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Update README.md
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shahab-sarmashghi authored Apr 24, 2019
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Expand Up @@ -36,7 +36,7 @@ Using Skmer
Skmer has three sub-commands:

### reference
Gets the path to a directory of FASTQ files (one uncompressed *.fastq/.fq* genome-skim per each sample) and creates a reference library containing the estimates of sequencing parameters as well as the Mash sketch for each genome-skim. Skmer also accepts FASTA files as input (one uncompressed *.fa/.fna/.fasta* file per each sample), where in that case the input is assumed to be a complete genome. All corrected pairwise genomic distances are then estimated and written to a file. For a test run, change the directory to `data` under your Skmer installation directory, and run
Gets the path to a directory of FASTQ/FASTA files (one uncompressed *.fastq/.fq/.fa/.fna/.fasta* file per each sample) and creates a reference library containing the estimates of sequencing parameters as well as the Mash sketch for each sample. If the input is an assembled sequence (determined by the length of sequences) the correction for low coverage and sequencing error is not applied to that sample. All corrected pairwise genomic distances are then estimated and written to a file. For a test run, change the directory to `data` under your Skmer installation directory, and run
```
skmer reference ref_dir -p 4
```
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