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Workflow for Orthology analysis using UBLAST (faster, more sensitive than BLAST) and Reciprocal Best Hits

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Orthology-with-UBLAST

Workflow for Orthology analysis using UBLAST (faster, more sensitive than BLAST) and Reciprocal Best Hits

  • Find genome-wide orthologs with UBLAST (Linux bash script "ublast.sh") * the UBLAST algorithm implemented in USEARCH is faster and more sensitive than BLAST * USEARCH >> UBLAST documentation

  • Select the Best Reciprocal Hits from the UBLAST output (R script "RBH_ublast.R")

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Workflow for Orthology analysis using UBLAST (faster, more sensitive than BLAST) and Reciprocal Best Hits

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