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semiller10 committed Sep 27, 2024
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4 changes: 2 additions & 2 deletions anvio/docs/programs/anvi-draw-kegg-pathways.md
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Expand Up @@ -168,7 +168,7 @@ anvi-draw-kegg-pathways --external-genomes %(external-genomes)s \
-o output_dir
{{ codestop }}

The following map grid uncovers aspects of `Galactose metabolism` among the genomes of six *Enterococcus faecalis* strains. The unified map shows that some genomes may be missing steps from `Lactose` to `D-Tagatose-6P` in the tagatose-6-phosphate pathway, an alternative to the Leloir pathway for lactose catabolism found in some lactic acid bacteria. The individual maps show that strain ATCC29212 is missing three consecutive steps; OG1RF and V583 are missing two consecutive steps. When sequential reactions catalyzed by different enzymes are missing from a pathway in a closed genomic assembly while present in related genomes, it compellingly suggests that the genes and biological capacity are not in the genome rather than absent due to technical issues of orthology annotation.
The following map grid uncovers aspects of `Galactose metabolism` among the genomes of six *Enterococcus faecalis* strains. The unified map shows that some genomes may be missing steps from `Lactose` to `D-Tagatose-6P` in the tagatose-6-phosphate pathway, an alternative to the Leloir pathway for lactose catabolism found in some lactic acid bacteria. The individual maps show that strain **ATCC29212** is missing three consecutive steps; **OG1RF** and **V583** are missing two consecutive steps. When sequential reactions catalyzed by different enzymes are missing from a pathway in a closed genomic assembly while present in related genomes, it compellingly suggests that the genes and biological capacity are not in the genome rather than absent due to technical issues of orthology annotation.

![Map grid](../../images/anvi-draw-kegg-pathways/kos_database_grid.png)

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-o output_dir
{{ codestop }}

Continuing with the *Enterococcus* pangenome, the following map grid shows differences in `Pentose and glucuronate interconversions` between *faecalis* and *faecium* and between strains of each species. *faecalis* genomes are enriched in genes for xylose metabolism (towards the bottom of the map), and *faecium* genomes are enriched in enzymes for uronate metabolism (toward the top of the map). The *faecalis* MAG, "SHARON", has genes for the catabolism of `Xylose`, via `D-Xylulose` and `D-Xylulose-5P`. These are also annotated in three of the *faecalis* isolate genomes, ATCC29212, DENG1, and V583, but not the other three, D32, OG1RF, and Symbioflor_1. Xylose utilization is [known](https://doi.org/10.1186%%2Fs12864-015-1367-x) to be variable among *faecalis* strains.
Continuing with the *Enterococcus* pangenome, the following map grid shows differences in `Pentose and glucuronate interconversions` between *faecalis* and *faecium* and between strains of each species. *faecalis* genomes are enriched in genes for xylose metabolism (towards the bottom of the map), and *faecium* genomes are enriched in enzymes for uronate metabolism (toward the top of the map). The *faecalis* MAG, **SHARON**, has genes for the catabolism of `Xylose`, via `D-Xylulose` and `D-Xylulose-5P`. These are also annotated in three of the *faecalis* isolate genomes, **ATCC29212**, **DENG1**, and **V583**, but not the other three, **D32**, **OG1RF**, and **Symbioflor_1**. Xylose utilization is [known](https://doi.org/10.1186%%2Fs12864-015-1367-x) to be variable among *faecalis* strains.

![Pangenomic map grid](../../images/anvi-draw-kegg-pathways/kos_pan_grid.png)

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