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Assessing the purity of single cell population

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ROGUE (Ratio of Global Unshifted Entropy)

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Overview

Often, it is not even clear whether a given cluster is uniform in unsupervised scRNA-seq data analyses. Here, we proposed the concept of cluster purity and introduced a conceptually novel statistic, named ROGUE, to examine whether a given cluster is a pure cell population.

Installation Guide

Installing dependency package
Before installing ROGUE, the “tidyverse” package should be installed first:

install.packages("tidyverse")

Installing ROGUE
To install ROGUE, run:

if (!requireNamespace("devtools", quietly = TRUE)) install.packages("devtools")
devtools::install_github("PaulingLiu/ROGUE")

Tutorial

For more details and basic usage see following tutorials:

  1. Guided Tutorial (It takes a few seconds to load the HTML file)

Reproduction instructions

The scripts for producing all the quantitative results in our manuscript can be found in scripts.

Citation

If you use ROGUE in your research, please considering citing:

Contact

Please contact us:
Baolin Liu: [email protected]
Zemin Zhang: [email protected]

Copyright

©2019 Baolin Liu, Chenwei Li. Zhang Lab. All rights reserved.

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