available NCBI databases?
> 1) Search on the help page of `blastp` how you can do a remote search.
> 2) Search on the help page of `blastp` which other fields can be part of your tabular output.
> 3) Create a small FASTA file with only one sequence of one of our mini genomes.
> 4) Run `blastp` remotely against the `refseq_protein` database for the created FASTA file and add more fields to the output.
> (Note that adding the `qseqid` field will not be necessary because we are searching only one protein.
> We already know how to perform a BLAST search of one FASTA file with many sequences to a custom database of the same sequences.
> What if we want to make a search against the available NCBI databases?
> 1) Search on the help page of `blastp` how you can do a remote search.
> 2) Search on the help page of `blastp` which other fields can be part of your tabular output.
> 3) Create a small FASTA file with only one sequence of one of our mini genomes.
> 4) Run `blastp` remotely against the `refseq_protein` database for the created FASTA file and add more fields to the output.
> (Note that adding the `qseqid` field will not be necessary because we are searching only one protein.)
>
> > ## Solution
> > Use the command-line manual of `blastp`
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