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Update pVACview to point to updated anchor figure and vignette to match
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susannasiebert committed Sep 11, 2024
1 parent f19788c commit b804c0c
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10 changes: 8 additions & 2 deletions docs/pvacview/pvacseq_module/pvacseq_vignette.rst
Original file line number Diff line number Diff line change
Expand Up @@ -219,7 +219,11 @@ The candidate being investigated has a good binding affinity (median IC50 score
:alt: pVACview Vignette
:figclass: align-left

The mutation is not in an anchor position (see ``Anchor heatmap`` tab). Anchor prediction scores for each amino acid position are provided in the ``Anchor Weights`` tab at the bottom. Both mutant and wildtype peptides are good binders, yet the mutant peptide is a stronger binder. This is scenario number 2 (WT strong binder, MT strong binder, MT not in an anchor position) according to the Scenario Guide, where the neoantigen candidate is favorable and can be accepted.
The mutation is not in an anchor position (see ``Anchor heatmap`` tab). A list of anchor positions for each allele-length combination
is previded in the ``Anchor Positions`` panel below. Additional, the underlying anchor prediction scores for each amino acid position are
provided in the ``Anchor Weights`` panel at the bottom. Both mutant and wildtype peptides are good binders, yet the mutant peptide
is a stronger binder. This is scenario number 2 (WT strong binder, MT strong binder, MT not in an anchor position) according to the Scenario
Guide, where the neoantigen candidate is favorable and can be accepted.

.. figure:: ../../images/screenshots/vignette/KIF1C-new/KIF1C_5_AnchorHeatmap.png
:width: 1000px
Expand Down Expand Up @@ -330,7 +334,9 @@ The candidate also has good elution scores (elution scores close to 1). It's unc
:alt: pVACview Vignette
:figclass: align-left

Altogether, both the candidate (mutant peptide - MT) and its wildtype (WT) peptide are strong binders. The figure below shows the mutated amino acid (V) in the candidate is not in an anchor position. This fits into Scenario 4 in the guide, where the candidate is likely to elicit strong recognition from the immune system.
Altogether, both the candidate (mutant peptide - MT) and its wildtype (WT) peptide are strong binders. The figure below shows the mutated
amino acid (V) in the candidate (AERMGFTVV) is not in an anchor position for allele HLA-B*45:01. This fits into Scenario 4 in the guide,
where the candidate is likely to elicit strong recognition from the immune system.

.. figure:: ../../images/screenshots/vignette/ADAR/TranscriptSet1/ADAR_5_AnchorHeatmap_TranscriptSet1.png
:width: 1000px
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4 changes: 2 additions & 2 deletions pvactools/tools/pvacview/ui.R
Original file line number Diff line number Diff line change
Expand Up @@ -233,7 +233,7 @@ explore_tab <- tabItem(
) %>% withSpinner(color = "#8FCCFA"), style = "overflow-x: scroll; overflow-y: scroll",
column(width = 6,
h4("Anchor vs Mutation position Scenario Guide",
img(src = "https://github.com/griffithlab/pVACtools/raw/5834def4/pvactools/tools/pvacview/www/anchor.jpg",
img(src = "https://github.com/griffithlab/pVACtools/raw/f19788ccdb313a14a57350557cd70e1aa6bdca9a/pvactools/tools/pvacview/www/anchor.jpg",
align = "center", width = "100%")
)
)
Expand Down Expand Up @@ -579,7 +579,7 @@ tutorial_tab <- tabItem("tutorial",
p(strong("Scenario 4 : "), code(" I + III"), strong(" -> Accept"))
),
column(width = 6,
img(src = "https://github.com/griffithlab/pVACtools/raw/5834def4/pvactools/tools/pvacview/www/anchor.jpg",
img(src = "https://github.com/griffithlab/pVACtools/raw/f19788ccdb313a14a57350557cd70e1aa6bdca9a/pvactools/tools/pvacview/www/anchor.jpg",
align = "center", height = "350px", width = "600px"), br(), br()
)
)
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